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characterization and identification of coagulase-negative, heat-stable deoxyribonuclease-positive staphylococci.various characteristics of 13 coagulase-negative, weakly heat-stable deoxyribonuclease-positive staphylococci from human, veterinary and food sources were determined in an effort to identify them. nine of the isolates were identified as coagulase-negative staphylococcus aureus (2), staphylococcus xylosus (2), staphylococcus simulans (3), staphylococcus capitis (1) and staphylococcus sciuri subsp. lentus (1); the other four isolates, from food and veterinary sources, could not be identified as cu ...1978641525
modified oxidase and benzidine tests for separation of staphylococci from micrococci.two simple and rapid methods for the separation of staphylococci from micrococci are described. they are based on modified oxidase and benzidine tests. micrococci and staphylococcus sciuri yield a blue color with a 6% solution of tetramethylphenylenediamine in dimethyl sulfoxide, whereas all of the other staphylococci exhibit no coloration. best's results were obtained with overnight cultures on blood agar. the presence of c-type cytochromes i micrococci and s. sciuri could be detected with benz ...19817019234
plasmid composition of staphylococcus species.a total of 342 staphylococci representing 13 different recognized species were screened for plasmid composition using agarose gel electrophoresis techniques. plasmids of molecular weight (mw) 30 x 10(6) or larger in size were uncommon in staphylococci. moderately large plasmids of mw 15 x 10(6) to 29 x 10(6) were not or only occasionally found in the species staphylococcus sciuri, s. intermedius, s. hyicus, or s. simulans, but were common in s. aureus (55%) and members of the s. epidermidis (79% ...19817237278
biotypes of staphylococcus epidermidis and micrococcus organisms, isolated from intramammary infections, reclassified into species of the genus staphylococcus (epidermidis, hyicus, xylosus, and sciuri).in a previous report (5), strains of staphylococcus epidermidis and micrococcus isolated from bovine intramammary infections were classified by the baird-parker (b-p) system and by serologic typing of the proteolytic enzymes. since then, newer methods for distinguishing the genera staphylococcus and micrococcus and for defining new species have been reported. by utilizing these methods, the organisms from the original study were reclassified as follows: s. epidermidis b-p subgroup ii, proteinase ...19836340953
elevation of staphylococcus sciuri subsp. lentus (kloos et al.) to species status: staphylococcus lentus (kloos et al.) comb. nov.deoxyribonucleic acid (dna)-dna hybridization and physiological studies on 15 strains of s. sciuri subsp. lentus and 4 strains of s. sciuri subsp. sciuri demonstrated that species rank should be conferred on these subspecies. strain atcc 29070, the type strain of s. sciuri subsp. lentus, is proposed as type strain of s. lentus. an emended description of this strain is given.198323194736
staphylococcus sciuri subsp lentus associated with goat mastitis.an organism recovered from mastitic milks of goats was identified as staphylococcus sciuri subsp lentus. isolation of this organism in pure cultures from individual half-udder samples of 5 goats of 18 affected, confirms that it possesses pathogenic potential.19846497107
characteristics of some unclassifiable strains of staphylococci isolated from goats and sheep.fourteen strains of catalase-positive, gram-positive and coagulase-negative cocci that were sensitive to 20 micrograms/ml of furazolidone were isolated from goats and sheep. two of the strains had glycerol with glucose teichoic acids whilst another possessed glycerol, glucose, glucosamine and acetylglucosamine teichoic acids. six strains had peptidoglycan type l-lys-ala-gly4 peculiar to staphylococcus sciuri and staph. lentus but other phenotypic characters were different from those of staph. sc ...19854055538
comparative characteristics of staphylococcus sciuri, staphylococcus lentus and staphylococcus gallinarum isolated from healthy and sick hosts.of 136 strains of coagulase-negative staphylococci isolated from healthy and sick human beings, goats, sheep, antelope and other animals, 88 (64.7%) were staphylococcus sciuri and 35 (25.7%) were s. lentus and the remainder staphylococcus gallinarum. the strains of s. sciuri were isolated from humans with boils and wounds, goats with pestes des petits ruminants (ppr) and dogs with nasal discharge. one isolate of s. gallinarum came from a fowl with chronic respiratory disease and 11 others were i ...19863705446
bacteriology of the oral cavity of balb/c mice.to be used as a model in dental research, an animal must fulfil experimental needs and information on the composition and variation of its oral flora must be available. only limited data are available on the indigenous oral bacterial flora of balb/c mice. in this work, a total of 671 isolates from different sites (saliva, tongue, teeth, and mucosa) of the oral cavity of balb/c mice were identified. only 18 different species were isolated, which indicates the relative simplicity of the flora. the ...19863768807
transport, nutritional and metabolic studies of taurine in staphylococci.a specific, na+-dependent, energy-requiring transport system for taurine has been reported recently in the staphylococcus aureus m strain. taurine was taken up vigorously by all s. aureus strains tested. the system was na+-dependent, and na+ decreased the km but had no effect on the vmax of the transport system. among coagulase-negative staphylococci, the staphylococcus epidermidis group (a taxonomically related group of species associated with humans or other primates) and the free-living, wide ...19873655734
can the association of athymic mice (han:nmri-nu) with staphylococcus sciuri prevent infection with staphylococcus aureus? experiences from a field study.an attempt was made to prevent the introduction of staphylococcus aureus into a newly established colony of han:nmri-nu mice by means of preassociation with the rodent-specific staphylococcus sciuri. despite the successful colonization of the mice with s. sciuri the establishment of s. aureus into the colony was not impeded, and abscesses were observed with an increasing frequency until the end of the study. random samples revealed the phage pattern 3a/3c/55/71 or very similar ones. a caretaker ...19892523626
[changes of bacterial flora on inbred mouse strains using experimental dental caries model].in the experimental dental caries model using inbred mouse strains, the bacterial flora of lower molars were investigated during the process of caries induction to examine the differences of bacterial flora between the caries susceptive mouse strains (balb/ca, c57bl/6n) and the caries resistant mouse strain (c3h/hen), as well as the relation between the bacterial flora and streptococcus faecalis that seemed to be the pathogen of caries on this caries model. the total cultivable bacterial number ...19892626795
detection of a novel chloramphenicol resistance plasmid from "equine" staphylococcus sciuri.a small chloramphenicol resistance (cm) plasmid of 4.65 kb could be detected in an "equine" staphylococcus sciuri-culture. this plasmid, designated as psc3, was identified by interspecific protoplast transformation. on the basis of restriction endonuclease analyses a detailed restriction map of psc3 could be constructed. this allowed structural comparisons of psc3 with cm-plasmids of other staphylococcal species from infections of humans and animals and identification of psc3 as a member of the ...19902267890
a chloramphenicol-streptomycin-resistance plasmid from a clinical strain of staphylococcus sciuri and its structural relationships to other staphylococcal resistance plasmids.a 5.1-kb plasmid, designated pscs12, isolated from a naturally occurring staphylococcus sciuri conferred resistance to chloramphenicol (cmr) and streptomycin (smr). restriction endonuclease analyses of pscs12 revealed partial structural homologies to the cmr-plasmids pc221 from s. aureus and pscs1 from s. intermedius, to the smr-plasmids psai-1 from s. hyicus and ps194 from s. aureus, as well as to the cmr/smr plasmid psk68 from s. aureus. southern-blot hybridization with specific cmr- and smr-g ...19911769526
identification of methicillin-resistant strains of staphylococci by polymerase chain reaction.a simple and reliable method using a polymerase chain reaction (pcr) was devised to identify methicillin-resistant staphylococci. by using lysates of the strain to be tested as templates and 22-mer oligonucleotides as primers, a 533-bp region of meca, the structural gene of a low-affinity penicillin-binding protein (pbp 2'), was amplified by pcr and detected by agarose gel electrophoresis. results obtained by this method were compared with those obtained by broth microdilution mic determination ...19911939577
a convenient method for differentiation of coagulase-negative staphylococci isolated from bovine mammary glands.the utility of trehalose-mannitol broth and arabinose-cellobiose broth for identification of staphylococcus epidermidis and novobiocin-resistant staphylococci was determined using 236 coagulase-negative staphylococci isolated from bovine mammary glands. none of the 49 s. epidermidis strains was positive in trehalose-mannitol broth; whereas, all strains of staphylococcus hyicus, staphylococcus chromogenes, staphylococcus haemolyticus, staphylococcus hominis, staphylococcus warneri, and staphyloco ...19912045549
staphylococcus muscae, a new species isolated from flies.a new coagulase-negative species of the genus staphylococcus, staphylococcus muscae, is described on the basis of the results of a study of four strains that were isolated from flies. 16s rrna sequences of the type strains of s. muscae, staphylococcus schleiferi, and staphylococcus sciuri were determined and used, together with the corresponding sequences of staphylococcus aureus and staphylococcus epidermidis, for a comparative analysis. the new species is characterized taxonomically; this spec ...19921371067
identification of the staphylococcus sciuri species group with ecori fragments containing rrna sequences and description of staphylococcus vitulus sp. nov.strains of a new species, staphylococcus vitulus, were isolated from food and a variety of mammals. this species was recognized on the basis of the results of an analysis of genomic ecori fragments containing portions of the rrna operons. the patterns of hybridized fragments obtained from strains belonging to the new taxon were sorted into a distinguishable cluster and were distinct from the staphylococcus lentus and staphylococcus sciuri patterns. the results of dna-dna hybridization reactions ...19947520736
structure and putative origin of a plasmid from staphylococcus hyicus that mediates chloramphenicol and streptomycin resistance.the structure and functional organization of staphylococcus hyicus plasmid pscgp3eb that mediates chloramphenicol and streptomycin resistance (cmrsmr) is described and compared with another cmrsmr plasmid, pscs12, from staphylococcus sciuri. both plasmids appeared to be formed by co-integrate formation between plasmids that very closely resemble the chloramphenicol resistance (cmr) plasmid pc221 and the streptomycin resistance (smr) plasmid ps194. in addition to the established recombination sit ...19947764811
characterization of the most frequently encountered staphylococcus sp. in cats.ninety three staphylococci isolated from clinical specimens from cats were characterized and identified. because the biochemical characteristics of staphylococcus felis were very similar to those of staphylococcus simulans, results were submitted to numerical analysis and dna homology. forty-two isolates (45%) were identified as s. felis, and 4 isolates (4%) as s. simulans. the other species identified, in order of their frequency were, 12 staphylococcus aureus (13%), 9 staphylococcus intermediu ...19948042273
antimicrobial susceptibility of ruminal coagulase-negative staphylococci.the species staphylococcus sciuri subsp. sciuri, staphylococcus sciuri subsp. lentus, staphylococcus saprophyticus and staphylococcus xylosus of novobiocin resistant coagulase-negative staphylococci (cns) isolated from rumen content or wall of lambs and calves were determined. all bacteria were tested for lactic acid production, urease, adherence activities and antimicrobial susceptibility or resistance. the majority of strains presented belonged to bacteria with low affinity for epithelial cell ...19948065269
isolation of methicillin-resistant coagulase-negative staphylococci from chickens.methicillin-resistant coagulase-negative staphylococci were isolated from the nares and skin of 1- to 8-week-old healthy chickens in three flocks from a farm. isolation of methicillin-resistant coagulase-negative staphylococci was positive for 72 (25.7%) of the 280 chickens tested, with the frequency varying from 2.2 to 100% according to flock. a total of 45 appropriate isolates were selected and subjected to identification. of the 45 methicillin-resistant coagulase-negative staphylococcal isola ...19968862560
ubiquitous presence of a meca homologue in natural isolates of staphylococcus sciuri.in an effort to explore the origin and/or reservoirs of the genetic determinant(s) of methicillin resistance in staphylococcus aureus, we examined over 200 strains representing 13 different species within the genus staphylococcus for the presence of the meca gene, using a dna probe internal to this gene prepared from a methicillin-resistant strain of s. aureus. occasional meca- positive isolates were detected among several staphylococcal species. on the other hand, each one of the 134 isolates o ...19969158808
tracking the evolutionary origin of the methicillin resistance gene: cloning and sequencing of a homologue of meca from a methicillin susceptible strain of staphylococcus sciuri.the gene meca, a central genetic determinant of methicillin resistance in staphylococcus aureus and coagulase-negative staphylococci of human origin, has an unknown extra species origin in these human pathogens. after screening isolates representing over 15 different species within the genus staphylococcus we could identify only one--s. sciuri--in which each of over 150 independent isolates showed positive hybridization with a meca-specific dna probe isolated from a methicillin-resistant strain ...19969158816
nitrate-reducing bacteria on rat tongues.nitrite-producing bacteria (npb) were isolated from tongues of laboratory rats. the most commonly found nitrite-producing organism was staphylococcus sciuri, followed by staphylococcus intermedius, pasteurella spp., and finally streptococcus spp. both morphometric quantification of bacteria on tongue sections and enumeration of culturable bacteria (cfu) showed an increase in the density of bacteria towards the posterior tongue. up to 65% of bacteria were located in the deep clefts on the posteri ...19979055411
unusual recovery of animal staphylococci from septic wounds of hospital patients in ile-ife, nigeria.two staphylococcus species--staphylococcus gallinarum and staphylococcus sciuri--previously known to be of animal origin have been isolated from infected wounds of hospital patients in ile-ife, nigeria. these organisms were non-typable at both rtd and 100 x rtd and did not produce beta-lactamase detectable by the normal methods. their actual involvement in the infection is not immediately clear, but their resistance to most common antibiotics, in addition to the production of dnase and phosphata ...19979081309
ribotype delineation and description of staphylococcus sciuri subspecies and their potential as reservoirs of methicillin resistance and staphylolytic enzyme genes.three subspecies of staphylococcus sciuri, s. sciuri subsp. sciuri kloos, schleifer, and smith 1976, 23al emend. kloos et al. 1997 [corrected], s. sciuri subsp. carnaticus subsp. nov., and s. sciuri subsp. rodentium subsp. nov., are described on the basis of their ribotype patterns, dna-dna liquid hybridization data, and phenotypic characteristics. normalized ribotyping subdivided the s. sciuri patterns into three blocks of patterns, each corresponding to a subspecies. each subspecies formed a s ...19979103615
purification of an extracellular proteinase from staphylococcus sciuri found on the surface of tilsit cheese. 199810909787
genetic organization of the meca region in methicillin-susceptible and methicillin-resistant strains of staphylococcus sciuri.a homolog of the staphylococcus aureus methicillin resistance gene meca was recently shown to be ubiquitous in independent isolates of the animal species staphylococcus sciuri. the meca gene homolog and regions flanking it were cloned and sequenced from four strains of s. sciuri: strain k1 (atcc 29062), a representative of s. sciuri subsp. sciuri; two strains (k3 and k8) representing s. sciuri subsp. rodentius; and strain k11, a representative of s. sciuri subsp. carnaticum. strains k1 and k11 w ...19989440511
delimiting the genus staphylococcus through description of macrococcus caseolyticus gen. nov., comb. nov. and macrococcus equipercicus sp. nov., and macrococcus bovicus sp. no. and macrococcus carouselicus sp. nov.four species of the newly proposed genus macrococcus, namely macrococcus caseolyticus gen. nov., comb. nov. (formerly staphylococcus caseolyticus schleifer, kilpper-bälz, fischer, faller and endl 1982, 19vp), macrococcus equipercicus sp. nov., macrococcus bovicus sp. nov. macrococcus carouselicus sp. nov., are described on the basis of a phylogenetic analysis comparing 16s rrna sequences, dna-dna liquid hybridization, dna base composition, normalized ribotype patterns, macrorestriction pattern a ...19989734040
endocarditis due to staphylococcus sciuri. 19989832275
penicillin-binding protein-mediated resistance in pneumococci and staphylococci.target alteration underlies resistance to beta-lactam antibiotics in both staphylococcus species and streptococcus pneumoniae. the penicillin-binding protein (pbp) targets in penicillin-resistant strains of s. pneumoniae are modified, low-binding-affinity versions of the native pbps. multiple pbp targets may be modified by transformation and homologous recombination with dna from pbp genes of viridans streptococci. the level of resistance is determined by how many and to what extent targets are ...199910081507
distribution of staphylococcus sciuri subspecies among human clinical specimens, and profile of antibiotic resistance.the distribution of three subspecies comprising staphylococcus sciuri was determined for a collection of 30 clinical isolates originating from morocco, the united kingdom, and france. the sources of these isolates were principally wounds, skin, and soft tissue infections. at the species level, the isolates were identified according to biochemical characteristics and at the subspecies level by the ribotyping technique. pcr analysis performed with the 16s-23s ribosomal dna intergenic spacer was le ...199910577486
dietary nitrate in man: friend or foe?based on the premise that dietary nitrate is detrimental to human health, increasingly stringent regulations are being instituted to lower nitrate levels in food and water. not only does this pose a financial challenge to water boards and a threat to vegetable production in northern europe, but also may be eliminating an important non-immune mechanism for host defence. until recently nitrate was perceived as a purely harmful dietary component which causes infantile methaemoglobinaemia, carcinoge ...199910615207
intestinal protozoa and associated bacteria in captive houbara bustards (chlamydotis undulata) in the united arab emirates.a protozoal survey was carried out in 114 captive houbara bustards (chlamydotis undulata) in the united arab emirates. trichomonas gallinarum, chilomastix gallinarum, giardia spp. and lophomonas spp. were detected from faecal samples of 49 (43%) captive houbara bustards. culture and identification studies were carried out to assess associated bacteria. the bacteria isolated from birds negative to protozoa included enterococcus spp., aerococcus spp., micrococci spp., proteus spp., enterobacter sp ...199916147554
molecular characterization of staphylococcus sciuri strains isolated from humans.we previously characterized over 100 staphylococcus sciuri isolates, mainly of animal origin, and found that they all carried a genetic element (s. sciuri meca) closely related to the meca gene of methicillin-resistant staphylococcus aureus (mrsa) strains. we also found a few isolates that carried a second copy of the gene, identical to mrsa meca. in this work, we analyzed a collection of 28 s. sciuri strains isolated from both healthy and hospitalized individuals. this was a relatively heteroge ...200010699009
use of an enzyme-linked lectinsorbent assay to monitor the shift in polysaccharide composition in bacterial biofilms.an enzyme-linked lectinsorbent assay (ella) was developed for quantification and characterization of extracellular polysaccharides produced by 1- and 4-day biofilms of 10 bacterial strains isolated from food industry premises. peroxidase-labeled concanavalin a (cona) and wheat germ agglutinin (wga) were used, as they specifically bind to saccharide residues most frequently encountered in biofilms matrices: d-glucose or d-mannose for cona and n-acetyl-d-glucosamine or n-acetylneuraminic acid for ...200010788349
limitation of adhesion and growth of listeria monocytogenes on stainless steel surfaces by staphylococcus sciuri biofilms.the adhesion and subsequent development of listeria monocytogenes on stainless steel was studied in the absence and in the presence of a staphylococcus sciuri biofilm. in the three growth media studied, the percentage of adherent cells was reduced to nearly the same extent by the presence of 1-day biofilms of staph. sciuri for the two strains of l. monocytogenes studied. one-day biofilms of staph. sciuri exhibited the same exopolysaccharide content per square centimetre, although they colonized ...200010792517
staphylococcus sciuri: recommendation for simple identification.we report the isolation of staphylococcus sciuri, primarily animal species, from samples taken from hospitalised patients. considering that staphylococcus sciuri often remains unrecognised in routine laboratory practice, we propose the criteria for simple identification of this bacterium.200010872689
identification of a plasmid-borne chloramphenicol-florfenicol resistance gene in staphylococcus sciuri.the 16.5-kbp plasmid pscfs1 from staphylococcus sciuri mediated combined resistance to chloramphenicol and florfenicol. the gene responsible for this resistance property, cfr, was cloned and sequenced. the amino acid sequence of the cfr protein revealed no homology to known acetyltransferases or efflux proteins involved in chloramphenicol and/or florfenicol resistance or to other proteins whose functions are known.200010952608
peritonitis due to staphylococcus sciuri in a patient on continuous ambulatory peritoneal dialysis.among the coagulase-negative staphylococci, staphylococcus sciuri has rarely been described as the aetiology of continuous ambulatory peritoneal dialysis (capd) peritonitis. it has been reported in 1 case of endocarditis and has been isolated from peritoneal dialysis fluid in 2 patients. the case reported here describes capd peritonitis due to s. sciuri shortly after a previous episode due to s. aureus, showing the necessity to identify coagulase-negative staphylococci to find new species that c ...200011200385
recruitment of the meca gene homologue of staphylococcus sciuri into a resistance determinant and expression of the resistant phenotype in staphylococcus aureus.strains of methicillin-resistant staphylococcus aureus (mrsa) have become the most important causative agents of hospital-acquired diseases worldwide. the genetic determinant of resistance, meca, is not a gene native to s. aureus but was acquired from an extraspecies source by an unknown mechanism. we recently identified a close homologue of this gene in isolates of staphylococcus sciuri, a taxonomically primitive staphylococcal species recovered most frequently from rodents and primitive mammal ...200111274099
possible virulence factors of staphylococcus sciuri.staphylococcus sciuri is an opportunistic pathogen of controversial clinical significance. the factors that contribute to colonization and/or infection caused by this bacterium have not been studied intensively so far. the present research was carried out in order to study the presence of potential virulence factors in 121 human and animal isolates of this bacterium. isolates were examined for biofilm formation, hemagglutination, presence of clumping factor, production of spreading factors and e ...200111356566
bacterial flora of the conjunctiva and nasal cavity in normal and diseased captive bustards.a survey was carried out to describe the normal aerobic bacterial flora of the conjunctiva and nasal cavity of captive houbara bustards (chlamydotis undulata), kori bustards (ardeotis kori), and white-bellied bustards (eupodotis senegalensis) maintained at the national avian research center, abu dhabi, united arab emirates. a total of 58 samples were examined from the nasal cavity and 55 samples from the conjunctiva of healthy bustards. there was no bacterial growth in 45% of conjunctival sample ...200111417826
staphylococcus sciuri as a part of skin, nasal and oral flora in healthy dogs.the coagulase-negative species staphylococcus sciuri is widespread in nature and is associated with a variety of domestic and wild animals. however, the occurrence of s. sciuri in dogs has received little attention so far. in the present study, we established the prevalence of s. sciuri in a large population of healthy dogs, and characterized isolated strains. samples from two mucous membrane sites (anterior nares and mouth), and two hair-coated sites (head and withers) were taken from 122 dogs ...200111423208
antibiotic resistance in coagulase-negative staphylococci isolated from cope's gray treefrogs (hyla chrysoscelis).organisms belonging to the genus staphylococcus were isolated on mannitol salt agar from the feces of wild caught cope's gray treefrogs (hyla chrysoscelis) from east-central kansas. all 222 presumptive isolates were confirmed as coagulase-negative staphylococci with staphylococcus sciuri and staphylococcus xylosus being most prevalent. antibiotic susceptibility patterns to five different antibiotics were determined and the results indicated 99% of all isolates were resistant to penicillin g and ...200111750814
intravenous catheter-related septic shock caused by staphylococcus sciuri and escherichia vulneris.staphylococcus sciuri and escherichia vulneris were isolated concurrently in a blood sample from a patient with septic shock, which was probably associated with an indwelling catheter. our results also showed that s. sciuri is an important reservoir of genetic determinants of beta-lactam resistance as a human pathogen carrying the meca and beta-lactamase genes.200111868769
transfer of microorganisms, including listeria monocytogenes, from various materials to beef.the quantity of microorganisms that may be transferred to a food that comes into contact with a contaminated surface depends on the density of microorganisms on the surface and on the attachment strengths of the microorganisms on the materials. we made repeated contacts between pieces of meat and various surfaces (stainless steel and conveyor belt materials [polyvinyl chloride and polyurethane]), which were conditioned with meat exudate and then were contaminated with listeria monocytogenes, sta ...200212147503
surgical wound infection associated with staphylococcus sciuri.we describe a case of surgical wound infection due to staphylococcus sciuri. the isolated strain was susceptible to trimethoprim-sulfamethoxazole, erythromycin, chloramphenicol, ciprofloxacin and vancomycin and resistant to gentamicin, clindamycin, rifampicin, methicillin, ampicillin and ceftriaxone. the multiresistance of the strain had a serious impact on the prolonged course of the infection. although this bacterium is principally found in animals, our strain was probably of nosocomial origin ...200212374362
staphylococcus sciuri gene erm(33), encoding inducible resistance to macrolides, lincosamides, and streptogramin b antibiotics, is a product of recombination between erm(c) and erm(a).a gene which mediates inducible resistance to macrolides, lincosamides, and streptogramin b antibiotics, designated erm(33), was detected on the staphylococcus sciuri plasmid pscfs1. analysis of the erm(33) reading frame suggested that this gene was the product of a recombination between an erm(c) gene and an erm(a) gene. such a recombination event is a novel observation for erm genes.200212384375
distribution of meca-harboring staphylococci in healthy mares.the prevalence of staphylococci that harbor the meca gene responsible for methicillin resistance was examined in healthy breeding mares. staphylococci often cause diseases of horses such as metritis, keratitis, and abscess. methicillin-resistant staphylococci would make antibiotic treatments ineffective, so it may be significant to know the distribution of meca-harboring staphylococci in mares. isolation of meca-harboring staphylococci was achieved from nares and pasterns of 100 mares in hokkaid ...200212399608
a comparison of biodegradation of phenol and homologous compounds by pseudomonas vesicularis and staphylococcus sciuri strains.pseudomonas vesicularis and staphylococcus sciuri were isolated as dominant strains from phenol-acclimated activated sludge. p. vesicularis was an efficient degrader of phenol, catechol, p-cresol, sodium benzoate and sodium salicylate in a single substrate system. under similar conditions s. sciuri degraded only phenol and catechol from among aromatic compounds that were tested. cell-free extracts of p. vesicularis grown on phenol (376 mg l(-1)), sodium benzoate (576 mg l(-1)) and sodium salicyl ...200212708825
molecular analysis of the plasmid-borne aaca/aphd resistance gene region of coagulase-negative staphylococci from chickens.the aim of this study was to analyse selected coagulase-negative staphylococci from chickens for the genetic basis of plasmid-borne resistance to gentamicin, tobramycin and kanamycin, and also for the presence of tn4001-like elements.200312716781
in vitro activity of 10 antimicrobial agents against bacteria isolated from cows with clinical mastitis.the susceptibility of 495 strains of bacteria, recently isolated in france from cows with clinical mastitis, to 10 antimicrobial agents--penicillin g, cloxacillin, oxacillin, cephalexin, cefazolin, cephapirin, cefquinome, neomycin, ampicillin and colistin--was determined by measuring their minimum inhibitory concentrations (mics). overall, the levels of resistance were very low except for staphylococci and penicillin g. the 167 streptococcal strains were susceptible to all of the beta-lactams te ...200312723630
a survey of antibiotic resistance in micrococcaceae isolated from italian dry fermented sausages.the transfer of bacteria that are resistant to antimicrobial agents or resistance genes from animals to humans via the food chain is increasingly a problem. therefore, it is important to determine the species and the numbers of bacteria involved in this phenomenon. for this purpose, 148 strains of microstaphylococci were isolated from three types of italian dry fermented sausages. eight of 148 strains belonged to the genera kocuria and micrococcus. the remaining 140 strains belonged to 11 differ ...200312800992
differentiation of staphylococcus sciuri strains isolated from free-living rodents and insectivores.twenty-nine staphylococcus sciuri strains isolated from free-living insectivores and rodents were comparatively analysed for their biochemical capacities and their smai macrorestriction patterns. the 29 s. sciuri isolates represented 21 different biotypes and 22 different smai macrorestriction types. this observation confirmed that s. sciuri isolates obtained from insectivores and rodents living in natural environments constituted a heterogeneous population. cluster analysis revealed that the ma ...200312864900
isolation of members of the staphylococcus sciuri group from urine and their relationship to urinary tract infections.during a 3-year study period, 32,741 urine samples were analyzed for the presence of members of the staphylococcus sciuri group (s. sciuri, s. lentus, and s. vitulinus), and 13 isolates were identified. they presented 0.79% of the total number of coagulase-negative staphylococci isolated. one case of symptomatic urinary tract infection and five possible cases of asymptomatic bacteriuria caused by these bacteria were established. it is noteworthy, however, that over 50% of the isolates originated ...200314605178
influence of the incubation atmosphere on the production of biofilm by staphylococci.biofilm formation by staphylococcus epidermidis, staphylococcus hemolyticus, staphylococcus sciuri and staphylococcus aureus in aerobic, anaerobic and co2 incubation atmospheres was quantified by the modified microtiter plate test. the s. epidermidis and s. aureus strains showed significantly lower biofilm production when grown in a co2-rich environment compared to that exhibited in aerobic incubation. the amount of biofilm produced by these strains under anaerobic conditions did not differ sign ...200314616685
development of methicillin resistance in clinical isolates of staphylococcus sciuri by transcriptional activation of the meca homologue native to s.the beta-lactam resistance gene meca was acquired by staphylococcus aureus from an extraspecies source. the search for the possible origin of this gene has led to the identification of a close structural homologue of meca as a native gene in the animal species staphylococcus sciuri. surprisingly, the overwhelming majority of s. sciuri isolates were fully susceptible to beta-lactam antibiotics in spite of the ubiquitous presence of the meca homologue in the bacteria. we now describe two unusual s ...200312511511
ecology of mixed biofilms subjected daily to a chlorinated alkaline solution: spatial distribution of bacterial species suggests a protective effect of one species to another.three bacterial strains (kocuria sp. c714.1, brevibacterium linens b337.1 and staphylococcus sciuri ccl101) were grown together on stainless steel and were subjected daily to a commercial alkaline chlorine solution (22 mg l-1 of free chlorine, ph 11) over a period of 4 weeks. after the daily chemical shock, culture madia [1:20 dilution of tryptic soy broth (tsb-ye/20) or diluted whey] was deposited on the biofilms. the chemical shocks led first to a drop in the culturable population, followed by ...200312542714
occurrence of resistance to antibiotics, metals, and plasmids in clinical strains of staphylococcus spp.presence of staphylococci resistant to antibiotics is of concern, as is the possible spread of resistance determinants among staphylococcus species. during the past several years, a series of staphylococcal isolates that demonstrated reduced susceptibility to vancomycin or other glycopeptides has been reported. however, there are no useful data on species sensitivity of coagulase-negative staphylococci to heavy metals.200312700009
isolation and molecular characterization of multiresistant staphylococcus sciuri and staphylococcus haemolyticus associated with skin and soft-tissue infections.the isolation, molecular identification and genotyping of multiresistant staphylococcus sciuri and staphylococcus haemolyticus from skin and soft-tissue infections are reported. accurate and full identification of three coagulase-negative staphylococcal isolates was achieved using pcr, while the api staph method failed to identify an isolate of s. haemolyticus fully. the pcr assay, which detects polymorphism in the 16s-23s rrna spacer region, is shown to be potentially useful for rapid and accur ...200414663105
impact of cleaning and disinfection agents on biofilm structure and on microbial transfer to a solid model food.to determine how single cells and microcolonies transfer to food from open surfaces in the meat industry.200415239692
the etiologic diagnosis of infectious discitis is improved by amplification-based dna analysis.blood cultures and cultures of disc material are required to identify and treat bacterial agents responsible for septic spondylodiscitis, but these methods have limited sensitivities. we undertook this study to compare nonculture amplification-based dna analysis with conventional culture of disc aspirate.200415457468
nucleotide sequence and organization of the multiresistance plasmid pscfs1 from staphylococcus sciuri.the multiresistance plasmid pscfs1 from staphylococcus sciuri was sequenced completely and analysed with regard to its gene organization and the putative role of a novel abc transporter in antimicrobial resistance.200415471995
phylogenetic analysis of bacterial populations in waters of the former texcoco lake, mexico.molecular techniques were used to compare the compositions of the bacterial communities of the 2 following lagoons from the former soda texcoco lake, mexico: the restored facultativa lagoon and the nabor carrillo lagoon. ribosomal intergenic spacer analysis (risa) revealed that bacterial communities of the 2 lagoons were different and presented a relatively low diversity. clone libraries of 16s rdna genes were constructed, and significant phylotypes were distinguished by restriction fragment len ...200415714236
identification of coagulase-negative staphylococci other than staphylococcus epidermidis by automated ribotyping.as routine identification of coagulase-negative staphylococci is problematic, the performance of automated ribotyping was evaluated for identification of coagulase-negative staphylococci other than staphylococcus epidermidis. in total, 177 isolates were tested, comprising 149 isolates from blood samples, 15 isolates that were not identified by internal transcribed spacer (its)-pcr in a previous study, and 13 reference strains. the identification results were compared with those obtained by the a ...200515715714
the pls gene found in methicillin-resistant staphylococcus aureus strains is common in clinical isolates of staphylococcus sciuri.pls, a gene found in type i staphylococcal cassette chromosome mec (sccmec) regions of methicillin-resistant staphylococcus aureus strains, was present in 12 of the 15 human clinical staphylococcus sciuri isolates studied. pls was expressed in the s. sciuri isolates, although at a lower level than in s. aureus. other parts of sccmec could also be found in the s. sciuri genome.200515750121
isolation and molecular characterization of staphylococcus sciuri in the hospital environment.staphylococcus sciuri is a principally animal-associated bacterial species, but its clinical relevance for humans is increasing. our study aimed to provide the first insight into the prevalence of this bacterium in a hospital environment. a 3-month surveillance was conducted in a hospital located in belgrade, serbia, and 1,028 samples taken from hands of medical personnel, medical devices, and various hospital surfaces were screened for s. sciuri presence. in total, 108 isolates were obtained, w ...200515956397
staphylococcus sciuri: an unusual cause of pelvic inflammatory disease.we present the case of polymicrobial pelvic inflammatory disease (pid) that involved staphylococcus sciuri, s. epidermidis, and streptococcus agalactiae. in order to determine the frequency of s. sciuri isolation from the female lower genital tract, 3415 vaginal samples were analysed during the one-year study period. s. sciuri was isolated from three (0.09%) samples. in all the three cases, s. sciuri was obtained in mixed culture from outpatients without symptoms of infection. while the origin o ...200515969784
a comparative evaluation of phenotypic and molecular methods in the identification of members of the staphylococcus sciuri group.members of the staphylococcus sciuri group (s. sciuri, s. lentus, and s. vitulinus) are coagulase-negative, novobiocin-resistant staphylococci that could be distinguished from other staphylococci on the basis of positive oxidase activity. in the present study, a scheme based on conventional methods and utilization of various carbohydrates was evaluated for the identification of oxidase-positive staphylococci, and validated using two molecular techniques. of the 173 oxidase-positive staphylococca ...200515997708
plasmid-specified femabx-like immunity factor in staphylococcus sciuri dd 4747.a plasmid from staphylococcus sciuri dd 4747 had three open reading frames: a replication gene, an n-acetylmuramyl-l-alanine amidase-like gene, and a gene similar to the lysostaphin endopeptidase resistance gene (epr/lif). the epr-like gene was introduced into s. aureus rn4220; the recombinant strain was more resistant to lysostaphin endopeptidase and its cell wall peptidoglycan contained more serines and fewer glycines than the parental strain with the shuttle vector alone. based on both its fu ...200516006076
a new mechanism for chloramphenicol, florfenicol and clindamycin resistance: methylation of 23s ribosomal rna at a2503.the gene product of cfr from staphylococcus sciuri confers resistance to chloramphenicol, florfenicol and clindamycin in staphylococcus spp. and escherichia coli. cfr is not similar to any other known chloramphenicol resistance determinant. comparative investigation of e. coli with and without a plasmid-encoded cfr showed a decreased drug binding to ribosomes in the presence of cfr. as chloramphenicol/florfenicol and clindamycin have partly overlapping drug binding sites on the ribosome, the mos ...200516091044
survey of genes encoding staphylococcal enterotoxins, toxic shock syndrome toxin 1, and exfoliative toxins in members of the staphylococcus sciuri group.genes encoding staphylococcal enterotoxins (sea to see, seg, and seh), toxic shock syndrome toxin 1 (tst), and exfoliative toxins (eta and etb) were not detected in a large panel of 48 staphylococcus sciuri group isolates tested. this strongly suggests that production of the staphylococcal exotoxins by these bacteria is highly unlikely.200516145164
high-level (beta)-lactam resistance and cell wall synthesis catalyzed by the meca homologue of staphylococcus sciuri introduced into staphylococcus aureus.a close homologue of meca, the determinant of broad-spectrum beta-lactam resistance in staphylococcus aureus was recently identified as a native gene in the animal commensal species staphylococcus sciuri. introduction of the meca homologue from a methicillin-resistant strain of s. sciuri into a susceptible strain of s. aureus caused an increase in drug resistance and allowed continued growth and cell wall synthesis of the bacteria in the presence of high concentrations of antibiotic. we determin ...200516166526
genetic analysis of mec a homologues in staphylococcus sciuri strains derived from mastitis in dairy cattle.methicillin-resistant staphylococcus aureus (mrsa) is defined by the presence of the mec a gene, which is considered to have been transferred horizontally from unknown bacterial species to s. aureus. as a candidate of evolutionary precursor of the mec a, the mec a-like gene (mec a homologue), which is ubiquitously present in staphylococcus sciuri has been proposed. in this study, sequences of the mec a homologue in four s. sciuri strains (scbm 1-scbm 4) derived from dairy cows were determined to ...200516201922
expression of high-level methicillin resistance in staphylococcus aureus from the staphylococcus sciuri mec a homologue: role of mutation(s) in the genetic background and in the coding region of mec a.a close homologue of the mec a gene, the primary drug resistance determinant in methicillin resistant staphylococcus aureus (mrsa), is ubiquitous in the animal commensal species staphylococcus sciuri, yet most isolates of this staphylococcal species are susceptible to beta-lactam antibiotics including methicillin. recently, we showed that in a methicillin-resistant mutant of s. sciuri prepared in the laboratory, the mec a homologue is converted to an antibiotic resistance gene by a point mutatio ...200516201923
identification and characterization of clinical isolates of members of the staphylococcus sciuri group.a total of 28 staphylococcal isolates from human clinical specimens belonging to the staphylococcus sciuri group were identified and characterized. the api staph and id32 staph correctly identified s. sciuri and s. lentus but not s. vitulinus strains. identification to the subspecies level was possible only by a pcr-based method.200515695717
[effects of pine wood nematode on propagation of its carrying bacteria].in this paper, the aseptic eggs of bursaphelenchus xylophilus were obtained after treated with 30% h202, and cultured with pinus thunbergii callus. ten b. xylophilus-carrying bacterial strains directly isolated from diseased p. thugbergii and p. massoniana in six epidemic provinces i.e., gcm6-2a pseudomonas putida, gcm6-1a p. putida, zpb1-2a p. putida, hem2a pseudomonas sp., hem1a pseudomonas sp., hem142b pseudomonas sp., gcm1-3a p. cepacia and hm3 pantoeu sp., zpb4-2b staphylococcus sciuri and ...200516515211
evaluation of phenotypic and molecular methods for detection of oxacillin resistance in members of the staphylococcus sciuri group.in this paper we report on an experimental evaluation of phenotypic and molecular methods as means for the detection of oxacillin resistance in members of the staphylococcus sciuri group. a total of 109 s. sciuri group member isolates (92 s. sciuri isolates, 9 s. lentus isolates, and 8 s. vitulinus isolates) were tested by the disk diffusion method, the agar dilution method, the oxacillin salt-agar screening method, slide latex agglutination for pbp 2a, and pcr assay for meca as the reference me ...200616517879
distribution of florfenicol resistance genes fexa and cfr among chloramphenicol-resistant staphylococcus isolates.a total of 302 chloramphenicol-resistant staphylococcus isolates were screened for the presence of the florfenicol/chloramphenicol resistance genes fexa and cfr and their localization on mobile genetic elements. of the 114 isolates from humans, only a single staphylococcus aureus isolate showed an elevated mic to florfenicol, but did not carry either of the known resistance genes, cfr or fexa. in contrast, 11 of the 188 staphylococci from animal sources were considered florfenicol resistant and ...200616569824
construction and analysis of fractional multifactorial designs to study attachment strength and transfer of listeria monocytogenes from pure or mixed biofilms after contact with a solid model food.the aim of this study was to establish which of seven factors influence the adhesion strength and hence bacterial transfer between biofilms containing listeria monocytogenes (pure and two-species biofilms) and tryptone soya agar (tsa) as a solid organic surface. the two-species biofilms were made of l. monocytogenes and one of the following species of bacteria: the nonpathogenic organisms kocuria varians, pseudomonas fluorescens, and staphylococcus sciuri and ccl 63, an unidentified gram-negativ ...200616597925
resistance to macrolides, lincosamides, streptogramins, and linezolid among members of the staphylococcus sciuri group.this study aimed to characterize the resistance profiles of the staphylococcus sciuri group members to macrolides, lincosamides, streptogramins (mls antibiotics), and linezolid upon analysis of large series of isolates that included 162 s. sciuri isolates, nine s. lentus, and one s. vitulinus. the evaluation of their susceptibility by disk diffusion and agar dilution methods, along with pcr detection of the resistance genes erm(a), erm(b), erm(c), mef(a), lnu(a), and lnu(b), were performed. resi ...200616922627
[catheter associated staphylococcus sciuri sepsis in a patient with acute myeloid leukemia].the coagulase-negative bacterial species staphylococcus sciuri is widely distributed in the natural environment. although principally found in animals, s. sciuri is occasionally isolated from human samples. in this paper, s. sciuri bacteremia which was associated with an indwelling catheter of a patient with acute myeloid leukemia (aml) and neutropenia was presented. an empirical intravenous antibiotic therapy (meropenem, vancomycin) was initiated with the preliminary diagnosis of febrile neutro ...200617205699
the prevalence of methicillin-resistant staphylococci in healthy horses in the netherlands.two hundred healthy horses housed at 23 different farms and one clinic and 42 persons in close contact with these horses were screened for the presence of methicillin resistant staphylococci. samples were taken from the nose and the pastern of the horses and from the nose and throat of the humans and incubated in selective media. isolates were identified by standard techniques and their susceptibilities were tested using an agar diffusion method. methicillin-resistant strains were tested for the ...200616303264
biochemical evidence for the proteolytic degradation of infectious prion protein prpsc in hamster brain homogenates by foodborne bacteria.prp(sc) is a general term to describe the infectious agent causing transmissible spongiform encephalopathy (tse), and the protease-resistant form of cellular prp(c). in this study, we have identified several protease-secreting bacteria able to degrade prp(sc) under more or less native conditions (30 degrees c, ph 8), focusing on strains isolated mainly from cheese. one hundred and ninty-nine protease-secreting isolates belonging to the actinomycetales and bacillales were screened for the express ...200616464698
supplementary biochemical tests useful for the differentiation of oxidase positive staphylococci.differentiation of the oxidase positive staphylococci, staphylococcus sciuri, staphylococcus lentus, staphylococcus vitulinus and staphylococcus fleurettii, based on tributyrin, urease, caseinase, gelatinase and dnase activity is described. these tests may be used for preliminary identification of oxidase positive isolates of staphylococci resulting in more accurate identification of these species.200717207599
a highly pathogenic strain of staphylococcus sciuri caused fatal exudative epidermitis in piglets.staphylococcus sciuri are important human pathogens responsible for endocarditis, peritonitis, septic shock, urinary tract infection, pelvic inflammatory disease and wound infections. however, little information is known regarding the pathogenicity of s. sciuri to animals. from the pericardial fluid of a diseased piglet with exudative epidermitis (ee), we isolated a strain of staphylococcus in pure culture. surprisingly, this isolate was a member of s. sciuri rather than s. hyicus as identified ...200717215958
tetracycline resistance and distribution of tet genes in members of the staphylococcus sciuri group isolated from humans, animals and different environmental sources. 200717229556
shared functional attributes between the meca gene product of staphylococcus sciuri and penicillin-binding protein 2a of methicillin-resistant staphylococcus aureus.the genome of staphylococcus aureus is constantly in a state of flux, acquiring genes that enable the bacterium to maintain resistance in the face of antibiotic pressure. the acquisition of the meca gene from an unknown origin imparted s. aureus with broad resistance to beta-lactam antibiotics, with the resultant strain designated as methicillin-resistant s. aureus (mrsa). epidemiological and genetic evidence suggests that the gene encoding pbp 2a of mrsa might have originated from staphylococcu ...200717567045
aminoglycoside resistance in members of the staphylococcus sciuri group.this study investigated the prevalence of aminoglycoside resistance and genes encoding aminoglycoside-modifying enzymes in members of the staphylococcus sciuri group. a total of 304 s. sciuri group member isolates (284 s. sciuri, 12 s. lentus, and 8 s. vitulinus) from humans (n = 34), animals (n = 133), and environmental sources (n = 137; out-hospital and hospital environment, food) were examined for their susceptibility to amikacin, gentamicin, isepamicin, kanamycin, neomycin, netilmicin, sisom ...200717650957
evolution of slime production by coagulase-negative staphylococci and enterotoxigenic characteristics of staphylococcus aureus strains isolated from various human clinical specimens.the present study was designed to determine the slime production of coagulase-negative staphylococci (cons) and the enterotoxigenic properties of staphylococcus aureus strains, and to evaluate the clinical importance of slime-producing cons and enterotoxigenic s. aureus strains isolated from various human clinical specimens. for this purpose, a total of 120 staphylococcus strains were isolated and identified, and further characterized for their slime production and enterotoxigenicity. of the cli ...200717893164
distribution of staphylococcal cassette chromosome mec (sccmec) types i, ii, iii and iv in coagulase-negative staphylococci from patients attending a tertiary hospital in southern brazil.coagulase-negative staphylococci (cons) are now recognized as the aetiological agents of an important range of infections in humans. most developed countries have reported an increase in cons infections in hospitalized patients that are resistant to meticillin and other antibiotics. staphylococcal cassette chromosome mec (sccmec) typing is essential for understanding the molecular epidemiology of meticillin-resistant staphylococcus strains. sccmec elements are currently classified into types i t ...200717893169
cleaning and handling implements as potential reservoirs for bacterial contamination of some ready-to-eat foods in retail delicatessen environments.this study assessed the association of bacteria with cleaning tools, such as floor mops (n = 25) and cleaning cloths (n = 39), and handling devices, such as disposable plastic gloves (n = 20), used during filled baguette and assorted salad preparation in four selected retail delicatessens in johannesburg, south africa. samples of each cleaning or handling tool were prepared for aerobic (apc), coliform (cc), escherichia coli (ec), bacillus cereus (bcc), and staphylococcus aureus (sac) counts, as ...200718095448
species distribution and properties of staphylococci from canine dermatitis.the occurrence and phenotypic, and genotypic properties of 24 staphylococcus isolates from canine dermatitis were investigated. the predominant staphylococcal species was staphylococcus intermedius. the other species such as staphylococcus chromogenes, staphylococcus sciuri, staphylococcus aureus, staphylococcus saprophyticus, staphylococcus epidermidis, and staphylococcus capitis were only occasionally isolated. the study showed low level biochemical diversity among s. intermedius isolates. res ...200717126372
is21-558 insertion sequences are involved in the mobility of the multiresistance gene cfr.during a study of florfenicol-resistant porcine staphylococci from denmark, the genes cfr and fexa were detected in the chromosomal dna or on plasmids of staphylococcus hyicus, staphylococcus warneri, and staphylococcus simulans. a novel variant of the phenicol resistance transposon tn558 was detected on the ca. 43-kb plasmid pscfs6 in s. warneri and s. simulans isolates. sequence analysis of a 22,010-bp segment revealed that the new tn558 variant harbored an additional resistance gene region in ...200717145796
[a case report of staphylococcus sciuri scalded skin syndrome]. 200818433570
monitoring and identification of bacteria associated with safety concerns in the manufacture of são jorge, a portuguese traditional cheese from raw cow's milk.the aim of this study was to assess the hygienic quality of raw milk used in the manufacture of são jorge, a protected denomination of origin portuguese semihard cheese, as well as to ascertain the sanitary conditions prevailing during its processing. viable counts of enterobacteriaceae and micrococcaceae were accordingly obtained, pertaining to 21 independent batches (including samples of raw milk, curd, and cheeses after 1, 3, and 4 months of ripening), from 7 dairy farms. standard plate count ...200818522034
antibiotic resistance genes and identification of staphylococci collected from the production chain of swine meat commodities.staphylococci harbouring antibiotic resistance (ar) genes may represent a hazard for human health and, as other resistant food-related bacteria, they contribute to the spread of ar. in this study, we isolated resistant staphylococci from an entire swine production chain and investigated the occurrence of 11 genes [aac(6')ie-aph(2'')ia, blaz, meca, vana, vanb, erma, ermb, ermc, tet(m), tet(o) and tet(k)] encoding resistance to some antibiotics largely used in clinical practice. the 66 resistant s ...200817993395
penicillin-binding proteins and cell wall composition in beta-lactam-sensitive and -resistant strains of staphylococcus sciuri.a close homologue of the acquired staphylococcus aureus meca gene is present as a native gene in staphylococcus sciuri. we determined the patterns of penicillin-binding proteins (pbps) and the peptidoglycan compositions of several s. sciuri strains to explore the functions of this meca homologue, named pbpd, in its native s. sciuri environment. the protein product of pbpd was identified as pbp4 with a molecular mass of 84 kda, one of the six pbps present in representatives of each of three subsp ...200818024515
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