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bacillus mortiferus (nov. spec.). 190519866983
[spherophoroses; surgical aspect of the pathogenicity of spherophorus freundi]. 195413190287
[experimental inoculation of the chick embryo with "spherophorus freundi"]. 196013721376
the incidence of herpes zoster antibodies in patients with peripheral facial palsy. 19704323301
dna synthesis in cell-free extracts of a dna polymerase-defective mutant. 19704933688
a study of workers exposed to detergent enzymes. 19714253729
[relationship between delayed hypersensitivity phenomena and cellular immunity in the course of experimental bilharziasis]. 19724640713
identical increases in blood sialic acid of dogs given actinomycin d and dexamethasone. 19724641216
[experimental studies of the possibility of use of stroma-free hemoglobin solution]. 19734694768
corneal collagenases: evidence for zinc metalloenzymes. 19734356811
bacteroidaceae in thromboembolic disease: effects of cell wall components on blood coagulation in vivo and in vitro.the effects of bacteroides sp., fusobacterium mortiferum, bacteroides fragilis, and sphaerophorus necrophorus on various parameters of blood coagulation in vivo and in vitro were determined and compared to the coagulation effects of escherichia coli and salmonella minnesota, wild type and r595. intravenous injection of washed cells, culture filtrate, lipopolysaccharide, or lipid a of the anaerobic gram-negative microorganisms into mice resulted in acceleration of coagulation. lipopolysaccharide ...19734594118
phosphatase activity of anaerobic organisms.anaerobic organisms were tested for phosphatase activity in different ph ranges. several groups of organisms displayed characteristic patterns. bacteroides fragilis, b. melaninogenicus, and b. ruminicola produced phosphatase with strongest activity at ph 8.6. fusobacterium mortiferum was the only species of this genus to show strong hydrolysis. the enzyme was active in both acid and alkaline ranges. the activity of gram-positive organisms was variable, the most active groups being clostridium pe ...19744363556
[the activities of some hydrolases after administration of encephalitogenic protein (author's transl)]. 19744141642
[results of treatment with the herpes simplex antigen lupidon h, resp. lupidon g].the results of treatment with lupidon achieved by 215 dermatologists with reference to a total number of 1059 cases are reported taking into account the controlled evaluation of questionnaires. due to the considerable number of patients and physicians involved in this clinical investigation the presuppositions for objectiveness in respect of statistical evaluation were especially favourable. the results of treatment with lupidon--lupidon h and lupidon g proved to be of equal effectiveness--can b ...1975179231
the possible role of tightly bound adenine nucleotides in oxidative and photosynthetic phosphorylation.the tightly bound nucleotides of the beff-heart mitochondrial atpase are released during cold inactivation followed by ammonium sulfate precipitation. during incubation at 0 degrees c the sedimentation coefficient (s20w) of the atpase first declines from 12.1s to 9s. prolonged incubation or precipitation with ammonium sulfate leads to dissociation of the 9s component into subunits with s20w of 3.5s. the 9s component still bears bound nucleotides which exchange more extensively and rapidly with a ...1975127089
susceptibility of the liver and biliary tract to anaerobic infection in extrahepatic biliary tract obstruction. bacteriological investigations - an experimental study in rabbits.the influence of common duct occlusion on susceptibility to infection with anaerobic gram-negative rods was investigated in rabbits. intravenous inoculation was performed with three b. fragilis strains isolated from human infections and with a stock culture of fusobacterium mortiferum. liver tissue, gallbladder, bile, and blood were investigated. anaerobic culture procedures were based on the use of a 'glove-box' with an oxygen-free atmosphere and pre-reduced, anaerobically sterilized media. ana ...19751188311
neurochemical correlates of alloxan diabetes: brain aminotransferase heterogeneity in the rat. 19751196005
susceptibility of the liver and biliary tract to anaerobic infection in extrahepatic biliary tract obstruction. ii. liver function and morphology of the liver and biliary tract--an experimental study in rabbits.biliary tract infection with anaerobic bacteria (b. fragilis or fusobacterium mortiferum) was produced in rabbits by common duct ligation (c.d.l.) 3 days prior to intravenous bacterial inoculation. animals were investigated 1, 4 or 7 days after inoculation. histopathological investigations included the liver, the common duct, and the gallbladder, while liver function was evaluated by bilirubin, alkaline phosphatase (ap), and l-alanine aminotransferase (gpt) in serum. rabbits with c.d.l. and bili ...19761251134
substitution of serine caused by a recessive lethal suppressor in yeast. 1976178877
comparison of the in vitro bactericidal activity of human serum and leukocytes against bacteroides fragilis and fusobacterium mortiferum in aerobic and anaerobic environments.in aerobic and anaerobic in vitro environments, fusobacterium mortiferum was killed by human leukocytes or serum alone or in combination; clinical isolates of bacteroides fragilis were killed only by leukocytes in the presence of serum.1976965101
survival of anaerobic bacteria during transportation. 1. experimental investigations on the effect of evacuation of atmospheric air by flushing with carbon dioxide and nitrogen.the effect of evacuation of atmospheric air during transportation on recovery of anaerobic bacteria was investigated. evacuation of atmospheric air from glass tubes by flushing with pure carbon dioxide lowered the content of oxygen to about 0.4 per cent. three b. fragilis strains and one strain of fusobacterium mortiferum and of peptostreptococcus anaerobius were investigated. bacterial recovery was determined one hour and 24 hours after evacuation of atmospheric air by pure carbon dioxide and p ...19763089
the effect of mixed phosphatidylcholine liposomes on beef heart cytochrome c oxidase [proceedings]. 197884573
[hepatology and immunology].immunologic diseases of the liver are exogenous mostly initiated by virus or endogenous initiated by autoaggression. all virus-induced kinds of hepatitis are due to an immune response against inocculated hepatocytes. therefore the hepatitis is limited to the period of complete elimination of virus-infected cells. a strong immune response therefore corresponds with an acute and short hepatitis whilest a weak immune response develops a chronic hepatitis. in contrast, autoimmune hepatitis based on ...1978205495
reactivity of platelets with different collagens: overlook. 1978262325
abnormalities of the nuclear envelope in porcine muscle affected with congenital myofibrillar hypoplasia.the nuclear ultrastructure of muscle fibres in congenital myofibrillar hypoplasia of piglets was studied in order to provide more information on the pathological changes present. in the initial stages of the disease, muscle fibres with a small reduction of myofibrils often had an increased number of nuclear pores. in the more advanced stages of the disease, the following nuclear abnormalities were observed in muscle fibres showing an extensive disintegration of myofibrils: 1) large dilations of ...197898911
survival of anaerobic bacteria in common laboratory diluents.the survival of six species of anaerobic bacteria was studied in simple or commercially available diluents. bacteroides fragilis and fusobacterium nucleatum showed excellent survival in all diluents including distilled water. fusobacterium mortiferum survived well in all diluents except water and water supplemented with 0.1% gelatain. clostridium perfringens survived best in phosphate-buffered saline with gelatin. peptococcus asaccharolyticus required gelatin added to the basic diluent, and stre ...1979479359
distribution of myosin isoenzymes among skeletal muscle fiber types.using an immunocytochemical approach, we have demonstrated a preferential distribution of myosin isoenzymes with respect to the pattern of fiber types in skeletal muscles of the rat. in an earlier study, we had shown that fluorescein-labeled antibody against "white" myosin from the chicken pectoralis stained all the white, intermediate and about half the red fibers of the rat diaphragm, a fast-twitch muscle (gauthier and lowey, 1977). we have now extended this study to include antibodies prepare ...197990047
polyribonucleotide inhibition of ribonucleic acid directed deoxyribonucleic acid polymerase of mouse mammary tumor (type b) virus and simian sarcoma (type c) virus. 197990331
[animal experimental studies on the volume expanding effect of hydroxyethyl starch 40000 in the dog acute hemorrhagic shock].after acute hemorrhagic shock in dogs the hemodynamics of hydroxyethyl starch (mw: 40,000) are studied. the experiment shows that this plasma expander has a good efficiency for 3 to 4 hours. the indications of application of this colloidal solution are discussed.197990651
purification of a new high activity form of glucose-6-phosphate dehydrogenase from rat liver and the effect of enzyme inactivation on its immunochemical reactivity.a new form of cytoplasmic glucose-6-phosphate dehydrogenase (e.c.1.1.1.49) was purified from rat liver by protamine sulfate precipitation, ammonium sulfate fractionation, ion exchange chromatography with diethylaminoethyl cellulose, and affinity chromatography with cibacron blue agarose and nadp agarose. this form of the enzyme has a specific activity of over 600 units/mg of protein and gives essentially a single band by polyacrylamide gel electrophoresis. the form of the enzyme isolated by this ...197990677
binding of purified, soluble major histocompatibility complex polypeptide chains onto isolated t-cell receptors. i. reactivity against allo- and self-determinants.in this study, we tried to get information about the fine antigen-binding ability of purified, soluble, idiotype-positive t-cell receptor molecules. lewis anti-da t-cell receptors were purified from normal lewis serum by the use of anti-idiotypic immunosorbent and sodium dodecyl sulfate-polyacrylamide gel, and were coupled to cyanogen bromide-activated sepharose 4b. in parallel, lewis anti-da, lewis anti-bn, and da anti-lewis alloantibody immunosorbents were prepared. the major ag-b chain (44,00 ...197991653
gram-negative anaerobic bacilli: their role in infection and patterns of susceptibility to antimicrobial agents. ii. little-known fusobacterium species and miscellaneous genera.twenty infrequently reported species of gram-negative anaerobic bacilli other than fusobacterium nucleatum, fusobacterium necrophorum, and members of the genus bacteroides were studied with regard to their role in infection and their susceptibility to antimicrobial agents. in addition, the literature regarding the recovery of these organisms from both the normal flora and infections of humans was reviewed. during a six-year period at the wadsworth clinical anaerobic bacteriology research laborat ...19817025153
predicting the stability of freeze-dried fusobacterium mortiferum proficiency testing samples by accelerated storage tests. 19817307532
biotransformation of 1-nitropyrene in intestinal anaerobic bacteria.mutagenic nitroaromatic compounds have recently been found in photocopies, urban atmosphere, automobile exhaust and wastewater. 1-nitropyrene (1-np) is readily formed when pyrene, ubiquitous in the environment, is exposed to nitrogen dioxide in the urban atmosphere or in automobile exhaust, and is highly mutagenic, inducing 449 his+ revertants/plate/nmol from salmonella typhimurium strain ta98 in the absence of s9 fraction in the salmonella-microsome test. it is possible to swallow sputum or som ...19827167066
cellular fatty acid and soluble protein profiles of oral fusobacteria.we compared the cellular fatty acid and protein content of 43 strains of oral fusobacteria isolated from patients with chronic gingivitis, acute necrotizing ulcerative gingivitis, and juvenile and adult periodontitis, as well as from the stump-tailed macaque, to that of eight reference strains of oral and non-oral fusobacterium species. a gas-liquid chromatographic examination of trimethylsilyl derivatives of the fatty acid methyl esters revealed n14:0, 3-oh-14:0, n16:0, 16:1, 3-oh-16:0, n18:0, ...19836581193
activation of hageman factor by lipopolysaccharides of bacteroides fragilis, bacteroides vulgatus, and fusobacterium mortiferum.preincubation of lipopolysaccharides (lps) of bacteroides fragilis, bacteroides vulgatus, and fusobacterium mortiferum with purified human hageman factor (hf) followed by addition of purified human prekallikrein resulted in the activation of hf, as measured by the generation of kallikrein using the tripeptide substrate n-alpha-benzoyl-l-proline-l-phenylalanine-l-arginine-4-nitroanilide. addition of purified hf, which had been preincubated with the lps, to hf-deficient plasma resulted in the acce ...19846718941
therapeutic effect of cefotetan in an experimental local infection with facultative and obligate anaerobes in granuloma pouches in rats.the therapeutic activities of cefotetan against experimentally induced infections with escherichia coli. serratia marcescens, bacteroides fragilis or fusobacterium mortiferum in the granuloma pouches of rats were compared with those of cefmetazole and cefazolin. of these antibiotics, cefotetan showed the greatest therapeutic effect against the four bacterial infections after a single intramuscular administration. cefotetan was found to give much higher pouch exudate concentrations than cefmetazo ...19846368517
lack of constitutive beta-glucosidase (esculinase) in the genus fusobacterium.esculin has been incorporated into both a medium and test with 20% bile for many years to differentiate bacteroides from fusobacterium organisms. after 24 to 48 h, all members of the bacteroides fragilis group grow in 20% bile and hydrolyze esculin. fusobacterium mortiferum can both grow in bile and hydrolyze esculin, thus limiting the use of the bile-esculin medium and test. the hypothesis that constitutive esculinase (beta-glucosidase) could differentiate bacteroides from fusobacterium organis ...19853930563
chlamydial proctitis?chlamydia trachomatis was isolated from 21 (7%) of 309 specimens obtained in october 1982 from the rectum of homosexual men undergoing proctoscopy. during the same period neisseria gonorrhoeae was isolated from 12 (3%) of 454 specimens obtained similarly. the clinical features of patients infected with each of these micro-organisms were compared with those of an uninfected group of homosexual men. no characteristic diagnostic features were noted in the infected men.19853936777
[ability of a phosphocreatine-myofibrillar creatine kinase system to prevent the rigor tension of myocardial fibers].in the calcium-free medium the egta-treated rat myocardial fibres developed rigor tension dependent on the concentration of mgatp in the bathing solution: half-maximal tension was recorded at 2.5 mm mgatp and the maximal tension at 0.1 mm. however, in the presence of 15 mm phosphocreatine without added creatine kinase a decrease of mgatp concentration to 0.1 mm did not result in any development of rigor tension. in the presence of mgadp phosphocreatine decreased rigor tension more rapidly and to ...19853986231
hypothermia produced by tributyl s,s,s-phosphorotrithioate (def).tributyl s,s,s-phosphorotrithioate (def) produces profound hypothermia in rats, mice and guinea pigs by inhibition of thermogenesis. its actions on heat conservation and motor control are, however, minimal. it is effective against both shivering and non-shivering thermogenesis and completely blocks the increase in body temperature evoked by anterior hypothalamic stimulation. a number of other measures indicated that this is unlikely to be due to a lack of peripheral thermogenic capacity: thus pl ...19853994512
regulation of net bicarbonate transport in rabbit cortical collecting tubule by peritubular ph, carbon dioxide tension, and bicarbonate concentration.the effects of changes in peritubular ph, carbon dioxide tension (pco2), and hco3- concentration on net hco3- transport was examined in in vitro perfused cortical collecting tubules (ccts) from unpretreated new zealand white rabbits. lowering peritubular hco3- concentration and ph by reciprocal replacement of hco3- with cl-, significantly stimulated net hco3- absorption. lowering peritubular hco3- concentration and ph, by substitution of hco3- with gluconate, while keeping cl- concentration cons ...19863084564
anaerobic septicemia secondary to fusobacterium mortiferum.a case of anaerobic sepsis associated with fusobacterium mortiferum is reported. blood cultures from a 60-year-old man with type ii diabetes mellitus, hypertension, severe atherosclerotic cardiovascular disease, and renal insufficiency revealed on a gramstained smear highly pleomorphic gram-negative bacilli with bizarre forms and round bodies. growth of the organism on nonselective anaerobic media and analysis of its pattern produced results characteristic of fusobacterium mortiferum.19863712472
bvd virus infection and embryo transfer. 19863716157
characterization of the protein expressed in escherichia coli by a recombinant plasmid containing the bacillus megaterium cytochrome p-450bm-3 gene.in two previous reports (narhi lo, fulco aj, j. biol. chem. 261: 7160-7169, 1986; ibid., 262: 6683-6690, 1987) we described the characterization of a catalytically self-sufficient 119,000-dalton p-450 cytochrome that was induced by barbiturates in bacillus megaterium. in the presence of nadph and o2, this polypeptide (cytochrome p-450bm-3) catalyzed the hydroxylation of long-chain fatty acids without the aid of any other protein. the gene encoding this unique monooxygenase was cloned into escher ...19883131661
exogenous fungal endophthalmitis.the authors report 19 cases of exogenous fungal endophthalmitis managed between 1969 and 1986. these developed after ocular surgery (5 cases), after trauma (6 cases), by intraocular spread from fungal keratitis (4 cases), after therapeutic keratoplasty for keratitis (3 cases), and by spontaneous infection of a filtration bleb (1 case). the diagnosis of endophthalmitis was made from 3 days to 4 months after surgery or trauma. in patients with preexisting keratitis, endophthalmitis was noted 2 wee ...19883257818
the preparation and characterization of cr(iii) and co(iii) complexes of gdp and gtp and their interactions with avian phosphoenolpyruvate carboxykinase.the exchange inert coordination complexes, cr(h2o)4gdp, cr(h2o)4gtp, cr(nh3)4gdp, cr(nh3)4gtp, co(nh3)4gdp, and co(nh3)4gtp have been synthesized and characterized. the lambda and delta coordination isomers of cr(h2o)4gdp, cr(nh3)4gdp, and the four cr(h2o)4gtp isomers have been separated by reverse phase hplc and characterized by their cd spectra. while the isomers of co(nh3)4gtp have not been successfully separated, 31p nmr spectroscopy reveals the presence of the lambda and delta forms. the co ...19883346664
requirement of hormone for thermal conversion of the glucocorticoid receptor to a dna-binding state.a central question arising from the model of eukaryotic gene regulation by steroid hormone receptors is whether or not proteins represent pre-existing gene regulatory proteins that are activated on exposure to the extracellular signal. it has been generally believed that the ligand-binding of steroid hormone receptors triggers an allosteric change in receptor structure, manifested by an increased affinity of the receptor for dna in vitro and nuclear target elements in vivo, as monitored by nucle ...19883374615
ecology of colorado tick fever. 19882849373
antiviral chemoprophylaxis for cytomegalovirus infection. 19882849602
comparison of fecal microflora of elderly persons in rural and urban areas of japan.the fecal microflora of 15 healthy elderly persons with a median age of 84 years in a rural area whose inhabitants tend to be long-lived (yuzurihara-area, uenohara, yamanashi prefecture) was compared with the microflora of individuals with a median age of 68 years in an urban area (tokyo). the diet of the elderly persons in the yuzurihara area is characterized by a high intake of dietary fiber. total numbers of anaerobic bacteria were significantly smaller in the elderly persons in the yuzurihar ...19892547333
interleukin-1 and interleukin-1 inhibitor production by human adherent cells stimulated with periodontopathic bacteria.this study examined the effect of the putative periodontopathic bacteria bacteroides gingivalis and fusobacterium nucleatum on the production of interleukin-1 (il-1) and il-1 inhibitors by human plastic-adherent mononuclear cells from normal donors. fusobacterium mortiferum was used as a non-oral, non-pathogenic control organism. unstimulated adherent cells spontaneously secreted an il-1 inhibitor, whereas stimulation with b. gingivalis induced the synthesis and secretion of il-1. with both fuso ...19892624558
effects of periodontopathic bacteria on il-1 and il-1 inhibitor production by human polymorphonuclear neutrophils.the effect of heat-killed periodontopathic bacteria on the production of interleukin-1 (il-1) and an il-1 inhibitor by human polymorphonuclear neutrophils (pmn's) was examined. peripheral blood was obtained from 18 healthy volunteers and the pmn's were separated using dextran sedimentation and ficoll-paque density gradient centrifugation. the pmn's (5 x 10(5) cells/well) were cultured in serum-free media with or without heat-killed periodontopathic bacteria. four gram-negative periodontopathic b ...19892640313
5s ribosomal ribonucleic acid sequences in bacteroides and fusobacterium: evolutionary relationships within these genera and among eubacteria in general.the 5s ribosomal ribonucleic acid (rrna) sequences were determined for bacteroides fragilis, bacteroides thetaiotaomicron, bacteroides capillosus, bacteroides veroralis, porphyromonas gingivalis, anaerorhabdus furcosus, fusobacterium nucleatum, fusobacterium mortiferum, and fusobacterium varium. a dendrogram constructed by a clustering algorithm from these sequences, which were aligned with all other hitherto known eubacterial 5s rrna sequences, showed differences as well as similarities with ...198911542181
in-vitro susceptibilities of bacteroides gracilis, fusobacterium mortiferum and f. varium to 17 antimicrobial agents. 19902211439
beta-lactamase production and susceptibilities to amoxicillin, amoxicillin-clavulanate, ticarcillin, ticarcillin-clavulanate, cefoxitin, imipenem, and metronidazole of 320 non-bacteroides fragilis bacteroides isolates and 129 fusobacteria from 28 u.s. centers.beta-lactamase production (nitrocefin disk method) and agar dilution susceptibility of amoxicillin, amoxicillin-clavulanate, ticarcillin, ticarcillin-clavulanate, cefoxitin, imipenem, and metronidazole were determined for 320 bacteroides species (not bacteroides fragilis group) and 129 fusobacteria from 28 u.s. centers. overall, 64.7% of bacteroides species and 41.1% of fusobacteria were beta-lactamase positive. among the bacteroides species, positivity rates were highest for b. bivius (85.0%), ...19902221864
the stafford outbreak of legionnaires' disease.a large outbreak of legionnaires' disease was associated with stafford district general hospital. a total of 68 confirmed cases was treated in hospital and 22 of these patients died. a further 35 patients, 14 of whom were treated at home, were suspected cases of legionnaires' disease. all these patients had visited the hospital during april 1985. epidemiological investigations demonstrated that there had been a high risk of acquiring the disease in the out patient department (opd), but no risk i ...19902347381
intrageneric relationships of members of the genus fusobacterium as determined by reverse transcriptase sequencing of small-subunit rrna.the phylogenetic interrelationships of 14 members of the genus fusobacterium were investigated by performing a comparative analysis of the 16s rrna sequences of these organisms. the sequence data revealed considerable intrageneric heterogeneity. the four species fusobacterium nucleatum (including f. nucleatum subsp. nucleatum, f. nucleatum subsp. polymorphum, "f. nucleatum subsp. fusiforme," and "f. nucleatum subsp. animalis"), fusobacterium alocis, fusobacterium periodonticum, and fusobacterium ...19911715737
biological functions of the water-insoluble fraction of mouse seminal vesicle fluid. i. suppression of the blastogenic response of lymphocytes.the effects of the water-insoluble fraction of mouse seminal vesicle fluid (wif-svf) on lymphocytes was investigated to clarify its role in reproductive immunity. wif-svf inhibited the blastogenic response of t-cells to concanavalin-a (con-a), but it did not inhibit the blastogenic response of b-cells to lipopolysaccharide (lps). pretreatment of splenocytes with wif-svf did not suppress the blastogenic response of splenocytes to con-a when treated cells were washed prior to culture. wif-svf did ...19911906049
sugar metabolism by fusobacteria: regulation of transport, phosphorylation, and polymer formation by fusobacterium mortiferum atcc 25557.strains of eight fusobacterium species differed in the ability to use sugars as energy sources for growth. for fusobacterium russii atcc 25533, f. gonidiaformans atcc 25563, and f. nucleatum atcc 10953 (except for fructose), growth was marginal to poor on all of the sugars tested. other species displayed reasonable growth on glucose, fructose, mannose, and galactose, and two strains of f. mortiferum (atcc 25557 and atcc 9817) grew well on six of the sugars tested, including sucrose and maltose. ...19911937813
analyzing phenotypic correlations in studies with selected lines. 19921317160
sucrose fermentation by fusobacterium mortiferum atcc 25557: transport, catabolism, and products.studies of sucrose utilization by fusobacterium mortiferum atcc 25557 have provided the first definitive evidence for phosphoenolpyruvate-dependent sugar:phosphotransferase activity in the family bacteroidaceae. the phosphoenolpyruvate-dependent sucrose:phosphotransferase system and the two enzymes required for the dissimilation of sucrose 6-phosphate are induced specifically by growth of f. mortiferum on the disaccharide. monomeric sucrose 6-phosphate hydrolase (m(r), 52,000) and a dimeric atp- ...19921533618
numerical taxonomy of bacteroides and other genera of gram-negative anaerobic rods.a numerical taxonomy was performed on 157 cultures (141 different strains) of species of bacteroides, polyphyromonas, prevotella [not prevotella (labroue, 1976)] and fusobacterium. isolates were each tested for 111 phenotypic characters which included possession of constitutive enzymes, fermentation of specific carbohydrates, gas chromatographic analysis of metabolic end-products and of cellular carboxylic acid composition. computation of similarity coefficients was followed by a single-linkage ...19921593964
inactivation of anaerobic bacteria by various photosensitized porphyrins or by hemin.the photodynamic effects of deuteroporphyrin (dp), hematoporphyrin derivative (hpd), hematoporphyrin (hp), or protoporphyrin (pp) on a variety of anaerobic microorganisms were examined in this study. the majority of the species, among the 350 strains tested, were inhibited by concentrations of < or = 2.5 micrograms/ml of light-activated dp. species found to be resistant to this treatment included bilophila wadsworthia, fusobacterium mortiferum, fusobacterium varium, and bacteroides gracilis. the ...19947765091
in vitro activity of du-6859a against anaerobic bacteria.the activity of a new quinolone agent, du-6859a, against 330 strains of anaerobic bacteria was determined by using the national committee for clinical laboratory standards-approved wadsworth brucella laked blood agar method; the activity of du-6859a was compared with those of amoxicillin-clavulanate (2:1), chloramphenicol, ciprofloxacin, clindamycin, fleroxacin, imipenem, lomefloxacin, metronidazole, sparfloxacin, and temafloxacin. du-6859a and chloramphenicol inhibited all of the isolates at co ...19947840599
in-vitro activity of clinafloxacin (ci-960) and pd 131628-2 against anaerobic bacteria.the antimicrobial activities of two new quinolones, ci-960 and pd 131628-2 were determined against 339 strains of anaerobic bacteria and compared to cefoxitin, imipenem and metronidazole. the nccls-approved wadsworth agar dilution technique with brucella-lysed blood agar was used throughout the study. breakpoints of the new quinolones are 2 mg/l, and breakpoints for cefoxitin, imipenem and metronidazole are 32, 8 and 16 mg/l, respectively. ci-960 displayed excellent activity, inhibiting all stra ...19947868409
fusobacterial infections in children.a total of 243 strains of fusobacteria species was recovered from 226 of 1399 (16%) specimens obtained from 213 children. the strains included 65 (27%) fusobacterium sp., 144 (59%) fusobacterium nucleatum, 25 (10%) fusobacterium necrophorum, five (2%) fusobacterium varium, three (1%) fusobacterium mortiferum, and one (0.4%) fusobacterium gonidiaformans. most fusobacteria species were recovered from patients with abscesses (100), aspiration pneumonia (24), paronychia (15), bites (14), chronic sin ...19948034995
effect of co2 on susceptibilities of anaerobes to erythromycin, azithromycin, clarithromycin, and roxithromycin.the oxyrase agar dilution method (oxyrase, inc., mansfield, ohio), which provides an anaerobic environment without added co2, was compared with the reference agar dilution method recommended by the national committee for clinical laboratory standards (anaerobic chamber with 10% co2) to test the susceptibilities of 302 gram-negative and gram-positive anaerobes to erythromycin, azithromycin, clarithromycin, and roxithromycin. for erythromycin, the overall mic for 50% of isolates tested (mic50) was ...19948192445
phosphoenolpyruvate-dependent maltose:phosphotransferase activity in fusobacterium mortiferum atcc 25557: specificity, inducibility, and product analysis.phosphoenolypyruvate-dependent maltose:phosphotransferase activity was induced in cells of fusobacterium mortiferum atcc 25557 during growth on maltose. the disaccharide was rapidly metabolized by washed cells maintained under anaerobic conditions, but fermentation ceased immediately upon exposure of the cell suspension to air. coincidentally, high levels of a phosphorylated derivative accumulated within the cells. chemical and enzymatic analyses, in conjunction with data from 1h, 13c, and 31p n ...19948195080
[displaced teeth and anterior crowding]. 1994284428
purification from fusobacterium mortiferum atcc 25557 of a 6-phosphoryl-o-alpha-d-glucopyranosyl:6-phosphoglucohydrolase that hydrolyzes maltose 6-phosphate and related phospho-alpha-d-glucosides.6-phosphoryl-o-alpha-d-glucopyranosyl:6-phosphoglucohydrolase (6-phospho-alpha-glucosidase) has been purified from fusobacterium mortiferum atcc 25557. p-nitrophenyl-alpha-d-glucopyranoside 6-phosphate (pnp alpha glc6p) served as the chromogenic substrate for detection and assay of enzyme activity. the o2-sensitive, metal-dependent phospho-alpha-glucosidase was stabilized during purification by inclusion of dithiothreitol and mn2+ ion in chromatography buffers. various 6-phosphoryl-o-alpha-linke ...19957730284
phospho-beta-glucosidase from fusobacterium mortiferum: purification, cloning, and inactivation by 6-phosphoglucono-delta-lactone.6-phosphoryl-beta-d-glucopyranosyl:6-phosphoglucohydrolase (p-beta-glucosidase, ec 3.2.1.86) has been purified from fusobacterium mortiferum. assays for enzyme activity and results from western immunoblots showed that p-beta-glucosidase (mr, 53,000; pi, 4.5) was induced by growth of f. mortiferum on beta-glucosides. the novel chromogenic and fluorogenic substrates, p-nitrophenyl-beta-d-glucopyranoside-6-phosphate (pnpbetaglc6p) and 4-methylumbelliferyl-beta-d-glucopyranoside-6-phosphate (4mubeta ...19979045824
antianaerobic activity of the ketolide ru 64004 compared to activities of four macrolides, five beta-lactams, clindamycin, and metronidazole.agar dilution methodology (with added oxyrase in the case of the macrolide group to allow incubation without added co2) was used to compare the activity of ru 64004, a new ketolide, with the activities of erythromycin, azithromycin, clarithromycin, roxithromycin, clindamycin, amoxicillin with and without clavulanate, piperacillin with and without tazobactam, metronidazole, and imipenem against 379 anaerobes. overall, ru 64004 yielded an mic at which 50% of the isolates are inhibited (mic50) of 1 ...19979145865
6-phospho-alpha-d-glucosidase from fusobacterium mortiferum: cloning, expression, and assignment to family 4 of the glycosylhydrolases.the fusobacterium mortiferum malh gene, encoding 6-phospho-alpha-glucosidase (maltose 6-phosphate hydrolase; ec 3.2.1.122), has been isolated, characterized, and expressed in escherichia coli. the relative molecular weight of the polypeptide encoded by malh (441 residues; mr of 49,718) was in agreement with the estimated value (approximately 49,000) obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the enzyme purified from f. mortiferum. the n-terminal sequence of the mal ...19979209025
time-kill study of the activity of trovafloxacin compared with ciprofloxacin, sparfloxacin, metronidazole, cefoxitin, piperacillin and piperacillin/tazobactam against six anaerobes.a time-kill method was developed to examine the killing kinetics of trovafloxacin, ciprofloxacin, sparfloxacin, metronidazole, cefoxitin, piperacillin and piperacillin/tazobactam against one strain each of bacteroides fragilis, bacteroides thetaiotaomicron, prevotella melaninogenica, fusobacterium mortiferum, peptostreptococcus magnus and clostridium perfringens. solutions and suspensions were prepared inside an anaerobic glove box, using syringes and prereduced broth. bottles were then incubate ...19979222066
comparative antianaerobic activities of the ketolides hmr 3647 (ru 66647) and hmr 3004 (ru 64004).hmr 3647 (ru 66647) and hmr 3004 (ru 64004), two ketolides, had mics at which 50% of the strains are inhibited (mic50s) of 0.06 to 0.125 microg/ml and mic90s of 16.0 microg/ml against 352 anaerobes. mic50s and mic90s of erythromycin, azithromycin, clarithromycin, and roxithromycin were 0.5 to 2.0 microg/ml and 32.0 to >64.0 microg/ml, respectively. hmr 3647 and hmr 3004 were more active against non-bacteroides fragilis-group anaerobes (other than fusobacterium mortiferum, fusobacterium varium, a ...19979303406
coaggregation of candida albicans with oral fusobacterium species.nine strains of oral fusobacterium were examined for their ability to coaggregate in vitro with four strains of the oral yeast. candida albicans. all of the fusobacterium nucleatum strains and fusobacterium periodontium and fusobacterium sulci coaggregated to various degrees with all of the candida strains. fusobacterium alocis, fusobacterium mortiferum and fusobactrium simiae strains did not coaggregate with any of the candida strains. exposure of the coaggregating fusobacterium strains but not ...19979467403
growth hormone release after synthetic 1-24 acth: effects of estrogen and sex.recent reports have demonstrated that growth hormone (gh) is inconsistently released after intravenous administration of synthetic 1-24 acth. this study was designed to evaluate the role of estrogens in mediating this response. 250 mug of synthetic 1-24 acth were administered to 8 men in 12 studies and 6 women in 11 studies. none of the men exhibited a rise in gh after 1-24 acth, while the women exhibited a significant increase. pretreatment of 5 of these men with 100 mug of ethinyl estradiol da ...1997163846
[c-peptide, igg-insulin antibodies, island cell antibodies and immune response genes in diabetes mellitus]. 1998105488
subgingival microbiota of shallow periodontal pockets in individuals after head and neck irradiation.this study aimed at investigating the subgingival plaque microorganisms of shallow pockets (< or = 5 mm) in subjects who previously received irradiation in the head and neck region for treatment of nasopharyngeal carcinoma. direct microscopy and anaerobic culture were used. subgingival paper point samples were taken from 6 tooth-sites (one/sextant) per subject for direct microscopy (n = 108). another set of paper points was taken from the deepest of the previously selected sites (one per subject ...19989573815
helicobacter pylori adheres selectively to fusobacterium spp.helicobacter pylori strains atcc 43504 and atcc 43629 were tested for their ability to coaggregate with 79 strains of bacteria representing 16 genera. all except two of the strains were of human origin, and most of the strains were isolated from the oral cavity. the helicobacters failed to coaggregate with all strains except the fusobacteria. several coaggregations were partially or completely inhibited by lactose. strong coaggregation was seen with each of four subspecies of fusobacterium nucle ...19989573823
subgingival microflora associated with nifedipine-induced gingival overgrowth.the purpose of this study was to examine the composition of subgingival plaque of 140 periodontal lesions in 35 patients with cardiovascular disorders who were administered nifedipine and manifested nifedipine-induced gingival overgrowth (go). age was inversely associated with the go. plaque index and bleeding index showed a significant association with go, while nifedipine dosage and duration of nifedipine therapy were not found to be significant predictors of go. the gingival inflammation as e ...19989660335
the gene glva of bacillus subtilis 168 encodes a metal-requiring, nad(h)-dependent 6-phospho-alpha-glucosidase. assignment to family 4 of the glycosylhydrolase superfamily.the gene glva (formerly glv-1) from bacillus subtilis has been cloned and expressed in escherichia coli. the purified protein glva (449 residues, mr = 50,513) is a unique 6-phosphoryl-o-alpha-d-glucopyranosyl:phosphoglucohydrolase (6-phospho-alpha-glucosidase) that requires both nad(h) and divalent metal (mn2+, fe2+, co2+, or ni2+) for activity. 6-phospho-alpha-glucosidase (ec 3.2.1.122) from b. subtilis cross-reacts with polyclonal antibody to maltose 6-phosphate hydrolase from fusobacterium mo ...19989765262
isolation of clostridium tetani from anaerobic empyema.we report the isolation of clostridium tetani (along with fusobacterium mortiferum) from empyema pus. the patient, a 68 year old retired farmer from rural nsw, had recently undergone cholecystectomy, had heart failure and developed an empyema. he improved after drainage of the empyema and penicillin therapy, but died suddenly during convalescence.19989839318
effect on bone of withdrawal of oestrogen therapy. 199989300
duct obstruction with an acrylate glue for treatment of chronic alcoholic pancreatitis.six patients have been treated for chronic alcoholic pancreatitis by total obstruction of the pancreatic duct. in each instance the pancreatic duct was of near normal calibre, and any other procedure would have involved pancreatic resection. there has been little morbidity, and three of six cases have had complete pain relief.199989560
[comparison of the cytomorphological classification with the cytochemical differentiation of acute lymphatic leukemia in children].in 100 children with acute lymphatic leukaemia the cytomorphological subclassification of the pathological cell type was made according to mathé and the french-american-british co-operative group (fab). in addition, all cases of leukaemia were differentiated according to their cytochemical type. lymphoblasts from 10 cases of leukaemia could be subclassified immunologically. from 71 children will all the survival rates of those cases of leukaemia subclassified cytomorphologically and the cytochem ...199991554
continuous subcutaneous insulin infusion or subcutaneous insulin injections. 199989550
beta-lactamase inhibitors--a novel approach. 199989565
clomipramine and clonazepam in cataplexy. 200091833
synergic activity, for anaerobes, of trovafloxacin with clindamycin or metronidazole: chequerboard and time-kill methods.chequerboard titrations were used to test the activity of trovafloxacin, alone and in combination with clindamycin or metronidazole, against 156 gram-positive or gram-negative anaerobes, including 47 bacteroides fragilis group, 36 prevotella spp., 26 fusobacteria, 21 peptostreptococci and 26 clostridia. mic50/mic90 values (mg/l) of each drug alone against all 156 strains were: trovafloxacin, 0.5/1; clindamycin, 0.25/2; metronidazole, 1/2. synergy (fic indices </= 0.5) was seen in two strains wit ...200010797085
a fusobacterium mortiferum strain produces a bacteriocin-like substance(s) inhibiting salmonella enteritidis.seven strictly anaerobic strains showing anti-salmonella enteritidis activity were isolated from poultry caecal contents. among them, the most inhibitory one, a strain of fusobacterium mortiferum, called fm1025, was selected. biochemical tests, showing the proteinaceous structure of the antagonist(s) produced, indicated that the strain fus. mortiferum fm1025 synthesized (a) bacteriocin-like compound(s) active against salm. enteritidis. among the other strains tested, both gram-negative and gram- ...200010972711
comparative in vitro activities of abt-773 against 362 clinical isolates of anaerobic bacteria.the activity of abt-773, a novel ketolide antibiotic, against clinical isolates of anaerobic bacteria was determined and compared to the activities of other antimicrobial agents. mics at which 90% of isolates were inhibited (mic(90)s) were </=0.06 microg/ml for actinomyces spp., clostridium perfringens, peptostreptococcus spp., propionibacterium spp., and porphyromonas spp. the mic(50)s and mic(90)s were </=0.06 and >32 microg/ml, respectively, for eubacterium spp., lactobacillus spp., clostridi ...200111120995
in vitro activity of telithromycin (hmr 3647) against 502 strains of anaerobic bacteria.in a previous study, we compared hmr 3004 with azithromycin, clarithromycin, erythromycin and roxithromycin against 502 anaerobic bacteria using nccls-approved procedures. this report extends this study by reporting the activity of telithromycin (hmr 3647) against these strains. telithromycin inhibited 10% of bacteroides fragilis, 50% of other b. fragilis group organisms and 93% of other bacteroides spp. telithromycin inhibited all porphyromonas spp. and 98% of prevotella spp. activity against b ...200111266423
fetal heart-rate monitoring. 200190290
discovery of an inherited bisatellited metacentric microchromosome in amniotic cell culture.the identification is reported of an extra bisatellited metacentric microchromosome in amniotic cell culture from the third pregnancy of an identical twin (amniocentesis being performed because of age), and its subsequent finding in the maternal parent as an inherited familial marker. the carriers of the microchromosome are all clinically normal and the parents opted for continuation of pregnancy. only one other report was found in the literature of a similar microchromosome detected in amniotic ...200190568
the effects of religious backgrounds on emotional problems. 2002126985
[clinical aspects of uveitis of the climacteric]. 2002137446
[retrospective analysis of fusobacterium associated infections; experience at aomori prefectural hospital with 108 cases from 1995 to 1999].we experienced 108 cases of fusobacterium associated infections, including otolaryngeal, oral, pleuropulmonary, intraabdominal, skin and soft tissue infections, at aomori prefectural hospital during the 5 year-period from 1995 to 1999. a total of 433 organisms, included 113 fusobacterium spp. (80 fusobacterium nucleatum, 18 fusobaterium necrophorum, 5 fusobacterium varium, 4 fusobacterium mortiferum, 6 fusobacterium spp.), were recovered with an average of 4.0 organisms per case of the 108 cases ...200211852471
metabolism of sucrose and its five isomers by fusobacterium mortiferum.fusobacterium mortiferum utilizes sucrose [glucose-fructose in alpha(1-->2) linkage] and its five isomeric alpha-d-glucosyl-d-fructoses as energy sources for growth. sucrose-grown cells are induced for both sucrose-6-phosphate hydrolase (s6ph) and fructokinase (fk), but the two enzymes are not expressed above constitutive levels during growth on the isomeric compounds. extracts of cells grown previously on the sucrose isomers trehalulose alpha(1-->1), turanose alpha(1-->3), maltulose alpha(1-->4 ...200211882720
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