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prophylactic immunization against streptococcus sanguis. 194816561586
prophylactic immunization against streptococcus sanguis. 194818887816
serologic relationship between streptococcus group h and streptococcus sanguis. 194918149442
[streptococcus sanguis white in subacute bacterial endocarditis]. 195015441351
hydrolysis of proteins and nucleic acids by lancefield group a and other streptococci.deibel, r. h. (american meat institute foundation, chicago, ill.). hydrolysis of proteins and nucleic acids by lancefield group a and other streptococci. j. bacteriol. 86:1270-1274. 1963.-classically, group a streptococci have been considered to be nonproteolytic in spite of the observation that a proteinase is produced in broth cultures. when tested by the plate method, it was observed that 78% of 47 strains were proteolytic when the cultures were incubated anaerobically. some strains hydrolyze ...196314086100
zooglea-forming streptococci, resembling streptococcus sanguis, isolated from dental plaque in man. 19655216256
inhibition of streptococcal dextransucrase by sera of rabbits infected with streptococcus sanguis. 19685244310
prevalence of streptococcus sanguis and streptococcus mutans in the mouth of persons wearing full-dentures. 19695255438
reaction rate of dextransucrase from streptococcus sanguis in the presence of various compounds. 19695255458
purification and properties of dextransucrase from streptococcus sanguis. 19695255459
distribution and serological specificity of sialidase produced by various groups of streptococci.the occurrence of a streptococcal sialidase (designated st-sialidase) in culture fluids of various streptococci was investigated. st-sialidase was found to occur in strains belonging to groups a, b, c, e, g, h, and l, and the unclassified strains, streptococcus sanguis and streptococcus uberis. st-sialidase of group a was confined predominantly to types 4 and 22. st-sialidases, extracted from the culture fluids of some selected strains, were antigenic, eliciting the formation of antibody which e ...19695344099
predominant catalase-negative soil bacteria. i. streptococcal population indigenous to soil.streptococci related to streptococcus sanguis were shown to represent a major segment of the bacterial flora of certain soils. upon initial isolation, these streptococci were quite pleomorphic and displayed morphological characteristics common to several other genera of bacteria, but after prolonged cultivation on laboratory media the isolates demonstrated a typical streptococcal morphology. it was proposed that a requirement for correction of the control mechanisms affecting growth rate and cel ...19695775904
sialidase-like enzymes produced by group a, b, c, g, and l streptococci and by streptococcus sanguis.a group of enzymes were prepared from the culture fluids of streptococci belonging to groups a, b, c, g, and l, and from a strain of streptococcus sanguis. these streptococcal enzymes (designated st-sialidases) released a substance shown to belong to the sialic acid group from the specific substrate bsm-st, a sialomucoid prepared from bovine submaxillary gland. they were inactive on n-acetylneuramin lactose prepared from bovine colustrum and on a sialomucoid prepared from bovine submaxillary muc ...19695776530
the effect of carbohydrate restriction on the presence of streptococcus mutans, streptococcus sanguis and iodophilic polysaccharide-producing bacteria in human dental plaque. 19705267941
adherence as a determinant of the presence of streptococcus salivarius and streptococcus sanguis on the human tooth surface. 19705275860
establishment of streptococcus sanguis in the mouths of infants. 19705280120
growth of streptococcus mutans and streptococcus sanguis in mixed culture. 19715284308
dextransucrase from streptococcus sanguis further characterization. 19715279546
ability of veillonella and neisseria species to attach to oral surfaces and their proportions present indigenously.the present study describes the distribution of veillonella and neisseria species in the human oral cavity and indicates that their ability to attach to oral surfaces correlates with their proportions found in various sites of the mouth. the mean percentages of veillonella and neisseria of the total flora cultivable on anaerobic blood-agar plates was found to be: plaque, 0.75 and <0.13, respectively; lip, 0.38 and <0.05; cheek, 0.66 and <0.14; tongue dorsum, 9.4 and <0.12; saliva, 5.0 and <0.9. ...19715154885
proportions of streptococcus sanguis, an organism associated with subacute bacterial endocarditis, in human feces and dental plaque.the relative absence of streptococcus sanguis in human feces, in contrast to its high concentration on teeth, supports the concept of the mouth as the likely bacterial source in cases of subacute bacterial endocarditis involving this organism.19715154899
saliva-induced aggregation of oral streptococci.cells of several species of oral microorganisms have been shown, in earlier studies, to be aggregated by saliva. in the present study some of the basic properties of the aggregation system are examined. the observation is made that the saliva-induced aggregates of streptococcus sanguis and s. mitis can be dissociated to stable particles which consist of about 100 cells and have a median diameter of about 4.5 mum. it is proposed that these are subunits, or core aggregates, of the large primary ag ...19724629653
[occurrence of neuraminidase and acylneuraminate pyruvate-lyase in streptococcus sanguis]. 19724146470
the occurrence of streptococcus mutans and streptococcus sanguis in the blood of endocarditis patients. 19724502189
establishment of defined microbial ecosystems in germ-free rats. i. the effect of the interactions of streptococcus mutans or streptococcus sanguis with veillonella alcalescens on plaque formation and caries activity. 19724504260
nutritional requirements of streptococcus sanguis. 19724506985
[interaction of soluble extracellular polysaccharides and concanavalin a produced by streptococcus mutans and streptococcus sanguis]. 19724513399
ecology of human oral lactobacilli.lactobacilli were found in saliva, on teeth, and on the dorsum of the tongue, the vestibular mucosa, and the hard palate in humans. their proportions in saliva, expressed as percentage of the anaerobically cultivable flora, were 10- to 100-fold higher than those on the tooth surface, but were comparable to those on the epithelial surfaces. the adherence of lactobacillus casei and l. fermenti to oral surfaces was compared with that of streptomycin-labeled streptococcus sanguis and s. salivarius b ...19724637297
properties of a streptococcus sanguis (group h) bacteriocin and its separation from the competence factor of transformation.sth(1), a streptocin elaborated by group h streptococcus strain challis, is lethal for group h streptococcus strain wicky and is produced maximally during the exponential growth phase of liquid medium cultures. crude streptocin preparations are resistant to oxidation and display a biphasic ph stability (stability being maximal at ph 5.0 and 10.0). survivor studies indicate that streptocin-mediated killing is a "one-hit" phenomenon and proceeds rapidly. the streptocin has been purified 50-fold wi ...19734725617
production of bactericidal concentrations of hydrogen peroxide by streptococcus sanguis. 19734515970
invertase activity in streptococcus mutans and streptococcus sanguis. 19734516065
recovery of streptococcus mutans and streptococcus sanguis from a dental explorer after clinical examination of single human teeth. 19734516070
regulation of dextransucrase formation by streptococcus sanguis. 19734518082
the effect of topical acidulated fluoride on percentage of streptococcus mutans and streptococcus sanguis in interproximal plaque samples. 19734518084
the localization and retention of 32p and 45ca within surface deposits of streptococcus sanguis and the influence of such deposits on the release of these isotopes from enamel. 19734518166
the effect of inoculating strains of streptococcus mutans and streptococcus sanguis upon caries incidence and bacterial content of plaque in rats. 19734518742
morphological changes in streptococcus sanguis associated with growth in the presence of oxygen. 19734518763
serology of streptococcus sanguis: localization of antigens with unlabeled antisera.a new technique for the electron microscope localization of bacterial surface antigens, using unlabeled antisera, has been developed. this technique, called the "immuno-coating reaction" was applied to serological studies of streptococcus sanguis, using antisera which had been shown to be specific for this species by gel diffusion analysis. the results of the immuno-coating reaction confirmed the specificity of the antisera for s. sanguis. in addition, the data indicate that some of the antigens ...19734199721
inhibition of enzymes by human salivary immunoglobulin a.human whole saliva inhibited bacterial neuraminidases and the inhibition was found to reside in the salivary iga fraction. further, salivary immunoglobulin (ig)a inhibited various bacterial enzymes and toxins: neuraminidases from streptococcus mitis, streptococcus sanguis, and clostridium perfringens, hyaluronidase and chondroitin sulfatase from oral bacteria, diphtheria toxin, and streptolysin o. the inhibitory activity of salivary iga did not correlate with that of serum on the basis of minimu ...19734354148
antigens of streptococcus sanguis.an antigenic analysis of the alpha-hemolytic streptococci isolated from dental plaque was performed by use of antisera against a strain of streptococcus sanguis (m-5) which was isolated from dental plaque. immunoelectrophoretic and ouchterlony tests of rantz and randall extracts of 45 strains gave positive reactions with the m-5 antisera. these strains represented 60% of the strains tested. the number of antigens which could be identified in these extracts varied from one to five and were design ...19734633291
induction of unbalanced growth and death of streptococcus sanguis by oxygen.streptococcus sanguis has normal streptococcal morphology when grown in a nitrogen atmosphere but undergoes gross morphological alterations when grown in the presence of oxygen (o cells). studies were made of the relationship between the development of pleomorphism and the loss of viability which accompanied it in o cultures of s. sanguis. the development of pleomorphism appears to involve two events. the first step, or triggering event, occurs in log-phase cultures in the presence of oxygen. th ...19734583207
efficiency of various growth media in recovering oral bacterial flora from human dental plaque.mm10 sucrose blood agar (mm10 sb agar), n(2)c agar, schaedler agar (sh agar), and mitis salivarius agar (ms agar) were tested for their ability to recover human dental plaque flora by a continuous anaerobic procedure and by a conventional anaerobic method. mm10 sb agar yielded higher recovery of bacteria from plaque samples as determined by the enumeration of colony-forming units (cfu). the cfu on n(2)c agar, sh agar, and ms agar were lower than mm10 sb agar when the continuous anaerobic procedu ...19734584588
identification of streptococcus sanguis by defined immunofluorescence. 19744601008
inhibition of transformation by sodium metaperiodate in streptococcus sanguis (wicky): demonstration that deoxyribonucleic acid binding sites contain amino acids.addition of sodium metaperiodate to competent streptococcus sanguis (wicky) cells at 0 or 37 c revealed that the deoxyribonucleic acid binding sites consist of amino acids.19744364331
turnover of phosphatidylglycerol in streptococcus sanguis. 19744370528
calcification of selected strains of streptococcus mutans and streptococcus sanguis.nine strains of cariogenic streptococcus mutans and two strains of streptococcus sanguis were tested for their ability to form hydroxyapatite. the cells were examined by x-ray diffraction and electron microscopy for apatite crystals after growth in a synthetic calcification medium. each of the test isolates, except for one strain of s. sanguis, produced intracellular mineral. two strains of s. mutans formed both intra- and extracellular crystals. there was no apparent relationship between calcif ...19744371107
differential susceptibility of human iga immunoglobulins to streptococcal iga protease.iga protease, a proteolytic enzyme found in human saliva and colonic fluid, hydrolyzes human serum iga immunoglobulins to yield fab(alpha) and fc(alpha) fragments. the enzyme is produced by organisms in the normal human microflora and can be purified from culture filtrates of the common human oral organism streptococcus sanguis (american type culture collection no. 10556). iga protease is inactive against all other protein substrates examined including the other classes of human immunoglobulins. ...19744436434
lipoteichoic acids from streptococcus sanguis.two lipoteichoic acids, membrane (mlta) and wall (wlta), have been purified from streptococcus sanguis by sepharose and ecteola-cellulose column chromatographies and concanavalin a-conjugated sepharose affinity column chromatography. the teichoic acids were homogenous as judged by disc gel electrophoresis, column chromatography, and double diffusion tests. both mlta and wlta consisted of glycerol, phosphate, glucose, and fatty acids in the ratios of 0.95:1:0.71:0.046 and 0.99:1:0.79:0.023, respe ...19744208135
glycerolphosphate-containing cell wall polysaccharides from streptococcus sanguis.six glycerolphosphate-containing tetraheteroglycans, a, b-1, b-2, b-3, b-4, and b-5, have been purified from the formamide extracts of streptococcus sanguis by alcohol and acetone precipitations, sephadex g-75, and diethylaminoethyl-cellulose column chromatography. the polysaccharides were judged as at least 95% pure by analytical disc gel electrophoresis and immune double diffusion against rabbit antiserum. they were shown to be cell wall polysaccharides, since they formed a single band of iden ...19744218231
a charged component in purified polysaccharide preparations from streptococcus mutans and streptococcus sanguis. 19744528382
cell-bound glucosyltransferase activity of streptococcus sanguis strain 804. 19744531859
the incidence and significance of streptococcus sanguis, streptococcus mutans and streptococcus salivarlius in root canal cultures from human teeth. 19744532212
carbon dioxide control of lag period and growth of streptococcus sanguis.a carbon dioxide requirement for growth of streptococcus sanguis was readily demonstrated in a fermentor where the gas atmosphere could be controlled. growth at a maximum rate occurred immediately in response to the appropriate co(2) concentration; growth stopped when co(2) was deleted. washed inocula consisting of exponentially growing cells required a minimum of 2.4% co(2), postexponential phase cells needed 1.2 to 1.8% co(2) immediately and 2.4% co(2) shortly thereafter, whereas stationary ph ...19744811542
neuraminidase activity in streptococcus sanguis and in the viridans group, and occurrence of acylneuraminate lyase in viridans organisms isolated from patients with septicemia.the enzyme neuraminidase (ec 3.2.1.18) was found to be strongly active in different types of streptococcus sanguis and s. viridans, and, in addition, the occurrence of the enzyme acylneuraminate pyruvate lyase (ec 4.1.3.3) was described in s. viridans. the enzyme-active bacteria strains were isolated from blood cultures of patients with septicemia. whereas s. sanguis lost its strong neuraminidase activity after some weeks, s. viridans retained its enzyme activity for a long time in culture. immu ...19744816461
alteration of macromolecular synthesis and membrane permeability by a streptococcus sanguis bacteriocin. 19744822122
inhibition of the development of competence in streptococcus sanguis (wicky) by reagents that interact with sulfhydryl groups: discernment of the competence process.reagents that interact with sulfhydryl groups are shown to inhibit competence factor (cf)-induced competence development in streptococcus sanguis (wicky) strain we4 (wicky 4 ery(r)). inhibition is correlated with specific inhibition of either the function or biosynthesis of three competent cell-related proteins and is reversed by either 2-mercaptoethanol or dithiothreitol. mercuric chloride (5 mum) or n-ethylmaleimide (nem; 50 mum) inhibited (i) the function but not the biosynthesis or activatio ...19744829923
glycogen accumulation by pleomorphic cells of streptococcus sanguis. 19744830758
isolation of an enzyme from streptococcus sanguis which specifically cleaves iga. 19744832315
in vitro attachment of streptococci to the tooth surface.the ability of streptococcus strains to adhere to the tooth surface in vitro was investigated. polished enamel slabs, with and without acquired pellicles, were incubated with buffer suspensions of oral streptococci, and attached bacteria were counted under a microscope using incident light. low numbers of bacteria adhered to uncoated enamel; the presence of an acquired pellicle significantly enhanced the attachment of all strains tested. the adherence of streptococcus sanguis was significantly g ...19744856824
relation of macromolecular synthesis in streptococci to efficiency of transformation by markers of homospecific and heterospecific origin.in previous studies with streptococcus sanguis and s. pneumoniae as recipients and donors of transforming deoxyribonucleic acid (dna), it was found that heating recipients just prior to exposure to dna caused an increase in the number of transformants induced by heterospecific dna relative to that induced by homospecific dna. in the present studies, s. sanguis recipients were found to recover from this effect of heat (48 c, 15 min) when incubated at 37 c before exposure to dna. inhibitors of nuc ...19744130687
mutanolysin, bacteriolytic agent for cariogenic streptococci: partial purification and properties.mutanolysin partially purified from the culture filtrate of streptomyces globisporus 1829 consists of two main lytic enzymes with an isoelectric point near ph 8.5 and 10, respectively, and proteolytic enzyme is associated with the latter lytic enzyme. mutanolysin exhibited maximal lytic activity at 60 c in the ph range 6.5 to 7.0 and was stable at 50 c in the acid range. n-bromosuccinimide caused complete inhibition of lytic activity at 1 mm, whereas calcium and magnesium ions at the same concen ...197415828186
isolation and purification of flavobacterium alpha-1,3-glucanase-hydrolyzing, insoluble, sticky glucan of streptococcus mutans.studies were made on the physical and chemical properties of polysaccharides synthesized by cell-free extracts of streptococcus mutans, streptococcus sanguis, and streptococcus sp. and their susceptibilities to dextranases. among the polysaccharides examined, insoluble glucans were rather resistant to available dextranase preparations, and the insoluble, sticky glucan produced by s. mutans omz 176, which could be important in formation of dental plaques, was the most resistant. by enrichment cul ...1975370
genetic hybridization at the unlinked thy and str loci of streptococcus.the sanguis and pneumoniae species of streptococcus were used as recipients in transformations from str+ to str-r and from thy- to thy+. the str-r mutations in the two species had been previously shown to be allelic. homology of the thy- mutations in the two species was demonstrated in the similar phenotypic properties they conferred (death in the absence of thymidine, lack of thymidylate synthetase). the str and thy loci are unlinked in each species.--- when the two species are transformed by b ...19751325
effect of actinomyces viscosus on the establishment and symbiosis of streptococcus mutans and streptococcus sanguis in spf rats on different sucrose diets. 1975123171
effect of polymyxin b on liposomal membranes derived from escherichia coli lipids.the specificity of the action of polymyxin b was studied using liposomes as a model membrane system. liposomes prepared from total lipids of gram-negative bacteria escherichia coli, a mixture of purified e. coli phosphatidylethanolamine and cardiolipin and a mixture of phosphatidylethanolamine and phosphatidylglycerol, were extemely sensitive to polymyxin while those prepared from lipids of gram-positive bacteria streptococcus sanguis, lipids of sheep erythrocyte membranes, mixtures of egg lecit ...1975163098
the role of transamination in ammonium assimilation in streptococcus sanguis. 1975238495
soluble macromolecular complexes involving bacterial teichoic acids.cell wall and membrane teichoic acids from several bacteria formed soluble complexes with polysaccharides and bovine plasma in alkyl alcohol solutions. polysaccharides which contain different monomeric units and anomeric configurations complexed with the teichoic acids, suggesting that the interaction is relatively nonspecific. teichoic acids complexed glycogen or bovine plasma albumin in 50 to 97% ethanol solutions. the macromolecular association between teichoic acids and polysaccharides or pr ...1975240807
glucose-6-phosphate-dependent pyruvate kinase in streptococcus mutans.pyruvate kinase of streptococcus mutans jc 2 had an absolute and specific requirement for glucose-6-phosphate. inorganic phosphate was a strong inhibitor. the enzyme required k+ or nh4+ and mg2+ or mn2+. s. mutans fil and e 49, streptococcus bovis atcc 9809, and streptococcus salivarius atcc 13419 had also glucose-6-phosphate-dependent pyruvate kinases, whereas streptococcus sanguis nctc 10904 had an enzyme activated by fructose-1,6-diphosphate.1975240810
studies on the bacterial components which bind streptococcus sanguis and streptococcus mutans to hydroxyapatite. 1975241311
recovery and clearance rates of oral microorganisms following experimental bacteraemias in dogs. 1975242303
structural characteristics of native and enzymically formed dextran of s. sanguis atcc 10558.the structure of the native dextran produced by streptococcus sanguis atcc 10558 was analyzed by g.l.c.-m.s. of the methylated alditol acetates derived from the polymer. the results indicate that the polymer contains d-glucosyl residues substituted at c-6 or c-3, or both, as well as unsubstituted d-glucosyl residues. these data aially purified dextransucrase on sucrose. the proportion of d-glucosyl residues substituted at c-3 is diminished in this case. it is concluded that several enzymes are i ...19751139563
a method for gentle lysis of streptococcus sanguis and streptococcus mutans. 19751147995
chemotherapy of experimental streptococcal endocarditis. iv. further observations on prophylaxis.the ability of antibiotics to prevent streptococcus sanguis endocarditis was tested in rabbits. only vancomycin or a combination of penicillin g plus streptomycin always prevented infection when administered as a single dose. a loading dose of 30 mg/kg of phenoxymethyl penicillin (penicillin v) followed by additional 7.5 mg/kg doses for 48 h proved to be the only successful prophylactic program that could be given orally to man. cefazolin alone or with streptomycin in multiple doses was also an ...19751150874
immunoelectron microscopic identification and localization of streptococcus sanguis with peroxidase-labeled antibody: localization of streptococcus sanguis in intact dental plaque.streptococcus sanguis has been localized ultrastructurally within intact dental plaque by means of an indirect technique which utilizes horseradish peroxidase-labeled antibody. the technique allows for complete diffusion of the reagents to all portions of the plaque specimens. control procedures can be carried out on serial sections of plaque with a bacterial composition similar to that of the experimental specimen. the 30-mum-thick sections can be examined in the light microscope to localize ar ...19751167846
inhibition of streptococcal attachment to receptors on human buccal epithelial cells by antigenically similar salivary glycoproteins.preparations of salivary glycoproteins inhibited the attachment of certain indigenous oral streptococci to human buccal epithelial cells and fostered the desorption of previously attached bacteria. the adherence-inhibiting and desorptive activities of the glycoproteins correlated with their ability to aggregate these organisms. pretreatment of glycoprotein preparations with certain blood group antisera impaired their adherence-inhibiting effect, suggesting that components with blood group substa ...19751168169
evaluation of human oral organisms and pathogenic streptococcus for production of iga protease.iga protease is a proteolytic enzyme found in whole human saliva and in dental plaque that cleaves both secretory and myeloma iga of human origin to yield intact fabalpha and fcalpha fragments. to determine which bacteria are capable of producing this enzyme, we have examined a variety of strains normally found in the human oral cavity and a number of streptococci of known lancefield group serotype. streptococci of groups a, b, c, d, f, g, h, m, and n, streptococcus mutans, streptococcus sanguis ...19751168676
twitching motility and possession of polar fimbriae in spreading streptococcus sanguis isolates from the human throat.a collection of 19 strains of alpha haemolytic streptococci, isolated from throat swabs and characterized by production of spreading zones around colonies on blood agar, was found to constitute a very homogeneous group with morphological, physiological and biochemical characters corresponding to those of streptococci of ser-group h, or streptococcus sanguis, and they all appeared to possess the group h antigen. they all had a common agglutinogen and, in addition, heterogeneous agglutinogens. the ...19751171576
dna base sequence homologies among strains of streptococcus sanguis.dna was isolated from 19 strains and substrains of streptococcus sanguis and analysed for guanine plus cytosine (gc) contents and base sequence homologies. three groups could be discerned: group 1 strains had 40-8 to 42-8 mol % gc; group 2, 42-7 to 44-0 mol % gc; group 3, 43-8 to 46-4 mol % gc. dna homologies between groups 1 and 3 were 40 to 60% at 67 degrees c and 40% at 72 degrees c. the homologies of group 2 towards groups 1 and 3 were much lower. strains in groups 1 and 3 hydrolysed arginin ...19751202161
a transforming marker that produces merodiploids with high efficiency and stable transformants with low efficiency in streptococcus.a mutation (ery-r8) conferring a high level of resistance to erythromycin in the challis strain of streptoccus sanguis can be transferred to wild-type erythromycin-sensitive recipients via single molecules of donor dna. the transformants thus produced are of two types: (1) cells slightly more resistant to erythromycin than wild-type and capable of segregating (at a frequency of 2 x 10(-4)/bacterium/generation) either wild-type or highly-resistant cells like the original donor type; (2) cells phe ...19751232022
amino acid requirements and proteolytic activity of streptococcus sanguis.the growth response of streptococcus sanguis groups 1:a and 1:b in a complete chemically defined medium was not influenced by the oxygen concentration of the growth atmosphere. all of the cultures required cysteine and arginine; tyrosine and branched-chain amino acids were frequently required. proteolysis of casein, mucin, and the anionic proteins of germfree rat saliva by s. sanguis was demonstrated. hydrolytic activity toward casein was found in the soluble contents of the cells and in the cel ...19751237268
the reaction of salivary substances with bacteria.reactions between selected strains of oral streptococci and saliva were studied spectrophotometrically. the curves of absorbance (700 nm) versus those of time were sigmoidal for aggregating saliva-bacteria mixtures. whtn the logarithm of the ratio of the change in absorbance to the remaining absorbance was plotted against the reaction time, straight lines were obtained. at a fixed concentration of bacteria, the slope of these lines for a series of dilutions of a selected saliva sample was a para ...1975817003
preparation of protoplasts of group h streptococci (streptococcus sanguis).stable protoplasts of several strains of group h streptococci (streptococcus sanguis) can be prepared by use of group c streptococcal phage-associated lysin in the presence of 30% raffinose. sucrose cannot be substituted for raffinose. protoplast formation did not require the addition of mg2+; however, this cation enhanced their stability. some other strains, also presumptive group h streptococci, were not sensitive to phage-associated lysin.1975803288
characterization of an antibody directed against a surface component of normal and pleomorphic cells of streptococcus sanguis.whole cells of streptococcus sanguis were utilized as an immunoadsorbent to purify large quantities of an antibody (s1) directed against a cell surface component. the s-1 antibody was isolated from antisera to normal (n) and pleomorphic (o) cells by a similar adsorption-elution procedure. the s-1 antibody isolated from antisera to n cells reacted in gel diffusion in identify with the s-1 antibody to o cells, indicating that the antigen which binds s-1 antibody (ag-1) may not be radically altered ...1975809362
streptococcus salivarius, streptococcus sanguis and streptococcus "mitis" as indicators of contamination of root canal cultures.small samples were taken from the first drop of saliva entering an operation field for root canal treatment isolated by rubber dam, which was intentionally leaky. the samples were primarly cultured in tas- and blh-medium and on ms-agar aerobically. the culture media were inoculated directly and via transport medium (vmg iii). the cultures were analysed for strep. salivarius, strep. sanguis and/or strep. "mitis" after subculture on ms-agar as soon as visible growth had appeared in the broth media ...19751057108
variations in microbial and biochemical components of four-day plaque during a four-week controlled diet period.variation in microbial and biochemical components of human four-day plaque was studied in seven subjects who were maintained on a high-sucrose diet during a four-week period. significant changes in populations of lactobacilli dextranase-producing organisms, streptococcus mutans, and s sanguis occurred during this period.19751057555
investigation of possible solvents for extracellular polysaccharides from streptococcus mutans and streptococcus sanguis.the solubilities of extracellular polysaccharide fractions produced by 14 oral streptococcal strains were compared in water, aqueous lithium and guanidine salt solutions, dimethyl sulfoxide (dmso), and a 9:1 dmso-water mixture. the best results for solubilizing the fractions were obtained with the dmso-water mixture procedure, which used the water and dmso in sequence. by this method all fo the fractions were solubilized.19751057570
effects of dietary sucrose levels on the quantity and microbial composition of human dental plaque.the effects of dietary sucrose levels on the microbial composition of 4- and 12-day plaque were investigated in eight dental students. sucrose-rich diets had no demonstrable effects on total plaque accumulation, whereas total viable microbial density, streptococcus mutans, and the lactobacilli populations increased. the s sanguis population was unaffected. however, in individual subjects, an inverse relationship between the s sanguis and s mutans population was observed.19751057572
[extracellular polysaccharides and caries activity in a gnotobiotic system for lysogenic and cured streptococci]. 19751058183
relationship between volume and streptococcal population in dental plaque. 19751058868
the effects of sugar. 19751065037
effect of topical acidulated phosphate fluoride on percentage of streptococcus mutans and streptococcus sanguis in plaque. ii. pooled occulusal and pooled approximal samples. 19751090368
immunoelectron microscopic identification and localization of streptococcus sanguis with peroxidase-labeled antibody: localization of surface antigens in pure cultures.an indirect method of localizing antigens with horseradish peroxidase-labeled antibody was used to identify and localize surface antigens of streptococcus sanguis at the ultrastructural level. an electron dense layer surrounding the cell wall could be distinguished without any additional electron microscope staining. this labeled layer represents an immune complex consisting of bacterial surface antigens, specific rabbit antisera, and peroxidase-labeled goat anti-rabbit globulins. although with ...19751090524
growth of several cariogenic strains of oral streptococci in a chemically defined medium.a chemically defined medium in which streptococcus mutans strains aht, bht, gs-5, jc-2, ingbritt, at6t, at9t, 6715, and omz-176 and streptococcus salivarius strain hht grew rapidly to high turbidities was formulated. maximal turbidities of each strain were observed after 8 to 12 h of aerobic growth. the subsequent transfer of exponentially growing cells into fresh medium resulted in growth at the same rate without lag. growth of these strains occurred with rates at least one-half of those observ ...19751091546
amino acid requirements of streptococcus mutans and other oral streptococci.the amino acid requirements of streptococcus mutans strains aht, omz-61, fa-1, bht, gs-5, jc-2, ingbritt, at6t, omz-176, 6715, streptococcus salivarius hht, streptococcus sanguis omz-9, and strain 72x46 were determined in a chemically defined medium. when grown anaerobically in the presence of sodium carbonate (or bicarbonate for a few strains), few amino acids were required. all strains tested required cystine (or cystine) as a nutrient. three strains (s. mutans omz-176, fa-1, and bht) required ...19751091547
chemical composition of the washed cells of streptococcus sanguis (804) and streptococcus mutans (b-14).washed cells of streptococcus saguis (804) and streptococcus mutans (b14) were analyzed for water, ash, protein, carbohydrate, lipid, and nucleic acid content. lipids were analyzed by gas chromatography. ash was subjected to elemental analysis. these data were compared with the published values for the composition of dental plaque.19751095621
[nasopharyngeal microorganisms--antagonists of meningococci].a study was made of the species composition of symbiotic microbial flora of the nasopharynx in healthy persons and meningococcus carriers, inhibiting in vitro the growth of the test strains of miningococci. saprophytic streptococci and particularly str. salivarius served as the principal and the most active antagonists of meningococci in both groups of the persons examined. comparison of the inhibitory activity of antagonists in the groups examined revealed no significant differences. however, t ...1975816119
immunologic basis for vaccination against dental caries in rhesus monkeys.the effects of immunization with streptococcus mutans on the development of caries and the immune responses were investigated in 37 young rhesus monkeys (macaca mulatta) during a period of up to 33 months. the monkeys were supplied a human type of carbohydrate-rich diet that contained about 15% sucrose. the monkeys were separated into seven groups, and the effects of two whole cell vaccines and an extracellular culture extract of s mutans in freund's incomplete adjuvant were compared with a vacc ...1976816834
strain typing of oral streptococci by the use of bacterial antagonism.strain-typing methods utilizing microbial antagonism have been developed for s sanguis and for s mutans. the inhibition observed resembled that of bacteriocins. inhibition within the species s sanguis was obtained with group d streptococci and facultative members of the genus bacillus. thirty-three inhibitors were paired (not in all possible combinations) with 37 isolates of s sanguis. depending on the inhibitor, growth of between 15 and 85% of the sensitive indicators was halted. three of the i ...19761107377
in vitro attachment of streptococcus sanguis to dental crown and bridge cements.the ability of a common dental plaque bacterium, streptococcus sanguis, to adhere to dental crown and bridge cements in vitro was investigated. cylindrical blocks of five different commercial brands of cement, with and without acquired pellicles, were indubated with buffer suspensions of s. sanguis for 1 h. attached bacteria were counted under the microscope. s. sanguis had particularly high affinity for uncoated resin cement. in contrast, the carboxylate cement tested was a poor substrate for t ...19761066446
[production of dental plaque in vitro in extracts of various food products]. 19761067165
fluoride accumulation by a strain of human oral streptococcus sanguis. 19761067796
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