Publications

wb e34 monoclonal antibody: further characterization and diagnostic use in bancroftian filariasis.	the wb e34 monoclonal antibody raised against wuchereria bancrofti microfilarial excretory secretory (mf es) antigen was reported to be useful in detecting the filarial antigen in w. bancrofti and brugia malayi infected sera. further studies in this laboratory showed that this monoclonal antibody reacts with a stage-specific antigen of w. bancrofti filarial parasite. wb e34 identified three antigenic components with molecular weights, 55, 57.5, and 63 kilodaltons (kd) in western blot analysis. t ...	1989	2681621
regulation of the f plasmid tray promoter in escherichia coli by host and plasmid factors.	f plasmid dna transfer (tra) gene expression in escherichia coli is regulated by chromosome- and f-encoded gene products. to study the relationship among these regulatory factors, we constructed low-copy plasmids containing a phi(tray'-'lacz)hyb gene that couples beta-galactosidase and lac permease synthesis to the f plasmid tray promoter. wild-type transformants maintained high levels of beta-galactosidase over a broad range of culture densities. primer extension analysis of tra mrna from f'lac ...	1991	2002497
microorganism selection and performance in bioslurry reactors treating pah-contaminated soil.	a continuous-flow reactor (cstr) and a soil slurry-sequencing batch reactor (ss-sbr) were operated in 81 vessels for 200 days to treat a soil contaminated with polycyclic aromatic hydrocarbons (pah). filtered slurry samples were used to quantify bulk biosurfactant concentrations and pah emulsification. concentrations of corynebacterium aquaticum, flavobacterium mizutaii, mycobacterium gastri, pseudomonas aeruginosa, and pseudomonas putida were determined using fatty acid methyl ester (fame) anal ...	2002	12361376
structural analysis of sphingophospholipids derived from sphingobacterium spiritivorum, the type species of genus sphingobacterium.	the unique feature of the genus sphingobacterium is the presence of sphingophospholipids and ceramides, besides diacylglycerophospholipids. as major cellular lipid components, five kinds of sphingophospholipids were purified from sphingobacterium spiritivorum atcc 33861(t), the type species of genus sphingobacterium. they were identified as ceramide phosphorylethanolamines (cerpe-1 and cerpe-2), ceramide phosphoryl-myo-inositols (cerpi-1 and cerpi-2), and ceramide phosphorylmannose (cerpm-1). th ...	2003	14729071
bioconversion of isopropanol by a solvent tolerant sphingobacterium mizutae strain.	the bioconversion of high concentration isopropanol (2-propanol, ipa) was investigated by a solvent tolerant strain of bacteria, which was identified as sphingobacterium mizutae st2 by partial 16s rdna gene sequencing. this strain of bacteria exhibited the ability to utilise high concentration isopropanol as the sole carbon source, with mineralization occurring via an acetone intermediate into central metabolism. the biodegradative performance of this strain for ipa was examined over a 2-38 g l( ...	2006	16758171
sphingobacterium daejeonense sp. nov., isolated from a compost sample.	a gram-negative, strictly aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated tr6-04(t), was isolated from compost and characterized taxonomically by using a polyphasic approach. the organism grew optimally at 30 degrees celsius and at ph 6.5-7.0. the isolate was positive for catalase and oxidase tests but negative for gelatinase, indole and h(2)s production. comparative 16s rrna gene sequence analysis showed that strain tr6-04(t) fell within the radiation of the clus ...	2006	16957095
hrt and nutrients affect bacterial communities grown on recirculation aquaculture system effluents.	in a recirculation aquaculture system the drumfilter effluent can be used as substrate for heterotrophic bacterial production, which can be recycled as feed. because the bacteria might contain pathogens, which could reduce its suitability as feed, it is important to characterize these communities. bacteria were produced in growth reactors under different conditions: 7 h hydraulic retention time (hrt) vs. 2 h, sodium acetate vs. molasses, and ammonia vs. nitrate. the community of the drumfilter e ...	2007	17371322
sphingobacterium composti sp. nov., isolated from cotton-waste composts.	a gram-negative, strictly aerobic, short rod-shaped, non-motile bacterial strain designated 4m24(t) was isolated from cotton-waste compost. analysis of the 16s rrna gene sequence of strain 4m24(t) revealed that it is a member of the genus sphingobacterium, sharing 88.5-94.5 % sequence similarity with type strains of the genus sphingobacterium and being most closely related to sphingobacterium daejeonense tr6-04(t) (94.5 % sequence similarity) and sphingobacterium mizutaii atcc 33299(t) (92.2 % s ...	2007	17625199
sphingobacterium anhuiense sp. nov., isolated from forest soil.	a gram-negative bacterium, designated strain cw 186(t), was isolated from forest soil in anhui province, china. cells of strain cw 186(t) were strictly aerobic, non-motile and rod-shaped. the strain grew optimally at 25-30 degrees c and ph 6.0-8.0. the major cellular fatty acids of strain cw 186(t) were iso-c(15:0) (32.2%), iso-c(17:0) 3-oh (9.8%) and summed feature 3 (iso-c(15:0) 2-oh and/or c(16:1)omega7c; 33.7%). the predominant isoprenoid quinone was mk-7. the g+c content of the genomic dna ...	2008	18768611
the utility of visual evoked potentials using hemifield stimulation and several check sizes in the evaluation of suspected multiple sclerosis.	one hundred and eleven patients with suspected multiple sclerosis (64 possible, 47 probable) and 16 with a definite diagnosis of ms were evaluated with pattern-reversal visual evoked potentials (veps), employing monocular full-field checks subtending 7', 14' and 28' of visual angle, and right and left hemifield 28' checks. thirty-seven patients (29%) had a completely normal study. sixty-six patients (52%) had abnormal responses to full-field 28' checks, and in 13 (10%) of these, additional abnor ...	2008	2446840
medical civil disobedience.		2008	2292675
early mr demonstration of spinal metastases in patients with normal radiographs and ct and radionuclide bone scans.	forty patients with known primary tumor and progressive back pain, suspected of having spinal metastatic disease, underwent magnetic resonance (mr) examinations of the thoracic and lumbosacral spine. conventional radiographs and ct scans of the spine were all normal. the radionuclide bone scans were equivocal. in 21 patients focal or diffuse vertebral mr abnormalities were detected. in nine patients the lesions were hypointense on t1 sequence, and the same lesions were demonstrated poorly or not ...	2009	2745777
sphingobacterium shayense sp. nov., isolated from forest soil.	a gram-staining-negative, yellow-coloured, strictly aerobic, non-spore-forming, rod-shaped bacterium, designated hs39(t), isolated from a soil sample collected from a natural populus euphratica forest in xinjiang, china, was characterized using a polyphasic approach. the isolate grew optimally at 30-37&emsp14;°c, at ph&emsp14;6.5-8.0 and with 0-3 % nacl. analysis of the 16s rrna gene sequence of strain hs39(t) revealed that it is a member of the genus sphingobacterium. sphingobacterium mizutaii ...	2010	19933581
[identification of pyrene degrading strains and the degrading characteristics research].	three bacterial strains j1, j2, j3 which could use pyrene as the sole carbon and energy sources were isolated from soils contaminated with polycyclic aromatic hydrocarbons (pahs) by enrichment culture. the strains were identified as pseudomonas aeruginosa, flavobacterium mizutaii, brevibacillus parabrevis according to the results of morphology, physiology and the phylogenetical analyses of 16s rdna sequence. it was observed that the three strains could use pyrene at the concentrations of 50, 100 ...	2010	20329546
sphingobacterium wenxiniae sp. nov., a cypermethrin-degrading species from activated sludge.	strain lqy-18t, a gram-negative, non-motile, non-spore-forming, non-flagellated, rod-shaped bacterium capable of degrading cypermethrin, was isolated from activated sludge of a waste-water treatment plant in china, and subjected to a polyphasic taxonomic investigation approach. growth occurred at 8-40 °c (optimum, 30 °c), at ph 5.0-10.0 (optimum, ph 7.0) and with 0-5 % nacl (w/v; optimum, 1 %). the predominant menaquinone was mk-7 (97 %) and the major fatty acids were summed feature 3 (comprisin ...	2011	21551333
sphingobacterium kyonggiense sp. nov., isolated from perchloroethylene (pce) contaminated soil and emended descriptions of sphingobacterium daejeonense and sphingobacterium mizutaii.	a gram-staining-negative, strictly aerobic, rod-shaped, non-motile strain designated 2-1-2t, was isolated from pce/tce contaminated soil in suwon, south korea. a polyphasic approach was applied to study the taxonomic position of strain 2-1-2t. strain 2-1-2t showed highest 16s rrna gene sequence similarity with sphingobacterium daejeonense tr6-04t (97.9 %) and sphingobacterium mizutaii atcc 33299t (97.1 %); sequence similarity to other sphingobacterium species was less than 93.0 %. phylogenetic a ...	2011	22180612
isolates belonging to cdc group ii-i predominantly belong to sphingobacterium mizutaii (yabuuchi et al., 1983), emended description of s. mizutaii and emended description of the genus sphingobacterium.	two clinical strains, nf 296 and nf 931, present in our collection, were identified biochemically as cdc group ii-i. determination of the 16s rrna gene sequence revealed highest similarity with sphingobacterium mizutaii. because these strains produced indole, whereas s. mizutaii has been described as indole negative, we investigated also the type strain and a reference strain of s. mizutaii, i.e. lmg 8340t (ccug 15907t) and lmg 8341 (ccug 15908), and found both strains to be indole production po ...	2011	22199213
sphingobacterium hotanense sp. nov., isolated from soil of a populus euphratica forest, and emended descriptions of sphingobacterium daejeonense and sphingobacterium shayense.	a novel gram-staining-negative bacterial strain, designated xh4(t), was isolated from soil of a populus euphratica forest in the hotan river valley, xinjiang uyghur autonomous region, pr china. the cells were strictly aerobic, non-motile, short rods. the isolate grew optimally at 37 °c and at ph 7.0-8.0. based on 16s rrna gene sequence analysis, strain xh4(t) belonged to the genus sphingobacterium and was closely related to sphingobacterium mizutaii atcc 33299(t) (96.1 % sequence similarity). th ...	2013	22611196
[histochemical research on mandibular adamantinoma in a young patient. remarks on the "alcian" sensitive stromatic substance].		2015	4515247
[presence of antibodies to fowl proteins in the workers on an industrial fattening farm].		2016	4997791
sphingobacterium cellulitidis sp. nov., isolated from clinical and environmental sources.	we have determined the taxonomic position of two isolates of the genus sphingobacterium. the first isolate, r-53603t, was obtained from purulent discharge from the toe of a cellulitis patient in kuwait. comparative 16s rrna gene sequence analysis revealed 99.87% identity of r-53603t with environmental isolate p031 (= r-53745) originating from activated sludge in singapore. both isolates were phylogenetically positioned on the same sub-branch. highest 16s rrna gene sequence similarity was found w ...	2017	28141505
sphingobacteriumsoli sp. nov., isolated from soil.	a gram-stain-negative, strictly aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated yim x0211t, was isolated from a soil sample of shiling county, yunnan province, south-west china. the new isolate was characterized taxonomically by using a polyphasic approach. the strain grew optimally at 30 °c, at ph 7.0 and with 0-3 % (w/v) nacl. it was positive for catalase and oxidase but negative for h2s production. comparative 16s rrna gene sequence analysis showed that strain ...	2017	28699577