Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| disease risks associated with free-ranging wild boar in saskatchewan. | this study investigated the disease status of saskatchewan's feral wild boar population. whole carcasses, tissue samples, and/or serum from 81 hunter-killed boars from saskatchewan were submitted to the canadian wildlife health cooperative (cwhc) between 2009 and 2014. serological tests were negative for prrs, h1n1, and h3n2 swine influenza, pcv-2, and tge/prcv in 22/22 boars and for toxoplasma gondii and mycoplasma hyopneumoniae in 20/20 boars. of 20 boars whose sera were tested 20 were positiv ... | 0 | 26246630 |
| exposure of feral swine (sus scrofa) in the united states to selected pathogens. | feral swine (sus scrofa) are widely distributed in the united states. in 2011 and 2012, serum samples and tonsils were recovered from 162 and 37 feral swine, respectively, in the us to evaluate exposure to important swine endemic pathogens. antibodies against porcine reproductive and respiratory syndrome virus (prrsv) and porcine circovirus type 2 (pcv2) were found in 2.5% and 25.3% of tested sera, respectively. positive serological reactions against mycoplasma hyopneumoniae and actinobacillus p ... | 0 | 25673913 |
| new species: mycoplasma hyopneumoniae; a causative agent of virus pig pneumonia. | 1965 | 14323369 | |
| production of enzootic pneumonia in pigs with mycoplasma suipneumoniae grown in embryonated hens' eggs. | 1968 | 4886625 | |
| attempts to recover mycoplasma suipneumoniae from experimental and natural cases of enzootic pneumonia in pigs. | 1968 | 5250180 | |
| demonstration of complement-fixation antibody against mycoplasma hyopneumoniae in the sera of pigs infected with swine enzootic pneumonia. | 1968 | 5753358 | |
| production of pneumonia in gnotobiotic pigs with pure cultures of mycoplasma hyopneumoniae. | 1969 | 5813138 | |
| mycoplasma suipneumoniae isolated in denmark. | 1969 | 4901485 | |
| pneumonia in gnotobiotic pigs produced by simultaneous inoculation of a swine adenovirus and mycoplasma hyopneumoniae. | 1969 | 4304460 | |
| enzootic pneumonia in pigs: identification of a causative mycoplasma in infected pigs and in cultures by immunofluorescent staining. | immunofluorescent staining has been used to identify mycoplasma hyopneumoniae in smears of broth cultures, in infected pig testicle cell cultures, and in frozen cut sections of pneumonic lungs from field and experimentally produced cases of enzootic pneumonia. in the pneumonic pig lung, fluorescent staining was limited to the surface of the bronchial and bronchiolar epithelium and to the contained exudate. in a series of trials using experimentally infected pigs fluorescence was not detected unt ... | 1970 | 4246002 |
| the passive haemagglutination test for th detection of mycoplasma suipneumoniae and the possible diagnosis of enzootic pneumonia of pigs. | fourteen cases of enzootic pneumonia, nearly all of which had presented diagnostic difficulties using the metabolic-inhibition test, were re-examined using specific pig antisera in the passive haemagglutination test (pha). all proved positive for mycoplasma suipneumoniae, indicating that the test, used in this manner, might be particularly valuable for routine diagnosis.the pha test was also used to demonstrate antibody to m. suipneumoniae in pneumonic tissue and the associated bronchial lymph n ... | 1970 | 4988048 |
| serological studies in pigs with mycoplasma hyopneumoniae. | 1970 | 4988847 | |
| further observations on the problem of isolating mycoplasma suipneumoniae from field cases of enzootic pneumonia in pigs. | in previous work in this laboratory, mycoplasma suipneumoniae was recovered in liquid medium from 13% of individual cases and 18% of outbreaks of enzootic pneumonia in pigs. in the work now described, however, these recovery rates, when judged by the same criteria, were 45 and 75%, respectively. as there was evidence to suggest that this second series of pneumonic cases was less suitable for cultural examination than the first series, some of the other factors that might have improved the recove ... | 1970 | 5270206 |
| tube agglutination test of mycoplasma hyopneumoniae infection in swine. | 1970 | 5462579 | |
| direct isolation of mycoplasma suipneumoniae on solid medium from pneumotic lesions in pigs. | 1970 | 5531194 | |
| use of the complement fixation test in the demonstration of antibodies against mycoplasma suipneumoniae. | 1971 | 5150127 | |
| a selective medium for mycoplasma suipneumoniae. | 1971 | 5156237 | |
| selective isolation of mycoplasma suipneumoniae from pneumonic lesions in pigs. | 1971 | 5167328 | |
| sensitivity of mycoplasma suipneumoniae to penicillin-g. | 1971 | 5206577 | |
| the action of certain antibiotics and ether on swine enzootic pneumonia. | the susceptibility of mycoplasma hyopneumoniae to the action of three antibiotics and diethyl ether was determined. infected swine were used in an in vivo sensitivity detection system. the parameter of susceptibility was lesion prophylaxis. in vivo, mycoplasma hyopneumoniae appeared to be resistant to diethyl ether, tylosin tartrate, and erythromycin, but was susceptible to the action of chlortetracycline. chlortetracycline was effective in preventing the development of lesions when given at lev ... | 1971 | 4251409 |
| swine enzootic pneumonia: age susceptibility and treatment schemata. | pigs were found to be susceptible to mycoplasma hyopneumoniae-induced swine enzootic pneumonia (sep) when four hours old. chlortetracycline was incapable of preventing transmission of sep from infected pigs on the drug to susceptible, untreated pigs. when continuous medication was started at one or two weeks postinoculation, chlortetracycline partially or completely inhibited formation of sep lesions but did not clear m. hyopneumoniae from inoculated pigs. chlortetracycline administered orally w ... | 1971 | 4251420 |
| mycoplasma suipneumoniae and mycoplasma hyorhinis demonstrated in pneumonic pig lungs by the fluorescent antibody technique. | 1971 | 4102500 | |
| the survival of mycoplasma suipneumoniae in liquid medium, on solid medium and in pneumonic tissue. | 1972 | 5036322 | |
| isolation of mycoplasma suipneumoniae from the nasal cavities and lungs of pigs affected with enzootic pneumonia or exposed to this infecion. | 1972 | 5043128 | |
| response of swine to mycoplasma hyopneumoniae infection. | 1973 | 4689629 | |
| effect of mycoplasma hyopneumoniae infections, pyrantel treatment and protein nutrition on performance of pigs exposed to soil containing ascaris suum ova. | 1973 | 4703718 | |
| experimental infections of gnotobiotic piglets with pasteurella septica (sero-group a) alone or with mycoplasma hyopneumoniae. | 1973 | 4727890 | |
| an indirect haemagglutination test for detection of antibodies against mycoplasma hyopneumoniae using formalinized tanned swine erythrocytes. | 1973 | 4728117 | |
| preliminary studies on the cell-mediated immune response in pigs to mycoplasma hyopneumoniae. | 1973 | 4770238 | |
| enzootic pneumonia of pigs: immunization attempts inoculating mycoplasma suipneumoniae antigen by various routes and with different adjuvants. | 1973 | 4770241 | |
| experimental infection of pigs with mycoplasma hyopneumoniae (suipneumoniae). | 1974 | 4820079 | |
| an indirect haemagglutination test for detection of antibodies against mycoplasma hyopneumoniae using formalinized tanned swine erythrocytes. | 1974 | 4850551 | |
| tube agglutination tests with mycoplasma hyopneumoniae and m. hyorhinis. | 1974 | 4475607 | |
| evaluation of some serological techniques for the identification of mycoplasma hyorhinis and mycoplasma suipneumoniae. | 1974 | 4604451 | |
| some recommendations concerning primary isolation of mycoplasma suipneumoniae and mycoplasma flocculare a survey. | two mycoplasma species of the porcine respiratory tract: mycoplasma suipneumoniae and mycoplasma flocculare, have been notoriously difficult to cultivate. in the present paper a description is given of a new medium with which primary isolation of these organisms is usually successful. the basal medium is made from commercial dehydrated products. pig serum is added and phenol red used as ph indicator. contrary to what is customary in the preparation of mycoplasma media, various inorganic salts (h ... | 1975 | 1098011 |
| [mycoplasms of the swine--a review]. | the mycoplasmas constitute a group of microorganisms placed between bacteria and virus. the name, mycoplasma, is derived from the mycelial morphology of the organisms. the minimal reproductive unit, the elementary body, measures 0.2-0.5 mum. unlike bacteria, mycoplasmas are not confined by a rigid cell wall, but just by a thin membrane. for their cultivation, though common bacteriological technique is adequate, especially enriched media are required. antibiotics, as a rule penicillins, are added ... | 1975 | 1153279 |
| detection of mycoplasma hyopneumoniae antibodies in porcine serum by complement-fixation test. | a total of 660 porcine serum samples from 35 herds in 6 states were tested for complement-fixing antibodies against mycoplasma hyopneumoniae. samples were prepared from blood collected from swine herds suspected of having mycoplasmal pneumonia because of clinical signs or lesions or both. only 56 serums from 10 herds gave positive test results. | 1975 | 1163882 |
| isolation of mycoplasma hyopneumoniae and its association with pneumonia of pigs in australia. | nine strains of mycoplasmas were isolated from the lungs of 5 pigs with clinical signs of naturally acquired enzootic pneumonia. mycoplasma hyopneumoniae was isolated from the lungs of 1 pig and m. hyorhinis from the lungs of 4. an unidentified mycoplasma, which utilized arginine, grew rapidly in broth culture and produced centred colonies on solid medium, was isolated from the lungs of 4 pigs. the pathogenicity of the isolated strain of m. hyopneumoniae was determined by inoculation of pigs fro ... | 1975 | 1164268 |
| the effects of mycoplasma hyorhinis and of mycoplasma hyopneumoniae on pig kidney primary tissue culture. | 1975 | 1192170 | |
| an improved medium for the isolation of mycoplasma suipneumoniae. | 1976 | 944489 | |
| a new method for the demonstration of antibodies against mycoplasma suipneumoniae in pigs sera. | 1976 | 969191 | |
| microflora of pneumonic lungs in a pig herd established by hysterectomy. | mycoplasmas and other micro-organisms are recorded from a pig herd originally derived by hysterectomy. complement fixation tests were positive for mycoplasma hyopneumoniae 20 months after the first hysterectomy. following a marked increase in pneumonic lesions found at slaughter, and after modifying the culture medium m hyopneumoniae was isolated. the principal micro-organism associated with this mycoplasma was pasteurella multocida. | 1976 | 130669 |
| a fluorescent antibody technique for identification of mycoplasma hyopneumoniae colonies. | fluorescent antibody staining of mycoplasma hyopneumoniae colonies is difficult because the colonies wash from the agar. airdrying of the colonies grown on solid medium-overlayed glass microscope slides fixed the colonies in place, so that fluorescent antibody stain could be readily accomplished. apparent loss or alteration of antigenicity did not result from the air-drying process. the technique is useful for the identification of m hyopneumoniae isolates. | 1976 | 773225 |
| scanning electron microscopy of trachea and bronchi from gnotobiotic pigs inoculated with mycoplasma hyopneumoniae. | areas of trachea and right apical and right dorsal diaphragmatic bronchi from gnotobiotic neonatal pigs were examined by scanning electron and transmission electron microscopy at 7, 13, and 21 days after pigs were given intranasal inoculation of mycoplasma hyopneumoniae. similar areas were examined by scanning electron microscopy from 2 noninoculated neonatal pigs. tracheal and bronchial lesions were observed in all inoculated pigs. severe bronchial lesions were in grossly affected lobes. early ... | 1977 | 911092 |
| apparent re-infection of enzootic-pneumonia-free pig herds: specificity of diagnosis. | in a control scheme for enzootic-pneumonia-free herds, 43 herds developed enzootic pneumonia, as judged by non-specific clinical and pathological criteria over 10 years. material for cultural examination was obtained from 32 of these outbreaks, and mycoplasma suipneumoniae was identified in 28 of them. the four failures occurred before 1971 at a time when the cultural techniques were clearly inferior. since 1971 m suipneumoniae has been isolated from all the 17 outbreaks sampled for this purpose ... | 1977 | 595285 |
| [immunological technics for the determination of mycoplasma suipneumoniae in frozen sections and bronchial smears]. | 1977 | 323968 | |
| mycoplasma suipneumoniae and mycoplasma flocculare in the growth precipitation test. | 1977 | 327769 | |
| quantitative detection of antibodies to mycoplasma suipneumoniae in pigs' sera by an ezyme-linked immunosorbent assay. | a new method for quantitative detection of antibodies to mycoplasma suipneumoniae in pigs' sera has been developed by the application of an enzyme-linked immunosorbent assay (elisa). the results show that the elisa is specific and highly sensitive. in experimentally infected pigs antibodies could be detected several weeks before the clinical manifestation of enzootic pneumonia. | 1977 | 333714 |
| utilisation of glucose by mycoplasma suipneumoniae and mycoplasma flocculare. | 1978 | 358810 | |
| the immunological response of pig bronchial lymph node to mycoplasma suipneumoniae. | 1978 | 621308 | |
| sensitivity in vitro to macrolide antibiotics and tetracyclines of mycoplasma hyopneumoniae isolated from pneumonic porcine lungs. | 1978 | 652044 | |
| cytopathogenicity of mycoplasma hyopneumoniae in porcine tracheal ring and lung explant organ cultures alone and in combination with monolayer cultures of fetal lung fibroblasts. | porcine tracheal rings and lung explants alone and in combination with monolayer cultures of porcine lung fibroblasts (plf) were separately inoculated with virulent strains of mycoplasma hyopneumoniae and incubated at various times. the preparations were observed by bright-field, phase-contrast, and scanning electron microscopy. in plf cultures, the strains at initial concentrations of 10(1.3) colony-forming units/ml increased within 3 days to 10(6) colony-forming units/ml, showed progressive cl ... | 1978 | 669807 |
| in vitro susceptibility of mycoplasma hyopneumoniae and mycoplasma hyorhinis to fifty-one antimicrobial agents. | by a tube dilution assay technique, 51 antimicrobial agents were singly tested against 9 strains of mycoplasma hyopneumoniae and 1 strain of m. hyorhinis for the purpose of obtaining information useful for selecting an agent for testing in vivo against porcine mycoplasmal pneumonia. based on determining minimal inhibitory concentrations and chemically grouping the agents into nine classes, all m. hyopneumoniae strains were found resistant to penicillins and peptides and susceptible to sulfonamid ... | 1978 | 697348 |
| attempts to demonstrate cell-mediated immune response during mycoplasma suipneumoniae infection of pigs. | measurements of lymphocyte transformation and skin sensitivity were used to investigate whether pigs previously inoculated with mycoplasma suipneumoniae would show a t-lymphocyte-mediated immune response. in the lymphocyte transformation test blood lymphocytes, obtained at various intervals after intranasal inoculation of m suipneumoniae cultures, were incubated with specific antigen and the degree of transformation was assessed by 14c-thymidine uptake. reaction to intradermal antigen was assess ... | 1978 | 749085 |
| influence of a mycoplasma respiratory tract infection on sheep erythrocyte and complement receptor-bearing cell composition in the respiratory tract of swine. | pulmonic and peripheral blood lymphocyte subpopulations in healthy and mycoplasma suipneumoniae-infected pigs were compared. the t- and the b-lymphocyte populations were counted, the b cells by the complement receptor (zymosan-complement rosettes) technique and the t cells by the ed-rosette technique (sheep rbc-dextran rosettes). the t cells were found to predominate among pulmonic, as well as blood, lymphocytes. pulmonic b-cell and blood t-cell percentages were increased after mycoplasma respir ... | 1979 | 316662 |
| [studies on erythrocyte-rosette-forming peripheral blood cells in pigs infected with mycoplasma hyopneumoniae]. | 1979 | 420696 | |
| resistance of mycoplasma hyopneumoniae to chlortetracycline. | 1979 | 435212 | |
| evaluation of diagnostic procedures for detection of mycoplasmal pneumonia of swine. | the complement-fixation test (cft), a latex-agglutination test (lat), and mycoplasma hyopneumoniae isolation procedures were compared with gross and microscopic lung evaluations for the routine diagnosis of mycoplasmal pneumonia (mp) of swine. the difficulty and variability of m hyopneumoniae isolation rendered this technique unacceptable. in market-weight swine, the finding of typical gross lesions was correlated with cft results (p less than 0.005). the mp status of a herd could not be determi ... | 1979 | 447548 |
| activity of tiamulin against mycoplasma suipneumoniae and enzootic pneumonia of pigs. | 1979 | 452356 | |
| isolation of mycoplasma hyopneumoniae from lesions in experimentally infected pigs. | pigs aged 6 to 9 weeks from enzootic pneumonia-free herds were inoculated intranasally with a suspension of pneumonic lung containing mycoplasma hyopneumoniae or were placed in contact with such inoculated pigs. all the inoculated pigs had gross lesions of enzootic pneumonia when killed 27 to 42 days after inoculation. the culture methods described enabled m. hyopneumoniae to be isolated from all 29 inoculated pigs. of 45 pigs in contact with inoculated pigs 35 had gross lesions of enzootic pneu ... | 1979 | 533486 |
| tween 20 soluble proteins of mycoplasma hyopneumoniae as antigen for an enzyme linked immunosorbent assay. | the preparation of an antigen of mycoplasma hyopneumoniae containing selected proteins after treatment with the neutral detergent tween 20 and purification on sephadex g25 is described. the antigenic activity and the specificity were determined by means of an enzyme linked immunosorbent assay and compared with that of a whole-cell, sds-solubilised antigen. the tween 20 purified antigen showed a comparable activity but a higher specificity. | 1980 | 6166972 |
| a complement-fixation test for enzootic pneumonia of pigs using a complement dilution method. | complement-fixing antibody to mycoplasma hyopneumoniae in the serums of pigs experimentally infected with enzootic pneumonia was demonstrated by comparing the haemolytic titre of guinea-pig complement titrated in the presence of heated test serum, m. hyopneumoniae antigen and unheated normal pig serum with the titre obtained when the antigen was omitted. the haemolytic titres against sensitised sheep erythrocytes were determined after a fixation period of 16 to 18 hours at 5 degrees c. when seru ... | 1980 | 7436902 |
| duration of mycoplasma hyopneumoniae infection in gnotobiotic pigs. | sixteen gnotobiotic pigs raised in flexible plastic isolators (four pigs per isolator) were inoculated with a culture of mycoplasma hyopneumoniae. one pig was killed and underwent necropsy at weekly intervals for the following 16 weeks. macroscopic lesions were observed in the lungs of 13 of 16 pigs and microscopic lesions were found in 14 of 16 pigs. mycoplasma hyopneumoniae was cultured from the trachea or lungs from 10 of the 16 pigs. scanning electron microscope studies showed areas of damag ... | 1980 | 7438008 |
| medicated early weaning to obtain pigs free from pathogens endemic in the herd of origin. | a field trial was conducted to assess the value of medicated early weaning for obtaining pigs free from some of the pathogens endemic in their herd of origin. the trial comprised 51 sows from a closed herd, which were farrowed in an isolated farrowing house in seven separate groups. the sows in each group were bred at the same time and induced to farrow on the same day. their thriftiest piglets were weaned at five days of age and moved to an isolated early-weaning unit. at about six weeks of age ... | 1980 | 7445326 |
| production of arthritis by intravenous inoculation of mycoplasma hyopneumoniae: tests on five strains. | cultures of five strains of mycoplasma hyopneumoniae (syn, m suipneumoniae) were each inoculated intravenously into four pigs aged eight to nine weeks. one strain produced no response. for two strains two of the four pigs showed complement fixing antibody and mycoplasmaemia and one of these pigs in each group developed arthritis in one limb joint. the response to the other two strains was more severe: all four pigs in each group had a serological response, were mycoplasmaemic and several limb jo ... | 1981 | 7244376 |
| an abattoir survey of the incidence of pneumonia in saskatchewan swine and an investigation of the microbiology of affected lungs. | the lungs of 15 409 pigs, mostly from saskatchewan, slaughtered at an abattoir were examined over a one year period. the incidence of lesions was 36.7% for "enzootic" pneumonia and 2.1% for pleurisy unassociated with pneumonia. seasonal variations were recorded and compared with the results of similar surveys carried out in australia, belgium and england. mycoplasmological examination of lungs from 347 animals was consistently negative for mycoplasma hyopneumoniae. pasteurella multocida was the ... | 1981 | 7248880 |
| cell-mediated and humoral immune response in swine after vaccination and natural infection with mycoplasma hyopneumoniae. | litters of pigs were vaccinated with inactivated mycoplasma hyopneumoniae organisms in freund's complete adjuvant. the cell-mediated and humoral immune response following vaccination was investigated, and the protective effect of the induced immunity was assayed. the cell-mediated immune response to whole m hyopneumoniae organisms was determined as incorporation of tritiated thymidine, using a microculture system. after vaccination, detectable sensitization of peripheral blood lymphocytes was in ... | 1981 | 7258797 |
| comparison of a microtitration complement-fixation test and a tube latex agglutination test for diagnosis of mycoplasma hyopneumoniae swine pneumonia. | comparison of a tube latex agglutination (la) test and a microtitration complement-fixation (cf) test for the diagnosis of mycoplasma hyopneumoniae swine pneumonia was done. in a group of 107 pigs from iowa, texas, and california (survey a), correlation of data on microscopic pulmonary changes with the la and cf titers indicated the la test might be a better indicator of microscopic lesions than the cf test. in another field study involving lungs from 50 pigs from various herds (survey b), both ... | 1981 | 7258806 |
| [pleuropneumonia in swine due to haemophilus pleuropneumonia s. parahaemolyticus. ii. studies on the epidemiology and the relation to chronic pleuritis (pleural scars) in baconers (author's transl)]. | within a danish practice area a serological examination was made of 268 breeding animals in 25 conventional sow herds with a view to screening for herd infection with haemophilus pleuropneumoniae. average size of the herds was 76 year-sows including pregnant gilts (8--200). in 23 herds produced weaners were fed for slaughtering. the frequency of chronic pleuritis in slaughtered baconers in these herds was related to the frequency of seropositive sows in the herds. three spf-herds were included i ... | 1981 | 7322864 |
| the pathological and serological response induced in pigs by parenteral inoculation of mycoplasma hyopneumoniae. | 1981 | 7343577 | |
| effects of mycoplasma hyopneumoniae and m. hyorhinis on ependymal cells of the porcine lateral ventricles as observed by scanning and transmission electron microscopy. | porcine lateral ventricular ependymal cells were used to study the effects of infection with mycoplasma hyopneumoniae and m. hyorhinis on the central nervous system (cns) of pigs. after cerebral or cerebrospinal inoculation, cellular sequential changes were observed by scanning (sem) and transmission (tem) electron microscopy. strains of m. hyopneumoniae and m. hyorhinis were recovered from infected tissues at 1 and 3 weeks postinoculation. the mycoplasmas caused lesions of ependymal cells. dege ... | 1981 | 7347417 |
| interaction of mycoplasma hyopneumoniae with the porcine respiratory epithelium as observed by electron microscopy. | an in vivo-passaged strain of mycoplasma hyopneumoniae attained viability titers of 10(6) to 10(8) color-changing units per mg of tissue in pig lungs and caused gross and histological pulmonic lesions. mycoplasmas were readily located in the lumina of the respiratory tract by electron microscopy. in sections of tissue fixed in glutaraldehyde-osmium, the organisms were found to possess many radial fibrils on the outer surface of the limiting membrane. these fibrils appeared to interconnect adjace ... | 1982 | 7129633 |
| tylosin tartrate and tiamutilin effects on experimental piglet pneumonia induced with pneumonic pig lung homogenate containing mycoplasmas, bacteria and viruses. | the effects of tylosin tartrate and tiamutilin were examined in pneumonias induced experimentally in neonatal piglets with a homogenate of pneumonic pig lung, obtained from pigs with naturally acquired enzootic pneumonia. the homogenate contained mycoplasmas, including mycoplasma hyopneumoniae (m suipneumoniae) and m hyorhinis, and certain bacteria and viruses. the experimental pneumonias generally resembled mycoplasmal pneumonia histologically but were complicated by aspiration pneumonia in som ... | 1982 | 7134653 |
| a method for assessing induced resistance to enzootic pneumonia of pigs. | the resistance induced in pigs by the intravenous, intraperitoneal and subcutaneous inoculation of live mycoplasma hyopneumoniae was tested by placing inoculated pigs in contact with pigs artificially infected with enzootic pneumonia. control pigs inoculated by the same routes with sterile culture medium were exposed simultaneously to the infected pigs and the incidence and extent of the pneumonia in both groups were compared. a score derived from the cube root of the calculated or estimated vol ... | 1982 | 7146627 |
| cytopathic effect of whole cells and purified membranes of mycoplasma hyopneumoniae. | marked cytopathic effect was produced in both porcine lung fibroblasts and mrc-5 human lung fibroblasts with doses of 7.5 x 10(6) and 1.5 x 10(7) cfu of mycoplasma hyopneumoniae j per ml, respectively. m. hyopneumoniae vpp11, at a concentration as low as 1.25 x 10(7) cfu/ml, was capable of causing cytopathic effect in mrc-5 monolayers also. the infected monolayers developed patches of detached or lysed cells within 48 h. at a protein concentration of 2 micrograms/ml, m. hyopneumoniae membranes, ... | 1983 | 6862623 |
| prevalence of antibodies to mycoplasma hyopneumoniae in iowa swine. | the prevalence of complement-fixing antibodies to mycoplasma hyopneumoniae was determined in 7,321 sera collected from breeding swine of various ages. samples were selected randomly in approximate proportion to the number of hogs marketed annually from each of 9 crop-reporting areas in iowa. testing was accomplished by means of a microtiter complement-fixation test. of the 7,321 sera, 22% had antibody titers of 1:4 or greater to m hyopneumoniae. of the 597 herds sampled, 60% or 357 had at least ... | 1983 | 6638651 |
| tiamulin feed medication for the maintenance of weight gains in the presence of mycoplasmal pneumonia in swine. | tiamulin, a semisynthetic antibiotic, was fed to growing pigs and its value in maintaining weight gain and feed efficiency in the face of mycoplasmal pneumonia was determined. four treatments divided among 64 growing pigs from a conventional pig farm were used to test the effects of tiamulin administered in feed at levels of 10, 20 and 30 ppm. all pigs were artificially infected with mycoplasma hyopneumoniae. medication was started on the 14th day after infection (d 0) and continued for 28 d (d ... | 1983 | 6674286 |
| delayed hypersensitivity to nonviable mycoplasma pulmonis in mice is enhanced by dextran sulfate. | the enhancing effect of dextran sulfate (ds) on delayed hypersensitivity to nonviable mycoplasma pulmonis in mice was evaluated by means of delayed footpad swelling. mice pretreated subcutaneously with nonviable m. pulmonis and ds had significantly increased delayed footpad swelling when the mice were injected subcutaneously in the footpad with the same antigen 7 days later. delayed footpad swelling was not detected in mice pretreated only with nonviable m. pulmonis or ds followed by footpad inj ... | 1983 | 6187687 |
| comparison of mycoplasma hyopneumoniae strains by serologic methods. | six field strains of mycoplasma hyopneumoniae isolated from pneumonic lungs of pigs, reference strains 11 and j of m hyopneumoniae, ms 42 strain of mycoplasma flocculare, and bts 7 strain of mycoplasma hyorhinis were compared serologically, using hyperimmune antisera produced in rabbits. all strains of m hyopneumoniae were closely related as determined with the disk growth-inhibition test; however, differences in zone sizes indicated that some antigenic heterogeneity existed. cross-reactions wer ... | 1983 | 6196995 |
| improvement of complement fixation test antigen for the diagnosis of mycoplasma hyopneumoniae infection. | several attempts were made to develop a complement fixation test antigen which is useful and practicable for the diagnosis of mycoplasmal pneumonia of swine. for the preparation of antigen, mycoplasma hyopneumoniae was grown in broth medium containing horse serum for 14 days by shaking culture. anticomplementary activity of antigen was eliminated by addition of complement and heating at 56 degrees c for 30 minutes. storage of this antigen at 4 degrees c or at room temperature for 12 months had n ... | 1983 | 6240607 |
| comparison of manually performed microtiter plate and semiautomated (cuvette) indirect enzyme-linked immunosorbent assays for the serodiagnosis of mycoplasmal pneumonia of swine. | standardized enzyme-linked immunosorbent assays for the serodiagnosis of mycoplasmal pneumonia of swine were developed, using the traditional microtiter plate method and a method based on semiautomated processor-analyzer instrumentation. the results of the 2 procedures were not significantly different when the titers of positive and negative anti-mycoplasma hyopneumoniae standard reference sera were determined in triplicate in 3 separate experiments. although both methods yielded reproducible re ... | 1983 | 6354018 |
| comparison of the enzyme-linked immunosorbent assay and the indirect hemagglutination and complement fixation tests for detecting antibodies to mycoplasma hyopneumoniae. | caesarean-derived, colostrum-deprived swine were exposed to a broth culture of a low passage field isolate of mycoplasma hyopneumoniae by intranasal inoculation. the intranasal-inoculated swine subsequently were commingled with their litter-mates to effect transmission via contact-exposure. sera were collected from the swine at two to four week intervals for approximately one year postexposure and evaluated by the enzyme-linked immunosorbent assay (elisa), indirect hemagglutination and complemen ... | 1983 | 6365294 |
| diagnosis of mycoplasmal pneumonia of swine: sequential study by direct immunofluorescence. | an improved direct fluorescent antibody test was evaluated for specificity and efficacy in diagnosing mycoplasmal pneumonia of swine. a sequential study was carried out in which pigs inoculated with a pneumonic lung suspension containing mycoplasma hyopneumoniae strain 11 were euthanatized at postinoculation (pi) weeks 2 to 12. fluorescent coating of m hyopneumoniae was detected primarily on bronchial and bronchiolar epithelial surfaces of lungs with gross lesions of pneumonia. fluorescence was ... | 1984 | 24049896 |
| serological cross-reactivity of porcine reference antisera to mycoplasma hyopneumoniae, m. flocculare, m. hyorhinis and m. hyosynoviae indicated by the enzyme-linked immunosorbent assay, complement fixation and indirect hemagglutination tests. | the enzyme-linked immunosorbent assay (elisa) indicated significant cross-reactivity between the antigens of mycoplasma hyopneumoniae ( hyop ) and m. flocculare (floc), another porcine mycoplasma of wide distribution but uncertain pathogenic significance, when porcine antisera of each specificity were tested against hyop antigen. the titers of the anti-floc sera ranged from threefold to 13-fold less than the titer of the anti- hyop reference serum at different times after immunization. these val ... | 1984 | 6372971 |
| comparison of complement fixation test and enzyme-linked immunosorbent assay for detection of early infection with mycoplasma hyopneumoniae. | the relative merits of the complement-fixation test (cf) and enzyme-linked immunosorbent assay (elisa) for the detection of the early antibody response to mycoplasma hyopneumoniae were evaluated. discriminant analysis, a statistical procedure, was used to avoid difficulties associated with variation in background color and nonspecific reactions obtained with elisa with different sera. specific-pathogen-free pigs were exposed by contact to other specific-pathogen-free pigs which had been inoculat ... | 1984 | 6377989 |
| evaluation of the indirect hemagglutination assay as a practical serodiagnostic test for mycoplasmal pneumonia of swine. | sera from swine experimentally or naturally infected with mycoplasma hyopneumoniae (the etiological agent of mycoplasmal pneumonia of swine, mps) were tested by the indirect hemagglutination assay (iha), the enzyme-linked immunosorbent assay (elisa) and the complement fixation (cf) test. the iha detected antibody at comparable times and levels to the other 2 serological tests following experimentally-induced infection. in the late antibody response (greater than or equal to 86 days post-infectio ... | 1984 | 6433540 |
| evaluation of criteria for the postmortem diagnosis of mycoplasmal pneumonia of swine. | ten swine from each of five herds believed to be affected with mycoplasmal pneumonia of swine and ten swine from each of five herds believed to be mycoplasmal pneumonia-free were selected for postmortem study. lungs from the 100 swine were examined; grossly and microscopically for lesions typical of mycoplasmal pneumonia of swine and culturally and by an indirect immunofluorescent procedure for the presence of mycoplasma hyopneumoniae. nineteen of the lungs had both gross and microscopic lesions ... | 1984 | 6434167 |
| detection of antibodies against membrane-glycoproteins of swine erythrocytes after experimental infection with mycoplasma hyopneumoniae. | during enzootic pneumonia of pig, antibodies against membrane glycoproteins of erythrocytes were detected with an enzyme-linked immunosorbent assay (elisa). these antibodies arise simultaneously with the specific antibodies. they are not identical to cold agglutinins. | 1984 | 6485250 |
| apparent reinfection of enzootic-pneumonia-free pig herds: early signs and incubation period. | since 1959, the pig health control association (phca) has run a national health-control scheme for pig herds believed to be free from enzootic pneumonia. during this time, many herds developed this disease without a simple explanation. from 1968, 55 such unexplained breakdowns have been studied in detail. the first signs in 50 breakdowns were either coughing in growing pigs (52 per cent of outbreaks), illness in adult stock (34 per cent of outbreaks) or pneumonia in routinely slaughtered pigs (1 ... | 1984 | 6495592 |
| characteristics of protective activity of mycoplasma hyopneumoniae vaccine. | protective activity of mycoplasma hyopneumoniae vaccines prepared from whole cells or crude extracts was evaluated in an experimentally induced mycoplasmal pneumonia swine model. swine were obtained at 7 to 10 weeks of age from a surgically derived herd free of porcine mycoplasmas. vaccines prepared from whole cells, freeze-thaw-saline solution extract, supernate of culture, or saline wash of cells were treated with 0.15% formalin, incorporated with freund incomplete adjuvant, and administered i ... | 1984 | 6497088 |
| assessment of a complement fixation test to detect mycoplasma hyopneumoniae infection in pigs. | serums from pigs slaughtered at abattoirs were tested for evidence of mycoplasma hyopneumoniae infection using a complement fixation (cf) test which avoids the procomplementary effect of pig serum. to establish a diagnosis of enzootic pneumonia, the lungs from all sampled pigs were examined for pathological and histological changes consistent with the disease and cultures were made for mycoplasmas and bacteria. the study was carried out at parkville and bendigo 160 km apart at different times an ... | 1984 | 6497806 |
| effect of mycoplasma hyopneumoniae infection on the development of haemophilus pleuropneumoniae pneumonia in pigs. | 1984 | 6513243 | |
| mycoplasma hyopneumoniae infection in pigs immunosuppressed by thymectomy and treatment with antithymocyte serum. | the effect of immunosuppression on mycoplasma hyopneumoniae infection was evaluated by comparing data from infected, thymectomized, and antithymocyte serum-treated pigs (group 1) with data from infected (group 2) and noninfected (group 3) healthy pigs. after groups 1 and 2 pigs were inoculated intranasally with m hyopneumoniae, mycoplasmas tended to multiply slightly more in the lungs and bronchial lymph nodes of group 1 pigs than that of group 2 pigs. organisms were also isolated from the splee ... | 1984 | 6333838 |
| pulmonary and hematologic changes in swine with mycoplasma hyopneumoniae pneumonia. | studies were made of the physiologic changes in the lung of young growing pigs intratracheally inoculated at 5.5 to 6 weeks of age with mycoplasma hyopneumoniae. hematologic changes also were studied. studies were performed at the 4th, 5th, and 6th weeks after inoculations were done. the inoculated pigs had a marked decrease in body weight gain. there were no significant changes in the heart rate, respiratory rate, and rectal temperature, even though at necropsy the apical, cardiac, and diaphrag ... | 1984 | 6711976 |
| effect of age on susceptibility of pigs to mycoplasma hyopneumoniae pneumonia. | the effect of age on susceptibility of pigs to mycoplasma hyopneumoniae was compared in 2 experiments. in experiment a, pigs 3, 6, and 12 weeks of age were contact-exposed to pigs with established m hyopneumoniae infection for 27 days and then evaluated for pneumonia at necropsy done at 42 days after exposure began. in experiment b, pigs 3 and 11 to 12 weeks of age were contact-exposed for 20 days and then placed in separate, isolated hog houses until necropsy was done at 49 days after exposure ... | 1984 | 6711977 |
| reproducible pneumonia in gnotobiotic piglets induced with broth cultures of mycoplasma hyopneumoniae and the effect of animal passage on virulence. | the virulence of a laboratory adapted culture of mycoplasma hyopneumoniae strain nb12 was determined in three- to five-day-old gnotobiotic piglets. intranasal inoculation or exposure to an aerosol of the culture caused low incidences of pneumonia in the piglets. passage of m hyopneumoniae strain nb12 in gnotobiotic piglets resulted in a rapid increase in virulence. after only three in vivo passages, severe pneumonia involving most lobes of the lung developed in all inoculated piglets within thre ... | 1984 | 6718814 |
| surface proteins of mycoplasma hyopneumoniae identified from an escherichia coli expression plasmid library. | a genomic library of mycoplasma hyopneumoniae was constructed by cloning random dna fragments approximately 300 base pairs long in a fusion expression plasmid, pex29, containing the n terminus of the phage ms2 polymerase under the control of the pl promoter of phage lambda. clones that produced fusion proteins carrying surface-specific antigenic determinants were identified by using antiserum raised in a pig by intranasal inoculation of viable mycoplasmas. rabbit antisera produced against gel-pu ... | 1985 | 2410363 |
| effect of enzootic pneumonia of pigs on growth performance. | growing pigs were naturally infected with a field strain of mycoplasma hyopneumoniae to assess the effect of enzootic pneumonia on production. both the initial ("breakdown") and endemic stages of infection were evaluated. the pigs were reared under environmental and management conditions commonly found on commercial piggeries in south australia. growth rate of pigs held in-contact with inoculated pigs was reduced by 12.7% (p less than 0.01) between 50 to 85 kg bodyweight. in the second trial ino ... | 1985 | 3924012 |
| cell-mediated and humoral immune response to mycoplasma hyopneumoniae in pigs enhanced by dextran sulfate. | the effect of dextran sulfate (ds), known to be cytotoxic to macrophages, on the cell-mediated and humoral immune response to nonviable mycoplasma hyopneumoniae in pigs was investigated. the cell-mediated immune response was determined by means of lymphocyte transformation a test, using uptake of [3h]thymidine in a microculture system and the humoral immune response by means of a microplate complement-fixation test. peripheral blood lymphocytes from pigs vaccinated with nonviable m hyopneumoniae ... | 1985 | 2581484 |
| stimulation of immunoglobulin-containing cells and isotype-specific antibody response in experimental mycoplasma hyopneumoniae infection in specific-pathogen-free pigs. | the development of immunoglobulin-containing cells and antigen-specific plasma cells in retropharyngeal and bronchial lymph nodes, in lung tissue, and in nasal mucous membrane was followed during an experimental infection of pigs with mycoplasma hyopneumoniae. an enzyme-linked immunosorbent assay was used to follow stimulation of the immunoglobulin isotypes (classes) m, g, and a directed against the infectious agent in tracheobronchial secretions and systemically. a significant increase of antig ... | 1985 | 3161830 |