Publications

[muscarinic receptors of the superior cervical lymph node of the cat].		1966	4386667
significance of achromobacter nematophilus poinar and thomas (achromobacteraceae: eubacteriales) in the development of the nematode, dd-136 (neoaplectana sp. steinernematidae).		1966	4960247
hidden antigenic determinant in secretory immunoglobulin a.		1968	4176458
effects of steroid and nonsteroid metabolites on enzyme conformation and pyridoxal phosphate binding.		1969	4391266
sensitivity of klebsiella, enterobacter, and escherichia coli to cephalothin.		1969	4885058
a hydrogen-bond network at the active site of subtilisin bpn'.		1970	4399039
[development of polyribosomes during maturation of reticulocytes in vivo].		1970	4098024
[stabilization of n-antigens of enteroviruses].		1970	4322838
dendroctonus frontalis infection by the dd-136 strain of neoaplectana carpocapsae and its bacterial complex.	the dd-136 strain of neoaplectana carpocapsae weiser (steinernematidae) after spray application to pine bark in 0.1% formalin plus wetting agent entered pine bark beetle tunnels and killed 44% of the brood and adults of dendroctonus frontalis zimmermann at 18 and 26 c, 60% relative hummidity and at ambient temperatures and humidities.	1970	19322322
isolation of heart-resistant clostridium perfringens from healthy cattle.		1972	4333445
[electrophysiologic, histochemical and immunologic correlates in the central mechanism of audiogenic epilepsy].		1972	4336346
[response of the recipient's tissue bed and features of reparative osteogenesis in retransplantation of bone tissue].		1972	4553209
biochemical aspects of some pig muscle disorders.		1972	4553607
phases of the inflammatory response to subcutaneous implantation of a cotton pellet and their modification by certain anti-inflammatory agents.		1972	4562620
ultrastructure of achromobacter nematophilus poinar et thomas bacteria.		1972	5052048
relationship of rumen volatile fatty acids to type of grain,sorghum grain processing method and feedlot performance.		1972	5055758
"uterine" and "luteal" effects of prostaglandins (pg) in rats and guinea pigs as potential abortifacient mechanisms.		1973	4542124
cyclizing compounds. 3. local anesthetic action of n-(omega-haloalkyl)-n-methylaminoaceto-2,6-xylidides.		1973	4542166
antibody-dependent lymphocyte-mediated cytotoxicity in man: no requirement for lymphocytes with membrane-bound immunoglobulin.		1973	4542262
bilateral tubal pregnancy with an unusual complication.		1973	4570658
the mechanism of action of beta-galactosidase. effect of aglycone nature and -deuterium substitution on the hydrolysis of aryl galactosides.	1. steady-state kinetic parameters for the beta-galactosidase-catalysed hydrolysis of 13 aryl beta-d-galactopyranosides show no simple dependence on aglycone acidity. 2. alpha-deuterium kinetic isotope effects (k(h)/k(d)) for seven of these substrates, measured under steady-state conditions with [s]>>k(m), vary from 1.00 for poor substrates to 1.25 for hydrolysis of the galactosyl-enzyme. 3. methanolysis of the galactosyl-enzyme in 1.5m-methanol increases k(h)/k(d) for degalactosylation, but lea ...	1973	4578762
biogenesis of mitochondria. xxv. studies on the mitochondrial genomes of petite mutants of yeast using ethidium bromide as a probe.		1973	4583801
effects of photooxidation of histidine-38 on the acetylphosphatase activity of glyceraldehyde-3-phosphate dehydrogenase.		1973	4345586
suppression of herpes simplex virus infection by phosphonoacetic acid.	disodium phosphonoacetate when administered orally or topically to mice experimentally infected with herpes simplex virus was able to significantly reduce the mortality associated with the agent. in addition, this compound was able to reduce herpesvirus lesions on the corneas of infected rabbits.	1973	4356456
the vaccination of man and other animals against rabies.		1973	4207636
studies on the therapeutic use of mercurascan (msc). part 2: antiarrhythmogenic and haemodynamic effect of msc in experimental myocardial ischaemia.		1974	4140846
analgesia produced by clonidine in mice and rats.		1974	4152777
effect of prostaglandin f2alpha on the luteal phase of the cycle in nonpregnant women.	11 healthy nonpregnant female volunteers were treated with (pgf) prostaglandin f2alpha as the tham salt in aqueous solution administratered intravaginally. (hcg) human chorionic gonadotropins was also administered to 9 of these women prior to or following the pg therapy to simulate secreting the phoblast. it was considered that luteolysis occurred if serum progestin declined to preovulatory levels, if there was no increase of serum progestin levels following hcg, and if there was shortening of ...	1974	4429112
[specificity of antibodies in experimental trichinosis of the rat].		1974	4455270
[fever, edema, eosinophilia and acute pneumonia: bilateral, diffuse pneumocystis carinii pneumonia and candida pneumonia of the left lung (evaluation of eosinophilia)].		1975	1082519
[effectiveness of fibrinolytic agents in complex treatment of traumatic endophthalmitis].		1975	1082561
proceedings: effects of histamine, 2-methylhistamine and 4-methylhistamine on blood pressure and vascular resistance in the cat.		1975	1151865
effect of heat-killed cells of achromobacter nematophilus poinar et thomas, and the fraction (endotoxin) isolated from them on galleria mellonella l. caterpillars.		1975	1192248
[effusions in the dog. 1. clinico-diagnostic study with special reference to the cytology of the fluid].		1975	1231052
the influence of sodium, potassium and lanthanum on amino acid release from spinal-medullary synaptosomes.		1975	166144
aryl hydrocarbon hydroxylase induction in mammalian liver-derived cell cultures. effects of various metabolic inhibitors on the enzyme activity in hepatoma cells.		1976	181006
surveillance of pertussis in the cssr. iv. immunological surveys of antibodies to pertussis and parapertussis in the bohemian regions and in slovakia in 1958 - 1971.		1976	184206
the lecithin: cholesterol acyltransferase reaction, lysolecithin and red cell ageing in blood stored under normal transfusion service conditions.	the lecithin:cholesterol acyltransferase (lcat) activity and lysolecithin content of human blood stored under standard blood transfusion service conditions at 4 degrees c for 6 weeks has been investigated. cooling the blood to 4 degrees c rapidly inactivates the lcat reaction, but the enzyme is not denatured during storage under these conditions. citrate in the anticoagulant did not activate the lcat reaction in freshly-taken whole blood. the total phospholipid and total lysolecithin content of ...	1976	1251568
correspondence: growth plate defects in foals.		1976	1265980
changes in the plasma lipoprotein-apoproteins of guinea pigs in response to dietary cholesterol.	the major apoproteins from four plasma lipoproteins were isolated from control and cholesterol-fed guinea pigs. apoproteins were studied by column chromatography, polyacrylamide gel electrophoresis, and amino acid analysis. dietary cholesterol altered the plasma apolipoproteins mainly by an enrichment in the content of arginine-rich polypeptide (arp) in all density fractions. this protein had a similar molecular weight (34 000), electrophoretic mobility, amino acid composition, and microheteroge ...	1977	201278
juvenile periodontitis (periodontosis).	juvenile periodontitis, characterized by early age of onset, rapid rate of periodontal tissue destruction and selective tooth involvement in the absence of commensurate aetiological factors, is recognized as a distinct clinical entity. the present paper describes the clinical findings and some immunological characteristics in a group of thirty-four patients under the age of thirty. clinical aspects studied included the influence of sex, race, family history and general health. immunological find ...	1977	267621
location of adult and fetal aldolases a, b, and c by immunoperoxidase technique in lf fast-growing rat hepatomas.	the resurgence of aldolase isozymes in cancerous tissues is a well-known but poorly understood phenomenon. this resurgence poses the problem of whether or not adult and fetal aldolase isozymes are produced by the same cells. for clarification of this question, the immunoperoxidase technique was used to locate aldolases a, b, and c in one type of fast-growing hepatoma, the lf hepatoma and, by comparison, in normal adult liver. under optical microscopy, aldolases a and c were located in the cytopl ...	1978	201371
ultrastructural observations on the maturation and secretion of granules in atrial myocardium.		1978	99521
behavioral measurement measurements of excitability changes in reward sites in the dog: a frequency dependent effect.		1978	99757
repair of deaminated cytosine residues of dna: biological significance of the absence of uracil from dna.		1978	100113
evaluation of the role of c4 in the cellular immune response in vitro.		1979	156225
histochemistry of tissue reaction in the heart of cattle experimentally infected with cysticercus bovis.		1979	161543
ia determinants on human t-cell subsets defined by monoclonal antibody. activation stimuli required for expression.	the nature of ia antigens which appear on human t cells after activation and the stimuli required for their expression was examined utilizing a monoclonal antibody reactive with the ia antigen framework. t cells were purified using monoclonal antibodies directed either at the entire t-cell population (okt3) or the t-cell inducer subset (okt4). by indirect immunofluorescence, it was shown that the human t-cell population contains no detectable ia+ cells in the resting state. in contrast, in exces ...	1979	92523
[physicochemical properties of neurospecific basic protein (scp) of the spinal cord].	basic protein (scp) known as antiencephalytogenic was isolated from the bull spinal cord and spinal roots. the protein is purified to a molecular homogeneous state, some of its physicochemical properties are studied. molecular homogeneity of the protein is established by the method of disk electrophoresis in 15% polyacrylamide gel with sodium dodecyl sulphate present and by analytic ultracentrifugation. during electrophoresis in 15% polyacrylamide gel at ph 4.0 the protein movement occurs only i ...	1979	92837
penicillin and cephalosporin immunogenicity in man.	whether cross-allergenicity exists between the penicillin and cephalosporin antibiotics is controversial. a solid-phase radioimmunoassay was adapted to detect drug-specific igg, igm and ige antibodies. penicillin and cephalothin-specific serum immunoglobulins were determined in patients' serum before and after a therapeutic course of sodium cephalothin. changes in igg, igm and ige antipenicillin and anticephalothin antibodies, respectively, occurred and were closely correlated to one another (r ...	1979	92902
from the nichd. national institute of child health and human development. predictors of hereditary disease or congenital defects.		1979	93264
anomalous alpha 2-macroglobulin-protease complexes in cystic fibrosis: decreased uptake of the complexes by fibroblasts in culture.	immunochemical and functional properties of control and cystic fibrosis (cf) alpha 2-macroglobulin (alpha 2m) are compared. crossed immunoelectrophoresis and ouchterlony double diffusion revealed no qualitative differences between the two alpha 2-m preparations. trypsin-esterase activity assayed with bapna as a substrate, in the presence of an excess sti, gave similar ratios between total and active alpha 2m. these alpha 2m-trypsin complexes were equally stable under various experimental conditi ...	1979	93265
[histochemical studies on the structure and the age changes of descemet's membrane in cattle (author's transl)].	descemet's membrane of 15 cattle-corneas, aged 6 month to 13 years was studied by various histochemical and morphometric methods. 3 zones could be differentiated. zone 1 is relatively small and does not show significant changes with age; zone 2 comprises the main part of descemet's membrane, its diameter increased with age. zone 2 is rich in sulfated glycosaminoglycans. zone 3, below the endothelial lining, does not develop before the 3rd and 5th year of life and stains different from zone 2.	1979	94246
cytogenetic analysis of double satellites on marker chromosome no. 14.		1979	95131
effect of glucose and insulin on protein and basement membrane synthesis in isolated glomeruli of diabetic rats.	in incubation experiments with isolated glomeruli, an increased synthesis of protein and basement membranes was detected in diabetic rats compared to metabolically healthy controls. different glucose concentrations in the incubation medium and insulin did not influence protein and basement membrane synthesis of nondiabetic glomeruli. on the other hand, in diabetic glomeruli the synthetic activity depends on glucose concentration. insulin had a stimulating effect on protein and basement membrane ...	1979	525208
effect of the entomogenous nematode nemplectana carpocapsae on the tachinid parasite compsilura concinnata (diptera: tachinidae).	the entomogenous nematode neoaplectana carpocapsae and its associated bacterium, xenorhabdus nematophilus, could not infect the pupal stage of the tachinid compsilura concinnata through the puparium. n. carpocapsae had an adverse effect on 1-, 2- and 3-day-old c. concinnata larvae within the armyworm host in petri dish tests. all 1-day-old larvae treated with nematodes died in their hosts, whereas 61% and 69% of 2- and 3-day-old larvae treated with nematodes, respectively, died. however, the sur ...	1984	19295866
growth and virulence of steinernema glaseri influenced by different subspecies of xenorhabdus nematophilus.	three xenorhabdus nematophilus subspecies influenced steinernema glaseri growth profiles and growth rates, but this was not necessarily because of different bacterial growth rates. virulence of dauer nematodes in larval galleria mellonella varied with the number of dauers retaining bacteria and the bacterial subspecies. virulence was least for dauers grown on x. nematophilus subsp. bovienii because of the lack of retained bacteria. virulence was subsequently restored by culturing these nematodes ...	1985	19294127
expression of the gene for bacillus subtilis aspartokinase ii in escherichia coli.	the gene coding for the subunits of aspartokinase ii from bacillus subtilis has been identified in a b. subtilis dna library and cloned in a bacterial plasmid (bondaryk, r. p., and paulus, h. (1984) j. biol. chem. 259, 585-591). the introduction of a plasmid carrying the aspartokinase ii gene into an auxotrophic escherichia coli strain lacking all three aspartokinases restored its ability to grow in the absence of l-lysine, l-threonine, and l-methionine. the b. subtilis aspartokinase gene could ...	1985	2981222
[comparative study of lipoteichoic acid from streptococcus pyogenes type 29 isolated by different methods].	a comparative study of lipoteichoic acid preparations extracted from streptococcus pyogenes with cold and hot phenol and trichloracetic acid was made. the most delicate way for isolation of lipoteichoic acid from the given microorganisms is cold phenol extraction.	1985	3911202
the oxygen tension and temperature of the superior palpebral conjunctiva.	a clark-type oxygen sensor and temperature-sensing thermistor embedded in a ring designed to fit into the human palpebral aperture were used to directly measure the oxygen tension and temperature of the upper palpebral conjunctiva in 16 human subjects. the mean oxygen tension was found to be 61.4 +/- 6.9 mmhg, equivalent to an atmosphere of 8.2% oxygen, slightly higher than previously reported. the mean temperature was 36.2 +/- 0.6 degrees c, in good agreement with previous findings. significant ...	1985	3993337
diurnal rhythms in calcium and phosphate metabolism in rodents and their relations to lighting and feeding schedules.	diurnal fluctuations in serum ca, inorganic phosphorus, mg, and alkaline phosphatase activity and their relations to lighting and feeding schedules were investigated in nocturnal rats and diurnal asiatic chipmunks. the following results were obtained: all parameters in the animals fed ad libitum and adapted to a regulated light-dark cycle (with light from 7.00 to 19.00 h) showed diurnal rhythms; under the same experimental condition, the patterns of the rhythms in the nocturnal rat and the diurn ...	1985	4040208
[immunological study of anti-cancer effects of human alpha interferon for advanced renal cell carcinoma].		1985	4079199
nadph/nadh-dependent cold-labile glutamate dehydrogenase in azospirillum brasilense. purification and properties.	a cold-labile glutamate dehydrogenase (gdh, ec 1.4.1.3) has been purified to homogeneity from the crude extracts of azospirillum brasilense. the purified enzyme shows a dual coenzyme specificity, and both the nadph and nadh-dependent activities are equally cold-sensitive. the enzyme is highly specific for the substrates 2-oxoglutarate and glutamate. kinetic studies with gdh indicate that the enzyme is primarily designed to catalyse the reductive amination of 2-oxoglutarate. the nadp+-linked acti ...	1986	3956501
a new sialoglycoprotein from rat seminal vesicle and its association with semen coagulum.	by radiolabeling using naio4/[3h]kbh4, a new sialoglycoprotein with mr of 19,000 was found in the secretion of rat seminal vesicle. it was shown to interact non-covalently with semen coagulum. it existed in three acidic forms with pi values of 4.1, 3.7 and 2.9 and possessed high contents of sialic acids and acidic amino acids.	1986	3707590
mechanism-based inhibition of lactoperoxidase by thiocarbamide goitrogens.	the irreversible inactivation of bovine lactoperoxidase by thiocarbamide goitrogens was measured, and the kinetics were consistent with a mechanism-based (suicide) mode. sulfide ion inactivated, 2-mercaptobenzimidazole-inactivated, and 1-methyl-2-mercaptoimidazole-inactivated lactoperoxidases have different visible spectra, suggesting different products were formed. the results support a mechanism in which reactive intermediates are formed by s-oxygenation reactions catalyzed by lactoperoxidase ...	1986	3768307
scleredema in an infant.	a 3-month-old infant with scleredema had associated cytomegalovirus pneumonia with a rapidly fatal course. because differentiation of scleredema from scleredema neonatorum, subcutaneous fat necrosis, and scleroderma may be difficult, histologic diagnosis is important. cetylpyridinium chloride was used successfully as a fixative to demonstrate acid mucopolysaccharides histologically.	1986	3789776
current state and tendencies of antibiotic resistance in hungary.	this survey is based on data for 245 903 isolates reported by public health network laboratories in 1983. facultatively pathogenic gram-negative bacteria comprised two-third of the isolates, and--except escherichia coli--were resistant in a high percent to the most frequently used antibiotics. oxacillin and vancomycin were the most effective against staphylococcus aureus being in 94.7% resistant to penicillin. in contrast to other streptococci, all streptococcus pyogenes strains were sensitive t ...	1986	3811828
human term placenta releases placental protein 10 (pp10) in tissue culture.	placenta, decidua, chorion and amnion were studied to determine the tissue concentration of placental protein 10 (pp10). as measured by radio-immunoassay, all tissues studied were found to contain approximately the same concentration of pp10, in both early and late pregnancy. the release of pp10 was studied in tissue culture, first by using explants of placenta, decidua, amnion and chorion in four preliminary studies. only the placenta released significant amounts of pp10 into the culture medium ...	1986	3811843
canine lymphoma: clinical considerations.		1986	3333887
canine lymphoma: clinical considerations.		1986	3333887
paracentric inversions in man. a french collaborative study. groupe de cytogénéticiens français.	the collection of paracentric inversions ascertained in french cytogenetics laboratories gives a sample of 32 unpublished cases. the carriers seem to have a normal reproductive fitness, without difference between males and females. no cases of aneusomie de recombinaison were found, and it is likely that almost all, and perhaps all cases were ascertained for a cause independent of the inversion. some chromosomes are too frequently affected: 3, 7 and 14; conversely chromosome 2 was never involved, ...	1986	3491573
pharmacokinetics of insulin following intravenous and subcutaneous administration in canines.	studies were conducted to examine the absorption and disposition kinetics of insulin in dogs following intravenous (iv) and subcutaneous (sc) administration of commercial preparations. after iv and sc dosing, the plasma levels were described by models which considered basal insulin level contributions. intersubject variation in the disposition kinetics was small with half-lives of 0.52 +/- 0.05 h and total body clearances of 16.21 +/- 2.08 ml min-1 kg-1. calculated insulin plasma secretion rates ...	1986	3535917
localization and comparative nucleotide sequence analysis of the transforming domain in herpes simplex virus dna containing repetitive genetic elements.	the 7.5-kilobase bamhi e fragment (bamhi-e) of herpes simplex virus type 2 (hsv-2) dna (map position 0.533-0.583) encodes the 144-kda subunit of ribonucleotide reductase and induces the neoplastic transformation of immortalized cell lines. to define the minimal transforming region of bamhi-e, a series of subclones were constructed that spanned the entire fragment. these subclones were assayed for focus formation in rat-2 cells. removal of the promoter region from the viral 144-kda-protein gene l ...	1986	3020562
localization and comparative nucleotide sequence analysis of the transforming domain in herpes simplex virus dna containing repetitive genetic elements.	the 7.5-kilobase bamhi e fragment (bamhi-e) of herpes simplex virus type 2 (hsv-2) dna (map position 0.533-0.583) encodes the 144-kda subunit of ribonucleotide reductase and induces the neoplastic transformation of immortalized cell lines. to define the minimal transforming region of bamhi-e, a series of subclones were constructed that spanned the entire fragment. these subclones were assayed for focus formation in rat-2 cells. removal of the promoter region from the viral 144-kda-protein gene l ...	1986	3020562
differential transient and long-term expression of dna sequences introduced into t-lymphocyte lines.	we have used a protoplast fusion protocol to introduce the genes encoding neomycin phosphotransferase (neo) and chloramphenicol acetyltransferase (cat) into murine and human t-lymphocyte lines. plasmid constructs containing the neo gene under the control of the promoters from the rous sarcoma virus long terminal repeat (rsv ltr), the sv40 early region, or the herpes simplex virus thymidine kinase gene (hsv tk) can stably transform each of three t-cell lines to g-418 resistance. the characteristi ...	1986	3028736
mitomycin c-induced bidirectional transcription from the colicin e1 promoter region in plasmid cole1.	treatment of a colicinogenic culture with mitomycin c induces convergent transcription from two adjacent promoters at the beginning of the colicin e1 gene. s1-mapping and primer extension assays indicate that the mitomycin c-inducible transcripts correspond to colicin e1 mrna (cea mrna) and to a transcript, designated rna-c, that may code for an entry exclusion function. nucleotide sequences that strongly resemble a consensus sequence for lexa protein binding sites span the transcription start p ...	1986	3092859
growth hormone-dependent insulin-like growth factor (igf) binding protein from human plasma differs from other human igf binding proteins.	a growth hormone-dependent binding protein for insulin-like growth factors (igf-i and igf-ii) has been isolated from human plasma. analyzed on sds gels, the preparation contained a major protein band of 53 kda, and a minor band of 47 kda. after transfer to nitrocellulose, both species bound iodinated igf-i, and could be detected using an antibody raised against the purified preparation. in contrast, an igf binding protein purified from human amniotic fluid bound igf-i but was not detectable immu ...	1986	2945561
retinal toxicity of combination antiviral drugs in an animal model.	we investigated the retinal toxicity of various combinations of four antiviral agents in intravitreal injection and vitrectomy infusion solutions in the rabbit. the nontoxic concentration of each individual agent for intravitreal injection and for vitrectomy infusion has previously been described. our results support the concept that concentrations of 200 micrograms/0.1 ml of trifluridine, 400 micrograms/0.1 ml of hydroxyacyclovir, 200 micrograms/0.1 ml of acyclovir and 30 micrograms/0.1 ml of v ...	1987	3123041
the chicken carbonic anhydrase ii gene: evidence for a recent shift in intron position.	the complete nucleotide sequence of the coding region of the chicken carbonic anhydrase ii (ca ii) gene has been determined from clones isolated from a chicken genomic library. the sequence of a nearly full length chicken ca ii cdna clone has also been obtained. the gene is approximately 17 kilobase pairs (kb) in size and codes for a protein that is comprised of 259 amino acid residues. the 5' flanking region contains consensus sequences commonly associated with eucaryotic genes transcribed by r ...	1987	3029691
kappa opioid receptor activation depresses excitatory synaptic input to rat locus coeruleus neurons in vitro.	intracellular recordings were made from neurons of the rat locus coeruleus contained within a brain slice maintained in vitro. when applied to the slice in known concentrations the selective kappa opioid receptor agonist trans-(+)-3,4-dichloro-n-methyl-[2-(1-pyrrolidinyl)cyclohexyl] benzeneacetamide methane sulphonate (u50488) (0.01-1 microm) produced a concentration-dependent depression of the excitatory post-synaptic potential evoked by electrical stimulation of afferent inputs to the locus co ...	1987	3031541
arachidonic acid inhibits phosphate transport by cultured renal cells.	because arachidonic acid and its metabolites are reported to be intracellular messengers of various exogenous stimuli, we studied whether arachidonic acid influences phosphate transport by cultured mouse renal epithelial cells. arachidonic acid, at 10(-7)-10(-4)m, inhibited phosphate transport without influencing cyclic adenosine 3':5'-monophosphate production. nordihydroguaiaretic acid and indomethacin, inhibitors of arachidonic acid metabolism, did not cancel the arachidonic acid-induced inhib ...	1987	3032193
arachidonic acid inhibits phosphate transport by cultured renal cells.	because arachidonic acid and its metabolites are reported to be intracellular messengers of various exogenous stimuli, we studied whether arachidonic acid influences phosphate transport by cultured mouse renal epithelial cells. arachidonic acid, at 10(-7)-10(-4)m, inhibited phosphate transport without influencing cyclic adenosine 3':5'-monophosphate production. nordihydroguaiaretic acid and indomethacin, inhibitors of arachidonic acid metabolism, did not cancel the arachidonic acid-induced inhib ...	1987	3032193
preservation of "peripheral" benzodiazepine receptors: differential effects of freezing on [3h]ro 5-4864 and [3h]pk 11195 binding.	the equilibrium binding constants of [3h]ro 5-4864 (a "peripheral" benzodiazepine receptor ligand) to renal membranes preserved by various freezing techniques were investigated. the bmax for [3h]ro 5-4864 binding to membranes from kidneys preserved as unwashed homogenates stored at -80, -20, or 5 degrees c, whole kidneys stores at -20 or 5 degrees c or as washed homogenate stored at -20 degrees c was significantly decreased (approximately 35%). only when kidneys were frozen intact (using a dry-i ...	1987	3035290
detrimental actions of endogenous fatty acids and their derivatives. a study of ischaemic mitochondrial injury.	functional and structural alterations of myocardial mitochondria were investigated after four conditions of myocardial ischaemia in guinea pig heart: (1) 45 min complete ischaemia, (2) 60 min low-flow anoxic perfusion (0.3 ml/g wet weight per minute) with a modified tyrode solution, (3) as (2) with 0.4 mm palmitic acid added to the perfusate, and (4) as (2) with 0.4 mm oleic acid added. under conditions (1) and (2) the loss of tissue atp (20-30% of aerobic control) and the degree of mitochondria ...	1987	2821981
dopamine denervation of the nucleus accumbens induces a selective increase in the number of delta-opioid binding sites.	selective lesions of dopamine (da) neurons of the nucleus accumbens were made in rats by local injections of 6-hydroxydopamine (6-ohda). seven days after 6-ohda, the binding affinities and capacities at mu-, delta- and kappa-opioid binding sites were determined in the nucleus accumbens by selective labelling techniques. 6-ohda pretreatment caused a significant increase in the number of delta-opioid binding sites (+30%) while the number of mu-, kappa- and total sites was not modified. the affinit ...	1987	2825926
dopamine denervation of the nucleus accumbens induces a selective increase in the number of delta-opioid binding sites.	selective lesions of dopamine (da) neurons of the nucleus accumbens were made in rats by local injections of 6-hydroxydopamine (6-ohda). seven days after 6-ohda, the binding affinities and capacities at mu-, delta- and kappa-opioid binding sites were determined in the nucleus accumbens by selective labelling techniques. 6-ohda pretreatment caused a significant increase in the number of delta-opioid binding sites (+30%) while the number of mu-, kappa- and total sites was not modified. the affinit ...	1987	2825926
galanin-immunoreactive neurons in the guinea-pig small intestine: their projections and relationships to other enteric neurons.	galanin immunoreactivity was observed in nerve cell bodies and nerve fibres, but not in enteroendocrine cells, in the small intestine of the guinea-pig. nerve terminals were found in the myenteric plexus, in the circular muscle, in submucous ganglia, around submucous arterioles, and in the mucosa. lesion studies showed that all terminals were intrinsic to the intestine; those in myenteric ganglia arose from cell bodies in more orally placed ganglia. myenteric nerve cells were also the source of ...	1987	2446770
rapid staining of campylobacter pyloridis.		1987	2447004
phosphorylation, glycosylation, and proteolytic activity of the 52-kd estrogen-induced protein secreted by mcf7 cells.	we have studied the posttranslational modifications of the 52-kd protein, an estrogen-regulated autocrine mitogen secreted by several human breast cancer cells in culture (westley, b., and h. rochefort, 1980, cell, 20:353-362). the secreted 52-kd protein was found to be phosphorylated mostly (94%) on high-mannose n-linked oligosaccharide chains, and mannose-6-phosphate signals were identified. the phosphate signal was totally removed by alkaline phosphatase hydrolysis. the secreted 52-kd protein ...	1987	3543022
phosphorylation, glycosylation, and proteolytic activity of the 52-kd estrogen-induced protein secreted by mcf7 cells.	we have studied the posttranslational modifications of the 52-kd protein, an estrogen-regulated autocrine mitogen secreted by several human breast cancer cells in culture (westley, b., and h. rochefort, 1980, cell, 20:353-362). the secreted 52-kd protein was found to be phosphorylated mostly (94%) on high-mannose n-linked oligosaccharide chains, and mannose-6-phosphate signals were identified. the phosphate signal was totally removed by alkaline phosphatase hydrolysis. the secreted 52-kd protein ...	1987	3543022
protein inclusions produced by the entomopathogenic bacterium xenorhabdus nematophilus subsp. nematophilus.	the entomopathogenic bacterium xenorhabdus nematophilus subsp. nematophilus produces two types of intracellular inclusion bodies during in vitro culture. large cigar-shaped inclusions (designated type 1) and smaller ovoid inclusions (designated type 2) were purified from cell lysates, using differential centrifugation in discontinuous glycerol gradients and isopycnic density gradient centrifugation in sodium diatrizoate. the inclusions, composed almost exclusively of protein, are readily soluble ...	1987	3667532
distribution of immunoreactive inhibin like material in different regions of human prostate.	distribution of immunoreactive inhibin material (ir-ilm) in different regions of human prostate was studied by using specific radio-immunoassay. mean ir-ilm concentrations in the central, peripheral and periurethral zones were 27.3 +/- 9.3, 2.4 +/- 0.7 and 3.0 +/- 1.5 micrograms/mg protein respectively (p central vs periurethral and peripheral less than 0.05).	1987	3688474
the optimum time for neutering surgery of dogs and cats.		1988	3381459
the defect of lymphocyte locomotion in chronic lymphocytic leukaemia: studies of polarization and growth-dependent locomotion.	this paper reports a study of the locomotor behaviour of the lymphocytes from 17 patients with chronic lymphocytic leukaemia (cll). the cells were studied both immediately after separation from blood and after culture for 24 to 48 h with a range of growth activators. cells direct from blood were tested for polarization in fetal calf serum (fcs 20%), phorbol myristate acetate (pma 10(-7)m) and colchicine (10(-5)m). the polarization of lymphocytes from cll patients with high white cell (wbc) count ...	1988	3383451
quantitative carcinogenesis in relation to human exposures.	while carcinogenic potency cannot be expressed in absolute terms, quantitative carcinogenesis nevertheless plays an important role both in assessing cancer risks and in devising effective methods of cancer prevention. attention is drawn to newer methods of prevention based on interference with the carcinogenic process, which is likely to counterbalance, or even to reverse, the anticipated trend of increased cancer incidence resulting from new carcinogens introduced in our environment.	1988	3390839
nonactivated and activated glucocorticoid receptor complexes from human salivary gland adenocarcinoma cell line.	[3h]triamcinolone acetonide glucocorticoid receptor complexes from human salivary gland adenocarcinoma cells (hsg cells) were shown to be activated with an accompanying decrease in molecular weight in intact cells, as analyzed by gel filtration, deae chromatography, the mini-column method and glycerol gradient centrifugation. glucocorticoid receptor complexes consist of steroid-binding protein (or glucocorticoid receptor) and non-steroid-binding factors such as the heat-shock protein of molecula ...	1988	3401512
activation of calpain i and calpain ii: a comparative study using terbium as a fluorescent probe for calcium-binding sites.	the present study demonstrates the activation of calpain i and calpain ii by micromolar levels of terbium and has utilized the enhancement in the fluorescence of protein-bound terbium to study and compare the calcium binding sites of the two enzymes. calpain i and calpain ii were isolated from bovine erythrocytes and brain, respectively. while the rates of activation of calpain i by terbium and calcium are comparable, the rate of activation of calpain ii was much greater in the presence of terbi ...	1988	2847653
[changes in the cyclic nucleotide system of t-lymphocytes in experimental tuberculosis in animals].		1988	2851783
audiological development in south-east asia. thailand health profile.	the otological centre:bangkok unit has been established since march 13, 1985 on the initiation of isa and ifos as one of the planned three centres for each continent of the world. bangkok centre serves the asian countries on the global programme on prevention of hearing impairment and deafness. it was the first of the planned three centres to be established and operative. the centre has already run annual training courses to upgrade oto-audiological knowledge of ent doctors and personnel engaged ...	1988	3187388
effects of corn oil- and fish oil-supplemented diets on phospholipid fatty acid composition of rat liver nuclei.	the effects of dietary fat on fatty acid compositions of phospholipids in liver nuclei were investigated. by feeding a diet containing 5% corn oil or fish oil to rats, the proportions of n -6 or n -3 polyunsaturated fatty acids (pufa), respectively, were increased in phospholipids of the liver nuclei. by feeding a fat-free diet, endogenous pufa were increased. even after feeding the fat-free diet for 40 weeks, the n - 6 still remained at about 10% in both phosphatidylcholine (pc) and phosphatidy ...	1988	3196728