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recombinant lactococcus lactis fails to secrete bovine chymosine.bovine chymosin is an important milk-clotting agent used in the manufacturing of cheeses. currently, the production of recombinant proteins by genetically modified organisms is widespread, leading to greatly reduced costs. lactococcus (l.) lactis, the model lactic acid bacterium, was considered a good candidate for heterologous chymosin production for the following reasons: (1) it is considered to be a gras (generally regarded as safe) microorganism, (2) only one protease is present on its surfa ...025482140
the inhibiting effect of streptococcus lactis on lactobacillus bulgaricus. 192816559344
concerning the habitat of streptococcus lactis. 193516559869
factors influencing the rate of fermentation of streptococcus lactis. 193816560127
nutritional studies on streptococcus lactis: i. an unidentified growth factor found in yeast extract. 194316560713
a new growth factor for streptococcus lactis. 194317789876
the serological grouping of streptococcus lactis (group n) and its relationship to streptococcus faecalis. 194320475672
relationship of a new growth factor required by certain hemolytic streptococci to growth phenomena in other bacteria.by a series of quantitative assays of concentrates prepared in a variety of ways, it has been shown that the previously described growth factor now called "strepogenin," required by certain hemolytic streptococci, is very similar to and possibly identical with the recently described growth factors for lactobacillus casei and smith's streptococcus lactis. an improved procedure for assay of strepogenin with the aid of lactobacillus casei has been described, and additional properties of the growth ...194419871409
relation of the streptococcus lactis r factor to "folic acid". 194417796469
nutrition of streptococcus lactis. 194416560784
the identification of "streptococcus lactis r" as a strain of streptococcus faecalis. 194416560875
transformation of the streptococcus lactis r factor to "folic acid" by resting cell suspensions of enterococci. 194516560991
isolation from liver of new factors essential for the growth of lactobacillus helveticus and streptococcus lactis r. 194516747916
isolation from liver of new factors essential for the growth of lactobacillus helveticus and streptococcus lactis r. 194521009595
transformation of the streptococcus lactis r factor to folic acid by resting cell suspensions of enterococci. 194520989339
the growth response of tetrahymena geleii w to folic acid and to the streptococcus lactis r factor. 194620994559
the differentiation of streptococcus cremoris and streptococcus lactis by means of bacteriophage action. 194621013069
the growth response of tetrahymena geleii w to folic acid and to the streptococcus lactis r factor. 194617732388
the differentiation of streptococcus cremoris and streptococcus lactis by means of bacteriophage action. 194620475736
the folic acid group of growth factors; the complexity of growth factors for streptococcus lactis r. 194720247696
the folic acid group of growth factors; the effect of folic acid on acid production by growing cultures of streptococcus lactis r. 194720247697
isolation of rhizopterin, a new growth factor for streptococcus lactis r. 194720270825
the folic acid group of growth factors: 1. the complexity of growth factors for streptococcus lactis r. 194716748157
the folic acid group of growth factors: 2. the effect of folic acid on acid production by growing cultures of streptococcus lactis r. 194716748158
electron microscope studies of bacteriophage active against streptococcus lactis. 194916561709
electron microscope studies of bacteriophage active against streptococcus lactis. 194918120602
the streptococcus lactis host-virus system; factors influencing quantitative measurement of the virus. 194915393691
the streptococcus lactis host-virus system; characteristics of virus growth and the effect of electrolytes on virus adsorption. 194915393692
[bacteriophage in the production of cheese; resistance of streptococcus lactis to cheese bacteriophage]. 195015438750
the relation of calcium and other constituents of a defined medium to proliferation of lactic streptococcus bacteriophage. 195014794619
[improvement of pasteurized milk through living streptococcus vaccine]. 195114823534
influence of ph on proliferation of lactic streptococcus bacteriophage. 195114824082
growth and nisin production of a strain of streptococcus lactis. 195114824485
a new bacteriophage active against a lactic streptococcus. 195114832208
[variability of lactic streptococci; plicated forms of streptococcus lactis and streptococcus diacetilactis]. 195114842791
various antibiotics from one strain of streptococcus lactis. 195114843161
[metabolism of vitamin factors during bacterial growth; research on streptococcus lactis r, lactobacillus arabinosus and lactobacillus casei]. 195114847850
[bacteriologic investigation of certain derivatives of pteroylglutamic acid]. 195114857682
[the effect of 4-aminopterin on lactobacillus arabinosus and streptococcus lactis: appearance of resistent variants and modification of their synthetic capacities]. 195213017911
conversion of citrulline to ornithine by cell-free extracts of streptococcus lactis. 195313092957
[secondary phago-resistant cultures of streptococcus lactis]. 195313132539
[production of phagoresistant strains of streptococcus lactis with valuable properties in industry]. 195313144385
stoicheiometry of the citrulline phosphorylase reaction of streptococcus lactis. 195413172192
streptococcus lactis isolated from a patient with subacute bacterial endocarditis. 195513228455
the effect of methionine and methionine precursors on the growth of streptococcus lactis. 195513233161
[seasonal variations of lactic fermentation by str. lactis in milk. ii. vitality of streptococcus lactis in milk in various seasons]. 195513430384
the involvement of phosphorylated serine in the conversion of homocysteine to methionine by streptococcus lactis. 195514367306
[effect of streptococcus lactis and of its filtrates on the development of streptococcus paracitrovorus in milk]. 195613321529
[utilization of phosphoric acid by streptococcus lactis during its growth on milk culture]. 195613386974
[studies on lactase activity of streptococcus lactis]. 195613386975
[antagonism between streptococcus lactis, bacillus subtilis and pseudomonas fluorescens in milk]. 195613394260
observations on the methionine nutrition of streptococcus lactis. 195713460824
[examination of activity lactase of streptococcus lactis. ii]. 195713464455
factors affecting the initiation of respiration of streptococcus lactis. 195713470850
[nisin, an antibacterial substance from streptococcus lactis with a reference to the resistance problem]. 195713470982
[acetoin and diacetyl in metabolism of streptococcus lactis]. 195713483121
[activation of lactic processes by streptococcus lactis in milk]. 195713526574
[studies on maximum production of streptococcus lactis cells cultured on whey]. 195713544902
[synthesis of phosphoric esters by streptococcus lactis]. 195813643749
ornithine-citrulline enzyme synthesis in biotin-deficient cells of streptococcus lactis. 195813539014
properties and biotin content of purified preparations of the ornithinecitrulline enzyme of streptococcus lactis. 195913654396
[vitamin requirement and synthesis of certain vitamin b groups in streptococcus lactis cultures]. 195913837625
[stimulation of phagolysis of streptococcus lactis by various agents]. 195914420894
sparing effect on the folic acid requirement of streptococcus lactis by 5-amino-2, 4-bis(substituted-amino) pyrimidines. 196014412761
[a new variety of streptococcus lactis]. 196013832553
a study of carbamyl phosphate synthesis in streptococcus lactis. 196113739864
uptake of strontium and calcium by streptococcus lactis. 196213885362
quantity and fatty acid composition of lipid extracted from cells of streptococcus lactis.macleod, patricia (university of connecticut, storrs), r. g. jensen, g. w. gander, and j. sampugna. quantity and fatty acid composition of lipid extracted from cells of streptococcus lactis. j. bacteriol. 83:806-810. 1962.-a method for the extraction of lipid from bacterial cells is described. lysozyme-treated cells of streptococcus lactis yielded 5% of their dry weight as lipid, whereas cells not treated with lysozyme yielded only 3% lipid. more than two-thirds of the bacterial lipid extracted ...196214468026
effect of bromidehypochlorite bactericides on microorganisms.a new principle in compounding stable, granular bactericidal products led to unique combinations of a water-soluble inorganic bromide salt with a hypochlorite-type disinfectant of either inorganic or organic type. microbiological results are shown for an inorganic bactericide composed of chlorinated trisodium phosphate containing 3.1% "available chlorine" and 2% potassium bromide, and for an organic bactericide formulated from sodium dichloroisocyanurate so as to contain 13.4% "available chlorin ...196213977149
identification of stimulatory factor involved in symbiotic growth of streptococcus lactis and streptococcus cremoris.dahiya, r. s. (north carolina state college, raleigh) and m. l. speck. identification of stimulatory factor involved in symbiotic growth of streptococcus lactis and streptococcus cremoris. j. bacteriol. 85:585-589. 1963.-single-strain cultures of streptococcus lactis and s. cremoris isolated from a commercial starter culture showed symbiotic growth in milk. this study dealt with identification of the main component responsible for high acid production resulting from the combined growth of these ...196314042935
identification of growth stimulants for streptococcus lactis.koburger, j. a. (north carolina state college, raleigh), m. l. speck, and l. w. aurand. identification of growth stimulants for streptococcus lactis. j. bacteriol. 85:1051-1055. 1963-the growth of streptococcus lactis in milk was accelerated by pancreas extract, and three distinct components that were detected by bioautography were isolated and identified. these were purified by precipitation with silver ions, and their silver salts were regenerated in acidic solution. after adsorption and eluti ...196314043994
fatty acid composition of lipids from streptococcus cremoris and streptococcus lactis var. maltigenes.macleod, patricia (university of connecticut, storrs) and joseph p. brown. fatty acid composition of lipids from streptococcus cremoris and streptococcus lactis var. maltigenes. j. bacteriol. 85:1056-1060. 1963.-a method for the extraction of lipids from bacterial cells, involving grinding in a ball mill, is described. cells of streptococcus lactis var. maltigenes and s. cremoris yielded 5.3 and 5.5%, respectively, of their dry cellular weights as lipid. the lipids of both organisms were separat ...196314043995
free radical formation and survival of lyophilized microorganisms.heckly, robert j. (university of california, berkeley), r. l. dimmick, and j. j. windle. free radical formation and survival of lyophilized microorganisms. j. bacteriol. 85:961-966. 1963.-a correlation between death and spontaneous free radical production, measured by an increase in the relative electron paramagnetic resonance (epr) signal, was shown to exist for several species of microorganisms stored in the freezedried state, but the relationship between the free radical concentration and num ...196314044024
some factors influencing acid production by an oxytetracycline-resistant strain of streptococcus lactis.induction of oxytetracycline resistance in a strain of streptococcus lactis caused this organism to display reduced acid production, salt tolerance, pyruvate synthesis, growth at alkaline ph, and a loss in ability to produce ammonia from arginine. alpha-ketoglutaric and oxaloacetic acids were found to accumulate in the growth medium of resistant cells, in contrast to none in the medium of susceptible cells. no free arginine could be detected in the intracellular fraction of resistant cells, but ...196314063784
injury and death of streptococcus lactis due to freezing and frozen storage.cells of streptococcus lactis were harvested in the early stationary phase, washed, and resuspended in either skim milk (10% nonfat milk) or buffered distilled water (0.0003 m dipotassium phosphate, ph 7.2). samples of each suspension were frozen and stored at -20 c for intervals up to 28 days. colony counts of the frozen culture were made using lactic agar and a "restricted" lactic agar medium (tryptone reduced to 0.5%) to determine injury and death. death was determined by the difference in pl ...196313936199
[the transgalactosylase activity of streptococcus lactis]. 196313939143
differentiation of streptococcus lactis var. maltigenes from other lactic streptococci.strains of lactic streptococci isolated from samples of raw milk which had developed a malty aroma were subjected to the cultural, physiological, and serological tests commonly employed in the classification of streptococci. none of the strains could be differentiated from streptococcus lactis by these tests. resting cells of strains which produced an organoleptically detectable malty aroma when cultured in milk were usually found to possess an active alpha-ketoacid decarboxylase, indicating the ...196313949187
extracellular proteinase of streptococcus lactis.williamson, w. t. (north carolina state of the university of north carolina, raleigh), s. b. tove, and m. l. speck. extracellular proteinase of streptococcus lactis. j. bacteriol. 87:49-53. 1964.-streptococcus lactis was shown to produce an extracellular proteolytic enzyme(s). a 120-fold purification of the proteinase was obtained from the cell-free culture medium by ammonium sulfate fractionation, calcium phosphate gel treatment, and chromatography on diethylaminoethyl cellulose. only 32% of th ...196414102873
studies on the streptococcus lactis folate activity of human liver tissue. 196414133896
phospholipids of streptococcus lactis. 196414188741
[variability of antagonistic properties of streptococcus lactis under the influence of ultraviolet rays and some mutagenic substances]. 196414301107
effects of lactic acid-forming bacteria on vibrio comma inoculated into intestinal segments of rabbits.hattori, h. (sankyo co., ltd., tokyo, japan), h. misawa, i. igarashi, and y. sugiya. effects of lactic acid-forming bacteria on vibrio comma inoculated into intestinal segments of rabbits. j. bacteriol. 90:541-545. 1965.-mixed inocula of vibrio comma kc-4 and various lactic acid-forming bacteria were injected into the intestinal segments of rabbits (de and chatterje, 1953) to observe the effects of the latter agents in altering the changes produced by strain kc-4. the animals were sacrificed 10 ...196514329471
beta-galactosidase of streptococcus lactis.citti, j. e. (oregon state university, corvallis), w. e. sandine, and p. r. elliker. beta-galactosidase of streptococcus lactis. j. bacteriol. 89:937-942. 1965.-synthesis of beta-galactosidase by several strains of streptococcus lactis was induced by lactose. the rate of hydrolysis of o-nitrophenyl-beta-d-galactopyranoside was used to measure enzyme activity. the enzyme of all but one strain was unstable when whole cells were sonic-treated or treated with toluene; the enzyme of one strain of s. ...196514276118
comparison of slow and fast acid-producing streptococcus lactis. 196514285562
oxytetracycline-binding properties of streptococcus lactis. 19655837886
damage to streptococcus lactis resulting from growth at low ph.harvey, r. j. (the dairy research institute, palmerston north, new zealand). damage to streptococcus lactis resulting from growth at low ph. j. bacteriol. 90:1330-1336. 1965.-growth of streptococcus lactis below ph 5.0 results in damage to the cells. a period of growth below ph 5.0 is required before this damage is expressed, and a period of growth above ph 5.0 is required to correct the damage. part of the damage consists of a reduction in the specific activities of a number of enzymes. at the ...19655848331
[isolation in pure form and study of the products of transgalactozlation of streptococcus lactis]. 19655869324
[role of hydrolytic and transgalactosilase processes in the course of lactic acid fermentation under the influence of streptococcus lactis]. 19655869339
[effect of the environmental conditions on some aspects of carbohydrate metabolism in streptococcus lactis]. 19655871941
antibiotic influence on arginine desimidase activity by streptococcus lactis. 19655898727
identity of additional aroma constituents in milk cultures of streptococcus lactis var. maltigenes. 19665952149
effects of additives on the survival of lactic streptococci in frozen storage.three single-strain cultures, streptococcus lactis c(2), s. cremoris r(1), and s. diacetilactis drc(2), were frozen and stored in skim milk, in skim milk containing apple juice, and in skim milk containing one of the following additives: glycerol (10%, v/v), dimethyl sulfoxide (10%, v/v), l-malic acid (0.5 and 2.0%, w/v), acetamide (0.5 and 2.0%, w/v), or succinimide (0.5 and 2.0%, w/v). cultures were frozen and stored at -23.3 c, frozen and stored at -196 c in liquid nitrogen, or frozen at -196 ...19665953603
temperature-dependent association-dissociation of streptococcus lactis intracellular proteinase. 19665962492
long-term storage of bacteriophages of lactic streptococci.four phage strains representing phages of streptococcus lactis, s. cremoris, and s. diacetilactis were selected for the observation of the effect of cold storage on their viability. phages were stored at 4 c and at -18 c, or were frozen at approximately -70 c and stored at -18 c. they were found to display a high degree of stability with these storage methods. the same phage strains showed good stability to storage at room temperature for 3 weeks after thawing and also to alternate freezing and ...19665970828
[endocarditis lenta caused by streptococcus lactis]. 19665975414
[vanadium and experimental caries. vii. action of vanadium on the development of lactobacillus acidophilus and streptococcus lactis]. 19665225407
purification and properties of streptococcus lactis beta-galactosidase.beta-galactosidase of streptococcus lactis 7962 was partially purified, and its properties were studied. enzyme from only this strain of numerous lactic streptococci tested was stable in cell exudates prepared by various means. cell-free extracts of the 7962 strain were prepared by sonic treatment of washed cells previously grown in presence of lactose to fully induce enzyme synthesis. protamine sulfate precipitation of the nucleic acids and ammonium sulfate precipitation of protein were used fo ...19676025431
lactose and maltose uptake by streptococcus lactis. 19676038504
decarboxylation of alpha-keto acids by streptococcus lactis var. maltigenes.decarboxylation rates for a series of c-3 to c-6 alpha-keto acids were determined in the presence of resting cells and cell-free extracts of streptococcus lactis var. maltigenes. the c-5 and c-6 acids branched at the penultimate carbon atom were converted most rapidly to the respective aldehydes in the manner described for alpha-carboxylases. pyruvate and alpha-ketobutyrate did not behave as alpha-carboxylase substrates, in that o(2) was absorbed when they were reacted with resting cells. the sa ...19676049293
function of nisin and nisin-like basic proteins in the growth cycle of streptococcus lactis. 19676066110
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