Publications
Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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sulfate reduction by a desulfovibrio species isolated from sheep rumen. | several dissimilatory, sulfate-reducing bacteria were isolated from the rumen fluid of sheep fed purified diets containing sulfate. one isolate, strain d, was selected for characterization. this organism is a nonsporeforming, obligately anaerobic, mesophilic, nonmotile, gram-negative, straight rod. cell-free extracts show absorption maxima for cytochrome c(3) and desulfoviridin, characteristic of desulfovibrio. carbohydrates, as a sole carbon source, will support growth. lactate supports growth ... | 1974 | 4472525 |
bouyant density, conversion formulae, and the mole percent guanosine + cytosine content of desulfovibrio sp. | a reevaluation of the original buoyant density conversion formula used to calculate the molar percentage guanosine + cytosine (% g + c) contents of the accepted species of genus desulfovibrio has been undertaken. it would appear that the formula used gives values 4-5% lower than those obtained using formulae more generally cited in modern literature. recalculations of % g + c content values for lesulfovibrio dna are presented using the formulae of three different workers, and are compared with t ... | 1976 | 974922 |
dithionite reduction in the presence of a tetrapyrrole-containing fraction from the desulfoviridin of desulfovibrio gigas. | low-molecular-weight fractions obtained from the desulfoviridin of d. gigas and from the growth medium of desulfovibrio sp. 10455 promoted the reduction of sodium dithionite to sulphide in the presence of reduced methylviologen. these fractions contained a tetrapyrrole of the isobacteriochlorin type which was not complexed with iron, nor was it complexed with protein. the observations are discussed in relation to the function of sulphite reductases in the sulphate-reducing bacteria. | 1977 | 901305 |
dimethyl sulfoxide as an electron acceptor for anaerobic growth. | the isolation from lake mud of a bacterium which can use dimethyl sulfoxide (dmso) as an electron acceptor for growth is described. the isolate, called strain dl-1, was a small, gram negative, non-motile spiral. the sole product of dmso reduction was dimethyl sulfide (dms). other electron acceptors used by the isolate included sulfite, thiosulfate, elemental sulfur, methionine sulfoxide, tetramethylene sulfoxide, nitrate, and oxygen (microaerophilically). sulfate was not reduced and could not ev ... | 1978 | 414686 |
enumeration and relative importance of acetylene-reducing (nitrogen-fixing) bacteria in a delaware salt marsh. | three groups of n(2)-fixing bacteria were enumerated from the top 1 cm of the surface in four vegetational areas in a delaware salt marsh. the results over the 9-month sampling period showed that there were no discernible seasonal patterns for any of the groups enumerated (azotobacter sp., clostridium sp., and desulfovibrio sp.). azotobacter sp. was present in numbers of 10 per g of dry mud, whereas the two anaerobic fixers were present in much lower numbers (10 to 10 per g of dry mud). there we ... | 1980 | 16345564 |
propionate-degrading bacterium, syntrophobacter wolinii sp. nov. gen. nov., from methanogenic ecosystems. | a new genus and species of a nonmotile gram-negative rod, syntrophobacter wolinii, is the first bacterium described which degrades propionate only in coculture with an h(2)-using organism and in the absence of light or exogenous electron acceptors such as o(2), sulfate, or nitrate. it was isolated from methanogenic enrichments from an anaerobic municipal sewage digestor, using anaerobic roll tubes containing a medium with propionate as the energy source in association with an h(2)-using, sulfate ... | 1980 | 16345640 |
syntrophomonas wolfei gen. nov. sp. nov., an anaerobic, syntrophic, fatty acid-oxidizing bacterium. | an anaerobic, nonphototrophic bacterium that beta-oxidizes saturated fatty acids (butyrate through octanoate) to acetate or acetate and propionate using protons as the electron acceptor (h(2) as electron sink product) was isolated in coculture with either a non-fatty acid-degrading, h(2)-utilizing desulfovibrio sp. or methanogens. three strains of the bacterium were characterized and are described as a new genus and species, syntrophomonas wolfei. s. wolfei is a gram-negative, slightly helical r ... | 1981 | 16345745 |
conversion of cellulose to methane and carbon dioxide by triculture of acetivibrio cellulolyticus, desulfovibrio sp., and methanosarcina barkeri. | the fermentation of cellulose by monocultures of acetivibrio cellulolyticus and cocultures of a. cellulolyticus-methanosarcina barkeri, a. cellulolyticus-desulfovibrio sp., and a. cellulolyticus-m. barkeri-desulfovibrio sp. was studied. the monoculture produced ethanol, acetate, h(2), and co(2). more acetate and less ethanol was formed by the cocultures than by the monoculture. acetate was utilized by m. barkeri in coculture with a. cellulolyticus after a lag period, whereas ethanol was metaboli ... | 1981 | 16345841 |
microbial transformations of inorganic sulphur compounds in soil under conditions of heterocontinuous cultivation. | development and activity of the association of the sulphur cycle bacteria, represented by thiobacillus thioparus and desulfovibrio sp., were followed in chernozem soil continuously supplemented with sodium thiosulphate. the technique of heterocontinuous cultivation made it possible (i) to determine changes in the individual components of microflora involved in successive metabolic steps, their time and space sequence, (ii) to follow changes in the transformations of substrate and formation of me ... | 1982 | 7173746 |
methanogenesis from choline by a coculture of desulfovibrio sp. and methanosarcina barkeri. | a sulfate-reducing vibrio was isolated from a methanogenic enrichment with choline as the sole added organic substrate. this organism was identified as a member of the genus desulfovibrio and was designated desulfovibrio strain g1. in a defined medium devoid of sulfate, a pure culture of desulfovibrio strain g1 fermented choline to trimethylamine, acetate, and ethanol. in the presence of sulfate, more acetate and less ethanol were formed from choline than in the absence of sulfate. when grown in ... | 1983 | 16346162 |
growth of a strictly anaerobic bacterium on furfural (2-furaldehyde). | a strictly anaerobic bacterium was isolated from a continuous fermentor culture which converted the organic constituents of sulfite evaporator condensate to methane and carbon dioxide. furfural is one of the major components of this condensate. this furfural isolate could degrade furfural as the sole source of carbon and energy in a defined mineral-vitamin-sulfate medium. acetic acid was the major fermentation product. this organism could also use ethanol, lactate, pyruvate, or fumarate and cont ... | 1983 | 16346423 |
nonlinear estimation of monod growth kinetic parameters from a single substrate depletion curve. | monod growth kinetic parameters were estimated by fitting sigmoidal substrate depletion data to the integrated monod equation, using nonlinear least-squares analysis. when the initial substrate concentration was in the mixed-order region, nonlinear estimation of simulated data sets containing known measurement errors provided accurate estimates of the mu max, ks, and y values used to create these data. nonlinear regression analysis of sigmoidal substrate depletion data was also evaluated for h2- ... | 1983 | 6870238 |
isolation and partial characterization of bacteria in an anaerobic consortium that mineralizes 3-chlorobenzoic acid. | a methanogenic consortium able to use 3-chlorobenzoic acid as its sole energy and carbon source was enriched from anaerobic sewage sludge. seven bacteria were isolated from the consortium in mono- or coculture. they included: one dechlorinating bacterium (strain dcb-1), one benzoate-oxidizing bacterium (strain bz-2), two butyrate-oxidizing bacteria (strains sf-1 and nsf-2), two h(2)-consuming methanogens (methanospirillum hungatei pm-1 and methanobacterium sp. strain pm-2), and a sulfate-reducin ... | 1984 | 16346648 |
hydrogen-using bacteria in a methanogenic acetate enrichment culture. | in a study of the anaerobic utilization of acetate, an enrichment culture of sewage sludge organisms was initiated with calcium acetate as the sole carbon and energy source. a mixed bacterial population became established from which 14 anaerobic species were isolated. two of the isolates were methanogenic bacteria but only one of these, methanosarcina barkeri, utilised acetate as an energy source in axenic culture. the other methanogenic isolate, a methanobacterium sp., utilised h2/co2 but not a ... | 1984 | 6423605 |
effects of hydrogen pressure during growth and effects of pregrowth with hydrogen on acetate degradation by methanosarcina species. | methanosarcina barkeri 227 and methanosarcina mazei s-6 grew with acetate as the substrate; we found little effect of h(2) on the rate of aceticlastic growth in the presence of various h(2) pressures between 2 and 810 pa. we used physical (h(2) addition or flushing the headspace to remove h(2)) and biological (h(2)-producing or -utilizing bacteria in cocultures) methods for controlling h(2) pressure in methanosarcina cultures growing on acetate. added h(2) (ca. 100 pa) was removed rapidly (a few ... | 1987 | 16347269 |
properties of desulfovibrio carbinolicus sp. nov. and other sulfate-reducing bacteria isolated from an anaerobic-purification plant. | several sulfate-reducing microorganisms were isolated from an anaerobic-purification plant. four strains were classified as desulfovibrio desulfuricans, desulfovibrio sapovorans, desulfobulbus propionicus, and desulfovibrio sp. the d. sapovorans strain contained poly-beta-hydroxybutyrate granules and seemed to form extracellular vesicles. a fifth isolate, desulfovibrio sp. strain edk82, was a gram-negative, non-spore-forming, nonmotile, curved organism. it was able to oxidize several substrates, ... | 1987 | 16347324 |
bioenergetic conditions of butyrate metabolism by a syntrophic, anaerobic bacterium in coculture with hydrogen-oxidizing methanogenic and sulfidogenic bacteria. | the butyrate-oxidizing, proton-reducing, obligately anaerobic bacterium nsf-2 was grown in batch cocultures with either the hydrogen-oxidizing bacterium methanospirillum hungatei pm-1 or desulfovibrio sp. strain ps-1. metabolism of butyrate occurred in two phases. the first phase exhibited exponential growth kinetics (phase a) and had a doubling time of 10 h. this value was independent of whether nsf-2 was cultured with a methanogen or a sulfate reducer and likely represents the maximum specific ... | 1988 | 16347645 |
phospholipid fatty acid composition of the syntrophic anaerobic bacterium syntrophomonas wolfei. | the membrane phospholipid fatty acids (plfas) from several cocultures and a pure culture of syntrophomonas wolfei were determined by capillary column gas chromatography. cocultures of s. wolfei with a desulfovibrio sp. contained plfas from both organisms, whereas plfas from a coculture with methanospirillum hungatei contained very little biomass to analyze. the pure culture of s. wolfei grown on crotonate provided the best material for analysis of the plfas. the predominant plfas of s. wolfei we ... | 1988 | 16347667 |
hydrogen production and deuterium-proton exchange reactions catalyzed by desulfovibrio nickel(ii)-substituted rubredoxins. | the nickel tetrahedral sulfur-coordinated core formed upon metal replacement of the native iron in desulfovibrio sp. rubredoxins is shown to mimic the reactivity pattern of nickel-containing hydrogenases with respect to hydrogen production, deuterium-proton exchange, and inhibition by carbon monoxide. | 1988 | 16594005 |
methanogenesis from ethanol by defined mixed continuous cultures. | methanogenesis from ethanol by defined mixed continuous cultures was studied. under sulfate-free conditions, a desulfovibrio strain was used as the ethanol-degrading species producing acetic acid and hydrogen. in a two-membered mutualistic coculture, the hydrogen was converted to methane by a methanobacterium sp. and ph was maintained at neutrality by the addition of alkali. introduction of a third species, the acetate-utilizing methanosarcina mazei, obviated the need for external ph control. me ... | 1989 | 16347852 |
bioaccumulation and chemical modification of tc by soil bacteria. | bioaccumulation and chemical modification of pertechnetate (tco4-) by aerobically and anaerobically grown soil bacteria and by pure cultures of sulfate-reducing bacteria (desulfovibrio sp.) were studied to gain insight on the possible mechanisms by which bacteria can affect the solubility of tc in soil. aerobically grown bacteria had no apparent effect on tco4-; they did not accumulate tc nor modify its chemical form. anaerobically grown bacteria exhibited high bioaccumulation and reduced tco4-, ... | 1989 | 2547734 |
oxidation of benzaldehydes to benzoic acid derivatives by three desulfovibrio strains. | desulfovibrio vulgaris marburg, "desulfovibrio simplex" xvi, and desulfovibrio sp. strain mp47 used benzaldehydes such as vanillin, 3,4,5-trimethoxybenzaldehyde, protocatechualdehyde, syringaldehyde, p-anisaldehyde, p-hydroxybenzaldehyde, and 2-methoxybenzaldehyde as electron donors for sulfate reduction and carbon dioxide and/or components of yeast extract as carbon sources for cell synthesis. the aldehydes were oxidized to their corresponding benzoic acids. the three sulfate reducers oxidized ... | 1990 | 2389937 |
anaerobic degradation of 1,2-propanediol by a new desulfovibrio strain and d. alcoholovorans. | a sulfate-reducing bacterium, strain hdv, was isolated from the anoxic soil of a ricefield using lactate as electron donor. cells were gram-negative, motile, nonsporulating curved rods, with single polar flagella. substrates were incompletely oxidized to acetate and included glycerol, 1,2- and 1,3-propanediol. sulfate, sulfite, thiosulfate, elemental sulfur, fumarate, maleate, and malate were utilized as electron acceptors. pyruvate, fumarate, maleate, malate and dihydroxyacetone were fermented. ... | 1992 | 1332638 |
trinitrotoluene (tnt) as a sole nitrogen source for a sulfate-reducing bacterium desulfovibrio sp. (b strain) isolated from an anaerobic digester. | a sulfate-reducing bacterium (srb), desulfovibrio sp. (b strain), isolated from a continuous anaerobic digester (boopathy and daniels, current microbiology, 23:327-332, 1991) was found to use 2,4,6-trinitrotoluene (tnt) as sole nitrogen source. this bacterium also used nitrate, nitrite, and ammonium as nitrogen source. a long lag period was noticed when tnt or nitrite was used as nitrogen source. nitrate, nitrite and tnt also served as electron acceptor in the absence of sulfate for this bacteri ... | 1992 | 1368976 |
nitroaromatic compounds serve as nitrogen source for desulfovibrio sp. (b strain). | a sulfate-reducing bacterium, desulfovibrio sp. (b strain), isolated from a continuous anaerobic digester, used various nitroaromatic compounds such as 2,4-dinitrophenol, 2,4-dinitrotoluene, and 2,6-dinitrotoluene as sole nitrogen sources for growth and also used these compounds as electron acceptors in the absence of sulfate in the culture medium. more than 60% of the nitroaromatics were transformed within 6 days of incubation. the organism also used aniline as sole nitrogen source, but not as ... | 1993 | 8500012 |
assignment of fatty acid-beta-oxidizing syntrophic bacteria to syntrophomonadaceae fam. nov. on the basis of 16s rrna sequence analyses. | after enrichment from chinese rural anaerobic digestor sludge, anaerobic, sporing and nonsporing, saturated fatty acid-beta-oxidizing syntrophic bacteria were isolated as cocultures with h2- and formate-utilizing methanospirillum hungatei or desulfovibrio sp. strain g-11. the syntrophs degraded c4 to c8 saturated fatty acids, including isobutyrate and 2-methylbutyrate. they were adapted to grow on crotonate and were isolated as pure cultures. the crotonate-grown pure cultures alone did not gr ... | 1993 | 11536533 |
phylogenetic analysis of syntrophobacter wolinii reveals a relationship with sulfate-reducing bacteria. | a 16s rrna sequence analysis of syntrophobacter wolinii was done by using pcr amplification of the 16s rrna-genes from dna isolated from the s. wolinii-desulfovibrio sp. coculture. phylogenetic analysis using the obtained sequence revealed that s. wolinii was not related to bacteria growing syntrophically on other fatty acids than propionate, but was related to sulfate-reducing bacteria. the closest related bacteria are desulfomonile tiedjei and desulfoarculus baarsii. | 1993 | 7692834 |
intracellular campylobacter-like organism from ferrets and hamsters with proliferative bowel disease is a desulfovibrio sp. | proliferative bowel disease is an intestinal disorder of a variety of domestic animals associated with the presence of an intracellular campylobacter-like organism (iclo). we have identified the iclo obtained from a ferret with proliferative colitis by 16s rrna sequence analysis. in this ferret, proliferative bowel tissue containing the iclo had translocated to the mesenteric lymph nodes, omentum, and liver. the 16s rrna genes of the iclo were amplified from an infected fragment of extraintestin ... | 1994 | 8051249 |
acetogenesis from dichloromethane by a two-component mixed culture comprising a novel bacterium. | a strictly anaerobic two-component culture able to grow exponentially with a doubling time of 20 h on a medium containing dichloromethane as the carbon and energy source was characterized. on a medium without sulfate, we observed (per mol of dichloromethane) a mass balance of 2 mol of chloride, 0.26 mol of acetate, 0.05 mol of formate, and 0.25 mol of carbon in biomass. one component of the culture, strain dmb, was identified by a 16s ribosomal dna analysis as a desulfovibrio sp. the other compo ... | 1995 | 16535097 |
[isolation and reassociation of acetogen and methanogen in a syntrophobic coculture degrading butyrate anaerobically]. | anaerobic coculture bf2 which degraded butyrate into acetate and produced methane was isolated from granular methanogenic sludge. the coculture is associated syntrophically the syntrophomonas subsp. saponavida strain cf2 with methanobacterium formicicum strain mf2 and appeared to degraded c4 approximately c18 fatty acids including isobutyrate. the optimal temperature and ph for growth was 37 degrees c and 7.7 respectively. the strain cf2 was obtained in pure culture with crotonate as substrate a ... | 1995 | 8745550 |
degradative capacities and 16s rrna-targeted whole-cell hybridization of sulfate-reducing bacteria in an anaerobic enrichment culture utilizing alkylbenzenes from crude oil. | a mesophilic sulfate-reducing enrichment culture growing anaerobically on crude oil was used as a model system to study which nutritional types of sulfate-reducing bacteria may develop on original petroleum constituents in oil wells, tanks, and pipelines. chemical analysis of oil hydrocarbons during growth revealed depletion of toluene and o-xylene within 1 month and of m-xylene, o-ethyltoluene, m-ethyltoluene, m-propyltoluene, and m-isopropyltoluene within approximately 2 months. in anaerobic c ... | 1996 | 8837415 |
activation and degradation of benzoate, 3-phenylpropionate and crotonate by syntrophus buswellii strain ga. evidence for electron-transport phosphorylation during crotonate respiration. | a strictly anaerobic, benzoate-degrading bacterium, syntrophus buswellii strain ga, was able to degrade benzoate or 3-phenylpropionate to acetate, co2 and h2, if the hydrogen partial pressure was sufficiently low. the hydrogen was removed in syntrophic coculture by methanospirillum hungatei or by desulfovibrio sp. through interspecies hydrogen transfer or in pure culture by the use of crotonate as reducible cosubstrate. alternatively, s. buswellii strain ga could grow in pure culture with croton ... | 1996 | 8867639 |
interspecies acetate transfer influences the extent of anaerobic benzoate degradation by syntrophic consortia. | benzoate degradation by an anaerobic, syntrophic bacterium, strain sb, in coculture with desulfovibrio sp. strain g-11 reached a threshold value which depended on the amount of acetate added and ranged from about 2.5 to 29.9 (mu)m. increasing acetate concentrations also uncompetitively inhibited benzoate degradation. the apparent v(infmax) and apparent k(infm) for benzoate degradation decreased with increasing acetate concentration, but the benzoate degradation capacities (v(infmax)/k(infm)) of ... | 1996 | 16535215 |
cometabolic transformation and cleavage of nitrodiphenylamines by three newly isolated sulfate-reducing bacterial strains. | three sulfate-reducing bacterial strains (desulfovibrio sp. strain shv, desulfococcus sp. strain whc, and desulfomicrobium sp. strain whb) with the capacity to cometabolize 2-nitrodiphenylamine, 4-nitrodiphenylamine, and 2,4-dinitrodiphenylamine were newly isolated. before breaking down the diphenylamine structure, these strains cometabolically reduce the nitrodiphenylamines to the corresponding aminodiphenylamines during anaerobic oxidation of the growth substrate lactate (desulfovibrio strain ... | 1996 | 16535317 |
biological sulfate reduction using synthesis gas as energy and carbon source. | biological sulfate reduction was studied in laboratory-scale gas-lift reactors. synthesis gas (gas mixtures of h(2)/co/co(2)) was used as energy and carbon source. the required biomass retention was obtained by aggregation and immobilization on pumice particles. special attention was paid to the effect of co addition on the sulfate conversion rate, aggregation, and aggregate composition.addition of 5% co negatively affected the overall sulfate conversion rate; i.e., it dropped from 12-14 to 6-8 ... | 1996 | 18626930 |
taurine reduction in anaerobic respiration of bilophila wadsworthia rzatau. | organosulfonates are important natural and man-made compounds, but until recently (t. j. lie, t. pitta, e. r. leadbetter, w. godchaux iii, and j. r. leadbetter. arch. microbiol. 166:204-210, 1996), they were not believed to be dissimilated under anoxic conditions. we also chose to test whether alkane- and arenesulfonates could serve as electron sinks in respiratory metabolism. we generated 60 anoxic enrichment cultures in mineral salts medium which included several potential electron donors and ... | 1997 | 9143131 |
unusual ligand structure in ni-fe active center and an additional mg site in hydrogenase revealed by high resolution x-ray structure analysis. | the hydrogenase of desulfovibrio sp. catalyzes the reversible oxidoreduction of molecular hydrogen, in conjunction with a specific electron acceptor, cytochrome c3. the ni-fe active center of desulfovibrio hydrogenase has an unusual ligand structure with non-protein ligands. an atomic model at high resolution is required to make concrete assignment of the ligands which coordinate the ni-fe center. these in turn will provide insight into the mechanism of electron transfer, during the reaction cat ... | 1997 | 9438867 |
isolation from estuarine sediments of a desulfovibrio strain which can grow on lactate coupled to the reductive dehalogenation of 2,4, 6-tribromophenol. | strain tbp-1, an anaerobic bacterium capable of reductively dehalogenating 2,4,6-tribromophenol to phenol, was isolated from estuarine sediments of the arthur kill in the new york/new jersey harbor. it is a gram-negative, motile, vibrio-shaped, obligate anaerobe which grows on lactate, pyruvate, hydrogen, and fumarate when provided sulfate as an electron acceptor. the organism accumulates acetate when grown on lactate and sulfate, contains desulfoviridin, and will not grow in the absence of nacl ... | 1999 | 10049873 |
third human isolate of a desulfovibrio sp. identical to the provisionally named desulfovibrio fairfieldensis. | desulfovibrio fairfieldensis was isolated from the urine sample of a patient with a urinary tract infection and meningoencephalitis. it was identified by 16s rrna gene amplification and sequencing. | 1999 | 10449514 |
adhesion of biodegradative anaerobic bacteria to solid surfaces. | in order to exploit the ability of anaerobic bacteria to degrade certain contaminants for bioremediation of polluted subsurface environments, we need to understand the mechanisms by which such bacteria partition between aqueous and solid phases, as well as the environmental conditions that influence partitioning. we studied four strictly anaerobic bacteria, desulfomonile tiedjei, syntrophomonas wolfei, syntrophobacter wolinii, and desulfovibrio sp. strain g11, which theoretically together can co ... | 1999 | 10543826 |
metabolism of benzoate, cyclohex-1-ene carboxylate, and cyclohexane carboxylate by "syntrophus aciditrophicus" strain sb in syntrophic association with h(2)-using microorganisms. | the metabolism of benzoate, cyclohex-1-ene carboxylate, and cyclohexane carboxylate by "syntrophus aciditrophicus" in cocultures with hydrogen-using microorganisms was studied. cyclohexane carboxylate, cyclohex-1-ene carboxylate, pimelate, and glutarate (or their coenzyme a [coa] derivatives) transiently accumulated during growth with benzoate. identification was based on comparison of retention times and mass spectra of trimethylsilyl derivatives to the retention times and mass spectra of authe ... | 2001 | 11282627 |
colonic infection by bilophila wadsworthia in pigs. | bilophila wadsworthia is a common inhabitant of the human colon and has been associated with appendicitis and other local sites of inflammation in humans. challenge-exposure or prevalence studies in laboratory and other animals have not been reported. b. wadsworthia is closely related phylogenetically to desulfovibrio sp. and lawsonia intracellularis, which are considered colon pathogens. we developed a pcr specific for b. wadsworthia dna. samples of bacterial dna extracted from the feces of pig ... | 2001 | 11283090 |
coexistence of a sulphate-reducing desulfovibrio species and the dehalorespiring desulfitobacterium frappieri tce1 in defined chemostat cultures grown with various combinations of sulfate and tetrachloroethene. | a two-member co-culture consisting of the dehalorespiring desulfitobacterium frappieri tce1 and the sulphate-reducing desulfovibrio sp. strain sulf1 was obtained via anaerobic enrichment from soil contaminated with tetrachloroethene (pce). in this co-culture, pce dechlorination to cis-dichloroethene was due to the activity of the dehalorespiring bacterium only. chemostat experiments with lactate as the primary electron donor for both strains along with varying sulphate and pce concentrations sho ... | 2001 | 11321548 |
the influence of fluid shear and aici3 on the material properties of pseudomonas aeruginosa pao1 and desulfovibrio sp. ex265 biofilms. | an understanding of the material properties of biofilms is important when describing how biofilms physically interact with their environment. in this study, aerobic biofilms of pseudomonas aeruginosa pao1 and anaerobic sulfate-reducing bacteria (srb) biofilms of desulfovibrio sp. ex265 were grown under different fluid shear stresses (tau g) in a chemostat recycle loop. individual biofilm microcolonies were deformed by varying the fluid wall shear stress (tau w). the deformation was quantified in ... | 2001 | 11381956 |
mechanistic study of microbial control of hydrogen sulfide production in oil reservoirs. | microbial control of biogenic production of hydrogen sulfide in oil fields was studied in a model system consisting of pure cultures of the nitrate-reducing, sulfide-oxidizing bacterium (nr-sob) thiomicrospira sp. strain cvo and the sulfate-reducing bacterium (srb) desulfovibrio sp. strain lac6, as well as in microbial cultures enriched from produced water of a canadian oil reservoir. the presence of nitrate at concentrations up to 20 mm had little effect on the rate of sulfate reduction by a pu ... | 2001 | 11427944 |
conformational component in the coupled transfer of multiple electrons and protons in a monomeric tetraheme cytochrome. | cell metabolism relies on energy transduction usually performed by complex membrane-spanning proteins that couple different chemical processes, e.g. electron and proton transfer in proton-pumps. there is great interest in determining at the molecular level the structural details that control these energy transduction events, particularly those involving multiple electrons and protons, because tight control is required to avoid the production of dangerous reactive intermediates. tetraheme cytochr ... | 2001 | 11551953 |
control of biogenic h(2)s production with nitrite and molybdate. | the effects of the metabolic inhibitors, sodium nitrite and ammonium molybdate, on production of h(2)s by a pure culture of the sulfate-reducing bacterium (srb) desulfovibrio sp. strain lac6 and a consortium of srb, enriched from produced water of a canadian oil field, were investigated. addition of 0.1 mm nitrite or 0.024 mm molybdate at the start of growth prevented the production of h(2)s by strain lac6. with exponentially growing cultures, higher levels of inhibitors, 0.25 mm nitrite or 0.09 ... | 2001 | 11571618 |
impact of nitrate-mediated microbial control of souring in oil reservoirs on the extent of corrosion. | the effect of microbial control of souring on the extent of corrosion was studied in a model system consisting of pure cultures of the nitrate-reducing, sulfide-oxidizing bacterium (nr-sob) thiomicrospira sp. strain cvo and the sulfate-reducing bacterium (srb) desulfovibrio sp. strain lac6, as well as in an srb consortium enriched from produced water from a canadian oil reservoir. the average corrosion rate induced by the srb consortium (1.4 g x m(-2) x day(-1)) was faster than that observed in ... | 2001 | 11587574 |
comparative analysis of polychlorinated biphenyl-dechlorinating communities in enrichment cultures using three different molecular screening techniques. | the catalysts for many microbially mediated environmental processes such as the dechlorination of polychlorinated biphenyls (pcbs) have been difficult to identify by traditional isolation techniques. numerous, as yet unsuccessful, attempts have been made to isolate and culture the dechlorinating species. to overcome this limitation, amplified rdna restriction analysis (ardra) of a clone library, denaturing gradient gel electrophoresis (dgge) and terminal restriction fragment length polymorphism ... | 2001 | 11846761 |
desulfovibrio sp. genes involved in the respiration of sulfate during metabolism of hydrogen and lactate. | to develop a better understanding of respiration by sulfate-reducing bacteria, we examined transcriptional control of respiratory genes during growth with lactate or hydrogen as an electron donor. rna extracts of desulfovibrio desulfuricans subsp. aestuarii were analyzed by using random arbitrarily primed pcr. rna was reverse transcribed under low-stringency conditions with a set of random primers, and candidate cdnas were cloned, sequenced, and characterized by blast analysis. putative differen ... | 2002 | 11916715 |
thermodynamic and kinetic characterization of trihaem cytochrome c3 from desulfuromonas acetoxidans. | trihaem cytochrome c3 (also known as cytochrome c551.5 and cytochrome c7) is isolated from the periplasmic space of desulfuromonas acetoxidans, a sulfur-reducing bacterium. thermodynamic and kinetic data for the trihaem cytochrome c3 are presented and discussed in the context of the possible physiological implications of its functional properties with respect to the natural habitat of d. acetoxidans, namely as a symbiont with green sulfur bacteria working as a mini-sulfuretum. the thermodynamic ... | 2002 | 12423372 |
a mechano-chemical model for energy transduction in cytochrome c oxidase: the work of a maxwell's god. | cytochrome c3 has a central role in the energetics of desulfovibrio sp., where it performs an electroprotonic energy transduction step. this process uses a network of cooperativities, largely based on anti-coulomb components, resulting from a mechano-chemical energy coupling mechanism. this mechanism provides a model coherent with the data available for the redox chemistry of haem a of cytochrome c oxidase and its link to the activation of protons. a crucial feature of the model is an anti-coulo ... | 2002 | 12482576 |
the influence of fluid shear on the structure and material properties of sulphate-reducing bacterial biofilms. | biofilms of sulphate-reducing desulfovibrio sp. ex265 were grown in square section glass capillary flow cells under a range of fluid flow velocities from 0.01 to 0.4 m/s (wall shear stress, tau(w), from 0.027 to 1.0 n/m(2)). in situ image analysis and confocal scanning laser microscopy revealed biofilm characteristics similar to those reported for aerobic biofilms. biofilms in both flow cells were patchy and consisted of cell clusters separated by voids. length-to-width ratio measurements (l(c): ... | 2002 | 12483477 |
identification of a bacterium that specifically catalyzes the reductive dechlorination of polychlorinated biphenyls with doubly flanked chlorines. | a microorganism whose growth is linked to the dechlorination of polychlorinated biphenyls (pcbs) with doubly flanked chlorines was identified. identification was made by reductive analysis of community 16s ribosomal dna (rdna) sequences from a culture enriched in the presence of 2,3,4,5-tetrachlorobiphenyl (2,3,4,5-cb), which was dechlorinated at the para position. denaturing gradient gel electrophoresis (dgge) analysis of total 16s rdna extracted from the culture led to identification of three ... | 2002 | 11823222 |
biotechnological potential of sulfate-reducing bacteria for transformation of nitrocellulose. | the biotransformation of nc by desulfovibrio sp. was studied. the mass of nc was decreased by 4.9-9.3%. the rate of nc transformation was between 46 and 73 mg nc per mg of bacterial protein in 10 days. moreover, n content (%n) in the remaining nc was reduced by 2-12%. the inhibitory effect of nc was clearly expressed when the growth of d. desulfuricans 1388 in lactate/sulfate medium was initiated. the growth rate of bacteria was 1.5-fold greater when nc was not added (0.074 and 0.05 h(-1) respec ... | 2002 | 16887675 |
[analysis of the anaerobic microbial community capable of degrading p-toluene sulphonate]. | three strains of clostridium sp., 14 (vkm b-2201), 42 (vkm b-2202), and 21 (vkm b-2279), two methanogens, methanobacterium formicicum mh (vkm b-2198) and methanosarcina mazei mm (vkm b-2199), and one sulfate-reducing bacterium, desulfovibrio sp. sr1 (vkm b-2200), were isolated in pure cultures from an anaerobic microbial community capable of degrading p-toluene sulfonate. strain 14 was able to degrade p-toluene sulfonate in the presence of yeast extract and bactotryptone and, like strain 42, to ... | 2003 | 14768540 |
sulphate-reducing bacteria, palladium and the reductive dehalogenation of chlorinated aromatic compounds. | the surfaces of cells of desulfovibrio desulfuricans, desulfovibrio vulgaris and a new strain, desulfovibrio sp. 'oz-7' were used to manufacture a novel bioinorganic catalyst via the reduction of pd(ii) to pd(0) at the cell surface using hydrogen as the electron donor. the ability of the palladium coated (palladised) cells to reductively dehalogenate chlorophenol and polychlorinated biphenyl species was demonstrated. dried, palladised cells of d. desulfuricans, d. vulgaris and desulfovibrio sp. ... | 2003 | 12877464 |
adhesion of anaerobic microorganisms to solid surfaces and the effect of sequential attachment on adhesion characteristics. | the attachment of three anaerobic microorganisms, desulfomonile tiedjei, syntrophomonas wolfei, and desulfovibrio sp. strain g11, was investigated to determine if the presence of one species could influence the adhesion of another species to glass surfaces. the results indicated that the numbers and distribution of attached cells of one species could be influenced considerably by the presence of another species and the order in which the test species were exposed to the surface. d. tiedjei was f ... | 2003 | 14618685 |
a novel approach to investigate biofilm accumulation and bacterial transport in porous matrices. | knowledge of bacterial transport through, and biofilm growth in, porous media is vitally important in numerous natural and engineered environments. despite this, porous media systems are generally oversimplified and the local complexity of cell transport, biofilm formation and the effect of biofilm accumulation on flow patterns is lost. in this study, cells of the sulphate-reducing bacterium, desulfovibrio sp. ex265, accumulated primarily on the leading faces of obstructions and developed into b ... | 2004 | 14756882 |
[stages of biofilm formation by sulfate-reducing bacteria]. | taxis to fe3+ ions and adhesion to steel-3 of sulphate-reducing bacteria different by corrosion activity have been investigated. it has been shown that taxis activity of cells from the postgate medium "b" was higher than from the buffer. aggressive strains desulfovibrio indonensis, desulfovibrio sp. possessed higher activity taxis with respect to fe3+ ions. it has been noted that aggressive strains of sulphate-reducing bacteria adhered more actively to the steel surface and formed more powerful ... | 2004 | 15456221 |
[effect of corrosion inhibitor on adhesion of sulfate-reducing bacteria to steel and their production of exopolymer complex]. | it was shown in the laboratory investigations that the cells of sulphate-reducing bacteria of both aggressive desulfovibrio sp. strain kiev-10 and nonaggressive desulfovibrio desulfuricans strain kiev-45 strains can produce exopolysaccharides (eps). plankton (freely floating) cells of sulphate-reducing bacteria produce greater quantity of eps than the cells of the biofilm formed on steel. the inducing effect of metal on eps synthesis by sulphate-reducing bacteria has been established. the conten ... | 2004 | 15515905 |
phylogenetic analysis of tce-dechlorinating consortia enriched on a variety of electron donors. | two rapidly fermented electron donors, lactate and methanol, and two slowly fermented electron donors, propionate and butyrate, were selected for enrichment studies to evaluate the characteristics of anaerobic microbial consortia that reductively dechlorinate tce to ethene. each electron donor enrichment subculture demonstrated the ability to dechlorinate tce to ethene through several serial transfers. microbial community analyses based upon 16s rdna, including terminal restriction fragment leng ... | 2005 | 16294874 |
[adhesion of sulphate-reducing bacteria to steel under cathode polarization]. | adhesion of different (as to their corrosion aggression) strains of sulphate-reducing bacteria to steel has been studied under cathode polarization at various potentials: -800, -900, -1000, -1200 mv. it has been established that cathode polarization differently affects the adhesion of strain of sulphate-reducing bacteria with various aggression to steel. correlation between the bacterial strains aggression and the number of cells adhered to metal have been noted. the cells of aggressive strains ... | 2006 | 16686219 |
culture and identification of desulfovibrio spp. from corals infected by black band disease on dominican and florida keys reefs. | black band disease (bbd) of corals is characterized as a pathogenic microbial consortium composed of a wide variety of microorganisms. together, many of these microorganisms contribute to an active sulfur cycle that produces anoxia and high levels of sulfide adjacent to the coral surface, conditions that are lethal to coral tissue. sulfate-reducing bacteria, as sulfide producers, are an important component of the sulfur cycle and the black band community. previous molecular survey studies have s ... | 2006 | 16703774 |
low strength wastewater treatment under low temperature conditions by a novel sulfur redox action process. | the objective of this research is to make a novel wastewater treatment process activated by a sulfur-redox cycle action of microbes in low temperature conditions. this action is carried out by sulfate-reducing bacteria (srb) and sulfur-oxidizing bacteria (sob). the process was comprised of a uasb reactor as pre-treatment and an aerobic downflow hanging sponge (dhs) reactor as post-treatment. as the results of reactor operation, the whole process achieved that over 90% of codcr removal efficiency ... | 2006 | 16749445 |
[microbial community structure in different wastewater treatment processes characterized by single-strand-conformation polymorphism (sscp) technique]. | in order to investigate microbial community structures in different wastewater treatment processes and understand the relationship between the structures and the status of processes, the microbial community diversity, variety and distribution in five wastewater treatment processes were studied by a culture-independent genetic fingerprinting technique single-strand-conformation polymorphism (sscp). the five processes included a denitrification and phosphorus removal bioreactor (n), chinese tradit ... | 2006 | 16881324 |
toxic effects of dissolved heavy metals on desulfovibrio vulgaris and desulfovibrio sp. strains. | biological treatment of metal-containing wastewaters with sulphate-reducing bacteria (srb) is an attractive technique for the bioremediation of this kind of medium. in order to design a suitable engineering process to address this environmental problem, it is crucial to understand the inhibitory effect of dissolved heavy metals on these bacteria. batch studies were carried out to evaluate the toxic effects of several heavy metal ions [cr(iii), cu(ii), mn(ii), ni(ii) and zn(ii)] on two cultures o ... | 2006 | 16386832 |
the effect of decreasing alkalinity on microbial community dynamics in a sulfate-reducing bioreactor as analyzed by pcr-sscp. | pcr-single-strand conformation polymorphism (sscp) and southern blotting techniques were adopted to investigate microbial community dynamics in a sulfate-reducing bioreactor caused by decreasing influent alkalinity. experimental results indicated that the sulfate-removal rate approached 87% in 25 d under the conditions of influent alkalinity of 4000 mg/l (as caco3) and sulfate-loading rate of 4.8 g/(l*d), which indicated that the bioreactor started up successfully. the analysis of microbial comm ... | 2006 | 16989283 |
a 1,1,1-trichloroethane-degrading anaerobic mixed microbial culture enhances biotransformation of mixtures of chlorinated ethenes and ethanes. | 1,1,1-trichloroethane (1,1,1-tca) is a common groundwater pollutant as a result of improper disposal and accidental spills. it is often found as a cocontaminant with trichloroethene (tce) and inhibits some tce-degrading microorganisms. 1,1,1-tca removal is therefore required for effective bioremediation of sites contaminated with mixed chlorinated organics. this study characterized ms, a 1,1,1-tca-degrading, anaerobic, mixed microbial culture derived from a 1,1,1-tca-contaminated site in the nor ... | 2006 | 17056695 |
oil field souring control by nitrate-reducing sulfurospirillum spp. that outcompete sulfate-reducing bacteria for organic electron donors. | nitrate injection into oil reservoirs can prevent and remediate souring, the production of hydrogen sulfide by sulfate-reducing bacteria (srb). nitrate stimulates nitrate-reducing, sulfide-oxidizing bacteria (nr-sob) and heterotrophic nitrate-reducing bacteria (hnrb) that compete with srb for degradable oil organics. up-flow, packed-bed bioreactors inoculated with water produced from an oil field and injected with lactate, sulfate, and nitrate served as sources for isolating several nrb, includi ... | 2007 | 17308184 |
dethiosulfatibacter aminovorans gen. nov., sp. nov., a novel thiosulfate-reducing bacterium isolated from coastal marine sediment via sulfate-reducing enrichment with casamino acids. | a sulfate-reducing enrichment culture originating from coastal marine sediment of the eutrophic tokyo bay, japan, was successfully established with casamino acids as a substrate. a thiosulfate reducer, strain c/g2(t), was isolated from the enrichment culture after further enrichment with glutamate. cells of strain c/g2(t) were non-motile rods (0.6-0.8 microm x 2.2-4.8 microm) and were found singly or in pairs and sometimes in short chains. spores were not formed. cells of strain c/g2(t) stained ... | 2007 | 17911304 |
the geomicrobiology of gold. | microorganisms capable of actively solubilizing and precipitating gold appear to play a larger role in the biogeochemical cycling of gold than previously believed. recent research suggests that bacteria and archaea are involved in every step of the biogeochemical cycle of gold, from the formation of primary mineralization in hydrothermal and deep subsurface systems to its solubilization, dispersion and re-concentration as secondary gold under surface conditions. enzymatically catalysed precipita ... | 2007 | 18043665 |
integrated bacterial process for the treatment of a spent nickel catalyst. | integrated biological processes involving the dissolution and subsequent precipitation have been used for the treatment of the spent material from the hydrogenation of vegetable oil containing a high-level of nickel. our results show that nickel was successfully leached using acidithiobacillus thiooxidans. the percentages of nickel leached using a. thiooxidans were higher than those obtained with dilute sulphuric acid solutions. due to the physical characteristics of the residue, the best result ... | 2008 | 18079054 |
evaluation of the sulfate-reducing bacterial population associated with stored swine slurry. | hydrogen sulfide, produced by sulfate-reducing bacteria (srb), is one of the most potent malodors emitted from anaerobic swine waste storage systems. however, little is known about the prevalence and diversity of srb in those systems. the goals of this study were to evaluate the srb population in swine manure storage systems and to develop quantitative, real-time pcr (qrt-pcr) assays to target four of the srb groups. dissimilatory sulfite reductase (dsr) gene sequences were obtained from swine s ... | 2008 | 18457964 |
growth and cometabolic reduction kinetics of a uranium- and sulfate-reducing desulfovibrio/clostridia mixed culture: temperature effects. | bioremediation of contaminated soils and aquifers is subject to spatial and temporal temperature changes that can alter the kinetics of key microbial processes. this study quantifies temperature effects on the kinetics of an ethanol-fed sulfate-reducing mixed culture derived from a uranium-contaminated aquifer subject to seasonal temperature fluctuations. the mixed culture contains desulfovibrio sp. and a clostridia-like organism. rates of growth, ethanol utilization, decay, and uranium reductio ... | 2008 | 17929318 |
a sulfate-reducing bacterium with unusual growing capacity in moderately acidic conditions. | the use of sulfate-reducing bacteria (srb) is a cost-effective route to treat sulfate- contaminated waters and precipitate metals. the isolation and characterization of a srb strain from an amd in a brazilian tropical region site was carried out. with a moderately acidic ph (5.5), the c.1 strain began its growth and with continued growth, modified the ph accordingly. the strain under these conditions reduced sulfate at the same rate as an experiment performed using an initial ph of 7.0. the dsrb ... | 2008 | 18040868 |
molecular characterization of bacterial communities mineralizing benzene under sulfate-reducing conditions. | the microbial communities of in situ reactor columns degrading benzene with sulfate as an electron acceptor were analyzed based on clone libraries and terminal restriction fragment length polymorphism fingerprinting of pcr-amplified 16s rrna genes. the columns were filled with either lava granules or sand particles and percolated with groundwater from a benzene-contaminated aquifer. the predominant organisms colonizing the lava granules were related to magnetobacterium sp., followed by a phyloty ... | 2008 | 18637040 |
syntrophic growth on formate: a new microbial niche in anoxic environments. | anaerobic syntrophic associations of fermentative bacteria and methanogenic archaea operate at the thermodynamic limits of life. the interspecies transfer of electrons from formate or hydrogen as a substrate for the methanogens is key. contrary requirements of syntrophs and methanogens for growth-sustaining product and substrate concentrations keep the formate and hydrogen concentrations low and within a narrow range. since formate is a direct substrate for methanogens, a niche for microorganism ... | 2008 | 18708519 |
desulfovibrio marinisediminis sp. nov., a novel sulfate-reducing bacterium isolated from coastal marine sediment via enrichment with casamino acids. | to obtain amino acid-utilizing sulfate reducers, enrichment culture was carried out with a medium containing casamino acids and sulfate and inoculated with coastal marine sediment from the eutrophic tokyo bay, japan. a sulfate reducer, designated strain c/l2(t), was isolated from the sulfide-producing enrichment culture after further enrichment with lactate and sulfate by means of the agar shake dilution method. cells of strain c/l2(t) were vibrio-shaped, gram-negative, motile rods (0.7-1.0 mum ... | 2008 | 18842870 |
development and comparison of sybr green quantitative real-time pcr assays for detection and enumeration of sulfate-reducing bacteria in stored swine manure. | to develop and evaluate primer sets targeted to the dissimilatory sulfite reductase gene (dsra) for use in quantitative real-time pcr detection of sulfate-reducing bacteria (srb) in stored swine manure. | 2008 | 19120660 |
effect of oxidized leachate on degradation of lignin by sulfate-reducing bacteria. | municipal solid waste materials (msws) in landfills need a long period of stabilization because lignin compounds in msws and leachate are not readily biodegraded, but inhibit methanogenic metabolism. recirculation of leachate into the landfill offers the potential advantage of increasing the rate of decomposition of organic matter. however, the degradation of lignin by leachate recirculation alone is quite difficult. several recent studies have demonstrated that sulfate-reducing bacteria (srb) w ... | 2009 | 19423591 |
effect of 2,4-dinitrotoluene on the anaerobic bacterial community in marine sediment. | to study the impact of added 2,4-dinitrotoluene (dnt) on the anaerobic bacterial community in marine sediment collected from an unexploded ordnance dumping site in halifax harbour. | 2009 | 19486208 |
culture-independent analysis of desulfovibrios in the human distal colon of healthy, colorectal cancer and polypectomized individuals. | the production of hydrogen sulphide, an end product of metabolism by the sulphate-reducing bacteria (srb) has been cited as a potential aetiological agent in gastrointestinal disease. quantitative pcr (q-pcr) assays to enumerate desulfovibrios from two gastrointestinal disease groups: colorectal cancer (crc) n=27 and polypectomized individuals (pp) n=27, and two healthy control groups, elderly (h1) n=8 and young adults (h2) n=30 was performed. analysis of desulfovibrio sp. diversity using the di ... | 2009 | 19496818 |
[development of mono- and associative cultures of sulphate-reducing bacteria and formation of exopolymeric complex]. | differences were found in development of the monoculture of sulphate-reducing bacteria desulfovibrio sp. kyiv-10 and artificial associations of cultures of sulphate reducing bacteria with heterotrophic satellites under development in a form of biofilm or plankton. correlation between exopolymers synthesis in the biofilm and steel corrosion velocity has been found. glucose, galactose, mannose, xylose, rhibose and nonidentified carbohydrates were found in the composition of exopolymers synthesized ... | 2009 | 19938590 |
composition and dynamics of sulfate-reducing bacteria during the waterflooding process in the oil field application. | the composition and dynamics of sulfate-reducing bacteria (srb) during the waterflooding process of daqing oilfield were investigated in this study. pcr-dgge analysis indicated that the microbial communities were significantly different in each treatment unit, and the dominant members were mainly close to clostridium sp., thauera sp., hydrogenophaga sp., pseudomonas sp., eubacterium sp. and arcobacter sp. however, the members belonging to srb were relatively few and mainly consisted of desulfovi ... | 2010 | 20005702 |
microbial equol production attenuates colonic methanogenesis and sulphidogenesis in vitro. | hydrogen gas produced during colonic fermentation is excreted in breath and flatus, or removed by hydrogen-consuming bacteria such as methanogens and sulphate-reducing bacteria. however, recent research has shown that h2 is also consumed by equol-producing bacteria during the reduction of daidzein into equol. in this study, the interactions between methanogens, sulphate-reducing, and equol-producing bacteria were investigated under in vitro simulated intestinal conditions. in the presence of dai ... | 2010 | 20298796 |
oil phytoremediation potential of hypersaline coasts of the arabian gulf using rhizosphere technology. | the rhizosphere and phyllosphere of the halophyte halonemum strobilaceum naturally inhabiting hypersaline coastal areas of the arabian gulf harbor up to 8.1 x 10(4)g(-1) and 3 x 10(2)g(-1), respectively, of extremely halophilic oil-utilizing microorganisms. such organisms were 14- to 38-fold more frequent in the rhizosphere than in the plant-free soil. frequent genera in the rhizosphere were affiliated to the archaea halobacterium sp. and halococcus sp., the firmicute brevibacillus borstenlensis ... | 2010 | 20303746 |
combined strategy for the precipitation of heavy metals and biodegradation of petroleum in industrial wastewaters. | the precipitation of chromium(iii), copper(ii), manganese(ii) and zinc(ii) by biogenic hydrogen sulfide generated by sulfate-reducing bacteria, desulfovibrio sp., and the degradation of total petroleum hydrocarbons (tph) in the presence of heavy metal by pseudomonas aeruginosa at18 have been carried out. an anaerobic stirred tank reactor was used to generate hydrogen sulfide with desulfovibrio sp. culture and the precipitation of more than 95% of each metal was achieved in 24 h (metal solutions ... | 2010 | 20667656 |
the crystal structure of the [nife] hydrogenase from the photosynthetic bacterium allochromatium vinosum: characterization of the oxidized enzyme (ni-a state). | the crystal structure of the membrane-associated [nife] hydrogenase from allochromatium vinosum has been determined to 2.1 å resolution. electron paramagnetic resonance (epr) and fourier transform infrared spectroscopy on dissolved crystals showed that it is present in the ni-a state (>90%). the structure of the a. vinosum [nife] hydrogenase shows significant similarities with [nife] hydrogenase structures derived from desulfovibrio species. the amino acid sequence identity is ∼ 50%. the bimetal ... | 2010 | 20673834 |
formate-driven growth coupled with h(2) production. | although a common reaction in anaerobic environments, the conversion of formate and water to bicarbonate and h(2) (with a change in gibbs free energy of δg° = +1.3 kj mol(-1)) has not been considered energetic enough to support growth of microorganisms. recently, experimental evidence for growth on formate was reported for syntrophic communities of moorella sp. strain amp and a hydrogen-consuming methanothermobacter species and of desulfovibrio sp. strain g11 and methanobrevibacter arboriphilus ... | 2010 | 20844539 |
desulfovibrio desulfuricans pglb homolog possesses oligosaccharyltransferase activity with relaxed glycan specificity and distinct protein acceptor sequence requirements. | oligosaccharyltransferases (otases) are responsible for the transfer of carbohydrates from lipid carriers to acceptor proteins and are present in all domains of life. in bacteria, the most studied member of this family is pglb from campylobacter jejuni (pglb(cj)). this enzyme is functional in escherichia coli and, contrary to its eukaryotic counterparts, has the ability to transfer a variety of oligo- and polysaccharides to protein carriers in vivo. phylogenetic analysis revealed that in the del ... | 2010 | 21098514 |
[role of exopolymeric substances of corrosion-aggressive bacteria in the biofilm formation on the steel surface]. | it had been done the comparative study of the exopolymeric substances (eps) synthesized by the sulfidogenic microbial community and monocultures of desulfovibrio sp. 10, bacillus subtilis 36 and pseudomonas aeruginosa 27 under various growth models as biofilm and plankton was performed. it was established that biofilm-produced exopolymers contained increased amount of glucose and fucose, while planktonic ones had more amount of mannose and rhamnose. the amount of rhamnose was 24% of the total am ... | 2011 | 21442946 |
Genome sequence of Desulfovibrio sp. A2, a highly copper resistant, sulfate-reducing bacterium isolated from effluents of a zinc smelter at the Urals. | Desulfovibrio sp. A2 is an anaerobic gram-negative sulfate-reducing bacterium with remarkable tolerance to copper. It was isolated from wastewater effluents of a zinc smelter at the Urals. Here, we report the 4.2-Mb draft genome sequence of Desulfovibrio sp. A2 and identify potential copper resistance mechanisms. | 2011 | 22072648 |