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serological analysis of butyrivibrio from the bovine rumen.margherita, s. s. (university of california, davis) and r. e. hungate. serological analysis of butyrivibrio from the bovine rumen. j. bacteriol. 86:855-860. 1963.-the cultural and fermentation characteristics of a number of strains of butyrivibrio fibrisolvens isolated from the bovine rumen of cattle from different areas were determined, and the strains were subjected to serological analysis by the techniques of agglutination, immunodiffusion, and indirect hemolysis. in general, the results of t ...196314066485
biohydrogenation of unsaturated fatty acids by rumen bacteria.polan, c. e. (north carolina state of the university of north carolina, raleigh), j. j. mcneill, and s. b. tove. biohydrogenation of unsaturated fatty acids by rumen bacteria. j. bacteriol. 88:1056-1064. 1964.-a simple, rapid, specific assay for the biohydrogenation of unsaturated fatty acids was developed. with this assay, it was shown that washed suspensions of mixed rumen bacteria hydrogenate linoleic and oleic acids. butyrivibrio fibrisolvens, a common rumen bacterium, is capable of hydrogen ...196414219019
[description of triatoma pseudomaculata, a new species of triatominae of sobral, ceará (hemiptera, reduviidae)]. 19644959506
enzymic activities of lipofuscin age pigments: comparative histochemical and biochemical studies. 19654284732
the biohydrogenation of alpha-linolenic acid and oleic acid by rumen micro-organisms.1. alpha-[u-(14)c]linolenic acid was incubated with the rumen contents of sheep and the metabolic products were characterized by thin-layer chromatography, gas-liquid chromatography and absorption spectroscopy in the ultraviolet and infrared. 2. a tentative scheme for the biohydrogenation route to stearic acid is presented. the main pathway is through diconjugated cis-cis-cis-octadecatrienoic acid, non-conjugated trans-cis (cis-trans)-octadecadienoic acid and trans-octadecenoic acid, but other p ...19664287407
enzymatic studies of pure cultures of rumen microorganisms.joyner, a. e., jr. (university of california, davis), and r. l. baldwin. enzymatic studies of pure cultures of rumen microorganisms. j. bacteriol. 92:1321-1330. 1966.-the activities of enzymes representing the major pathways of carbohydrate metabolism and anaerobic electron transport in cell-free extracts of whole rumen contents have been reported. the effects of diet upon the activities of several enzymes suggested that enzymatic measurements might prove useful for the study of rumen metabolism ...19664380801
characterization of several bovine rumen bacteria isolated with a xylan medium.dehority, b. a. (ohio agricultural research and development center, wooster). characterization of several bovine rumen bacteria isolated with a xylan medium. j. bacteriol. 91:1724-1729. 1966.-studies were conducted to characterize eight strains of bacteria isolated from bovine rumen contents, by use of a medium containing xylan as the only added carbohydrate source. based on morphology, biochemical reactions, nutritional requirements, and fermentation products, five of the eight strains were ide ...19665937235
[personal experiences in the treatment of closed abdominal trauma]. 19661264865
on the other side of the fence. 1966316113
biohydrogenation of unsaturated fatty acids. 3. purification and properties of a linoleate delta-12-cis, delta-11-trans-isomerase from butyrivibrio fibrisolvens. 19675633396
oxygen sensitivity of various anaerobic bacteria.anaerobes differ in their sensitivity to oxygen, as two patterns were recognizable in the organisms included in this study. strict anaerobes were species incapable of agar surface growth at po(2) levels greater than 0.5%. species that were found to be strict anaerobes were treponema macrodentium, treponema denticola, treponema oralis n. sp., clostridium haemolyticum, selenomonas ruminatium, butyrivibrio fibrisolvens, succinivibrio dextrinosolvens, and lachnospira multiparus. moderate anaerobes w ...19695370458
multiple antigenic components of the group-specific antigen of the avian leukosis-sarcoma viruses. 19694306591
pyrophosphate enhancement of hypocalcemic effect of thyrocalcitonin in rat. 19694309707
pyrophosphate enhancement of hypocalcemic effect of thyrocalcitonin in rat. 19694309707
a direct thiobarbituric acid-reacting chromogen in human red blood cells. 19704312812
characterization of the lipids of butyrivibrio fibrisolvens.butyrivibrio fibrisolvens strain d-1 was grown on a lipid-free chemically defined medium. the lipids were extracted with chloroform-methanol and separated into nonpolar and polar fractions by silicic acid column chromatography. further separations were made by preparative thin-layer chromatography. the lipid fractions were identified by specific staining reactions and r(f) values, by phosphorus and nitrogen determinations, by chromatography of hydrolysis products, and by the use of infrared spec ...19704316361
biohydrogenation of unsaturated fatty acids. iv. substrate specificity and inhibition of linoleate delta-12-cis, delta-11-trans-isomerase from butyrivibrio fibrisolvens. 19704990474
prolongation of canine renal homograft survival by post-transplantation treatment with antithymocyte serum. 19704392297
value of electrical pacing and beta adrenergic blockers in the prevention of fatal ventricular arrhythmias. 19714399445
effects of n-(2-diethylaminoethyl)-n-(2-hydroxy-2-phenylethyl)-2,5-dichloroaniline (an162) on platelet function and blood coagulation. 19715004226
the detection of microfilariae using the capillary haemotocrit tube method. 19715006086
experimental infection of beagle dogs with coxsackievirus type b1. 19715110117
experimental infection of beagle dogs with coxsackievirus type b1. 19715110117
carbon dioxide requirement of various species of rumen bacteria.the carbon dioxide requirement of 32 strains of rumen bacteria, representing 11 different species, was studied in detail. increasing concentrations of co(2) were added as nahco(3) to a specially prepared co(2)-free medium which was tubed and inoculated under nitrogen. prior depletion of co(2) in the inoculum was found to affect the level of requirement; however, the complexity and buffering capacity of the medium did not appear to be involved. an absolute requirement for co(2) was observed for e ...19715541030
biohydrogenation of unsaturated fatty acids. v. stereospecificity of proton addition and mechanism of action of linoleic acid delta 12-cis, delta 11-trans-isomerase from butyrivibrio fibrisolvens. 19715554292
[the replicative complexes of sendai virus]. 19714332921
biochemical changes in intestinal mucosa after experimental small bowel by-pass in the rat. 19724345813
the role of the tonsils in streptococcal infections: a comparison of tonsillectomized children and sibling controls. 19725010821
fermentation of isolated pectin and pectin from intact forages by pure cultures of rumen bacteria.studies on the rate and extent of galacturonic acid and isolated pectin digestion were carried out with nine strains of rumen bacteria (butyrivibrio fibrisolvens h10b and d16f, bacteroides ruminicola 23 and d31d, lachnospira multiparus d15d, peptostreptococcus sp. d43e, b. succinogenes a3c, ruminococcus flavefaciens b34b, and r. albus 7). only three strains, 23, d16f, and d31d, utilized galacturonic acid as a sole energy source, whereas all strains except a3c and h10b degraded (solubilized) and ...19724552890
recall of antibody synthesis to the primary antigen following successive immunization with heterologous albumins. a two-cell theory of the original antigenic sin. 19724563348
ftc-insulin conjugates. i. preparation of fluorescein thiocarbamyl-insulin conjugates. 19734579445
[various concepts and current observations on the neurohumoral control of prolactin secretion in mammals]. 19734585867
characterization of esterases produced by a ruminal bacterium identified as butyrivibrio fibrisolvens.an obligately anaerobic ruminal bacterial isolate was selected from 18 tributyrin-degrading isolates and identified as butyrivibrio fibrisolvens strain 53. the culture in late exponential phase contained enzymes which could be released by sonic disruption. these enzymes degraded substrates at a rate in the order 1-naphthyl acetate (na) > 1-naphthyl butyrate > 1-naphthyl propionate but did not degrade 1-naphthyl palmitate or 1-naphthyl phosphate. the enzymes on na were neither stimulated nor inhi ...19734734862
[changes in the rheographic tracing and in vascular resistance in the femoral circulatory region caused by intracarotid and intrafemoral injection of bradykinin]. 19734786556
enzymes of intermediary metabolism of butyrivibrio fibrisolvens and ruminococcus albus grown under glucose limitation. 19734796347
postweaning development of negative feedback in the pituitary-adrenal system of the rat. 19734353346
postweaning development of negative feedback in the pituitary-adrenal system of the rat. 19734353346
synaptic patterns in the visual cortex of the cat and monkey. electron microscopy of golgi preparations. 19734124647
[qualitative problems in analgesia by combined administration of beta-adrenolytic substances with local anesthetics]. 19734148793
cerebroside may be falsely identified as a soluble 'brain specific protein'. 19744213700
in vivo effect of lsd, morphine, ethanol and delta9-tetrahydrocannabinol on mouse brain adenosine triphosphatase activity. 19744272745
in vivo effect of lsd, morphine, ethanol and delta9-tetrahydrocannabinol on mouse brain adenosine triphosphatase activity. 19744272745
variations in enzyme activities of butyrivibrio fibrisolvens and ruminococcus albus grown in continuous culture. 19744275991
an antiproteolytic and antifibrinolytic preparation obtained from the potato by means of chromatography on se-sephadex c-50. 19744375312
emphysema in pathogen-free and bronchitic rats: an electronic lung-scanning technique. 19744811787
molecular species of ethanolamine phosphatides of dog and pig kidney. 19744425962
glycerol teichoic acid as an antigenic determinant in a gram-negative bacterium butyrivibrio fibrisolvens.an antigenic determinant isolated from a strain of the gram-negative bacterium butyrivibrio fibrisolvens reacted with specific antisera to the polyglycerophosphate backbone of membrane teichoic acids of lactobacilli. it gave a reaction of identity with membrane glycerol lipoteichoic acid and glycerol teichoic acid preparations from lactobacilli, and with phenol extracts of other gram-positive bacteria. the antigen-antibody reactions was strongly inhibited by glycerol-phosphoryl-glycerol-phosphor ...197550403
purification by affinity chromatography, properties and crystallisation of phosphofructokinase from thermophilic micro-organisms. 1975124670
hydrophobic interactions of the apo-gln-i polypeptide component of human high density serum lipoprotein.apo-gln-i, the major polypeptide component of human serum high density lipoprotein, has four noninteracting hydrophobic sites which associate with alkanes, anionic detergents, and cationic detergents. hexane and octane bind to these sites with association constants of 6.8 times 10-2 and 1.8 times 10-4 liters/mol, respectively, and compete with the anionic detergent, sodium dodecyl sulfate (c12oso3-minus), at low detergent ligand binding ratios (i.e. smaller than or equal to 1.0 mol of c12oso3-mi ...1975165177
reversible hypothyroidism in growth hormone-deficient children treated with human growth hormone.six children with human growth hormone (hgh) deficiency became hypothyroid during the course of their therapy with hgh. this was accompanied by a decreasing growth rate, clinical symptoms of hypothyroidism and decreased serum t4 concentrations. three of the 6 patients returned to the euthyroid state, both clinically and biochemically, with cessation of hgh therapy, and reinstitution of hgh precipitated hypothyroidism again in 2 of the three. the patients who remained hypothyroid have evidence of ...1975165211
structural and functional characteristics of the microcirculation in neoplasms.the microcirculation in a transplantable rat fibrosarcoma was investigated. the vasculature of the neoplasm consisted of irregular channels lined by plump endothelium which displayed mainly pentalaminar although a few heptalaminar junctions were also found. few pericytes surround the endothelial layer while the basement-membrane varied in thickness and was often duplicated and triplicated. challenge with histamine resulted in increased permeability in comparison with normal connective tissues si ...1975168332
studies on the free radicals in myocardial mitochondria by electron spin resonance (est) spectrometry (studies on experimental infarction dogs). 1975168418
superfusion techniques for assessment of steriod hormone production in endocrine tissue and isolated cells cadrenal. 1975168465
conditional lethal mutants of vesicular stomatitis virus. 1975169102
binding of digitoxin to human serum proteins: influence of ph on the binding of digitoxin to human albumin. 1975238355
protamine kinase from yeast.a protein kinase (atp: protein phosphotransferase, ec 2.7.1.37) which preferentially phosphorylates protamine is purified about 250-fold from the soluble fraction of baker's yeast (saccharomyces cerevisiae). this enzyme is not sensitive to activation by cyclic nucleotides. histone is about 5% as active as protamine in the reaction rate. neither casein, phosvitin nor glycogen phosphorylase is active as substrate. the enzyme is distinguishable from casein kinase of the classical type (rabinowitz, ...1975238628
comparison of the cardiostimulatory effects of nicotine in dogs and monkeys. 1975241822
in vitro production of erythropoietin by mouse fetal liver.mouse fetal liver tissue has been cultured and shown to produce and release into the culture medium an erythropoietically active substance for up to 30 days of culture. since this substance can be completely neutralized by an antiserum to erythropoietin and shows a dose--response relationship in the plethoric mouse assay, it is suggested that the culture medium contains erythropoietin, a hormone important in the regulation of erythropoiesis. using this procedure, we have obtained the equivalent ...19751131429
[periodicity of changes in halobacterium cultures]. 19751183309
the isomerization of 2,5- and 9,12-octadecadienoic acids by an extract of butyrivibrio fibrisolvens.a cell-free particulate preparation from butyrivibrio fibrisolvens was used to study the relative rates of isomerization of all cis,cis-methylene-interrupted isomers of octadecadienoic acid. only two isomers were found to be substrates, the 9,12-isomer was isomerized at 41 +/- 4 mumol/min per mg protein, and the 2,5-isomer at 11 +/- 1 mumol/min per mg. the product of the isomerization of the 2,5-isomer had an ultraviolet absorption maximum at 233 nm indicating that it was the 3,5-isomer. the iso ...19761252494
biohydrogenation of unsaturated fatty acids. hydrogenation by cell-free preparations of butyrivibrio fibrisolvens.hydrogenation of cis-9,trans-11-octadecadienoic acid to yield trans-11-octadecenoic acid by cell-free preparations of butyrivibrio fibrisolvens has been obtained under strictly anaerobic conditions. reduced methyl viologen, nadh, and an endogenous electron donor each can serve as a reductant. inhibition studies and gel filtration patterns reveal the presence of at least two hydrogenation systems, one of which is coupled through a flavin, possibly fmn. although the enzymes comprising the biohydro ...19761262324
isolation of lipoteichoic acids from butyrivibrio fibrisolvens.lipoteichoic acid (lta) and deacylated lipoteichoic acid have been isolated from the bovine-rumen gram-negative anaerobe butyrivibrio fibrisolvens by phenol extraction. lipoteichoic acid (21.8 mumol phosphorus/g cells) consisted of a conventional 1, 3-phosphodiester-linked chain of glycerol phosphate units joined covalently to a glycolipid. it was not substituted with glycosyl or d-alalyl ester groups. deacylated lipoteichoic acid (57.5 mumol phosphorus/g cells) was similar in constitution but l ...1976819620
agonist and antimineralocorticoid activities of spirolactones.to investigate the mechanism of action of antimineralocorticoids, a series of spirolactone analogues was evaluated for both mineralocorticoid antagonist and agonist activity. antagonist activity was assessed by inhibition of aldosterone stimulated sodium transport employing toad bladder short-circuit current (scc) measurements. agonist activity was assessed in the same system by the direct effect of spirolactones on scc. opening of the gamma-lactone ring of a spirolactone dramatically decreased ...1976183514
equine herpesviruses. 6. sequential infection of horses with types 2, 3 and 1.the immunological and virological status of 3 foals in respect of equine herpesviruses (ehv) was established and the foals were sequentially infected with ehv2, ehv3 and ehv1. following experimental infection with ehv2, no clinical signs of disease were observed in any foal. the inoculation of ehv3 into the genital tract resulted in lesions of the mucous membrane and perineal skin that were considered typical of equine coital exanthema. following intransal inoculation of ehv3 extensive ulceratio ...1976183644
duck viral enteritis: a comparison of replication by ccl-141 and primary cultures of duck embryo fibroblasts.cultures of primary cells and a line of fibroblast-like cells from the pekin duck were both compared for their replication of the herpesvirus of duck viral enteritis. the two kinds of cells were equally accurate for quantifying virus upon isolation. also, one-step growth curves showed that in both kinds of cultures new virus appeared by the 18th hour and that infectivity peaked at about 36 hours. primary cultures yielded about 5.6 times as much virus as did the cell line, though plaques were mor ...1976183645
the effects of prostaglandins e1 and e2 on heart rate responses to cervical sympathetic nerve stimulation and gaglion stimulant drugs in the anaesthetized mouse [proceedings]. 1976184870
cultural factors influencing the utilization or production of acetate by butyrivibrio fibrisolvens.utilization of acetate by four strains of butyrivibrio fibrisolvens was influenced by the composition of their growth medium. growth-limiting glucose concentrations, low availability of co2 and the presence of sodium lactate all stimulated acetate uptake by three strains. the type strain, d1, utilized acetate if the concentration of acetate added to the medium was at least 15 mumol ml-1. in batch culture, all strains produced acetate before entering a phase of acetate uptake. continuous-culture ...1976985851
some aspects of the metabolism of butyrivibrio fibrisolvens.the growth responses of an acetate-utilizing isolate of butyrivibrio fibrisolvens to co2, acetate and pyruvate were determined using a chemically-defined medium. carbon dioxide was essential for growth and both acetate and pyruvate increased growth. 14c from [i-14c]pyruvate appeared predominantly in formate and lactate. these results, together with those obtained with enzyme preparations, indicated pyruvate synthase, pyruvate-co2 exchange and pyruvate formate lyase to be active.1976993781
ampicillin-resistant hemophilus sp. 19761082793
simian virus 40 facilitates multiplication of replication defective mutants of polyoma virus in balb/3t3 mouse cells. 1977195219
proposed effects of brain noradrenaline on neuronal activity and cerebral blood flow during rem sleep.we propose that the observed increases of both neuronal activity and cerebral blood flow seen throughout the brain during rem sleep may be effects of decreased central noradrenaline release.1977201486
fibrous histiocytoma of the tarsus.a 21-year-old white man had an asymptomatic eyelid mass that had slowly enlarged for one year. on clinical examination the lesion was well circumscribed; it appeared subconjunctivally but provoked no conjunctival inflammation, and had a consistency suggestive of cartilage. microscopic examination showed it was matted and composed of elongated fibro-histiocytes and an evenly distributed population of xanthoma cells and touton giant cells. the lesion was easily removed by local excision because of ...1977202162
studies of membrane fusion. ii. fusion of human erythrocytes by sendai virus. 1977202607
ultrastructure of butyrivibrio fibrisolvens: a gram-positive bacterium.the cells of bacteria of the genus butyrivibrio are universally described as being gram negative, and they produce an unequivocal gram-negative reaction in the standard staining procedure. however, their cell walls contain derivatives of teichoic acid, which are characteristic of gram-positive cells. in this study, the cell walls of two representative strains of butyrivibrio were of the gram-positive morphological type, as seen by electron microscopy, but they were very thin (12 to 18 nm). the t ...1977845122
activation of nad-dependent lactate dehydrogenase in butyrivibrio fibrisolvens by fructose 1,6-diphosphate. 1977874457
characterization of the predominant bacteria occurring in the rumen of goats (capra hircus).a total of 44 strains of bacteria were isolated from rumen contents of the goat. based on morphology, gram stain, anaerobiosis, motility, and fermentation end products, they were grouped into 11 different types. for each type, all or representative strains were characterized in detail. the type, number of strains characterized over total number of strains, and identification were as follows: type 1, 6/21, atypical butyrivibrio fibriosolvens; type 2, 6/9, atypical butyrivibrio fibrisolvens; type ...1977879766
studies on some characteristics of hydrogen production by cell-free extracts of rumen anaerobic bacteria.hydrogen production was studied in the following rumen anaerobes: bacteroides clostridiiformis, butyrivibrio fibrisolvens, enbacterium limosum, fusobacterium necrophorum, megasphaera elsdenii, ruminococcus albus, and ruminococcus flavefaciens. clostridium pasteurianum and escherichia coli were included for comparative purposes. hydrogen production from dithionite, dithionite-reduced methyl viologen, pyruvate, and formate was determined. all species tested produced hydrogen from dithionite-reduce ...1977558042
dissociation of cyclic gmp from the negative inotropic action of carbachol in guinea pig atria.the relationship between the negative inotropic action of carbachol and its ability to elevate cyclic gmp was determined in isolated paced guinea pig atria. a clear dissociation was observed between that concentration of carbachol which depressed contractility and that which elevated tissue cyclic gmp content. doses as low as 0.03 micronm caused a negative inotropic effect while cyclic gmp was not elevated until concentrations nearly 100-fold higher were used. thus a correlation between tissue c ...1977203613
locust oviposition: stress softening of the extensible intersegmental membranes. 1977235129
[sinusitis]. 1978242945
electron microscopy of the microbial populations present and their modes of attack on various cellulosic substrates undergoing digestion in the sheep rumen.cotton fibers and various cell wall preparations from grass leaves and from the feces of sheep fed on dried grass were placed in the sheep rumen in bags made from 5-mum-mesh nylon cloth. after periods of from 3 to 48 h, bags were removed, and the contents were fixed, embedded, sectioned, and stained for electron microscopy. some of the bacteria present were seen to be closely associated with the cell walls, either tunneling within them or making very close contact. evidence was obtained for diff ...197816345303
mammary tumors, hepatocellular carcinomas, and pancreatic islet changes in c3h-avy mice.studies of tumor incidence and assorted lesions found in 187 c3h-avy mice throughout their natural life-spans revealed the following: hepatocellular carcinomas occurred in 54.3% of males, mammary carcinomas in 95% of females, pancreatic islet cell adenomas in 9.4% of males and in no females, and pancreatic islet cell hyperplasia in 41% of males and 23% of fefemales. islet cell hyperplasia and adenomas appeared to consist predominantly of alpha and delta cells. multiple tumors, or hyperplasia, or ...1978203711
human lymphoblastoid cells as hosts for parvoviruses h-1 and rat virus.a human t-cell line (molt-4) was shown by viral hemagglutination and infectivity assays to support the replication of rat virus (rv) and h-1 virus. in addition, h-1 virus, but not rv, multiplied in two human b-cell lines, av-1 and nc-37. the ability to bind radioactively labeled rv was demonstrated for each of the cell lines, but viral adsorption occurred to a greater degree with molt-4 cells than with either av-1 or nc-37 cells. after challenge with rv, virus-specific antigens were detected in ...1978203718
necrotizing pancreatitis due to simian adenovirus type 31 in a rhesus monkey.simian adenovirus type 31 was isolated from pancreatic tissue of an 8-year-old male rhesus monkey that died as the result of acute necrotizing pancreatitis. histologically, the pancreas showed wide-spread necrosis, extensive infiltration of polymorphonuclear leukocytes, and nuclear inclusions in pancreatic acinar cells. large numbers of adenovirus particles were demonstrable in the acinar cells by electron microscopy. this is the second case of adenovirus-associated pancreatitis we have found in ...1978205187
the "sarcoma-specific" region of moloney murine sarcoma virus 124.labeled, purified 30s rna from moloney murine sarcoma virus was annealed to an excess of moloney murine leukemia virus complementary dna. upon treatment of the resulting dna.rna hybrids with rnase h followed by sucrose gradient sedimentation, and undigested 18s rna molecule was recovered. this rna molecule was shown to represent the "sarcoma-specific" region of the virus. the unintegrated linear dna provirus of murine sarcoma virus 124 was isolated from newly infected cells and a physical map of ...1978208071
effects of heavy metals and other trace elements on the fermentative activity of the rumen microflora and growth of functionally important rumen bacteria.the inhibitory effects of high concentrations of essential and non-essential trace elements were tested on the rumen microflora using the rate of fermentation in vitro as the assay. the elements (and the concentration causing 50% inhibition) in decreasing order of toxicity were hg2+ (20 microgram/ml), cu2+ (21 microgram/ml), cr6+ (70 microgram/ml), se4+ (73 microgram/ml), ni2+ (160 microgram/ml), cd2+ (175 microgram/ml), as3+ (304 microgram/ml) and as5+ (1610 microgram/ml). the elements tested t ...1978565671
production of branched-chain volatile fatty acids by certain anaerobic bacteria.net production of isobutyric acid, isovaleric acid, and 2-methylbutyric acid by cultures of bacteroides ruminicola and megasphaera elsdenii on media that contained trypticase or casein hydrolysate continued (up to 5 days) after growth had ceased. only trace quantities of these acids were produced in a medium that contained a mixture of amino acids that did not include the branched-chain amino acids. m. elsdenii produced increased quantities of the branched-chain fatty acids in a medium that cont ...1978566082
the role of carbonate in the metabolism of glucose by butyrivibrio fibrisolvens.the amount of na2co3 added to semi-synthetic medium determined the length of the lag phase, the growth rate and the dry weight of three strains of butyrivibrio fibrisolvens (wv1, nor37, b835). with increasing co3(2-) concentration the molar growth yield of bacteria, from glucosewas increased and, of the fermentation products, formate increased more than the other acids. co3(2-)-limited cultures of strain wv1 (group 2 butyrivibrio) and strain nor37 (troup 1 butyrivibrio) incorporated 14co3(2-) in ...1978641526
a universal lipid exchange protein from rat hepatoma.the postmicrosomal protein fraction from rat hepatoma 27 adjusted to ph 5.1 stimulates phospholipid exchange between rat liver microsomes and mitochondria with higher rates and in a less specific way than the corresponding fraction from rat liver. a phospholipid exchange protein has been purified to homogeneity from the hepatoma ph-5.1 supernatant by gel filtration on sephadex g-75 and ion-exchange chromatography on carboxymethylcellulose. the isolated protein had a molecular weight of 11200 as ...1978203454
an unstable donor-recipient dna complex in transformation of bacillus subtilis.in re-extracted dna obtained shortly after uptake of transforming dna by bacillus subtilis, increased amounts of donor dna radioactivity banding at the position of donor-recipient dna complex (drc) are observed in cscl gradients, if the cells are irradiated with high doses of uv prior to reextraction of the dna. qualitatively, the same phenomenon is observed if lysates of transforming cells are irradiated. uv-irradiation of lysates of competent cells to which single-stranded dna is added after l ...1978106232
[vascularization of the hypothalamic-hypophyseal area of the toad bufo bufo (l.). light and scanning electron microscopy studies]. 1978106569
ultrastructure and development of an exosporium-like outer spore envelope in bacillus subtilis.an exosporium-like outermost envelope is occasionally observed in thin sections of bacillus subtilis spores. treatment of the mature spores with urea and mercaptoethanol (sometimes completed by sodium dodecyl sulfate) or with naoh, disorganizes and partially solubilizes the outer spore coat. this treatment permits a clear visualization of the exosporium in all spores of several b. subtilis strains. exosporium appears either as a single sheet, 8-9 nm thick, or with a triple-layered unit membrane- ...1978106753
inhibitory effect of hypothalamic lesions on liver tumor induction by n-2-fluorenylacetamide in male rats.bilateral electrolytic lesions were placed in the median eminence area of the hypothalamus in 12-week-old male wistar rats. sham-operated and untreated control rats were also included. two weeks later, one-half of them were given 0.03% n-2-fluorenylacetamide incorporated into the diet for 16 weeks with adequate resting periods in between. the animals were killed 34 weeks after the last carcinogen feeding. the results show that lesions in the hypothalamus effectively inhibited liver tumor formati ...1978201376
demonstration of 5'-methylthioadenosine phosphorylase activity in various rat tissues. some properties of the enzyme from rat lung.an enzyme (5'-methylthioadenosine phosphorylase) that catalyzes the phosphorolytic cleavage of 5'-methylthioadenosine to 5-methylthioadenosine to 5-methylthioribose-1-phosphate and adenine was found in various rat tissues. liver and lung had the highest enzyme activities and heart the lowest, most of the activity (greater than 90%) was recovered in soluble tissue fractions. the enzyme from rat lung was purified about 30-fold by ph treatment (nh4)2so4 fractionation, and gel filtration. the enzyme ...1978415762
biohydrogenation of unsaturated fatty acids. presence of dithionite and an endogenous electron donor in butyrivibrio fibrisolvens.two oxygen-consuming substances were isolated from cell-free extracts of the rumen anaerobe, butyrivibrio fibrisolvens. the major fraction comprising 97% of the total activity was characterized as a three-component mixture of glucose, maltose, and dithionite. the minor activity fraction contained an electron donor for the reduction of cis-9,trans-11-octadecadienoate to trans-11-octadecenoate. after oxidation, the electron donor could be reduced by the dithionite, thereby accounting for the previ ...1979438161
some effects of uncouplers and inhibitors on growth and electron transport in rumen bacteria.uncouplers and inhibitors of electron transport affected growth and electron transport of rumen bacteria in various ways. selenomonas ruminantium was not affected by inhibitor and uncoupler concentrations which affected growth and electron transport of bacteroides ruminicola, b. succinogenes, and butyrivibrio fibrisolvens. inhibitors, when active, led to accumulation of reduced electron carriers before the site of action, but differences were found among organisms in the site of action of these ...1979457609
bacterial and strain specificities in opsonization, phagocytosis and killing of streptococcus mutans.opsonization of streptococcus mutans, followed by phagocytosis and killing by polymorphonuclear leucocytes has been postulated as an effector mechanism in protection against dental caries. opsonization was studied by using sera from monkeys immunized with killed strep. mutans (sero-type c) and compared with sera from sham-immunized monkeys. antibodies to strep. mutans (sero-type c) induced maximal phagocytosis and killing of serotypes c and e, and this was significantly greater than with serotyp ...1979106996
experimental and postexperimental response to anteriorly directed extraoral force in young macaca nemestrina.an anteriorly directed extraoral force was applied to the maxillas of four healthy young m. nemestrina monkeys. experimental, retention, and postretention alterations were evaluated through a combination of cephalometric, histologic, and gross techniques. in view of the results of the present study, the following conclusions can be made: 1. skeletal remodeling occurs in all circummaxillary sutures following the application of an anteriorly directed extraoral force to the maxilla. the amount of r ...1979107808
creutzfeld-jakob disease and corneal grafts.the possible danger of surgical transmission of creutzfeld-jakob disease from one human being to another has been reported in three instances. although laboratory attempts at transmission of this disease by corneal grafting have so far failed in animals, it is emphasized that great care should be exercised in the selection of donor material for corneal grafts and renal transplants.1979111011
[radioautographic identification of dopaminergic axons in the rat cns. in vitro marking with tritiated dopamine or noradenaline].a new radioautographic technique for the visualization of central dopaminergic fibers is described. this technique is based upon in vitro uptake of triatiated norepinephrine or tritiated dopamine in the presence of monoamine oxidase inhibitor and specific uptake inhibitor in noradrenergic fibers. after a short step in glutaraldehyde, the use of osmium vapours as a fixative, instead of osmic acid immersion, allows a satisfactory retention of triatiated amines within dopaminergic fibers.1979120791
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