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postharvest gaseous ozone treatment enhances quality parameters and delays softening in cantaloupe melon during storage at 6 °c.this trial shows postharvest gaseous ozone treatment to evaluate the quality parameters and cell wall enzymes during the cantaloupe melon cv caldeo storage at 6 °c for 13 days.028612399
mapping quantitative trait loci for fruit traits and powdery mildew resistance in melon (cucumis melo).fruit characters affect consumer preferences and the market value of melons is determined by fruit quality. most fruit quality-related traits are controlled by multiple genes, and are influenced by environmental factors. furthermore, powdery mildew is another limiting factor in melon production. to develop new melon cultivars with disease resistance and high quality fruits using the molecular marker-assisted breeding strategy, identification of quantitative trait loci for fruit quality and disea ...028597428
quantitative trait loci analysis of melon (cucumis melo l.) domestication-related traits.loci on lgiv, vi, and viii of melon genome are involved in the control of fruit domestication-related traits and they are candidate to have played a role in the domestication of the crop. the fruit of wild melons is very small (20-50 g) without edible pulp, contrasting with the large size and high pulp content of cultivated melon fruits. an analysis of quantitative trait loci (qtl) controlling fruit morphology domestication-related traits was carried out using an in vitro maintained f2 populatio ...028584902
the influence of alcohol upon the growth of seedlings.in this paper it is shown that if the dry seeds of the cantaloupe (cucumis melo) are soaked for 3 hours in solutions of ethyl alcohol of concentration ranging from 2 to 16 per cent by volume, and then germinated and grown in distilled water in the dark, the total growth attained is greater by amounts ranging from 9 to 35 per cent than is that made by seeds treated in every way identically except that they are initially soaked in distilled water instead of alcohol. it is shown that this result is ...192619872195
the form of the growth curve of the canteloup (cucumis melo) under field conditions. 192816587417
the growth of seedlings of the canteloup, cucumis melo, in the absence of exogenous food and light. 192816587293
available food, relative growth and duration of life in seedlings of cucumis melo. 193316587762
the growth of cucumis melo seedlings at different temperatures. 193419872807
polysomaty in cucumis melo. 193916577910
refractive index as an estimate of quality between and within muskmelon fruits. 194116653723
a seed-borne virus of muskmelon. 194718895500
genetic and chlorophyll studies of a yellow-green mutant in muskmelon. 195216654451
sex expression and growth in muskmelon. 196016655417
longevity of fusarium oxysporum in soil tube culture.in soil tube culture, representatives of three biologic forms of fusarium oxysporum survived unchanged morphologically for 11 years or more. an isolate of the muskmelon wilt fungus remained viable after 17 years' storage in dry air at a temperature of from 3 degrees to 4 degrees c. the surviving unit was found to be the chlamydospore.196117800128
a soil suspension-plating method of estimating populations of fusarium oxysporum f. melonis in muskmelon wilt soils. 196214006148
[research on plant viruses. virus card xii: chlorotic spotting of the melon (cucumis melo l.)]. 196414221468
graft-induced sex changes in cucumis melo l. 196414156326
influence of carbohydrate and nitrogenous amendments to soil on incidence of muskmelon wilt and populations of the wilt fungus. 19655879509
some relationship between plant nutrition, fungal populations, and incidence of fusarium wilt of muskmelon. 196514346135
influence of resistant and susceptible varieties of muskmelon on size of populations of the fusarium wilt fungus and wilt in naturally infested soils. 19665963326
the effect of prewetting naturally infested soils on destruction of the muskmelon wilt fungus by formaldehyde. 19685689582
experiments in biological and chemical control of fusarium wilt (f. oxysporum f. melonis (leach and currence) snyd. and hans.) of muskmelon. 19695344745
increase in femaleness of three cucurbits by treatment with ethrel, an ethylene releasing compound.the application of ethrel (2-chloroethane phosphonic acid), an ethylene-releasing compound, to monoecious cultivars of cucumber and squash and an andromonoecious cultivar of muskmelon, caused a shift towards femaleness in all three species. the increase in femaleness manifested itself in several symptoms: a decrease in the number of staminate (male) flowers, an increase in the number of pistillate (female) or hermaphrodite (perfect) flowers, and a change in flowering pattern, namely, formation o ...196924515743
[a case of a muskmelon like corneal change due to the wearing of a contact lens]. 19695390928
control of fusarium wilt of muskmelon and other effects of benomyl soil drenches. 19705471339
pharmacodynamic investigations into the diuretic activity of cucumis melo seed (ether extract). 19705506675
isolation and identification of the gibberellins of cucumis sativus and cucumis melo.thin-layer chromatography, gas-liquid chromatography, and mass spectrometry were used to identify gibberellins isolated from mature seeds of both cucumis sativus (cucumber) and cucumis melo (muskmelon). the gibberellins were extracted and purified by organic solvent fractionation, paper and thin-layer chromatography, and crystallization. seeds of c. sativus were found to contain gibberellins a1, a3, a4, and a7 with a1 the predominant species. seeds of c. melo contained gibberellins a1 and a3 and ...197224481557
ethylene: a natural regulator of sex expression of cucumis melo l.sex expression in cucumber (cucumis sativus l.) and muskmelon (c. melo l.) was correlated with endogenous ethylene production. plants of gynoecious (all female) sex types of the two species produced more ethylene than monoecius (male-female) plants. c. melo plants of a gynoecious sex type that normally produce only pistillate (female) flowers, when grown with hypobaric ventilation to facilitate removal of endogenous gases by diffusion, produced perfect (hermaphroditic) flowers. when either the p ...197216591971
diurnal variations in photosynthetic products and nitrogen metabolism in expanding leaves.expanding leaves of capsicum frutescens l. cv. california wonder, cucumis melo l. cv. hales best, and citrus sinensis l. osbeck cv. washington navel showed a marked diurnal periodicity in the incorporation of (14)c from photosynthetically fixed (14)co(2) into amino acids. incorporation was virtually nil at the beginning of the photoperiod, reached a maximum in the 6th to 7th hour and decreased during the latter part of the photoperiod. in capsicum frutescens this was apparently a reflection of t ...197316658402
novel properties of satellite dna from muskmelon.the purified dense satellite of muskmelon (cucumis melo l.) represents 30% of the total dna and forms a sharp, unimodal peak in both neutral and alkaline cscl gradients. spectrophotometric melting and dna reassociation analyses revealed that one-third of the satellite is high melting and has a complexity of about 2.5 x 10(5) daltons, while the remaining two-thirds of the satellite melts 8 degrees lower and has a complexity of about 5 x 10(8) daltons. the thermal stability of reassociated satelli ...19744524654
isolation and characterization of a proteinase from the sarcocarp of melon fruit.a proteinase from the sarcocarp of melon (cucumis melo l. var. prince) was purified by a three-step procedure involving batch-wise treatment with cm-cellulose fibers, column chromatography on cm-cellulose powder and gel filtration on sephadex g-75. the final enzyme preparation was homogeneous on acrylamide gel electrophoresis. its molecular weight was estimated by two different methods to be about 50,000. anlayses indicated tha presence of 475 amino acid residues and at least 7 moles of hexose. ...19755423
the complexity of satellite deoxyribonucleic acid in a higher plant.purified satellite dna from melon (cucumis melo) was shown to contain at least two components from thermal-denaturation and renaturation studies. two components were separated after partial renaturation, a fast-renaturing fraction similar in complexity to mouse satellite dna, and one with 6000 times greater complexity. both components renatured very accurately, indicating a minimum of sequence divergence. centrifugation of the purified satellite dna in ag+/cs2so4 gradients resolved two major and ...19751191264
pathogenicity and histopathology of rotylenchulus reniformis infecting cantaloup.rotylenchulus reniformis was pathogenic to cantaloup (cucumis melo 'perlita') under greenhouse conditions. these findings confirm field symptoms of cantaloup infected with r. reniformis. histopathological studies show that the nematode penetrates the cortex perpendicular to the vascular system and comes to rest with the head against the endodermis in young roots. feeding stimulated the pericycle to either side of the endodermal feeding cell and caused cell hypertrophy with enlargement of the nuc ...197519308147
the heterogeneity of phloem exudate proteins from different plants: a comparative survey of ten plants using polyacrylamide gel electrophoresis.proteins in sieve tube exudate from ricinus communis l., acer pseudoplatanus l., aesculus hippocastanum l., cucumis melo l., and two cultivars each of cucumis sativus l., cucurbita pepo l. and cucurbita maxima duchesne were fractionated and compared using polyacrylamide gel electrophoresis. striking differences in major exudate proteins were displayed among the genera and species examined. even cultivars within a single species, although showing general similarities, differed in some prominent p ...197624424985
a comparative analysis of phloem exudate proteins from cucumis melo, cucumis sativus and cucurbita maxima by polyacrylamide gel electrophoresis and isoelectric focusing.proteins in sieve tube exudate from cucumis melo l., cucumis sativus l. and cucurbita maxima duch. were analysed by gel electrophoresis and isoelectric focusing. estimated molecular weights and isoelectric points for the major and minor proteins from each plant species are presented. electrophoresis revealed striking differences between the protein complements of exudatc from the two genera investigated. similarly, although a few exudate proteins from the two species of cucumis possessed identic ...197624424601
modifications induced by benomyl and related compounds into chloroplasts spectral patterns, photosynthetic rates and chlorophyll contents of spinacia oleracea and cucumis melo. 1977861396
sequence arrangement in satellite dna from the muskmelon.two fractions of a satellite dna from the muskmelon (cucumis melo l.) isolated as a unimodal peak from cscl gradients, differ in melting properties and complexity as estimated by reassociation kinetics. at 49.8 c, all of the low melting fraction was denatured and all of the high melting fraction was native. there were almost no partially denatured molecules detected in the electron microscope at this temperature. this observation provides direct evidence that the two fractions are not closely li ...197716659902
occurrence of patulin in fruit and vegetables.in different varieties of apples and pears suffering from brown rot, patulin was found in about 50 p. 100 of samples investigated (about 120). patulin levels as high as 1 g/kg rotten material were found 2-3 days after the fruit was removed from the cold store where it had been stored for 5 months. a significant diffusion into the healthy plant tissue was not observed in apples, but in peaches, tomatoes and pears. vegetable products with natural patulin content: apples, pears, peaches, apricots, ...1977613910
[patulin in foods of vegetable origin. 2. several kinds of fruit and vegetables and fruit and vegetable products (author's transl)].patulin was found in fruit with spontaneous brown rot (bananas, pineapples, grapes, peaches, apricots) as well as in moldy compots and in sallow-thorn juice. fruit, vegetables and fruit and vegetable product were artificially infected with penicillium expansum, p. urticae and byssochlamys nivea; patulin was subsequently found in peaches, apricots, greengages, bananas, strawberries, honeydew melons, tomatoes, red and green paprika, cucumbers and carrots; in several kinds of compot, in tomato juic ...1977857490
distinction between nuclear satellite dnas and chloroplast dna in higher plants.triton x-100 solubilized chloroplast dna but not nuclear dna from a mixture of chloroplasts and nuclei. the buoyant density of chloroplast dna was different from that of the satellite dna in all of the species examined (phaseolus coccineus, cucumis sativus, cucumis melo, antirrhinum majus, vicia faba, oenothera fruiticosa youngii). chloroplast dna constituted between 4.3% and 0.25% of the total leaf dna in these species, and was present as 5 to 20 copies in each chloroplast.197816660649
phytoparasitic nematodes adjacent to established strawberry plantations.plant-nematode populations associated with uncultivated vegetation, adjacent strawberry plants, and alternate crop sites were studied at three locations in minnesota. at one site (forest lake), paratylenchus projectus, meloidogyne hapla, and pratylenchus tenuis were frequently associated with the roots of native vegetation. these nematode species were also present in adjacent strawberry beds. among alternate crops observed, oats and muskmelon usually supported the fewest nematodes although moder ...197819305841
occurrence, isolation & characterisation of urease-inhibitor from melon (cucumis melo) seeds. 1978748169
light-dependent emission of hydrogen sulfide from plants.with the aid of a sulfur-specific flame photometric detector, an emission of volatile sulfur was detected from leaves of cucumber (cucumis sativus l.), squash and pumpkin (cucurbita pepo l.), cantaloupe (cucumis melo l.), corn (zea mays l.), soybean (glycine max [l.] merr.) and cotton (gossypium hirsutum l.). the emission was studied in detail in squash and pumpkin. it occurred following treatment of the roots of plants with sulfate and was markedly higher from either detached leaves treated via ...197816660257
a lectin from the exudate of the fruit of the vegetable marrow (cucurbita pepo) that has a specificity for beta-1,4-linked n-acetylglucosamine oligosaccharides.lectins are present in the exudate (presumably from the phloem) of the fruits of three species of the cucurbitaceae, namely vegetable marrow (cucurbita pepo), melon (cucumis melo) and cucumber (cucumis sativus). they are all strongly inhibited in their activities by chitin oligosaccharides, but only weakly by n-acetylglucosamine. glycopeptides from soya-bean agglutinin and fetuin are also strong inhibitors of cucurbita pepo lectin, indicating that it interacts with internal n-acetylglucosamine r ...1979534476
cell surfaces in plant-microorganism interactions: ii. evidence for the accumulation of hydroxyproline-rich glycoproteins in the cell wall of diseased plants as a defense mechanism.enrichment of the cell wall in hydroxyproline-rich glycoprotein is involved in the defense of muskmelon (cucumis melo) seedlings to colletotrichum lagenarium, the causative agent of anthracnose. the extent to which this accumulation proceeds may be experimentally modified by treating plants with ethylene or growing them in the presence of free l-trans-hydroxyproline. it appears that the increase in the wall hydroxyproline-rich glycoprotein mediated through ethylene is paralleled by an increasing ...197916660957
cell surfaces in plant-microorganism interactions: i. a structural investigation of cell wall hydroxyproline-rich glycoproteins which accumulate in fungus-infected plants.infection of muskmelon cucumis melo seedlings by the fungus colletotrichum lagenarium causes a 10-fold increase in the amount of cell wall hydroxyproline-rich glycoprotein. evidence for this increase was provided by studying two specific markers of this glycoprotein, namely hydroxyproline and glycosylated serine. the lability of the o-glycosidic linkage of wall-bound glycosylated serine in the presence of hydrazine, was used to determine the amount of serine which is glycosylated.a large increas ...197916660956
variations in the satellite dna content of cucumis melo in relation to dedifferentiation and hormone concentration.total dna from cucumis melo contains a 1.706 satellite dna which can be resolved into two components; one of these components has a higher temperature of melting (component i) then the other component ii). in this study, we have further investigated these components by thermal denaturation and by eco r1 digestion. component i reveals a homogeneous melting profile and is only partially cleaved by eco r1, whereas component ii reveals a heterogeneous melting profile and is entirely digested by eco ...19806253893
divergence, differential methylation and interspersion of melon satellite dna sequences.melon (cucumis melo) satellite dna consists of two components, q and s, each with a buoyant density in cscl of 1.707 g/ml, but differing by 9 degrees c in "melting" temperature. these physical properties appear to be in contradiction, since both depend on g + c content. in order to resolve this anomaly, base compositions were directly determined for isolated fractions. the low-"melting" component s contains 41.8% g + c, with 6% of c present as 5-methylcytosine, whereas q dna contains 54% g + c, ...19816172117
the mitochondrial genome is large and variable in a family of plants (cucurbitaceae).the genome sizes of mitochondrial dna from darkgrown (etiolated) shoots of several higher plants were determined by reassociation kinetics and restriction analysis. kinetic complexities obtained from reassociation kinetics measured spectrophotometrically indicate a mitochondrial genome size of 1600 md for muskmelon, 1000 md for cucumber, 560 md for zucchini squash and 220 md for watermelon (four species in the cucurbit family), as well as 240 md for pea and 320 md for corn. the kinetic curves al ...19816269758
enhancement of wound-induced ethylene synthesis by ethylene in preclimacteric cantaloupe.although intact fruits of unripe cantaloupe (cucumis melo l.) produce very little ethylene, a massive increase in ethylene production occurred in response to excision. the evidence indicates that this wound ethylene is produced from methionine via 1-aminocyclopropanecarboxylic acid (acc) as in ripening fruits. excision induced an increase in both acc synthase and the enzyme converting acc to ethylene. ethylene further increased the activity of the enzyme system converting acc to ethylene. the in ...198216662200
organization of highly repetitive satellite dna of two cucurbitaceae species (cucumis melo and cucumis sativus).the prominent satellites of the cucurbitaceae cucumis melo (melon) and cucumis sativus (cucumber) have been characterized, in actinomycin/cscl gradients where the satellite sequences can be separated from ribosomal, organelle, and main band dna the location of the satellites is different indicating a different gc content. the purified satellite of c. melo is cut by hindiii into a repeat unit of 380 bp; alui digestion gives rise to two bands (about 80 and 220 bp in size). the hindiii repeat unit ...19826278425
stereospecific conversion of 1-aminocyclopropanecarboxylic acid to ethylene by plant tissues : conversion of stereoisomers of 1-amino-2-ethylcyclopropanecarboxylic acid to 1-butene.inasmuch as the molecule of 1-aminocyclopropanecarboxylic acid (acc) possesses reflective symmetry but lacks rotational symmetry, the two chemically alike methylene groups can be distinguished by a stereospecific enzyme. to determine whether acc conversion to ethylene by plant tissues proceeds in a stereospecific fashion, the four stereoisomers of 1-amino-2-ethylcyclopropanecarboxylic acid (aec) were administered to postclimacteric apple (malus sylvestris mill., var. golden delicious), excised p ...198216662444
comparative morphology of eggs of heterophyids and clonorchis sinensis causing human infections in korea.in order to provide some clues for differential diagnosis of trematode infections in fecal examination, the comparative morphology of eggs of 5 kinds of heterophyid flukes (metagonimus yokogawai, heterophyes heterophyes nocens, heterophyopsis continua, stellantchasmus falcatus and pygidiopsis summa) and clonorchis sinensis was studied. the eggs were obtained from distal portion of uteri of worms which were recovered from men after treatment. the characteristic shape and apperance of each kind of ...198412891010
cell surfaces in plant-microorganism interactions : iv. fungal glycopeptides which elicit the synthesis of ethylene in plants.the production of ethylene by melon (cucumis melo cv cantaloup charentais) tissues is stimulated during incubation in the presence of fungal glycopeptides extracted from colletotrichum lagenarium, a pathogen of melon. these glycopeptides, called elicitors of ethylene, are found in the mycelium, the cell wall, and the culture filtrate. elicitation of ethylene is a relatively early phenomenon and lasts for several hours. upon purification of the crude elicitor extract by gel filtration and ion exc ...198416663747
identification of platelet inhibitor present in the melon (cucurbitacea cucumis melo).an active fraction was isolated from an aqueous melon extract (cucurbitacea cucumis melo) and was shown that it inhibits human platelet aggregation induced by epinephrine, adp, collagen, thrombin, sodium arachidonate, prostaglandin endoperoxide analogue u-46619 and paf-acether. identification of the active substance as adenosine was indicated by tlc which gave identical rf value compared to adenosine, by the uv spectrum, because the inhibitory effect on platelet aggregation disappeared after the ...19853931281
the cucumis plastome: physical map, intrageneric variation and phylogenetic relationships.a restriction map of the cucumis melo l. (melon) plastome was constructed by using several mapping approaches: single and double digestions of the chloroplast dna (chldna) with endonucleases (xhoi, smai, saci and pvuii) and hybridization to heterologous chldna probes and to isolated melon chldna fragments. four plastome-coded genes were located using heterologous probes. the overall organization and gene position of the melon plastome was found to be similar to that of tobacco and other angiospe ...198524247447
promotion by ethylene of the capability to convert 1-aminocyclopropane-1-carboxylic acid to ethylene in preclimacteric tomato and cantaloupe fruits.the intact fruits of preclimacteric tomato (lycopersicon esculentum mill) or cantaloupe (cucumis melo l.) produced very little ethylene and had low capability of converting 1-aminocyclopropane-1-carboxylic acid (acc) to ethylene. when these unripe tomato or cantaloupe fruits were treated with ethylene for 16 hours there was no increase in acc content or in ethylene production rate, but the tissue's capability to convert acc to ethylene increased markedly. such an effect was also observed in frui ...198516664067
mitochondrial gene expression in cucurbitaceae: conserved and variable features.we have examined mitochondrial dna (mtdna) sequence conservation, transcriptional patterns of mitochondrial genes, and mitochondrial translation products in four species of the plant family cucurbitaceae, in which there is a seven-fold range in mitochondrial genome size. a set of conserved mtdna sequences which we term "core" dna is present in all cucurbit genomes examined. in watermelon, only those mtdna restriction fragments which contain "core" dna hybridize with mtrna. similar numbers of pol ...19853870928
are sucrosyl-oligosaccharides synthesized in mesophyll protoplasts of mature leaves of cucumis melo?biosynthesis of sucrosyl-oligosaccharides (raffinose, stachyose) was traced in source leaves of cucumis melo after (14)c-photoassimilation. the main carbon compound exported was (14)c-labeled stachyose. no oligosaccharide synthesis was detected in young, importing leaves. mesophyll protoplasts, isolated from mature leaves which had previously photosynthesized (14)co2, did not contain (14)c-oligosaccharides but contained [(14)c]-sucrose and (14)c-hexoses. isolated minor-vein-enriched fractions fr ...198624232761
pathway of assimilate transfer between mesophyll cells and minor veins in leaves of cucumis melo l.photoassimilating mature leaves of cucumis melo exported carbon at a rate of 1.7 mg c·dm(-2)·h(-1). radiolabeling with (14)c showed that stachyose and raffinose are the main carbohydrates translocated. autoradiograms indicated that sieve elements of the abaxial phloem of minor veins are the sole conduits for carbon export from mature leaves and carbon import into immature leaflets. sieve elements of the abaxial phloem are associated with intermediary cells which are intimately connected with the ...198724227267
sucrose metabolism in netted muskmelon fruit during development.sugar content and composition are major criteria used in judging the quality of netted muskmelon (cucumis melo l. var reticulatus) fruit. sugar composition and four enzymes of sucrose metabolism were determined in ;magnum 45' muskmelon fruit at 10-day intervals beginning 10 days after pollination (dap) until full-slip (35 dap). sugar content increased in both outer (green) mesocarp and inner (orange) mesocarp between 20 and 30 dap. the major proportion of total increase in sugar was attributed t ...198716665448
the effects of fruit juices and fruits on the absorption of iron from a rice meal.the effects of the chemical composition of fruit juices and fruit on the absorption of iron from a rice (oryza sativa) meal were measured in 234 parous indian women, using the erythrocyte utilization of radioactive fe method. the corrected geometric mean fe absorptions with different juices varied between 0.040 and 0.129, with the variation correlating closely with the ascorbic acid contents of the juices (rs 0.838, p less than 0.01). ascorbic acid was not the only organic acid responsible for t ...19873593665
watermelon and ragweed share allergens.a biotin-avidin amplified elisa was used to measure antigen-specific ige for ragweed, representative members of the gourd family (watermelon, cantaloupe, honeydew melon, zucchini, and cucumber), and banana in the sera of 192 allergic patients, each with an ige greater than or equal to 180 microns/ml. sixty-three percent (120/192) of the sera contained antiragweed ige, and of these patients, 28% to 50% contained ige specific for any single gourd family member. in contrast, no greater than 11% of ...19873584742
effects of air quality on foliar injury, growth, yield, and quality of muskmelon.an evaluation of the effects of ambient ozone (o3) on muskmelon was conducted with the use of open-top chambers (otcs). 'superstar' muskmelons grown in charcoal-filtered (cf) chambers compared to those grown in nonfiltered (nf) chambers showed significant differences in the severity of visible foliar o3 injury. furthermore, plants grown in nf conditions had significantly less (21.3%) marketable fruit weight and fewer (20.9%) marketable fruit number than those from cf chambers. no differences wer ...198815092549
effect of temperature on infection and survival of rotylenchulus reniformis.from infestation of lettuce with preinfective females to egg deposition, populations of rotylenchulus reniformis from baton rouge, louisiana; lubbock and weslaco, texas; and mayaguez, puerto rico, required 41, 13, 7, and 7 days at 15, 20, 25, and 34 c, respectively. no nematode infection occurred at 10 c with any r. reniformis population, and the population from puerto rico did not reproduce at 15 c. nematode survival was not influenced by temperature, since populations from texas and louisiana ...198819290223
regeneration of plants from leaf explants of cucumis melo cv. pusa sharbati.leaves of three different sizes excised from 14, 21, 28 and 35-day-old seedlings of cucumis melo were cultured on a ms medium supplemented with a range and combination of growth regulators. maximum shoot differentiation from the leaf explants occurred in the combined presence of bap and 2ip at equimolar concentration of 1 μm. regeneration potential of leaves declined with increasing size of the leaves and the age of the donor seedlings. for elongation the shoots were transferred to ms+bap [1 μm] ...198824240267
nutrient salts promote light-induced degradation of indole-3-acetic acid in tissue culture media.the disappearance of indole-3-acetic acid (iaa) from cell-free liquid culture medium was followed in response to nutrient salts found in murashige-skoog salt base, light, and ph range of 4 to 7. the loss of iaa was accelerated by light or murashige-skoog salts. however, the combination of both light and murashige-skoog salts acted synergistically to catalyze the destruction of over 80% of the original iaa within 7 days of continuous incubation. under these same conditions, the loss of iaa was de ...198816666312
water relations of seed development and germination in muskmelon (cucumis melo l.) : i. water relations of seed and fruit development.total water potential (psi), solute potential, and turgor potential of field-grown muskmelon (cucumis melo l.) fruit tissue (pericarp) and seeds were determined by thermocouple psychrometry at 5-day intervals from 10 to 65 days after anthesis (daa). fruit maturity occurred between 44 and 49 daa, and seed germination ability developed between 35 and 45 daa. pericarp psi was essentially constant at approximately -0.75 megapascal (mpa) from 10 to 25 daa, then decreased to a minimum value of -1.89 m ...198816665921
sucrose phosphate synthase and acid invertase as determinants of sucrose concentration in developing muskmelon (cucumis melo l.) fruits.fruits of orange-fleshed and green-fleshed muskmelon (cucumis melo l.) were harvested at different times throughout development to evaluate changes in metabolism which lead to sucrose accumulation, and to determine the basis of differences in fruit sucrose accumulation among genotypes. concentrations of sucrose, raffinose saccharides, hexoses and starch, as well as activities of the sucrose metabolizing enzymes sucrose phosphate synthase (sps) (ec 2.4.1.14), sucrose synthase (ec 2.4.1.13), and a ...198916667212
variability and correlations in muskmelon in relation to the cultivation method.six fruit characters have been measured in 23 cultivars of cucumis melo, representing a wide geographical range. plants were grown both in the greenhouse and in the field. when the 23 cultivars were analyzed together, the largest component of variance was found between cultivars under both growth conditions, suggesting the existence of large genetic diversity for all the characters studied. generally, variance between plants within cultivars was less than or equal to variance between fruits with ...198924227250
in vitro plant regeneration from leaf and cotyledon explants of cucumis melo l.five different genotypes from in vitro as well as greenhouse grown melon plants were shown to be highly responsive for in vitro shoot formation from leaf explants when placed on basic ms medium supplemented with 1 mg/l 6-benzylaminopurine. in addition, a very suitable regeneration system was obtained when cotyledon pieces of mature seeds were incubated on the same culture medium. in this case, the first shoots already appeared after 10 days of incubation, and hundreds of shoots were formed on th ...198924240446
influence of glomus intraradices and soil phosphorus on meloidogyne incognita infecting cucumis melo.the interaction among glomus intraradices, meloidogyne incognita, and cantaloupe was studied at three soil phosphorus (p) levels in a greenhouse. all plants grew poorly in soil not amended with p, regardless of mycorrhizal or nematode status. in soil amended with 50 mug p /g soil, m. incognita suppressed the growth of nonmycorrhizal plants by 84%. in contrast, growth of mycorrhizal plants inoculated with m. incognita was retarded by only 21%. a similar trend occurred in plants grown in soil with ...198919287577
blood sugar lowering potentiality of selected cucurbitaceae plants of indian origin.using five experimental models, the blood sugar lowering efficacy of eight plants of cucurbitaceae family has been assessed. the ethanolic extract of cucumis sativus linn, cucumis melo utilissimum roxb, cucumis melo linn, benincasa hispida thunb cogn and tricosanthes anguina nees, when administered in 250 mg/kg dose, orally to rats failed to lower blood sugar or to depress the peak value, after glucose load. however, ethanolic extract of momordica charantia linn plant and coccinia indica whit an ...19892620957
[characterization of xanthomonas campestris pv. cucurbitae, causal agent of the bacterial spot of squash].bacterial leaf spot of squash was characterized for the first time in argentina. cultural, physiological, morphological and cross-infection tests on cucurbitaceae showed that the pathogen was xanthomonas campestris pv. cucurbitae (bryan) dye. the bacterium isolated from winter squash proved pathogenic for pumpkin, winter squash, cucumber and watermelon but no for muskmelon.19892748850
post-translational modifications in the large subunit of ribulose bisphosphate carboxylase/oxygenase.two adjacent n-terminal tryptic peptides of the large subunit of ribulose bisphosphate carboxylase/oxygenase [3-phospho-d-glycerate carboxy-lyase (dimerizing), ec 4.1.1.39] from spinach, wheat, tobacco, and muskmelon were removed by limited tryptic proteolysis. characterization by peptide sequencing, amino acid composition, and tandem mass spectrometry revealed that the n-terminal residue from the large subunit of the enzyme from each plant species was acetylated proline. the sequence of the pen ...19892928307
a simple purification of indole-3-acetic acid and abscisic acid for gc-sim-ms analysis by microfiltration of aqueous samples through nylon.a simple procedure was developed for the partial purification of plant tissue samples to be analyzed simultaneously for indole-3-acetic acid (iaa) and abscisic acid (aba). the procedure relies on removal of contaminants by filtration through nylon and partitioning into dichloromethane. this procedure successfully purified both iaa and aba from muskmelon, cotton, and broccoli tissue. twenty individual samples can be purified and methylated in 8 h for analysis of free iaa and aba with gas chromato ...198916666735
species determination of eggs of opisthorchiid and heterophyid flukes using scanning electron microscopy.eggs of opisthorchiid flukes have very characteristic muskmelon-like surface structure of their shells and can be differentiated using this mark from eggs of heterophyid flukes. surface structure and morphological details in operculum and abopercular end can be observed using scanning electron microscopy. eggs recorded very often in stool of laotians belong mostly to opisthorchis viverrini and sporadically to metagonimus yokogawai.19902337251
agrobacterium tumefaciens mediated transformation and regeneration of muskmelon plants.transgenic muskmelon (cucumis melo l.) plants were produced efficiently by inoculating cotyledon explants with agrobacterium tumefaciens strain lba4404 bearing a ti plasmid with the npt ii gene for kanaymcin resistance. after co-cultivation for three days, expiants were transferred to melon regeneration medium with kanamycin to select for transformed tissue. shoot regeneration occurred within 3-5 weeks; excised shoots were rooted on medium containing kanamycin before transferring to soil. morpho ...199024226603
abscisic acid alters the metabolism of indole-3-acetic acid in senescing flowers of cucumis melo l.experiments were conducted to investigate indole-3-acetic acid (iaa) and abscisic acid (aba) metabolism associated with postanthesis senescence of ovaries from nonpollinated muskmelon (cucumis melo l.) flowers. flowers attached to the vine were allowed to senesce for 4 days after anthesis or were harvested at full anthesis and aged for the same time interval with or without access to water. the iaa ester, amide-linked forms of iaa, free aba, and aba esters increased in senescent ovaries from flo ...199016667865
plant regeneration from cotyledon protoplasts of xinjiang muskmelon.cotyledon protoplasts were isolated from seedlings of xinjiang muskelon (cucumis melo var.saccharinus) grown under sterile conditions and cultured in modified miller medium. high frequency division of the protoplast-derived cells was observed. agarose bead culture with b6s3 tobacco crown gall nurse cells was found most suitable for the cotyledon protoplasts when compared with other culture methods. intact plants were regenerated from the protocalli by a two-step culture procedure with liquid and ...199024226702
water relations of seed development and germination in muskmelon (cucumis melo l.) : v. water relations of imbibition and germination.the initiation of radicle growth during seed germination may be driven by solute accumulation and increased turgor pressure, by cell wall relaxation, or by weakening of tissues surrounding the embryo. to investigate these possibilities, imbibition kinetics, water contents, and water (psi) and solute (psi(s)) potentials of intact muskmelon (cucumis melo l.) seeds, decoated seeds (testa removed, but a thin perisperm/endosperm envelope remains around the embryo), and isolated cotyledons and embryon ...199016667369
water relations of seed development and germination in muskmelon (cucumis melo l.) : iv. characteristics of the perisperm during seed development.we previously reported that an apparent water potential disequilibrium is maintained late in muskmelon (cucumis melo l.) seed development between the embryo and the surrounding fruit tissue (mesocarp). to further investigate the basis of this phenomenon, the permeability characteristics of the tissues surrounding muskmelon embryos (the mucilaginous endocarp, the testa, a 2- to 4-cell-layered perisperm and a single cell layer of endosperm) were examined from 20 to 65 days after anthesis (daa). wa ...199016667368
water relations of seed development and germination in muskmelon (cucumis melo l.) : iii. sensitivity of germination to water potential and abscisic acid during development.muskmelon (cucumis melo l.) seeds are germinable 15 to 20 days before fruit maturity and are held at relatively high water content within the fruit, yet little precocious germination is observed. to investigate two possible factors preventing precocious germination, the inhibitory effects of abscisic acid and osmoticum on muskmelon seed germination were determined throughout development. seeds were harvested at 5-day intervals from 30 to 65 days after anthesis (daa) and incubated either fresh or ...199016667367
distribution and immunolocalization of stachyose synthase in cucumis melo l.indirect evidence for the site of stachyose biosynthesis has been provided by determining the occurrence and distribution of stachyose, raffinose and galactinol, the donor of the galactosyl moiety for stachyose synthesis, in cucumis melo l. cv. ranjadew. studies of enzyme activities for the synthesis of these sugars and their distribution in different plant organs and isolates has led to the conclusion that stachyose is synthesized mainly in mature leaves and seeds. nevertheless, stachyose-synth ...199124186524
regulation of organogenesis and somatic embryogenesis by auxin in melon, cucumis melo l.various tissues of seeds and seedlings of melon were cultured in vitro to study the effects of auxin concentration on organogenesis and embryogenesis. adventitious shoots and somatic embryos were formed from explants of cotyledons of mature seeds, hypocotyls of seedlings, and leaves and petioles of young plantlets. expanded cotyledons of seedlings formed only adventitious shoots. all tissues responded similarly to the 2,4-d concentration in the media, that is, adventitious shoots were formed at ...199124221584
stachyose synthesis in mature leaves of cucumis melo. purification and characterization of stachyose synthase (ec 2.4.1.67).galactinol: raffinose-6-galactosyltransferase (ec 2.4.1.67), a stachyose synthase, was extracted from mature leaves of cucumis melo cv. ranjadew and was purified to homogeneity by (nh4)2so4 precipitation, ion-exchange chromatography, gel-filtration and non-denaturing polyacrylamide gel electrophoresis. a specific activity of 516 μkat · mg(-1) and a 160-fold purification was achieved. the ph optimum of the enzyme reaction was found to be 6.8 in sodium-phosphate buffer, and the temperature optimum ...199124194243
acid and neutral invertases in the mesocarp of developing muskmelon (cucumis melo l. cv prince) fruit.acid and neutral invertases were found in the mesocarp of developing muskmelon (cucumis melo l. cv prince) fruit and the activities of these enzymes declined with maturation of the fruit, concomitantly with the accumulation of sucrose. neutral invertase was only present in the soluble fraction and acid invertase was present in both the soluble and cell-wall fractions. the cell-wall fraction contained three types of acid invertase: a nacl-released invertase; an edta-released invertase, and a tigh ...199116668269
differential accumulation of hydroxyproline-rich glycoproteins in bean root nodule cells infected with a wild-type strain or a c4-dicarboxylic acid mutant of rhizobium leguminosarum bv. phaseoli.an antiserum raised against deglycosylated hydroxyproline-rich glycoproteins (hpgps) from melon (cucumis melo l.) was used to study the relationship between rhizobium infection and induction of hrgps in bean (phaseolus vulgaris l.) root nodule cells infected with either the wild-type or a c4-dicarboxylic acid mutant strain of rhizobium leguminosarum bv. phaseoli. in effective nodules, where fixation of atmospheric dinitrogen is taking place, hrgps were found to accumulate mainly in the walls of ...199124194237
eggshell morphology of the small eggs of human trematodes in thailand.light and scanning electron micrographs of opisthorchis viverrini, haplorchis taichui, h. pumilio, a phaneropsolus bonnei, and prosthodendrium molenkampi eggs were studied. under light microscopy. o. viverrini eggs had rough eggshells and prominent shoulders. h. taichui, h. pumilio were similar in shape and had smooth eggshells and prominent shoulders. h. pumilio eggs were bigger than h. taichui eggs. p. bonnei and p. molenkampi eggs had smooth eggshells and indistinct shoulders. p. bonnei eggs ...19911820654
temperature-sensitive replication of cucumber mosaic virus in muskmelon (cucumis melo cv. iroquois), maps to rna 1 of a slow strain.several strains of cucumber mosaic virus have been categorized as either 'fast' or 'slow', based on the time of appearance of symptoms after inoculation onto zucchini squash (curbita pepo cv. black beauty). these strains were examined for their ability to replicate in muskmelon (cucumis melo cv. iroquois) at elevated temperatures. all of the fast strains were able to replicate at 37 degrees c in muskmelon, whereas all of the slow strains were unable to replicate to detectable levels at 37 degree ...19911856702
toxicity of potassium cyanide added to fresh fruit and juice.to investigate the toxicity of potassium cyanide in fresh fruit and juice, male and female wistar rats were orally dosed with fruit homogenates or juices containing 3 x ld50 of potassium cyanide. these were given in single doses at various intervals after spiking. the dosing solutions were analysed for cyanide using a cyanide test kit. there was a good correlation between the toxic signs in rats and the cyanide remaining in dosing solutions. the toxicity of spiked apple and honeydew melon dimini ...19911959821
myrotoxins from a plant pathogenic isolate ofmyrothecium roridum.an isolate ofm roridum (atcc 52485) which is a potent pathogen to muskmelon was shown to produce a series of macrocyclic trichothecenes, the myrotoxins, heretofore found to be produced only by an. isolate ofm roridum which is a pathogen to tomato. the fact that these two isolates are virulent pathogens and both produce the same potent mycotoxins suggests that these novel trichothecenes may play an important role in the pathogenicity of the fungi.199123605653
tolerance to a whitefly-transmitted virus causing muskmelon yellows disease in spain.muskmelon yellowing disease was one of the most serious problems affecting muskmelon crops along the south-east coast of spain throughout the 1980s. the causal agent of this disease is a virus that we call muskmelon yellows virus (myv); myv is transmitted by the greenhouse whitefly trialeurodes vaporariorum westwood. it has proven impossible to find sources of resistance to myv within a wide collection of spanish muskmelon landraces and exotic varieties. however, 'nagata kin makuwa' and pi 16137 ...199224201359
partial characterization of the translational inhibitor present in seeds of cucumis melo l. 19921486983
induction of somatic embryogenesis and caulogenesis from cotyledon and leaf protoplast-derived colonies of melon (cucumis melo l.).a procedure leading to the regeneration of whole plants from protoplasts of melon is described. protoplasts were isolated from cotyledons and leaves of plants grown in vitro. after 14 days of culture, average viability and division rates were respectively 60% and 30% for the two organs, considering total initial protoplasts plated. the manipulation of the exogenous auxin / cytokinin balance in regeneration media enabled to direct morphogenesis towards somatic embryogenesis (1 mg·l(-1) 2,4-dichlo ...199224201728
the role of beta-galactosidases in the modification of cell wall components during muskmelon fruit ripening.the changes in activities of soluble beta-galactosidase and two forms of wall-bound beta-galactosidases extracted with nacl and edta were investigated throughout the development of muskmelon (cucumis melo l. cv prince) fruits. deae-cellulose ion-exchange chromatography of soluble beta-galactosidase revealed the presence of two isoforms. soluble isoform i was detected in all stages throughout the fruit development, whereas soluble isoform ii appeared around 34 d after anthesis when fruit ripening ...199216653123
amino acid sequences of trypsin inhibitors from oriental pickling melon (cucumis melo l. var. conomon makino) seeds.three inhibitors (cmcti-i, ii, and iii) were isolated from oriental pickling melon (cucumis melo l. var. conomon makino) seeds by acetone precipitation, gel filtration, and reversed phase chromatography. the amino acid sequences of these inhibitors were: [table; see text] the reactive sites (p1 and p1' sites) of these inhibitors are presumed to be the lys-ile indicated by an arrow, comparing them with other squash family inhibitors. all three inhibitors can inhibit lysyl endopeptidase and trypsi ...19921368838
patterns of assimilate production and translocation in muskmelon (cucumis melo l.) : ii. low temperature effects.muskmelon (cucumis melo l.) plants were exposed to a 10 degrees c chilling treatment for 72 hours, which induced leaf chilling injury symptoms (wilting, appearance of water-soaked areas, necrosis). chilling caused an accumulation of starch, sucrose, hexoses (glucose and fructose), and certain amino acids (glutamate, aspartate, and citrulline) in source leaf tissues, but no accumulation of stachyose or other galactosyl-oligosaccharides occurred. chilling also caused a general increase in sugar (s ...199216669026
patterns of assimilate production and translocation in muskmelon (cucumis melo l.) : i. diurnal patterns.continuous monitoring of steady-state carbon dioxide exchange rates in mature muskmelon (cucumis melo l.) leaves showed diurnal patterns of photosynthesis and respiration that were translated into distinct patterns of accumulation and phloem export of soluble sugars and amino acids. leaf soluble sugar patterns in general followed the pattern of photosynthetic activity observed in the leaf, whereas starch accumulated steadily throughout the light period. sugar and starch levels declined through t ...199216669025
evaluation of restriction fragment length polymorphism in cucumis melo.the objectives of this study were to assess the degree of restriction fragment length polymorphism (rflp) in cucumis melo and to determine interrelationships among cultivated varieties. initial screening of a genomic psti library revealed that approximately 40% of the clones were repetitive. a total of 162 unique and low-copy sequence clones were hybridized to seven diverse accesions of c. melo and a c. sativus cultivar 'pacer' to evaluate rflp variation. of these, 130 probes (80%) detected a po ...199224202522
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