Publications

thermoactinomyces tsiklinsky, a genus of thermophilic actinomycetes.		1953	13096490
production and isolation of thermoviridin, an antibiotic produced by thermoactinomyces viridis n. sp.		1956	13303133
[phage of a thermophilic micromonospore--micromonospora vulgaris].		1961	13681676
studies on the thermophilic actinomycetes. i. methods of cultivation.	a total of 1,000 isolates of thermophilic actinomycetes representing two genera, streptomyces and thermoactinomyces, were studied. media for cultivation and for physiological studies were designed. differences between the two genera are noted and taxonomic criteria for the genus thermoactinomyces are suggested. the importance of the nutritional environment to the thermophilic habit is noted.	1961	13775873
studies on peat and peat microorganism. i. taxonomic studies on thermophilic actinomycetes isolated from peat.		1963	13927630
antigenic structure of the actinomycetales. vii. chemical and serological similarities of cell walls from 100 actinomycetales strains.	kwapinski, j. b. (the university of new england, armidale, australia). antigenic structure of the actinomycetales. vii. chemical and serological similarities of cell walls from 100 actinomycetales strains. j. bacteriol. 88:1211-1219. 1964.-cell walls prepared mechanically from 100 strains of actinomycetales were studied by chromatographic and serological methods. the cell walls of actinomyces were found to be serologically related to those of the corynebacteria and to some strains of mycobacteri ...	1964	14234773
chemical composition of cell-wall preparations from strains of various form-genera of aerobic actinomycetes.	cell-wall preparations were made from more than 140 strains of aerobic actinomycetes representing most of the form-genera that have been proposed. all cell-wall preparations contained as major constituents glucosamine, muramic acid, alanine, and glutamic acid. in addition, cell-wall preparations from various types of streptomycetes and strains of microëllobosporia contained glycine and ll-alpha,epsilon-diaminopimelic acid; those from strains of most actinoplanaceae and micromonosporae contained ...	1965	14325886
isolation of amylolytic strains of thermoactinomyces vulgaris and production of thermophilic actinomycete amylases.	kuo, m. j. (iowa state university, ames), and p. a. hartman. isolation of amylolytic strains of thermoactinomyces vulgaris and production of thermophilic actinomycete amylases. j. bacteriol. 92:723-726. 1966.-of 759 isolates obtained from dung, compost, and soil samples, a culture of thermoactinomyces vulgaris (strain 5) was selected for further study on the basis of quantities of amylase produced in synthetic and nonsynthetic media, rapid growth and sporulation, culture stability upon prolonged ...	1966	5922543
purification and partial characterization of thermoactinomyces vulgaris amylases.		1967	6054676
purification and properties of proteolytic enzymes from thermophilic actinomycetes.	the enzymes isolated from two selected cultures of thermophilic actinomycetes-thermomonospora fusca (a 29) and thermoactinomyces vulgaris (a 60)-possess proteolytic activity. the enzymes were purified more than 35- to 40-fold and showed three bands each upon cellulose acetate electrophoresis at several ph values. based upon sephadex gel filtration, molecular weights of 21,500 and 23,800 were calculated for the active peaks of the enzymes. the purified enzymes lysed heat-killed cells of gram-posi ...	1969	5344092
phages of lysogenic thermoactinomyces vulgaris.		1969	4190294
immunofluorescence identification of thermopolyspora polyspora, the causative agent of farmer's lung.	farmer's lung is a serious disabling pulmonary disease found in agricultural workers. the disease is believed to be a hypersensitivity to the thermophilic actinomycetes, principally thermopolyspora polyspora. this organism is difficult to stain with the usual bacteriological stains and thus far has not been demonstrated in the lung tissue by microscopic methods. in this paper, it is demonstrated that the fluorescent-antibody technique is a simple method for the positive identification of t. poly ...	1969	4976327
precipitins with relevance to farmer's lung and aspergillosis in normal and other sera.	immunodiffusion, immunoosmophoresis, and precipitin inhibition tests were carried out on various categories of sera with extracts of micropolyspora faeni (thermopolyspora polyspora), aspergillus fumigatus, and thermoactinomyces vulgaris. the sera were from clinical cases of farmer's lung, from symptomless farm workers, from expectant mothers, from tuberculous and from asthmatic patients, and from patients with miscellaneous lung disorders. the clinical cases of farmer's lung became divisible int ...	1969	4983353
genetic recombination in a thermophilic actinomycete, thermoactinomyces vulgaris.		1970	5500021
thermoactinomyces sacchari sp. nov., a thermophilic actinomycete causing bagassosis.		1971	4999215
[spin-label study of the characteristics of spore activation in thermoactinomyces vulgaris 2681].		1971	4331833
spore germination in thermophilic actinomycetes. i. preliminary observations with thermoactinomyces vulgaris and actinobifida dichotomica.		1972	4679114
structure of thermorubin a, the major orange-red antibiotic of thermoactinomyces antibioticus.		1972	5032502
[luminescence of thermoactinomyces vulgaris colonies in relation to spore formation and spore germination].		1972	5043518
[sensitivity of the vegetative mycelium of thermoactinomyces vulgaris to external factors].		1972	5044901
amylolytic isoenzymes of thermoactinomyces vulgaris.	thermoactinomyces vulgaris strain 5 produced two electrophoretically different alpha-amylases. precipitation with ammonium sulfate and acetone did not alter the electrophoretic mobilities of either amylase isoenzyme. patterns of the hydrolysis products of amylose by the two amylase isoenzymes were essentially identical.	1972	5057774
proteases produced by thermoactinomyces albus.		1972	5078890
[rings in colonies of thermoactinomyces vulgaris in relation to changes in temperature and relative humidity].		1972	5084517
spore germination in thermophilic actinomycetes. ii. germinal changes in water suspensions of thermoactinomyces vulgaris spores.		1972	4121998
effect of different levels of propionic acid on the growth of micropolyspora faeni (a.94) and thermoactinomyces vulgaris (a.64).		1973	4763680
[does thermal treatment always activate the sporulation of thermoactinomyces vulgaris?].		1973	4791159
[conditions for the initiation of thermoactinomyces vulgaris spores].		1973	4792253
[epr signals from spin-labeled activated spores of thermoactinomyces vulgaris 2681].		1973	4357457
[effect of cooling on the germination of thermoactinomyces vulgaris spores].		1974	4407450
[control of thermoactinomyces vulgaris endospore release from dormancy].		1974	4449492
spore initiation and minimum temperature for growth of thermoactinomyces vulgaris.		1974	4464658
spore initiation and minimum temperature for growth of thermoactinomyces vulgaris.		1974	4599716
antibody responses in patients with farmer's lung disease to antigens from thermoactinomyces vulgaris.	a serological analysis of mycelial antigens of thermoactinomyces vulgaris in immunodiffusion with human sera revealed five individual antigens. three antigens were proteins, sensitive to pronase and soluble in phenol. two were cationic polysaccharides, sensitive to sodium periodate, and containing glucosamine and muramic acid. latex coated with mycelial antigens was compared with precipitin tests in detecting antibodies to t. vulgaris; the number of positive results detected by each test differe ...	1975	803522
high incidence of precipitins in sera of heroin addicts.	we analyzed sera of 50 consecutively hospitalized heroin-abuse patients for precipitins against several antigens. forty-seven addicts had precipitins against extracts from moldy hay, and 34 against extracts from bagasse. thirty-six showed precipitin bands against extracts from one or more of the following: aspergillus, micropolyspora faeni, and thermoactinomyces vulgaris. these findings were significantly different from those of a control population (p less than .0001 for all antigens tested). ...	1975	805851
[development of thermoactinomyces vulgaris on a synthetic medium].	a synthetic medium was used to obtain the dormant spores of thermoactinomyces vulgaris. the fraction of the dormant spores depended on the amino acid composition of the growth medium. the rate of growth and development of the organism on the synthetic medium is lower as compared to the routinely employed complex medium.	1975	1177783
use of a serologically distinct strain of thermoactinomyces vulgaris in the diagnosis of farmer's lung disease.	during investigations into farmer's lung disease it was noticed that micropolyspora faeni (mf) was isolated and identified with ease, but difficulty was encountered in the identification of thermoactinomyces vulgaris (tv), although an organism resembling tv was frequently isolated. extracts prepared from the isolates resembling tv (called thermoactinomyces vulgaris variant, tvv) when tested against the standard tv antiserum by double diffusion, did not produce any precipitin lines. when tvv extr ...	1975	1206112
[temperature regimes for the formation and germination of thermoactinomyces vulgaris endospores].		1975	1206150
[effect of the composition of the medium and magnesium and calcium ions on the germination of spores of thermoactinomyces vulgaris].	the germination of the spores of thermoactinomyces vulgaris formed on a complex medium is stimulated by suspending them in solutions containing mg2+ and ca2+ ions. the stimulation is not the result of the initiation of the spores in the presence of the ions since the experiments were carried out at a temperature of 20 degrees c at which the initiation did not virtually take place. the ions of na+ and k+ have almost no effect on the germination of the spores. the fraction of the resting spores of ...	1975	1214606
a scheme for the identification of thermophilic actinomycetes associated with hypersensitivity pneumonitis.	a scheme has been developed for the identification of thermophilic actinomycetes associated with hypersensitivity pneumonitis. eighty strains, 10 micropolyspora faeni, 6 saccharomonospora viridis, 52 thermoactinomyces candidus, 7 t. vulgaris, 4 t. sacchari, and 1 t. dichotomica, either isolated from patients' environment or received as authentic strains, were studied. in addition to the cultural and microscopic morphology in various media, each strain was subjected to an array of biochemical tes ...	1975	1225928
a quantitative study on the activation of the alternative pathway of complement by mouldy hay dust and thermophilic actinomycetes.	materials associated with the induction of farmer's lung were incubated with fresh normal human serum in the presence of magnesium ethylene glycol tetra-acetic acid (mgegta) or ethylene diamine tetra-acetic acid (edta) and results compared with material known to activate the alternative pathway of complement--zymosan. results show that micropolyspora faeni organisms are as active as zymosan in reducing complement (c) levels in the presence of mgegta, with a 50% reduction in ch50 at approximatel ...	1976	1248097
activation of the complement sequence by extracts of bacteria and fungi associated with hypersensitivity pneumonitis.	complement (c) consumption by antigens associated with hypersensitivity pneumonitis was investigated. micropolyspora faeni in the presence of precipitating antibodies consumed c in a manner similar to the bsa-anti bsa system. an extract of m. faeni was shown to consume c and convert c3pa to c3a in the absence of detectable antibodies. in addition, three species of aspergillus and two strains of thermoactinomyces vulgaris were also shown to have the same activity with respect to c3pa. mucor racem ...	1976	1262608
the value of precipitating antibodies in screening for hypersensitivity pneumonitis.	to evaluate the usefulness of precipitin tests as a screening method to detect hypersensitivity pneumonitis, we compared persons with precipitins to organic antigens with precipitin-negative subjects from the same population of 1,072 office workers participating in a health survey examination. thirty-three of the 53 persons with preciptins to micropolyspora faeni, thermoactinomyces vulgaris, t. candidus, pigeon serum, aspergillus, alternaria, pullularia, penicillium, cephalosporium, trichoderma, ...	1976	1267251
alkaloid from thermoactinomyces species.		1976	1269064
precipitating antibodies in a midwest dairy farming population toward the antigens associated with farmer's lung disease.	a survey of the frequency of precipitins to the antigens of the thermophilic actinomycetes and aspergillus species was conducted on serum samples from 1,045 farmers obtained at a 3-day exposition on modern farm equipment and farming practices in central wisconsin. each farmer filled out a questionnaire including socioeconomic information, lung disease history, exposure history, and smoking history. precipitins were detected by the double-diffusion method. the antigen panel included eight thermop ...	1976	932340
letter: thermoactinomyces in refrigerators.		1976	947042
the effect of respiratory immunization on cell-mediated immune effector cells of the lung.	in order to understand the mechanisms of cellular immune injury in hypersensitivity pneumonitis, the effect of type of antigen on cell-mediated immunity in guinea-pigs receiving respiratory immunization was studied. lymphocytes obtained by pulmonary lavage were compared with those from peritoneal exudate following immunization with either a soluble protein, human serum albumin, or a particulate suspension of thermoactinomyces vulgaris. assays were obtained without mixing cells from these two sou ...	1976	949869
[protein hydrolysis by immobilized enzymes].	the enzymic hydrolysis of some proteins (insulin-b-chain-s-sulfonate, s-aminoethylated lysozyme, bovine serum albumin) by immobilized peptidolytic enzymes is reported. sepharose-bound pronase, trypsin and a protease from thermoactinomyces sp. (mp), the latter both cross linked by glutaric dialdehyde and an exopeptidase mixture containing sepharose-bound leucine aminopeptidase, carboxypeptidase a and a crude preparation of prolidase were used. after enzymic hydrolysis nearly all amino acids, exce ...	1976	983621
home humidifier thermophilic actinomycete isolates.	twenty-seven thermophilic actinomycete isolates obtained from humidification systems or living-bedroom areas of individuals with suspected but unproved home environment-related respiratory disease were characterized morphologically and biochemically. all isolates were demonstrated to be members of the thermoactinomyces genus. two previously misidentified isolates were reclassified as a thermoacetinomyces sp. thus, all of our thermophilic actinomycete humidifier isolates studied to date have been ...	1976	1078519
immunologic cross-reactions among thermophilic actinomycetes associated with hypersensitivity pneumonitis.	antibodies produced in rabbits against micropolyspora faeni, thermoactinomyces vulgaris, t. sacchari, thermoactinomyces candidus, and saccharomonospora viridis were tested against antigens derived from many strains of thermophilic actinomycetes for precipitating antibodies by immunodiffusion test. it was found that immune sera reacted strongly against antigens from strains belonging to the same species and weakly against antigens from different species of thermophilic actinomycetes. however, ser ...	1976	816841
yeast cell wall-dissolving enzymes of the thermotolerant actinomycete thermoactinomyces vulgaris.	the thermotolerant culture of thermoactinomyces vulgaris pa ii-4a was cultivated in the biotec fermenter to obtain the enzyme preparation with a high proteolytic and lytic activity. resistance of the cells of candida utilis and saccharomyces fragilis (mesophilic and thermotolerant strains) to the lytic action of the enzyme preparation was different. preliminary treatment of the yeast cells by l-cysteine increased their susceptibility to the lytic action of the preparation. the degree of lysis of ...	1976	6860
[incorporation of thymidine into the dna of actinomycetes. i. incorporation of exogenous thymidine into the dna of thermoactinomyces vulgaris].	the incorporation of exogenous thymidine and thymine into acid-insoluble material of thermoactinomyces vulgaris has been studied during germination and subsequent growth. thymine is not incorporated. the incorporation of thymidine stops after a short time due to the rapid breakdown of thymidine to thymine and deoxyribose-1-phosphate by the inducible thymidine phosphorylase. deoxyadenosine enhances the incorporation of thymidine as well as of thymine and prolongs the tine of uptake. uridine stim ...	1977	304639
growth of thermoactinomyces.		1977	320356
indirect immunofluorescent detection of antibodies against thermophilic actinomycetes in patients with hypersensitivity pneumonitis.	with slide culture preparations of micropolyspora faeni, thermoactinomyces candidus, and thermoactinomyces vulgaris used as antigens, 59 sera from patients with hypersensitivity pneumonitis and 23 sera from normal controls were studied for the presence of specific antibodies by indirect immunofluorescence and immunodiffusion methods. of the 82 sera, 31 showed precipitins to m. faeni, five to t. candidus, and four to t. vulgaris. a group of 19 sera positive to aspergillus antigens showed question ...	1977	402432
epoxidation of aldrin to exo-dieldrin by soil bacteria.	twenty-two strains of soil bacteria, including representatives of the genera bacillus, micromonospora, mycobacterium, nocardia, streptomyces, thermoactinomyces, and pseudomonas and 10 unidentified gram-negative, motile, rod-shaped bacteria, were shown to degrade aldrin to its epoxide dieldrin. in every case, the exo-stereoisomer of dieldrin was produced exclusively.	1977	407844
[causes of degeneration of cultures of thermoactinomyces vulgaris].	passage of cultures of thermoactinomyces vulgaris on a peptone-maize medium causes their degeneration which is manifested in an impaired formation of the sporulating aerial mycelium, and in an increase of the amount of non-germinating spores in populations. the process of degeneration depends on the following conditions: the location of the inoculated material (spores) on the surface of a solid growth medium, which is determined by the technique of inoculation; the state of the spores (degenerat ...	1977	882014
[spores of degenerating cultures of thermoactinomyces vulgaris].		1977	882023
isolation of bacteriophage from thermoactinomyces.	bacteriophages were isolated from strains of thermoactinomyces vulgaris, t. candidus, and t. sacchari used to produce antigen for hypersensitivity pneumonitis screening at the marshfield medical foundation. whereas the one phage isolated from t. sacchari and two phages from t. vulgaris were species specific, three other phages isolated from t. vulgaris and the two phages isolated from t. candidus were infectious for both t. vulgaris and t. candidus, thus indicating a close relationship between t ...	1977	914995
studies on thermoactinomyces sp. tm9208. i. description of the strain.	a strain of thermoactinomyces sp., tm9208, isolated from a soil sample in the taipei area showed antagonistic activity to gram positive bacteria but not to the gram negative on the potato extract agar plate by the cross streaking method. the strain showed strong starch hydrolysis, beta type of hemolysis, brownish yellow growth on the potato extract agar and green on the nutrient agar. its aerial mycelium, white to grayish white, was long and straight with short branches. the spores are single an ...	1977	578130
production of thermophilic actinomycete-hay aerosols for use in experimental hypersensitivity pneumonitis.	an investigation of the factors that influence the production of dense aerosols of micropolyspora faeni and thermoactinomyces vulgaris from hay cultures revealed that the density, freshness, and moisture content of the hay were important influences. dry aerosols were produced under optimum conditions from hay cultures of either actinomycete and from sterile hay for inhalation challenges to unimmunized rabbits. depressions in arterial oxygen tensions and hemolytic complement were monitored after ...	1977	596873
[isolation, crystallization and partial characterization of a cationic protease from thermoactinomyces vulgaris].	a simple method was developed for the isolation of the cationic endopeptidase from a crude extract prepared from the culture medium of thermoactinomy ces vulgaris, consisting in chromatography on sephadex g 75 and subsequent separation on cm-sephadex in 50 mm tris/hcl, ph 8, using a nacl-gradient (0 - 0,2 m). this procedure results in a 4,2 fold increase of the elastolytic activity (substrate: n-acetyl-(l-ala)3methyl ester) of the enzyme. it moves as a single band in sds-gel electrophoresis and ...	1977	612086
elimination of bagassosis in louisiana paper manufacturing plant workers.	the prevalence of bagassosis was investigated in a louisiana paper mill, which in the past had considerable numbers of workers with the disease. based on negative clinical histories and the low number of positive serological reactions of the workers' serum with thermoactinomyces sacchari antigen, it was concluded that bagassosis was no longer present. this was thought to be due to a different method of storage of bagasse, which retards microbial decay and reduces airborne organic dust, and to th ...	1978	627039
[viability study of actinomycete cultures preserved for a long time on agar media].	viability of 254 actinomycetous strains stored for 1 to 6 years on agarized media a temperature of 4-10 degrees c without passages was studied. the cultures included 204 strain of actinomyces, 26 strains of mycobacterium, 2 strains of streptosporangium, 1 strain of intrasporangium, 2 strains of micropolyspora and 5 strains of thermoactinomyces. most of the cultures, i.e. 94.55 per cent stored for 6 years remained vialbe after the this period. with an increase in the storage period from 1 to 4 ye ...	1978	655683
characterization of thermoactinomyces sacchari antigens.	three different extracts of thermoactinomyces sacchari were analyzed for their antigenicity and physical-chemical properties. rabbit antiserum to each preparation, tested by immunodiffusion in gel, demonstrated that the most potent immunogen was that prepared by the double-dialysis method. this extract also contained the greatest number of precipitating antigens as detected by gel filtration. all extracts, analyzed by column chromatography on sephadex g-75, were heterogeneous in that they contai ...	1978	669809
isolation anc characterization of actinophages of thermoactinomyces and micropolyspora.	two new actionphages infecting thermophilic actinomycetes were isolated from lysogenic cultures of thermoactinomyces candidus and micropolyspora faeni. the physiochemical properties, morphology, host range, electron microscopy, and immunology of the two phages, phi-115a (atcc 29680-b1) and phi-150a (atcc 29681-b1), are reported.	1978	679066
[variability of the proteolytic activity in the thermotolerant actinomycete, thermoactinomyces vulgaris].	the thermophilic culture of thermoactinomyces vulgaris pa-11-4a, like other actinomycetes, is characterized by a high variability. this strain produces protease. the property is maintained if spores are incubated in sterilized distilled water for 30 min after lyophilization and then inoculated onto a solid medium (petri plates) in order to select small dark-coloured colonies. the activity of protease does not decrease if such colonies are grown on solid media during seven months at 4 degrees c.	1978	703646
transition of thermoactinomyces substrate mycelium from growth to sporulation.		1978	735168
[characterization of a protease from thermoactinomyces vulgaris (thermitase). 1. purification of thermitase].	the paper deals with the purification of the microbial protease preparation "thermitase" (submerged cultivation of thermoactinomyces vulgaris; treatment of culture filtrate with ethanol or na2so4, vacuum drying of precipitate). the crude substance was purified by column chromatography on sephadex g-75, deae-cellulose and sephadex g-50. the proteolytically active fractions were in each case united, freeze dried and tested for protein components and protease activity by gel electrophoresis. after ...	1978	749455
[characterization of a protease from thermoactinomyces vulgaris (thermitase). 2. single-step fine purification and protein-chemical characterization].	the fine purification of an alkaline protease (thermitase) from thermoactinomyces vulgaris by means of isoelectrical focussing in the flat-bed procedure using granulated gel is reported. an na2so4-precipitated crude product serves as the starting material. isoelectrical focussing leads in a single step to a highly purified protein with an uniform n-terminal end group. the enzyme has an ip at 9.0 and a mol. wt. of 37,400; it consists of a polypeptide chain with arginine as the n-terminal, and tyr ...	1978	749456
kinetics of vegetative growth of thermoactinomyces vulgaris.	the growth of thermoactinomyces vulgaris on solid media was studied by microculture technique. starting from a germinating spore the further development was followed by phase contrast microscopy. from serially taken photographs the kinetics of growth and branching of the mycelium was calculated. also some observations on morphological behaviour were made. regarding the total mycelium developing from one spore the growth occurred witha constant exponential growth rate, at least for the time teste ...	1978	753045
[the thermophilic streptomycetes flora in milk powders and condensed milk products (author's transl)].	247 specimens of powdered milk and 165 of condensed milk were tested for their contamination with thermophile streptomycetes. colonies of these contaminants were isolated from all specimens of powdered milk and from 73 samples of condensed sweeted milk. the isolated strains corresponded with the following species of thermophilic streptomycetes: thermoactinomyces vulgaris, tsiklinsky 1899, thermoactinomyces vulgaris, tsiklinsky 1899 "giant colonies", micromonospora sp. (agre et al., 1).	1978	581428
occurrence and thermoresistance of thermophilic streptomycetes spores in milk.	200 samples of raw milk and 100 samples of pasteurized milk were tested for the presence of thermophilic streptomycetes. one ml of raw and pasteurized milk contained mean 8.42 and 14.95 thermophilic streptomycetes, respectively. the isolated strains belonging to the species thermoactinomyces vulgaris tsiklinsky 1899 and micromonospore sp. (agre et al., 1972) were identified. to determine the survival of spores of these thermophiles after pasteurization, samples of milk were heated at 63.5 degree ...	1978	581429
[studies on the composting of feathers (author's transl)].	the nitrogen of keratin of feathers is not taken up by plants as a suitable n-source. it becomes available by the keratinolytic activity of microorganisms. the high temperature evolved during the composting process promotes the growth and activity of thermophilic, keratin-decomposing microorganisms, e.g. thermoactinomyces vulgaris. the increasing in temperature (self-heating) reflects the course of composting. a feather-peat mixture exhibits a considerably high heat production, i.e. a fast decom ...	1978	569407
the diagnostic value of antibodies to the traditional antigens of farmer's lung in finland.	precipitating antibodies towards antigens associated with farmer's lung disease were studied in finnish dairy farming and non-farming rural populations and the results were compared to those of serum samples of patients suspected of having a fungal allergy. the antigen panel consisted of aspergillus fumigatus, micropolyspora faeni and thermoactinomyces vulgaris. all three microbes seemed to be important environmental inducers of antibody formation. the prevalence of antibodies towards all of the ...	1978	361284
[production and characterization of proteases of thermoactinomyces vulgaris. i. proteases of thermophilic microorganisms (a review)].		1978	362740
lipase productivity of a lipolytic strain of thermoactinomyces vulgaris.	an attempt was made to evaluate the lipase productivity by thermoactinomyces vulgaris with various nutritional and environmental factors. results suggest optimum conditions for production, viz. age of inoculum 18 hours, 55 degrees c, ph 6.8, 0.2% yeast extract, specific substrates, and shaken cultures incubated for 24--36 hours. lipases are induced by corn oil in presence of yeast extract or of certain substitutes; starch could replace these at 55 degrees c. a suggestion is given of a regulatory ...	1978	34950
identity and lipase activity of an isolate of thermoactinomyces vulgaris.	an actinomycete with thermophilic nature and strong lipolytic activity was isolated by enrichment from an egyptian soil. it proved to be thermoactinomyces vulgaris. variabilities justifying adansonian systematics were reported and discussed. the potential relation of an occasional drop in successfully resumed activity to an auto-inhibition phenomenon has been notified. optimum conditions for the activity of the extracellular lipase(s), including stability, suggest the practical importance of the ...	1978	34951
[characterization of a protease from thermoactinomyces vulgaris (thermitase). 3. substrate specificity and properties of partially purified thermitase].	during the process of cultivation of th. vulgaris several proteases are formed. in the present investigation the extensively purified major component was used. the substrate specificity was determined by means of 7 proteins, 7 amino acid esters, 5 fatty acid esters and 15 amino acid 4-nitroanilides. among the protein substrates tested, urea denaturated hemoglobin was split best, followed by gelatin, casein, field bean protein, serum albumin and gluten. the weakest rate of hydrolysis was observed ...	1978	34957
cellulolytic enzyme system of thermoactinomyces sp. grown on microcrystalline cellulose.	the carboxymethyl-cellulase and avicelase activities of thermoactinomyces sp., strain yx, were produced simultaneously with cell growth. throughout the growth phase these activities were primarily extracellular, with up to 50% adsorbed to residual cellulosic substrate at any one time. on the other hand, the beta-glucosidase activity was associated with the culture solids throughout the entire fermentation and appears to be intracellular. preparative isoelectric focusing of the culture filtrate, ...	1978	16345322
[production and characterization of the proteases from thermoactinomyces vulgaris. iii. studies of protease formation in a small-scale pilot plant].		1979	35884
[production and characterization of proteases in thermoactinomyces vulgaris. iv. spectrum of extracellular proteases during cultivation].		1979	39384
chemical and serological comparison of two antigen extracts of thermoactinomyces candidus.		1979	86157
antigenic relationships among thermophilic actinomycetes.	antigens from micropolyspora faeni, saccharomonospora viridis, thermoactinomyces candidus, t. sacchari and t. vulgaris were prepared by growing them on dialysate of trypticase soy broth. sera from rabbits immunized with these antigens were used to study cross-reactivity between thermophilic actinomycetes by antigen-antibody crossed immunoelectrophoresis and by agar gel double diffusion. mi. faeni and s. viridis showed some degree of cross-reaction, but both failed to show any cross reactivity wi ...	1979	94466
association of beta-glucosidase with intact cells of thermoactinomyces.	the location of the b-glucosidase activity in a whole culture broth of the thermophilic organism thermoactinomyces has been studied. little beta-glucosidase activity was found in the culture filtrate, while the culture solids contained the major part of the activity of the whole culture broth. the activity does not appear to be adsorbed to the culture solids; rather there is evidence that it is an intracellular soluble enzyme(s). the ph and temperature optima for a crude beta-glucosidase prepara ...	1979	106906
humidifier-associated extrinsic allergic alveolitis.	three cases of allergic alveolitis due to indoor humdification systems are described. thermoactinomyces vulgaris precipitins were detected in the serum of a 37-year-old female patient who had typical febrile attacks during exposure to cool-mist from a home humidifier. when the cause was detected and eliminated, the symptoms and signs disappeared and the woman's gas transfer factor improved from 56% to normal within six months. in a printing office a 60-year-old woman had had febrile attacks with ...	1979	375386
[effect of oils and fatty acids on growth and enzyme production of thermoactinomyces vulgaris. i. effect of oils and fatty acids on the metabolism of microorganisms (review of literature)].		1979	397684
microbiological and serological studies of farmers' lung in finland.	sera from few finnish patients with clinical farmers' lung react in precipitin tests with extracts of the thermophilic actinomycetes that commonly cause the disease elsewhere. hays associated with the disease in finland showed less evidence of spontaneous heating and contained fewer actinomycete spores than british hays. only thermoactinomyces vulgaris was sometimes abundant. some species of mesophilic fungi were more abundant than in britain and one, aspergillus umbrosus, reacted with most sera ...	1979	421335
[production and the characterization of the proteases from thermoactinomyces vulgaris. ii. the selection of productive strains].		1979	442700
[substrate specificity of a protease from thermoactinomyces vulgaris].	the cleavage specificity of a protease from thermoactinomyces vulgaris (thermitase) was determined by the insulin b-chain and the cleavability of casein and haemoglobin by this enzyme as compared to other proteases (trypsin, chymotrypsin, proteases from bac. megaterium and cytophages). the most intense splitting effect on the substrates under investigation (insulin b-chain, casein and haemoglobin) is exerted by thermitase, i. e., the unspecificity of this enzyme is especially marked.	1979	460396
properties of thermoactinomyces vulgaris phage ta1 and its extracted dna.	the virulent phage ta1 was obtained in good yields from infected cultures of thermoactinomyces vulgaris 1227. the purified phage was found to sediment with a single band, the sedimentation constant being (519 +/- 14)s, and to exhibit a typical nucleoprotein behaviour in uv-spectrophotometric and cd experiments. the ta1 phage consists of a hexagonal head about 0.056 micrometers in diameter and a very short tail. it is morphologically similar to the temperate salmonella phage p22. the nucleic acid ...	1979	516794
crossed immunoelectrophoretic analysis of two antigen extracts of thermoactinomyces candidus.	a pyridine extract antigen and a double-dialysis antigen (dda) obtained from thermoactinomyces candidus were analyzed by crossed immunoelectrophoresis. in addition, the heat lability, pronase sensitivity, and isolectric points of the components of the dda were determined. by using antisera raised against crude pyridine extract antigen, two immunogenic components were resolved by crossed immunoelectrophoresis. a similar analysis of dda using antisera raised against crude dda revealed 15 immunogen ...	1979	528048
[lipolytic activity from milk isolated strains of the species thermoactinomyces vulgaris, tsiklinsky 1899 (author's transl)].	thirty strains of the species thermoactinomyces vulgaris (tsiklinsky, 1988) were isolated from milk and tested for their lipolythic activity. after incubation for 120 hours at 55 degrees c they formed distinct diffusion zones on culture media containing tributyrin and tween-80. in all strains exocellular lipases were found. their action on the milk fat increased the number of the free fatty acids. triglycerides, free fatty acids as well as diglycerides and monoglycerides were identified as split ...	1979	573533
phosphate utilization and constitutive synthesis of phosphatases in thermoactinomyces vulgaris tsilinsky.	thermoactinomyces vulgaris utilized both organic and inorganic phosphates with equal efficiency for its growth. the specific activities of the thermophilic acid and alkaline phosphatases were found to be maximum at 1 mm concentration of each phosphate source. all the phosphatase isoenzymes (three alkaline and one acidic) were observed irrespective of the substrate source and concentration, suggesting constitutive synthesis of the enzymes. during growth and differentiation, both acid and alkaline ...	1980	6258569
respiratory symptoms and lung function in a sample of vermont dairymen and industrial workers.	this study reviews the respiratory status of a sample of vermont male dairy farmers, and a comparison group from industry, matched for age, sex and smoking. survey instruments included a standardized questionnaire and simple pulmonary function tests. in general, past and present smokers had more respiratory symptoms than never-smokers; and farmers, in all smoking categories, reported symptoms with greater frequency than did their counterparts from industry. forced vital capacity (fvc) tended to ...	1980	7356085
transfection in thermoactinomyces vulgaris.		1980	7376689
the effect of thermophilic actinomycetes isolated from compost and animal manure on some strains of salmonella and shigella.	several samples of compost and animal manure were taken from different localities. eleven isolates of thermophilic actinomycetes were obtained. the morphological, cultural, and physiological properties as well as temperature relation of these isolates were carried out in some detail in order to classify and identify them. the 11 isolates were identified as follows: thermoactinomyces vulgaris (2 strains), streptomyces thermofuscus (5 strains) and thermoactinomyces sacchari (3 strains). the antiba ...	1980	7376752
[studies on the variability of a strain of thermoactinomyces vulgaris, tsilinsky, 1899, which splits into sectors].	studies on the variability of the characteristics of air mycelium and physiological and biochemical properties of the colonies issued from a strain of thermoactinomyces vulgaris. this strain had air mycelium split into sectors. it has been observed that among the above-mentioned colonies the splitting of air mycelium into sectors of varied growth intensifies. the issued colonies grown from the spores from the sector of a feeble growth of the air mycelium are characterized by a considerable varia ...	1980	7424232
prevalence of precipitins in groups at risk of developing hypersensitivity pneumonitis.	a study was made of the prevalence of serum precipitins to micropolyspora faeni, thermoactinomyces vulgaris, aspergillus fumigatus, and pigeon serum in population groups suspected to be at high risk for the development of hypersensitivity pneumonitis. pigeon breeders' sera contained precipitins mainly to pigeon serum (38%) and a. fumigatus (18%). occupants of homes in which forced air heating systems were investigated for the presence of microorganisms reacted mostly with m. faeni (13%) and a. f ...	1980	6988108
[a new selection method for obtaining mutants of streptomyces glaucoachromogenes producing lambdamycin using the thermophilic phage-host system tal/thermoactinomyces vulgaris].	in order to obtain high-producing mutants of the lambdamycin producer streptomyces glaucoachromogenes imet 31 118, a new selection technique using the thermophilic phage-host system tal/thermoactinomyces vulgaris, was developed. lambdamycin is a yellow-green pigment antibiotic of the chromoglycoside type with antimicrobial, antiviral, cancerostatic, and ergotropic activity in vitro and in vivo. the physicochemical properties of lambdamycin resemble those of chartreusin. by means of the selection ...	1980	6990639
peripheral and alveolar response in guinea pigs to an aerosol exposure of thermoactinomyces candidus spores.		1980	6997733
[effect of storage of the thermotolerant actinomycete thermoactinomyces vulgaris on its enzymic activity].	various methods of storage (as soil cultures, in peptonecorn agar, and in the freeze-dried state) of the thermotolerant actinomycete thermoactinomyces vulgaris ra ii-4a, protease producer, were studied. the best method providing a high level of proteolytic activity was shown to be freeze-drying and subsequent selection of the most active variants. the optimal temperature for the storage of the freeze-dried culture was 4 degrees c. during a prolonged (12 months) storage of the actinomycete under ...	1980	7001434