Publications

cytoprotective effects of pumpkin (cucurbita moschata) fruit extract against oxidative stress and carbonyl stress.	background diabetes mellitus is a chronic endocrine disorder that is associated with significant mortality and morbidity due to microvascular and macrovascular complications. diabetes complications accompanied with oxidative stress and carbonyl stress in different organs of human body because of the increased generation of free radicals and impaired antioxidant defense systems. in the meantime, reactive oxygen species (ros) and reactive carbonyl species (rcs) have key mediatory roles in the deve ...	0	28586926
pumpkin seed (cucurbita moschata) in the treatment of acute schistosomiasis.		1960	13810019
chemical studies on cucurbita moschata duch. ii. a synthesis of cucurbitine.		1961	13918398
chemical studies on cucurbita moschata duch. i. the isolation and structural studies of cucurbitine, a new amino acid.		1961	13891528
metabolism of arginine by aging and 7 day old pumpkin seedlings.	the metabolism of arginine by etiolated pumpkin (cucurbita moschata) seedlings was studied over various time and age intervals by injecting arginine-u-(14)c into the cotyledons. at most, 25% of the (14)c was transported from the cotyledon to the axis tissue and the amount of this transport decreased with increasing age of the seedlings. the cotyledons of 25 day old plants contained 60% of the administered (14)c as unmetabolized arginine. little (14)c was in sugars and it appeared that arginine w ...	1969	16657070
glutamate dehydrogenase from pumpkin cotyledons: characterization and isoenzymes.	glutamate dehydrogenase from pumpkin (cucurbita moschata pior. cultivar dickinson field) cotyledons was found in both soluble and particulate fractions with the bulk of the activity in the soluble fraction. both enzymes used nad(h) and nadp(h) but nad(h) was favored. the enzymes were classified as glutamate-nad oxidoreductase, deaminating (ec 1.4.1.3). both enzymes were heat stable, had a ph optimum for reductive amination of 8.0, and were inhibited by high concentrations of nh(4) (+) or alpha-k ...	1972	16657999
globulin-specific proteolytic activity in germinating pumpkin seeds as detected by a fluorescence assay method.	the proteolytic activities of alpha-chymotrypsin, trypsin, pepsin, bromelain, and an extract from germinating pumpkin seeds (cucurbita moschata) were determined by their ability to effect the release of 1-anilino-8-naphthalenesulfonate bound to internal hydrophobic sites in intact protein substrates. casein, glyceraldehyde-3-p dehydrogenase, urease, catalase, pumpkin seed globulin, and bovine serum albumin enhanced the fluorescence of 1-anilino-8-naphthalenesulfonate sufficiently to be used as p ...	1974	16659002
chemical feeding deterrent mobilized in response to insect herbivory and counteradaptation by epilachna tredecimnotata.	experimentally damaged leaves of cucurbita moschata mobilize substances to the damaged region within 40 minutes. these substances stimulate feeding by acalymma vittata (coleoptera: chrysomelidae) and inhibit feeding by epilacha tredecimnotata (coleoptera: coccinelidae). under natural conditions, epilachna cuts a circular trench in the leaf before feeding on the encircled leaf material, thus preventing mobilization of the deterrent substances to the feeding area.	1980	17747815
d-amino-acid-stimulated ethylene production in seed tissues.	ethylene production by axial and cotyledonary tissues excised from xanthium pennsylvanicum wallr. seeds was markedly (up to 5-fold) stimulated by the d-isomers of phenylalanine, valine, leucine, threonine, methionine and eithionine while the l-isomers caused no such effect. responsiveness of these seed tissues to d-methionine appeared soon after the beginning of imbibition, reached a maximum after 6-12 and 12-24 h for the axial and cotyledonary tissues, respectively, and then decreased sharply. ...	1980	24306194
effects of root applications of gibberellic acid on photosynthesis and growth in c3 and c 4 plants.	the effects of root applications of gibberellic acid (ga3) on growth and photosynthesis of 12 species of plants including c3 monocots (triticum aestivum l., wheat, hordeum vulgare l., barley and avena sativa l., oat), c3 dicots (vigna radiata l., mung bean, cucurbita moschata l., squash and capsicum annuum l., pepper), c4 monocots (zea mays l., corn, sorghum vulgare l., sorghum and panicum ramosum l., millet) and c4 dicots (amaranthus retroflexus l., pigweed, kochia scoparis l., kochia and gomph ...	1985	24442874
the regulation of gelation of phloem exudate from cucurbita fruit by dilution, glutathione, and glutathione reductase.	the average glutathione equivalent concentration in phloem exudate collected from squash fruit (cucurbita moschata [duchesne] poir. var butternut) and pumpkin fruit (cucurbita pepo [l.] var jack-o-lattern) was 1.02 and 0.60 millimolar, respectively. glutathione reductase (ec 1.6.4.2) activity in phloem exudate from squash and pumpkin fruit averaged 0.48 and 1.74 micromole nadph oxidized per minute per milliliter, respectively. protein concentrations in fruit phloem exudates averaged 67 milligram ...	1988	16666036
carotenoid composition and vitamin a value of a squash and a pumpkin from northeastern brazil.	the carotenoid composition of a squash and a pumpkin from northeastern brazil was determined. nineteen carotenoids were detected in cucurbita moschata variety "baianinha"; beta-carotene was the principal carotenoid, contributing about 74% of an average total carotenoid content of 317.8 micrograms/g. in c. maxima variety "jerimum caboclo", 11 carotenoids were found with lutein, and beta-carotene as the major pigments accounting for about 60% and 27%, respectively, of an average total carotenoid c ...	1990	2133187
characterization of the major integral protein of vacuolar membrane.	the vacuolar membrane of radish (raphanus sativus) taproot contained a large quantity of a protein of 23 kilodaltons that accounted for more than 25% of the total membrane proteins. the protein, tentatively named vm 23, was purified and characterized. vm 23 tends to aggregate at high temperature even in the presence of 1% sodium dodecyl sulfate. the apparent molecular size of vm 23 was estimated to be about 400 kilodaltons by polyacrylamide gel electrophoresis in the presence of 0.1% triton x-10 ...	1992	16668783
[chemical and biological characterization of meal and protein isolates from pumpkin seed (cucurbita moschata)].	the present study was carried out in order to check through chemical and biological analyses the nutritional characteristics of pumpkin seed, its delipidized meal and its proteic concentrate, considering its availability, nutritional potential, facility for production in poor soils and the need for new food resources. another objective was to complement the amino acid pattern of pumpkin with others protein sources for human consumption. the results obtained indicate that: raw pumpkin seed meal h ...	1992	1342182
[effects of celosia argentea and cucurbita moschata extracts on anti-dnp ige antibody production in mice].	we have already reported that the perilla frutescens extract (pfe) suppressed anti-dna ige antibody production in mice. in this study, we prepared extracts of celosia argentea l. (cae) and cucurbita moschata duch (cme), which are chinese herbal medicines like perilla frutescens, and examined the effects on anti-dnp antibody responses in mice. to examine the effects of cae & cme on primary antibody responses, cae & cme were intraperitoneally injected the day before primary immunization of dnp-ova ...	1994	8031259
butternut squash (cucurbita moschata) dermatitis.		1994	8187500
the sterols of cucurbita moschata ("calabacita") seed oil.	from the sterol fraction of seed oil from commercial cucurbita moschata dutch ("calabacita") delta 5 and delta 7 sterols having saturated and unsaturated side chain were isolated by chromatographic procedures and characterized by spectroscopic (1h and 13c-nuclear magnetic resonance, mass spectrometry) methods. the main components were identified as 24s-ethyl 5 alpha-cholesta-7,22e-dien-3 beta-ol (alpha-spinasterol); 24s-ethyl 5 alpha-cholesta-7,22e,25-trien-3 beta-ol (25-dehydrochondrillasterol) ...	1996	8934454
plant exudates trigger leaf-trenching by cabbage loopers, trichoplusia ni (noctuidae).	cabbage loopers, trichoplusia ni, cut a narrow trench across leaves of plants that release exudate, then feed distal to the trench in an area of reduced exudation. the larvae do not normally trench plant species such as plantain, plantago lanceolata, that lack exudate. to determine what cues elicit trenching, i reared larvae to the final instar on plantain, then applied test solutions to their mouthparts during feeding. loopers that received latex from lactuca serriola (asteraceae) or phloem exu ...	1997	28307485
substrate specificity modification of the stromal glycerol-3-phosphate acyltransferase.	the stromal glycerol-3-phosphate acyltransferases (gpats; ec 2.3.1.15) from spinach (spinacia oleracea) and squash (cucurbita moschata) were expressed in escherichia coli and their activities with palmitoyl-coa and oleoyl-coa compared. the gpat from squash, a chilling-sensitive plant, was found to have the greatest difference in activities between the two substrates, using palmitoyl-coa over three times faster than oleoyl-coa. in contrast, the enzyme from spinach, a chilling-tolerant plant, pref ...	1997	9016814
mutagenesis of squash (cucurbita moschata) glycerol-3-phosphate acyltransferase (gpat) to produce an enzyme with altered substrate selectivity.	in an attempt to rationalize the relationship between structure and substrate selectivity of glycerol-3-phosphate acyltransferase (gpat, 1at, ec 2.3.1.15) we have cloned a number of cdnas into the pet overexpression system using a pcr-based approach. following assay of the recombinant enzyme we noted that the substrate selectivity of the squash (cucurbita moschata) enzyme had altered dramatically. this form of gpat has now been crystallized and its full three-dimensional structure elucidated. si ...	2000	11171168
a second gene for acyl-(acyl-carrier-protein): glycerol-3-phosphate acyltransferase in squash, cucurbita moschata cv. shirogikuza(*), codes for an oleate-selective isozyme: molecular cloning and protein purification studies.	a new isogene for acyl-(acyl-carrier-protein):glycerol-3-phosphate acyltransferase (gpat; ec 2.3.1.15) in squash has been cloned and the gene product was identified as oleate-selective gpat. using pcr primers that could hybridise with exons for a previously cloned squash gpat, we obtained two pcr products of different size: one coded for a previously cloned squash gpat corresponding to non-selective isoforms at2 and at3, and the other for a new isozyme, probably the oleate-selective isoform at1. ...	2000	11134424
crystallization and preliminary crystallographic study of cucurmosin, a ribosome-inactivating protein from the sarcocarp of cucurbita moschata.	cucurmosin, a ribosome-inactivating protein purified from pumpkin, the sarcocarp of cucurbita moschata, has been crystallized using polyethylene glycol as a precipitant. the crystals belong to space group p2(1)2(1)2(1) and have unit-cell parameters a = 41.91, b = 59. 48, c = 98.78 a. there is one molecule in the asymmetric unit. the diffraction data to 3.0 a resolution were collected on a mar research image-plate detector.	2000	10771444
[immunocytological localization of iaa in the parenchyma cell and vascular elements in the graft union of cucurbita pepo/cucurbita moschata at the early developmental stage].	immuno-gold localization of iaa in cells of the graft union in the explant internode graft of cucurbita pepo/cucurbita moschata were investigated with electron microscopy. in parenchyma cells near the graft union, the gold particles were mainly accumulated in nucleus, plastid and endoplasmic reticulum, while no gold particles was detected in golgi body, mitochondrion, cell wall and vacuoles. in the differentiating xylem element, the gold particles were labeled in secondary wall and cytoplasm. in ...	2000	12548981
carotenoid composition and vitamin a value of an argentinian squash (cucurbita moschata).	the carotenoid composition of butternut squash (cucurbita moschata) cultivated in the province of santiago del estero, argentina, was determined. the main carotenoids isolated were identified as beta-carotene (beta,beta-carotene), alpha-carotene (beta,epsilon-carotene), and lutein (beta,epsilon-carotene-3,3'-diol) and the minor carotenoids, as phytofluene (7,8,11,12,7',8'-hexahydro-psi,psi-carotene), zeta-carotene (7,8,7',8'-tetrahydro-psi,psi-carotene), neurosporene (7,8-dihydro-psi,psi-caroten ...	2001	12012567
crystallization and preliminary x-ray analysis of the glycerol-3-phosphate 1-acyltransferase from squash (cucurbita moschata).	glycerol-3-phosphate 1-acyltransferase (e.c. 2.3.1.15; g3pat) catalyses the incorporation of an acyl group from either acyl-acyl carrier proteins (acylacps) or acylcoas into the sn-1 position of glycerol 3-phosphate to yield 1-acylglycerol 3-phosphate. crystals of squash g3pat have been obtained by the hanging-drop method of vapour diffusion using peg 4000 as the precipitant. these crystals are most likely to belong to space group p2(1)2(1)2(1), with approximate unit-cell parameters a = 61.1, b ...	2001	11223529
induction of cutinolytic esterase activity during saprophytic growth of cucurbit pathogens, fusarium solani f. sp. cucurbitae races one and two (nectria haematococca mpi and mpv, respectively).	cutins from fruit of cucurbita maxima and cucurbita moschata cultivars, apple and a c(16) alcohol (hexadecanol) were used to induce cutinolytic esterase activity during saprophytic growth of strains of the two cucurbit pathogens, fusarium solani f. sp. cucurbitae, race 1 (nectria haematococca mating population (mpi) and f. solani f. sp. cucurbitae, race 2 (mpv). four strains of mpv and 11 strains of mpi were were included in the study. although we were primarily interested in the two cucurbit pa ...	2001	11164297
purification and characterization of moschins, arginine-glutamate-rich proteins with translation-inhibiting activity from brown pumpkin (cucurbita moschata) seeds.	from fresh brown pumpkin seeds, two proteins with a molecular mass of 12kda and an n-terminal sequence rich in arginine and glutamate residues were obtained. the protein designated alpha-moschin closely resembled the fruitfly programmed-cell death gene product and the protein designated beta-moschin demonstrated striking similarity to prepro 2s albumin in n-terminal sequence. alpha- and beta-moschins inhibited translation in the rabbit reticulocyte lysate system with an ic(50) of 17 microm and 3 ...	2002	12356464
phylogenetic relationships among domesticated and wild species of cucurbita (cucurbitaceae) inferred from a mitochondrial gene: implications for crop plant evolution and areas of origin.	we have investigated the phylogenetic relationships among six wild and six domesticated taxa of cucurbita using as a marker an intron region from the mitochondrial nad1 gene. our study represents one of the first successful uses of a mtdna gene in resolving inter- and intraspecific taxonomic relationships in angiosperms and yields several important insights into the origins of domesticated cucurbita. first, our data suggest at least six independent domestication events from distinct wild ancesto ...	2002	11782554
kinetic mechanism and order of substrate binding for sn-glycerol-3-phosphate acyltransferase from squash (cucurbita moschata).	sn-glycerol-3-phosphate acyltransferase (g3pat, ec 2.3.1.15), a component of glycerolipid biosynthesis, is an important enzyme in chilling sensitivity in plants. the three-dimensional structure of the enzyme from squash (cucurbita moschata), without bound substrate, has been determined [turnbull et al. (2001) acta crystallogr. d 57, 451-453; turnbull et al. (2001) structure 9, 347-353]. here we report the kinetic mechanism of plastidial g3pat from squash and the order of substrate binding using ...	2002	11943166
germanium does not substitute for boron in cross-linking of rhamnogalacturonan ii in pumpkin cell walls.	boron (b)-deficient pumpkin (cucurbita moschata duchesne) plants exhibit reduced growth, and their tissues are brittle. the leaf cell walls of these plants contain less than one-half the amount of borate cross-linked rhamnogalacturonan ii (rg-ii) dimer than normal plants. supplying germanium (ge), which has been reported to substitute for b, to b-deficient plants does not restore growth or reduce tissue brittleness. nevertheless, the leaf cell walls of the ge-treated plants accumulated considera ...	2002	12481079
13-hydroxy-9z,11e,15e-octadecatrienoic acid from the leaves of cucurbita moschata.	a new unsaturated hydroxy fatty acid was isolated from the leaves of cucurbita moschata through repeated silica gel column chromatography and chemical methods. the structure of the new fatty acid was determined as 13-hydroxy-9,11,15-octadecatrienoic acid on the basis of several spectral data including 2d-nmr. the stererostructures of double bonds were determined to be 9z, 11e and 15e by coupling patterns of related proton signals in the 1h-nmr and noesy experiments.	2002	12214851
purification and characterization of moschatin, a novel type i ribosome-inactivating protein from the mature seeds of pumpkin (cucurbita moschata), and preparation of its immunotoxin against human melanoma cells.	a novel ribosome-inactivating protein designated moschatin from the mature seeds of pumpkin (cucurbita moschata) has been successively purified to homogeneity, using ammonium sulfate precipitation, cm-cellulose 52 column chromatography, blue sepharose cl-6b affinity column chromatography and fplc size-exclusion column chromatography. moschatin is a type 1 rip with a pi of 9.4 and molecular weight of approximately 29 kd. it is a rrna n-glycosidase and potently blocked the protein synthesis in the ...	2003	14672560
increase in zucchini yellow mosaic virus symptom severity in tolerant zucchini cultivars is related to a point mutation in p3 protein and is associated with a loss of relative fitness on susceptible plants.	abstract zucchini yellow mosaic virus (zymv, potyvirus) is a very damaging cucurbit virus worldwide. interspecific crosses with resistant cucurbita moschata have led to the release of "resistant" zucchini squash (c. pepo) f(1) hybrids. however, although the resistance is almost complete in c. moschata, the commercial c. pepo hybrids are only tolerant. zymv evolution toward increased aggressiveness on tolerant hybrids was observed in the field and was obtained experimentally. sequence comparisons ...	2003	18943611
characterization and activity enhancement of the phloem-specific pumpkin pp2 gene promoter.	the promoter of the pumpkin (cucurbita moschata) pp2 gene (designated np) was isolated from the restriction enzyme-digested genomic dna pool by genome walking and its activity and phloem specificity were examined in transgenic tobacco plants by using gus as a reporter. deletion analysis of the promoter revealed that the 473-bp fragment (-465 to + 8 relative to the transcription start site; designated as npii) exhibited similar activity as the full-length np promoter and retained its phloem speci ...	2004	15672837
use of cucumis metuliferus as a rootstock for melon to manage meloidogyne incognita.	root-knot nematode-susceptible melons (cantaloupe) were grown in pots with varying levels of meloidogyne incognita and were compared to susceptible melons that were grafted onto cucumis metuliferus or cucurbita moschata rootstocks. in addition, the effect of using melons as transplants in nematode-infested soil was compared to direct seeding of melons in nematode-infested soil. there were no differences in shoot or root weight, or severity of root galling between transplanted and direct-seeded n ...	2005	19262873
reassessing coxcatlan cave and the early history of domesticated plants in mesoamerica.	reanalysis and direct accelerator mass spectrometry radiocarbon dating of the cucurbit assemblage from coxcatlan cave provide information on the timing and sequence of the initial appearance of three domesticated plants in the tehuacán valley (puebla, mexico) and allow reassessment of the overall temporal context of plant domestication in mexico. cucurbita pepo is the earliest documented domesticate in the cave, dating to 7,920 calibrated calendrical (cal) years b.p. the bottle gourd (lagenaria ...	2005	15908505
[relationships of aulacophora beetles feeding behavior with cucurbitacin types in host crops].	aulacophora fermoralis chinensis and aulacophora cattigarensis are the two related aulacophora beetles, but their host selectivity and feeding behavior are significantly different. a. fernzoralis chinensis usually feeds upon cucumis sativus and cucurbita moschata, but never upon luffa acutangula, momordica charantia and citrullas lanatus. its feeding behavior on hosts is to snip a circular trench on their leaves, and then, to feed the leaf tissues isolated by the trench. on the contrary, a. catt ...	2005	16252877
[allelopathic effects of cultured cucurbita moschata root exudates].	by using the techniques of tissue culture, bio-assay and laboratory analysis, this paper studied the effects of the allelopathic chemicals from pumpkin (cucurbita moschata) roots on the seed germination and seedling growth of pumpkin, wheat (triticum aestivum), and radish (raphanus sativus). the pumpkin root was cultured on a sterile b5 media, and the concentrations of macro- and microelements, organic supplements and hormones in the media were adjusted by using an orthogonal design. after cultu ...	2005	16011180
[effects of root exudates of squash grafted with cucumber shoot on seed germination].	cucumber (cucumis statirus l.) is commonly cultivated by grafting on squash (cucurbita moschata) in commercial production. the effects of root exudates of squash grafted with cucumber on seed germination rate of cucumber and squash were tested. in this study, the exudates of cucumber-grafted squash roots were continuously collected with xad-4 resin, and were further diluted with distilled water to make 5%, 10%, 20% and 40% concentrations. cucumber and squash seeds were used for bioassay. the see ...	2005	15840942
new phenolic glycosides from the seeds of cucurbita moschata.	five new phenolic glycosides, cucurbitosides a-e (1-5), were isolated from the seeds of cucurbita moschata. their structures were elucidated as 2-(4-hydroxy)phenylethanol 4-o-(5-o-benzoyl)-beta-d-apiofuranosyl(1-->2)-beta-d-glucopyranoside (1), 2-(4-hydroxyphenyl)ethanol 4-o-[5-o-(4-hydroxy)benzoyl]-beta-d-apiofuranosyl(1-->2)-beta-d-glucopyranoside (2), 4-hydroxybenzyl alcohol 4-o-(5-o-benzoyl)-beta-d-apiofuranosyl(1-->2)-beta-d-glucopyranoside (3), 4-hydroxybenzyl alcohol 4-o-[5-o-(4-hydroxy)b ...	2005	15684523
phenolic profiles of raw apricots, pumpkins, and their purees in the evaluation of apricot nectar and jam authenticity.	the possibility of proving the undeclared addition of pumpkin puree in apricot nectars and jams has been investigated by using the phenol compound fingerprint and sensory evaluation. the cheaper pumpkin admixtures in apricot nectars and jams could not be detected by the sensory evaluation, particularly if present in quantities of <15%. the lower admixtures of pumpkin puree in apricot nectars and jams could be detected by the presence of syringic acid, a phenolic compound characteristic of the in ...	2005	15941324
copper and lead levels in two popular leafy vegetables grown around morogoro municipality, tanzania.	a study was carried out to determine the levels of two heavy metals, lead (pb) and copper (cu), in two popular leafy vegetables grown around morogoro municipality in tanzania. vegetable samples of pumpkin leaves (cucurbita moschata) and chinese cabbage (brassica chinensis) were collected from three sites and analysed for their concentrations of the two metals using an atomic absorption spectrophotometer. the three sites, namely mazimbu, kihonda and towelo are located within a 10 km perimeter. th ...	2006	17058799
[effects of nacl stress on cation contents in seedlings of two pumpkin varieties].	effects of different concentration nacl stress on cation contents of pumpkin cultivars (cucurbita ficifolia and cucurbita moschata) usually used as rootstocks of cucumber were studied. the results showed that, in both cultivars treated with 150, 300 and 500 mmol/l nacl for 7 d, increasing salinity caused in increasing retardation of shoot and root growth, and reduction of shoot relative water content (table 1). with increasing nacl concentration, na(+) content increased while k(+) content decrea ...	2006	16477137
analysis of gamma-irradiated melon, pumpkin, and sunflower seeds by electron paramagnetic resonance spectroscopy and gas chromatography-mass spectrometry.	seeds of melon (citrullus lanatus var. sp.), pumpkin (cucurbita moschata), and sunflower (heliantus annus) were gamma-irradiated at 1, 3, 5, and 10 kgy and analyzed by electron paramagnetic resonance (epr) and gas chromatography-mass spectrometry (gc-ms) according to en1787:2000 and en1785:2003, respectively. distinguishable triplet signals due to the presence of induced cellulose radicals were found at 2.0010-2.0047 g in the epr spectra. the gamma-irradiated radiolytic markers of 2-dodecylcyclo ...	2006	16968077
ribosome-inactivating proteins in edible plants and purification and characterization of a new ribosome-inactivating protein from cucurbita moschata.	the basic protein fraction of tissue extracts from 40 edible plants inhibited cell-free protein synthesis and released adenine from herring sperm dna, thus having adenine glycosylase activity. this suggested the presence of ribosome-inactivating proteins (rips) in the plant extracts. this indication was further strengthened by the presence of the two activities after a partial chromatographic purification of three extracts, including that from lycopersicon esculentum (tomato), which had very low ...	2006	16564632
food processing methods influence the glycaemic indices of some commonly eaten west indian carbohydrate-rich foods.	glycaemic index (gi) values for fourteen commonly eaten carbohydrate-rich foods processed by various methods were determined using ten healthy subjects. the foods studied were round leaf yellow yam (dioscorea cayenensis), negro and lucea yams (dioscorea rotundata), white and sweet yams (dioscorea alata), sweet potato (solanum tuberosum), irish potato (ipomoea batatas), coco yam (xanthosoma spp.), dasheen (colocasia esculenta), pumpkin (cucurbita moschata), breadfruit (artocarpus altilis), green ...	2006	16925852
[potential allelopathic effects of piper nigrum, mangifera indica and clausena lansium].	with piper nigrum, mangifera indica and clausena lansium as the donators, this paper studied their potential allelopathic effects on the germination and growth of zea mays, glycine max, cucurbita moschata, arachis hypogaea, raphanus sativus, echinochloa crusgalli, digitaria sanguinalis and stylosanthes guianensis. the results showed that the aqueous extracts of these donators could inhibit the germination and growth of z. mays, g. max, c. moschata, e. crus-galli and d. sanguinalis at high concen ...	2006	17147171
chemical composition and antioxidant activity of an acidic polysaccharide extracted from cucurbita moschata duchesne ex poiret.	a simple and sensitive high-performance capillary electrophoresis (hpce) method was designed for quantitative analysis of the component monosaccharides of an acidic polysaccharide extracted from pumpkin. in this method, the polysaccharide was hydrolyzed into component monosaccharides with 2.0 m trifluoroacetic acid at 100 degrees c for 6 h and then labeled with 1-phenyl-3-methyl-5-pyrazolone, and subsequently the labeled monosaccharide derivatives were separated by hpce. as a result, glucose (21 ...	2007	17511465
flowering locus t protein may act as the long-distance florigenic signal in the cucurbits.	cucurbita moschata, a cucurbit species responsive to inductive short-day (sd) photoperiods, and zucchini yellow mosaic virus (zymv) were used to test whether long-distance movement of flowering locus t (ft) mrna or ft is required for floral induction. ectopic expression of ft by zymv was highly effective in mediating floral induction of long-day (ld)-treated plants. moreover, the infection zone of zymv was far removed from floral meristems, suggesting that ft transcripts do not function as the f ...	2007	17540715
a water-soluble extract from cucurbita moschata shows anti-obesity effects by controlling lipid metabolism in a high fat diet-induced obesity mouse model.	during the screening of a variety of plant sources for their anti-obesity activity, it was found that a water-soluble extract, named pg105, prepared from stem parts of cucurbita moschata, contains potent anti-obesity activities in a high fat diet-induced obesity mouse model. in this animal model, increases in body weight and fat storage were suppressed by 8-week oral administration of pg105 at 500 mg/kg, while the overall amount of food intake was not affected. furthermore, pg105 protected the d ...	2007	17548058
preceramic adoption of peanut, squash, and cotton in northern peru.	the early development of agriculture in the new world has been assumed to involve early farming in settlements in the andes, but the record has been sparse. peanut (arachis sp.), squash (cucurbita moschata), and cotton (gossypium barbadense) macrofossils were excavated from archaeological sites on the western slopes of the northern peruvian andes. direct radiocarbon dating indicated that these plants grew between 9240 and 5500 (14)c years before the present. these and other plants were recovered ...	2007	17600214
content and in-vitro accessibility of pro-vitamin a carotenoids from sri lankan cooked non-leafy vegetables and their estimated contribution to vitamin a requirement.	vitamin a deficiency is a public health problem in sri lanka, which affects especially pre-school children. carrots (daucus carota), pumpkins (cucurbita maxima), squashes (cucurbita moschata) and sweet potatoes (ipomoea batatas) of orange, yellow-fleshed varieties are good sources of provitamin a carotenoids, but have not been studied in sri lanka in terms of the food as eaten. the content of carotenoids in each preparation method and the in-vitro accessibility of beta-carotene and alpha-caroten ...	2007	17852483
[comparative analysis of rdna distribution in metaphase chromosomes of cucurbitaceae species].	fluorescence in situ hybridization (fish) and double fish experiments were carried out to ascertain the chromosomal distribution patterns of the 45s and 5s ribosomal dnas in the three species of cucurbitaceae. five pairs of 45s rdna loci and two pairs of 5s rdna signals were detected on chromosomes of cucurbita moschata duch. luffa cylindrical roem. contained five pairs of 45s rdna loci and one pair of 5s rdna loci. in benincasa hispida cogn., two pairs of 45s rdna sites and one pair of 5s rdna ...	2007	17548333
qualitative and quantitative differences in carotenoid composition among cucurbita moschata, cucurbita maxima, and cucurbita pepo.	squashes and pumpkins are important dietary sources of carotenoids worldwide. the carotenoid composition has been determined, but reported data have been highly variable, both qualitatively and quantitatively. in the present work, the carotenoid composition of squashes and pumpkins currently marketed in campinas, brazil, were determined by hplc-dad, complemented by hplc-ms for identification. cucurbita moschata 'menina brasileira' and c. moschata 'goianinha' had similar profiles, with beta-carot ...	2007	17444652
hplc-dad-ms(n) characterisation of carotenoids from apricots and pumpkins for the evaluation of fruit product authenticity.	carotenoids including carotenoid esters from six apricot (prunus armeniaca l.) cultivars and from eight cultivars from three pumpkin species (cucurbita maxima duch., cucurbita pepo l., and cucurbita moschata duch.) were extracted without saponification, separated on a c-30 reversed-phase column and characterised by high-performance liquid chromatography/atmospheric pressure chemical ionisation-mass spectrometry (lc-ms). the predominant free carotenoids were quantified by hplc with diode array de ...	2008	26049248
functional group diversity of bee pollinators increases crop yield.	niche complementarity is a commonly invoked mechanism underlying the positive relationship between biodiversity and ecosystem functioning, but little empirical evidence exists for complementarity among pollinator species. this study related differences in three functional traits of pollinating bees (flower height preference, daily time of flower visitation and within-flower behaviour) to the seed set of the obligate cross-pollinated pumpkin cucurbita moschata duch. ex poir. across a land-use int ...	2008	18595841
atomic resolution structure of cucurmosin, a novel type 1 ribosome-inactivating protein from the sarcocarp of cucurbita moschata.	a novel type 1 ribosome-inactivating protein (rip) designated cucurmosin was isolated from the sarcocarp of cucurbita moschata (pumpkin). besides rrna n-glycosidase activity, cucurmosin exhibits strong cytotoxicities to three cancer cell lines of both human and murine origins, but low toxicity to normal cells. plant genomic dna extracted from the tender leaves was amplified by pcr between primers based on the n-terminal sequence and x-ray sequence of the c-terminal. the complete mature protein s ...	2008	18652900
starch grains on human teeth reveal early broad crop diet in northern peru.	previous research indicates that the nanchoc valley in northern peru was an important locus of early and middle holocene human settlement, and that between 9200 and 5500 (14)c yr b.p. the valley inhabitants adopted major crop plants such as squash (cucurbita moschata), peanuts (arachis sp.), and cotton (gossypium barbadense). we report here an examination of starch grains preserved in the calculus of human teeth from these sites that provides direct evidence for the early consumption of cultivat ...	2008	19066222
tritrophic effects on survival and development in a coccidophagous coccinellid.	chilocorus nigritus is currently considered one of the most successful biological control agents of armoured scale insects. however, establishment of this beetle in crop pest situations has not always been successful and there are still gaps in our knowledge of its ecology and behaviour. the research involved an examination of tritrophic effects on the survival and development of this common diaspid predator. the effect of a forced change in host plant on the developmental time of the juvenile s ...	2008	19226777
ssr-based genetic linkage map of cucurbita moschata and its synteny with cucurbita pepo.	the first ssr-based genetic linkage map of cucurbita moschata was created by integrating the maps of two f2 populations with one common parent developed from the crosses waltham butternut (wb) x nigerian local (nl) and zhou (a hull-less type) x wb. the integrated c. moschata map comprises 205 ssr markers and two morphological traits (gr and n). the map is composed of 27 linkage groups with a marker density of 7 cm. comparing the c. moschata map with the published cucurbita pepo map, we found a h ...	2008	18956020
[effects of nacl stress on cation contents in different pumpkin cultivars' seedlings].	with the seedlings of 19 pumpkin cultivars as test materials, this paper studied the variations of na+, k+, ca2+, na+/k+, na+/ca2+, sn+, k+ and sna+, ca2+ in their shoots and roots under the stress of 300 mmol nacl x l(-1). the results showed that after an 8-day exposure to 300 mmol nacl x l(-1), the na+ content in the seedlings increased significantly while the k+ content decreased, resulting in the brokenness of ion balance. the root na+ content, shoot na+/k+ and na+/ca2+ ratios, and sna+, k+ ...	2008	18533527
[effects of nacl stress on free polyamines content and reactive oxygen species level in pumpkin roots].	taking cucurbita moschata duch hybrid 360-3 x 112-2 and c. ficifolia bouche as test materials, the effects of nacl stress on their plant growth and the o2*- production rate and h2o2 and free polyamines (pas) contents in their roots were studied with hydroponic culture. the results showed that after 10 d nacl stress, the plant growth of the two pumpkin varieties was strongly inhibited, compared with the control, and c. ficifolia was more injured than hybrid 360-3 x 112-2. under nacl stress, the r ...	2008	19102314
location of caspase 3-like protease in the development of sieve element and tracheary element of stem in cucurbita moschata.	the casepase is considered to regulate the process of programmed cell death in the development of organisms. in this study, caspase 3-like protease was detected by immunohistochemistry and immunoelectron microscopy during the development of sieve element and tracheary element of stem in cucurbita moschata duch. antibody with brown color (under light microscopy) and gold particles (under transmission electron microscopy) for detecting caspase 3-like protease was mainly displayed in inner phloem, ...	2008	19093968
excellent combination of counter-current chromatography and preparative high-performance liquid chromatography to separate galactolipids from pumpkin.	galactolipids in the fruits of cucurbita moschata (pumpkin) could not be completely separated by high-performance liquid chromatography (hplc). preparative hplc was not available for preparing major galactolipid monomers in pumpkin. in the present paper, a combination of high-speed counter-current chromatography (hsccc) and preparative hplc was used for preparing the galactolipids. a fraction containing galactolipids (fr60) from the purification of the n-butanol extract of pumpkin by macro-porou ...	2009	19081570
new phenolic glycosides from the seeds of cucurbita moschata.	two new phenolic glycosides were isolated from the seeds of cucurbita moschata. their structures were elucidated as (2-hydroxy)phenylcarbinyl 5-o-benzoyl-beta-d-apiofuranosyl(1-->2)-beta-d-glucopyranoside (1) and 4-beta-d-(glucopyranosyl hydroxymethyl)phenyl 5-o-benzoyl-beta-d-apiofuranosyl(1-->2)-beta-d-glucopyranoside (2) on the basis of spectroscopic analysis and chemical evidence.	2009	20183300
structures of new phenolic glycosides from the seeds of cucurbita moschata.	a new phenolic glycoside and three known compounds were isolated from the seeds of cucurbita moschata. the structures of the new compound was elucidated as phenylcarbinyl 5-o-(4-hydroxy)benzoyl-beta-d-apiofuranosyl (1-->2)-beta-d-glucopyranoside on the basis of spectroscopic analysis and chemical evidence. three known compounds were identified as 1-o-benzyl[5-o-benzoyl-beta-d-apiofuranosyl(1-->2)]-beta-d-glucopyranoside 2, cucurbitosides c 3 and a 4, by comparison of the spectral data with repor ...	2009	19475995
morphological, biochemical and molecular characterization of herpetomonas samuelpessoai camargoi n. subsp., a trypanosomatid isolated from the flower of the squash cucurbita moschata.	we report the morphological, biochemical and molecular characteristics of a trypanosomatid isolated from the flower of cucurbita moschata. although the trypanosomatid was isolated from a plant, the lack of recognition of phytomonas-specific molecular markers based on spliced-leader and ribosomal genes as well as by monoclonal antibodies specific for phytomonas argues against assigning it to this genus. because the isolate displayed typical opisthomastigote forms in culture, it is assigned to the ...	2009	11249194
a molecular model for diacylglycerol acyltransferase from mortierella ramanniana var. angulispora.	acyl coa diacylglycerol acyltransferase (dgat, ec 2.3.120) is recognized as a key player of cellular diacylglycerol metabolism. it catalyzes the terminal, yet the committed step in triacylglycerol synthesis using diacylglycerol and fatty acyl coa as substrates. the protein sequence of diacylglycerol acyltransferse (dgat) type 2b in moretierella ramanniana var. angulispora (protein_id = aak84180.1) was retrieved from genbank. however, a structure is not yet available for this sequence. the 3d str ...	2009	19759814
lectins but not antifungal proteins exhibit anti-nematode activity.	a variety of lectins and antifungal proteins were tested for toxicity against the plant parasitic nematodes ditylenchus dipsaci and heterodera glycines. it was found that lectins from the mushrooms xylaria hypoxylon, agrocybe cylindracea and tricholoma mongolicum (tml-1) were the most potent against d. dipsaci, with ec(50) being 4.7, 9, and 20mg/ml, respectively. lectins from pseudostellaria heterophylla, samta tomato, and the mushrooms t. mongolicum (tml-2), ganoderma lucidum, and boletus eduli ...	2009	21784014
in vitro effects of cucurbita moschata seed extracts on haemonchus contortus.	this study was carried out to evaluate the in vitro effect of cucurbita moschata seed against the parasitic nematode of small ruminants haemonchus contortus. three extracts (aqueous, methanolic and dichloromethane) of c. moschata seed were tested in vitro on four developmental stages of h. contortus using egg hatch assay (eha), larval development assay (lda), l3 migration inhibition (lmi) assay and adult worm motility (awm) assay. the highly significant (p<0.001) ability to stop larval developme ...	2009	19135803
evaluation of alternatives to methyl bromide in melon crops in guatemala.	the monoculture of melon in guatemala has caused the massive appearance of plants with an analogous syndrome for the well-known disease commonly called melon collapse, or vine decline, causing significant losses in crops. methyl bromide is commonly used to sterilize soil prior to planting in guatemala, but it must be phased out by 2015. the objective of this study was to evaluate the technique of grafting melon onto hybrids of cucurbita (cucurbita maxima x cucurbita moschata), as an alternative ...	2009	18998189
effect of reproductive mode on host plant utilization of melon aphid (hemiptera: aphididae).	variation in the reproductive mode of melon aphid aphis gossypii glover occurred on the large geographic scale, but the performance of different reproductive modes to use host plant is poorly understood. life tables of melon aphid population that undergo the anholocyclic, androcyclic, and intermediate reproductive mode were conducted on different host plants. the results showed that the anholocyclic and androcyclic strains could become adults and produce offspring on cotton gossypium hirsutum l. ...	2010	22182553
[entomophilic pollination of squash, cucurbita moschata (cucurbitaceae)].	the objectives of this work were to determine the squash entomofauna in the region of viçosa, minas gerais state, to study their behavior on flowers and their importance for pollination, verifying the role of each pollinator. the most common species were trigona spinipes (fabricius), trigona hyalinata (lepeletier), apis mellifera (l.) and melipona quadrifasciata (lepeletier). the visitation behavior of a. mellifera, m. quadrifasciata, and bombus morio (swederus) were similar. they visited flower ...	2010	20498949
characterization of acid-extracted pectin-enriched products obtained from red beet (beta vulgaris l. var. conditiva) and butternut (cucurbita moschata duch ex poiret).	chemical and rheological characteristics of fractions enriched in soluble dietary fiber are reported. these fractions were obtained through acid hydrolysis of butternut (cucurbita moschata duch ex poiret) and red beet (beta vulgaris l. var. conditiva) cell wall enriched powders. hydrolysis was performed using citric acid at different ph values and reaction times (2 and 3 h). yields obtained for butternut fractions were between 21 and 28 g/100 g; for red beet, yields were 24 and 31 g/100 g for ph ...	2010	20178319
molecular cloning and expression of a bush related cmv1 gene in tropical pumpkin.	a bush-type plant was selected from tropical pumpkin 'cga' (cucurbita moschata duchesne) in order to study the vine development in c. moschata. in this study, a novel gene encoding nadh dehydrogenase was isolated from the vine line (cgav) of c. moschata, that was not expressed in the near isogenic bush line (cgabu). this gene, designated as cmv1 (c. moschata vine 1), was 545 bp in length and was composed of a 477 bp open reading frame, which had 99% nucleotide similarity to the chloroplast ndhj ...	2010	19308673
glucose-lowering activity of novel tetrasaccharide glyceroglycolipids from the fruits of cucurbita moschata.	two new tetrasaccharide glyceroglycolipids were obtained from pumpkin. the structures of the two compounds were determined using chemical methods and spectroscopic analysis. both compounds demonstrated significant glucose-lowering effects in streptozotocin- and high-fat-diet-induced diabetic mice.	2010	21215628
chlorotic curly stunt: a severe begomovirus disease of bottle gourd in northern india.	bottle gourd (lagenaria siceraria) an important vegetable crop in india was observed to be affected by a chlorotic curly stunt disease (ccsd) during 2003-2006 in the vegetable growing areas of delhi and adjoining state of haryana. the affected plants are severely stunted and bear very small chlorotic and mildly curled leaves. incidence of the disease varied from 4.7 to 36%. the disease could be easily transmitted by whitefly, bemisia tabaci but not by sap. the causal virus was found to be a bego ...	2010	23637479
nutritional, sensory and physical analysis of pumpkin flour incorporated into weaning mix.	the objective of this study was to develop a cereal-pulse complementary food fortified with different concentrations of pumpkin powder (cucurbita moschata), and to analyse its sensory and physic-chemical parameters. fresh pumpkins(cucurbita moschata) were procured from the market and dehydrated and powdered in the laboratory. sorghum (sorghum vulgare) and whole green gram (vigna radiate) were germinated, dried, pulverised and combined with powdered rice (oryza sativa) in the ratio of 2:1:1. pump ...	2010	22691991
olfactory cues from plants infected by powdery mildew guide foraging by a mycophagous ladybird beetle.	powdery mildews (erysiphales) are economically important plant pathogens that attack many agricultural crops. conventional management strategies involving fungicide application face challenges, including the evolution of resistance and concerns over impacts on non-target organisms, that call for investigation of more sustainable alternatives. mycophagous ladybird beetles (coleoptera: coccinellidae) feed on powdery mildew and have considerable potential as biological control agents; however, the ...	2011	21876772
silicon efflux transporters isolated from two pumpkin cultivars contrasting in si uptake.	the accumulation of silicon (si) differs greatly with plant species and cultivars due to different ability of the roots to take up si. in si accumulating plants such as rice, barley and maize, si uptake is mediated by the influx (lsi1) and efflux (lsi2) transporters. here we report isolation and functional analysis of two si efflux transporters (cmlsi2-1 and cmlsi2-2) from two pumpkin (cucurbita moschata duch.) cultivars contrasting in si uptake. these cultivars are used for rootstocks of bloom ...	2011	21617377
microplot evaluation of rootstocks for control of meloidogyne incognita on grafted tomato, muskmelon, and watermelon.	microplot experiments were conducted over two years (four growing seasons) to evaluate meloidogyne incognita resistance in rootstocks used for grafted tomato (solanum lycopersicum), muskmelon (cucumis melo), and watermelon (citrullus lanatus). three tomato rootstocks; 'tx301', 'multifort', and 'aloha', were tested in addition to the nongrafted scion, 'florida-47'. two muskmelon rootstocks; cucumis metuliferus and 'tetsukabuto' (cucurbita maxima × cucurbita moschata) were evaluated with the nongr ...	2011	23431109
agrobacterium-mediated transformation of kabocha squash (cucurbita moschata duch) induced by wounding with aluminum borate whiskers.	an efficient genetic transformation method for kabocha squash (cucurbita moschata duch cv. heiankogiku) was established by wounding cotyledonary node explants with aluminum borate whiskers prior to inoculation with agrobacterium. adventitious shoots were induced from only the proximal regions of the cotyledonary nodes and were most efficiently induced on murashige-skoog agar medium with 1 mg/l benzyladenine. vortexing with 1% (w/v) aluminum borate whiskers significantly increased agrobacterium i ...	2011	21400224
genes for resistance to zucchini yellow mosaic in tropical pumpkin.	four cultigens of cucurbita moschata resistant to zucchini yellow mosaic virus were crossed with the susceptible 'waltham butternut' and with each other in order to clarify the mode of inheritance of resistance and relationships among the genes involved. five loci were segregating, with genes for resistance zym-0 and zym-4 carried by 'nigerian local' and one of them also carried by 'nicklow's delight,' zym-1 carried by 'menina,' and zym-6 carried by 'soler.' a recessive gene carried by 'waltham ...	2011	21493595
potential mechanisms of hypocholesterolaemic effect of thai spices/dietary extracts.	several thai spices/dietary ingredients were previously shown to have hypocholesterolaemic effects. these studies were mostly conducted in animal models in which the mechanisms of action were not yet well-established. therefore, this study aimed to investigate the potential mechanism of hypocholesterolaemic action of 12 selected plants, namely hibiscus sabdariffa l., moringa oleifera lam., cucurbita moschata duchesne, ananas comosus (l.) merr., zingiber officinale, morus alba l., camellia sinens ...	2011	20623425
cucurmosin induces apoptosis of bxpc-3 human pancreatic cancer cells via inactivation of the egfr signaling pathway.	pancreatic cancer remains the fourth most common cause of cancer-related death in the united states. potent therapeutic strategies are urgently needed for pancreatic cancer. cucurmosin is a novel type 1 ribosome-inactivating protein (rip) isolated from the sarcocarp of cucurbita moschata (pumpkin). due to its cytotoxicity, cucurmosin can inhibit tumor cell proliferation through induction of apoptosis on tumor cells, but the specific mec ...	2011	22139427
tomato leaf curl new delhi virus is associated with pumpkin leaf curl: a new disease in northern india.	during 2006, pumpkin leaf curl-a new disease was observed in the experimental field at indian agricultural research institute. the disease was characterized by upward leaf curl with chlorotic patches and stunting of plant. polymerase chain reaction (pcr) with coat protein specific primers to tomato leaf curl new delhi virus (tolcndv) indicated association of a begomovirus with the disease. the sequence comparison and phylogenetic analysis of the complete dna genome further revealed the identity ...	2011	23730002
molecular characterization of tomato leaf curl palampur virus and pepper leaf curl betasatellite naturally infecting pumpkin (cucurbita moschata) in india.	pumpkin cultivation in india is affected by severe incidence of a yellow vein mosaic disease. tomato leaf curl new delhi virus and squash leaf curl china virus are known to be associated with this disease in india. we were able to identify a third begomovirus-tomato leaf curl palampur virus (tolcpmv), from pumpkin showing typical symptoms of the disease at varanasi based on the sequence of complete dna-a genome of the virus. the complete dna-a sequence of the virus shared more than 99% sequence ...	2011	23637491
changes in carotenoids during processing and storage of pumpkin puree.	changes in the contents of carotenoids and their true retentions (% tr) during the production of puree of cucurbita moschata 'menina brasileira' and of cucurbita maxima 'exposição' pumpkins and the stability of such compounds during 180days of storage were monitored by liquid chromatography coupled with a photodiode array detector. cooking caused higher losses than commercial sterilisation. high losses of xanthophylls such as lutein and violaxanthin were noted during processing and storage of pu ...	2011	25214348
isolation and functional characterization of an influx silicon transporter in two pumpkin cultivars contrasting in silicon accumulation.	a high accumulation of silicon (si) is required for overcoming abiotic and biotic stresses, but the molecular mechanisms of si uptake, especially in dicotyledonous species, is poorly understood. herein, we report the identification of an influx transporter of si in two cucurbita moschata (pumpkin) cultivars greatly differing in si accumulation, which are used for the rootstocks of bloom and bloomless cucumis sativus (cucumber), respectively. heterogeneous expression in both xenopus oocytes and r ...	2011	21205032
carotenoid accumulation and carotenogenic gene expression during fruit development in novel interspecific inbred squash lines and their parents.	carotenoid levels and composition during squash fruit development were compared in cucurbita moschata , cucurbita maxima , and two lines of their interspecific inbred lines, namely, maxchata1 and maxchata2. eight genes associated with carotenoid biosynthesis were analyzed by quantitative rt-pcr. the two squash species and their interspecific inbred lines exhibited different qualitative and quantitative carotenoid profiles and regulatory mechanisms. c. moschata had the lowest total carotenoid con ...	2012	22574777
characterization of the small rna component of leaves and fruits from four different cucurbit species.	micrornas (mirnas) are a class of non-coding small rnas involved in post-transcriptional regulation of gene expression critical for plant growth and development, stress responses and other diverse biological processes in plants. the cucurbitaceae or cucurbit family represents some of economically important species, particularly those with edible and medicinal fruits. genomic tools for the molecular analysis of members of this family are just emerging. partial draft genome sequence became availab ...	2012	22823569
dehydrodiconiferyl alcohol isolated from cucurbita moschata shows anti-adipogenic and anti-lipogenic effects in 3t3-l1 cells and primary mouse embryonic fibroblasts.	a water-soluble extract from the stems of cucurbita moschata, code named pg105, was previously found to contain strong anti-obesity activities in a high fat diet-induced obesity mouse model. one of its biological characteristics is that it inhibits 3t3-l1 adipocytes differentiation. to isolate the biologically active compound(s), conventional solvent fractionation was performed, and the various fractions were tested for anti-adipogenic activity using oil red o staining method. a single spot on t ...	2012	22262865
complete nucleotide sequence of watermelon chlorotic stunt virus originating from oman.	watermelon chlorotic stunt virus (wmcsv) is a bipartite begomovirus (genus begomovirus, family geminiviridae) that causes economic losses to cucurbits, particularly watermelon, across the middle east and north africa. recently squash (cucurbita moschata) grown in an experimental field in oman was found to display symptoms such as leaf curling, yellowing and stunting, typical of a begomovirus infection. sequence analysis of the virus isolated from squash showed 97.6-99.9% nucleotide sequence iden ...	2012	22852046
pumpkin (cucurbita moschata) fruit extract improves physical fatigue and exercise performance in mice.	pumpkin (cucurbita moschata) is a popular and nutritious vegetable consumed worldwide. the overall purpose of this study was to evaluate the effects of c. moschata fruit extract (cme) on anti-fatigue and ergogenic functions following physiological challenges. male icr mice from four groups designated vehicle, cme-50, cme-100 and cme-250, respectively (n = 8 per group in each test) were orally administered cme for 14 days at 0, 50, 100 and 250 mg/kg/day. the anti-fatigue activity and exercise per ...	2012	23047485
bioactive compounds in lipid fractions of pumpkin (cucurbita sp) seeds for use in food.	seeds are considered to be agro-industrial residues, which can be used as source of macronutrients and/or raw material for extraction of vegetable oils, since they present great quantities of bioactive compounds. this study aimed to characterize the lipid fractions and the seeds of pumpkin (cucurbita sp) varieties nova caravela, mini paulista, menina brasileira, and moranga de mesa aiming at using them in food. the chemical composition of the seeds was performed according to the official methods ...	2012	22671521
distinct genetic lineages of bactrocera caudata (insecta: tephritidae) revealed by coi and 16s dna sequences.	the fruit fly bactrocera caudata is a pest species of economic importance in asia. its larvae feed on the flowers of cucurbitaceae such as cucurbita moschata. to-date it is distinguished from related species based on morphological characters. specimens of b. caudata from peninsular malaysia and indonesia (bali and lombok) were analysed using the partial dna sequences of cytochrome c oxidase subunit i (coi) and 16s rrna genes. both gene sequences revealed that b. caudata from peninsular malaysia ...	2012	22615962
modeling and optimization of ultrasound-assisted extraction of polysaccharide from cucurbita moschata.	polysaccharides from pumpkin were extracted by ultrasound-assisted extraction technology using four factors at five levels central composite rotatable response surface design (ccrd). on using single factor analysis, process variables such as extraction temperature (50-70 °c), power of ultrasound (50-70 w), time (15-25 min) and solid-liquid ratio (1:10-1:20 g/ml) were selected. experiments were conducted to evaluate the effects of four independent variables on the maximum extraction yield of poly ...	2013	23399253
phytochemicals and antioxidative enzymes defence mechanism on occurrence of yellow vein mosaic disease of pumpkin (cucurbita moschata).	pumpkin (cucurbita moschata) samples showing yellow vein mosaic disease in varanasi region were identified with begomovirus infection using pcr amplification. a sequencing analysis of the full genome revealed that it is a strain of tomato leaf curl palampur virus (genbank id. fj931537). phytochemical composition and antioxidative enzyme levels were compared in infected and healthy plants. the study revealed that the amount of total protein declined in the infected leaves but elevated up to 135 % ...	2013	28324587
phloem long-distance delivery of flowering locus t (ft) to the apex.	cucurbita moschata flowering locus t-like 2 (hereafter ftl2) and arabidopsis thaliana (arabidopsis) flowering locus t (ft), components of the plant florigenic signaling system, move long-distance through the phloem from source leaves to the vegetative apex where they mediate floral induction. the mechanisms involved in long-distance trafficking of ft/ftl2 remain to be elucidated. in this study, we identified the critical motifs on both ft and ftl2 required for cell-to-cell trafficking through mu ...	2013	23607279
[effects of host plants on the life table parameters of experimental populations of aphis gossypii].	a comparative study was conducted on the life table parameters of aphis gossypii reared on five host plant species at (25 +/- 1) degrees c in laboratory. there existed significant differences in the durations of various developmental stages, adult longevity, mean offspring number per day, net reproductive rate, intrinsic rate of increase, finite rate of increase, mean generation time, and population doubling time for the a. gossypii populations reared on the host plants. for the aphids on lagena ...	2013	24015567