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dyadobacter fermentans gen. nov., sp. nov., a novel gram-negative bacterium isolated from surface-sterilized zea mays stems.a gram-negative bacterium, designated ns114t, was isolated from duplicate treatments of surface-sterilized zea mays stems. the plants were grown in synthetic soil under greenhouse conditions and watered with fertilizer containing no nitrogen. strain ns114t could not be isolated from plants watered with the standard level or 20% (w/v) of the standard level of nitrogen. cells occurred as pairs in young cultures that attached to form angled arrangements in r2a broth and occasionally formed rounded, ...200010758885
polyamine distribution profiles in the eighteen genera phylogenetically located within the flavobacterium-flexibacter-cytophaga complex.cellular polyamines of eighteen genera belonging to the flavobacterium-flexibacter-cytophaga complex were analysed by ion exchange liquid chromatography. homospermidine was the major polyamine in the genera bergeyella, riemerella, ornithobacterium, weeksella, capnocytophaga, polaribacter and psychroflexus belonging to the family flavobacteriaceae. in the family spirosomaceae, runella, spirosoma and flectobacillus species contained spermidine whereas cyclobacterium species contained homospermidin ...200111491527
viable cytophaga-like bacterium in the 0.2 microm-filtrate seawater.a strain of the cytophaga-like bacterium (clb), nano-1, was isolated from the 0.2 microm-filtrate of natural seawater. both cellular fatty acid and 16s rdna sequence analyses indicated that nano-1 is closely affiliated to the marine gliding clb genus, microscilla. nano-1 was observed to undergo cyclic morphological change typical of the genus microscilla, and sub-0.2-microm cells were formed in the late stationary phase. the sub-0.2-microm cells were repeatedly revived and subcultured. formation ...200111876369
sequence analysis of a 101-kilobase plasmid required for agar degradation by a microscilla isolate.an agar-degrading marine bacterium identified as a microscilla species was isolated from coastal california marine sediment. this organism harbored a single 101-kb circular dna plasmid designated psd15. the complete nucleotide sequence of psd15 was obtained, and sequence analysis indicated a number of genes putatively encoding a variety of enzymes involved in polysaccharide utilization. the most striking feature was the occurrence of five putative agarase genes. loss of the plasmid, which occurr ...200111722934
phylogenetic structure of the genera flexibacter, flexithrix, and microscilla deduced from 16s rrna sequence analysis.the 16s rdna sequences of 40 strains of 17 species in the genus flexibacter, 5 strains of 4 species in the genus microscilla, and 1 strain of flexithrix dorotheae, including all type strains of approved and validated species in these genera, were determined to reveal their phylogenetic relationships. the 16s rrna sequence analysis demonstrated the extreme heterogeneity of the genera flexibacter and microscilla. the strains examined diverged into 24 distinct lines of descent (1 group included bot ...200212469298
algoriphagus ratkowskyi gen. nov., sp. nov., brumimicrobium glaciale gen. nov., sp. nov., cryomorpha ignava gen. nov., sp. nov. and crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats.several cold-adapted strains isolated from a variety of algal-rich antarctic and southern ocean samples formed three distinct groups within the class flavobacteria, phylogenetically distant from other cultivated species. the first taxon, designated algoriphagus ratkowskyi gen. nov., sp. nov., was isolated from sea ice and from saline lake cyanobacterial mats and includes non-motile, strictly aerobic, saccharolytic rod-like or serpentine strains that were most closely related to the genus cycloba ...200313130017
distribution of two triamines, spermidine and homospermidine, and an aromatic amine, 2-phenylethylamine, within the phylum bacteroidetes.cellular polyamines of the newly additional 19 species belonging to the class bacteroides of the phylum bacteroidetes were analyzed by hplc to display polyamine distribution as a chemotaxonomic marker within the total 41 species. three profiles, the presence of spermidine, the presence of homospermidine and the absence of both triamines, corresponded to their phylogenetical positions within the four families of the class. the occurrence of an aromatic amine, 2-phenylethylamine, extracted into ce ...200415747230
emendation of the genus flammeovirga and flammeovirga aprica with the proposal of flammeovirga arenaria nom. rev., comb. nov. and flammeovirga yaeyamensis sp. nov.the taxonomic positions of five bacterial strains isolated from the yaeyama islands of japan and 'microscilla arenaria' nbrc 15982 were determined using a polyphasic taxonomic approach. 16s rrna gene sequence analyses placed all of the strains close to the genus flammeovirga. dna-dna hybridization studies, biochemical and physiological characterizations and chemotaxonomic analyses suggested that 'm. arenaria' nbrc 15982 and the five novel isolates represented two separate species of the genus fl ...200616957105
heme uptake by microscilla marina and evidence for heme uptake systems in the genomes of diverse marine bacteria.the ability to acquire diverse and abundant forms of iron would be expected to confer a survival advantage in the marine environment, where iron is scarce. marine bacteria are known to use siderophores and inorganic iron, but their ability to use heme, an abundant intracellular iron form, has only been examined preliminarily. microscilla marina, a cultured relative of a bacterial group frequently found on marine particulates, was used as a model organism to examine heme uptake. searches of the g ...200818757577
marinoscillum gen. nov., a member of the family 'flexibacteraceae', with marinoscillum pacificum sp. nov. from a marine sponge and marinoscillum furvescens nom. rev., comb. nov.a novel strain, designated mrn461(t), was isolated from a marine sponge in micronesia. the 16s rrna gene sequence of the isolate showed 95.6 % similarity with that of 'microscilla furvescens' ifo (now nbrc) 15994. phylogenetic analysis revealed that the isolate and 'microscilla furvescens' ifo 15994 formed a distinct phyletic line within the family 'flexibacteraceae'. cells of strain mrn461(t) were gram-negative, long filamentous rods, motile by gliding. growth was observed at 15-40 degrees c (o ...200919406820
reclassification of flexibacter tractuosus (lewin 1969) leadbetter 1974 and 'microscilla sericea' lewin 1969 in the genus marivirga gen. nov. as marivirga tractuosa comb. nov. and marivirga sericea nom. rev., comb. nov.the taxonomic position of the misclassified strains [flexibacter] tractuosus kctc 2958t and '[microscilla] sericea' lmg 13021 was studied using a polyphasic approach. the two strains shared 99.1% 16s rrna gene sequence similarity and 28% dna-dna relatedness. on the basis of the phylogenetic evidence supported by genotypic and phenotypic data [flexibacter] tractuosus kctc 2958t and '[microscilla] sericea' lmg 13021 are classified as two distinct species in a novel genus, marivirga, in the family ...201019767357
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