Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [nutritive value of cooked and uncooked jackfruit seed flour (artocarpus integrifolia) (author's transl)]. | 1974 | 4457964 | |
| lectin(s) extracted from seeds of artocarpus integrifolia (jackfruit): potent and selective stimulator(s) of distinct human t and b cell functions. | a lectin activity that selectively induces different functions of human lymphocytes has been described in a pbs crude extract obtained from the seeds of artocarpus integrifolia (jackfruit). both unfractionated peripheral blood mononuclear cells and purified t cells are strongly stimulated to proliferate by this extract, whereas purified b cells are not. however, the lectin induced a potent polyclonal activation of b cells measured by a reverse hemolytic plaque assay using a multivalent anti-huma ... | 1981 | 6972961 |
| artocarpus integrifolia lectin(s): use and applications in chromosome studies of lymphocyte cultures. | a simple procedure for extracting a lectin from the seeds of the jackfruit, artocarpus integrifolia, is described. the extracts had an average protein concentration of 13.3 mg/ml, and were strongly erythroagglutinating with a 10(6) titre. erythroagglutination was shown to be non-specific for a1-b-0 erythrocytes. lymphoblastic transformation and mitogenic stimulation occurred in lymphocyte cultures both in man and rhesus monkey, and chromosome preparations were obtained. such preparations were of ... | 1982 | 7172755 |
| the preparation of interferon gamma-producing t-cell hybridomas from jacalin-stimulated t lymphocytes and the sh9 t-cell line. | jacalin, a lectin(s) extracted from the seeds of artocarpus integrifolia (jackfruit), was shown to induce the production of gamma interferon (ifn gamma) in human peripheral blood mononuclear cells (pbmc) and human t-lymphocyte cultures. the amount of ifn gamma produced was enhanced in the presence of mezerein, a phorbol ester derivative. fusion of jacalin stimulated t lymphocytes with the sh9 t-cell line resulted in the formation of t-cell hybridomas which spontaneously secreted ifn gamma. the s ... | 1984 | 6094340 |
| jackfruit (artocarpus integrifolia) and the agaricus mushroom lectin fit also to the so-called peanut receptor. | the lectin from jackfruit (artocarpus integrifolia) reacts with the peanut-specific lectin receptor represented by the t-disaccharide, which is also part of the thomsen (= t)-friedenreich antigen, namely o-beta-d-galactosyl-(1----3)-n-acetyl-d-galactosamine. the difference between the jackfruit lectin and peanut lectin has been shown to be mainly due to the further linkage of the t-disaccharide: whereas the peanut lectin reacts with both anomeric forms, jackfruit lectin and the closely - with re ... | 1985 | 3938662 |
| a sperm-agglutinating lectin from seeds of jack fruit (artocarpus heterophyllus). | a lectin specific for n-acetylgalactosamine was isolated from seed extract of jack fruit (artocarpus heterophyllus) by ammonium sulfate precipitation, followed by affinity chromatography on a affigel-galactosamine-agarose column. the lectin possessed agglutinating activities for human and rat sperm as well as human red blood cells. it was found to have mr = 62,000 consisting of two dissimilar subunits of mr = 18,000 and 13,000. it also cross-reacted with an antibody against the lectin of osage o ... | 1985 | 3922368 |
| jacalin: an iga-binding lectin. | we previously reported that seeds of artocarpus integrifolia (jackfruit) contain a lectin, which we call jacalin, that is both a potent t cell mitogen and an apparently t cell-independent activator of human b cells for the secretion of immunoglobulins. during the above experiments we noted a massive precipitation in cell cultures stimulated with greater than or equal to 100 micrograms of lectin. in this paper, we show that the precipitate is formed after the interaction of jacalin and the serum ... | 1985 | 3871459 |
| increase in fluid viscosity during epididymal transit and the immobilization of rat epididymal spermatozoa. | a microcapillary method was developed to measure the viscosity of small volumes of undiluted epididymal fluid. fluid from the cauda epididymis registered 82 +/- 17 centipoise which was much more viscous than fluid from the caput region (8 +/- 2 centipoise). initiation of sperm motility was strongly suppressed in the viscosity range of 7 to 150 centipoise. a significant increase in the viscosity of fluid from the caput region was observed when immobilin in the fluid binds with a lectin from jack ... | 1986 | 3804466 |
| jacalin, a jackfruit lectin, precipitates iga1 but not iga2 subclass on gel diffusion reaction. | precipitation reaction in agarose gel between jacalin, a lectin from jackfruit seeds, and purified immunoglobulins of various classes and their components was studied. single precipitation arcs were observed with monomeric and dimeric iga1 subclass as well as secretory iga1, but not with iga2 of both allotypes, igg, igm, igd, ige, free secretory component or with the j chain. thus, jacalin can be used for differentiating iga1 from iga2 subclass by a simple agarose gel diffusion. | 1986 | 3082992 |
| separation of human siga1 and siga2 by affinity chromatography on the jackfruit lectin, jacalin. | 1987 | 3425425 | |
| the iga-binding lectin jacalin induces complement activation by inhibition of c-1-inactivator function. | jacalin, a d-galactose-specific lectin from jackfruit, interacts with human iga and one or two other serum proteins. incubation of jacalin with fresh human serum was shown to result in activation of the complement system. therefore the mechanism of complement activation by jacalin was studied. jacalin was extracted from jackfruit seeds (crude preparation) and purified to homogeneity by affinity chromatography on iga-sepharose to yield a pure preparation of jacalin. both crude and pure jacalin we ... | 1987 | 3629190 |
| tissue binding patterns of lectins in premalignant and malignant lesions of the oral cavity. | lectins from the seeds of jackfruit (artocarpus integrifolia) and winged bean (psophocarpus tetragonolobus) were isolated using an immobilized n-acetyl d-galactosamine column and conjugated to type vi horse radish peroxidase. the purified conjugate was used for the study of tissue specificities using diaminobenzidine as the substrate on dewaxed tissue sections of normal, oral leukoplakia, oral submucous fibrosis, verucous carcinoma and squamous cell carcinoma of the oral cavity. in spite of havi ... | 1987 | 3449613 |
| a simple procedure for the isolation of human secretory iga of iga1 and iga2 subclass by a jackfruit lectin, jacalin, affinity chromatography. | jackfruit lectin, jacalin, prepared from two batches of jackfruit seeds showed a different specificity in precipitating reaction in agarose gel with various purified immunoglobulins and secretory components. jacalin-p, extracted from jackfruit seeds from the philippines, reacts only with serum iga and secretory iga of iga1 subclass. jacalin-o, extracted from jackfruit seeds from okinawa prefecture in japan, makes a strong precipitin are with iga1 subclass and a weak precipitin arc with iga2 subc ... | 1987 | 3122030 |
| characterization of jacalin, the human iga and igd binding lectin from jackfruit. | the lectin jacalin from the jackfruit artocarpus heterophyllus reacts by precipitation and western blotting with human iga1 and igd but not with iga2 (nor igg and igm). however, it weakly binds iga2 as shown by affinity chromatography and competitive elisa. predominantly reactive carbohydrates are d-galactose and n-acetyl d-galactosamine. jacalin has an apparent mr of about 54,000 and is probably made up of three non-glycosylated and one glycosylated non-covalently linked subunits. its electroph ... | 1987 | 3657793 |
| separation of human iga1 and iga2 using jacalin-agarose chromatography. | a lectin isolated from the tropical jackfruit, jacalin, previously reported to precipitate human immunoglobulin a (iga), and conjugated to agarose was used to separate the two subclasses of iga from secretions. jacalin-agarose binds specifically to the d-galactose moiety of iga1 but not to iga2 which has a different carbohydrate content and structure. iga2 passed through the jacalin-agarose column and was collected in the void volume. iga1 was eluted from the lectin by 0.8 m galactose. of a repr ... | 1987 | 3106500 |
| jacalin, an iga-binding lectin, inhibits differentiation of human b cells by both a direct effect and by activating t-suppressor cells. | jacalin, a lectin extracted from the seeds of artocarpus intergifolia (jackfruit), has been reported to bind specifically to iga while inducing b-cell polyclonal immunoglobulin secretion. we confirmed that jacalin only binds to iga and not to igg or igm and extended these findings by showing that it does not bind to ige. addition of jacalin to either unfractioned peripheral blood lymphocytes or purified b cells failed to induce immunoglobulin synthesis; indeed immunoglobulin production was dimin ... | 1987 | 2948674 |
| a simple procedure for the isolation of human secretory iga of iga1 and iga2 subclass by a jackfruit lectin, jacalin, affinity chromatography. | 1988 | 3374496 | |
| jacalin: isolation, characterization, and influence of various factors on its interaction with human iga1, as assessed by precipitation and latex agglutination. | an iga1-specific lectin, jacalin, was isolated from dried seeds of the jackfruit, artocarpus integrifolia, by affinity binding to iga1-sepharose and elution with d-galactose. jacalin is a glycoprotein with two non-covalently bound subunits (15 and 18 k). interactions between jacalin and human igs were studied by precipitation in gel and in solution, and by agglutination of iga1-coated latex by jacalin. jacalin precipitated only with iga1-containing samples, including monomers, polymers, monoclon ... | 1988 | 3343973 |
| studies on the specificity of the iga-binding lectin, jacalin. | the interactions of iga with the jackfruit lectin, jacalin, were investigated with regard to the specificity of jacalin for species and subclasses of iga. it was found that jacalin selectively bound to human iga1, but not to human iga2, mouse iga or rat iga. binding studies with human iga1 fragments produced by different iga1 proteases revealed that jacalin bound to galactose-terminal oligosaccharides in the hinge region of human iga1. affinity chromatography employing jacalin-sepharose provided ... | 1988 | 3343969 |
| jacalin, the human iga1 and igd precipitating lectin, also binds iga2 of both allotypes. | the lectin jacalin from jackfruit seeds shows a human iga-subclass specificity by gel precipitation and western blotting. however, its reactivity with iga2 is a matter of controversy. we further studied the immunoglobulin isotype specificity of jacalin by affinity chromatography with myeloma sera and by inhibition of jacalin binding to solid-phase iga1 by purified monoclonal immunoglobulins. the lectin proved to bind iga2 of both allotypes with a lower apparent affinity than for iga1 and igd. | 1988 | 3171189 |
| elimination of undesirable immunoglobulin contaminants including aggregated igg from gamma-globulin preparations by jackfruit lectin affinity chromatography. | jackfruit lectin, jacalin, has been characterized to combine with iga of only iga1 subclass but not with iga2 subclass and other immunoglobulins. however, we found in the present study that a lectin from another batch of jackfruit (jacalin-h) showed a different nature in binding reaction. jacalin-h combined with immunoglobulins of every class or subclass except monomer igg. more interestingly, the jacalin-h also combined with aggregated igg. by means of the jacalin-h affinity chromatography, we ... | 1988 | 2454765 |
| autoantibodies to lactate dehydrogenase in serum identified by use of immobilized protein g and immobilized jacalin, a jackfruit lectin. | in this method for identifying autoantibodies to lactate dehydrogenase (anti-lds) in serum, we used immobilized protein g to bind igg-complexed ld and immobilized jacalin to bind iga-complexed ld, leaving non-complexed ld in solution. the non-complexed ld and total ld were kinetically measured. we report results as ld bound to immobilized protein g and ld bound to immobilized jacalin. using sera demonstrating igg and iga anti-lds by immunoelectrophoresis (iep), respectively, we optimized the met ... | 1989 | 2582615 |
| further characterization and immunochemical studies on the carbohydrate specificity of jackfruit (artocarpus integrifolia) lectin. | the lectin from jackfruit (artocarpus integrifolia) seeds has been purified by rivanol (6,9-diamino-2-ethoxyacridine lactate) treatment. the specific activity, molecular weights of parent lectin and its subunit, its glycoprotein nature, and hemagglutination-inhibition assays suggest that this preparation is identical to that obtained by affinity chromatography on melibiose-agarose adsorbent (ahmed, h., and chatterjee, b. p. (1986) in lectins, biology, biochemistry, clinical biochemistry (bøg-han ... | 1989 | 2722839 |
| jacalin: a new laboratory tool in immunochemistry and cellular immunology. | in recent years, a new lectin--jacalin--has raised the interest of immunologists because of its original properties with respect to human immunoglobulins and lymphocytes. its structure and carbohydrate binding specificity are now well documented, and it can be purified easily from jackfruit seeds by ion exchange or affinity chromatography. the binding and precipitating specificities of jacalin with heavy chains of human immunoglobulins allow its use as a diagnostic (iga subclass typing) and prep ... | 1989 | 2668478 |
| jacalin: an excellent lectin for obtaining t cell growth activity from rat spleen cells. | 1. the parameters involved in the choice of an optimal t cell growth activity (tcgac) induction protocol using rat spleen cells stimulated with jacalin were studied. 2. in the absence of serum, 5 micrograms/ml jacalin was sufficient to obtain maximal tcgac. supernatants could be harvested at any time between 24 and 72 h since significant consumption of tcgac was not observed during this interval. tcgac recovery was increased in the presence of 5% fetal calf serum, with the optimal jacalin dose b ... | 1989 | 2636007 |
| a rapid procedure for the purification of iga1 and iga2 subclasses from normal human serum using protein g and jackfruit lectin (jacalin) affinity chromatography. | immunoglobulin a (iga) from pooled normal human sera was purified using antibody and protein g affinity chromatography and gel filtration high-pressure liquid chromatography (hplc). this high purity product was separated into iga1 and iga2 subclasses utilizing the agarose-bound lectin 'jacalin'. evaluation of product homogeneity by immunological testing confirmed greater than 95% purity. the total iga1 and iga2 recovered from sera was approximately 26% of the initial antibody present. | 1989 | 2628285 |
| increased serum levels of iga1-igg immune complexes and anti-f(ab')2 antibodies in patients with primary iga nephropathy. | a solid-phase elisa was used to detect iga1 immune complexes (iga1 ics) containing igg and igm in 38 serum samples from 30 patients with primary iga nephropathy (igan) and 14 subjects with non-iga chronic glomerulonephritis. a jackfruit lectin, jacalin, was used as the substrate for the selective binding of human iga1 ics in serum peg precipitate (7%). the presence of igg, a and m antibodies against the f(ab')2 region of igg was also investigated by the solid-phase elisa. six patients were studi ... | 1989 | 2788538 |
| topography of the combining region of a thomsen-friedenreich-antigen-specific lectin jacalin (artocarpus integrifolia agglutinin). a thermodynamic and circular-dichroism spectroscopic study. | thermodynamic analysis of carbohydrate binding by artocarpus integrifolia (jackfruit) agglutinin (jacalin) shows that, among monosaccharides, me alpha galnac (methyl-alpha-n-acetylgalactosamine) is the strongest binding ligand. despite its strong affinity for me alpha galnac and me alpha gal, the lectin binds very poorly when gal and galnac are in alpha-linkage with other sugars such as in a- and b-blood-group trisaccharides, gal alpha 1-3gal and gal alpha 1-4gal. these binding properties are ex ... | 1990 | 2306217 |
| hypoglycaemic activity of some medicinal plants in sri-lanka. | 1. investigations were carried out to determine whether aqueous extracts of osbeckia octandra, artocarpus heterophyllus and bambusa vulgaris truly possess oral hypoglycaemic activity. 2. all three plant extracts significantly lowered the fasting blood glucose level and markedly improved glucose tolerance in sprague-dawley rats. 3. a maximum hypoglycaemic activity was observed at +3 hr with o. octandra and b. vulgaris; with a. heterophyllus a maximum effect was not observed even at +5 hr. 4. the ... | 1990 | 2276596 |
| structural and functional similarities of breadfruit seed lectin and jacalin. | aquous extracts from seeds of artocarpus altilis (breadfruit) and artocarpus heterophyllus (jackfruit) were compared by polyacrylamide gel electrophoresis. two bands of the same size (12 and 15 kd) as the jacalin subunits were the major components in breadfruit seed extract. they strongly reacted with anti-jacalin antibodies by western blotting. the breadfruit lectin displayed the same igal and igd precipitation specificity as jacalin in gel double diffusion experiments. it also stimulated in vi ... | 1990 | 2111454 |
| jacalin: a lectin mitogenic for human cd4 t lymphocytes. | the major protein component of seeds from jackfruit is the lectin jacalin. jackfruit crude extracts are known to stimulate human lymphocytes, but the mitogenic properties of purified jacalin have not been studied in detail so far. study of the proliferative response of cell populations from normal human peripheral blood to purified jacalin showed it to be mitogenic through an interaction with lymphocytes by its lectin-binding site, as shown by inhibition by iga. jacalin failed to stimulate b cel ... | 1990 | 2372991 |
| iga binding lectins isolated from distinct artocarpus species demonstrate differential specificity. | the discovery of jacalin, a group of lectins from jackfruit seeds (artocarpus heterophyllus), has attracted considerable attention due to its numerous interesting immunological properties as well as its usefulness in the isolation of various serum proteins. we have further identified a similar lectin from the seeds of champedak (artocarpus integer) which we refer to as lectin-c and performed comparative studies with two types of jacalin isolated from different batches of the malaysian jackfruit ... | 1991 | 2062319 |
| effect of artocarpus heterophyllus and asteracanthus longifolia on glucose tolerance in normal human subjects and in maturity-onset diabetic patients. | investigations were carried out to evaluate the effects of hot-water extracts of artocarpus heterophyllus leaves and asteracanthus longifolia whole plant material on the glucose tolerance of normal human subjects and maturity-onset diabetic patients. the extracts of both artocarpus heterophyllus and asteracanthus longifolia significantly improved glucose tolerance in the normal subjects and the diabetic patients when investigated at oral doses equivalent to 20 g/kg of starting material. | 1991 | 2056756 |
| preparation and x-ray characterization of four new crystal forms of jacalin, a lectin from artocarpus integrifolia. | four new crystal forms of the anti-t lectin from jackfruit (artocarpus integrifolia) have been prepared and characterized. three of them, two monoclinic (p21, a = 59.4 a, b = 83.3 a, c = 63.5 a, beta = 107.7 degrees; c2, a = 106.1 a, b = 53.9 a, c = 128.0 a, beta = 95.0 a) and one orthorhombic (c222(1), a = 98.1 a, b = 67.3 a, c = 95.1 a) were grown with 2-methylpentan-2,4-diol (mpd) as the precipitant while the fourth, an hexagonal form (p6(1)22, a = b = 129.6 a, c = 157.9 a), was obtained in t ... | 1991 | 1942030 |
| structural and electron-microscopic studies of jacalin from jackfruit (artocarpus integrifolia) show that this lectin is a 65 kda tetramer. | the 133-amino-acid sequences of the alpha-subunit of jacalin (a lectin from artocarpus integrifolia) and of the slightly larger alpha'-subunit were determined. the alpha'- and alpha-subunits, in the approximate ratio of 1:3, were found to be virtually identical in their primary structures, except for one valine for isoleucine substitution at position 113. although both alpha'- and alpha-chains were glycosylated, the extent of glycosylation in the alpha'-chain was much greater than that in the al ... | 1992 | 1520261 |
| the identification of o-glycosylated precursors of insulin-like growth factor ii. | a procedure that combined ion exchange, gel permeation, and insulin-like growth factor-binding protein 3 (igf-bp-3) affinity chromatography with chromatofocusing and reversed-phase high pressure liquid chromatography was used to isolate high molecular weight precursors of human insulin-like growth factor ii (igf-ii) from acetic acid extracts of cohn fraction iv1. two precursors had isoelectric points (pi) of 5.1 and 5.4 and apparent mr values of 15,000 and 11,500, respectively. an apparent mr = ... | 1992 | 1569071 |
| interaction of artocarpus lectins with human iga does not involve asparagine-linked oligosaccharide of the immunoglobulin. | in view of the controversy with respect to the interaction of jacalin with human iga2, a study was undertaken to assess the reactivity of the artocarpus heterophyllus lectin, as well as the lectin from artocarpus integer (lectin c), with subclasses of human immunoglobulin a by elisa. our data is consistent with the view that artocarpus lectins have no affinity for the iga2 immunoglobulins. in further support of the findings, we have established that n-linked oligosaccharide moieties of iga have ... | 1992 | 1417879 |
| dietary fiber from musa paradisiaca and artocarpus heterophyllus on intestinal mucosal and bacterial beta-glucuronidase activity in hexachlorocyclohexane-treated rats. | 1993 | 7678524 | |
| characterization of glycoproteins from chlamydia trachomatis using lectins. | glycoproteins in chlamydia trachomatis, serotype l1, elementary bodies were studied by lectin blotting. a panel of 23 lectins representing a variety of sugar specificities was used. the pattern of lectin-binding specificities at a peptide band was studied in order to determine the type and structure of its glycoconjugate. to establish chlamydial origin of the glycopeptide bands in the blot, control samples from non-infected host cell membranes were run in parallel. terminal mannosidic structures ... | 1993 | 8286093 |
| identification of a novel 4 kda immunoglobulin-a-binding peptide obtained by the limited proteolysis of jacalin. | jacalin, an iga-binding lectin from jackfruit (artocarpus heterophyllus) seeds, was isolated by the passage of pbs extracts of seeds over an affinity matrix containing iga-sepharose-4b. it was further purified by hplc. when analyzed by sds-page under both reducing and nonreducing conditions, the native jacalin was dissociated into two subunits of 12 and 15.4 kda. both the subunits could bind iga. peptide mapping performed with radioiodinated jacalin indicated that both the subunits were suscepti ... | 1993 | 8431469 |
| comparative analyses of iga1 binding lectins from seeds of six distinct clones of artocarpus integer. | purified lectins from seeds of six distinct clones of artocarpus integer (lectin c) were shown to be structurally and functionally similar. all lectins comprised of two types of non-covalently-linked subunits with apparent m(r) of 13,300 and 16,000. the lectins appeared to interact with several human serum proteins, with the predominance of the iga1 and c1 inhibitor molecules. interaction was not detected with iga2, igd, igg and igm. the lectin cs were also shown to precipitate monkey, sheep, ra ... | 1993 | 8490570 |
| a novel lectin-based enzyme-linked immunosorbent assay for the measurement of iga1 in serum and secretory iga1 in secretions. | jackfruit lectin (jacalin) specifically binds iga1 and secretory iga1 (siga1). jacalin was successfully purified, and used as the immobilized phase in novel noncompetitive enzyme-linked immunosorbent assays (elisas) for the measurement of iga1 in serum and siga1 in saliva, using jacalin affinity purified material as standards. a non-competitive elisa for the measurement of total siga was also developed using commercially available reagents. | 1993 | 8222271 |
| isolation and characterization of cdna clones encoding jacalin isolectins. | four jacalin cdna clones (pskcja1, pskcja3, pskcja15, and pskcja17) have been obtained from an artocarpus integrifolia (jackfruit) seed cdna library. these clones share over 94% sequence homology, and their deduced polypeptide sequences confirm the existence of multiple jacalin isolectins in jackfruit seeds. the deduced amino acid sequences show that jacalin appears to be initially synthesized as a prepropeptide with the following structure: n-signal (21 residues)-->propeptide (39 residues)-->be ... | 1993 | 7680651 |
| the composition and properties of jacalin, a lectin of diverse applications obtained from the jackfruit (artocarpus heterophyllus) seeds. | 1994 | 8070846 | |
| the isolation and characterisation of jacalin [artocarpus heterophyllus (jackfruit) lectin] based on its charge properties. | jackfruit extracts contain a protein termed jacalin which possesses diverse biological properties. a detailed analysis of its charge properties has been lacking. the present investigation was initiated to study isoelectric properties of jacalin in detail and to isolate a single isoform of jacalin. jacalin was isolated from jackfruit extracts by affinity chromatography on immunoglobulin-a immobilised to sepharose 4b. various techniques such as ion-exchange chromatography, isoelectric focusing (ie ... | 1995 | 7767783 |
| maturation of immunoglobulin-a binding proteins in the seeds of jackfruit. | the accumulation of proteins in the jackfruit seeds was monitored by extracting seeds proteins at various stages of growth. the early developing seed had several protein bands distributed over a molecular weight range of 10-67 kda. but the mature seeds contained fewer bands with two of them being prominent at 12 and 15.4 kda. upon isoelectric focusing, the mature seed proteins were resolved into several bands distributed over a ph range of 4-9. while the mature seed proteins contained powerful h ... | 1995 | 7549923 |
| flavones with antibacterial activity against cariogenic bacteria. | methanolic extracts obtained from 13 plants were studied for their antibacterial activity against cariogenic bacteria. among them, the extract from artocarpus heterophyllus showed the most intensive activity. serial chromatographic purifications offered two active compounds which were identified as 6-(3-methyl-1-butenyl)-5,2',4'-trihydroxy-3-isoprenyl-7-methoxy flavone and 5,7,2',4'-tetrahydroxy-6-isoprenylflavone. both isolates completely inhibited the growth of primary cariogenic bacteria at 3 ... | 1996 | 8953432 |
| a novel mode of carbohydrate recognition in jacalin, a moraceae plant lectin with a beta-prism fold. | jacalin, a tetrameric two-chain lectin (66,000 mr) from jackfruit seeds, is highly specific for the tumour associated t-antigenic disaccharide. the crystal structure of jacalin with methyl-alpha-d-galactose reveals that each subunit has a three-fold symmetric beta-prism fold made up of three four-stranded beta-sheets. the lectin exhibits a novel carbohydrate-binding site involving the n terminus of the alpha-chain which is generated through a post-translational modification involving proteolysis ... | 1996 | 8673603 |
| mitogenic activity of new lectins from seeds of wild artocarpus species from vietnam. | proliferative response of human peripheral blood mononuclear cells (pbmc) stimulated by new lectins purified from seeds of differents artocarpus species from vietnam (a. asperulus, a. heterophyllus, a. masticata, a. melinoxylus, a. parva and a. petelotii) was studied and compared to those of the lectin jacalin purified from jackfruit (a. heterophyllus) seeds collected in the island la réunion. all lectins stimulated human pbmc to proliferate, with a variable efficiency of the mitogenic activity. ... | 1996 | 8763740 |
| differential expression of jackfruit-lectin-specific glycoconjugates in metastatic adenocarcinoma and reactive mesothelial cells-a diagnostic aid in effusion cytology. | distinguishing reactive mesothelial cells from adenocarcinoma cells in serous effusions on the basis of morphological criteria alone is often difficult. interest has therefore been focused on identifying reliable methods to supplement the conventional cytological techniques. plant lectins have been reported as diagnostic markers for malignant cells. we studied 51 aspirated samples of benign and malignant effusions using horseradish-peroxidase-conjugated jackfruit lectin. no significant differenc ... | 1996 | 8690755 |
| determination of sugar specificity of jackfruit lectin by a simple sugar-lectin binding assay using microtiter plate. | sugar-lectin binding assay was developed as a simple method which employed direct coating of microtiter plate with galactose-binding lectins. biotin-galactose conjugate was used to bind to the immobilized lectins. the bound conjugate was then detected using streptavidin-horseradish peroxidase. using the assay in conjunction with various competing carbohydrates, jackfruit lectin from artocarpus heterophyllus was found to be specific for alpha-anomer of galactoside with an aromatic residue. | 1997 | 9238539 |
| correlation of lectin binding with lymph node metastasis in oral cancers. | lymph node metastasis is an important factor that influences the treatment policy and prognosis of oral cancers. the cell membrane is known to play a role in the metastatic process. the aim of the present study was to evaluate the relationship of lectin binding frequency of oral cancer cells to their capacity to metastasize to the lymph node. smears collected from tumours of 66 untreated patients were stained with jackfruit lectin (jfl) conjugated with horseradish peroxidase (hrp), with diaminob ... | 1997 | 9192548 |
| oral allergy syndrome to a jackfruit (artocarpus integrifolia). | a 30-year-old man from the philippines with pollen allergy noted the appearance of oral allergy syndrome (oas) after eating raw apple, raw peach, raw celery, and recently, jackfruit (artocarpus integrifolia), a tropical fruit which belongs to the moraceae family (mulberry) and to the genus artocarpus (breadfruit tree). despite the patient's multiple sensitization in skin prick tests and in the pharmacia cap system to birch, grass, mugwort pollen, related fruits and vegetables, and jackfruit, in ... | 1997 | 9188925 |
| homology between jacalin and artocarpin from jackfruit (artocarpus integrifolia) seeds. partial sequence and preliminary crystallographic studies of artocarpin. | jacalin and artocarpin, the two lectins from jackfruit (artocarpus integrifolia) seeds, have different physicochemical properties and carbohydrate-binding specificities. however, comparison of the partial amino-acid sequence of artocarpin with the known sequence of jacalin indicates close to 50% sequence identity. artocarpin crystallizes in two forms, both monoclinic p2(1), with one and two tetramic molecules, respectively, in the asymmetric units of form i (a = 69.9, b = 73.7, c = 60.6 a and be ... | 1997 | 15299916 |
| scavenger and antioxidant properties of prenylflavones isolated from artocarpus heterophyllus. | the antioxidant properties of prenylflavones, isolated from artocarpus heterophyllus lam., was evaluated in this study. among them, artocarpine, artocarpetin, artocarpetin a, and cycloheterophyllin diacetate and peracetate had no effect on iron-induced lipid peroxidation in rat brain homogenate. they also did not scavenge the stable free radical 1,1-diphenyl-2-picrylhydrazyl. in contrast, cycloheterophyllin and artonins a and b inhibited iron-induced lipid peroxidation in rat brain homogenate an ... | 1998 | 9667491 |
| jacalin: a jackfruit (artocarpus heterophyllus) seed-derived lectin of versatile applications in immunobiological research. | jacalin, the major protein from the jackfruit (artocarpus heterophyllus) seeds, is a tetrameric two-chain lectin (molecular mass 65 kda) combining a heavy alpha chain of 133 amino acid residues with a light beta chain of 20-21 amino acid residues. it is highly specific for the alpha-o-glycoside of the disaccharide thomsen-friedenreich antigen (gal beta1-3galnac), even in its sialylated form. this property has made jacalin suitable for studying various o-linked glycoproteins, particularly human i ... | 1998 | 9672207 |
| km+, a mannose-binding lectin from artocarpus integrifolia: amino acid sequence, predicted tertiary structure, carbohydrate recognition, and analysis of the beta-prism fold. | the complete amino acid sequence of the lectin km+ from artocarpus integrifolia (jackfruit), which contains 149 residues/mol, is reported and compared to those of other members of the moraceae family, particularly that of jacalin, also from jackfruit, with which it shares 52% sequence identity. km+ presents an acetyl-blocked n-terminus and is not posttranslationally modified by proteolytic cleavage as is the case for jacalin. rather, it possesses a short, glycine-rich linker that unites the regi ... | 1999 | 10210179 |
| characterisation of jack fruit lectin. | jack fruit (artocarpus heterophyllus) seed extract contains a lectin termed jack fruit lectin (jfl) which possesses diversed biological properties. a detailed analysis of its properties has been lacking. the present investigation was initiated to study the detail properties of jfl. after extraction and purification on affigel galactosamine-agarose column, jfl was subjected to nd-page. several different charged species from nd-page upon sds-page gave rise to two dissimilar trimeric subunit at 12. ... | 2000 | 11192491 |
| jackfruit lectin: properties of mitogenicity and the inhibition of herpesvirus infection. | jackfruit lectin (jfl) from artocarpus heterophyllus has been found to exhibit inhibitory activity in vitro with a cytopathic effect towards herpes simplex virus type 2 (hsv-2), varicella-zoster virus (vzv), and cytomegalovirus (cmv). the 50% inhibitory dose values from plaque reduction assay (inactivation) were 2.5, 5, and 10 eg/ml of jfl for hsv-2, vzv, and cmv, respectively. lymphocyte proliferation was significantly increased in the presence of the jfl in the concentration range of 2.5 to 50 ... | 2000 | 11056557 |
| freeze-substitution of dehydrated plant tissues: artefacts of aqueous fixation revisited. | this investigation assessed the extent of rehydration of dehydrated plant tissues during aqueous fixation in comparison with the fine structure revealed by freeze-substitution. radicles from desiccation-tolerant pea (pisum sativum l.), desiccation-sensitive jackfruit seeds (artocarpus heterophyllus lamk.), and leaves of the resurrection plant eragrostis nindensis ficalho & hiern. were selected for their developmentally diverse characteristics. following freeze-substitution, electron microscopy o ... | 2001 | 11770432 |
| the interaction of selective plant lectins with neuraminidase-treated and untreated iga1 from the sera of iga nephropathy patients. | a study on the binding interaction of lectins from artocarpus heterophyllus (jacalin), glycine max and sambucus nigra with standardised quantity of iga from the iga nephropathy patients and normal controls was performed. the glycine max lectin demonstrated higher affinity towards the serum iga of igan patients as compared to normal controls. however, the affinity binding was lower in cases ofjacalin and the sambucus nigra lectin. when serum samples were treated with neuraminidase, the differenti ... | 2001 | 11419909 |
| artocarpus heterophyllus seeds inhibits sexual competence but not fertility of male rats. | according to ayurvedic literature of sri lanka, roasted seeds of artocarpus heterophyllus lam. (family: moraceae) has aphrodisiac activity. however, some reproductively active young men in rural areas of sri lanka claim that consumption of these seeds few hours prior to coitus disrupts sexual function. because of these two conflicting claims, it was thought useful to scientifically investigate the effects of a. heterophyllus seeds on male sexual function and fertility. this was done using a seed ... | 2002 | 12635700 |
| antibacterial activity of artocarpus heterophyllus. | the crude methanolic extracts of the stem and root barks, stem and root heart-wood, leaves, fruits and seeds of artocarpus heterophyllus and their subsequent partitioning with petrol, dichloromethane, ethyl acetate and butanol gave fractions that exhibited a broad spectrum of antibacterial activity. the butanol fractions of the root bark and fruits were found to be the most active. none of the fractions were active against the fungi tested. | 2003 | 12837372 |
| structural characterization of rubber from jackfruit and euphorbia as a model of natural rubber. | a structural study of low molecular weight rubbers from jackfruit (artocarpus heterophyllus) and painted spurge (euphorbia heterophylla) was carried out as model compounds of natural rubber from hevea brasiliensis. the rubber content of latex from jackfruit was 0.4-0.7%, which is very low compared with that of 30-35% in the latex from hevea tree. the rubber from jackfruit latex was low molecular weight with narrow unimodal molecular weight distribution (mwd), whereas that obtained from e. hetero ... | 2004 | 15360318 |
| carbonised jackfruit peel as an adsorbent for the removal of cd(ii) from aqueous solution. | the fruit of the jack (artocarpus heterophyllus) is one of the popular fruits in india, where the total area under this fruit is about 13,460 ha. a significant amount of peel (approximately 2,714-11,800 kg per tree per year) is discarded as agricultural waste, as apart from its use as a table fruit, it is popular in many culinary preparations. treatment of jackfruit peel with sulphuric acid produced a carbonaceous product which was used to study its efficiency as an adsorbent for the removal of ... | 2004 | 15081486 |
| minerals and functional groups present in the jackfruit seed: a spectroscopic investigation. | in this work, emission and fourier transform infrared spectra of the jackfruit seed, in powdered form, are recorded. analysis of the emission spectrum confirms the presence of two hitherto undetected elements, manganese and magnesium. the fourier transform infrared spectrum reveals the presence of some specific functional groups, attributed to the different bands present in the spectrum. | 2004 | 15762312 |
| allergy to jackfruit: a novel example of bet v 1-related food allergy. | jackfruit allergy has been reported just once. it is unknown whether this food allergy is caused by direct sensitization or cross-sensitization to pollen allergens. | 2004 | 15461600 |
| structural basis for the carbohydrate specificities of artocarpin: variation in the length of a loop as a strategy for generating ligand specificity. | artocarpin, a tetrameric lectin of molecular mass 65 kda, is one of the two lectins extracted from the seeds of jackfruit. the structures of the complexes of artocarpin with mannotriose and mannopentose reported here, together with the structures of artocarpin and its complex with me-alpha-mannose reported earlier, show that the lectin possesses a deep-seated binding site formed by three loops. the binding site can be considered as composed of two subsites; the primary site and the secondary sit ... | 2004 | 15099743 |
| a basis for estimation of consumption: literature values for selected food volatiles. part iii. | quantitative data on volatile compounds have been reported in 16 food items. no publications reporting quantitative data were found for two of these 16 food products, i.e. avocado and jackfruit. about 550 volatile compounds have been assayed globally in the other 14 food products. mango and raspberry were the products with the greatest number of volatile compounds; the most representative substances were benzaldehyde, ethyl acetate, limonene, and 2-phenylethanol. | 2004 | 2647533 |
| antiinflammatory flavonoids from artocarpus heterophyllus and artocarpus communis. | the antiinflammatory activities of the isolated flavonoids, including cycloartomunin (1), cyclomorusin (2), dihydrocycloartomunin (3), dihydroisocycloartomunin (4), cudraflavone a (5), cyclocommunin (6), and artomunoxanthone (7), and cycloheterohyllin (8), artonins a (9) and b (10), artocarpanone (11), artocarpanone a (12), and heteroflavanones a (13), b (14), and c (15) from artocarpus communis and a. heterophyllus, were assessed in vitro by determining their inhibitory effects on the chemical ... | 2005 | 15884809 |
| structural basis for the energetics of jacalin-sugar interactions: promiscuity versus specificity. | jacalin, a tetrameric lectin, is one of the two lectins present in jackfruit (artocarpus integrifolia) seeds. its crystal structure revealed, for the first time, the occurrence of the beta-prism i fold in lectins. the structure led to the elucidation of the crucial role of a new n terminus generated by post-translational proteolysis for the lectin's specificity for galactose. subsequent x-ray studies on other carbohydrate complexes showed that the extended binding site of jacalin consisted of, i ... | 2005 | 15733927 |
| structural characterization of novel chitin-binding lectins from the genus artocarpus and their antifungal activity. | two novel chitin-binding lectins from seeds of artocarpus genus were described in this paper, one from a. integrifolia (jackfruit) and one from a. incisa (breadfruit). they were purified from saline crude extract of seeds using affinity chromatography on chitin column, size-exclusion chromatography and reverse-phase chromatography on the c-18 column. both are 14 kda proteins, made up of 3 chains linked by disulfide bonds. the partial amino acid sequences of the two lectins showed they are homolo ... | 2006 | 16257591 |
| screening of traditional antidiabetic medicinal plants of mauritius for possible alpha-amylase inhibitory effects in vitro. | in this study, seven exotic/indigenous medicinal plants of mauritius, namely coix lacryma-jobi (poaceae), aegle marmelos (rutaceae), artocarpus heterophyllus (moraceae), vangueria madagascariensis (rubiaceae), azadirachta indica (meliaceae), eriobotrya japonica (rosaceae) and syzigium cumini (myrtaceae) were studied for possible effects on starch breakdown by alpha-amylase in vitro. the results showed that only artocarpus heterophyllus significantly (p < 0.05) inhibited alpha-amylase activity in ... | 2006 | 16521114 |
| elucidation of binding specificity of jacalin toward o-glycosylated peptides: quantitative analysis by frontal affinity chromatography. | jacalin, a lectin from the jackfruit artocarpus integrifolia, has been known as a valuable tool for specific capturing of o-glycoproteins such as mucins and iga1. though its sugar-binding preference for t/tn-antigens is well established, its detailed specificity has not been elucidated. in this study, we prepared a series of mucin-type glycopeptides using human glycosyltransferases, that is, st6galnac1, core1gal-t1 and -t2, beta3gn-t6, and core2gnt1, and investigated their binding to immobilized ... | 2006 | 16177266 |
| inhibitory effect of artocarpanone from artocarpus heterophyllus on melanin biosynthesis. | in our previous efforts to find new tyrosinase inhibitory materials, we investigated 44 indonesian medicinal plants belonging to 24 families. among those plants, the extract of artocarpus heterophyllus was one of the strongest inhibitors of tyrosinase activity. by activity-guided fractionation of a. heterophyllus wood extract, we isolated artocarpanone, which inhibited both mushroom tyrosinase activity and melanin production in b16 melanoma cells. this compound is a strong candidate as a remedy ... | 2006 | 16946519 |
| inhibitory effect of isoprenoid-substituted flavonoids isolated from artocarpus heterophyllus on melanin biosynthesis. | isoprenoid-substituted flavonoids were isolated from the wood of artocarpus heterophyllus by means of activity-guided fractionation. artocarpin (1), cudraflavone c (2), 6-prenylapigenin (3), kuwanon c (4), norartocarpin (5) and albanin a (6) inhibited melanin biosynthesis in b16 melanoma cells without inhibiting tyrosinase. a structure-activity investigation indicated that the presence of the isoprenoid-substituted moiety enhanced the inhibitory activity on melanin production in b16 melanoma cel ... | 2006 | 16732541 |
| comparisons of low-cost adsorbents for treating wastewaters laden with heavy metals. | in this article, the removal performance and cost-effectiveness of various low-cost adsorbents derived from agricultural waste, industrial by-product or natural material are evaluated and compared to those of activated carbon for the removal of heavy metals (cd(ii), cr(iii), cr(vi), cu(ii), ni(ii) and zn(ii)) from metals-contaminated wastewater. to highlight their technical applicability, selected information on ph, dose required, initial metal concentration, adsorption capacity and the price of ... | 2006 | 16300818 |
| structure-activity relationship of prenyl-substituted polyphenols from artocarpus heterophyllus as inhibitors of melanin biosynthesis in cultured melanoma cells. | a series of prenylated, flavone-based polyphenols, compounds 1-8, were isolated from the wood of artocarpus heterophyllus. these compounds, which have previously been shown not to inhibit tyrosinase activity, were found to be active inhibitors of the in vivo melanin biosynthesis in b16 melanoma cells, with little or no cytotoxicity. to clarify the structural requirement for inhibition, some structure-activity relationships were studied, in comparison with related compounds lacking prenyl side ch ... | 2007 | 17886834 |
| solid-state fermentation for the production of monascus pigments from jackfruit seed. | the aim of the present work was to investigate the feasibility of jackfruit seed powder as a substrate for the production of pigments by monascus purpureus in solid-state fermentation (ssf). a pigment yield of 25odunits/g dry fermented substrate was achieved by employing jackfruit seed powder with optimized process parameters such as 50% initial moisture content, incubation temperature 30 degrees c, 9x10(4)spores/g dry substrate inoculum and an incubation period of seven days. the color of the p ... | 2007 | 16919934 |
| glycosylation-dependent interaction of jacalin with cd45 induces t lymphocyte activation and th1/th2 cytokine secretion. | jacalin, an alpha-o-glycoside of the disaccharide thomsen-friedenreich antigen (galactose beta1-3 n-acetylgalactosamine, t-antigen)-specific lectin from jackfruit seeds, has been shown to induce mitogenic responses and to block infection by hiv-1 in cd4+ t lymphocytes. the molecular mechanism underlying jacalin-induced t cell activation has not been elucidated completely yet. in the present study, protein tyrosine phosphatase (ptpase) cd45 was isolated from a jurkat t cell membrane fraction as a ... | 2007 | 17242371 |
| antioxidant activities of some local bangladeshi fruits (artocarpus heterophyllus, annona squamosa, terminalia bellirica, syzygium samarangense, averrhoa carambola and olea europa). | in the present study, antioxidant activities of the fruits of a. heterophyllus, a. squamosa, t. bellirica, s. samarangense, a. carambola and o. europa were investigated. for this, at first matured fruits of them were sliced into small pieces and dried in the sun and finally crushed in a grinder to make powder. ethanolic extracts of fruit powder were prepared using 99.99% ethanol. the antioxidative activities of these extracts were determined according to their abilities of scavenging 1, 1-diphen ... | 2007 | 17460898 |
| two new ascomycetous anamorphic yeast species related to candida friedrichii--candida jaroonii sp. nov., and candida songkhlaensis sp. nov.--isolated in thailand. | in a study of yeast diversity in thailand, eight strains of hitherto undescribed anamorphic yeasts were isolated: four from insect frass, two from marasmius sp. fruiting bodies, one from a flower, and one from jackfruit exudates. phylogenetic analysis of the d1/d2 domain of 26s ribosomal dna nucleotide sequences indicated that the eight strains represented two new species related to candida friedrichii. genetic separation of the two new species was further supported by dna-dna hybridization anal ... | 2008 | 18425597 |
| evaluation of genetic diversity in jackfruit (artocarpus heterophyllus lam.) based on amplified fragment length polymorphism markers. | artocarpus heterophyllus lam., commonly called jackfruit, is a medium-sized evergreen tree that bears high yields of the largest known edible fruit. yet, it has been little explored commercially due to wide variation in fruit quality. the genetic diversity and genetic relatedness of 50 jackfruit accessions were studied using amplified fragment length polymorphism markers. of 16 primer pairs evaluated, eight were selected for screening of genotypes based on the number and quality of polymorphic f ... | 2008 | 18752192 |
| therapeutic administration of km+ lectin protects mice against paracoccidioides brasiliensis infection via interleukin-12 production in a toll-like receptor 2-dependent mechanism. | km(+) is a mannose-binding lectin from artocarpus integrifolia that induces interleukin (il)-12 production by macrophages and protective t helper 1 immune response against leishmania major infection. in this study, we performed experiments to evaluate the therapeutic activity of jackfruit km(+) (jfkm(+)) and its recombinant counterpart (rkm(+)) in experimental paracoccidioidomycosis. to this end, jfkm(+) or rkm(+) was administered to balb/c mice 10 days after infection with paracoccidiodes brasi ... | 2008 | 18599609 |
| analysis of the sugar-binding specificity of mannose-binding-type jacalin-related lectins by frontal affinity chromatography--an approach to functional classification. | the jacalin-related lectin (jrl) family comprises galactose-binding-type (gjrls) and mannose-binding-type (mjrls) lectins. although the documented occurrence of gjrls is confined to the family moraceae, mjrls are widespread in the plant kingdom. a detailed comparison of sugar-binding specificity was made by frontal affinity chromatography to corroborate the structure-function relationships of the extended mjrl subfamily. eight mjrls covering a broad taxonomic range were used: artocarpin from art ... | 2008 | 18266762 |
| comparative study of the chemical composition and mineral element content of artocarpus heterophyllus and treculia africana seeds and seed oils. | a comparative study of artocarpus heterophyllus and treculia africana seeds, both of moraceae family, was carried out to establish their chemical compositions and evaluate their mineral element content in order to investigate the possibility of using them for human and or animal consumption and also to examine if there is a relationship between the properties of these seeds. a. heterophyllus and t. africana are rich in protein; their protein contents are higher than those from high protein anima ... | 2008 | 17959379 |
| isolation of tyrosinase inhibitors from artocarpus heterophyllus and use of its extract as antibrowning agent. | a new furanoflavone, 7-(2,4-dihydroxyphenyl)-4-hydroxy-2-(2-hydroxy propan-2-yl)-2, 3-dihydrofuro(3, 2-g)chromen-5-one (artocarpfuranol, 1), together with 14 known compounds, dihydromorin (2), steppogenin (3), norartocarpetin (4), artocarpanone (5), artocarpesin (6), artocarpin (7), cycloartocarpin (8), cycloartocarpesin (9), artocarpetin (10), brosimone i (11), cudraflavone b (12), carpachromene (13), isoartocarpesin (14), and cyanomaclurin (15) were isolated from the wood of artocarpus heterop ... | 2008 | 18683821 |
| anti-inflammatory effects of phenolic compounds isolated from the fruits of artocarpus heterophyllus. | artocarpus heterophyllus lam is a large evergreen tree cultivated throughout southeast asia for its fruits. its leaves and roots have been used for medicinal purposes. the aim of this work was to study the in vitro anti-inflammatory effects of phenolic compounds isolated from the ethyl acetate extracts of the fruits of artocarpus heterophyllus. three phenolic compounds were characterized as artocarpesin [5,7,2',4'-tetrahydroxy-6-(3-methylbut-3-enyl) flavone] ( 1), norartocarpetin (5,7,2',4'-tetr ... | 2008 | 18500810 |
| specialized use of two fingers in free-ranging aye-ayes (daubentonia madagascariensis). | the aye-aye (daubentonia madagascariensis) possesses a highly specialized hand with two fingers, the third and the fourth, being used in a way unparalleled by any other primate. we observed the use of the third and the fourth fingers in various activities in four free-ranging aye-ayes. we found that the thin third finger was used exclusively or preferably for tapping, inserting into the mouth (probably for cleaning the teeth) and probing for nectar, kernels and insects in bamboo, twigs and live ... | 2008 | 18473358 |
| removal of cationic dye from aqueous solution using jackfruit peel as non-conventional low-cost adsorbent. | this study aimed at investigating the feasibility of using jackfruit peel (jfp), a solid waste, abundantly available in malaysia, for the adsorption of methylene blue, a cationic dye. batch adsorption studies were conducted to evaluate the effects of contact time, initial concentration (35-400mg/l), ph (2-11), and adsorbent dose (0.05-1.20g) on the removal of dye at temperature of 30 degrees c. the experimental data were analyzed by the four different types of linearized langmuir isotherm, the f ... | 2009 | 18572309 |
| chemical components and tyrosinase inhibitors from the twigs of artocarpus heterophyllus. | an hplc method was developed and validated to compare the chemical profiles and tyrosinase inhibitors in the woods, twigs, roots, and leaves of artocarpus heterophyllus . five active tyrosinase inhibitors including dihydromorin, steppogenin, norartocarpetin, artocarpanone, and artocarpesin were used as marker compounds in this hplc method. it was discovered that the chemical profiles of a. heterophyllus twigs and woods are quite different. systematic chromatographic methods were further applied ... | 2009 | 19588925 |
| adsorption of methylene blue from aqueous solution by jackfruit (artocarpus heteropyllus) leaf powder: a fixed-bed column study. | continuous fixed-bed studies were undertaken to evaluate the efficiency of jackfruit leaf powder (jlp) as an adsorbent for the removal of methylene blue (mb) from aqueous solution under the effect of various process parameters like bed depth (5-10cm), flow rate (30-50ml/min) and initial mb concentrations (100-300mg/l). the ph at point of zero charge (ph(pzc)) of the adsorbent was determined by the titration method and a value of 3.9 was obtained. a ftir of the adsorbent was done before and after ... | 2009 | 19541403 |
| carotenoid composition of jackfruit (artocarpus heterophyllus), determined by hplc-pda-ms/ms. | carotenoids are pigments responsible for the yellow-reddish color of many foods and are related to important functions and physiological actions, preventing several chronic-degenerative diseases. the objective of this study was to confirm the carotenoid composition of jackfruit by high-performance liquid chromatography connected to photodiode array and mass spectrometry detectors (hplc-pda-ms/ms). the main carotenoids were all-trans-lutein (24-44%), all-trans-beta-carotene (24-30%), all-trans-ne ... | 2009 | 19437120 |
| wound healing properties of artocarpus heterophyllus lam. | the studies on excision wound healing model reveals significant wound healing activity of the methanolic leaf extract (simple ointment 5%) of "artocarpus heterophyllus" ham which is comparable with standard (betadine). in the excision model, the period of epithelization, of the extract treated group was found to be higher than the controlgroup and slightly lesser than standard treated group of animals on the up to 16(th) post wounding day. | 2009 | 22557331 |
| a simple micro-method for determining precise oligosaccharidic specificity of mannose-binding lectins. | a simple and inexpensive method was developed to rapidly define the specificity of mannose-specific lectins toward oligomannoside-type structures. the method involved the interaction of a mixture of n-[(14)c]-acetylated glycoasparagines, prepared by exhaustive pronase digestion of bovine pancreatic ribonuclease b and n-[(14)c]-acetylation with [(14)c]-acetic anhydride and containing all the possible oligomannoside-type n-glycans, with the lectin immobilized on sepharose-4b. after exhaustive desa ... | 2009 | 19542523 |
| evaluation of agrowastes as immobilizers for probiotics in soy milk. | the objective of this study was to evaluate agricultural wastes as immobilizers for probiotics in liquid foods, such as soy milk. probiotic strains were initially evaluated for acid and bile tolerance and the ability to produce alpha-galactosidase. rinds of durian, mangosteen, and jackfruit were dried, ground, and sterilized prior to immobilization of selected strains ( lactobacillus acidophilus ftdc 1331, l. acidophilus ftdc 2631, l. acidophilus ftdc 2333, l. acidophilus ftdc 1733, and lactobac ... | 2009 | 19821558 |
| the lectin jacalin induces human b-lymphocyte apoptosis through glycosylation-dependent interaction with cd45. | it has been well established that cd45 is a key receptor-type protein tyrosine phosphatase (ptpase) regulating src-family protein tyrosine kinase (src-ptk) in t and b lymphocytes. however, precisely how cd45 exerts its effect in these lymphocytes remains controversial. we recently reported that jacalin, an alpha-o-glycoside of the disaccharide thomsen-friedenreich antigen-specific lectin from jackfruit seeds, caused marked t-cell activation in response to t-cell receptor ligation and cd28 costim ... | 2009 | 19175793 |
| simian immunodeficiency virus from the sooty mangabey and rhesus macaque is modified with o-linked carbohydrate. | although stretches of serine and threonine are sometimes sites for o-linked carbohydrate attachment, specific sequence and structural determinants for o-linked attachment remain ill defined. the gp120 envelope protein of sivmac239 contains a serine-threonine-rich stretch of amino acids at positions 128 to 139. here we show that lectin protein from jackfruit seed (jacalin), which binds to non- and monosialylated core 1 o-linked carbohydrate, potently inhibited the replication of sivmac239. select ... | 2010 | 20962077 |
| antioxidant and antibacterial activities on foodborne pathogens of artocarpus heterophyllus lam. (moraceae) leaves extracts. | total water extract, ethyl acetate, and aqueous fractions from the leaves of artocarpus heterophyllus were evaluated for phenolic content, antioxidant, and antibacterial activities against some foodborne pathogens such as e. coli, listeria monocytogenes, salmonella typhimurium, salmonella enterica, bacillus cereus, enterococcus faecalis, and staphylococcus aureus. the minimum inhibitory concentration (mics) of extract and fractions determined by the agar dilution method were ranged from 221.9 mi ... | 2010 | 20629886 |
| a statistical approach for optimization of polyhydroxybutyrate production by bacillus sphaericus ncim 5149 under submerged fermentation using central composite design. | the aim of this work was to statistically optimize the cultural and nutritional parameters for the production of polyhydroxybutyrate (phb) under submerged fermentation using jackfruit seed hydrolysate as the sole carbon source. on the basis of results obtained from "one variable at a time" experiment, inoculum age, jackfruit seed hydrolysate concentration, and ph were selected for response surface methodology studies. a central composite design (ccd) was employed to get the optimum level of thes ... | 2010 | 19812909 |