Publications

[interrelationship and classification of micro-organisms based on the adansonian taxonomic theory of "similarity value" and the "center species" theory. (3). tribe neisserieae of the family coccaceae and a new genus, halococcus theoretically isolated and verified].		1966	6009585
[adansonian taxonomy and the relationship of microorganisms based on the concepts of similarity value and center species. 5. biological characteristics of the new genus halococcus with special reference to the correlation between experimental findings and theoretically deducted features].		1968	5749850
characterization of the deoxyribonucleic acid of various strains of halophilic bacteria.	bacteria classified as extreme halophiles, in the genera halobacterium and halococcus, contain deoxyribonucleic acid (dna) which displays two components in a cscl equilibrium density gradient. the base composition of the major dna component ranges from 66 to 68% guanine plus cytosine (gc), whereas that of the satellite dna comprising some 11 to 36% of the total, is between 57 and 60% gc. purification of the bacterial cells in a cscl density gradient and other more conventional strain purificatio ...	1969	4979441
2-amino-2-deoxyguluronic acid: a constituent of the cell wall of halococcus sp., strain 24.		1974	4426055
microbiological study of a hypersaline lake in french somaliland.	in a study of a lake having a higher concentration of salts than the dead sea, all of the heterotrophic bacteria isolated were aerobes; no strictly anaerobic strains were found. ninety percent of the strains were euryhalines and ten percent were strict halophiles. the extreme halophiles belonged to the species halobacterium trapanicum and halococcus morrhuae.	1974	4833284
halococcus morrhuae: a sulfated heteropolysaccharide as the structural component of the bacterial cell wall.	the qualitative and quantitative composition of purifed cell wall of halococcus morrhuae ccm 859 was determined. glucose, mannose, galactose; glucuronic and galacturonic acids; glucosamine, galactosamine, gulosaminuronic acid; acetate, glycine and sulfate are found as major constituents. the amino sugars are n-acetylated. it was not possible to fractionate the cell wall in chemically different polymers. evidence is presented that the major cell wall polymer of this strain is a complex heterolgyc ...	1975	1200739
potash guar gum--a source of halophilic bacteria.	moderate and extreme halophilic microorganisms have been isolated from guar gum solutions dissolved in potash brine for use in sylvite froth flotation. tentative identifications have been made of vibrio, halobacterium, and halococcus species. reinoculation of these isolates into guar gum solutions has shown that they are not carbohydrate-utilizing strains, but instead metabolize the contaminating proteins present in guar.	1976	974914
lack of peptidoglycan in the cell walls of methanosarcina barkeri.	neither muramic acid and glucosamine nor d-glutamic acid or other amino acids typical of peptidoglycan were found in cell walls of two strains of methanosarcina barkeri. the main components are galactosamine, neutral sugars and uronic acids. therefore, the structural component of the cell wall most likely consists of an acid heteropolysaccharide, resembling that of halococcus morrhuae. it is, however, not sulfated.	1977	889387
isolation of extreme halophiles from seawater.	extreme halophilic bacteria were isolated from the ocean off the coast of spain. all were gram-negative cocci. one isolate was compared to halococcus sp. ncmb 757 and was found to have similar characteristics.	1979	16345409
structure of a modified nucleoside in archaebacterial trna which replaces ribosylthymine. 1-methylpseudouridine.	the structure of a modified nucleoside isolated from halococcus morrhuae trna has been established as 1-methylpseudouridine, based on mass spectrometry and gas chromatography carried out on 0.2 a260 unit of material. not previously found in nucleic acids, 1-methylpseudouridine appears to be unique to archaebacterial trna, where it replaces ribosylthymine in the t psi c loop of most of the trnas examined thus far.	1982	7061499
dna-dependent rna polymerase from the extremely halophilic archaebacterium halococcus morrhuae.	pure and absolutely dna-dependent rna polymerase has been isolated from the extremely halophilic archaebacterium, halococcus morrhuae. it is composed of five heavy (142 000; 88 000; 73 000; 52 500; and 49 500 da) and five small components (13 300; 11 200; 10 800; 10 500; 9 900 da). the peptides of 49 500 da and 52 500 da probably represent one component in different modification states. single-stranded dna shows the highest template efficiency, although archaebacterial chromosomal dnas are effic ...	1983	6884365
growth potential of halophilic bacteria isolated from solar salt environments: carbon sources and salt requirements.	eighteen strains of extremely halophilic bacteria and three strains of moderately halophilic bacteria were isolated from four different solar salt environments. growth tests on carbohydrates, low-molecular-weight carboxylic acids, and complex medium demonstrated that the moderate halophiles and strains of the extreme halophiles haloarcula and halococcus grew on most of the substrates tested. among the halobacterium isolates were several metabolic groups: strains that grew on a broad range of sub ...	1984	16346609
the nucleotide sequence of the 16s ribosomal rna gene of the archaebacterium halococcus morrhua.	the sequence of the 16s rrna gene from the archaebacterium halococcus morrhua was determined by the dideoxynucleotide sequencing method. it is 1475 nucleotides long. this is the second archaebacterial sequence to be determined and it provides sequence comparison evidence for the secondary structural elements confined to the rnas of this kingdom and, also, support for controversial or additional base pairing in the eubacterial rnas. six structural features are localized that have varied during th ...	1984	6430699
[absorption of 14c-dicarboxylic acids by bacteria of the family halobacteriaceae].	cultures of the family halobacteriaceae belonging to the species halobacterium halobium, h. cutirubrum, h. vallismortis and halococcus morrhuae were shown to be capable of assimilating 14c-succinate. halobacterium salinarium lacked this ability. the transport systems of c4-dicarboxylates differed in halobacterium halobium 996 and h. vallismortis 1398, on the one hand, and halococcus morrhuae 1235, on the other. the differences involve the kinetic parameters and stereospecificity of transport sys ...	1984	6748971
[comparative characteristics of the transport systems of c4-dicarboxylic acids in cultures of the genera halobacterium and halococcus].	the transport systems of the extreme halophilic organisms, halobacterium and halococcus, differ to a considerable degree in the kinetic parameters of succinate and fumarate transport, the exchange between exocellular and endocellular labeled dicarboxylates, and the rate at which labeled compounds in the cell are incorporated into cellular metabolism.	1984	6748976
the nucleotide sequence of the gene coding for the 16s rrna from the archaebacterium halobacterium halobium.	the complete 1473-bp sequence of the 16s rrna gene from the archaebacterium halobacterium halobium has been determined. alignment with the sequences of the 16s rrna gene from the archaebacteria halobacterium volcanii and halococcus morrhua reveals similar degrees of homology, about 88%. differences in the primary structures of h. halobium and eubacterial (escherichia coli) 16s rrna or eukaryotic (dictyostelium discoideum) 18s rrna are much higher, corresponding to 63% and 56% homology, respectiv ...	1985	2414154
polyamines in photosynthetic eubacteria and extreme-halophilic archaebacteria.	qualitative and quantitative determinations of polyamines have been done in 4 photosynthetic eubacteria and 6 extreme-halophilic archaebacteria. for comparison, 5 moderate-halophilic eubacteria were also analyzed to determine their polyamine contents. not only putrescine and spermidine but also homospermidine were found in the photosynthetic eubacteria, especially in the n2-fixing species, rhodospirillum and chromatium. norspermidine, norspermine, and spermine were not detected in the phototroph ...	1985	3928615
ish51: a large, degenerate family of insertion sequence-like elements in the genome of the archaebacterium, halobacterium volcanii.	we describe a new family of repetitive elements in the genome of the archaebacterium halobacterium volcanii. there are some 20-30 copies of this element, which we designate ish51. sequenced copies show typical insertion sequence characteristics (terminal inverted repeats, direct flanking repeats of "target site" dna). however, members of the ish51 family are highly heterogeneous, showing on average only 85% primary sequence homology; and some genomic copies appear to be severely truncated. some ...	1986	3020501
evolutionary relationships amongst archaebacteria. a comparative study of 23 s ribosomal rnas of a sulphur-dependent extreme thermophile, an extreme halophile and a thermophilic methanogen.	the 23 s rna genes representative of each of the main archaebacterial subkingdoms, desulfurococcus mobilis an extreme thermophile, halococcus morrhuae an extreme halophile and methanobacterium thermoautotrophicum a thermophilic methanogen, were cloned and sequenced. the inferred rna sequences were aligned with all the available 23 s-like rnas of other archaebacteria, eubacteria/chloroplasts and the cytoplasm of eukaryotes. universal secondary structural models containing six major structural dom ...	1987	3116261
[nucleotide composition and homologies in the dna of new extreme halophilic soil archaebacteria].	dna nucleotide composition was studied in extreme halophilic bacteria belonging to the genera halobacterium, halococcus, natronobacterium and natronococcus. the cultures were shown to be a monolithic group of microorganisms with the content of gc pairs typical of extreme halophilic archebacteria. the difference between the content of dna major and minor components was twice as high in halobacterium distributus strains isolated from sulfate saline soils as compared to cultures of this species iso ...	1987	3449743
distribution and types of bacteria isolated from an inland saltern.	the physico-chemical characteristics and bacterial populations of an inland saltern were studied. the well water leading into the ponds of the saltern differed from sea water in its total salinity (18% (w/v)) and had lower mg2+ and so4(2-) contents and much higher contents of cl-, ca2+- and k+. a high proportion of halotolerant microorganisms were isolated, although marine bacteria were not found. most of the halophilic strains isolated appeared to be typical moderate halophiles, and included si ...	1987	3606874
lysis of halobacteria in bacto-peptone by bile acids.	all tested strains of halophilic archaebacteria of the genera halobacterium, haloarcula, haloferax, and natronobacterium lysed in 1% bacto-peptone (difco) containing 25% nacl, whereas no lysis was observed with other strains belonging to archaebacteria of the genera halococcus, natronococcus, and sulfolobus, methanogenic bacteria, and moderately halophilic eubacteria. substances in bacto-peptone which caused lysis of halobacteria were purified and identified as taurocholic acid and glycocholic a ...	1988	16347619
sequence, organization, transcription and evolution of rna polymerase subunit genes from the archaebacterial extreme halophiles halobacterium halobium and halococcus morrhuae.	the genes for the four largest subunits, a, b', b" and c, of the dna-dependent rna polymerase were cloned from the extreme halophile halobacterium halobium and sequenced and their transcription was analyzed. the downstream half of this gene cluster from another extreme halophile halococcus morrhuae was also cloned, sequenced and its transcription products characterized. the h. halobium genes were transcribed into a common transcript from an upstream promoter in the order b", b', a and c. they ar ...	1989	2495365
polar lipids of non-alkaliphilic halococci.	until recently, only one species of halococcus has been recognized, namely, h. morrhuae, but a large number of extremely halophilic non-alkaliphilic cocci have now been isolated from hypersaline habitats in spain and classified into four phenons (a-d); one of the phenon d strains has been classified as a new species, halococcus saccharolyticus. examination of the lipids of h. saccharolyticus and four strains of phenons a-c showed the presence in all of them of c20-c20 and c20-c25 diether molecul ...	1990	2223853
nucleotide sequences of 16s rrna encoding genes from halophilic archaea halococcus morrhuae nrc16008 and haloferax mediterranei atcc33500.		1992	1378590
physical map of a 257 kilobase-pairs region from the genome of halococcus saccharolyticus.		1992	1633973
halobacterial a-atp synthase in relation to v-atpase.	the head piece separated from the a-atp synthase of halobacterium halobium hydrolyses atp. this a1-atpase is inhibited by nitrate but not by other chaotropic anions. the nitrate inhibition is noncompetitive with respect to atp, reversible, and partially protected by chloride. in contrast, atp synthase in situ (a1ao-atpase) is not inhibited by nitrate but apparently is inhibited by stronger chaotropic reagents, such as thiocyanate and trichloroacetate, which make the vesicle membrane permeable to ...	1992	9874757
on the revised structure of the major phospholipid of halobacterium salinarium.	recent fast atom bombardment-mass spectrometry (fabms) studies (tsujimoto, k., yorimitsu, s., takahashi, t. and ohashi, m. (1989) j. chem. commun. 668-670; frederickson, h.l., de leeuw, j.w., tas, a.c., van der greef, j., lavos, g.f. and boon, j.j. (1989) biomed. environ. mass. spectrom. 18, 96-105; kloppel, k.d. and fredrickson, h.l. (1991) j. chromatogr. 562, 369-376) have indicated that the structure of the major phospholipid of halobacterium salinarium (formerly halobacterium cutirubrum) is ...	1993	8334149
partial sequence of the gene for a serine protease from a halophilic archaeum haloferax mediterranei r4, and nucleotide sequences of 16s rrna encoding genes from several halophilic archaea.	a part of the gene coding for a halophilic serine protease from a halophilic archaeum haloferax mediterranei r4 was amplified by pcr and its 672 nucleotide sequence was determined. tentative translation to the amino acid sequence suggested that the enzyme was quite similar to halolysin produced by another halophilic archaeum strain 172p1. nucleotide sequences of 16s rrna encoding genes from 9 halophilic archaea were determined. alignment of 19 sequences known so far showed that there are more th ...	1993	8335078
comparative analysis of ribonuclease p rna structure in archaea.	although the structure of the catalytic rna component of ribonuclease p has been well characterized in bacteria, it has been little studied in other organisms, such as the archaea. we have determined the sequences encoding rnase p rna in eight euryarchaeal species: halococcus morrhuae, natronobacterium gregoryi, halobacterium cutirubrum, halobacteriurn trapanicum, methanobacterium thermoautotrophicum strains deltah and marburg, methanothermus fervidus and thermococcus celer strain al-1. on the b ...	1996	8614627
an archaea 5s rrna analog is stably expressed in escherichia coli.	mini-genes for 5s-like rrna were constructed. these genes had a sequence which largely resembles that of the naturally occurring 5s rrna of a bacterium, halococcus morrhuae, which phylogenetically belongs to the archaea. plasmids carrying the mini-genes were transformed into escherichia coli (ec). ribosomal incorporation was not a prerequisite for stable accumulation of the rna product. however, only those constructs with a well-base-paired helix i accumulated rna product. this result strongly i ...	1996	8626070
proposal to transfer halococcus turkmenicus, halobacterium trapanicum jcm 9743 and strain gsl-11 to haloterrigena turkmenica gen. nov., comb. nov.	the 16s rrna gene sequences of halococcus saccharolyticus and halococcus salifodinae were closely related (94.5-94.7% similarity) to that of halococcus morrhuae, the type species of the genus halococcus. however, halococcus turkmenicus was distinct from the other members of this genus, with low 16s rrna similarities when compared to halococcus morrhuae (88.7%). on the basis of phylogenetic tree reconstruction, detection of signature bases and dna-dna hybridization data, it is proposed to transfe ...	1999	10028254
effect of halococcus morrhuae and halobacterium saccharovorum on the activation of human peripheral blood lymphocytes.	the immunomodulator properties of two species of halophilic archaebacteria, halobacterium saccharovorum and halococcus rnorrhuae, were analysed by the study of lymphocyte activation. two methods were used to detect activation in lymphocytes, namely incorporation of the radioactive nucleotide [3h]-thymidine, and cd25 expression. h. morrhuae had a stimulatory effect on human lymphocytes, but this action was observed only with the [3h]-thymidine uptake method, whereas h. saccharovorum produced no i ...	1999	10464950
very similar strains of halococcus salifodinae are found in geographically separated permo-triassic salt deposits.	the authors have previously isolated a novel extremely halophilic archaeon, halococcus salifodinae blp, from austrian rock salt deposited about 250 million years ago. in this study they compared strain blp with two other halococci isolated independently from geographically distant salt deposits of similar age, and with two recent isolates (n1 and h2) from the same site as strain blp. strain bg2/2 was from a salt mine in germany and strain br3 from a halite deposit in england; both resembled hc. ...	1999	10627054
5s rrna fingerprints of marine bacteria, halophilic archaea and natural prokaryotic assemblages along a salinity gradient.	natural prokaryotic assemblages from two multi-pond solar salterns and pure cultures of both marine bacteria and halophilic archaea were analyzed and compared by electrophoretic analysis of 5s rrnas. a salinity gradient from seawater (3.7%) to nacl precipitation (37%) was studied. the culture-independent, pcr-free, fingerprinting analysis covered two objectives: (i) to compare natural assemblages among them and with results previously obtained through a pcr-dependent approach and (ii) to estimat ...	2000	11102688
different glycolytic pathways for glucose and fructose in the halophilic archaeon halococcus saccharolyticus.	the glucose and fructose degradation pathways were analyzed in the halophilic archaeon halococcus saccharolyticus by 13c-nmr labeling studies in growing cultures, comparative enzyme measurements and cell suspension experiments. h. saccharolyticus grown on complex media containing glucose or fructose specifically 13c-labeled at c1 and c3, formed acetate and small amounts of lactate. the 13c-labeling patterns, analyzed by 1h- and 13c-nmr, indicated that glucose was degraded via an entner-doudoroff ...	2001	11271421
mechanisms of acetate formation and acetate activation in halophilic archaea.	the halophilic archaea halococcus (hc.) saccharolyticus, haloferax (hf.) volcanii, and halorubrum (hr.) saccharovorum were found to generate acetate during growth on glucose and to utilize acetate as a growth substrate. the mechanisms of acetate formation from acetyl-coa and of acetate activation to acetyl-coa were studied. hc. saccharolyticus, exponentially growing on complex medium with glucose, formed acetate and contained adp-forming acetyl-coa synthetase (adp-acs) rather than acetate kinase ...	2001	11409546
novel haloarchaeal 16s rrna gene sequences from alpine permo-triassic rock salt.	prokaryotic diversity in alpine salt sediments was investigated by polymerase chain reaction (pcr) amplification of 16s rrna genes, sequencing of cloned products, and comparisons with culturable strains. dna was extracted from the residue following filtration of dissolved permo-triassic rock salt. fifty-four haloarchaeal sequences were obtained, which could be grouped into at least five distinct clusters. similarity values of three clusters to known 16s rrna genes were less than 90%-95%, suggest ...	2001	11523891
halogenated metabolites from the new okinawan red alga laurencia yonaguniensis.	a novel brominated diterpene based on the rare neoirieane skeleton, named neoirietetraol (1), has been isolated along with a halogenated c15 acetogenin, (3z)-laurenyne (2), from a new laurencia species, l.yonaguniensis masuda et abe, species inedita, collected at yonaguni island, okinawa prefecture, japan. the structures of these metabolites were elucidated by spectroscopic data (ir, 1h nmr, 13c nmr, 2d nmr, and ms). neoirietetraol (1) was toxic to the brine shrimp (altemia salina; lc50, 40.1 mi ...	2002	11908989
halococcus dombrowskii sp. nov., an archaeal isolate from a permian alpine salt deposit.	several extremely halophilic coccoid archaeal strains were isolated from pieces of dry rock salt that were obtained three days after blasting operations in an austrian salt mine. the deposition of the salt is thought to have occurred during the permian period (225-280 million years ago). on the basis of their polar-lipid composition, 16s rrna gene sequences, cell shape and growth characteristics, the isolates were assigned to the genus halococcus. the dna-dna reassociation values of one isolate, ...	2002	12361290
archaeosomes varying in lipid composition differ in receptor-mediated endocytosis and differentially adjuvant immune responses to entrapped antigen.	archaeosomes prepared from total polar lipids extracted from six archaeal species with divergent lipid compositions had the capacity to deliver antigen for presentation via both mhc class i and class ii pathways. lipid extracts from halobacterium halobium and from halococcus morrhuae strains 14039 and 16008 contained archaetidylglycerol methylphosphate and sulfated glycolipids rich in mannose residues, and lacked archaetidylserine, whereas the opposite was found in methanobrevibacter smithii, me ...	2003	15803661
structural changes in the cells of some bacteria during population growth: a fourier transform infrared-attenuated total reflectance study.	structural changes occurring in the cells of several bacteria during their growth curves have been investigated by fourier transform infrared (ft-ir) spectroscopy using the sampling technique of attenuated total reflectance (atr). spectra reflect all of the components of the cells, including the cell walls, cell membranes, internal structures, and the cytoplasm. the bacteria studied were bacillus stearothermophilus, halobacterium salinarum, halococcus morrhuae, and acetobacter aceti. all species ...	2004	15035713
identification of archaea and some extremophilic bacteria using matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) mass spectrometry.	archaea and a number of groups of environmentally important bacteria, e.g., sulfate-reducing bacteria, anoxygenic phototrophs, and some thermophiles, are difficult to characterize using current methods developed for phenotypically differentiating heterotrophic bacteria. we have evaluated matrix-assisted laser desorption/ionization time-of-flight (maldi-tof-ms) as a rapid method for identifying different groups of extremophilic prokaryotes using a linear mass spectrometer (micromass, uk). the ins ...	2004	15042434
[biochemical and genetic bases of chloroethene-dehalorespiring bacteria].		2005	16218455
[characterization of communities of heterotrophic bacteria associated with healthy and diseased corals in nha trang bay (vietnam)].	a comparative investigation of the heterotrophic microflora of 11 species of healthy corals and of white-band-diseased and yellow-band-diseased corals inhabiting the reefs of nha trang bay (vietnam), which has been exposed to anthropogenic impact, was performed. fifty-nine strains of heterotrophic bacteria isolated on y/k and endo media were investigated and characterized. all the isolates were identified at the genus level by consideration of the results of analysis of their phenotypic properti ...	2005	16315986
siderophores of halophilic archaea and their chemical characterization.	nine halophilic archaea viz., halobacterium salinarum, halobacterium sp.1, halobacterium sp.2, halobaculum sp., halococcus saccharolyticus, halorubrum saccharovorum, haloterrigena turkmenica, halogeometricum sp. and natrialba sp. isolated from marine salterns around bhavnagar coast were screened for siderophore production. five isolates viz., halococcus saccharolyticus, halorubrum saccharovorum, haloterrigena turkmenica, halogeometricum sp. and natrialba sp. produced siderophores as evidenced by ...	2006	16629380
phylogenetic relationships within the order halobacteriales inferred from 16s rrna gene sequences.	phylogenetic relationships within the halophilic archaea were inferred from comparisons of the 16s rrna gene sequences from 61 strains, representing 18 genera with validly published names within the order halobacteriales. trees produced using distance-matrix (least-squares and neighbour-joining) methods affirm with strong bootstrap support that the members of the order halobacteriales are a monophyletic group. ten genera were supported as monophyletic groups [haloarcula, halobiforma, halococcus, ...	2006	16738095
halococcus hamelinensis sp. nov., a novel halophilic archaeon isolated from stromatolites in shark bay, australia.	several halophilic archaea belonging to the genus halococcus were isolated from stromatolites from hamelin pool, shark bay, western australia, collected during field trips in 1996 and 2002. this is the first incidence of halophilic archaea being isolated from this environment. stromatolites are biosedimentary structures that have been formed throughout the earth's evolutionary history and have been preserved in the geological record for over 3 billion years. the stromatolites from hamelin pool, ...	2006	16738110
halococcus qingdaonensis sp. nov., a halophilic archaeon isolated from a crude sea-salt sample.	a gram-negative, extremely halophilic, coccoid archaeal strain, cm5(t), was isolated from a crude sea-salt sample collected near qingdao, china. the organism grew optimally at 35-40 degrees c and ph 6.0 in the presence of 20 % (w/v) nacl. its colonies were red in colour and it could use glucose as a sole carbon source for growth. the 16s rrna gene sequence of cm5(t) was most closely related to those of halococcus species. its pattern of antibiotic susceptibility was similar to those of other des ...	2007	17329792
a traditional japanese-style salt field is a niche for haloarchaeal strains that can survive in 0.5% salt solution.	most of the haloarchaeal strains have been isolated from hypersaline environments such as solar evaporation ponds, salt lakes, or salt deposits, and they, with some exceptions, lyse or lose viability in very low-salt concentrations. there are no salty environments suitable for the growth of haloarchaea in japan. although natrialba asiatica and haloarcula japonica were isolated many years ago, the question, "are haloarchaea really thriving in natural environments of japan?" has remained unanswere ...	2007	17346353
carotenoid analysis of halophilic archaea by resonance raman spectroscopy.	recently, halite and sulfate evaporate rocks have been discovered on mars by the nasa rovers, spirit and opportunity. it is reasonable to propose that halophilic microorganisms could have potentially flourished in these settings. if so, biomolecules found in microorganisms adapted to high salinity and basic ph environments on earth may be reliable biomarkers for detecting life on mars. therefore, we investigated the potential of resonance raman (rr) spectroscopy to detect biomarkers derived from ...	2007	17723094
halococcus thailandensis sp. nov., from fish sauce in thailand.	fifteen strains of red-pigmented, strictly aerobic, coccoid, extremely halophilic archaea were isolated from fish sauce (nam-pla) produced in thailand. they grew optimally at 37 degrees c, ph 6-8 and in the presence of 20-30 % (w/v) nacl. the dna g+c contents of the isolates were 60.0-61.8 mol%. they had mk-8(h2) as a major menaquinone component and c(20)c(20) and c(20)c(25) derivatives of phosphatidylglycerol, phosphatidylglycerol methylphosphate and a sulfated glycolipid, s-dga-1, as major pol ...	2007	17911282
lysis efficiency of standard dna extraction methods for halococcus spp. in an organic rich environment.	the extraction of nucleic acids from a given environment marks a crucial and essential starting point in any molecular investigation. members of halococcus spp. are known for their rigid cell walls, and are thus difficult to lyse and could potentially be overlooked in an environment. furthermore, the lack of a suitable lysis method hinders subsequent molecular analysis. the effects of six different dna extraction methods were tested on halococcus hamelinensis, halococcus saccharolyticus and halo ...	2008	18087671
analysis of yeast and archaeal population dynamics in kimchi using denaturing gradient gel electrophoresis.	kimchi is a traditional korean food that is fermented from vegetables such as chinese cabbage and radish. many bacteria are involved in kimchi fermentation and lactic acid bacteria are known to perform significant roles. although kimchi fermentation presents a range of environmental conditions that could support many different archaea and yeasts, their molecular diversity within this process has not been studied. here, we use pcr-denaturing gradient gel electrophoresis (dgge) targeting the 16s a ...	2008	18562030
investigating the effects of simulated martian ultraviolet radiation on halococcus dombrowskii and other extremely halophilic archaebacteria.	the isolation of viable extremely halophilic archaea from 250-million-year-old rock salt suggests the possibility of their long-term survival under desiccation. since halite has been found on mars and in meteorites, haloarchaeal survival of martian surface conditions is being explored. halococcus dombrowskii h4 dsm 14522(t) was exposed to uv doses over a wavelength range of 200-400 nm to simulate martian uv flux. cells embedded in a thin layer of laboratory-grown halite were found to accumulate ...	2009	19215203
emended descriptions of genera of the family halobacteriaceae.	the family halobacteriaceae currently contains 96 species whose names have been validly published, classified in 27 genera (as of september 2008). in recent years, many novel species have been added to the established genera but, in many cases, one or more properties of the novel species do not agree with the published descriptions of the genera. authors have often failed to provide emended genus descriptions when necessary. following discussions of the international committee on systematics of ...	2009	19244452
occurrence of halococcus spp. in the nostrils salt glands of the seabird calonectris diomedea.	the nostrils of the seabird calonectris diomedea are endowed with a salt-excreting gland that could produce a suitable environment for the colonization of extreme halophilic prokaryotes. we have studied in this organ the presence of extreme halophiles by means of culturing techniques. we could easily cultivate members of haloarchaea, and all cultures studied were identified as members of one of the two species halococcus morrhuae and hcc. dombrowskii. in order to reveal the diversity of these co ...	2009	19363644
natronoarchaeum mannanilyticum gen. nov., sp. nov., an aerobic, extremely halophilic archaeon isolated from commercial salt.	strain ysm-123(t) was isolated from commercial salt made from japanese seawater in niigata prefecture. optimal nacl and mg(2+) concentrations for growth were 4.0-4.5 m and 5 mm, respectively. the isolate was a mesophilic and slightly alkaliphilic haloarchaeon, whose optimal growth temperature and ph were 37 °c and ph 8.0-9.0. phylogenetic analysis based on 16s rrna gene sequence analysis suggested that strain ysm-123(t) is a member of the phylogenetic group defined by the family halobacteriaceae ...	2010	19965994
oil phytoremediation potential of hypersaline coasts of the arabian gulf using rhizosphere technology.	the rhizosphere and phyllosphere of the halophyte halonemum strobilaceum naturally inhabiting hypersaline coastal areas of the arabian gulf harbor up to 8.1 x 10(4)g(-1) and 3 x 10(2)g(-1), respectively, of extremely halophilic oil-utilizing microorganisms. such organisms were 14- to 38-fold more frequent in the rhizosphere than in the plant-free soil. frequent genera in the rhizosphere were affiliated to the archaea halobacterium sp. and halococcus sp., the firmicute brevibacillus borstenlensis ...	2010	20303746
biodegradation of crude oil and pure hydrocarbons by extreme halophilic archaea from hypersaline coasts of the arabian gulf.	two extreme halophilic haloferax strains and one strain each of halobacterium and halococcus were isolated from a hypersaline coastal area of the arabian gulf on a mineral salt medium with crude oil vapor as a sole source of carbon and energy. these archaea needed at least 1 m nacl for growth in culture, and grew best in the presence of 4 m nacl or more. optimum growth temperatures lied between 40 and 45 degrees c. the four archaea were resistant to the antibiotics chloramphenicol, cycloheximide ...	2010	20364355
identification of polyhydroxyalkanoates in halococcus and other haloarchaeal species.	polyhydroxyalkanoates (phas) are accumulated in many prokaryotes. several members of the halobacteriaceae produce poly-3-hydroxybutyrate (phb), but it is not known if this is a general property of the family. we evaluated identification methods for phas with 20 haloarchaeal species, three of them isolates from permian salt. staining with sudan black b, nile blue a, or nile red was applied to screen for the presence of phas. transmission electron microscopy and (1)h-nuclear magnetic resonance spe ...	2010	20437233
molecular assessment of uvc radiation-induced dna damage repair in the stromatolitic halophilic archaeon, halococcus hamelinensis.	the halophilic archaeon halococcus hamelinensis was isolated from living stromatolites in shark bay, western australia, that are known to be exposed to extreme conditions of salinity, desiccation, and uv radiation. modern stromatolites are considered analogues of very early life on earth and thus inhabitants of modern stromatolites, and hcc. hamelinensis in particular, are excellent candidates to examine responses to high uv radiation. this organism was exposed to high dosages (up to 500 j/m(2)) ...	2010	21074452
responses of haloarchaea to simulated microgravity.	various effects of microgravity on prokaryotes have been recognized in recent years, with the focus on studies of pathogenic bacteria. no archaea have been investigated yet with respect to their responses to microgravity. for exposure experiments on spacecrafts or on the international space station, halophilic archaea (haloarchaea) are usually embedded in halite, where they accumulate in fluid inclusions. in a liquid environment, these cells will experience microgravity in space, which might inf ...	2011	21417742
osmoadaptive strategies of the archaeon halococcus hamelinensis isolated from a hypersaline stromatolite environment.	abstract biogenic stromatolites are sources of significant information on the evolution of microbial life. despite their evolutionary significance, little is known about the mechanisms of osmoadaptation by microorganisms that comprise living stromatolites thriving in hypersaline environments. osmoadaptive strategies for halococcus hamelinensis, a novel halophilic archaeon recently isolated from living stromatolites in the hypersaline reaches of shark bay, were thus a particular interest in this ...	2011	21810017
mercury resistance and volatilization by oil utilizing haloarchaea under hypersaline conditions.	the hydrocarbon utilizing haloarchaea, haloferax (two strains), halobacterium and halococcus from a hypersaline coastal area of the arabian gulf, had the potential for resistance and volatilization of hg(2+). individual haloarchaea resisted up to between 100 and 200 ppm hgcl₂ in hydrocarbon free media with salinities between 1 and 4 m nacl, but only up to between 20 and 30 ppm in a mineral medium containing 3 m nacl, with 0.5% (w/v) crude oil, as a sole source of carbon and energy. halococcus an ...	2011	21061030