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antibiotic material from bacillus licheniformis (weigmann, emend. gibson) active against species of mycobacteria. 194621066112
a simple medium for production of antibiotic by bacillus licheniformis. 194720247712
licheniformin, the antibiotic material from bacillus licheniformis; concentration and some chemical and biological properties. 194720343456
antibiotics produced by bacillus licheniformis; a practical chemically defined medium for production of licheniformin. 194918138166
antibiotic peptides from bacillus licheniformis; licheniformins a, b and c. 195213018122
the proteolytic system of bacillus licheniformis. 195513239633
[activation of botulinum d toxinogenesis by bacillus licheniformis]. 195913650308
antibiotic production as a function of spore formation in bacillus licheniformis. 195913799803
formation of bacitracin by washed cell suspensions of bacillus licheniformis. 196016561870
formation of bacitracin by protoplasts of bacillus licheniformis. 196116561908
stages in germination of spores of bacillus licheniformis.halmann, m. (the israel institute for biological research, ness ziona) and a. keynan. stages in germination of spores of bacillus licheniformis. j. bacteriol. 84:1187-1193. 1962.-this work defines conditions under which the first biochemical step in germination of spores (the so-called trigger reaction) could be studied separately from subsequent steps in germination. although the initiation of germination of spores of bacillus licheniformis occurred only above 20 c, and at ph 6, spores preincub ...196213952202
preliminary chemical characterization of the spore coats of bacillus licheniformis. 196213967670
the biosynthesis of d-amino acids in bacillus licheniformis. 196214460685
effects of metal ions on the optical specificity of glutamine synthetase and glutamyl transferase of bacillus licheniformis. 196214464064
thermophilic aerobacillus from banana puree.bacillus licheniformis was isolated from spoiled cans of banana purée. the isolate proved to be a thermophilic gas former which causes soft swell in cans of nonacidic food. thermal resistance of the spores was examined. heat treatment for at least 90 to 100 sec at 230 f was found necessary to assure commercial sterility of canned banana purée at ph 4.5.196214469756
transduction of bacillus licheniformis and bacillus subtilis by each of two phages.taylor, martha j. (u.s. army biological laboratories, fort detrick, frederick, md.) and curtis b. thorne. transduction of bacillus licheniformis and bacillus subtilis by each of two phages. j. bacteriol. 86:452-461. 1963.-a second transducing bacteriophage, designated sp-15, was isolated from the same soil-sample culture filtrate that supplied the bacillus subtilis transducing phage, sp-10, reported earlier from this laboratory. sp-10 and sp-15 differ serologically and in several other respects, ...196314066421
polyglutamic acid synthesis by cell-free extracts of bacillus licheniformis. 196314068536
transformation of bacillus licheniformis.gwinn, darrel d. (oregon state university, corvallis), and curtis b. thorne. transformation of bacillus licheniformis. j. bacteriol. 87:519-526. 1964.-when a series of 28 auxotrophic mutants of bacillus licheniformis were screened for transformation, only three of them, m28 (glycine(-)), m30 (uncharacterized), and m33 (purine(-)), produced a detectable number of transformants. the screening method consisted of spreading auxotrophic cells and deoxyribonucleic acid (dna) from the prototrophic stra ...196414127566
the distribution and formation of penicillinase in a bacterial population of bacillus licheniformis. 196414135542
biosynthesis of bacitracin. i. formation of bacitracin by a subcellular fraction of bacillus licheniformis. 196414166871
postlogarithmic phase metabolism of sporulating microorganisms. i. protease of bacillus licheniformis. 196414169155
transformation to prototrophy and polyglutamic acid synthesis in bacillus licheniformis.leonard, c. gomez (u.s. army biological laboratories, fort detrick, frederick, md.), d. k. mattheis, m. j. mattheis, and r. d. housewright. transformation to prototrophy and polyglutamic acid synthesis in bacillus licheniformis. j. bacteriol. 88:220-225. 1964.-growth conditions necessary for transformation to prototrophy of 14 auxotrophs of bacillus licheniformis were studied. the unexpected finding of different growth requirements by each auxotroph for the development of transformable cells is ...196414197892
[assay of streptomycin and its derivatives with bacillus licheniformis alter]. 196414337546
amino acid composition of bacillus licheniformis. 19645836558
role of amino acids in germination of bacillus licheniformis spores. ii. effect on radioactive spores. 19655843075
the isolation of structural components present in the cell wall of bacillus licheniformis n.c.t.c. 6346.1. four of the known components of wall preparations of vegative cells of bacillus licheniformis n.c.t.c. 6346 have been isolated free of each other after successive treatments of the walls with trichloroacetic acid and lysozyme: (a) a mucopeptide consisting of glucosamine, muramic acid, alphain-diaminopimelic acid, glutamic acid and alanine in the molar proportions 1.0:0.8:1.0:1.2:1.7; (b) an insoluble protein; (c) teichoic acid containing phosphorus and glucose in equimolar amounts; (d) teichu ...19655862410
the formation of penicillinase by cysteine-starved auxotrophs of bacillus licheniformis. 19655865512
the genetics of bacillus licheniformis penicillinase: a preliminary analysis from studies on mutation and inter-strain and intra-strain transformations. 19655883699
apparent induction of ornithine transcarbamylase and arginase by arginine in bacillus licheniformis. 196514338793
purification and properties of penicillinases from two strains of bacillus licheniformis: a chemical, physicochemical and physiological comparison.1. the penicillinases formed by penicillinase-constitutive mutant strains from two closely related varieties (749 and 6346) of bacillus licheniformis have been isolated, characterized and compared. they are chemically, physicochemically and immunologically very similar, but differ enzymologically in absolute and relative activity on, and affinity for, different penicillins and cephalosporins. 2. the molecular weights of both types are approx. 23000. neither enzyme contains any cyst(e)ine. howeve ...196514340057
protoplast lysis and inhibition of growth of bacillus licheniformis by bacitracin.snoke, john e. (university of california, los angeles), and neal cornell. protoplast lysis and inhibition of growth of bacillus licheniformis by bacitracin. j. bacteriol. 89:415-240. 1965.-the growth of bacillus licheniformis is inhibited by bacitracin, the antibiotic which the organism itself produces. the effect of the antibiotic is confined to the early growth phase of the organism. bacitracin will produce a rapid lysis of protoplasts of b. licheniformis and micrococcus lysodeikticus. the lyt ...196514255709
changes in the nucleotide poll of bacillus licheniformis during sporulation.leitzmann, claus (university of minnesota, minneapolis), and robert w. bernlohr. changes in the nucleotide pool of bacillus licheniformis during sporulation. j. bacteriol. 89:1506-1510. 1965.-an analysis of the amount of acid-soluble nucleotides in bacillus licheniformis cells showed a 75% increase during presporulation over that in log-phase cells. cultures in which presporulation was inhibited by actinomycin d showed a decrease in acid-soluble nucleotides during the same time interval. a separ ...196514291588
role of amino acids in germination of bacillus licheniformis spores. i. uptake of radioactive amino acids by germinating and dormant spores. 196514292212
different transforming characteristics of colonial variants from auxotrophic mutants of bacillus licheniformis. 196514329478
the cell wall of bacillus licheniformis n.c.t.c. 6346: biosynthesis of the teichuronic acid.1. particulate fractions prepared from disrupted cells of bacillus licheniformis n.c.t.c. 6346 catalyse the uptake of radioactivity from udp-[(14)c]glucuronic acid or udp-n[(14)c]-acetylglucosamine. maximal uptake requires the presence of both nucleotides and mg(2+) ions. the reaction is inhibited markedly by high concentrations of novobiocin and, to a certain extent, by vancomycin and by methicillin. 2. the radioactive product formed is resistant to pronase and is soluble in 5% (w/v) trichloroa ...196616742446
ornithine transcarbamylase enzymes: occurrence in bacillus licheniformis.two separate ornithine transcarbamylase enzymes have been found in extracts of late exponentialphase cells of bacillus licheniformis grown on glucose and l-arginine. one enzyme presumably has a biosynthetic function and is repressed by arginine. the other is induced by arginine, is relatively heat-stable, and can be separated from the first by diethylaminoethyl chromatography.19665910016
factors affecting transformation of bacillus licheniformis.thorne, curtis b. (fort detrick, frederick, md.), and harold b. stull. factors affecting transformation of bacillus licheniformis. j. bacteriol. 91:1012-1020. 1966.-transformation systems involving two types of transformable mutants of bacillus licheniformis 9945a were compared. each system required its specific growth medium, but a single transformation medium could be used for both. cells from a culture of optimal age were not competent, at least to any great extent, but they developed compete ...19665929742
catabolite repression of "three sporulation enzymes" during growth of bacillus licheniformis. 19665966398
multiple proteolytic enzymes of bacillus licheniformis. 19664959348
interspecies transformation between bacillus subtilis and bacillus licheniformis. 19664960110
protein synthesis by long-lived messenger ribonucleic acid in bacteria.1. experiments were performed to investigate two hypotheses about the function of long-lived messenger rna in bacteria. after rna synthesis had been stopped by the addition of actinomycin, continuing protein synthesis was used as a measure of persistent messenger rna. 2. the hypothesis that messenger rna responsible for the synthesis of membrane protein is exceptionally long-lived was tested in experiments with protoplasts of bacillus megaterium. however, this messenger rna proved to be of appro ...19664961300
antagonism between vegetative cells and l-forms of bacillus licheniformis strain 6346. 19664961308
the purification and properties of a penicillinase whose synthesis is mediated by an r-factor in escherichia coli.1. the penicillinase (beta-lactamase) from escherichia coli strain tem has been purified and its activity against a range of penicillin and cephalosporin derivatives measured. 2. the enzyme shows little resemblance to penicillinases from bacillus cereus, bacillus licheniformis and staphylococcus aureus. 3. the molecular weight of the enzyme is 16700+/-5%, which is about half the value obtained for other penicillinases. 4. the enzyme is most similar in properties to a crude preparation of a penic ...19665328167
clostridium botulinum type f: seasonal inhibition by bacillus licheniformis.clostridium botulinum type f has been identified during the summer months in mud samples from a small stream. its absence during the period from october to april in these mud samples is attributed to the presence of bacillus19675333132
changes in amino acid permeation during sporulation.changes in amino acid uptake in bacillus licheniformis and in the amino acid pools of three bacillus species were investigated, by use of cells from different stages of the life cycle. b. licheniformis contains catalytic uptake systems for all of the 10 amino acids studied. the apparent maximal velocities of uptake decreased during sporulation but did not fall below the range observed for other microorganisms. in sporulating cells, the apparent affinity constants of the uptake systems for indivi ...19676025411
regulation of glutamine synthetase. ii. patterns of feedback inhibition in microorganisms.the feedback inhibition of glutamine synthetase was investigated by use of partially purified enzyme preparations from salmonella typhimurium, micrococcus sodonensis, pseudomonas fluorescens, bacillus cereus, bacillus licheniformis, clostridium pasteurianum, rhodospirillum rubrum, neurospora crassa, candida utilis, and chlorella pyrenoidosa. inhibition analyses indicated that the enzyme of each organism can be effectively regulated with mixtures of end products from the diverse pathways of gluta ...19676025412
energetics and motility in bacillus licheniformis. 19676025456
cell-bound penicillinase of bacillus licheniformis; properties and purification. 19676038893
release of penicillinase by bacillus licheniformis. 19676038894
regulation of glutamine synthetase. v. partial purification and properties of glutamine synthetase from bacillus licheniformis.the glutamine synthetase of bacillus licheniformis has been obtained at about 15% purity. sucrose gradient centrifugation gave a molecular weight value of approximately 612,000. both l- and d-glutamate can be utilized as substrates in the biosynthetic reaction, although the l isomer was five times more active. the requirement for adenosine triphosphate (atp) can be partially replaced by guanosine or inosine triphosphates, but not by cytidine or uridine triphosphates. the mn(++) was required for ...19676051339
regulation of glutamine synthetase. vi. interactions of inhibitors for bacillus licheniformis glutamine synthetase.the relationships of five feedback inhibitors for the bacillus licheniformis glutamine synthetase were investigated. the inhibitors were distinguishable by differences in their competitive relationship for the substrates of the enzyme. mixtures of l-glutamine and adenosine-5'-monophosphate (amp) or histidine and amp caused synergistic inhibition of glutamine synthesis. histidine, alanine, and glycine acted antagonistically toward the l-glutamine inhibition. alanine acted antagonistically toward ...19676051340
control of aspartokinase during development of bacillus licheniformis. 19676066293
threonine dehydratase of bacillus licheniformis. i. purification and properties. 19685636377
the isolation and characterisation of cytoplasmic membranes and mesosomes of bacillus licheniformis 6346. 19685644123
isolation and characterization of the disaccharide n-acetyl-glucosaminyl-beta(1 bound to 4)-n-acetylmuramic acid and two tripeptide derivatives of this disaccharide from lysozyme digests of bacillus licheniformis atcc 9945 cell walls. 19685648432
sequential replication of the chromosome of bacillus licheniformis.a sequential replication map of the chromosome of bacillus licheniformis was constructed by employing the method of gene-frequency analysis presented by yoshikawa and sueoka. our analysis of 11 genetic markers was based on the hypothesis that the chromosome initiated replication at a fixed origin and proceeded in a linear fashion to the terminus. the proposed locations of markers were validated by cotransformation and cotransduction analyses. bacteriophage sp-15 cotransduced markers that failed ...19685669889
regulation of arginine and proline catabolism in bacillus licheniformis.the enzymes in the arginine breakdown pathway (arginase, ornithine-delta-transaminase, and delta'-pyrroline-5-carboxylate dehydrogenase) were found to be present in bacillus licheniformis cells during exponential growth on glutamate. these enzymes could be coincidentally induced by arginine or ornithine to a very high level and their synthesis could be repressed by the addition of glucose, clearly demonstrating catabolite repression control of the arginine degradative pathway. the strongest cata ...19685674049
biosynthesis of bacitracin. ii. incorporation of 14c-labeled amino acids into bacitracin by a cell-free preparation from bacillus licheniformis. 19685680606
characteristics of penicillinase release by washed cells of bacillus licheniformis.saline-washed cells of bacillus licheniformis strain 749/c (constitutive for penicillinase) were able to release exopenicillinase in the presence of concentrations of chloramphenicol that prevented protein synthesis completely. the release reaction was strongly ph-dependent, occurring at a faster rate at alkaline ph in anionic or cationic buffers than at neutral ph. a strongly ph-dependent release reaction was noted in growing cells also. the reaction in washed cells can be stopped completely by ...19685685998
morphological phenomena associated with penicillinase induction and secretion in bacillus licheniformis.cells of uninduced bacillus licheniformis (strain 749) in mid-logarithmic phase have no extensive intracytoplasmic membrane. after induction with cephalosporin c, characteristic organelles that contain tubules and vesicles with single-layered membranes and no visible internal substructure can be seen in thin sections in the periplasm. a magnoconstitutive penicillinase producer (749/c) contains similar structures. it is suggested that they represent a penicillinase secretory apparatus. in the fir ...19685686003
threonine dehydratase of bacillus licheniformis. ii. regulation during development. 19685688827
[fusidic acid evaluation using bacillus licheniformis]. 19685719704
the cell wall of bacillus licheniformis n.c.t.c. 6346. composition of the mucopeptide component.1. the mucopeptide component of wall preparations from bacillus licheniformis was obtained in soluble form by treatment of the acid-insoluble residue of walls with lysozyme. 2. the soluble mucopeptide contains glutamic acid, diaminopimelic acid, alanine, n-acetylglucosamine and n-acetylmuramic acid in the molecular proportions 1.0:1.0:1.6:0.8:0.7. in addition approx. 1 mole of amide/mole of glutamic acid is present. essentially all of the dry weight and nitrogen content of soluble mucopeptide is ...19685721470
the cell wall of bacillus licheniformis n.c.t.c. 6346. isolation of low-molecular-weight fragments from the soluble mucopeptide.1. soluble mucopeptide was prepared by lysozyme treatment of acid-extracted walls of bacillus licheniformis n.c.t.c. 6346 and separated into fractions differing in molecular size by chromatography on sephadex g-25 and g-50. 2. about 16% of the weight of soluble mucopeptide has a weight-average molecular weight in excess of 20000. about one half has a weight-average molecular weight of less than 2000 and the balance of soluble mucopeptide is of intermediate size. 3. in the mucopeptide fractions i ...19685721471
the regulation and kinetics of the two ornithine transcarbamylase enzymes of bacillus licheniformis. 19685725163
[effect of chloramphenicol on the synthesis of bacitracin in bacillus licheniformis]. 19685751196
lifetime of messenger ribonucleic acid for penicillinase synthesis in several strains of bacilli.1. previous studies of penicillinase synthesis in bacillus licheniformis showed that enzyme synthesis after the addition of actinomycin continues for far longer in the constitutive mutant 749/c than in the parental inducible strain (yudkin, 1966). this result was interpreted as indicating a difference in the lifetime of specific messenger rna in the two strains. other bacilli have now been examined in an attempt to see whether this difference is general. 2. there was no difference in the lifetim ...19684969990
synthesis of teichoic acids. vii. synthesis of teichoic acids during spore germination.the synthesis of teichoic acids has been examined during germination in bacillus licheniformis atcc 9945 and in b. subtilis w-23. teichoic acids are absent from the spores of both organisms. b. licheniformis spores lack the enzymes responsible for teichoic acid synthesis. the appearance of these enzymes during germination is correlated with the appearance of teichoic acids in the cell. the appearance of teichoic acid-synthesizing enzymes and of teichoic acids in the cell are inhibited by the add ...19684970221
cell wall or membrane mutants of bacillus subtilis and bacillus licheniformis with grossly deformed morphology. 19684970364
characteristics of secretion of penicillinase, alkaline phosphatase, and nuclease by bacillus species.the distribution of alkaline phosphatase and nuclease activity between cells and medium was examined in one strain of bacillus licheniformis and four strains of b. subtilis. over 95% of both activities was found in the medium of the b. licheniformis culture, but in the b. subtilis cultures the amount of enzyme activity found in the medium varied with the strain and the enzyme considered. b. licheniformis 749 and its penicillinase magnoconstitutive mutant 749/c were grown in continuous culture wi ...19684970649
diaminopimelate decarboxylase of sporulating bacteria.the meso-diaminopimelate (dap) decarboxylase of bacillus licheniformis, a pyridoxal phosphate-requiring enzyme, was stabilized in vitro by 0.15 m sodium phosphate buffer (ph 7.0) containing 1 mm 2,3-dimercaptopropan-1-ol, 100 mug of pyridoxal phosphate per ml, and 3 mm dap. when the meso-dap concentration was varied, the enzyme in cell-free extracts of b. licheniformis exhibited michaelis-menten kinetics. pyridoxal phosphate was the only pyridoxine derivative which acted as a cofactor. the enzym ...19684972917
localization of cell-bound penicillinase in bacillus licheniformis.when protoplasts are prepared from bacillus licheniformis (strain 749/c, constitutive for penicillinase), approximately 60% of the cell-bound penicillinase is released. the remainder is retained by the protoplast and cannot be removed by washing. this release is specific, in that less than 7% of the cellular reduced nicotinamide adenine dinucleotide (nadh) dehydrogenase and alpha-glucosidase is liberated by the treatment. the freed penicillinase is excluded from g-200 sephadex, and it is partial ...19684302175
some enzymic activities and chemical properties of the mesosomes and cytoplasmic membranes of bacillus licheniformis 6346.1. the liberation of the vesicles of the mesosomes from protoplasts of bacillus licheniformis strain 6346 is controlled by the mg(2+) concentration present during the removal of the walls by lysozyme. 2. the functioning of the cytoplasmic membranes is also critically controlled by the mg(2+) concentration. 3. the isolated mesosomes and the cytoplasmic membranes differ in their enzymic activities. both succinate dehydrogenase and nadh oxidase activities are very low or absent from the mesosomes. ...19694308839
regulated enzymes of aromatic amino acid synthesis: control, isozymic nature, and aggregation in bacillus subtilis and bacillus licheniformis.several regulated enzymes involved in aromatic amino acid synthesis were studied in bacillus subtilis and b. licheniformis with reference to organization and control mechanisms. b. subtilis has been previously shown (23) to have a single 3-deoxy-d-arabinoheptulosonate 7-phosphate (dahp) synthetase but to have two isozymic forms of both chorismate mutase and shikimate kinase. extracts of b. licheniformis chromatographed on diethylaminoethyl (deae) cellulose indicated a single dahp synthetase and ...19694977689
cotransduction and cotransformation of genetic markers in bacillus subtilis and bacillus licheniformis.bacteriophage sp-15, a large generalized transducing phage of bacillus, was compared with phages pbs-1 and sp-10 for the ability to cotransduce pairs of genetic markers exhibiting different degrees of linkage. when auxotrophs of b. subtilis w-23 were used as recipients, sp-15 and pbs-1 effected a much higher frequency of cotransduction than did sp-10 with markers that were not closely linked. with more closely linked loci, the differences were not as great. sp-15 cotransduced linked markers at a ...19694982666
the amino acid sequence of penicillinase from bacillus licheniformis. 19695353499
regulations of penicillinase synthesis in bacillus licheniformis: binding of inducer to cells, and the half-life of the messenger ribonucleic acid. 19695360696
the stability and cell content of penicillinase messenger rna in bacillus licheniformis. 19695370294
extracellular cationic proteases of bacillus licheniformis. 19695374021
the regulation of aspartokinase in bacillus licheniformis. 19695772406
characteristics of penicillinase secretion by growing cells and protoplasts of bacillus licheniformis.cultures of the inducible penicillinase-producing strain 749 of bacillus licheniformis, induced with small amounts of benzylpenicillin, synthesized penicillinase at a high rate for a short period, after which the rate of synthesis slowly declined. during the period of active synthesis, the rate of secretion, as a fraction of the level of cell-bound penicillinase (which is originally high), gradually decreased to a constant level. chloramphenicol, at a concentration (40 mug/ml) which completely i ...19695773030
an electron microscopic study of cell growth and mesosomal structure of bacillus licheniformis. 19695775370
protein synthesis by cell-free extracts from bacillus licheniformis. 19695776191
variable gene linkage and the phased appearance of transformants in bacillus licheniformis. 19695783898
cell-free amino acid-incorporating systems from bacillus licheniformis and bacillus stearothermophilus 10.cell-free amino acid-incorporating systems were prepared from log-phase cells of bacillus licheniformis and b. stearothermophilus 10. no differences were observed between the two systems with respect to the types and relative amounts of the ribosomes, the time dependence of incorporation, and the optimal magnesium ion concentration (4 mm). the b. licheniformis system showed greater dependence on added transfer ribonucleic acid (trna) and was less stimulated by polyuridylic acid (poly u) than the ...19695788703
conditions for competence in the bacillus licheniformis transformation system.some variables in the bacillus licheniformis transformation system, such as inoculum size, initial cell density in transformation medium, and time of optimal competence, have been explored, and a methodology has been developed to obtain greater reproducibility and maximal competence for transformation. the transformation system in b. licheniformis has been shown to be similar to that of b. subtilis in a number of respects, including "dormancy" of transformed cells and the concentration of transf ...19695802618
dihydrodipicolinic acid synthase of bacillus licheniformis. 19694901235
periplasmic structure of frozen-etched and negatively stained cells of bacillus licheniformis as correlated with penicillinase formation.bacillus licheniformis strain 749/c (constitutive for penicillinase formation) and uninduced cells of strain 749 (penicillinase-inducible) were examined after freezeetching. in the early stationary phase, strain 749/c organisms had clusters of vesicles (30 to 40 nm in diameter) on the outer surface of the plasma membrane. these are randomly distributed on the membrane, including the region of septum formation. the vesicles are not intimately associated with the plasma membrane, and their inner a ...19694187700
modification of the appearance of mesosomes in sections of bacillus licheniformis according to the fixation procedures. 19704906926
a mechanism for penicillinasesecretion in bacillus licheniformis.cell-bound isozymes of penicillinase are distinguished from extracellular enzyme by their capacity to bind deoxycholate and to elute with an apparent molecular weight of 45,000 on gel filtration in its presence. by methods that are unlikely to involve changes in primary structure, the cell-bound forms (both from the plasma membrane and from the periplasmic vesicles) can be converted to forms that are very similar if not identical to the exo-form (i.e., eluting with a molecular weight of 24,000 i ...19705266165
the preparation of iodinated vancomycin and its distribution in bacteria treated with the antibiotic.vancomycin was radioactively labelled by iodination with (125)i.iodinated vancomycin was only a little less potent as an antibiotic than vancomycin itself. it was shown, both by chromatography and differential absorption measurements, to combine with acyl-d-alanyl-d-alanine residues. radioactive vancomycin was used to follow the fate of the antibiotic in bacteria that had been subjected to the least concentration required to inhibit growth. most of the radioactivity was in the cell walls, althou ...19705411430
the cell wall of bacillus licheniformis n.c.t.c. 6346. linkage between the teichuronic acid and mucopeptide components.1. after extraction of teichoic acid from cell walls of bacillus licheniformis with dilute alkali, the insoluble residue contains the teichuronic acid and mucopeptide components and a small amount of residual phosphorus. 2. a complex of teichuronic acid and a part of the mucopeptide was isolated from the soluble fraction obtained by lysozyme treatment of alkali extracted walls. 3. small-molecular-weight mucopeptide fragments, not containing teichuronic acid, are obtained from the soluble fractio ...19705419740
some structural features of the teichuronic acid of bacillus licheniformis n.c.t.c. 6346 cell walls.a teichuronic acid, containing glucuronic acid and n-acetylgalactosamine, was purified from acid extracts of bacillus licheniformis 6346 cell walls as described by janczura, perkins & rogers (1961). after reduction of the carboxyl function of glucuronic acid residues in the polysaccharide the reduced polymer contains equimolar amounts of n-acetylgalactosamine and glucose. methylation of the reduced polysaccharide by the hakamori (1964) technique showed the glucose residues to be substituted on c ...19705419741
the distribution of polysomes, ribosomes and ribosomal subunits in exponential-phase cells of bacillus licheniformis. 19705440625
an electron microscopic study of the structure of mesosomal membranes in bacillus licheniformis. 19705445835
the induction of bacillus licheniformis penicillinase by vanadate, molybdate and tungstate anions. 19705473204
phospholipid metabolism during penicillinase production in bacillus licheniformis.during membrane-bound penicillinase production, bacillus licheniformis forms vesicles and tubules that do not appear in the absence of penicillinase production. the major lipids of b. licheniformis were shown to be phospholipids. the proportions, metabolism, and the total phospholipid per cell were shown to be essentially the same in the uninduced control, induced and constitutive penicillinase forming cells during both the exponential and stationary growth phases. membrane phospholipids were no ...19705473893
penicillinase (beta-lactamase) induction in bacillus licheniformis under pseudogratuitous conditions by 2-(2'-carboxyphenyl)-benzoyl-6-aminopenicillanic acid.induction of penicillinase (beta-lactamase) in bacillus licheniformis 749 by 2-(2'-carboxyphenyl)-benzoyl-6-aminopenicillanic acid (cbap) was examined, since this compound was reported to be a gratuitous inducer of penicillinase in staphylococcus aureus. the specific activity of enzyme optimally induced by cbap is slightly more than that formed in response to cephalosporin c and threefold the level induced by benzylpenicillin. the optimal inducer concentration of cbap was not inhibitory toward t ...19705473897
membrane-bound penicillinase of bacillus licheniformis 749-c. 19705484656
formation and life cycle of ribosomal subunits, mono- and polyribosomes in bacillus licheniformis. 19705497143
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