Publications

symptoms of poisoning after eating scarlet-runner beans.		1870	20745827
investigations on the nitrogenous metabolism of the higher plants: part iv. distribution of nitrogen in the dead leaves of the runner bean.		1922	16743123
investigations on the nitrogenous metabolism of the higher plants: part iii. the effect of low-temperature drying on the distribution of nitrogen in the leaves of the runner bean.		1922	16743122
investigations on the nitrogenous metabolism of the higher plants: part ii. the distribution of nitrogen in the leaves of the runner bean.		1922	16743087
investigations on the nitrogenous metabolism of the higher plants. part vii: leaf protein metabolism in normal and abnormal runner-bean plants.		1924	16743318
investigation on the nitrogenous metabolism of the higher plants. part v: diurnal variations in the protein nitrogen of runner bean leaves.		1924	16743316
reactions of valonia and of halicystis to colloids.	from the results of these tests it is clear that both halicystis and valonia have a high degree of tolerance for animal peptone, and a very high degree of tolerance for animal proteose and for egg albumen. the products of bacterial growths fostered by these proteins have a deleterious effect upon both species of algae; but, if it were possible to prevent bacterial growth entirely and at the same time supply proper food, it is probable that halicystis and valonia would show normal growth indefini ...	1933	19872750
maintained electrical polarities in region of the axillary buds in phaseolus multiflorus.		1936	16653348
further observations on the hemicelluloses of the pods of runner beans.		1937	16746561
bud regeneration and electrical polarities in phaseolus multiflorus.		1938	16653485
electrical response of phaseolus multiflorus to electrical currents.		1939	16653562
chromatographic and radioisotopic investigations of the lipid components of runner bean leaves.		1960	14404717
hydrolysis of monogalactosyl and digalactosyl diglycerides by specific enzymes in runner-bean leaves.		1964	14231719
lipid components of leaves. v. galactolipids, cerebrosides, and lecithin of runner-bean leaves.		1964	14229671
examination of the gibberellins of zea mays and phaseolus multiflorus using thin-layer chromatography.		1964	14210963
[on a bound gibberellin from phaseolus coccineus l].		1964	5852163
effect of hydrolytic enzymes on the photosynthetic efficiency and morphology of chloroplasts.	both lipase from runner beans and a protease (pronase) initially cause increased intensity dependence of the dcpip hill reaction of spinach chloroplasts. this is followed by an increase in the extrapolated zero intensity quantum requirement. pronase treatment of the chloroplasts causes rapid changes in absorption and ord spectra, whereas the effect of lipase treatment is much less pronounced. long treatments of the thylakoids with the lipase and protease cause unique morphological changes within ...	1966	16656445
[response-curves of the circadian rhythm in phaseolus].	in phaseolus multiflorus light interruptions were applied at different phases of the circadian cycles in leaf movements. the results were as follows. 1. the relation between the intensity of the light and the extent of the phase shift is qualitatively different with delays than it is with advances. these results suggest a difference in the nature of the primary processes in the two cases. 2. the response-curves for effects of white light (without far-red) published earlier are mainly due to the ...	1966	24557842
[peculiarities of circadian rhythms in plants from different geographical latitudes].	1 two species of plants (taraxacum arcticum and arnica angustifolia), collected in spitsbergen (geogr. latitude 76-80 degrees) exhibit endogenous circadian leaf movements but also movements with shorter periods. astragalus frigidus, a. alpinus and hedysarum hedysaroides, collected in arctic regions of continental europe, also show endogenous diurnal leaf movements. 2. in most of the species tested, there was no difference in the length of the free running periods of plants from arctic and centra ...	1966	24557984
translocation of indole-3-acetic acid-1'-c and tryptophan-1-c in seedlings of phaseolus coccineus l. and zea mays l.	indole-3-acetic acid-1'-(14)c (iaa-(14)c) and tryptophan-1-(14)c injected in small amounts into cotyledons of phaseolus coccineus l. seedlings were found to be translocated acropetally into the epicotyls and young shoots. similarly iaa-(14)c was translocated acropetally into coleoptiles of zea mays following injection into the endosperms. labeled metabolites of the injected compounds were also extractable from shoot tissue. however, evidence that iaa-(14)c itself was translocated acropetally was ...	1967	16656664
[further experiments concerning the influence of light on the circadian rhythm of phaseolus multiflorus].	shifting from dd (continuous darkness) to ll (continuous light; red with maximum at 660 nm) affects the circadian cycle of phaseolus as follows. 1. the cycle which is directly influenced by this energy step (called the first cycle), is either shortened ("advance") or lengthened ("delay") as compared with the control cycle of about 28 hours (fig. 1). the direction of this change, i.e., shortening or lengthening, is that which might be expected on the basis of the well known response curves. that ...	1967	24522501
autoradiographic evidence of tritiated indolyl-3-acetic acid in epicotyl tissue of phaseolus coccineus.		1967	6065783
distribution of gibberellin-like substances in light-and dark-grown seedlings of phaseolus multiflorus.	lettuce and barley endosperm bioassays of successive eluates from a phosphate-buffered celite column detected two gibberellin-like compunds, phaseolus i and phaseolus ii, in extracts of both light-and dark-grown seedlings of phaseolus multiforus. there were differences in the gibberellin content of light-and dark-grown seedlings, the former containing more phaseolus i but less phaseolus ii than the latter. an examination of the gibberellin content of leaves and apical buds, stems, cotyledons, an ...	1968	24519143
plant hormones. ix. phaseic acid, a relative of abscisic acid from seed of phaseolus multiflorus. possible structures.		1969	5373535
plant hormones. x. the constitution of phaseic acid; a relative of abscisic acid from phaseolus multiflorus. an interpretation of the mass spectrum of phaseic acid and a probable structure.		1969	5373536
[the importance of circadian leaf movements for the precision of day-length measurement].	1. in glycine max, the circadian leaf movements are synchronized with the 24 hr period in 12:12 hrs light-dark cycles even with light intensities of only 0.6-0.8 lx. the normal phase-angle difference between light-dark cycle and the circadian rhythm is already nearly reached with intensities of 10 lx. 2. in phaseolus multiflorus, light with the intensities of moonlight, offered during the dark-period of 12:12 hrs light-dark cycles, is able to shift the phases of the circadian leaf-movement rhyth ...	1969	24515819
the relative mitogenicities of some french bean (phaseolus vulgaris l.) and runner bean (phaseolus coccineus l.) seed extracts in leukocyte culture.		1971	5560539
characterization of gibberellins from dark-grown phaseolus coccineus seedlings by gas-liquid chromatography and combined gas chromatography-mass spectrometry.	an extract from 6000 dark-grown phaseolus coccineus seedlings was purified by countercurrent distribution and g-10 sephadex followed by gradient elution from a silicic acid partition column with increasing amounts of ethyl actetate in n-hexane. 25 fractions were collected and tested with the barley-aleurone, 'tan-ginbozu' dwarf-rice, lettuce, cucumber, dwarf-pea, d-1, d-2, d-3 and d-5 maize, oat first-internode, and sugarcane-spindle bioassays. major gibberellin (ga)-like activity was detected i ...	1971	24493224
effect of relative hormone concentration on auxin-gibberellin interaction in correlative inhibition of axillary buds.	indole-3-acetic acid (iaa) applied to the fully elongated second internode of decapitated phaseolus multiflorus plants always inhibited axillary bud elongation at concentrations down to 100 μg/g lanolin, whereas gibberellic acid (ga3) enhanced bud elongation at concentrations down to 1000 μg/g lanolin. lower concentrations than these of either iaa or ga3 were without significant effect. all possible combinations of iaa and ga3 within the concentration range 10(1) to 10(5) μg/g lanolin were antag ...	1971	24493039
the enzymic deacylation of phospholipids and galactolipids in plants. purification and properties of a lipolytic acyl-hydrolase from potato tubers.	an enzyme preparation that catalyses the deacylation of mono- and di-acyl phospholipids, galactosyl diglycerides, mono- and di-glycerides has been partially purified from potato tubers. the preparation also hydrolyses methyl and p-nitrophenyl esters and acts preferentially on esters of long-chain fatty acids. triglycerides, wax esters and sterol esters are not hydrolysed. the same enzyme preparation catalyses acyl transfer reactions in the presence of alcohols and also catalyses the synthesis of ...	1971	5154523
pattern of binding of tritiated actinomycin d to phaseolus coccineus polytene chromosomes. i. nucleolus organizing chromosomes.		1972	4635875
macromolecular synthesis during plant embryogeny: rates of rna synthesis in phaseolus coccineus embryos and suspensors.		1972	5074210
influence of valinomycin on circadian leaf movements of phaseolus.	phaseolus coccineus was exposed to valinomycin via the transpiration stream for 5-hr periods. the treatment started at various phases of the free-running circadian rhythm in continuous light; it resulted in phase shifts that varied in a manner dependent on the affected phases. the response curves are similar to those for transient withdrawal of water and for light pulses. the results support the hypothesis that membrane processes are important pacemakers in circadian rhythms.	1972	4506792
transport of indoleacetic acid in intact roots of phaseolus coccineus.	indoleacetic acid (iaa)-5-(3)h (2×10(-9)) was applied to intact roots of phaseolus coccineus seedlings at the apex or 2 cm above the apex, and the movement of iaa-(3)h and its metabolites traced by sectioning and chromatography. basipetal movement of label occurred for 2 cm or less, declining exponentially, and the amount increased with time. acropetal transport from above the apex showed quantitatively less movement of radioactivity. after a 6h treatment period a decline of label occurred in th ...	1972	24477753
[different light intensity dependence of the free-running periods as the cause of internal desynchronization of circadian rhythms in phaseolus coccineus].	the length of the free-running periods of circadian leaf movements in the primary and secondary pulvini of phaseolus coccineus was measured in constant darkness (dd) and continuous light (ll) of different intensities. the periods for the two pulvini do not differ from each other in dd and ll 30000 lx. however, they differ in ll 60 lx, 600 lx and 6000 lx, indicating a state of internal desynchronization. these results show a difference in the light intensity dependence of the 2 oscillations and a ...	1972	24473824
endogenous gibberellin transport and biosynthesis in relation to geotropic induction of excised sunflower shoot-tips.	surgical experiments on helianthus annuus and phaseolus multiflorus seedlings involving the application of auxin and gibberellin to decapitated plants, suggested that internode extension growth occurs under the controlling influence of apically synthesised gibberellin rather than auxin. studies were made of diffusible gibberellins from sunflower apical buds in relation to geotropic stimulation. approximately ten times as much gibberellin was obtained from lower than from upper tissues of horizon ...	1972	24477810
phytohemagglutinin: transitory enhancement of growth in phaseolus and allium.	phytohemagglutinin (difco) stimulates germination and early seedling growth in phaseolus coccineus, but not in ph. vulgaris, the species from which the compound is extracted. the differences to the controls reach a maximum at day 8, then they disappear. root growth in allium cepa is enhanced by phytohemagglutinin during the first 12 days of treatment, but only at temperatures below 20°c.	1972	24477998
rooted leaves for physiological experiments.	rooted leaves provide convenient material for investigating various physiological processes. details are given for producing rooted leaves of species with tuberous roots, e.g. sweet potato (ipomoea batatas) and dahlia (dahlia variabilis) and species with tap roots, e.g. sugar beet (beta vulgaris) and runner bean (phaseolus coccineus). the storage root formed on a single sugar-beet leaf develops concentric cambia suggesting that their formation is not determined by particular leaves.	1972	24481792
light-induced phase shifts of circadian leaf movements of phaseolus: comparison with the effects of potassium and of ethyl alcohol.	leaves of phaseolus coccineus were exposed to high concentrations of kcl (0.1-0.3 m) for periods of 4-5 hr, or to ethyl alcohol (5-30%) for periods of 2 hr. the treatment started at various phases of the free-running circadian rhythm in continuous light. whereas the k(+) pulses induced only advance phase shifts, i.e., shortening of the periods, the alcohol pulses caused stronger advances and also slight delays, i.e., lengthening of the periods. these delays became stronger with lower concentrati ...	1973	16592119
basipetally polarised transport of [(3)h]gibberellin a 1 and [ (14)c]gibberellin a 3, and acropetal polarity of [ (14)c]indole-3-acetic acid transport, in stelar tissues of phaseolus coccineus roots.	movement of both [(3)h]ga1 and [(14)c]ga3 through root segments from p. coccineus seedlings was basipetally polarised. the basipetal/acropetal ratio of radioactivity from [(3)h]ga1 in agar receiver blocks was 9.2 for apical, elongating segments, and 4.0 for more basal, non-elongating segments. polarity of gibberellin transport was restricted to the stele, and absent from cortical tissues. transport of [(14)c]iaa through root segments to agar receivers was preferentially acropetal, particularly s ...	1974	24442375
studies on phytohemagglutinins. xx. isolation and characterization of hemagglutinins from scarlet runner seeds (phaseolus coccineus l.).		1974	4854471
basipetal and acropetal transport of [3,4-(3)h]gibberellin a 1 in short and long segments of phaseolus coccineus second internode.	transport of [(3)h]ga1 occurred freely through stem segments ranging in length from 6 mm to 8 cm. only small amounts of radioactivity appeared in agar receiver blocks, particularly when transport in young, elongating, segments was measured. little or no metabolism of [(3)h]ga1 appeared to take place during a 16 h transport period. with 6 mm segments, there was no indication of polarity in longitudinal transport. in 8 cm long segments, an apparent acropetal polarity of [(3)h]ga1 transport occurre ...	1974	24458123
[transport and metabolism of 2-[(14)c]abscisic acid in root segments of phaseolus coccineus l].	movement of 2-[(14)c]aba through 1.5 cm and 5 cm long root segments of p. coccineus l. was acropetally polarised. the velocity of acropetal transport of [(14)c]aba in 1.5 cm long segments was 4-5 mm·h(-1). up to 11 h after the start of incubation [(14)c]aba could be extracted unchanged. beyond this time radioactivity became associated to an unidentified compound, which shows chromatographic qualities similar to those of milborrow's "metabolite c" (biochemistry & physiol. plant growth substances, ...	1974	24442705
translocation and metabolism of [(3)h]gibberellins by light-grown phaseolus coccineus seedlings.	tritiated gibberellins (gas) a1, a4, a5 and a20 injected into the apical bud of light-grown phaseolus coccineus l. seedlings were retained in the apical region and underwent little metabolism in 24 h. however, when injected into the hypocotyl, the [(3)h]gas were extensively metabolised and redistributed. all four gas were equally effective in promoting subapical elongation in shoot explants irrespective of apical or basal application. the metabolism and export of [(3)h]ga4 by excised organs incu ...	1975	24430472
growth regulator levels in embryo and suspensor of phaseolus coccineus at two stages of development.	gibberellins and auxins were extracted from embryos and suspensors of phaseolus coccineus l. at two stages of development: a) heart-shaped embryo and b) cotyledonary embryo with suspensor in the initial stage of degeneration. the time interval between the two stages was 5-6 days.in both embryos and suspensors, gibberellin (ga)-like activity was found in three fractions: f-1 (ethyl acetate fraction at ph 8.0), f-2 (free gas) and f-3 (bound gas). at stage a, the total ga activity in the suspensor ...	1975	24430372
[the effect of low temperature, nitrogen atmosphere and uncouplers on longitudinal distribution of radioactivity after acropetal and basipetal application of [2-(14)c] aba in long root segments of phaseolus coccineus l].	acropetal movement of [2-(14)c] abscisic acid (aba) in long root segments of p. coccineus is drastically reduced by destruction of the tissue, low temperature, anaerobic conditions and pretreatment with cccp (carbonyl-cyanide-m-chloro-phenylhydrazone) at uncoupling concentrations. basipetal transport is not affected by destruction of the tissue but markedly inhibited by the other treatments. consequently polarity of aba-transport disappears only after the roots have been killed. the importance o ...	1975	24430160
the effect of light on the gibberellin metabolism and growth of phaseolus coccineus seedlings.	light inhibits the rate of stem elongation of phaseolus coccineus l. seedlings. gibberellin a4 (ga4), an endogenous component of phaseolus seedlings (bowen et al., phytochem. 12, 2935-2941, 1973) promotes stem growth in the light but not in darkness. dark-grown seedlings contain larger ga pools than light-grown plants. apically applied [(3)h]ga4 in etiolated bolised more extensively in the light. the slower rate of metabolism of [(3)h]ga4 in etiolated seedlings is not a consequence of isotopic d ...	1975	24430090
relation between ribosomal rna genes and the dna satellites of phaseolus coccineus.		1975	1165210
[effect of light on transport of 2-[(14)c]abscisic acid through root segments of runner beans].	light promotes the acropetal movement of 2-[(14)c]aba through root segments of phaseolus coccineus l. the promotion occurs only when the segments are irradited during the transport period. red light and blue light are as effective as white light. there is no significant promotion when the segments are irradiated with green light or when they are kept in darkness during the transport period after preceding light treatment. light has no effect on basipetal aba-transport.	1975	24435922
[localisation of acropetal transport of 2-[(14)c]abscisic acid in roots of phaseolus coccineus l. and evidence for a radial aba-translocation between stele and cortex].	acropetal polarity of aba-transport in roots of runner beans is found to be located strictly in the stele. aba movement in the cortex is very slow and unpolar. experiments on partly decorticated and desteled roots gave evidence for a radial aba-transport between the stele and cortex. thus an aba distribution quotient stele/cortex of 12/1 (expressed on tissue volume basis) is established after 9 h aba incubation. the radial aba-translocation is strongly inhibited at low temperature.	1975	24435921
[period-lengthening and phase-shifting of the circadian rhythm of phaseolus coccineus l. by theorphylline].	under the continuous influence of theophylline the period of the circadian leaf movement rhythm of phaseolus is lengthened under conditions of continuous darkness and under conditions of continuous light. four-h pulses of theophylline shift the rhythm as a function of the phase.it is hypothesized that these theophylline effects are based not upon inhibition of the phosphodiesterase which hydrolyses 3',5'-camp but on the influence on membrane function, particularly the transport and binding of ca ...	1975	24435338
ammonia assimilation by rhizobium cultures and bacteroids.	the enzymes involved in the assimilation of ammonia by free-living cultures of rhizobium spp. are glutamine synthetase (ec. 6.o.i.2), glutamate synthase (l-glutamine:2-oxoglutarate amino transferase) and glutamate dehydrogenase (ed i.4.i.4). under conditions of ammonia or nitrate limitation in a chemostat the assimilation of ammonia by cultures of r. leguminosarum, r. trifolii and r. japonicum proceeded via glutamine synthetase and glutamate synthase. under glucose limitation and with an excess ...	1975	234505
metabolism of h-gibberellin a(1) and h-gibberellin a(4) by phaseolus coccineus seedlings.	[(3)h]-gibberellin a(1) (ga(1)) and (3)h-ga(4) were applied separately to phaseolus coccineus seedlings grown under red light. (3)h-ga(1) was converted to a compound with gas-liquid radiochromatography retention times identical to those of ga(8). (3)h-ga(4) underwent conversion to at least three metabolites, none of which corresponded to ga(1-38). the rate of metabolism of (3)h-ga(4) was significantly higher than that of (3)h-ga(1).	1975	16659025
immunosuppressive activity of phaseolus coccineus and phaseolus vulgaris extracts in mice.	lectin mixtures from 10 samples of phaseolus coccineus and phaseolus vulgaris beans were used as immunosuppressive agents in the sheep red blood cell (srbc) antibody response of the mouse. some bean samples contain lectins capable of inducing several times better suppression of humoral immunity than concanavalin a and phytohemagglutinin tested before in the same immune system. high dilutions of the 10 bean extracts were shown to agglutinate mouse, rabbit and sheep erythrocytes in vitro but the t ...	1976	1086791
[comparative studies on the reactive sites against trypsin of some inhibitors from phaseolus coccineus and phaseolus vulgaris (author's transl)].	three of the trypsin chymotrypsin inhibitors from the seeds of runner beans (phaseolus coccineus l.), pci 3,4(2), and 5, and three of the inhibitors from the seeds of french beans (phaseolus vulgaris var. nanus), pvi 3, 4, and 5, contain a lysine residue in the reactive site against trypsin. one of the inhibitors from phaseolus coccineus, pci 2, contains an arginine residue there. all seven phaseolus inhibitors investigated are double headed.	1976	1007623
[the basipetal transport of [2-(14)c] abscisic acid in roots of intact seedlings of runner beans and its significance for root geotropism].	one h and 3 h after point source application of [2-(14)c] aba to the upper side of geotropically stimulated root tips of intact seedlings of phaseolus coccineus l. a weak downward lateral transport of radioactivity was observed. a clear upward lateral translocation, however, occurred when aba was applied to the lower side of the root tip. thus a uniform distribution of radioactivity was established in horizontal roots regardless of the site of the application point, while in upright roots the di ...	1976	24430607
[extrasynthesis of dna and its inhibition by means of a rifampicin derivative in suspension cells of the phaseolus coccineus embryo [proceedings].		1976	1025656
suspensor, gibberellin and in vitro development of phaseolus coccineus embryos.	embryos of phaseolus coccineus in different stages of development (from 0.5 to 5 mm in length) were grown in vitro. both intact embryos (with suspensor) and embryos deprived of suspensor were studied. it was found that removal of the suspensor has no effect on the development of embryos which have reached a length of 5 mm. with younger embryos, removal of the suspensor reduces embryo development, the negative effect being the greater the younger the embryo. it was shown that gibberellic acid (ga ...	1976	24424756
[trypsin and chymotrypsin inhibitors in leguminosae vii. partial amino acid sequences of the trypsin chymotrypsin inhibitor pci 3 from phaseolus coccineus (author's transl)].	the sequences of amino acid residues at the amino and carboxyl terminus and around the reactive sites of the trypsin chymotrypsin inhibitor pci 3 from the seeds of runner beans (phaseolus coccineus l.) were estimated by aminopeptidase o and carbosypeptidase a degradation before and after enzymatical modification with trypsin or chymotrypsin. beginning at the amino terminus the sequences are :ser-glu-ala-gly-gln-...,...-ile-tyr-lys-ser-gln-(pro)-...with lys-ser as reactive site against trypsin, . ...	1976	1007624
membrane potentials and ion permeabilities in flexor cells of the laminar pulvini of phaseolus coccineus l.	the internal potential of flexor cells in slices of the laminar pulvini of phaseolus coccineus has been recorded by standard microelectrode techniques in 100 eq m(-3) external salt solutions of various ionic compositions. the measured values are between-15 and-60 mv depending on the external medium. treating the results with the goldman equation yields the following relative permeabilities: k(+), 1.00; na(+), 0.24; cl(-), 0.19; no 3 (-) , 1.6. the membrane potential was only slightly sensitive t ...	1977	24420512
abscisic acid and apical dominance in phaseolus coccineus l.	abscisic acid (aba) in lanolin, applied to the internode of decapitated runner bean plants enhances the outgrowth of lateral buds. the optimum concentration of the paste is 10(-5) m. the effect of aba is counteracted by indoleacetic acid (iaa) but not by gibberellic acid (ga3). there is no effect when aba is applied to the apical bud or lateral buds of intact plants. however, 13.2 ng given to the lateral buds of decapitated plants stimulate their growth, whereas higher concentrations are inhibit ...	1977	24419785
purification and properties of a plant endonuclease specific for apurinic sites.	an endonuclease which hydrolyzes depurinated dna has been isolated from phaseolus multiflorus enbryos; it has a molecular weight around 40,000. the enzyme is specific for apurinic sites; it has no action on normal dna strands or on alkylated sites, and is without exonulcease activity. the rate of phosphoester bond hydrolysis near apurinic sites is far greater in native than in denatured dna. the endonuclease is not inactivated by 10 mm edta, but is activity is however stimulated by mg2+ or mn2+. ...	1977	16891
cytological localization of the genes for the four classes of ribosomal rna (25s, 18s, 5.8s and 5s) in polytene chromosomes of phaseolus coccineus.	homologous tritiated 25s, 18s and 5.8s rrnas were used separately for in situ hybridization to the polytene chromosomes of the embryo suspensor cells of phaseolus coccineus. hybridization occurred at the same chromosomal sites which were labeled in previous in situ hybridization experiments with 25 + 18s rrnas in the same material (avanzi et al., 1972), namely: nucleolus organizing system (satellite, nucleolar constriction and organizer) of chromosome pairs i (s1) and v (s2), proximal heterochro ...	1977	858263
differences between adenosine triphosphatases from monocotylous and dicotylous plants.	the mitochondrial membrane-bound atpases of several plants were investigated. two distinct types were encountered. the mitochondrial atpases of castor bean (ricinus communis var. zanzibarensis, l.), cauliflower (brassica oleracea, l.), and scarlet runner (phaseolus coccineus, l.) were found to be inhibited by oligomycin, to have elevated molecular weights when separated from the organelles by ultrasonication and ammonium sulfate treatment and, subsequent to purification, to be cold-labile. on th ...	1977	16659806
distinction between nuclear satellite dnas and chloroplast dna in higher plants.	triton x-100 solubilized chloroplast dna but not nuclear dna from a mixture of chloroplasts and nuclei. the buoyant density of chloroplast dna was different from that of the satellite dna in all of the species examined (phaseolus coccineus, cucumis sativus, cucumis melo, antirrhinum majus, vicia faba, oenothera fruiticosa youngii). chloroplast dna constituted between 4.3% and 0.25% of the total leaf dna in these species, and was present as 5 to 20 copies in each chloroplast.	1978	16660649
isolation and chemical characterization of a highly purified phytomitogen from phaseolus coccineus seeds.	an effective lymphocyte mitogen with a low erythroagglutinating ability was isolated from the seeds of phaseolus coccineus l. by ammonium sulphate precipitation and repeated ion-exchange chromatography on modified sephadex. the mitogen stimulated lymphocytes at concentrations as low as 0.25 microgram/ml, the optimum concentration being in the range of 2.5-5.0 microgram/ml. this stimulating effect was completely inhibited by 5 mg/ml methyl alpha-d-mannopyranoside. column gel filtration, disc elec ...	1978	627207
embryo-suspensor relations in phaseolus coccineus: cytokinins during seed development.	data are presented on the cytokinin status of seeds and seed components, at different stages of development in phaseolus coccineus l., as determined with the soybean callus growth bioassay: a change in cytokinin types according to developmental stage occurred: from biologically very active less polar types (zeatin=z) at early stages to more polar types (zeatin glucoside=z9g and zeatin riboside=zr), with relatively low biological activity, at intermediate and late stages of seed development: when ...	1978	24408261
cap formation on lymphocytes from patients with leukemic diseases induced by four different lectins.	when ficoll purified peripheral blood lymphocytes were treated with fluorescein conjugated lectins from lentils (lch), castor beans (rca) and phaseolus coccineus beans (l-and e-pha) for 15 min and the percentages of the cap forming cells were examined, the values of leukemic lymphocytes were reduced compared to the values obtained with normal lymphocytes. the reduction was more than half in patients with acute and chronic myelogenous leukemia and immunoblastoma, it was only one quarter in patien ...	1978	274161
on the receptors of human red cells reacting with phaseolus coccineus l. lectin.	in order to elucidate receptors of proteolytic enzyme-treated red cells which react with phaseolus coccineus l. lectin, the receptors prepared by affinity chromatography were serologically investigated. p. coccineus lectin had high agglutinin activity for bromelin-, papain- and pronase-treated red cells but that for the cells treated with ficin and trypsin was relatively low. analyses of chemical composition revealed that sialic acid of the receptors from normal red cells was considerably much a ...	1979	494265
the lectin binding sites on the membranes of the nuclear envelope, mitochondria and the cell surface of human lymphoblastoid cells.	the membranes of the cell surface, the endoplasmic reticulum, outer and inner mitochondrial leaflet and nuclear envelope were isolated from three human lymphoblastoid cell lines. membrane components were separated by dodecyl sulfate polyacrylamide gel electrophoresis and the gels incubated with the radioiodinated lectins from lentil, castor bean, scarlet runner bean, gorse seed and roman snail. after gel slicing and counting, the molecular weights of the lectin binding sites were determined. abo ...	1979	527941
identification of traumatin, a wound hormone, as 12-oxo-trans-10-dodecenoic acid.	12-oxo-trans-10-dodecenoic acid (trans-10-oda) is an oxidation product of polyunsaturated fatty acids in plant tissues. the structural similarity of trans-10-oda and traumatic acid, a compound considered to be a wound hormone, suggested that trans-10-oda might be a precursor of traumatic acid. both trans-10-oda and traumatic acid were active in the wehnelt bean assay. the results were more consistent with trans-10-oda than with traumatic acid. cucumber (cucumis sativus l. var. national pickling) ...	1979	16660762
identification of gibberellin a1 in the embryo suspensor of phaseolus coccineus.	by combined gas chromatography-mass spectrometry the gibberellin present in suspensors of heart-shaped embryos of phaseolus coccineus has been identified as gibberellin a1 (ga1). the amount of ga1 in 2000 suspensors (452 mg), as estimated by gas chromatography. was 4μg. the presence of ga1 in suspensors of p. coccineus is discussed in relation to our present knowledge of the occurrence of many gibberellins in developing seeds and immature fruits of the same species.	1979	24311036
cytokinins and in vitro development of phaseolus coccineus embryos.	phaseolus coccineus embryos at the heartshaped and the middle cotyledonary stages were cultured in vitro on media added with different concentrations of zeatin (z) or zeatin riboside (zr). growth of early embryos was clearly favored by concentrations of z from 10(-8) m to 10(-5) m, lower concentrations having no effect. zr also promoted in vitro growth of early embryos, but in concentrations from 10(-12) m to 10(-10) m, higher concentrations being inhibitory. more developed embryos were scarcely ...	1979	24310890
fatty acids and circadian rhythms in phaseolus coccineus: effects of light, temperature, and chemicals.	five major fatty acids, palmitic (16:0), stearic (18:0), oleic (18:1), linoleic (18:2), and linolenic (18:3), were identified in polar lipid extracts from pulvini of samanea saman and phaseolus coccineus. in p. coccineus their distribution varied quantitatively in the laminar pulvinus, petiolar pulvinus, petiole, stem, leaf and root. short pulses of red light did not greatly affect the relative quantities of fatty acids in dark grown p. coccineus, but a 30-minute exposure of red light generally ...	1979	16660990
[acute poisoning with green beans (phaseolus vulgaris and phaseolus coccineus) (author's transl)].		1979	471530
distribution and properties of a potassium-dependent asparaginase isolated from developing seeds of pisum sativum and other plants.	asparaginase (ec 3.5.1.1) was isolated from the developing seed of pisum sativum. the enzyme is dependent upon the presence of k(+) for activity, although na(+) and rb(+) may substitute to a lesser extent. maximum activity was obtained at k(+) concentrations above 20 millimolar. potassium ions protected the enzyme against heat denaturation. the enzyme has a molecular weight of 68,300.asparaginase activity developed initially in the testa, with maximum activity (3.6 micromoles per hour per seed) ...	1980	16661136
a study of abscisic acid uptake by apical and proximal root segments of phaseolus coccineus l.	1. we investigated the ph and concentration dependence of abscisic acid uptake by short segments taken from different zones along the length of primary roots of phaseolus coccineus l. (runner bean). tissue from all regions studied, up to and including the zone of lateral root initiation showed a non-saturable uptake component identifiable with passive diffusion of the undissociated species of abscisic acid. the net uptake increased through the elongation zone towards the apex, perhaps principall ...	1980	24306804
electrical membrane properties and circadian rhythm in extensor cells of the laminar pulvini of phaseolus coccineus l.	circadian changes of three parameters of electrical properties in extensor cells of the laminar pulvini of phaseolus coccineus l. have been recorded by standard microelectrode techniques. in the low turgor state of the cells, the resting potential and the cn(-) (1 millimolar nacn) induced depolarizations are large, while the k(+)-induced voltage changes are small, and vice versa in the high turgor state. these results demonstrate that during a circadian cycle the low turgor state of the extensor ...	1980	16661315
glycoproteins from the cell wall of phaseolus coccineus.	1. the use of a modified sodium chlorite/acetic acid delignification procedure for the solubilization of a hydroxyproline-rich glycoprotein fraction from the depectinated cell walls of phaseolus coccineus is described. 2. the crude glycoprotein was associated with some pectic material; hydroxyproline and serine were the most abundant amino acids, and arabinose, galactose and galacturonic acid the predominant monosaccharides. 3. the bulk of the hydroxyproline is o-glycosidically substituted with ...	1980	7406871
energy-dependent phases of the circadian clock and the clock-controlled leaf movement in phaseolus coccineus l.	the energy requirements of the various phases of the circadian clock in the laminar pulvini cells of primary leaves of phaseolus coccineus l. were investigated using 4-h pulses of nacn (5 mm) and nan3 (1 mm). the induced phase shifts were calculated from the timing of the subjective night position during the third cycle after the treatment. both inhibitors produce advances during phases which are correlated with the upward movement of the leaf (ca. 0-12 h after the maximum of the subjective nigh ...	1981	24301022
covalent labelling of the nadph: protochlorophyllide oxidoreductase from etioplast membranes with [3h]n-phenylmaleimide.	[3h]n-phenylmaleimide has been used to covalently label in a specific manner the substrate-protected thiol groups of the enzyme protochlorophyllide reductase. in membrane preparations from oat (avena sativa) and runner-bean (phaseolus vulgaris) seedlings, two related peptides of mol.wts. 35000/37000 and 34000/35000 respectively and showing properties expected of the reductase have been identified, whereas the same technique with barley (hordeum vulgare) extracts resulted in labelling a single pe ...	1981	7306063
n abundance of nodules as an indicator of n metabolism in n(2)-fixing plants.	this paper expands upon previous reports of (15)n elevation in nodules (compared to other tissues) of n(2)-fixing plants. n(2)-fixing nodules of glycine max (soybeans), vigna unguiculata (cowpea), phaseolus vulgaris (common bean), phaseolus coccineus (scarlet runner bean), prosopis glandulosa (mesquite), and olneya tesota (desert ironwood) were enriched in (15)n. nodules of vicia faba (fava beans), arachis hypogaea (peanut), trifolium pratense (red clover), pisum sativum (pea), lathyrus sativus ...	1982	16662517
purification and partial characterization of an agglutinin from phaseolus coccineus var. 'alubia'.	an agglutination from seeds of 'alubia', a mexican strain of phaseolus coccineus, has been purified by affinity chromatography using physically entrapped stroma. the protein appears to be homogeneous by electrophoresis, molecular sieve chromatography and ultracentrifugation. a molecular species of approx. mr 112,000, with s values of 6.25, 4.52, 4.63 and 4.65 at ph 2.5, 4.5, 7.0 and 9.5, respectively, consisting of four similar subunits (28 kda), and containing 20% w/w glucosamine, is found to b ...	1982	7126589
carriers for abscisic acid and indole-3-acetic acid in primary roots: their regional localisation and thermodynamic driving forces.	a carrier for the uptake of abscisic acid (aba) is present in the tips and elongating zones of primary roots of both leguminous (runner bean, french bean, pea) and non-leguminous (sunflower, maize) seedlings. no aba carrier was present in more mature root regions. for indole-3-acetic acid both carrier-mediated uptake and a 2,3,5-triiodobenzoate-sensitive efflux component are present in growing and in non-elongating runner-bean root tissues. both aba and indole-3-acetic acid carriers were inactiv ...	1983	24263945
a survey of the nutritional and haemagglutination properties of legume seeds generally available in the uk.	eighty-five samples from fifteen different legume seed lines generally available in the uk were examined by measurements of their net protein utilization by rats and by haemagglutination tests with erythrocytes from a number of different animal species. from these results the seeds were classified into four broad groups. group a seeds from most varieties of kidney (phaseolus vulgaris), runner (phaseolus coccineus) and tepary (phaseolus acutifolius) beans showed high reactivity with all cell type ...	1983	6615758
flow cytometric analysis of the binding of eleven lectins to human t- and b-cells and to human t- and b-cell lines.	the relative surface binding of 11 lectins to human peripheral blood t- and b-lymphocytes, to molt-4 and jm t-cell lines, and to 6410 and nc37 b-cell lines was determined by flow cytometry. the lectins from lens culinaris (lca), ricinus communis (rca), arachis hypogaea (pna), abrus precatorius (apa), ulex europaeus (uea-f), sarothamnus scoparius (sas-f), helix pomatia (hpa), phaseolus coccineus (l-pha), glycine max (sba), and triticum vulgare (wga) were fluoresceinated and incubated with living, ...	1984	6609053
gibberellin-like activity in suspensors of tropaeolum majus l. and cytisus laburnum l.	gibberellins in the embryo-suspensor system have been considered so far only in phaseolus coccineus. we present in this report the localization of gibberellin-like substances in the suspensors of tropaeolum majus l. and cytisus laburnum l. the total gibberellin activity (expressed as gibberellic-acid equivalent in the α-amylase bioassay) in 2000 suspensors (106 mg fresh weight; fw) of c. laburnum and in 600 suspensors (236 mg fw) of t. majus were 50.9 μg g(-1) fw and 8.9 μg g(-1) fw respectively ...	1984	24253275
identification and localization of gibberellins in maturing seeds of the cucurbit sechium edule, and a comparison between this cucurbit and the legume phaseolus coccineus.	twenty known gibberellins (gas) have been identified by combined capillary gas chromatography-mass spectrometry in extracts from less than 10 g fresh weight of maturing seeds of the cucurbit sechium edule sw. the gas are predominantly 3- and-or 13-hydroxylated. this is the first reported identification of non-conjugated 13-hydroxylated gas in a cucurbit. gibberellin a8 and gibberellin a8-catabolite are the major gas in terms of quantity and are largely accumulated in the testa. the catabolites o ...	1984	24253274
in situ evaluation of the functional state of chromatin by means of quinacrine mustard staining and time-resolved fluorescence microscopy.	a pulsed laser microfluorometer with high spatial and temporal resolution was employed to study the functional state of chromatin, using quinacrine mustard as the fluorescent probe. corresponding segments of polytene chromosomes of embryo suspensor cells of phaseolus coccineus and parenchymal cells of helianthus tuberosus, in phases not involving dna synthesis, were selected as models. the fluorescence decay time turned out to be a discriminating parameter for the chromatin fractions of differin ...	1984	6199331
aged preparations are electrophysiologically more representative of intact tissue than are freshly excised preparations.	studies using excised tissues usually suffer from a discrepancy in the results between freshly excised preparations and "aged" ones (i.e. preparations which are allowed to rest and-or regenerate for about a day between excision and experimentation). this is especially true for measurements of the membrane potential (vm) and its changes upon exposure to light in slices of extensor tissue of the laminar pulvini of phaseolus coccineus l. measurements of the v m of extensor cells in situ reveal virt ...	1984	24258377
modulation of carrier-mediated uptake of abscisic acid by methyl jasmonate in phaseolus coccineus l.	the effects of methyl jasmonate and jasmonic acid on uptake of abscisic acid (aba) by suspension-cultured runner-bean cells and subapical runner-bean root segments have been investigated. increasing concentrations of methyl jasmonate inhibit aba uptake by the cultured cells with a k i of 22±3 μm. this is not due to cytoplasmic acidification or to effects on metabolism of aba, and is not additive with inhibition of radioactive aba uptake by nonradioactive aba. uptake of indol-3-yl acetic acid (ia ...	1985	24241440
conversion of [(14)c]gibberellin a 12-aldehyde to c 19- and c 20-gibberellins in a cell-free system from immature seed of phaseolus coccineus l.	a cell-free system prepared from developing seed of runner bean (phaseolus coccineus l.) converted [(14)c]gibberellin a12-aldehyde to several products. thirteen of these were identified by capillary gas chromatography-mass spectrometry as gibberellin a1 (ga1), ga4, ga5, ga6, ga15, ga17, ga19, ga20, ga24, ga37, ga38, ga44 and ga53-aldehyde, all giving mass spectra with (14)c-isotope peaks. ga8 and ga28 were also identified but contained no (14)c. all the [(14)c]ga12-aldehyde metabolites, except g ...	1985	24240964
hemicellulosic complexes from the cell walls of runner bean (phaseolus coccineus).	the 1 m-koh extract from the depectinated cell walls of parenchymatous tissues of mature runner bean (phaseolus coccineus) on neutralization, dialysis and concentration gave insoluble (hemicellulose a) and soluble (hemicellulose b) carbohydrate complexes in the weight ratio 2:1. both fractions contained polysaccharide, protein and polyphenolic material. the structural features of the carbohydrates were examined by methylation analysis. hemicellulose a contained mainly pectic arabinogalactan, wit ...	1985	4004776
mechanics of circadian pulvini movements in phaseolus coccineus l. : shape and arrangement of motor cells, micellation of motor cell walls, and bulk moduli of extensibility ([formula: see text]).	the circadian movement of the lamina of primary leaves of phaseolus coccineus l. is mediated by antagonistic changes in the length of the extensor and flexor cells of the laminar pulvinus. the cortex of the pulvinus is a concentric structure composed of hexagonal disc-like cells, arranged in longitudinal rows around the central stele. observations with polarization optics indicate that the cellulose microfibrils are oriented in a hoop-like fashion in the longitudinal walls of the motor cells. th ...	1985	24249410
osmotica, dimethyl sulfoxide, parahydroxymercuribenzoate, and cyanide change the period of the circadian clock in the pulvini of phaseolus coccineus l.	several substances change the period length of the circadian oscillator in the laminar pulvinus of phaseolus coccineus l. if offered continuously via the transpiration stream to the isolated leaves in continuous light and constant temperature. the osmotica mannitol and peg 6000 (polyethylene glycol) lengthen the circadian period from 27.9 hr (control) to about 29.5 hr. the dose-response of both substances reveal saturation curves. dmso (dimethyl sulfoxide) lengthens the circadian period much mor ...	1985	3939700
assignment of lectins specific for d-galactose or n-acetyl-d-galactosamine to two groups, based on their circular dichroism.	the circular dichroism (cd) spectra of thirteen lectins, most being specific for d-galactose or n-acetyl-d-galactosamine, were compared. two groupings are proposed on the basis of the cd in the near-ultraviolet region. group one comprises the lectins from arachis hypogaea, glycine max, phaseolus coccineus, p. lunatus, p. vulgaris, and vicia villosa, and group two comprises lectins from caragana arborescens, cytisus sessilifolius, dolichos biflorus, griffonia simplicifolia, and wisteria floribund ...	1985	4016573
gibberellins in suspensors of phaseolus coccineus l. seeds.	analysis of extracts from 6300 (1.2 grams fresh weight) phaseolus coccineus suspensors by combined gas chromatography-mass spectrometry has demonstrated the presence of five c(19)-gibberellins, ga(1), ga(4), ga(5), ga(6), ga(8), and one c(20)-ga, ga(44). the major gas present were ga(1) and ga(8). data are discussed in relation to previous results obtained in p. coccineus seed as well as in the embryo-suspensor system.	1986	16665010
distribution of 5-methylcytosine-rich regions in the polytene chromosomes of phaseolus coccineus embryo suspensor as shown by the immunoperoxidase technique.	5-methylcytosine (5-mc) has been visualized in polytene chromosomes of phaseolus coccineus, scarlet bean using specific antibodies to 5-mc and the immunoperoxidase technique. the results obtained indicate that most heterochromatic regions are methylated, even though the frequency of methylation is highly variable and sometimes low. a preferential binding of anti-5-mc to centromeric heterochromatic blocks was observed. comparison between anti-5-mc binding and the results of hybridization with hig ...	1986	3533573
the effects of galactolipid depletion on the structure of a photosynthetic membrane.	the galactolipids monogalactosyldiglyceride and digalactosyldiglyceride together comprise more than 77% of the photosynthetic membrane lipids of higher plant chloroplasts. we have isolated a lipase from the chloroplasts of runner beans (phaseolus vulgaris) which is highly specific for these galactolipids. this galactolipase promotes the hydrolysis of monogalactosyldiglyceride and digalactosyldiglyceride, in the process liberating two free fatty acids into the membrane bilayer, leaving the residu ...	1986	3771638
metabolism of c-zeatin in phaseolus embryos : occurrence of o-xylosyldihydrozeatin and its ribonucleoside.	the metabolism of trans-[8-(14)c]zeatin was examined in embryos of phaseolus acutifolius a. gray p.i. 321637 and phaseolus coccineus lam. cvs scarlet runner and desiree. in both species zeatin was converted to ribosylzeatin, ribosylzeatin 5'-monophosphate, o-glucosyl-9-ribosylzeatin and the recently discovered o-xylosyl derivatives of zeatin and ribosylzeatin (turner, je, dws mok, mc mok, g shaw 1987 proc natl acad sci usa. in press). two new metabolites, identified by enzyme degradation and gas ...	1987	16665486