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response of seedlings of a dwarf and a normal strain of watermelon to gibberellins.hypocotyl and root elongation in a dwarf and a normal strain of watermelon (citrullus lanatus [thunb.] matsu.) in the absence or presence of different gibberellins was investigated in seedlings grown under gold fluorescent light or in darkness. the normal strain, "sugar baby," responded only slightly to the gibberellic acids employed. at appropriate concentrations all of the gibberellic acids were capable of normalizing growth in the monorecessive dwarf strain, wb-2, in darkness or in light. gib ...197416658700
lipoxygenase and hydroperoxide lyase in germinating watermelon seedlings.lipoxygenase (ec 1.13.1.13) was found in seedlings of citrullus lanatus (thunb.) matsum. and nakai (watermelon). the enzyme has ph optima of 4.4 and 5.5 and is inhibited by 0.2 mm nordihydroguaiaretic acid. it is present in two functional units with estimated molecular weights of 120,000 and 240,000, respectively.a new enzyme, tentatively termed hydroperoxide lyase, has been partially purified from watermelon seedlings. the enzyme, located principally in the region of the hypocotyl-root junction ...197616659569
[beta-d-glucopyranosylstigmast-7-en-3beta-ol from citrullus lanatus var. citroides]. 1977859907
effects of gibberellic acid and gold light on germination, enzyme activities, and amino acid pool size in a dwarf strain of watermelon.gibberellic acid (ga(3)) promotes and continuous gold light inhibits germination of seeds of a dwarf strain (wb-2) of watermelon [citrullus lanatus (thunb.) matsu. and nakai]. osmotic inhibition of germination with mannitol in light-grown seeds of wb-2 was only slightly reversed by ga(3) at the concentrations used, whereas, ga(3) substantially relieved osmotic inhibition in dark-grown seeds.the effects of ga(3) and gold light on development of catalase and invertase activities and on levels of f ...197816660470
genetics of seed-coat colour in citrullus lanatus (thunb.) mansf.intervarietal crosses in watermelon, citrullus lanatus (thunb.) mansf., involving six parents with black (j18-1 and j 75), brown (j56-1 and n.h. midget), red (bykovski-199) or light cream (red nectar) seed-coat colour were made. parents, f1, f2 and backcross populations were evaluated for their phenotypic expressions with regard to the seed-coat colours involved. black colour was monogenically dominant over brown light cream and red colour of seed-coat separately or independently. red colour was ...197824311206
interaction of ethylene and a cytokinin in promoting hypocotyl elongation in a dwarf strain of watermelon.experiments were conducted to study the interaction of ethylene and the cytokinin n(6)-benzyladenine (ba) in promoting hypocotyl elongation in a dwarf strain of watermelon (citrullus lanatus [thunb] matsu. and nakai). optimum promotion of hypocotyl elongation is elicited by an apical treatment with 0.2 microgram ba. at dosages above 0.3 microgram per apex, ba-enhancement of elongation is reduced concomitant with stimulation of ethylene production and lateral expansion of hypocotyls. application ...198116662017
effect of water stress on the carbohydrate metabolism of citrullus lanatus seeds during germination.gluconeogenesis in citrullus lanatus seeds is a post germinative event. increases in isocitrate lyase activity and incorporation of radioactivity from [2-(14)c]acetate into sugars occur only after radicle emergence. during germination, the seeds appear to rely on carbohydrate as the respiratory substrate. at this time, glycolysis, the pentose phosphate pathway, and the tricarbocyclic acid cycle seem to be functional. utilization of raffinose during germination appears to be important.water stres ...198516664032
effects of allelopathic compounds of corn pollen on respiration and cell division of watermelon.the effects of the allelopathic compounds of an "'ethanolic extract" of corn pollen on growth, respiration, and cellular division of watermelon (citrullus lanatus, var. peacock improved) were studied. bioassays with the ethanolic extract showed an inhibition of radicle and hypocotyl growth. the effects on respiration were observed in isolated watermelon hypocotyl mitochondria. the ethanolic extract acts as an inhibitor of the electron pathway, decreasing oxygen consumption in state 3, with malat ...198824276995
control of pyrophosphated-fructose-6-phosphate 1-phosphotransferase activity in the cotyledons of citrullus lanatus.after initiation of radicle elongation, the pyrophosphate:d-fructose-6-phosphate 1-phosphotransferase (pfp) activity sharply increases in the cotyledons of citrullus lanatus. removal of the radicle early during incubation prevents the increase in pfp activity in the cotyledons evident in the control. removal of the radicle at any stage after germination results in a decrease in pfp activity in the cotyledons. application of kinetin (0.5 micromolar) or 2-chlorophosphonic acid (0.1 micromolar) to ...199016667523
pyrophosphate dependent phosphofructokinase of citrullus lanatus: molecular forms and expression of subunits.during germination and seedling establishment, the total pyrophosphate-dependent phosphofructokinase (pfp) activity in the cotyledons increases. two types of subunits with molecular weights of 68 (alpha-subunit) and 65 (beta-subunit) kilodaltons are present. the increase in activity coincides with an approximately 10-fold increase in beta-subunit and twofold increase in alpha-subunit content. different isoforms of pfp are present at all stages of incubation, but the ratio between the isoforms si ...199116668318
induction of pyrophosphate-dependent phosphofructokinase in watermelon (citrullus lanatus) cotyledons coincides with insufficient cytosolic d-fructose-1,6-bisphosphate 1-phosphohydrolase to sustain gluconeogenesis.during germination of citrullus lanatus, pyrophosphate-dependent phosphofructokinase (pfp) activity is induced. the peak of pfp activity coincides with the maximum gluconeogenic flux and high fructose-2,6-bisphosphate (fru-2,6-p2) concentrations. determination of cytosolic fructose-1,6 bisphosphatase (fbpase) activity in crude extracts is unreliable because of the high pfp activity. the fbpase activity, after correction for the contaminating pfp, is only one-third of the pfp activity. purified c ...199312231792
somatic embryogenesis and plant regeneration from immature cotyledons of watermelon.cotyledon expiants from immature embryos of five watermelon [citrullus lanatus (thunb.)matsum. & nakai] genotypes were incubated in the dark for three weeks on a modified ms medium containing b5 vitamins, 2,4-d (10, 20 or 40μm), 0.5 μm of either ba or tdz, and 7 g·1(-1) tc agar. somatic embryos, some with well developed cotyledons, were observed on cotyledon expiants three to four weeks after transfer to ms medium without pgrs and 16h photoperiod. the best pgr combination for somatic embryogenes ...199324202069
physiological effects of ozone on cultivars of watermelon (citrullus lanatus) and muskmelon (cucumis melo) widely grown in spain.two cultivars of watermelon (citrullus lanatus) and muskmelon (cucumis melo), which are widely grown in spain, were exposed to ozone (70 nl litre(-1), 6 h d(-1)) for 21 days. ozone sensitivity was assessed by recording the extent of visible injury, changes in fast-fluorescence kinetics, the relative-growth rate (r) of root (rr) and shoot (rs), and effects on the number of flowers produced per plant. leaf gas exchange was measured in order to provide some indication of the factors underlying diff ...199315091805
production of an anti-allergic triterpene bryonolic acid, by plant cell cultures.cell suspension cultures of luffa cylindrica, citrullus lanatus, and related cucurbitaceous plants accumulate large quantities of bryonolic acid (3 beta-hydroxy-d:c-friedoolean-8-en-29-oic acid) [1], an acidic, pentacyclic triterpene found exclusively in the roots of the intact plants. this compound could readily be isolated from cultured cells with chcl3 and purified simply by recrystallization. pharmacological tests using mice demonstrated that bryonolic acid or its derivative is active agains ...19938463793
genetic transformation and plant regeneration of watermelon using agrobacterium tumefaciens.adventitious shoots formed on the proximal cut edges of different cotyledonary explants of watermelon [citrullus lanatus (thunb.) matsum. & nakai; cvs. sweet gem and gold medal] cultured on murashige and skoog's (ms) medium with 1 mgl(-1) 6-benzyladenine (ba). light (16-h photoperiod, about 7 wm(-2) cool-white fluorescent lamps) was essential for shoot formation. to obtain transformed plants, cotyledonary explants of 'sweet gem' were cocultured with agrobacterium tumefaciens lba4404, a disarmed ...199424193834
reaction of ten cultivars of watermelon (citrullus lanatus) to a puerto rican population of meloidogyne incognita.ten cultivars of watermelon were evaluated for their response to a puerto rican population of meloidogyne incognita under greenhouse conditions in a 2-year study (1989 and 1990). ten-day-old seedlings were planted in steam-sterilized soil in 15-cm-d plastic pots. the nematode inoculum consisted of 10,000 eggs and (or) second-stage juveniles (j2)/plant. the cultivars were sugar baby, charleston gray, seedless, prince charles, charleston 76, jubilee, florida giant, royal charleston, royal sweet, a ...199419279940
simple sequence repeats in watermelon (citrullus lanatus (thunb.) matsum. & nakai).simple sequence repeat length polymorphisms were utilized to examine genetic relatedness among accessions of watermelon (citrullus lanatus (thunb.) matsum. & nakai). a size-fractionated taqi genomic library was screened for the occurrence of dimer and trimer simple sequence repeats (ssrs). a total of 96 (0.53%) ssr-bearing clones were identified and the inserts from 50 of these were sequenced. the dinucleotide repeats (ct)n and (ga)n accounted for 82% of the ssrs sequenced. pcr primer pairs flan ...19979276932
studies on toxigenic fungi in roasted foodstuff (salted seed) and halotolerant activity of emodin-producing aspergillus wentii.commercial roasted salted peanuts (3% nacl), popcorn (1% nacl), summer-squash (9% nacl), sunflower (3% nacl) and wild-melon (3% nacl) seeds are polluted with fungi, mostly aspergillus flavus, a. niger, penicillium chrysogenum, p. corylophilum and rhizopus stolonifer. contamination of popcorn with the fungi is about 10 times higher than in the other foods. these fungi, common also on unsalted seeds, are significantly inhibited in seeds (30% moisture content) treated with 9-21% nacl. the halotoler ...19989821293
biochemical analysis of plant protection afforded by a nonpathogenic endophytic mutant of colletotrichum magnaa nonpathogenic mutant of colletotrichum magna (path-1) was previously shown to protect watermelon (citrullus lanatus) and cucumber (cucumis sativus) seedlings from anthracnose disease elicited by wild-type c. magna. disease protection was observed in stems of path-1-colonized cucurbits but not in cotyledons, indicating that path-1 conferred tissue-specific and/or localized protection. plant biochemical indicators of a localized and systemic (peroxidase, phenylalanine ammonia-lyase, lignin, and ...19999952476
cloning and molecular analyses of a gibberellin 20-oxidase gene expressed specifically in developing seeds of watermelon.to understand the biosynthesis and functional role of gibberellins (gas) in developing seeds, we isolated cv20ox, a cdna clone from watermelon (citrullus lanatus) that shows significant amino acid homology with ga 20-oxidases. the complementary dna clone was expressed in escherichia coli as a fusion protein, which oxidized ga(12) at c-20 to the c(19) compound ga(9), a precursor of bioactive gas. rna-blot analysis showed that the cv20ox gene was expressed specifically in developing seeds. the gen ...199910517828
mechanisms of action and dose-response relationships governing biological control of fusarium wilt of tomato by nonpathogenic fusarium spp.abstract three isolates of nonpathogenic fusarium spp. (cs-1, cs-20, and fo47), previously shown to reduce the incidence of fusarium wilt diseases of multiple crops, were evaluated to determine their mechanisms of action and antagonist-pathogen inoculum density relationships. competition for nutrients, as represented by a reduction in pathogen saprophytic growth in the presence of the biocontrol isolates, was observed to be an important mechanism of action for isolate fo47, but not for isolates ...199918944639
rapid high-performance liquid chromatography method to quantitate elaterinide in juice and reconstituted residues from a bitter mutant of hawkesbury watermelon.a c(18) reversed-phase hplc method has been developed to analyze the concentration of 2-o-beta-d-glycopyranosyl-cucurbitacin e (synonym, elaterinide) in juice or reconstituted residues of juice derived from a bitter mutant of hawkesbury watermelon, citrullus lanatus (thunb.) matsum. & nakai. the method requires only two steps (homogenation and filtration) for sample preparation prior to column injection and, thus, is amenable to processing large numbers of test samples. percent elaterinide in ju ...199910552560
[studies of molecular marker-assisted-selection for resistance to fusarium wilt in watermelon (citrullus lanatus) breeding].the rapd marker opp01/700 proved to be linked to the fusarium wilt resistant gene in watermelon wild germplasm pi296341 was cloned and sequenced, and transferred into a scar marker. the linked marker was tested to be one copy by southern blotting. the technique of detection of the scar-amplified products was simplified. these techniques were applied in selecting resistant plants in f3 of the introgression of the disease resistant gene. it is the first report in this field of transfer ring a rapd ...200010887683
determination of the rate of photoreduction of o2 in the water-water cycle in watermelon leaves and enhancement of the rate by limitation of photosynthesis.a study was performed to determine how the electron fluxes for the photosynthetic carbon reduction (pcr) and the photorespiratory carbon oxidation (pco) cycles affect the photoreduction of o2 at psi, which is the limiting step in the water-water cycle. simultaneous measurements were made of co2-gas exchange, transpiration and quantum yield of psii [phi(psii)] using leaves of watermelon (citrullus lanatus). the total electron flux in psii[je(psii)], as estimated from phi(psii), was always larger ...200010805597
regulation of oxylipin synthesis.two very common groups of oxylipins formed in plants involve the conversion of fatty acid hydroperoxides, such as hydroperoxy-octadecatrienoic acid, into further metabolites by allene oxide synthase and hydroperoxide lyase. both of these oxylipin branch pathways appear to be ubiquitous or nearly so in plants, but the relative activities of these two branches vary among plant species. in most plants examined, including arabidopsis, product formation from either of these pathways is minimal until ...200011171228
[the ultrastructural study of synergids before and after fertilization in watermelon embryo sacs].the ultrastructure of synergids of watermelon (citrullus lanatus l.) was investigated using transmission electron microscopy at following stages of embryo sacs: 1. unpollination, on the first flowering day. 2. unpollination, on 2nd day after anthesis (daa). 3. fertilization, on daa 2. the synergids with distinct filiform apparatus at the micropylar end have abundant organelle, such as mitochondria, endoplasmic reticulum, and plastids in cytoplasm, which indicate that they are active on the first ...200112549210
pharmacognostical stucies on the seeds of mulam citrullus lanatus (thunb.) mats & nakai (cucurbitacae).in the siddha system of medicine cotyledons of the seeds of c. lanatus are known as mulam/pullum/pitcha (tamil) and used as pulukolli (vermifuge), karpa moolikai (general tonic) and as aanmeiperukki (aphrodisiac). in the ayurvedic system of medicine seeds are said to have properties like sheeta (cooling), mootrala (diuretic) and vrshya (aphrodisiac). the present paper deals with macro and microscopical studies, maceration, histochemical tests, solubility, physical contents, extractive values, te ...200122557025
resistance to cold and heat stress: accumulation of phenolic compounds in tomato and watermelon plants.tomato plants, lycopersicon esculentum l. cv. tmknvf(2), and watermelon plants, citrullus lanatus [thomb.] mansf. cv. dulce maravilla, were grown for 30 days at different temperatures (15, 25 and 35 degrees c). we analysed soluble phenolics, enzymatic activities (phenylalanine ammonia-lyase, polyphenol oxidase and peroxidase), and dry weight. the impact of the three temperatures was different in tomato and watermelon. our results indicate that heat stress in tomato plants occurred at 35 degrees ...200111164603
resistance of polish lines and hybrids of watermelon [citrullus lanatus (thunb.) matsum et nakai] to fusarium oxysporum at the seedling stage.watermelon is a species cultivated in the hot climate or in the greenhouse. since recently it has also started to be grown in the open in the polish climate. this species is frequently at risk of fusarium oxysporum infection. between 1996 and 1997 ten inbred lines and nine hybrids of polish origin were tested for resistance to this pathogen. the test was conducted with the use of four isolates of f. oxysporum: three from polish infected plants (formae speciales not determined), while the fourth ...200212080172
crop pollination from native bees at risk from agricultural intensification.ecosystem services are critical to human survival; in selected cases, maintaining these services provides a powerful argument for conserving biodiversity. yet, the ecological and economic underpinnings of most services are poorly understood, impeding their conservation and management. for centuries, farmers have imported colonies of european honey bees (apis mellifera) to fields and orchards for pollination services. these colonies are becoming increasingly scarce, however, because of diseases, ...200212486221
a genetic linkage map for watermelon derived from a testcross population: ( citrullus lanatus var. citroides x c. lanatus var. lanatus) x citrullus colocynthis.a genetic linkage map was constructed for watermelon using a testcross population [plant accession griffin 14113 ( citrullus lanatus var. citroides) x new hampshire midget (nhm; c. lanatus var. lanatus)] x u.s. plant introduction (pi) 386015 ( citrullus colocynthis). the map contains 141 randomly amplified polymorphic dna (rapd) markers produced by 78 primers, 27 inter-simple sequence repeat (issr) markers produced by 17 primers, and a sequence-characterized amplified region (scar) marker that w ...200212582504
the expression of the saccharomyces cerevisiae hal1 gene increases salt tolerance in transgenic watermelon [citrullus lanatus (thunb.) matsun. & nakai.].an optimised agrobacterium-mediated gene transfer protocol was developed in order to obtain watermelon transgenic plants [citrullus lanatus (thunb.) matsun. & nakai.]. transformation efficiencies ranged from 2.8% to 5.3%, depending on the cultivar. the method was applied to obtain genetically engineered watermelon plants expressing the saccharomyces cerevisiae hal1 gene related to salt tolerance. in order to enhance its constitutive expression in plants, the hal1 gene was cloned in a pbin19 plas ...200312783167
influence of temperature on biomass, iron metabolism and some related bioindicators in tomato and watermelon plants.tomato, lycopersicon esculentum l. cv. rx-335, and watermelon plants, citrullus lanatus [thom.] mansf. cv. f-90 were grown under controlled conditions at three different temperatures (10 degrees, 25 degrees and 35 degrees c) for 30 days. the aim of the experiment was to analyse the effect of the different temperatures on fe uptake and distribution, as well as the behaviour of the main bioindicators of this element. thus, we analysed the total and free fe concentrations and h2o2 concentrations, a ...200314593808
construction of a linkage map and qtl analysis of horticultural traits for watermelon [citrullus lanatus (thunb.) matsum & nakai] using rapd, rflp and issr markers.we have been constructing linkage maps for watermelon ( citrullus lanatus) on the basis of random amplified polymorphic dna (rapd), restriction fragment length polymorphism (rflp), inter-simple sequence repeats (issrs) and isozymes using an f(2) population derived from a crossing between a cultivated inbred line (h-7; c. lanatus) and an african wild form (sa-1; c. lanatus). a total of 120 f(2) plants was used for construction of a linkage map using 477 rapds, 53 rflps, 23 issrs and one isozyme m ...200312647050
ethylene-induced gene expression, enzyme activities, and water soaking in immature and ripe watermelon (citrullus lanatus) fruit.watermelon fruit exhibit acute softening and placental-tissue water soaking following short exposure to exogenous ethylene. experiments were performed to address transcript abundance and activities of cell wall and membrane hydrolases in placental tissue in response to treatment of watermelon fruit with ethylene. watermelon fruit were harvested at immature and full-ripe stages and exposed to 50 microl l(-1) ethylene for 6 days at 20 degrees c. ethylene affected the abundance of transcripts for p ...200415128025
induction of water-soaking deterioration in watermelon fruit by ethylene.watermelon (citrullus lanatus thunb. mansfeld) fruit were held in 50 microl/l of ethylene at 20 degrees c following 18 h application with 5 microl/l of 1-methylcyclopropene (1-mcp) to investigate the involvement of ethylene in the regulation of water-soaking. the early responses of watermelon fruit to ethylene were increased electrolyte leakage and free juice content, accompanying softening and water-soaking of placental tissue. early evidence of water-soaking appeared in the inner pericarp near ...200415599024
sequence variation at cpdna regions of watermelon and related wild species: implications for the evolution of citrullus haplotypes.sequencing analysis of one coding and four noncoding cpdna regions was conducted to infer biogeographic and evolutionary relationships in the genus citrullus. eighteen taxa from diverse geographical areas were included. a low number of parsimony informative characters (1.1%) was observed at the ∼4 kb section of cpdna. variability within citrullus was detected primarily at noncoding regions of high a + t content. substitution rates varied from 0-0.48% for ndhf with a + t content of 68.4% to 0.39- ...200421652338
a pisum sativum glyoxysomal malate dehydrogenase induced by cadmium exposure.the glyoxysomal malate dehydrogenase (gmdh) catalyses the formation of oxaloacetate from malate during beta-oxidation of fatty acids in the glyoxysome. a partial pisum sativum l. (cv. greenfeast) cdna was first isolated from a suppression subtractive hybridisation cdna library obtained from heavy metal stressed plants. the full length cdna was then isolated by rapid amplification of cdna ends. the translated sequence showed strong similarity to cucumis sativus and citrullus lanatus gmdh includin ...200415497444
potent hydroxyl radical-scavenging activity of drought-induced type-2 metallothionein in wild watermelon.wild watermelon (citrullus lanatus sp.) has the ability to tolerate severe drought/high light stress conditions despite carrying out normal c3-type photosynthesis. here, mrna differential display was employed to isolate drought-responsive genes in the leaves of wild watermelon. one of the isolated genes, clmt2, shared significant homology with type-2 metallothionein (mt) sequences from other plants. the second-order rate constant for the reaction between a recombinant clmt2 protein and hydroxyl ...200415351703
plant er genes that encode photorespiratory enzymes confer resistance against disease.downy mildew caused by the oomycete pathogen pseudoperonospora cubensis is a devastating foliar disease of cucurbits worldwide. we previously demonstrated that the wild melon line pi 124111f (pi) is highly resistant to all pathotypes of p. cubensis. that resistance was controlled genetically by two partially dominant, complementary loci. here, we show that unlike other plant disease resistance genes, which confer an ability to resist infection by pathogens expressing corresponding avirulence gen ...200414688292
inheritance of resistance to zucchini yellow mosaic virus and watermelon mosaic virus in watermelon.high resistance to zucchini yellow mosaic virus-china strain (zymv-ch) and moderate resistance to watermelon mosaic virus (wmv) were found in a selection of pi 595203 (citrullus lanatus var. lanatus), an egusi type originally collected in nigeria. mixed inoculations showed primarily that these two viruses have no cross-protection. this fact may explain the high frequency of mixed infection often observed in commercial fields. when plants were inoculated with a mixture of the two viruses, the fre ...200415475395
watermelon (citrullus lanatus) hydroperoxide lyase greatly increases c6 aldehyde formation in transgenic leaves.fatty acid hydroperoxide lyase (hl) is the key enzyme for the production of the "green note"compounds, leaf aldehyde [(2e)-hexenal] and leaf alcohol [(3z)-hexenol], in plant tissues. a cdna encoding hl was cloned from leaves of watermelon (citrullus lanatus) and expressed in nicotiana tabacum. the enzyme is 3 times more active with 13-hydroperoxylinolenic acid than with 13-hydroperoxylinoleic acid. the activity against 9-hydroperoxides of polyunsaturated fatty acids is minimal. enzyme activity o ...200515769134
co-expression of cytochrome b561 and ascorbate oxidase in leaves of wild watermelon under drought and high light conditions.despite carrying out c3 photosynthesis, wild watermelon (citrullus lanatus sp.) exhibits exceedingly good tolerance to severe drought at high light intensities. however, the mechanism(s) by which this plant protects itself from photodamage has yet to be elucidated. in this study, we characterized wild watermelon cytochrome b561 (cyt b561), which potentially mediates regeneration of apoplastic ascorbate by transferring electrons from cytosolic ascorbate across the plasma membrane. two cdna specie ...200516020428
qualitative inheritance of rind pattern and flesh color in watermelon.watermelon [citrullus lanatus (thunb.) matsum. & nakai var. lanatus] is a diverse species, with fruits of different sizes, shapes, rind patterns, and flesh colors. this study measured the inheritance of novel rind phenotypes and verified the genetics of white, red, salmon yellow, and canary yellow flesh colors. for each of the 11 crosses, six generations (p(a)s1, p(b)s1, f1, f2, bc1p(a), and bc1p(b)) were produced to form 11 families. three new genes were identified and designated as follows: sc ...200616489140
inheritance of egusi seed type in watermelon.an unusual seed mutant in watermelon (citrullus lanatus var. lanatus) has seeds with a fleshy pericarp, commonly called egusi seeds. the origin of the phenotype is unknown, but it is widely cultivated in nigeria for the high protein and carbohydrate content of the edible seeds. egusi seeds have a thick, fleshy pericarp that appears during the second to third week of fruit development. we studied the inheritance of this phenotype in crosses of normal seeded charleston gray and calhoun gray with t ...200615220396
analysis of gamma-irradiated melon, pumpkin, and sunflower seeds by electron paramagnetic resonance spectroscopy and gas chromatography-mass spectrometry.seeds of melon (citrullus lanatus var. sp.), pumpkin (cucurbita moschata), and sunflower (heliantus annus) were gamma-irradiated at 1, 3, 5, and 10 kgy and analyzed by electron paramagnetic resonance (epr) and gas chromatography-mass spectrometry (gc-ms) according to en1787:2000 and en1785:2003, respectively. distinguishable triplet signals due to the presence of induced cellulose radicals were found at 2.0010-2.0047 g in the epr spectra. the gamma-irradiated radiolytic markers of 2-dodecylcyclo ...200616968077
spatial pattern and sequential sampling of squash bug (heteroptera: coreidae) adults in watermelon.spatial distribution patterns of adult squash bugs were determined in watermelon, citrullus lanatus (thunberg) matsumura and nakai, during 2001 and 2002. results of analysis using taylor's power law regression model indicated that squash bugs were aggregated in watermelon. taylor's power law provided a good fit with r2 = 0.94. a fixed precision sequential sampling plan was developed for estimating adult squash bug density at fixed precision levels in watermelon. the plan was tested using a resam ...200616686160
construction of a watermelon bac library and identification of ssrs anchored to melon or arabidopsis genomes.a bacterial artificial chromosome (bac) library was constructed for watermelon (citrullus lanatus (thunb.) matsum. & nakai var. lanatus) with an average insert-size of 106 kb, providing 21 haploid genome equivalents. the library was used to identify bac clones that are anchored to probes evenly distributed on the genomes of melon or arabidopsis. twenty eight probes (representing 66% of the tested probes) from melon and 30 probes (65%) from arabidopsis identified positive bac clones. two methods ...200616604337
molecular phylogeny of cucumis species as revealed by consensus chloroplast ssr marker length and sequence variation.to investigate phylogenetic relationships in the genus cucumis, 9 consensus chloroplast simple sequence repeat (ccssr) primer pairs (ccssr3, 9, 11, 13, 14, 17, 20, 21, and 23) were employed for dna fragment length variation and 5 amplified fragments, ccssr4, 12, 13, 19, and 20, were sequenced using total dna from 13 accessions representing 7 african cucumis species (x = 12), 3 cucumis melo l. (x = 12) accessions, 2 cucumis sativus l. (x = 7) accessions, and 1 cucumis hystrix chakr. (x = 12) acce ...200616604104
the cucurbit images (1515-1518) of the villa farnesina, rome.the gorgeous frescoes organized by the master renaissance painter raphael sanzio (1483-1520) and illustrating the heavenly adventures of cupid and psyche were painted between 1515 and 1518 to decorate the roman villa (now known as the villa farnesina) of the wealthy sienese banker agostino chigi (1466-1520). surrounding these paintings are festoons of fruits, vegetables and flowers painted by giovanni martini da udine (1487-1564), which include over 170 species of plants. a deconstruction and co ...200616314340
field captures of wild melon fly (diptera: tephritidae) with an improved male attractant, raspberry ketone formate.field-trapping evaluations of the new male attractant, formic acid 4-(3-oxobutyl) phenyl ester (raspberry ketone formate [rkf]) were conducted in hawaii with wild populations of melon flies, bactrocera cucurbitae coquillett (diptera: tephritidae), to determine its activity in the field and to evaluate new plastic matrix formulations. all tests were compared with the standard melon fly attractant 4-(4-acetoxyphenyl) -2-butanone (cuelure [cl]), which is the attractant of choice for detection progr ...200717849860
peroxisomal membrane manganese superoxide dismutase: characterization of the isozyme from watermelon (citrullus lanatus schrad.) cotyledons.in this work the manganese superoxide dismutase (mn-sod) bound to peroxisomal membranes of watermelon cotyledons (citrullus lanatus schrad.) was purified to homogeneity and some of its molecular properties were determined. the stepwise purification procedure consisted of ammonium sulphate fractionation, batch anion-exchange chromatography, and anion-exchange and gel-filtration column chromatography using a fast protein liquid chromatography system. peroxisomal membrane mn-sod (permn-sod; ec 1.15 ...200717545229
rapid method for total carotenoid detection in canary yellow-fleshed watermelon.yellow-fleshed watermelons (citrullus lanatus[thunb.] matsum. and nakai) contain many different carotenoids, all in low to trace amounts. since there is not 1 predominant carotenoid in yellow-fleshed watermelon, testing the total carotenoid content among watermelon lines is important in determining the antioxidant potential and thus potential health benefits of different varieties. unfortunately, current methods to assay total carotenoid content are time consuming and require organic solvents. t ...200717995749
assessment of attractiveness of plants as roosting sites for the melon fly, bactrocera cucurbitae, and oriental fruit fly, bactrocera dorsalis.the use of toxic protein bait sprays to suppress melon fly, bactrocera cucurbitae (coquillett) (diptera: tephritidae), populations typically involves application to vegetation bordering agricultural host areas where the adults seek shelter ("roost"). although bait spray applications for suppression of oriental fruit fly, bactrocera dorsalis (hendel), populations have traditionally been applied to the host crop, rather than to crop borders, roosting by oriental fruit flies in borders of some crop ...200720334596
the cucurbits of mediterranean antiquity: identification of taxa from ancient images and descriptions.a critical analysis was made of cucurbit descriptions in dioscorides' de materia medica, columella's de re rustica and pliny's historia naturalis, works on medicine, agriculture and natural science of the 1st century ce, as well as the mishna and tosefta, compilations of rabbinic law derived from the same time period together with cucurbit images dating from antiquity including paintings, mosaics and sculpture. the goal was to identify taxonomically the mediterranean cucurbits at the time of the ...200717932073
acc synthase genes are polymorphic in watermelon (citrullus spp.) and differentially expressed in flowers and in response to auxin and gibberellin.the flowering pattern of watermelon species (citrullus spp.) is either monoecious or andromonoecious. ethylene is known to play a critical role in floral sex determination of cucurbit species. in contrast to its feminizing effect in cucumber and melon, in watermelon ethylene promotes male flower development. in cucumber, the rate-limiting enzyme of ethylene biosynthesis, 1-aminocyclopropane-1-carboxylate (acc) synthase (acs), regulates unisexual flower development. to investigate the role of eth ...200818367517
recovery from cucurbit leaf crumple virus (family geminiviridae, genus begomovirus) infection is an adaptive antiviral response associated with changes in viral small rnas.a strong recovery response occurs in cantaloupe (cucumis melo) and watermelon (citrullus lanatus) infected with the bipartite begomovirus cucurbit leaf crumple virus (culcrv). this response is characterized by initially severe symptoms, which gradually become attenuated (almost symptomless). an inverse relationship was detected between viral dna levels and recovery, indicating that recovered tissues had reduced viral titers. recovered tissues also were resistant to reinfection with culcrv; i.e., ...200818943741
quality evaluation and prediction of citrullus lanatus by 1h nmr-based metabolomics and multivariate analysis.(1)h nmr spectrometry in combination with multivariate analysis was considered to provide greater information on quality assessment over an ordinary sensory testing method due to its high reliability and high accuracy. the sensory quality evaluation of watermelon (citrullus lanatus (thunb.) matsum. & nakai) was carried out by means of (1)h nmr-based metabolomics. multivariate analyses by partial least-squares projections to latent structures-discrimination analysis (pls-da) and pls-regression of ...200818588311
gene expression in developing watermelon fruit.cultivated watermelon form large fruits that are highly variable in size, shape, color, and content, yet have extremely narrow genetic diversity. whereas a plethora of genes involved in cell wall metabolism, ethylene biosynthesis, fruit softening, and secondary metabolism during fruit development and ripening have been identified in other plant species, little is known of the genes involved in these processes in watermelon. a microarray and quantitative real-time pcr-based study was conducted in ...200818534026
analysis of erythritol in foods by polyclonal antibody-based indirect competitive elisa.sugar alcohols are widely used as food additives and drug excipients. erythritol (ins 968) is an important four-carbon sugar alcohol in the food industry. erythritol occurs naturally in certain fruits, vegetables, and fermented foods. currently, hplc and gc methods are in use for the quantification of erythritol in natural/processed foods. however, an immunoassay for erythritol has not been developed so far. we have utilized affinity-purified erythritol-specific antibodies generated earlier [9] ...200818369603
programmed proteome response for drought avoidance/tolerance in the root of a c(3) xerophyte (wild watermelon) under water deficits.water availability is a critical determinant for the growth and ecological distribution of terrestrial plants. although some xerophytes are unique regarding their highly developed root architecture and the successful adaptation to arid environments, virtually nothing is known about the molecular mechanisms underlying this adaptation. here, we report physiological and molecular responses of wild watermelon (citrullus lanatus sp.), which exhibits extraordinarily high drought resistance. at the ear ...200818178965
carotenoid content and in vitro bioaccessibility of lycopene from guava (psidium guajava) and watermelon (citrullus lanatus) by high-performance liquid chromatography diode array detection.the carotenoid content and in vitro accessibility of the 'sugar baby' variety of watermelon and the 'horana red' variety of guava from sri lanka was determined using high-performance liquid chromatography. the high-performance liquid chromatography chromatogram showed that the guava 'horana red' variety contained almost exclusively lycopene (45.3 +/- 8.0 microg/g fresh weight (fw)), with a small amount of lutein (2.1 +/- 0.6 microg/g fw), beta-carotene (2.0 +/- 0.2 microg/g fw) and beta-cryptoxa ...200919817635
[analysis of the level and pattern of genomic dna methylation in different ploidy watermelons by msap (citrullus lanatus)].dna methylation is one of the major epigenetic modifications. it is very important to the regulation of gene expression. in present study, an autoploidy series (2x, 3x and 4x) in watermelon (citrullus lanatus) was constructed and msap (methylation-sensitive amplification polymorphism) analysis was conducted to elucidate the level and pattern of dna methylation at ccgg sites in different ploidy watermelons. totally, 1883 genetic loci were produced by 23 pairs of selective primers, of which 647, 6 ...200919537195
the cucurbitaceae and solanaceae illustrated in medieval manuscripts known as the tacuinum sanitatis.beginning in the last two decades of the 14th century, richly illuminated versions of the tacuinum sanitatis, the latin translation of an 11th-century arabic manuscript known as taqwim al-sihha bi al-ashab al-sitta, were produced in northern italy. these illustrated manuscripts provide a window on late medieval life in that region by containing some 200 full-page illustrations, many of which vividly depict the harvest of vegetables, fruits, flowers, grains, aromatics and medicinal plants. our ob ...200919318382
non-synonymous single nucleotide polymorphisms in the watermelon eif4e gene are closely associated with resistance to zucchini yellow mosaic virus.zucchini yellow mosaic virus (zymv) is one of the most economically important potyviruses infecting cucurbit crops worldwide. using a candidate gene approach, we cloned and sequenced eif4e and eif(iso)4e gene segments in watermelon. analysis of the nucleotide sequences between the zymv-resistant watermelon plant introduction pi 595203 (citrullus lanatus var. lanatus) and the zymv-susceptible watermelon cultivar 'new hampshire midget' ('nhm') showed the presence of single nucleotide polymorphisms ...200919820912
establishment of a transgenic hairy root system in wild and domesticated watermelon (citrullus lanatus) for studying root vigor under drought.root vigor is an important trait for the growth of terrestrial plants, especially in water-deficit environments. although deserts plants are known for their highly developed root architecture, the molecular mechanism responsible for this trait has not been determined. here we established an efficient protocol for the genetic manipulation of two varieties of watermelon plants: a desert-grown wild watermelon that shows vigorous root growth under drought, and a domesticated cultivar showing retarda ...201020445980
inoculation of plants with begomoviruses by particle bombardment without cloning: using rolling circle amplification of total dna from infected plants and whiteflies.a new system for inoculation of plants with begomoviral dna without cloning or the use insect vectors is described. total dna extracted from begomovirus-infected plants was amplified by rolling circle amplification (rca) using the bacteriophage phi29 dna polymerase, and inoculated to plants by particle bombardment. infection rates of up to 100% were obtained using this technique. this technique successfully inoculated all the begomoviruses evaluated: five bipartite (bean golden yellow mosaic vir ...201020447420
host range and complete genome sequence of cucurbit chlorotic yellows virus, a new member of the genus crinivirus.cucurbit chlorotic yellows virus (ccyv) causes chlorotic yellows on cucumber (cucumis sativus) and melon (cucumis melo) and is transmitted by bemisia tabaci biotype b and q whiteflies. to characterize the host range of ccyv, 21 cucurbitaceous and 12 other plant species were inoculated using whitefly vectors. all tested cucumis spp. except cucumis anguria and cucumis zeyheri were systemically infected with ccyv, although infection rates varied among species. citrullus lanatus, cucurbita pepo, and ...201020465411
watermelon profilin: characterization of a major allergen as a model for plant-derived food profilins.plant profilins have been reported as minor allergens. they are a well-known pan-allergen family responsible for cross-reactivity between plant-derived foods and pollens. watermelon profilin has been reported to be a major allergen in watermelon (citrullus lanatus).the aim of this study was to characterize recombinant watermelon profilin, confirming its reactivity for diagnostic purposes and the development of immunotherapy.201020484919
genetic diversity in watermelon (citrullus lanatus) landraces from zimbabwe revealed by rapd and ssr markers.low polymorphism in cultivated watermelon has been reported in previous studies, based mainly on us plant introductions and watermelon cultivars, most of which were linked to breeding programmes associated with disease resistance. since germplasm sampled in a putative centre of origin in southern africa may harbour considerably higher variability, dna marker-based diversity was estimated among 81 seedlings from eight accessions of watermelon collected in zimbabwe; five accessions of cow-melons ( ...201020887600
expression in arabidopsis of a nucellus-specific promoter from watermelon (citrullus lanatus).though many tissue-specific promoters have been identified, few have been associated specifically with the angiospermous megasporangium (nucellus). in the present study the 2000-bp regulatory region upstream to the watermelon, citrullus lanatus (thunb.) matsum & nakai, gene wm403 (genbank accession no. af008925), which shows nucellus-specific expression, was cloned from watermelon gdna and fused to the β-glucuronidase reporter gene (gus). the resulting plasmid, wm403 prom::gus(+), which also con ...201021802614
host use of bactrocera latifrons, a new invasive tephritid species in tanzania.bactrocera latifrons (hendel) is a pest of asian origin, first detected in africa in 2006. we assessed the host utilization of this quarantine pest in morogoro region, eastern central tanzania, by collecting a wide range of cultivated and wild host plants of species belonging to solanaceae and cucurbitaceae from april 2007 to april 2008. fruit were collected from 29 plant species and varieties (16 solanaceae and 13 cucurbitaceae) in all agroecological zones of morogoro region. twelve solanaceous ...201020214370
cloning and expression analysis of the ccrboh gene encoding respiratory burst oxidase in citrullus colocynthis and grafting onto citrullus lanatus (watermelon).a full-length drought-responsive gene ccrboh, encoding the respiratory burst oxidase homologue (rboh), was cloned in citrullus colocynthis, a very drought-tolerant cucurbit species. the robh protein, also named nadph oxidase, is conserved in plants and animals, and functions in the production of reactive oxygen species (ros). the ccrboh gene accumulated in a tissue-specific pattern when c. colocynthis was treated with peg, abscisic acid (aba), salicylic acid (sa), jasmonic acid (ja), or nacl, wh ...201020181664
chemical inhibitors of viviparous germination in the fruit of watermelon.it is well known that the seeds of watermelon [citrullus lanatus (thunb.) matsum and nakai] have a high potential to germinate when the fruit has ripened. when removed from the mature fruit, the seeds can germinate under appropriate conditions. however, it is unclear why they cannot germinate in the flesh of the fruit. here, we show that cis-aba and its β-d-glucopyranosyl ester (aba-β-ge) accumulate in the flesh of the fruit at levels high enough to inhibit seed germination. this result indicate ...201020630986
insights into the evolution of mitochondrial genome size from complete sequences of citrullus lanatus and cucurbita pepo (cucurbitaceae).the mitochondrial genomes of seed plants are unusually large and vary in size by at least an order of magnitude. much of this variation occurs within a single family, the cucurbitaceae, whose genomes range from an estimated 390 to 2,900 kb in size. we sequenced the mitochondrial genomes of citrullus lanatus (watermelon: 379,236 nt) and cucurbita pepo (zucchini: 982,833 nt)--the two smallest characterized cucurbit mitochondrial genomes--and determined their rna editing content. the relatively com ...201020118192
sucrose accumulation in watermelon fruits: genetic variation and biochemical analysis.sugar accumulation, the key process determining fruit quality, is controlled by both the translocation of sugars and their metabolism in developing fruits. sugar composition in watermelon, as in all cucurbit fruits, includes sucrose, fructose and glucose. the proportions of these three sugars are determined primarily by three enzyme families: invertases, sucrose synthases (susys) and sucrose phosphate synthases (spss). the goal of the present research was to explore the process of sugar metaboli ...201020036442
purification and partial characterization of low molecular weight vicilin-like glycoprotein from the seeds of citrullus lanatus.the watermelon (citrullus lanatus) seeds are highly nutritive and contain large amount of proteins and many beneficial minerals such as magnesium, calcium, potassium, iron, phosphorous, zinc etc. in various parts of the world, c. lanatus seed extracts are used to cure cancer, cardiovascular diseases, hypertension, and blood pressure. c. lanatus seed extracts are also used as home remedy for edema and urinary tract problems. in this study, we isolated protein fraction of c. lanatus seeds using va ...201121989589
characterization of transcriptome dynamics during watermelon fruit development: sequencing, assembly, annotation and gene expression profiles.cultivated watermelon [citrullus lanatus (thunb.) matsum. & nakai var. lanatus] is an important agriculture crop world-wide. the fruit of watermelon undergoes distinct stages of development with dramatic changes in its size, color, sweetness, texture and aroma. in order to better understand the genetic and molecular basis of these changes and significantly expand the watermelon transcript catalog, we have selected four critical stages of watermelon fruit development and used roche/454 next-gener ...201121936920
anti-inflammatory activities of cucurbitacin e isolated from citrullus lanatus var. citroides: role of reactive nitrogen species and cyclooxygenase enzyme inhibition.the in vivo and in vitro mechanistic anti-inflammatory actions of cucurbitacin e (ce) (citrullus lanatus var. citroides) were examined. the results showed that lps/inf-γ increased no production in raw264.7 macrophages, whereas l-name and ce curtailed it. ce did not reveal any cytotoxicity on raw264.7 and wrl-68 cells. ce inhibited both cox enzymes with more selectivity toward cox-2. intraperitoneal injection of ce significantly suppressed carrageenan-induced rat's paw edema. orac and frap assays ...201121871542
determination of the melon chloroplast and mitochondrial genome sequences reveals that the largest reported mitochondrial genome in plants contains a significant amount of dna having a nuclear origin.the melon belongs to the cucurbitaceae family, whose economic importance among vegetable crops is second only to solanaceae. the melon has a small genome size (454 mb), which makes it suitable for molecular and genetic studies. despite similar nuclear and chloroplast genome sizes, cucurbits show great variation when their mitochondrial genomes are compared. the melon possesses the largest plant mitochondrial genome, as much as eight times larger than that of other cucurbits.201121854637
discovery of second gene for solid dark green versus light green rind pattern in watermelon.the watermelon (citrullus lanatus (thunb.) matsum. & nakai var. lanatus) has high variability for fruit size, shape, rind pattern, and flesh color. this study was designed to measure the qualitative inheritance of rind phenotypes (solid dark green vs. light green). for each of the 2 families, "mountain hoosier" × "minilee" and "early arizona" × "minilee," 6 generations (p(a)s(1), p(b)s(1), f(1), f(2), bc(1)p(a), bc(1)p(b)) were developed. each family was tested in summer 2008 in 3 environments i ...201121566001
dynamic changes in the leaf proteome of a c3 xerophyte, citrullus lanatus (wild watermelon), in response to water deficit.wild watermelon (citrullus lanatus) is a xerophyte native to the kalahari desert, africa. to better understand the molecular mechanisms of drought resistance in this plant, we examined changes in the proteome in response to water deficit. wild watermelon leaves showed decreased transpiration and a concomitant increase in leaf temperature under water deficit conditions. comparison of the proteome of stressed plants with that of unstressed plants by two-dimensional gel electrophoresis revealed tha ...201121259065
muskmelon embryo rescue techniques using in vitro embryo culture.among the major cucurbit vegetables, melon (cucumis melo) has one of the greatest polymorphic fruit types and botanical varieties. some melon fruits have excellent aroma, variety of flesh colors, deeper flavor, and more juice compared to other cucurbits. despite numerous available melon cultivars, some of them are exceedingly susceptible to several diseases. the genetic background carrying the genes for tolerance and/or resistance for those diseases is found in wild melon landraces. unfortunatel ...201121207265
taxonomic significance of trichomes micromorphology in cucurbits.studies on trichomes micromorphology using scanning electron microscope (sem) were undertaken in 23 species with one variety under 13 genera of the family cucurbitaceae (viz., benincasa hispida (thunb.) cogn., citrullus lanatus (thunb.) matsum. & nakai, cucumis melo var. agrestis naudin, cucumis sativus l., diplocyclos palmatus (l.) c. jeffrey, edgaria dargeelingensis c.b. clarke, gynostemma burmanicum king ex chakr., gynostemma pentaphyllum (thunb.) makino, gynostemma pubescens (gagnep.) c.y. w ...201123961108
microplot evaluation of rootstocks for control of meloidogyne incognita on grafted tomato, muskmelon, and watermelon.microplot experiments were conducted over two years (four growing seasons) to evaluate meloidogyne incognita resistance in rootstocks used for grafted tomato (solanum lycopersicum), muskmelon (cucumis melo), and watermelon (citrullus lanatus). three tomato rootstocks; 'tx301', 'multifort', and 'aloha', were tested in addition to the nongrafted scion, 'florida-47'. two muskmelon rootstocks; cucumis metuliferus and 'tetsukabuto' (cucurbita maxima × cucurbita moschata) were evaluated with the nongr ...201123431109
watermelon chlorotic stunt virus (wmcsv): a serious disease threatening watermelon production in jordan.the incidence of watermelon chlorotic stunt disease and the molecular characterization of the jordanian isolate of watermelon chlorotic stunt virus (wmcsv-[jo]) are described in this study. symptomatic leaf samples obtained from watermelon (citrullus lanatus thunb.), melon (cucumis melo l.), squash (cucurbita pepo), cucumber (cucumis sativus l.), and bottle gourd (lagenaria siceraria) plants were tested for wmcsv-[jo] infection by pcr. the virus could be detected in 8 melon and 87 watermelon sam ...201121399920
protective effect of diyarbak-¦r watermelon juice on carbon tetrachloride-induced toxicity in rats.the present study was designed to evaluate the effect of diyarbak-¦r watermelon (citrullus lanatus cv. s++rme) juice on lipid peroxidation states in rat liver, kidney and brain. in vivo administration of carbon tetrachloride (ccl(4)) once a week for 28days caused a significant elevation of serum markers of liver damage, aspartate aminotransferase (ast), alanine aminotransferase (alt), total bilirubin (tb) and decrease in albumin when compared to the control group. however, administration of carb ...201121740951
characterisation and functional properties of watermelon (citrullus lanatus) seed proteins.people in developing countries depend largely on non-conventional protein sources to augment the availability of proteins in their diets. watermelon seed meal is reported to contain an adequate amount of nutritional proteins that could be extracted for use as nutritional ingredients in food products.201120824684
predicting damage of meloidogyne incognita on watermelon.quantitative growth response of watermelon (citrullus lanatus) sensitive to meloidogyne incognita is poorly understood. determination of soil population densities of second-stage juveniles (j2) of m. incognita with baermann funnel extraction often is inaccurate at low soil temperatures. in greenhouse experiments, three sandy soils were inoculated with dilution series of population densities of eggs or j2 of m. incognita and planted in small containers to watermelon 'royal sweet' or subjected to ...201223482631
oil and fatty acid contents in seed of citrullus lanatus schrad.intact seed of 475 genebank accessions of citrullus ( c. lanatus var. lanatus and c. lanatus var. citroides) were analyzed for percent oil content using td-nmr. extracts from whole seed of 96 accessions of c. lanatus (30 var. citroides, 33 var. lanatus, and 33 egusi), c. colocynthis (n = 3), c. ecirrhosus (n = 1), c. rehmii (n = 1), and benincasa fistulosa (n = 3) were also analyzed for their fatty acids content. among the materials analyzed, seed oil content varied from 14.8 to 43.5%. mean seed ...201222540530
comparative mapping in watermelon [citrullus lanatus (thunb.) matsum. et nakai].the first single-nucleotide polymorphism (snp) maps for watermelon [citrullus lanatus (thunb.) matsum. et nakai] were constructed and compared. three populations were developed from crosses between two elite cultivars, klondike black seeded × new hampshire midget (kbs × nhm), an elite cultivar and wild egusi accession, strain ii × pi 560023 (sii × egusi) and an elite cultivar and a wild citron accession, zwrm50 × pi 244019 (zwrm × citroides). the sii × egusi and zwrm × citroides f(2) populations ...201222875176
characterization of the small rna component of leaves and fruits from four different cucurbit species.micrornas (mirnas) are a class of non-coding small rnas involved in post-transcriptional regulation of gene expression critical for plant growth and development, stress responses and other diverse biological processes in plants. the cucurbitaceae or cucurbit family represents some of economically important species, particularly those with edible and medicinal fruits. genomic tools for the molecular analysis of members of this family are just emerging. partial draft genome sequence became availab ...201222823569
proteomic study participating the enhancement of growth and salt tolerance of bottle gourd rootstock-grafted watermelon seedlings.an insertion grafting technique to do research on salt tolerance was applied using watermelon (citrullus lanatus [thunb.] mansf. cv. xiuli) as a scion and bottle gourd (lagenaria siceraria standl. cv. chaofeng kangshengwang) as a rootstock. rootstock-grafting significantly relieved the inhibition of growth and photosynthesis induced by salt stress in watermelon plants. proteomic analysis revealed 40 different expressed proteins in response to rootstock-grafting and/or salt stress. these proteins ...201222771436
lycopene content, antioxidant capacity and colour attributes of selected watermelon (citrullus lanatus (thunb.) mansfeld) cultivars grown in india.the present investigation reports variability in lycopene, ascorbic acid, total phenolics, antioxidant capacity and colour attributes of 12 watermelon cultivars grown in india. antioxidant capacity was evaluated using four in vitro assays, namely ferric reducing antioxidant power, cupric reducing antioxidant capacity, trolox equivalent antioxidant capacity and 2,2-diphenyl-1-picryl hydrazyl. among watermelon cultivars, significant differences (p < 0.05) were found with respect to lycopene conten ...201222716946
cucurbitacin l 2-o-β-glucoside demonstrates apoptogenesis in colon adenocarcinoma cells (ht-29): involvement of reactive oxygen and nitrogen species regulation.emerging evidence suggests that reactive oxygen (ros) and nitrogen (rns) species can contribute to diverse signalling pathways of inflammatory and tumour cells. cucurbitacins are a group of highly oxygenated triterpenes. many plants used in folk medicine to treat cancer have been found to contain cucurbitacins displaying potentially important anti-inflammatory actions. the current study was designed to investigate the anti-ros and -rns effects of cucurbitacin l 2-o-β-glucoside (clg) and the role ...201222685485
medieval emergence of sweet melons, cucumis melo (cucurbitaceae).sweet melons, cucumis melo, are a widely grown and highly prized crop. while melons were familiar in antiquity, they were grown mostly for use of the young fruits, which are similar in appearance and taste to cucumbers, c. sativus. the time and place of emergence of sweet melons is obscure, but they are generally thought to have reached europe from the east near the end of the 15th century. the objective of the present work was to determine where and when truly sweet melons were first developed.201222648880
accumulation of weathered pp'-dde in xylem sap of grafted watermelon.movement of weathered p,p'-dichlorodiphenyldichloroethane (p,p'-dde) from contaminated soil to the rhizosphere pore water to the xylem sap of grafted watermelon was studied under green house conditions. p,p'-dde concentrations in pore water and xylem sap was compared in intact plants, homografted, and compatible heterografts of cucurbita pepo spp. pepo and citrullus lanatus plants. an average p,p'-dde concentrations in pore water of contaminated soil ranged from 0.36 microg/l to 0.55 microg/l an ...201222567720
combining ability analysis in complete diallel cross of watermelon (citrullus lanatus (thunb.) matsum. & nakai).the experiments were carried out in two research stations (mardi bukit tangga, kedah, and mardi seberang perai, penang) in malaysia. the crossings were performed using the four inbred lines in complete diallel cross including selfs and reciprocals. we evaluated the yield components and fruit characters such as fruit yield per plant, vine length, days to fruit maturity, fruit weight, total soluble solid content, and rind thickness over a period of two planting seasons. general combining ability a ...201222566772
a high resolution genetic map anchoring scaffolds of the sequenced watermelon genome.as part of our ongoing efforts to sequence and map the watermelon (citrullus spp.) genome, we have constructed a high density genetic linkage map. the map positioned 234 watermelon genome sequence scaffolds (an average size of 1.41 mb) that cover about 330 mb and account for 93.5% of the 353 mb of the assembled genomic sequences of the elite chinese watermelon line 97103 (citrullus lanatus var. lanatus). the genetic map was constructed using an f(8) population of 103 recombinant inbred lines (ri ...201222247776
accumulation of weathered p,p'-ddts in grafted watermelon.the grafting of melon plants onto cucurbit rootstocks is a common commercial practice in many parts of the world. however, certain cucurbits have been shown to accumulate large quantities of weathered persistent organic pollutants from the soil, and the potential contamination of grafted produce has not been thoroughly evaluated. large pot and field experiments were conducted to assess the effect of grafting on accumulation of weathered ddx (the sum of p,p'-ddt, p,p'-ddd, and p,p'-dde) from soil ...201222224752
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