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evaluation of pasco mic-id system for identifying gram-negative bacilli.a production model of the semi-automated pasco mic-id system (pasco laboratories, wheat ridge, colo.) was evaluated with 122 groups or species of gram-negative bacilli, which included typical (499 cultures) and atypical (37 cultures) strains of fermenters and nonfermenters. the pasco system identified 90.9% of 536 cultures accurately; these included 90.8% of 152 nonfermenters, 93.8% of 308 enteric fermenters, and 78.9% of 76 oxidase-positive fermenters. these results were obtained with the aid o ...19873323230
incidence of vaginal weeksella virosa (formerly group iif).the antimicrobial susceptibilities, biochemical properties, and cultural characteristics of six strains of weeksella virosa (formerly group iif) were determined. the main characteristics of this non-fermentative organism were production of cytochrome oxidase, gelatinase, and indole, but a lack of saccharolytic activity. a study was then made of the isolation rates of weeksella virosa from high vaginal swabs from 300 women: a healthy control group (n = 100), a general group with symptoms of vagin ...19883350982
isolation of weeksella virosa (formerly cdc group iif) from a vaginal sample. 19892504603
isolation of weeksella zoohelcum in septicaemia. 19892569133
the taxonomic relationship of certain environmental flavobacteria to the genus weeksella.forty environmental strains and reference cultures of flavobacterium, cytophaga and weeksella spp. were examined by numerical taxonomy. twenty-seven strains were recovered in four phena. phena 1a and 1b comprised 48% of the strains and were sufficiently similar to the genus weeksella as to suggest possible inclusion in this genus. they could not be accommodated in the existing species w. virosa and w. zoohelcum. strains from phenon 2 appear to belong neither in the flavobacterium or the weeksell ...19892592292
microbiological characteristics of weeksella virosa (formerly cdc group iif) isolated from the human genitourinary tract.weeksella virosa (formerly cdc group iif) is a nonsaccharolytic, oxidase- and catalase-positive, gram-negative rod which is unable to grow on macconkey agar. at 48 h of incubation on blood or chocolate agar, the colonies present a characteristic appearance: intensely mucoid, adherent, and cream colored as a result of the production of a nondiffusible yellow pigment. this microorganism has been isolated predominantly from the female genitourinary tract, which indicates the opportunity for sexual ...19902229364
response of weeksella virosa peritonitis to imipenem/cilastin.capd peritonitis is most commonly due to gram positive infection. gram negative bacillary infection is less frequent but is often seen in hospitalized patients or in those on antibiotics. weeksella virosa (formerly known as flavobacterium ii f) has been isolated from the vaginal secretions and urine of normal women. as gram negative colonization typically proceeds from the perineal region, weeksella virosa peritonitis might be expected in women at risk for gram negative peritonitis. a 33-year-ol ...19911680409
leg abscess caused by weeksella zoohelcum following a dog bite. 19921600024
a partial phylogenetic analysis of the "flavobacter-bacteroides" phylum: basis for taxonomic restructuring.on the basis of small subunit rrna sequence analyses five major subgroups within the flavobacteria-bacteroides phylum have been defined. these are tentatively designated the cytophaga subgroup (comprising largely cytophaga species), the flavobacter subgroup (comprising the true flavobacteria and the polyphyletic genus weeksella), the bacteroides subgroup (comprising the bacteroides and certain cytophaga-like bacteria), the sphingobacter subgroup (which contains the known sphingolipid-producing m ...199211541229
riemerella anatipestifer gen. nov., comb. nov., the causative agent of septicemia anserum exsudativa, and its phylogenetic affiliation within the flavobacterium-cytophaga rrna homology group.the phylogenetic position of the causative agent of septicemia anserum exsudativa, now most often referred to as [moraxella] anatipestifer (brackets indicate a generically misnamed taxon) or "[pasteurella] anatipestifer," was established by performing rrna cistron similarity studies. [moraxella] anatipestifer belongs to rrna superfamily v, together with the genera flavobacterium, cytophaga, flexibacter, weeksella, capnocytophaga, and sphingobacterium. the detailed structure of rrna superfamily v ...19938240957
a transposon-like sequence adjacent to the acci restriction-modification operon.we have cloned and sequenced the accirm genes from weeksella zoohelcum (the original identification of this strain as acinetobacter calcoaceticus was incorrect). our sequence differs in the coding regions from a previously published sequence by the addition of three nucleotides near the 3' end of the dna methyltransferase-encoding gene (accim). we have sequenced approx. 3 kb beyond this operon. two genes were found, convergently transcribed with the r-m operon. the first of these genes encodes a ...19957607529
phenotypic characteristics, fatty acid composition, and isoprenoid quinone content of cdc group iig bacteria.eleven strains of eugonic, nonoxidative, gram-negative rods isolated from clinical specimens formed a distinct group that was designated cdc group iig. five of the 11 isolates were from wounds. the phenotypic characteristics of cdc group iig were most similar to those of weeksella species, with the major difference being that cdc group iig strains grew on macconkey agar in 1 to 2 days, did not hydrolyze gelatin, and did not produce urease. all 11 strains of cdc group iig possessed a distinct fat ...19957751393
in vitro activities of quinolones, beta-lactams, tobramycin, and trimethoprim-sulfamethoxazole against nonfermentative gram-negative bacilli.from 1991 to 1995, 8,975 nonfermentative gram-negative bacilli were isolated from patients at the ohio state university medical center: 71% pseudomonas aeruginosa, 14% stenotrophomonas maltophilia, 7.6% acinetobacter baumannii, and < 2% each of 25 other species. the mics of trovafloxacin (cp-99,219), ciprofloxacin, ofloxacin, ampicillin-sulbactam, piperacillin, piperacillin-tazobactam, ceftazidime, cefoperazone, ceftriaxone, imipenem, tobramycin, and trimethoprim-sulfamethoxazole (tmp-smz) were ...19968726011
activities of hmr 3004 (ru 64004) and hmr 3647 (ru 66647) compared to those of erythromycin, azithromycin, clarithromycin, roxithromycin, and eight other antimicrobial agents against unusual aerobic and anaerobic human and animal bite pathogens isolated from skin and soft tissue infections in humans.the activities of hmr 3004 and hmr 3647 and comparator agents, especially macrolides, were determined by the agar dilution method against 262 aerobic and 120 anaerobic strains isolated from skin and soft tissue infections associated with human and animal bite wounds. hmr 3004 and hmr 3647 were active against almost all aerobic and fastidious facultative isolates (mic at which 90% of the isolates are inhibited [mic90], < or = 0.5 and 1 microg/ml, respectively) and against all anaerobes [bacteroid ...19989593139
the incidence of weeksella- and bergeyella-like bacteria in the food environment.eighty-five catalase- and oxidase-positive gram-negative rods and cocci susceptible to penicillin g were isolated from a variety of food sources. the phenotypic relationships of these isolates with reference cultures of bergeyella-like, chryseobacterium, empedobacter, myroides, moraxella, sphingobacterium and weeksella-like strains were examined by numerical taxonomy. seventy-three isolates were recovered in five groups; 80% of the isolates clustered in groups 1, 2 and 3 and produced indole, bea ...19989721639
a case of spontaneous peritonitis caused by weeksella virosa.a case of spontaneous peritonitis caused by weeksella virosa is reported. this flavobacterium has never been reported as a cause of spontaneous bacterial peritonitis. the patient responded to antimicrobial therapy. clinical and therapeutic implications are discussed.19989831416
linezolid activity compared to those of selected macrolides and other agents against aerobic and anaerobic pathogens isolated from soft tissue bite infections in humans.linezolid was tested against 420 aerobes and anaerobes, including 148 pasteurella isolates, by an agar dilution method. linezolid was active against all pasteurella multocida subsp. multocida and p. multocida subsp. septica isolates and most pasteurella canis, pasteurella dagmatis, and pasteurella stomatis isolates. the mic was </=2 microg/ml for staphylococci, streptococci, ef-4b, weeksella zoohelcum, fusobacterium nucleatum, other fusobacteria, porphyromonas spp., prevotella spp., peptostrepto ...199910348773
activity of gatifloxacin compared to those of five other quinolones versus aerobic and anaerobic isolates from skin and soft tissue samples of human and animal bite wound infections.the activity of gatifloxacin against 308 aerobes and 112 anaerobes isolated from bite wound infections was studied. gatifloxacin was active at </=0.016 microg/ml against all 148 pasteurella isolates (eight species and three subspecies) tested and all other aerobes tested, including actinobacillus-haemophilus spp., eikenella corrodens, neisseria weaveri, weeksella zoohelcum, staphylococci, and streptococci. fusobacteria were sometimes resistant. gatifloxacin mics at which 90% of the isolates were ...199910348774
polymicrobial tenosynovitis with pasteurella multocida and other gram negative bacilli after a siberian tiger bite.mammalian bites present a considerable clinical problem because they are often associated with bacterial infections. pasteurella multocida is a microorganism that commonly infects both canine and small feline bites. zoonotic infections developing after large feline bites have been recognised, although their reports are limited. we describe a 35 year old man who was bitten by a siberian tiger and who developed infectious tenosynovitis secondary to p multocida, bergeyella (weeksella) zoohelcum, an ...200011127273
oral colonization of aerobic and facultatively anaerobic gram-negative rods and cocci in irradiated, dentate, xerostomic individuals.this study aimed at investigating the oral colonization of aerobic and facultatively anaerobic gram-negative rods and cocci in head- and neck-irradiated, dentate, xerostomic individuals. subjects were recruited from a nasopharyngeal carcinoma clinic and were segregated into group a: <60 years (n=25, 48+/-6 years, 5+/-5 years post-irradiation) and group b: >or=60 years (n=8, 67+/-4 years, 2+/-2 years post-irradiation) and were compared with age- and sex-matched normal individuals, group c: <60 ye ...200111169132
polyamine distribution profiles in the eighteen genera phylogenetically located within the flavobacterium-flexibacter-cytophaga complex.cellular polyamines of eighteen genera belonging to the flavobacterium-flexibacter-cytophaga complex were analysed by ion exchange liquid chromatography. homospermidine was the major polyamine in the genera bergeyella, riemerella, ornithobacterium, weeksella, capnocytophaga, polaribacter and psychroflexus belonging to the family flavobacteriaceae. in the family spirosomaceae, runella, spirosoma and flectobacillus species contained spermidine whereas cyclobacterium species contained homospermidin ...200111491527
bergeyella (weeksella) zoohelcum associated with respiratory disease in a cat. 200212403523
isolation of a fastidious bergeyella species associated with cellulitis after a cat bite and a phylogenetic comparison with bergeyella zoohelcum strains.bergeyella zoohelcum is an uncommon zoonotic pathogen typically associated with cat or dog bites. previously, only five cases of b. zoohelcum infection have been reported. we report the isolation and characterization of a fastidious bergeyella species from acute cellulitis in the upper extremity of a 60-year-old woman. the organism was too fastidious for identification and susceptibility testing with traditional culture methods. the isolate was characterized further by pcr amplification and sequ ...200414715767
proposal to transfer 'aegyptianella ranarum', an intracellular bacterium of frog red blood cells, to the family flavobacteriaceae as 'candidatus hemobacterium ranarum' comb. nov.'aegyptianella ranarum' (order rickettsiales), an ultrastructurally defined small, gram-negative rod, is known to replicate in the red blood cells of frogs. heretofore, this bacterium has not been characterized genetically. we cloned and sequenced the 16s rrna (1310 bp) and gyrb (718 bp) genes of 'a. ranarum' from a canadian frog blood specimen. in situ hybridization (with an 'a. ranarum' 16s rrna gene polymerase chain reaction product as probe) and electron microscopy confirmed that 'a. ranarum ...200415142244
['in vitro' activity of different antimicrobial agents on gram-negative nonfermentative bacilli, excluding pseudomonas aeruginosa and acinetobacter spp].gram-negative nonfermentative bacilli (nfb) are widely spread in the environment. besides of difficulties for identification, they often have a marked multiresistance to antimicrobial agents, including those active against pseudomonas aeruginosa. the objective of this study was to evaluate the 'in vitro' activity of different antimicrobial agents on 177 gram-negative nonfermentative bacilli isolates (excluding pseudomonas aeruginosa and acinetobacter spp.) isolated from clinical specimens. minim ...200515991478
evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative rods.to evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ...200716822636
resistance in gram-negative bacilli in a cardiac intensive care unit in india: risk factors and outcome.the objective of this study was to compare the risk factors and outcome of patients with preexisting resistant gram-negative bacilli (gnb) with those who develop sensitive gnb in the cardiac intensive care unit (icu). of the 3161 patients ( n=3,161) admitted to the icu during the study period, 130 (4.11%) developed health care-associated infections (hais) with gnb and were included in the cohort study. pseudomonas aeruginosa (37.8%) was the most common organism isolated followed by klebsiella sp ...200818182755
lymphoepithelioma-like carcinoma of the urinary bladder in a cow associated with bovine papillomavirus type-2.lymphoepithelioma-like carcinoma (lelca) of the urinary bladder is reported in a 7-year-old cow that had grazed pasture rich in bracken fern and had suffered from severe intermittent haematuria from 3 to 4 years of age. on necropsy examination there were multiple haemorrhagic foci scattered over the mucosal surface of the urinary bladder. microscopically there were nests, cords and sheets of neoplastic cells infiltrating the lamina propria and muscularis propria. these had a syncytial appearance ...200818675990
study on the bacterial midgut microbiota associated to different brazilian populations of lutzomyia longipalpis (lutz & neiva) (diptera: psychodidae).the bacterial community associated with the midgut of three brazilian lutzomyia longipalpis (lutz & neiva) populations, two from endemic areas for visceral leishmaniasis (jacobina, bahia state and são luís, maranhão state) and one from a non-endemic area (lapinha cave, minas gerais state), was identified. five groups, 35 females each, from each population were separated; a total of 175 females per collecting area were analyzed. the species identification was based on molecular and traditional ba ...200819061048
management of human and animal bite wound infection: an overview.animal and human bite wounds can lead to serious infections. the organisms recovered generally originate from the biter's oral cavity and the victim's skin flora. anaerobes were isolated from more than two thirds of human and animal bite infections. streptococcus pyogenes is often recovered in human bites, pasteurella multocida in animal bites, eikenella corrodens in animal and human, capnocytophaga spp, neisseria weaveri, weeksella zoohelcum, neisseria canis, staphylococcus intermedius, nonoxid ...200919698283
complete genome sequence of weeksella virosa type strain (9751).weeksella virosa holmes et al. 1987 is the sole member and type species of the genus weeksella which belongs to the family flavobacteriaceae of the phylum bacteroidetes. twenty-nine isolates, collected from clinical specimens provided the basis for the taxon description. while the species seems to be a saprophyte of the mucous membranes of healthy man and warm-blooded animals a causal relationship with disease has been reported in a few instances. except for the ability to produce indole and to ...201121475590
[weeksella virosa]. 201122051619
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