Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| respiratory enzyme systems in symbiotic nitrogen fixation: iii. the dehydrogenase systems of rhizobium trifolii and rhizobium leguminosarum. | 1941 | 16560421 | |
| a medium adapted to the bacteriophage of rhizobium leguminosarum. | 1943 | 16560646 | |
| studies on lyophiled cultures: lyophile storage of cultures of rhizobium leguminosarum. | 1946 | 16561164 | |
| studies on lyophiled cultures; lyophile storage of cultures of rhizobium leguminosarum. | 1946 | 20995934 | |
| some effects of 2,4-d, ddt, and colorado 9 on the bacteria rhizobium leguminosarum frank in the root nodules of the common bean. | 1947 | 20252514 | |
| [nodule bacteria]. | 1950 | 14775505 | |
| [serologic study of rhizobium; application to the problem of competition between strains]. | 1950 | 14800006 | |
| [observations on excretion products of bacterium radicicola and their vitamin properties]. | 1951 | 14859534 | |
| the effect of infection with bacteriophage on the electrokinetic potential of rhizobium leguminosarum. | 1959 | 14409632 | |
| bacterial dissimilation of l-fucose and l-rhamnose. | eagon, r. g. (university of georgia, athens). bacterial dissimilation of l-fucose and l-rhamnose. j. bacteriol. 82:548-550. 1961.-of 33 microorganisms screened for the ability to utilize the methylpentoses, l-fucose and l-rhamnose, 18 species utilized one or both methylpentoses. the majority of the bacterial species capable of utilizing these methylpentoses did so by isomerization of the methylpentoses to the corresponding ketoses followed by phosphorylation of the ketoses. however, the existenc ... | 1961 | 13888945 |
| morphological and cytological observations on gamma rays irradiated rhizobium leguminosarum. | 1963 | 14065833 | |
| [preparation of fluorescent antibodies against rhizobium leguminosarum]. | 1964 | 14337544 | |
| on the cytology and synthetic capacities of natural and artificially produced bacteroids of rhizobium leguminosarum. | 1965 | 5861289 | |
| factors affecting phage-restricting ability in rhizobium leguminosarum strain l4. | 1966 | 5929161 | |
| deoxyribonucleic acid homology and taxonomy of agrobacterium, rhizobium, and chromobacterium. | hybridization experiments were carried out between high molecular weight, denatured, agar-embedded deoxyribonucleic acid (dna) and homologous, nonembedded, sheared, denatured (14)c-labeled dna from a strain of agrobacterium tumefaciens and rhizobium leguminosarum (the reference strains) in the presence of sheared, nonembedded, nonlabeled dna (competing dna) from the same or different nomen-species of agrobacterium, rhizobium, chromobacterium, and several other organisms. percentage of dna homolo ... | 1967 | 4961629 |
| efficiency of isolates of rhizobium leguminosarum (pea) in relation to their dimensions. | 1968 | 5721250 | |
| b12 coenzyme-dependent ribonucleotide reductase in rhizobium species and the effects of cobalt deficiency on the activity of the enzyme. | this investigation revealed that the ribonucleotide reductases in extracts of rhizobium leguminosarum, r. trifolii, r. phaseoli, r. japonicum, and r. meliloti 3doal (ineffective in nitrogen fixation) are dependent upon b(12) coenzyme for activity. rhizobium and certain lactobacillus species are the only two groups of organisms known to contain b(12) coenzyme-dependent ribonucleotide reductases. extracts of cobalt-deficient r. meliloti cells assayed in the presence of optimum b(12) coenzyme showe ... | 1969 | 5780513 |
| mutation to auxotrophy and prototrophy as related to symbiotic effectiveness in rhizobium leguminosarum and r. trifolii. | 1969 | 5798518 | |
| recombination in rhizobium leguminosarum. | 1969 | 5194835 | |
| [interspecific transformations of agrobacterium tumefaciens and rhizobium leguminosarum]. | 1969 | 5384628 | |
| α-aminoadipic acid and α,ɛ-diaminopimelic acid in inoculated pea plants (pisum sativum) and root nodule bacteria (rhizobium leguminosarum). | observations were made on the content of α-aminoadipic acid and α-aminophimelic acid (dap) in pea plants, nodules and rhizobium leguminosarum, strain ht3. the preparations were purified by ion exchange chromatography, qualitative analyses were made by paper chromatography, and quantitative analyses by means of an automatic amino acid analysator. in the whole plant and seeds the content of α-aminoadipic acid soluble in 70% ethanol varied between 10 and 80 μg/g dry weight. the shoot and red nitrog ... | 1969 | 20925682 |
| composition of somatic antigens of rhizobium trifolii and rhizobium leguminosarum. | 1970 | 5497610 | |
| [nucleic acid content in the cells of rhizobium leguminosarum strains with high and low activity]. | 1971 | 5560546 | |
| [lipids of the nodule bacteria rhizobium leguminosarum]. | 1971 | 5567628 | |
| compatibility of rhizobium leguminosarum on peas with fungicides and antibiotic aureofungin used as seed disinfectants. | 1971 | 5148804 | |
| abscisic acid inhibition of root nodule initiation in pisum sativum. | the effect of exogenous abscisic acid (aba) on root nodule formation in pisum sativum cv. alaska was examined. aba supplied to the roots at 1.9×10(-6)m reduced the number of nodules/plant 61% without affecting root or shoot growth. the first noticeable inhibition of nodulation occurred at 3.8×10(-7)m aba. aba at a concentration of 1.9×10(-6)m inhibited neither root hair formation nor infection of root hairs by rhizobium leguminosarum. similar numbers of infection threads penetrated the cortex in ... | 1971 | 24488191 |
| virulent bacteriophage infection of rhizobium leguminosarum. | 1972 | 4502500 | |
| [the effect of aeration on various biochemical indices of rhizobium leguminosarum cultures]. | 1973 | 4771030 | |
| insecticides and soil microorganisms. i. effect of dipterex on the growth of rhizobium leguminosarum and rhizobium trifolii as influenced by temperature, ph and type of nitrogen. | 1973 | 4778605 | |
| [effect of the oxygen regime on the respiration and cytochrome content in rhizobium leguminosarum]. | 1973 | 4782427 | |
| r factor transfer in rhizobium leguminosarum. | 1974 | 4612098 | |
| [study of the population dynamics of rhizobium leguminosarum in soils using immunofluorescence]. | 1974 | 4280495 | |
| [makeup of the cytochromes of the bacteroid rhizobium leguminosarum (vicia faba)]. | 1974 | 4374257 | |
| the flavin content of clovers relative to symbiosis with a riboflavin-requiring mutant of rhizobium trifoli. | a riboflavin-requiring auxotroph of rhizobium trifolii (t1/d-his(r)-15) formed ineffective root nodules on red clover and on two cultivars of subterranean clover, but produced almost fully effective nodules on several other cultivars of subterranean clover. fluorescence and bioassay measurements of the flavin content of the roots and shoots of these cultivars revealed no differences between cultivars which could be correlated with the differences in symbiotic response. the concentration of flavi ... | 1974 | 16658676 |
| identification of the rhizobium strains in pea root nodules using genetic markers. | pea plants were inoculated jointly with pairs of genetically marked strains of rhizobium leguminosarum. out of 297 modules examined 56 contained both inoculant strains. the ratios of the strains in the inoculum did not affect the frequencies of mixed nodules. generally one of the strains consistently occupied the majority of the nodules and ithe mixed nodules comprised the majority of bacteria. transfer of the p-group r factor, rp4, between certain strains of rhizobium within mixed nodules was d ... | 1975 | 1141859 |
| insecticides and soil microorganisms. iii. fate of 14c-labelled dipterex as affected by two nodule-forming rhizobium spp. and roots of their respective leguminous host plants. | chromatographic analysis led to the identification of monomethyl- and dimethyl-phosphates as metabolites resulting from the enzymatic degradation of 14c-labelled dipterex in the buffer solutions and root tissues of broad bean and clover plants, as well as in the culture media of rhizobium leguminosarum and rhizobium trifolii. the formation of 14co2 from rhizobial cultures containing radioactive dipterex suggests that some of the liberated methanol groups (during breakdown of dipterex) are oxidat ... | 1975 | 1220485 |
| efficiency of rhizobium leguminosarum as affected by certain herbicides and nematocides. | 1975 | 1243844 | |
| enzymes of ammonia assimilation in rhizobium leguminosarum bacteroids. | the activities of the following enzymes were studied in connection with dinitrogen fixation in pea bacteroids: glutamine synthetase(l-glutamate: ammonia ligase (adp-forming)(ec 6.3.1.2)(gs); glutamate dehydrogenase (nadp+)(l-glutamate: nadp+ oxidoreductase (deaminating)(ec 1.4.1.4)(gdh); glutamate synthase (l-glutamine: 2-exeglutarate aminotransferase (nadph-oxidizing))(ec 2.6.1.53)(gogat). gs activity was high throughout the growth of the plant and gogat activity was always low. it is unlikely ... | 1975 | 238731 |
| fructose 1,6-bisphosphate aldolase activity of rhizobium species. | fdp aldolase was found to be present in the cell-free extracts of rhizobium leguminosarum, rhizobium phaseoli, rhizobium trifolii, rhizobium meliloti, rhizobium lupini, rhizobium japonicum and rhizobium species from arachis hypogaea and sesbania cannabina. the enzyme in 3 representative species has optimal activity at ph 8.4 in 0.2m veronal buffer. the enzyme activity was completely lost by treatment at 60 degrees c for 15 min. the km values were in the range from 2.38 to 4.55 x 10(-6)m fdp. met ... | 1975 | 283 |
| [symbiotic efficiency in spontaneous mutants of rhizobium legumino-sarum resistant to streptomyocin, spectinomycin, or kanamycin]. | symbiotic effectiveness of 45 mutant strains selected from four wild effective strains of rhizobium leguminosarum for resistance to streptomycin, spectinomycin or kanamycin was determined on vicia faba. loss of effectiveness occurred in twenty of these mutants; distribution of ineffective mutants was uniform among the three types of antibiotic resistant mutants but varied with the parent strain from which mutants have been derived. | 1975 | 125155 |
| a comparison of dna from free living and endosymbiotic rhizobium leguminosarum (strain pre). | 1. bacteroids of rhizobium leguminosarum (strain pre) purified from root nodules of pisum sativum (var. 'rondo') by the standard procedure of differential centrifugation contained considerable contamination of mitochondrial material. this could be removed by incubation of the bacteroid preparation with 1 m kcl/1% deoxycholate. 2. the dna content of bacteroid cells of r. leguminosarum was found to have increased about three fold in comparison with the dna content of free living r. leguminosarum b ... | 1975 | 172157 |
| studies on phage 1p receptors in rhizobium trifolii and rhizobium leguminosarum. | the rate of phage 1p attachment to rhizobium cell walls was increased in the presence of ethylenediaminetetraacetic acid (edta). on the other hand the rate of adsorption of phage 1p to the triton -- insoluble cell walls was diminished. the subsequent treatment of cell walls with 2% triton x-100 and 5mm edta caused a more substantial decline of the phage inactivating capacity. lipopolysaccharides (lps) isolated from the sensitive strains, contrary to those from phage-resistant mutants, inactivate ... | 1975 | 813494 |
| discrimination of rhizobium japonicum, rhizobium lupini, rhizobium trifolii, rhizobium leguminosarum and of bacteroids by uptake of 2-ketoglutaric acid, glutamic acid and phosphate. | rhizobium strains (one each of rh. japonicum, rh. lupini, rh. leguminosarum) take up 2-ketoglutaric acid in general much faster and from lower concentrations in the medium than strains of escherichia coli, bacillus subtilis and chromobacterium violaceum. a strain of enterobacter aerogenes, however, is more similar to some rhizobium strains. the same strains of rhizobium take up also phosphate much faster and from lower concentrations than the other bacteria tested. 4 strains of rh. lupini proved ... | 1976 | 818969 |
| improved method for preparing anaerobic bacteroid suspensions of rhizobium leguminosarum for the acetylene reduction assay. | a method using ethylenediaminetetraacetate (edta) and toluene-treated pea bacteroid suspensions for the acetylene reduction assay is described. the high level of acetylene reduction by these bacteroids is comparable to that of intact plants. reproducibility of the edta-toluene treatment is, on the average, within 5%. preliminary experiments with soybeans indicate that the edta-toluene method might be applicable to other legumes as well. | 1976 | 820257 |
| chromosomal recombination and mapping in rhizobium leguminosarum. | 1976 | 826827 | |
| properties of plasmids constructed by the in vitro insertion of dna from rhizobium leguminosarum or proteus mirabilis into rp4. | plasmids have been constructed by insertion of dna from rhizobium leguminosarum or proteus mirabilis into rp4 (an r factor of group p). such recombinant plasmids retain the wide host range of the parental plasmid, being as efficiently transmissible as the unmodified rp4 and are stably maintained in rapidly growing cultures. the recombinant plasmids, even though each contained a dna sequence absolutely identical with that of the host strain, are no more efficient at mobilizing the transfer of chr ... | 1976 | 787766 |
| mixed inoculations with effective and ineffective strains of rhizobium leguminosarum. | 1976 | 939736 | |
| binding of pea lectins to a glycan type polysaccharide in the cell walls of rhizobium leguminosarum. | 1977 | 838048 | |
| zeatin ribonucleosides in the transfer ribonucleic acid of rhizobium leguminosarum, agrobacterium tumefaciens, corynebacterium fascians, and erwinia amylovora. | until recently, the presence in transfer ribonucleic acid (trna) of the hydroxylated cytokinin ribosylzeatin [n6-(4-hydroxy-3-methylbut-2-enyl)adenosine]was thought to be unique to higher plants. this extension of work from several laboratories indicates the presence of 2-methylthioribosylzeatin in the trna of the plant-associated bacteria rhizobium leguminosarum, agrobacterium tumefaciens, and corynebacterium fascians, but not in that of erwinia amylovora. this cytokinin has the cis configurati ... | 1977 | 893341 |
| [electrophoretic characteristics of the protein composition of mutant forms of pea nodular bacteria]. | a water-soluble protein fraction was studied by gel electrophoresis in the parent and mutant forms of the nodule bacterium rhizobium leguminosarum. the composition of this fraction changed under the action of ntg. changes in the composition of protein components in the mutants as compared with that of the parent strain are caused presumably by their modified specificity. | 1977 | 909477 |
| the effect of ammonium nitrate on the synthesis of nitrogenase and the concentration of leghemoglobin in pea root nodules induced by rhizobium leguminosarum. | the effects of nh4no3 on the development of root nodules of pisum sativum after infection with rhizobium leguminosarum (strain pre) and on the nitrogenase activity of the bacteroids in the nodule tissue were studied. the addition of nh4no3 decreased the nitrogenase activity measured on intact nodules. this reduction of nitrogen fixation did not result from a reduced number of bacteroids or a decreased amount of bacteroid proteins per gram of nodule. the synthesis of nitrogenase, measured as the ... | 1978 | 623788 |
| membrane energization in relation with nitrogen fixation in azotobacter vinelandii and rhizobium leguminosarum bacteroids. | nitrogen fixation in a. vinelandii and r. leguminosarum bacteroides shows identical characteristics with respect to the dependence on membrane energization, the sensitivity to uncouplers, the atp/adp-ratio, and the dependences on flavodoxinhydroquinone as electrondonor. although we have been successful in preparing inside-out vesicles which can be energized, attempts to couple these membranes to n2-ase were still unsuccessful. one of the major problems could be the failure to energize these vesi ... | 1978 | 667180 |
| involvement of the cytoplasmic membrane in nitrogen fixation by rhizobium leguminosarum bacteroids. | 1. the nitrogen-fixing efficiency of freshly prepared suspensions of rhizobium leguminosarum bacteroids from pea root nodules was considerably enhanced by addition of bovine serum albumin. evidence was found that during preparation of bacteroids the cell membrane is exposed to the uncoupling effect of free fatty acids and to plant phospholipase d activity. both effects could be counteracted by bovine serum albumin. 2. a technique was developed by which concentrations of free o2 and nitrogenase a ... | 1978 | 668685 |
| trifluralin effect on pisum--rhizobium relationship. | trifluralin inhibited root and shoot elongation of pisum sativum plant and caused isodiametric increase in cell volume of both tissues. the water content of the plant was not affected. the weedicide inhibited also growth and o2 uptake of rhizobium leguminosarum, isolated from pisum plant. reduction in the nitrogen content of pisum tissues was noticed, and this may be attributed to the inhibition of nodule formation. variations in the free and protein-amino acids were observed in the plant tissue ... | 1978 | 726711 |
| some aspects of fermentation of rhizobium leguminosarum with reference to economy of ingredients in the substrate. | incremental feeding of nutrients was found to be beneficial in mass cultivation of r. leguminosarum. similarly, semi-continuous fermentation of r. leguminosarum up to 4 days was also found to be useful in maintaining the viable number of cells. | 1978 | 373307 |
| diversity of rhizobium leguminosarum in the palouse of eastern washington. | serology, antibiotic resistance, litmus milk reaction, and ability to fix nitrogen in peas were methods employed in studying the diversity of rhizobium leguminosarum. the two serogroups identified were related to the position on the soil slope where isolated were taken. | 1978 | 16345331 |
| interdependence of nitrogen nutrition and photosynthesis in pisum sativum l: i. effect of combined nitrogen on symbiotic nitrogen fixation and photosynthesis. | photosynthesis, primary productivity, n content, and n(2) fixation were determined as a function of applied nh(4) (+) in peas (pisum sativum l. cv. alaska) which were inoculated or not inoculated with rhizobium leguminosarum. cabon dioxide exchange rate (cer) increased 10-fold, total n content 7-fold, and total dry weight 3-fold in 26-day-old uninoculated plants as applied nh(4) (+) was increased from 0 to 16 millimolar. in inoculated plants of the same age cer and dry weight were maximal at 2 m ... | 1978 | 16660450 |
| interdependence of nitrogen nutrition and photosynthesis in pisum sativum l: ii. host plant response to nitrogen fixation by rhizobium strains. | physiological responses to infection by strains of rhizobium leguminosarum which differed in their capacity to reduce n(2) were determined in 26-day-old pea plants (pisum sativum l. cv. alaska) grown under uniform environmental conditions in the absence of combined n. the highest n(2) reduction rates, calculated from h(2) evolution and c(2)h(2)-dependent c(2)h(4) production measurements, were approximately 6-fold greater than the lowest. higher n(2) fixation rates were associated with greater co ... | 1978 | 16660451 |
| host-symbiont interactions: iii. purification and partial characterization of rhizobium lipopolysaccharides. | the lipopolysaccharides of three strains each of rhizobium leguminosarum, r. phaseoli, and trifolii have been purified and partially characterized. the last step in the purification procedure is gel filtration column chromatography using sepharose 4b with an elution buffer consisting of ethylenediaminetetraacetic acid and triethylamine. each of the lipopolysaccharides reported in this paper elutes as a symmetrical peak in the partially included volume of this sepharose 4b column. the ratio of 2- ... | 1978 | 16660637 |
| variation in nitrogenase and hydrogenase activity of alaska pea root nodules. | hydrogenase activity of root nodules in the symbiotic association between pisum sativum l. and rhizobium leguminosarum was determined by incubating unexcised nodules with tritiated h(2) and measuring tissue hto. hydrogenase activity saturated at 0.50 millimolar h(2) and was not inhibited by the presence of 0.10 atmosphere c(2)h(2), which prevented h(2) evolution from nitrogenase. total h(2) production from nitogenase was estimated as net h(2) evolution in air plus h(2) exchange in 0.10 atmospher ... | 1979 | 16660819 |
| environmental and genotypic effects on the respiration associated with symbiotic nitrogen fixation in peas. | estimated values for the respiration associated with symbiotic nitrogen fixation in pisum sativum l. were independent of irradiance, temperature, plant age, and co(2) concentration, despite large variation in the total rates of c(2)h(2) reduction and root + nodule respiration. similar values were also found in phaseolus vulgaris l., vicia faba l. and glycine max (l.) merr. among all combinations of four pisum cultivars with four rhizobium leguminosarum inoculants only the plant genotype signific ... | 1979 | 16660833 |
| expression of escherichia coli tryptophan operon in rhizobium leguminosarum. | rp4-trp hybrid plasmid containing escherichia coli whole tryptophan operon was conjugatively transferred from e. coli to rhizobium leguminosarum strains carrying mutations in different trp genes, converting their trp- phenotype to trp+. that the phenotype change of the r. leguminosarum cells was due to the presence of the e. coli tryptophan operon was verified by the isolation of rp4-trp hybrid plasmid from the r. leguminosarum conjugant cells, and by re-transfer of rp4-trp plasmid by conjugatio ... | 1979 | 375025 |
| host dependence of rp1-specified resistance to ampicillin: differential expression in escherichia coli and rhizobium leguminosarum. | rhizobium leguminosarum l4 is able to serve as a host for the plasmid rp1. properties of r. leguminosarum [rp1] plasmid carrier suggest that the expression of rp1-coded apr gene(s) is inhibited in this host, although the determinants of transfer and resistance to kanamycin and tetracycline are expressed. this system exemplifies a differential expression of plasmid genes in a new host. | 1979 | 391648 |
| development of the nitrogen-fixing and protein-synthesizing apparatus of bacteroids in pea root nodules. | some aspects of root nodule development of pisum sativum inoculated with rhizobium leguminosarum were examined. 1. nitrogenase activity (measured as acetylene reduction) appears to be preceded by leghemoglobin synthesis (measured immunologically). 2. syntheses of component i and component ii of nitrogenase are not strictly coordinated. synthesis of component i starts before component ii. 3. plant and bacteroid protein synthesis (measured by [35s]sulfate labeling) in root nodules declines rapidly ... | 1979 | 454614 |
| the involvement of the membrane potential in nitrogen fixation by bacteroids of rhizobium leguminosarum. | 1979 | 467678 | |
| [effect of indolylacetic acid on formation of bacteroid forms of rhizobium leguminosarum]. | the purpose of this work was to study the effect of indolylacetic acid (iaa) on the strains of rhizobium leguminosarum, effective and noneffective with respect to symbiotic nitrogen fixation (l4 and 245a, and 14--73, respectively). iaa at a concentration of 50 mcg/ml and higher inhibited the growth of the bacterium, temporarily delayed celular division, and induced intensive formation of elongated bacteroid-like cells, predominantly y-shaped or having a clavate shape. many bacteroid-like cells w ... | 1979 | 502911 |
| salt tolerance of rhizobium species in broth cultures. | salt tolerance of five rhizobia strains was examined in broth cultures. five levels of nacl concentration were used and the optical density was taken as a measure for the vigour of bacterial growth. rhizobium leguminosarum and r. meliloti were tolerant to high levels of salinity and growth curves in saline broth showed a similar pattern to the control level. rhizobium japonicum, cowpea rhizobium, and r. trifolii were intolerant to salt and showed a strong growth retardation with increasing salt ... | 1979 | 547498 |
| on the efficiency of oxidative phosphorylation in membrane vesicles of azotobacter vinelandii and of rhizobium leguminosarum bacteroids. | 1979 | 223842 | |
| strain-specific antigens in rhizobium leguminosarum. | fifty strains of rhizobium leguminosarum, isolated from five species of host plant (pisum sativum, p. arvense, vicia sativa, v. faba, and a lathyrus sp.) were examined for the presence of strain-specific somatic antigens by immune-diffusions against 13 antisera. thirty eight strains (76 per cent) were found to belong to the same sero-group and were serologically indistinguishable from each other, but four of these strains also exhibited non-reciprocal cross reactivity with other antisera. in con ... | 1979 | 89766 |
| comparative properties of glutamine synthetases i and ii in rhizobium and agrobacterium spp. | some properties of glutamine synthetase i (gsi) and gsii are described for a fast-growing rhizobium sp. (rhizobium trifolii t1), a slow-growing rhizobium sp. (rhizobium japonicum usda 83), and agrobacterium tumefaciens c58. gsii of the fast-growing rhizobium sp. and gsii of the agrobacterium sp. were considerably more heat labile than gsii of the slow-growing rhizobium sp. as previously shown in r. japonicum 61a76, gsi became adenylylated rapidly in all species tested in response to ammonium. gs ... | 1980 | 6107288 |
| typing of rhizobium with two different phage dilutions. | the sensitivity of 223 rhizobium strains to 28 phages in concentration 4xrtd and 0.4xrtd was compared. depending on their sensitivity to the different phages the strains were divided into three groups: 1. rhizobium trifolii strains, rhizobium leguminosarum for pea, vetch and horse bean and rhizobium phaseoli, 2. r. trifolii strains and r. leguminosarum for pea and vetch, 3. r. meliloti strains, r. lupini and r. meliloti mutants. the use of 4xrtd distinguished 50 different phage types among the 2 ... | 1980 | 6164253 |
| [effectiveness of symbiotic n2-fixation in leguminous plants, as affected by inoculation with rhizobia, by substrate, n-fertilizing, and 14c-sucrose application (author's transl)]. | cultivation experiments (mitscherlich-vessels, quartz sand, 15n-labelled soil, 15n-fertilizer) showed, that various strains of rhizobium lupini (white and yellow lupines) and of rhizobium leguminosarum (field beans and peas) induced a different n2-fixation of the inoculated plants, the most effective rhizobium strains being 367a, cz, t3, 271 (rh. lupini), and azotogen (rh, leguminosarum). yellow lupines and field bean plants were supplied with n2 from the air considerably better than white lupin ... | 1980 | 6252716 |
| polyol metabolism by rhizobium trifolii. | in rhizobium trifolii 7000, the polyols myo-inositol, xylitol, ribitol, d-arabitol, d-mannitol, d-sorbital, and dulcitol are metabolized by inducible nicotinamide adenine dinucleotide-dependent polyol dehydrogenases. five different polyol dehydrogenases were recognized: inositol dehydrogenase, specific for inositil; ribitol dehydrogenase, specific for ribitol; d-arabitol dehydrogenase, which oxidized d-arabitol, d-mannitol, and d-sorbitol; xylitol dehydrogenase, which oxidized xylitol and d-sorb ... | 1980 | 6767702 |
| expression of large plasmids in the endosymbiotic form of rhizobium leguminosarum. | isolated plasmid dna from rhizobium leguminosarum was hybridised with cellular rna from broth-cultured bacteria and endosymbiotic bacteroids. from these hybridisation, experiments it is concluded that plasmid genes are strongly expressed in bacteroids and only weakly or not at all in bacteria. from the hybridisation of plasmid dna with the cloned structural nif genes of klebsiella pneumoniae it is shown that at least part of the nif genes are located on a plasmid. | 1980 | 7001365 |
| short-term effect of ammonium chloride on nitrogen fixation by azotobacter vinelandii and by bacteroids of rhizobium leguminosarum. | 1980 | 6928406 | |
| the effect of waterlogging on the synthesis of the nitrogenase components in bacteroids of rhizobium leguminosarum in root nodules of pisum sativum. | 1980 | 6930267 | |
| turnover of nitrogenase and leghemoglobin in root nodules of pisum sativum. | turnover rates of the two nitrogenase components and leghemoglobin in root nodules of pea plants nodulated with rhizobium leguminosarum were determined with three different methods: 1, kinetics of 35s incorporation into protein; 2, pulse-chase experiments; 3, chloramphenicol inhibition of bacteroid protein synthesis. methods 1 and 3 revealed that the turnover rates of the two nitrogenase components and leghemoglobin are identical to the average rate of bacteroid and plant nodule protein turnover ... | 1980 | 6938244 |
| infection and nodulation of clover by nonmotile rhizobium trifolii. | nonmotile mutants of rhizobium trifolii were isolated to determine whether bacterial motility is required for the infection and nodulation of clover. the nonmotile mutants were screened for their ability to infect and nodulate clover seedlings in fahraeus glass slide assemblies, plastic growth pouches, and vermiculite-sand-filled clay pots. in each system, the nonmotile mutants were able to infect and nodulate clover. | 1980 | 7364722 |
| rhizobium leguminosarum mutants incapable of normal extracellular polysaccharide production. | mutants of rhizobium leguminosarum which are deficient in normal polysaccharide production have been isolated and characterized. a correlation between diminished production of extracellular polysaccharide and reduced infection and nodulation efficiency has been observed. | 1980 | 7364730 |
| dynamics of rhizobium competition for nodulation of pisum sativum cv. afghanistan. | nodulation of pisum sativum cv. afghanistan by rhizobium leguminosarum strain tom can be blocked by the nonnodulating r. leguminosarum strain pf2. this system was used to study the dynamics of rhizobial binding to seedling root surfaces. appreciable numbers of 35s-labeled rhizobia bound to the root surface within 2h after inoculation. pf2 colonized the root surfaces earlier and in greater numbers that did tom. radioactive extracellular polysaccharides and lipopolysaccharides from either organism ... | 1980 | 7378950 |
| promotion of infection thread formation by substances from rhizobium. | an extrinsic substance (es-6000) was isolated from the periplasmic space of rhizobium trifolii (strain 4s) cells by osmotic shock, using a high-density sucrose solution. this substance promoted infection thread formation in root hairs of white clover when inoculated together with the infectious strain (4s). however, es-6000 obtained from another rhizobial species and from strain a1, which is a noninfectious mutant strain obtained from strain 4s, did not have this effect. the promoter in the es-6 ... | 1980 | 16345501 |
| interaction between a non-nodulating and an ineffective mutant of rhizobium trifolli resulting in effective (nitrogen-fixing) nodulation. | a noninvasive (non-nodulating) mutant of rhizobium trifolii when mixed with an ineffective (non-nitrogen-fixing) mutant gives rise to effective (nitrogen-fixing) nodules. | 1980 | 16345518 |
| use of two-dimensional polyacrylamide electrophoresis to demonstrate that putative rhizobium cross-inoculation mutants actually are contaminants. | two-dimensional polyacrylamide electrophoresis was used to determine that mutants of rhizobium trifolii dt6, claimed to be capable of effectively nodulating soybeans, were actually rhizobium japonicum 110 contaminants isolated from the parent dt6 culture. | 1980 | 16345520 |
| scanning electron microscopy of rhizobium trifolii infection sites on root hairs of white clover. | white clover root hairs which were inoculated with rhizobium trifolii 4s (infectious strain) contained infection threads which were observed by light microscopy and scanning electron microscopy. three morphological types of root hairs retaining infection threads were recognized. the bacteria were strongly attached between the surfaces of two plant cell walls as follows: between surfaces of a root hair tip curled back on itself, between a protuberance from a root hair and its cell surface, or bet ... | 1980 | 16345672 |
| interactions between rhizobia and lectins of lentil, pea, broad bean, and jackbean. | a quantitative method was developed to measure the binding of fluorescent-labeled lentil (lens esculenta moench), pea (pisum sativum l.), broad bean (vicia faba l.), and jackbean (canavalia ensiformis l., dc.) lectins to various rhizobium strains. lentil lectin bound to three of the five rhizobium leguminosarum strains tested. the number of lentil lectin molecules bound per r. leguminosarum 128c53 cell was 2.1 x 10(4). lentil lectin also bound to r. japonicum 61a133. pea and broad bean lectins b ... | 1980 | 16661328 |
| host-symbiont interactions : v. the structure of acidic extracellular polysaccharides secreted by rhizobium leguminosarum and rhizobium trifolii. | the sequence of the glycosyl residues and the anomeric configurations of the glycosyl linkages of the acidic polysaccharides secreted by rhizobium leguminosarum 128c53, rhizobium leguminosarum 128c63, rhizobium trifolii na30, and rhizobium trifolii 0403 have been determined. all four polysaccharides were found to have the following glycosyl repeating-unit structure, where galactosyl is gal, glucosyl is glc, glucuronosyl is glca, and pyruvyl is pyr: [formula: see text] each of the glycosyl residu ... | 1981 | 16661681 |
| nitrogen stress and apparent photosynthesis in symbiotically grown pisum sativum l. | pea plants (pisum sativum l. cv. alaska) were inoculated individually with one of 15 rhizobium leguminosarum strains and grown under uniform environmental conditions in the absence of combined n. differences in effectiveness of the rhizobium strains produced plants with differing rates of whole plant apparent n(2) fixation and total n content at the same morphological stage of development. plants were analyzed to determine interactions between n(2) fixation, n allocation, apparent photosynthesis ... | 1981 | 16661907 |
| transient susceptibility of root cells in four common legumes to nodulation by rhizobia. | root cells of four common legumes were found to remain susceptible to nodulation by rhizobia for only a short period of time. delayed inoculation experiments conducted with these legume hosts indicated that the initially susceptible region of the root became progressively less susceptible if inoculations were delayed by a few hours. profiles of the frequency of nodule formation relative to marks indicating the regions of root and root hair development at the time of inoculation indicated that no ... | 1981 | 16662065 |
| c(4)-dicarboxylate transport mutants of rhizobium trifolii form ineffective nodules on trifolium repens. | mutants of rhizobium trifolii strain 7012 defective in c(4)-dicarboxylate transport were isolated by using a selective procedure based on ph indicator media. the mutant strains cr7098 and cr7099 failed to grow on or transport succinate, fumarate, or malate, but grew at wild-type rates on several other carbon sources. the c(4)-dicarboxylate transport system was inducible in strain 7012, but was expressed constitutively in four out of five succinate-positive revertants of strain cr7098. in the fif ... | 1981 | 16593058 |
| effect of nh4+ on nitrogenase activity in nodule breis and bacteroides from pisum sativum l. | nodule breis and bacteroid preparations were made from pisum sativum l. (cv. trapper) inoculated with a single strain of rhizobium leguminosarum. the detached nodules were triturated under helium flow. the resultant breis could support c2h2 reduction in n-tris[hydroxymethyl]methyl-2-aminoethane-sulfonic acid buffer (tes) without any additions for over an hour. nh4+ was found to inhibit c2h2 reduction and h2 evolution. the inhibition was not dependent on the counterion and was evident immediately ... | 1981 | 6938248 |
| large plasmids of fast-growing rhizobia: homology studies and location of structural nitrogen fixation (nif) genes. | a single large plasmid was isolated from multiplasmid-harboring strains rhizobium leguminosarum 1001 and r. trifolii 5. these single plasmids, as well as the largest plasmid detectable in r. phaseoli 3622, hybridized with part of the nif structural genes of klebsiella pneumoniae. in contrast, the plasmids of r. meliloti strains v7 and l5-30 did not show hybridization with the nif genes of k. pneumoniae, indicating that these genes might be located either on the chromosome or on a much larger pla ... | 1981 | 7009569 |
| structure of rigid-layer of rhizobium cell wall. i. purification on the peptidoglycan from the cellulose microfibrils. | the bag shaped peptidoglycan layer of rhizobium cell wall was isolated from intact cells after treatment with sodium dodecylsulfate and trypsin, chymotrypsin or pepsin digestion. results of chemical analysis of acid hydrolyzed peptidoglycan revealed beside two amino sugars: glucosamine and muramic acid, three major amino acids; alanine, glutamic acid and 2,6-diaminopimelic acid and also significant amount of glucose. evidence were provided that the polyglucose found in peptidoglycan preparations ... | 1981 | 6179395 |
| growth-phase-dependent immunodeterminants of rhizobium trifolii lipopolysaccharide which bind trifoliin a, a white clover lectin. | the lipopolysaccharide (lps) from rhizobium trifolii 0403 was isolated at different stages of growth and was examined for its (i) ability to bind a white clover lectin (trifoliin a), (ii) immunochemical properties, and (iii) composition. there was significantly more binding of trifoliin a to purified lps and cells in the early stationary phase than to cells in the exponential phase. immunofluorescence and enzyme-linked immunosorbent assays indicated that new antigenic determinants of the lps app ... | 1981 | 6170630 |
| [bacterial population dynamics in a soil--plant system]. | the dynamics of rhizobium leguminosarum and arthrobacter crystallopoietes populations introduced into soil at different levels of density was studied in a zone near barley roots. microbial life with a high rate of growth was found only at the root surface. for a. crystallopoietes, the ultimate bacterial incidence at the root surface was found to depend on the original level of population density. for rh. leguminosarum, the ultimate incidence was shown to reach an identical level irrespective of ... | 1981 | 7219211 |
| succinate transport in rhizobium leguminosarum. | the transport of succinate was studied in an effective streptomycin-resistant strain of rhizobium leguminosarum. high levels of succinate transport occurred when cells were grown on succinate, fumarate, or malate, whereas low activity was found when cells were grown on glucose, sucrose, arabinose, or pyruvate as the sole carbon source. because of the rapid metabolism of succinate after transport into the cells, a succinate dehydrogenase-deficient mutant was isolated in which intracellular succin ... | 1981 | 7287623 |
| the map position of sym-plasmid regions expressed in the bacterial and endosymbiotic form of rhizobium leguminosarum. | 1982 | 6179109 | |
| restriction endonuclease mapping of a rhizobium leguminosarum sym plasmid. | 1982 | 6285400 | |
| on the operon structure of the nitrogenase genes of rhizobium leguminosarum and azotobacter vinelandii. | the transcription of the nitrogenase genes in rhizobium leguminosarum was studied by analysing total cellular rna from bacteroids for the presence of nitrogenase messenger rna. the rna was separated by agarose gel electrophoresis and blotted onto nitrocellulose filters. messenger rna for nitrogenase was detected by hybridization with probes derived from plasmid psa30, a recombinant plasmid carrying the nitrogenase genes of klebsiella pneumoniae. in the same way nitrogenase mrna was detected in r ... | 1982 | 6289264 |
| uptake hydrogenase activity and atp formation in rhizobium leguminosarum bacteroids. | the role of uptake hydrogenase was studied in rhizobium leguminosarum bacteroids from the nodules of pisum sativum l. cv. homesteader. uptake hydrogenase activity, measured by the 3h2 uptake method, was dependent on o-consumption and was similar to h2 uptake measured by gas chromatography. km for o2 of 0.0007 atm (0.0709 kpa) and a km for h2 of 0.0074 atm (0.7498, kpa) were determined. h2 increased the rate of endogenous respiration by isolates with uptake hydrogenase (hup+) but had no effect on ... | 1982 | 7047503 |
| regulation of tryptophan genes in rhizobium leguminosarum. | twelve tryptophan auxotrophs of rhizobium leguminosarum were characterized biochemically. they were grown in complex and minimal media with several carbon sources, in both limiting and excess tryptophan. missing enzyme activities allowed assignment of all mutant to the trpe, trpd, trpb, or trpa gene, confirming earlier results with the same mutants (johnston et al., mol. gen. genet. 165:323-330, 1978). in regulatory experiments, only the first enzyme of the pathway, anthranilate synthase, respon ... | 1982 | 7061383 |