Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| interactions of lipoidal materials and a pyridazinone inhibitor of chloroplast development. | formation of chloroplast pigments was inhibited, and free fatty acids accumulated in mustard (brassica juncea [l.] coss.) cotyledons and in barley (hordeum vulgare l.) first leaves developed after treatment with 4-chloro-5- (dimethylamino)-2- (alpha, alpha, alpha-trifluoro-m-tolyl) -3 (2h) -pyridazinone. the inhibitor reduced the amount of fatty acids found in polar lipids (galactolipids) of barley chloroplasts and increased the amount in nonpolar lipids while having little effect on total conte ... | 1971 | 16657757 |
| [studies on the volatile components of wasabia japonica, brassica juncea and cocholearia armoracia by gas chromatography-mass spectrometry. i. determination of low mass volatile components]. | 1973 | 4738516 | |
| changes in chemical & fatty acid composition during the last phase of maturation of seeds of two indian mustard cultivars brassica juncea coss. | 1974 | 4469256 | |
| isolation and characterisation of a crystalline antithiamine factor from mustard seed, brassica juncea. | 1974 | 4828856 | |
| genetic architecture of yield and components of yield in mustard (brassica juncea (l.) czern & coss.). | seed yield and some of its primary components, viz. primary and secondary branches, siliquae per plant, seeds per siliqua and seed size, were studied in the f1 and f2 populations of five intervarietal crosses of mustard. the parental cultivars represented a fairly wide range of variation for all the characters. both additive and dominance components were important for these traits and the gene actions were dependent upon the particular cross combination involved. degrees of dominance were variab ... | 1978 | 24309762 |
| phosphatase activity in susceptible and resistant cultivars of brassica juncea inoculated with isolates of macrophomina phaseolina and sclerotinia sclerotiorum. | inoculations with isolates of macrophomina phaseolina and sclerotinia sclerotiorum resulted in a significant increase in the acid phosphatase activity of susceptible and resistant cultivars of brassica juncea, which appeared to be related to the disease reaction of different host-pathogen combinations. after an increase on the 6th day of inoculation there was usually a fall in activity on the 12th and 18th days. resistant cultivars showed very poor activity in comparison to their susceptible cou ... | 1979 | 542131 |
| lipase in lipid bodies of cotyledons of rape and mustard seedlings. | lipolytic activity was absent in the crude cotyledon extract of ungerminated rapeseed (brassica napus l. var. dwarf essex), and increased to a peak at day 4 in seedling growth, concomitant with the decrease in total lipids. about 50% of the lipase activity was recovered in the lipid bodies isolated from the cotyledon extract by flotation centrifugation. isolated lipid bodies underwent autolysis of internal triacylglycerols resulting in the release of fatty acids. after the triacylglycerols in is ... | 1983 | 6614927 |
| male sterility in indian mustard (brassica juncea (l.) coss.) - a biochemical characterization. | biochemical studies were conducted on some male sterile and their fertile f1 analogues in indian mustard. the variation in ph activity during microsporogenesis was normal, except in ms-3. male sterile anthers had deficient sugar metabolism. cytochemical analysis of sporogenous and tapetal tissue suggested an effect of sterility elements on the anabolic and catabolic fate of dna and protein during microsporogenesis. leaves of male sterile lines had a higher chl a/b ratio. leaf peroxidase activity ... | 1984 | 24258839 |
| alternaria incidence in some alloplasmic lines of indian mustard (brassica juncea (l.) coss.). | the cytoplasmic substitution lines of brassica juncea (l.) coss were evaluated for their field resistance to alternaria blight (alternaria brassicae). the euplasmic b. juncea cv. 'rlm 198' had a mesothetic reaction while alloplasmic b. juncea lines with cytoplasms of b. campestris, b. chinensis, and b. japonica were highly susceptible. b. nigra cytoplasm did not have any effect on the disease reaction of the b. juncea genome. however, the alloplasmic lines with the cytoplasm of b. napus and b. c ... | 1984 | 24258547 |
| nematospora sinecauda sp. nov., a yeast pathogen of mustard seeds. | an undescribed yeast species was recovered from oriental (brassica juncea) and yellow (b. hirta) mustard seeds. the new species most closely resembled nematospora coryli but its asci were rarely cylindrical. the asci and ascospores of n. sinecauda were smaller and the spores did not possess a whip-like appendage. during germination a sprout cell formed first on the smooth anterior surface of the spore above the median ridge. the posterior region of the spore was decorated with interrupted concen ... | 1984 | 6524909 |
| chloroplast biogenesis 49 : differences among angiosperms in the biosynthesis and accumulation of monovinyl and divinyl protochlorophyllide during photoperiodic greening. | various angiosperms differed in their monovinyl and divinyl protochlorophyllide biosynthetic capabilities during the dark and light phases of photoperiodic growth. some plant species such as cucumis sativus l., brassica juncea (l.) coss., brassica kaber (dc.) wheeler, and portulaca oleracea l. accumulated mainly divinyl protochlorophyllide at night. monocotyledonous species such as avena sativa l., hordeum vulgare l., triticum secale l., zea mays l., and some dicotyledonous species such as phase ... | 1985 | 16664351 |
| regeneration of plantlets from mesophyll protoplasts of brassica juncea (l.) czern. | isolated mesophyll protoplasts of brassica juncea (l.) czern., cv. rlm 514 upon culture in suitable growth medium, regenerated cell wall, underwent cell division and formed cellular colonies. subsequent induction of embryoid (embryogenesis) and shoot bud (organogenesis) formations in such cell masses resulted in regeneration of 186 and 42 plantlets respectively. | 1985 | 24253979 |
| isozyme studies in indian mustard (brassica juncea l.). | a comparative study of peroxidase and esterase isozymes was carried out at five developmental stages of siliqua in order to characterize twelve genotypes of indian mustard. the studies showed nearly the same number of isozyme bands at every stage for peroxidase and a varying number of isozyme bands for esterase. the appearance and disappearance of bands, along with their intensity scores, indicated the role of different isozymes at different stages of siliqua development. it has been ascertained ... | 1985 | 24254123 |
| linked epistasis for six quantitative traits in indian mustard (brassica juncea (l.) czern & coss). | gene effects, and interactions, and associations between days-to-flower initiation and maturity, number of secondary branches and siliquae per plant, and 1,000-seed weight and yield per plant were studied in a cross of indian mustard (brassica juncea (l.) czern & coss) using the parents and f1, f2, f3, b1, b2, b11, b12, b21, b22, b1s, b2s, b1f1, b2f1, b1bip, b2bip, f2p1, f2f1, and f2bip generations. a linked digenic model was adequate for all characters studied. according to this model, the main ... | 1986 | 24247541 |
| effect of spiced food on metabolic rate. | since the time of lavoisier it has been known that the ingestion of food in animals and man produces an increase in oxygen consumption. this increase in metabolic rate was originally called 'specific dynamic action' (sda) and is now widely referred to as the thermic effect (te) of food or diet-induced thermogenesis (dit) (rothwell & stock, 1981). much of the early work on the thermic effect was confined to the type and amount of food, notably the macronutrients--proteins, fats and carbohydrates. ... | 1986 | 3957721 |
| [comparison between the sinigrin content of the sinapis (brassica juncea) before and after processing]. | 1987 | 2964284 | |
| generation mean analyses for flowering and maturity in indian mustard (brassica junces (l.) czern & coss). | components of generation means were partitioned for days to flower initiation and maturity in three crosses of indian mustard (brassica juncea (l.) czern and coss) cultivars. a linked digenics model was adequate for flowering in cross ii and maturity in cross i. all three types of digenic interactions among the linked pairs of genes, additive x additive (i), additive x dominance (j) and dominance x dominance (1), contributed significantly in the inheritance of flowering in cross ii and maturity ... | 1987 | 24241115 |
| intergeneric somatic hybrid production through protoplast fusion between brassica juncea and diplotaxis muralis. | the need to transfer genetic traits from diplotaxis muralis (2n=42) to brassica juncea (2n=36), a major oil seed crop of the tropical world, was realised. since the two plant types are sexually incompatible, attempts were made to evolve parasexual hybrids as the result of protoplast fusion. protoplasts of hypocotyl-derived calli of two cultivars of b. juncea were fused with normal and γ-irradiated mesophyll protoplasts of diplotaxis muralis. regeneration of 110 plants from the fused products was ... | 1988 | 24232404 |
| demethylation of satellite dna sequences in the cell cultures of brassica juncea (l.) czern. | 1988 | 3228865 | |
| a tandemly repeated dna sequence from brassica juncea. | 1989 | 2762161 | |
| isolation and characterisation of satellite dna from brassica juncea (l.) czern. | nuclear dna isolated from hypocotyls (h), proliferating callus (pc) and differentiating callus (dc) of brassica juncea contains a satellite dna which can be resolved in actinomycin-d/cscl gradients. the satellite dna undergoes changes, when an in vitro culture is raised from hypocotyl tissue and forms a higher percentage of the genome in pc and dc than in mature differentiated tissue (hypocotyl). all the three satellite dnas are gc-rich compared to main band dnas. satellite dna of h has higher t ... | 1989 | 2620907 |
| plant regeneration from hypocotyl-derived protoplasts of brassica juncea (l.) czern and coss. | protoplasts isolated from etiolated hypocotyls of 6-day-old seedlings of brassica juncea cv rlm 198 were cultured in a modified v47 medium containing 7% mannitol, 2% sucrose, 1.0 mg/l 2,4-d, 0.1 mg/l naa and 0.4 mg/l bap, at a density of 5×10(4) protoplasts per ml of medium. cultures were incubated in the dark at 25+1°c. after 7 d of culture, cell colonies were diluted with 8p medium containing 5% mannitol and a similar hormone combination as described earlier. after 14 d, cell colonies were emb ... | 1989 | 24240468 |
| factors affecting the use of chloramphenicol acetyltransferase as a marker for brassica genetic transformation. | the cat gene which codes for the enzyme chloramphenicol acetyltransferase was found to be ineffective as a reporter gene in cells and tissues of brassica species. high levels of endogenous cat activity were found to be widespread among this genus and did not appear to be distributed in a tissue- or cell-specific manner. moreover, the presence of an inhibitor of cat activity was discovered in brassica napus and brassica juncea. this inhibitor appeared to act selectively on bacterial cat in transg ... | 1989 | 24240447 |
| saskatchewan. suspected oriental mustard seed (brassica juncea) poisoning in cattle. | 1989 | 17423378 | |
| effects of ddt on the growth of crop plants. | the effects of ddt on the germination and growth of plants were studied using many crop species. of the species tested, oil-rich seeds of plants, such as peanut (arachis hypogaea) and mustard (brassica juncea), were more prone to ddt induced inhibition of germination and subsequent plant growth than cereals, pulses and fibre crops, like rice (oryza sativa), barley (hordeum vulgare), mung bean vigna radiata), pigeon pea (cajanus cajan) and cotton (gossypium hirsutum). studies with (14)c labelled ... | 1989 | 15092369 |
| virulence of agrobacterium tumefaciens strains with brassica napus and brassica juncea. | brassica napus and brassica juncea were infected with a number of agrobacterium tumefaciens strains. tumourigenesis was very rapid and extremely efficient on b. juncea with all but one of the strains. tumourigenesis on b. napus varied widely. it was very efficient with the nopaline strains, was reduced with the succinamopine strain a281 and was very weak with the octopine strains. the latter observation was confirmed with six different b. napus rapeseed cultivars. the selectivity was due to diff ... | 1989 | 24233232 |
| purification of adenine phosphoribosyltransferase from brassica juncea. | adenine phosphoribosyltransferase was purified from brassica juncea leaves approximately 4000-fold, to homogeneity. the native enzyme is a homodimer, with a mr of 54,000. the purification involved (nh4)2so4 fractionation, differential ultracentrifugation, and anion-exchange, hydrophobic, dye-ligand, and affinity chromatography. the purified enzyme has a ph optimum of 9.15 and a temperature optimum of 60 degrees c. activity of the enzyme is stimulated by mg2+ and is inhibited by sulfhydryl reagen ... | 1990 | 2275559 |
| glucosinolate levels in cotyledons of mustard,brassica juncea l. and rape,b. napus l. do not determine feeding rates of flea beetle,phyllotreta cruciferae (goeze). | sinigrin (allyl glucosinolate), the major glucosinolate in the cotyledons ofbrassica juncea cv. cutlass, occurred in the highest concentration and amount at seedling emergence and declined during growth. glucobrassicin (3-indolylmethyl glucosinolate), the major glucosinolate in the cotyledons ofb. napus cv. westar, occurred in the lowest concentration and amount at seedling emergence. the amount of glucobrassicin per cotyledon pair increased about fourfold during 14 days of growth, but its conce ... | 1990 | 24264326 |
| "erussica", the intergeneric fertile somatic hybrid developed through protoplast fusion between eruca sativa lam. and brassica juncea (l.) czern. | hypocotyl calli-derived protoplasts of two cultivars of brassica juncea (2n=36), a major oil-seed crop, were fused with normal as well as γ-irradiated mesophyll protoplasts of eruca sativa (2n=22). the irradiation of the eruca fusion partner increased the plating efficiency as well as the morphogenic potentiality of the fusion products over the normal fusion. fertile plants could be regenerated from such fusion products. analysis of 63 out of 181 plants regenerated showed that, indeed, 11 somati ... | 1990 | 24226463 |
| proline enhances primary photochemical activities in isolated thylakoid membranes of brassica juncea by arresting photoinhibitory damage. | the presence of l-proline in the reaction mixture enhances the photosystem ii (h2o----dcpip) and whole chain (h2o----mv) catalysed electron transport activities of thylakoids isolated from the cotyledonary leaves of brassica juncea seedlings raised in the absence and the presence of nacl. the extent of stimulation in activities was higher in the thylakoids of nacl raised plants than the controls. the extent of proline mediated stimulation was seen even in the presence of uncoupler nh4cl suggesti ... | 1991 | 1764073 |
| production of sodium-chloride-tolerant brassica juncea plants by in vitro selection at the somatic embryo level. | somatic embryos, developed from hypocotyl segments of light-grown seedlings of brassica juncea cv rlm198, were subjected to selection at varying concentrations of sodium chloride (nacl). plants were developed from proliferated somatic embryos selected on nacl-containing medium. the selections were characterized for salt tolerance, esterase isozyme pattern, and proline accumulation. it has been found that: (i) selected tolerant lines showed better root growth, shoot growth, and fresh weight accum ... | 1991 | 24202363 |
| molecular systematics of brassica and allied genera (subtribe brassicinae, brassiceae) -chloroplast genome and cytodeme congruence. | chloroplast dna restriction sites for 20 endonucleases were mapped using cpdna probes from brassica juncea and site variation was surveyed in 33 diploid taxa of the subtribe brassicinae. a total of 419 mutations was observed, including both site (i.e., gain/ loss) and fragment length (i.e., insertions or deletions); 221 (53%) mutations showed variation at the interspecific level. phylogenetic analysis indicated a clear division of the subtribe into two ancient evolutionary lineages. these were ( ... | 1991 | 24212864 |
| purification and partial characterization of glyoxalase i from a higher plant brassica juncea. | the glyoxalase i was purified from brassica juncea by affinity chromatography on s-hexyl gsh sepharose 4b. homogeneity of the protein was confirmed electrophoretically by a silver stained gel. activity staining on a native starch gel also showed a single band. the effect of glutathione, methylglyoxal, and ph on enzyme kinetics was studied. magnesium was found to stimulate the enzyme activity. | 1991 | 2037046 |
| proline status of genetically stable salt-tolerant brassica juncea l. somaclones and their parent cv. prakash. | three brassica juncea l. somaclones (sr-1, -2 and -3) selected in vitro for nacl-tolerance, non-selected somacone (cp-5) and parent cv. 'prakash' were characterized for their free proline contents in the absence of stress and as a function of increasing salt stress. in the r0 generation, 'sr-3' somaclone had ca. three times higher free proline as compared to parent 'prakash' and other somaclones. somaclone, sr-1, turned out to be sterile. the other somaclones were carried forward to the r2 gener ... | 1991 | 24213693 |
| interspecific hybridization between brassica juncea and b. spinescens through protoplast fusion. | hypocotyl derived protoplasts of b. juncea cv. rlm-198 were fused with mesophyll protoplasts of b. spinescens using polyethylene glycol to produce interspecific hybrids. fusion products could be microscopically identified by characteristics of the protoplasts of both parents in the hybrid cells; they are colourless and vacuolated like the hypocotyl protoplasts and possess chloroplasts of the mesophyll protoplasts. the heterokaryotic fusion frequency was around 5%. however, the frequency of calli ... | 1991 | 24213667 |
| gene transfer in plants of brassica juncea using agrobacterium tumefaciens-mediated transformation. | an efficient system for gene transfer into plants of brassica juncea var. india mustard, mediated by agrobacterium tumefaciens. was developed through the manipulation of the culture medium and the use of the appropriate agrobacterium strain. high frequency shoot regeneration (90-100%) was obtained from hypocotyl explants grown on medium containing 0.9% agarose, 3.3 mg/l agno3 and 0.5-2 mg/l ba in combination with 0.01-0.05 mg/l 2,4-d or 0.1-1 mg/l naa. of all the agrobacterium strains tested, a. ... | 1991 | 24221663 |
| isolation and characterization of a major allergen from oriental mustard seeds, braji. | a 2 s albumin from oriental mustard (brassica juncea) seeds has been isolated and characterized as an allergen. this protein, braji, was recognized by human ige from mustard-sensitive individuals, as proved by using two different enzyme immunoassays. braji was found to be closely related to sin a i, the major allergen from yellow mustard seeds. many fractions with molecular weights ranging from 16,000 to 16,400 and with differences in charge were separated by ion-exchange chromatography. they ex ... | 1991 | 1804799 |
| brassica juncea produces a phytochelatin-cadmium-sulfide complex. | phytochelatins (pcs) are enzymically synthesized peptides produced in higher plants and some fungi upon exposure to heavy metals. we have examined pc production in the se-tolerant wild mustard brassica juncea and found that it produces two types of pc-cd complexes with the same characteristics as those from fission yeast schizosaccharomyces pombe, including a high molecular weight pc-cd-sulfide form. | 1992 | 16669006 |
| the influence of plant architecture on the foraging efficiencies of a suite of ladybird beetles feeding on aphids. | by manipulating plant variety and predator species, we investigated the interactions of plant and predator traits in determining predation effectiveness. the predators were all coccinellid adults (hippodamia convergens, hippodamia variegata, coccinella apunctata, and coccinella septempunctata) and the prey were cabbage aphids (brevicoryne brassicae). foraging behavior of the four predators was observed on four crucifers that differed widely in their structures and surface textures (brassica oler ... | 1992 | 28312606 |
| plant regeneration from stem cortex expiant and protoplast cultures of brassica juncea (mustard). | plant regeneration from stem cortex explants of 13 genotypes of brassica juncea was assessed. regeneration was strongly affected by genotype, as up to 50.6 shoots were produced per 100 calli of the most responsive line (blaze), whereas no shoots were obtained from less responsive lines (zeml, vniimk351). blaze was chosen for b. juncea stem cortex protoplast isolation. after one week of culture, 11-14% of the cells had divided, and about 0.002% produced 1-2 mm colonies within 6 weeks. up to 7% of ... | 1992 | 24213363 |
| somatic hybridization between brassica juncea and moricandia arvensis by protoplast fusion. | intergeneric somatic hybrids have been produced between brassica juncea (2n=36, aabb) cv. rlm-198 and moricandia arvensis (2n=28, mm) by protoplast fusion. hypocotyl protoplasts of b. juncea were fused with mesophyll protoplasts of m. arvensis using polyethylene glycol. fusion frequency, estimated on the basis of differential morphological characterstics of parental protoplasts was about 5%. of the 156 calli obtained, four calli produced shoots intermediate in morphology between the parents. hyb ... | 1992 | 24203148 |
| isolation and sequence analysis of a cdna clone encoding ethylene-forming enzyme in brassica juncea (l.) czern & coss. | 1992 | 1623202 | |
| production and characterization of intergeneric somatic hybrids of trachystoma ballii and brassica juncea. | intergeneric somatic hybrids, trachystoma ballii (2n=16)+b. juncea (2n=38), were obtained by fusing mesophyll protoplasts of t. ballii and hypocotyl protoplasts of b. juncea using polyethylene glycol. the heterokaryotic fusion frequency was around 23%. plants were regenerated from 10 out of 2×10(4) calli of which four were hybrids. hybrids were intermediate between the parents in general morphology. however, in some characters one of the parents dominated. all the plants were symmetric in their ... | 1992 | 24213492 |
| aflatoxin contamination in field mustard (brassica juncea) cultivars. | 7 varieties of mustards were cultivated in statistically designed field during 1988 through 1989 and 1989 through 1990 crops in order to ascertain the resistant or less susceptible varieties for aflatoxin elaboration. under field condition, none of the varieties of mustard was found to be resistant. maximum aflatoxin contamination was observed in the late planting time (15 th november) crops. insect (lipaphis erysime) incidence had significant negative correlation (r=-0.9354) with the yield (q/h ... | 1992 | 23605939 |
| cloning and sequencing of 5' flanking sequence from the gene encoding 2s storage protein, from two brassica species. | using oligodeoxyribonucleotide primers and the polymerase chain reaction, we have cloned and sequenced about 1.2 kb of upstream sequences from two members of the 2s seed storage protein-encoding gene family from brassica juncea and b. oleracea. the two sequences bear more than 90% homology and have characteristic seed-specific promoter motifs. the high degree of sequence conservation indicates that this napin-encoding gene family evolved earlier than the divergence of the three primary brassica ... | 1993 | 8224919 |
| nucleotide sequence of a cdna clone encoding 1-aminocyclopropane-1-carboxylate synthase in mustard (brassica juncea [l.] czern & coss). | 1993 | 8022925 | |
| characterization of a new oriental-mustard (brassica juncea) allergen, bra j ie: detection of an allergenic epitope. | bra j ie, a major allergen from oriental-mustard (brassica juncea) seeds, has been isolated and characterized. its primary structure has been elucidated. this protein is composed of two chains (37 and 92 amino acids) linked by disulphide bridges. the amino acid sequence obtained is closely related to that previously determined for sin a i, an allergen isolated from yellow mustard (sinapis alba). a common epitope has been detected in the large chain of both bra j ie and sin a i by means of electr ... | 1993 | 7688955 |
| auxin polar transport is essential for the establishment of bilateral symmetry during early plant embryogenesis. | we used an in vitro culture system to investigate the effects of three auxin polar transport inhibitors (9-hydroxyfluorene-9-carboxylic acid, trans-cinnamic acid, and 2,3,5-triiodobenzoic acid) on the development of early globular to heart-shaped stage embryos of indian mustard (brassica juncea) plants. although the effective concentrations vary with the different inhibitors, all of them induced the formation of fused cotyledons in globular ([less than or equal to]60 [mu]m) but not heart-shaped ... | 1993 | 12271078 |
| variation amongst brassica juncea cultivars for regeneration from hypocotyl explants and optimization of conditions for agrobacterium-mediated genetic transformation. | twelve cultivars of brassica juncea grown in different agroclimatic regions of the world were tested for their ability to regenerate in vitro from hypocotyl explants and, accordingly, were divided into three groups. one group of cultivars regenerated on ms medium supplemented with 2,4-d, bap and with naa, bap combinations; another group regenerated only on ms with 2,4-d, bap; and the third group showed very low regeneration on both of these combinations. inclusion of silver nitrate in the medium ... | 1993 | 24197353 |
| a continuous culture system of direct somatic embryogenesis in microspore-derived embryos of brassica juncea. | an efficient culture system has been developed for repeated cycles of somatic embryogenesis in microspore-derived embryos of brassica juncea without a callus phase. haploid embryos produced through anther culture showed a high propensity for direct production of somatic embryos in response to 2 mgl(-1) ba and 0.1 mgl(-1) naa. the embryogenic cultures which comprised the elongated embryonal axis of microspore-derived embryos when explanted and grown on the medium of same composition produced a la ... | 1993 | 24197160 |
| lipids, proteins, and structure of seed oil bodies from diverse species. | oil bodies isolated from the mature seeds of rape (brassica napus l.), mustard (brassica juncea l.), cotton (gossypium hirsutum l.), flax (linus usitatis simum), maize (zea mays l.), peanut (arachis hypogaea l.), and sesame (sesamum indicum l.) had average diameters that were different but within a narrow range (0.6-2.0 [mu]m), as measured from electron micrographs of serial sections. their contents of triacylglycerols (tag), phospholipids, and proteins (oleosins) were correlated with their size ... | 1993 | 12231682 |
| identification and quantification of the n-acetylcysteine conjugate of allyl isothiocyanate in human urine after ingestion of mustard. | allyl isothiocyanate (aitc) is a constituent of cruciferous vegetables. it occurs widely in the human diet as a natural ingredient or food additive. aitc possesses numerous biochemical and physiological activities. it is cytotoxic and tumorigenic at high doses and also is a modulator of enzymes involved in metabolism of xenobiotics, including carcinogens. it is plausible that the wide consumption of dietary aitc may have profound effects on human health. to facilitate investigations of the effec ... | 1994 | 8000299 |
| immunopurification and immunocharacterization of the glucosinolate biosynthetic enzyme thiohydroximate s-glucosyltransferase. | preparing homogeneous udp-glucose:thiohydroximate s-glucosyltransferase (s-gt), the penultimate biosynthetic enzyme of glucosinolates, by standard chromatographic methods has yielded too little protein for adequate purity evaluation, identity verification, and structural analysis. the low yields were apparently due to low abundance in source tissues, aggravated by enzyme instability. here we describe an immunological method for purification of workable quantities from florets of brassica olerace ... | 1994 | 12232212 |
| genomic organization of the canrep repetitive dna in brassica juncea. | canrep is a heterogeneous, tandemly repeated, 176 bp nucleotide sequence that contains a single hind iii site and is present in high copy numbers in the genomes of many brassica species. complete clusters of repeats of this dna were cloned from the nuclear dna of brassica juncea. restriction-fragment dimers and higher multimers of the 176 bp sequence have arisen by mutations within the hind iii recognition sequence. adjacent repeats from within the same cluster usually have different nucleotide ... | 1994 | 7999997 |
| ethylene production during development of mustard (brassica juncea) and canola (brassica napus) seed. | an open, continuous flow system was used to investigate ethylene production during degreening of maturing seed of mustard (brassica juncea cv cutlass and cv lethbridge 22a) and canola (brassica napus cv westar and cv alto). isolated mustard seed evolved higher amounts of ethylene than those of canola, and this was particularly evident both early in embryogeny and later during the desiccation phase of seed maturation. the silique walls produced negligible amounts of ethylene in both species. the ... | 1994 | 12232353 |
| lipid components of mustard seeds (brassica juncea l.) as influenced by cadmium levels. | in a pot experiment the soil application of different levels of cd2+ (0, 10, 20, 30, 40 and 60 micrograms g-1 soil) affected the lipid components of mustard seeds (brassica juncea l. cv. rh-30) markedly. total lipids declined with the cd2+ levels regularly while phospho and glycolipids increased only at higher levels. fatty acids profile of total, neutral and polar lipid fractions were affected considerably. erucic acid in total and neutral lipids was observed to increase while it decreased in p ... | 1994 | 7855087 |
| cdna clones for salt-inducible genes from mustard (brassica juncea). | treatment of a salt tolerant variety of mustard (brassica juncea cv rh30), with 0.2m nacl for 96 hr was found to induce synthesis of three new polypeptides and accumulation of proline. a cdna library from mrnas derived from salt stressed plants was constructed in the expression vector lambda gt11. screening by differential hybridization and by salt stress specific antibodies gave two clones msc1 and msc2 (mustard salt clone) respectively. msc1 hybridized to transcripts from salt stressed mustard ... | 1994 | 8002016 |
| potential use of random amplified polymorphic dna (rapd) technique to study the genetic diversity in indian mustard (brassica juncea) and its relationship to heterosis. | rapd assays were performed, using 34 arbitrary decamer oligonucleotide primers and six combinations of two primers, to detect inherent variations and genetic relationships among 12 indian and 11 exotic b. juncea genotypes. of 595 amplification products identified, 500 of them were polymorphic across all genotypes. a low level of genetic variability was detected among the indian genotypes, while considerable polymorphism was present among the exotic ones. based on the pair-wise comparisons of amp ... | 1994 | 24185891 |
| mechanisms of cadmium mobility and accumulation in indian mustard. | indian mustard (brassica juncea l.), a high biomass crop plant, accumulated substantial amounts of cadmium, with bioaccumulation coefficients (concentration of cd in dry plant tissue/concentration in solution) of up to 1100 in shoots and 6700 in roots at nonphytotoxic concentrations of cd (0.1 [mu]g/ml) in solution. this was associated with a rapid accumulation of phytochelatins in the root, where the majority of the cd was coordinated with sulfur ligands, probably as a cd-s4 complex, as demonst ... | 1995 | 12228679 |
| somatic hybrids between brassica juncea (l). czern. and diplotaxis harra (forsk.) boiss and the generation of backcross progenies. | an attempt to transfer genes from droughttolerant diplotaxis harra, a wild relative of brassica species, to an elite oil-yielding cultivar, b-85, of mustard (brassica juncea) was made through protoplast fusion, as the two plant systems are sexually incompatible. by following the standard protocol for peg-mediated protoplast fusion followed by high ph, high ca(++), dmso treatment and appropriate cell-culture technique, 16 presumptive somatic hybrid plants could be regenerated. chromosomal analysi ... | 1995 | 24170013 |
| dna fingerprinting of brassica juncea cultivars using microsatellite probes. | the genetic variability in the brassica juncea cultivars was detected by employing in-gel hybridization of restricted dna to simple repetitive sequences such as (gata)4, (gaca)4 and (cac)5. the most informative probe/enzyme combination was (gata)4/ecori, yielding highly polymorphic fingerprint patterns for the b. juncea cultivars. this technique was found to be dependable for establishing the variety specific patterns for most of the cultivars studied, a prerequisite for germplasm preservation. ... | 1995 | 8582366 |
| transfer of ogu cytoplasmic male sterility to brassica juncea and improvement of the male sterile line through somatic cell fusion. | male sterility conferred by ogu cytoplasm of raphanus sativus has been transferred to brassica juncea cv 'rlm 198' from male-sterile b. napus through repeated backcrossing and selection. the male-sterile b. juncea is, however, highly chlorotic and late. it has low female (seed) fertility and small contorted pods. to rectify these defects, protoplasts of the male sterile were fused with normal 'rlm 198' (green, self fertile). four dark green, completely male-sterile plants were obtained and ident ... | 1995 | 24169844 |
| diplotaxis catholica + brassica juncea somatic hybrids: molecular and cytogenetic characterization. | intergeneric somatic hybrids diplotaxis catholica (2n=18) + brassica juncea (2n=36) were produced by fusing mesophyll protoplasts of the former and hypocotyl protoplasts of the latter using polyethylene glycol. out of 52 somatic embryos, 24 produced plants of intermediate morphology. cytological analysis of 16 plants indicated that 15 were symmetric hybrids carrying 54 chromosomes, the sum of the parental chromosome numbers. one hybrid was asymmetric with 45 chromosomes. nuclear hybridity of fiv ... | 1995 | 24185604 |
| cloning and nucleotide sequence of a cdna encoding s-adenosyl-l-methionine synthetase from mustard (brassica juncea [l.] czern & coss). | 1995 | 7716233 | |
| a cdna clone encoding brassica calmodulin. | a 834 bp cdna encoding calmodulin (cam) has been isolated from brassica juncea. on northern analysis this cdna hybridises this cdna to mrnas of about 0.9 kb in leaf, silique and peduncle. genomic southern analysis indicates the presence of a cam multigene family in brassica juncea. comparison of the predicted amino acid sequence of brassica cam with that of arabidopsis cam acam-2 and acam-3 showed 100% homology, which is not unusual, since both plants belong to the family cruciferae. in situ hyb ... | 1995 | 7888630 |
| haematological & histological studies after curry leaf (murraya koenigii) & mustard (brassica juncea) feeding in rats. | whole curry leaf and mustard fed to rats at doses equal to normal human intake did not cause any adverse effect on food efficiency ratio (fer), red blood cell count (rbc), white blood cells (wbc), total count, differential counts or on the levels of blood constituents, like serum electrolytes, blood urea, haemoglobin, total serum protein, albumin-globulin ratio, fibrin level, glycosylated haemoglobin and the activity of glutamic oxaloacetic transaminase (got), glutamic pyruvic transaminase (gpt) ... | 1995 | 8543364 |
| induction of tumours by indian dietary constituents. | fishes and vegetables are preserved by salting and sundrying; and later fried in oil and consumed. such preparations have been found to contain polycyclic aromatic hydrocarbons, and are genotoxic and mutagenic. the potential carcinogenic effects of these and other dietary items have been studied by oral feeding to swiss mice at 100 mg/animal/day for 12 months; and observing upto two years. when so prepared and tested, chillies (capsicum annum l.) produce adenocarcinomas in the abdomen in 35% of ... | 1995 | 8772809 |
| degradation of sinigrin by lactobacillus agilis strain r16. | forty-two lactobacilli were screened for their potential to degrade glucosinolate sinigrin. one of them, strain r16, demonstrated a high level of sinigrin degradation; it was identified as lactobacillus agilis. the sinigrin degrading activity of l. agilis r16 could only be demonstrated when intact cells were used. the products of sinigrin degradation are allyl-isothiocyanate (aitc) and glucose (which is further fermented to dl-lactic acid), suggesting that myrosinase activity is involved. the ac ... | 1995 | 7577359 |
| immunological evidence for accumulation of two high-molecular-weight (104 and 90 kda) hsps in response to different stresses in rice and in response to high temperature stress in diverse plant genera. | rice seedlings accumulate stainable amounts of the 104 and 90 kda polypeptides in response to high temperature stress. we have purified and raised highly specific polyclonal antisera against both of these polypeptides. in western blotting experiments, we find that these proteins are accumulated to different extents in rice seedlings subjected to salinity (nacl), water stress, low-temperature stress and exogenous abscisic acid application. these proteins also accumulated when rice seedlings grown ... | 1995 | 7579180 |
| horizontal transfer in the phytopathogenic fungal genus leptosphaeria and host-range expansion. | all isolates examined of the phytopathogenic fungus leptosphaeria maculans that are aggressive to brassica napus and brassica rapa have a repetitive element, lmr1. horizontal transfer of lmr1 to an isolate of a closely related species of leptosphaeria correlates with the expansion of the host range of this isolate to include brassica juncea. | 1995 | 7627459 |
| hypoglycemic action of murraya koenigii (curry leaf) and brassica juncea (mustard): mechanism of action. | effect of murraya koenigii and brassica juncea on carbohydrate metabolism has been studied using rats as experimental animals. both showed significant hypoglycemic action. there was increase in the concentration of hepatic glycogen and glycogenesis, as evident from the increased activity of glycogen synthetase, and decrease in glycogenolysis and gluconeogenesis as evident from the decreased activity of glycogen phosphorylase and gluconeogenic enzymes. | 1995 | 7642200 |
| proline accumulates in plants exposed to uv radiation and protects them against uv induced peroxidation. | proline accumulated in the shoots of seedlings of rice (oryza sativa), mustard (brassica juncea) and mung bean (vigna radiata) exposed to uv radiations. the level of proline in the seedlings increased significantly with increase in uv exposure time. the production of malondialdehyde (an indice of lipid peroxidation) was also higher in the shoots of seedlings exposed to uv radiation as compared to controls, suggesting that uv radiations promote lipid peroxidation. the extent of uv radiation promo ... | 1995 | 7726821 |
| biochemical response in rats to the addition of curry leaf (murraya koenigii) and mustard seeds (brassica juncea) to the diet. | three groups with 12 weanling male albino rats were group fed for 90 days on a standard laboratory rat diet plus 20% coconut oil either without a supplement or with the addition of 10% curry leaf or 10% mustard seeds. feed was offered at a level of 10% body weight. at the end of the trial, measurements were made on the total serum cholesterol, high density lipoproteins, low density lipoproteins, and very low density lipoproteins fractions, release of lipoproteins into the circulation, lecithin c ... | 1996 | 8983055 |
| role of murraya koenigii (curry leaf) and brassica juncea (mustard) in lipid peroxidation. | the status of lipid peroxidation was investigated in rats fed m. koenigii (curry leaf) and b. juncea (mustard). concentration of malondialdehyde showed a significant decrease, while hydroperoxides and conjugated dienes were significantly increased in liver and heart of both the experimental groups. sod and catalase activity was found to be increased in liver and heart of both the spices administered groups. glutathione levels in liver, heart and kidney were lowered in rats administered these spi ... | 1996 | 9062811 |
| murraya koenigii and brassica juncea--alterations on lipid profile in 1-2 dimethyl hydrazine induced colon carcinogenesis. | the influence of the two commonly used spices murraya koenigii (curry leaf) leaf and brassica juncea (mustard) seeds on the levels of lipids, fecal bile acids and neutral sterols was studied in rats administered 1,2-dimethyl hydrazine (1,2 dmh). the levels of cholesterol and phospholipids decreased in the experimental groups when compared with the control. the cholesterol phospholipid ratio showed an elevated level in the dmh treated control compared with the species group. bile acids and neutra ... | 1996 | 9157071 |
| evolutionary consequences of simulated global change: genetic adaptation or adaptive phenotypic plasticity. | during the next century, natural and agricultural systems might need to adjust to a rapid increase in atmospheric co2 concentration and global temperature. evolution of genotypes adapted to this global change could play a central role in plants' response. the main purpose of this study was to determine the relative importance of phenotypic and genotypic responses of plants to global change. to do so, we selected two populations of the short-lived brassica juncea, one under ambient conditions and ... | 1996 | 28307802 |
| inheritance and expression patterns of bn28, a low temperature induced gene in brassica napus, throughout the brassicaceae. | molecular genetics is becoming an important tool in the breeding and selection of agronomically important traits. bn28 is a low temperature induced gene in brassicaceae species. pcr and southern blot analysis indicate that bn28 is polymorphic in the three diploid genomes: brassica rapa (aa), brassica nigra (bb), and brassica oleracea (cc). of the allotetraploids, brassica napus (aacc) is the only species to have inherited homologous genes from both parental genomes. brassica juncea (aabb) and br ... | 1996 | 18469930 |
| the genetics of adult-plant blackleg (leptosphaeria maculans) resistance from brassica juncea in b. napus. | the genetic control of adult-plant blackleg (leptosphaeria maculans) resistance in a brassica napus line (579no48-109-dg-1589), designated "r13" possessing brassica juncea-like resistance (jr), was elucidated by the analysis of segregation ratios in f2 and f3 populations from a cross between "r13" and the highly blackleg-susceptible b. napus cultivar "tower". the f2 segregration ratios were bimodal, demonstrating that blackleg resistance in "r13" was controlled by major genes. analysis of the se ... | 1996 | 24166261 |
| absorption and degradation of metalaxyl in mustard plant (brassica juncea). | absorption and degradation of metalaxyl were studied in mustard (brassica juncea) plants after application as a seed dresser, a foliar spray, and a combination of both under subtropical conditions in india. results indicated that absorption of metalaxyl increased up to 30 days when it was applied as a seed dresser; thereafter, it started declining and was not detectable after 60 days of sowing. the maximum residues (average, 9.03 ppm) of metalaxyl were found after 1 day of spraying. the dissipat ... | 1997 | 9262951 |
| selenium-induced growth reduction in brassica land races considered for phytoremediation. | brassica species considered for use in selenium (se) phytoremediation need to accumulate large amounts of se to be successful. retarded plant growth and impaired protein synthesis are common symptoms for plants grown under seleniferous soils. selenium accumulation by different land races of brassica juncea (l.) czern and coss and one land race of brassica carinata was investigated in se-enriched water and soil cultures containing 2 mg se kg-1. effects of se concentration in the root environment ... | 1997 | 9143457 |
| in seedlings of the heavy metal accumulator brassica juncea cu2+ differentially affects transcript amounts for gamma-glutamylcysteine synthetase (gamma-ecs) and metallothionein (mt2). | glutathione (gsh) is the precursor of the phytochelatins (pc), which in plants and fungi are involved in heavy metal sequestration. the regulatory enzyme gamma-glutamylcysteine synthetase (gamma-ecs) catalyzes the first step in gsh biosynthesis. for the heavy metal accumulator brassica juncea l. a partial gamma-ecs cdna was cloned by rt-pcr. treatment of suspension-cultured dark grown seedlings with micromolar concentrations of cuso4 resulted in a strong increase of gamma-ecs mrna in roots and s ... | 1997 | 9119067 |
| purification and properties of phenylalanine ammonia-lyase from leaf mustard. | phenylalanine ammonia-lyase (pal, ec 4.3.1.5), the first enzyme in phenylpropanoid biosynthesis, catalyzes the elimination of ammonium ion from l-phenylalanine. in the present study, pal was purified through ammonium sulfate fractionation, deae-cellulose chromatography, sephadex g-200 chromatography, and q-sepharose chromatography from the cytosolic fraction of leaf mustard (brassica juncea var. integrifolia). it consists of 4 subunits, each having an estimated molecular weight of about 40,000 o ... | 1997 | 9509410 |
| nutritional evaluation of low glucosinolate mustard meals (brassica juncea) in broiler diets. | experiments were conducted to evaluate the nutritional value of meal derived from low glucosinolate cultivars of mustard (brassica juncea) in comparison to samples of canola meal (brassica napus, brassica rapa). samples of brassica seed (four b. juncea, one b. napus, and one b. rapa) were processed using laboratory procedures to produce oil-extracted meals, which were examined for composition (dm basis), and nutritional value for broiler chickens as judged by nutrient retention (amen, ileal prot ... | 1997 | 9276890 |
| anti-oxidant effects of curry leaf, murraya koenigii and mustard seeds, brassica juncea in rats fed with high fat diet. | status of lipid peroxidation was studied in rats induced high fat diet and some commonly used spices, viz. murraya koenigit and brassica juncea. the study revealed that these species alter the peroxidation (thiobarbituric acid reactive substances) level to a beneficial extent. histological studies also focus on modulation of hepatic functions to near normal level. | 1997 | 9315222 |
| inheritance of resistance to leptosphaeria maculans in brassica juncea. | abstract the inheritance of resistance to leptosphaeria maculans, the causal agent of black leg of crucifers, was studied in brassica juncea. three resistant accessions (um3021, um3043, and um3323) and one susceptible accession (um3132) of b. juncea were crossed in a complete diallel. parents, f(1), and f(2) progenies were evaluated for all crosses using both cotyledon and stem inoculation. cotyledon reaction was evaluated with two isolates of l. maculans, but stem reaction was evaluated with on ... | 1997 | 18945075 |
| characterization of tscl, a nonviral retroposon from arabidopsis thaliana. | we isolated by differential screening a 1.2 kb cdna from an arabidopsis thaliana ecotype columbia cdna library that is highly expressed in stem and root. in situ hybridization studies on stem sections and root sections showed that the mrna is expressed in stem sclerenchyma and root cortex, respectively. the isolation and sequence analysis of four other overlapping cdna clones from two independent a. thaliana cdna libraries confirmed that these cdnas lack a significant open reading frame that has ... | 1997 | 9426608 |
| a urinary biomarker for uptake of dietary isothiocyanates in humans. | isothiocyanates (itcs) are a family of biologically active compounds that are distributed widely in cruciferous vegetables. although studies in rodents have shown that these compounds are effective and versatile inhibitors of tumorigenesis, the role of dietary itcs in the protection against human cancers remains to be established. a prerequisite of human studies is to develop an uptake biomarker for dietary itcs. in this study, we describe a rapid high-performance liquid chromatography-based ass ... | 1998 | 9488583 |
| distribution patterns of 104 kda stress-associated protein in rice. | a 104 kda protein (sap 104) accumulates in rice seedlings in response to several abiotic stress conditions and immunological homologues of rice sap 104 have been detected in several monocot and dicot species, as also neurospora crassa, a fungus. in this report, we show that the amino acid sequence of a tryptic peptide generated from purified sap 104 bears significant homology with an atp-binding domain of the hsp 100 family proteins of arabidopsis thaliana and glycine max. it is further shown th ... | 1998 | 9700064 |
| sub-cellular immunolocalization of the glucosinolate sinigrin in seedlings of brassica juncea. | polyclonal rat antibodies were raised to a bovine serum albumin-sinigrin conjugate and used to immunolocalize sinigrin (2-propenylglucosinolate) in imbibed seeds and developing seedlings of brassica juncea. (l.) czern. sinigrin was localized to protein bodies in aleurone-like cells but shown to be absent from myrosin cells. double labelling techniques were used to co-localize both myrosinase (beta-thioglucoside glucohydrolase, ec 3.2.3.1) and sinigrin. myrosin grains were labelled only with the ... | 1998 | 9763706 |
| biochemical and immunochemical characterization of brassica juncea glyoxalase i. | a homogenous preparation of glyoxalase i (s-lactoylglutathione-lyase, ec 4.4.1.5) was obtained from brassica juncea seedlings. the enzyme is a heterodimer with 27,000 and 29,000 m(r) subunits and native m(r) of 56,000. the circular dichroic spectra of the protein showed characteristics of a distinctly helical protein, and magnesium affected the secondary structure. it is a zinc metalloenzyme. amino acid modification studies suggested the involvement of histidine residues in catalysis. apo-glyoxa ... | 1998 | 9887525 |
| production of 2,5-dioxopiperazine by a new isolate type of the blackleg fungus phoma lingam. | new isolates of the blackleg fungus [phoma lingam, perfect stage leptosphaeria maculans (desm.) ces. et de not.], virulent to the traditionally resistant brown mustard (brassica juncea), produce l-valyl-l-tryptophan anhydride, a 2,5-dioxopiperazine different from those produced by blackleg isolates virulent to canola (b. napus and b. rapa). | 1998 | 11711067 |
| ric c 1 and ric c 3, the allergenic 2s albumin storage proteins of ricinus communis: complete primary structures and phylogenetic relationships. | the 2s albumin storage protein of ricinus communis consists of the two heterodimeric proteins ric c 1 and ric c 3 each of which is composed of a small and a large subunit linked together by disulphide bridges. the complete primary structures of both heterodimeric proteins were determined by enzymatic degradation and automated edman degradation. the sequences of all four chains correspond to the known cdna sequence of the gene of a presumed precursor molecule and to the previously determined part ... | 1998 | 9430499 |
| recombination between chloroplast genomes of trachystoma ballii and brassica juncea following protoplast fusion. | we document here the presence of a recombinant plastome in a cytoplasmic male sterile (cms) line of brassica juncea developed from the somatic hybrid trachystoma ballii + b. juncea. restriction endonuclease digestion of the chloroplast (cp) dna has revealed that the recombinant plastome gives rise to novel fragments in addition to the parent-specific fragments. analysis of the 16s rrna region by southern hybridization shows no variation between b. juncea, t. ballii and the cms line. the rbcl gen ... | 1998 | 9870700 |
| proline suppresses rubisco activity in higher plants. | seedlings of brassica juncea, sesbania sesban, and oryza sativa exposed to salt stress accumulated proline to levels as high as 4- to 20-fold over those of controls. because chloroplasts are the major site for synthesis of stress induced proline accumulation, in vitro studies were conducted to see how the over-accumulation of this solute influences the activity of the major chloroplastic enzyme ribulose 1,5-bis-phosphate carboxylase (rubisco) purified from the above plant species. surprisingly, ... | 1998 | 9826546 |
| rate-limiting steps in selenium assimilation and volatilization by indian mustard | se can be accumulated by plants and volatilized to dimethylselenide, providing an attractive technology for se phytoremediation. to determine the rate-limiting steps in se volatilization from selenate and selenite, time- and concentration-dependent kinetics of se accumulation and volatilization were studied in indian mustard (brassica juncea). time-dependent kinetic studies showed that selenate was taken up 2-fold faster than selenite. selenate was rapidly translocated to the shoot, away from th ... | 1998 | 9701603 |
| growth responses of indian mustard [brassica juncea (l.) czern.] and its phytoextraction of lead from a contaminated soil. | 1998 | 9657828 | |
| the role of edta in lead transport and accumulation by indian mustard | indian mustard (brassica juncea) plants exposed to pb and edta in hydroponic solution were able to accumulate up to 55 mmol kg-1 pb in dry shoot tissue (1.1% [w/w]). this represents a 75-fold concentration of pb in shoot tissue over that in solution. a threshold concentration of edta (0.25 mm) was found to be required to stimulate this dramatic accumulation of both pb and edta in shoots. below this threshold concentration, edta also accumulated in shoots but at a reduced rate. direct measurement ... | 1998 | 9625697 |
| cdna cloning and expression analysis of genes encoding gsh synthesis in roots of the heavy-metal accumulator brassica juncea l.: evidence for cd-induction of a putative mitochondrial gamma-glutamylcysteine synthetase isoform. | in roots of brassica juncea l. cadmium (cd) exposure (25 microm) induces a massive formation of phytochelatins (pcs), which is accompanied by an only moderate decrease (-20%) of the putative pc precursor glutathione (gsh). as pc formation in roots could be the result of local gsh de novo synthesis and/or depend on gsh import from the shoot, we have analyzed the expression of the enzymes involved in gsh synthesis in the root, namely oas(thiol)lyase (oas-tl; catalysing the last step in cys biosynt ... | 1998 | 9620267 |
| auxin-induced developmental patterns in brassica juncea embryos. | to investigate the mechanism of auxin action during pattern formation in dicot embryos, we tested the effects of the natural auxin indole-3-acetic acid (iaa), the auxin transport inhibitor n-(1-naphthyl)thalamic acid (npa) and the antiauxin p-chlorophenoxyisobutyric acid (pcib). in vitro treatments of isolated zygotic brassica juncea embryos with these substances led to a wide range of morphogenetic alterations. treatment of globular embryos with exogenous auxin (10-40 microm) either completely ... | 1998 | 9449670 |
| biochemical changes associated with brassica juncea seed development. iv. acid and alkaline phosphatases. | changes in acid and alkaline phosphatase activities in cytoplasmic and wall-bound fractions of developing mustard (brassica juncea) seed were studied. growth was measured by seed dry weight and water content. seed dry weight data were fitted to a cubic polynomial equation. seed water content and dry matter accumulation was significantly correlated. cytoplasmic acid and alkaline phosphatase activities were substantially less in the cytoplasmic fraction than the wall-bound fraction. wall-bound aci ... | 1999 | 10552135 |