Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [polysaccharides produced by rhizobium strains; oligosaccharides produced by acid hydrolysis of rhizobium japonicum polysaccharides]. | 1958 | 13537427 | |
| the effect of cobalt on the growth of rhizobium japonicum. | 1960 | 13763818 | |
| [study of the polysaccharides produced by strains of rhizobium. structure of 2 trisaccharide from rhizobium japonicum]. | 1960 | 14415498 | |
| growth inhibition by biotin in a strain of rhizobium japonicum. | 1963 | 14066493 | |
| quick serological method of classifying strains of rhizobium japonicum in nodules. | means, ura m. (u.s. department of agriculture, beltsville, md.), herbert w. johnson, and r. a. date. quick serological method of classifying strains of rhizobium japonicum in nodules. j. bacteriol. 87:547-553. 1964.-a new method of classifying strains of rhizobium japonicum serologically by utilizing the contents of nodules as antigens is described. the method differs from standard procedures only in the preparation of antigens, but it is less time-consuming. agglutination reactions of culture a ... | 1964 | 14127569 |
| preparation and some properties of a soluble nitrate reductase from rhizobium japonicum. | 1964 | 14194853 | |
| influence of gamma radiation on the nitrogen fixation activity of rhizobium japonicum in symbiosis with two varieties of soy-beans. | 1964 | 14211345 | |
| the enzymatic synthesis of a (beta-i,2-)-linked glucan by an extract of rhizobium japonicum. | 1964 | 14220709 | |
| minimal antigenic constitution of 28 strains of rhizobium japonicum. | 1965 | 14290956 | |
| nitrogen metabolism of some strains of rhizobium japonicum having different nodulating capacities. | 1965 | 5879501 | |
| production of the soybean-chlorosis toxin by rhizobium japonicum in pure culture. | 1965 | 16656177 | |
| some effects of medium composition and metabolic intermediates on biotin inhibition in a strain of rhizobium japonicum. | 1967 | 6039199 | |
| correlation of physiological characteristics with nodulating ability in rhizobium japonicum. | 1967 | 6068177 | |
| [the role of rhizobium japonicum in the n-metabolism of soy beans under the varied soil conditions of bulgaria]. | 1967 | 5632929 | |
| [enzymes intervening in the formation of nucleotide-sugars in rhizobium japonicum]. | 1967 | 4962178 | |
| fluorescent-antibody approach to study of rhizobia in soil. | application of fluorescent-antibody (fa) techniques to the study of rhizobia as free-living soil bacteria was explored. antiserum to a particular strain of rhizobium japonicum proved specific in both agglutination and fa tests. within the r. japonicum group, 2 of 12 strains were stained by the conjugate and these fluoresced brightly; all others were entirely negative. fa tests were negative for 7 strains of r. meliloti, 9 strains of r. leguminosarum, 9 strains of r. trifolii, 6 strains of r. pha ... | 1968 | 4174666 |
| alcohol precipitation as a method for collecting extracellular material from rhizobium japonicum. | 1968 | 5636456 | |
| thermostability of antigens associated with serotype of rhizobium japonicum. | the antigens associated with serologically distinct strains of rhizobium japonicum were found to differ in heat sensitivity. cell preparations from 4 out of 12 strains retained agglutinability, and 1 out of 5 retained antigenicity after they were heated to 120 c. antigenicity was reduced in most strains after heating to 100 c for 30 min, but agglutinability was not affected by this treatment. this suggests that the antigens are protein-polysaccharide-lipid complexes described for o-type antigens ... | 1968 | 5645406 |
| nitrogen, energy and vitamin nutrition of rhizobium japonicum. | 1968 | 5702037 | |
| glucose catabolism in rhizobium japonicum. | glucose catabolism in rhizobium japonicum atcc 10324 was investigated by the radiorespirometric method and by assaying for key enzymes of the major energy-yielding pathways. specifically labeled glucose gave the following results for resting cells, with values expressed as per cent (14)co(2) evolution: c-1=59%, c-2=51%, c-3=45%, c-4=59%, and c-6=43%. these values indicate that glucose was degraded by the entner-doudoroff pathway alone. cells which grew in glucose-yeast extract-salts medium gave ... | 1969 | 5776525 |
| deoxyribonucleic acid base composition of isolates of rhizobium japonicum. | 1969 | 5786782 | |
| effect of azotobacter chroococcum and tryptophan on the inoculation of soybean (glycine max (l.) merril) with rhizobium japonicum. | 1969 | 5271557 | |
| transformation in rhizobium japonicum. | 1969 | 5365772 | |
| the regulation of some enzymes involved in ammonia assimilation by rhizobium japonicum. | 1969 | 5370296 | |
| competition between two somatic serotypes of rhizobium japonicum used as double-strain inocula in varying proportions. | 1969 | 5394016 | |
| electron transport systems of rhizobium japonicum. i. haemoprotein p-450, other co-reactive pigments, cytochromes and oxidases in bacteroids from n2-fixing root nodules. | 1969 | 4974059 | |
| electron transport systems of rhizobium japonicum. ii. rhizobium haemoglobin, cytochromes and oxidases in free-living (cultured) cells. | 1969 | 4974060 | |
| serological analysis of eleven strains of rhizobium japonicum. | 1969 | 4977516 | |
| transformation of infectivity in rhizobium japonicum. | 1970 | 4994104 | |
| effect of biotin on fatty acids and phospholipids of biotin-sensitive strains of rhizobium japonicum. | the effect of biotin on fatty acids and intact lipids was studied by comparing a biotin-requiring, a biotin-inhibited, and a biotin-indifferent strain of rhizobium japonicum. these organisms were grown in a defined medium with added levels of 0, 0.3, and 0.5 mug of biotin per liter, and were analyzed for fatty acids and lipid components. myristic, palmitic, and octadecenoic acids were found to be the major fatty acids in these strains. the indifferent strain also contained large amounts of c(19) ... | 1970 | 5437727 |
| gluconate catabolism in rhizobium japonicum. | gluconate catabolism in rhizobium japonicum atcc 10324 was investigated by the radiorespirometric method and by assaying for key enzymes of the major energy-yielding pathways. specifically labeled gluconate gave the following results for growing cells, with values expressed as per cent (14)co(2) evolution: c-1 = 93%, c-2 = 57%, c-3 = 30%, c-4 = 70%, c-6 = 39%. the preferential release of (14)co(2) from c-1 and c-4 indicate that gluconate is degraded primarily by the entner-doudoroff pathway but ... | 1970 | 5438044 |
| purification of aspartate aminotransferases from soya-bean root nodules and rhizobium japonicum. | 1970 | 5500327 | |
| antigenic analysis of rhizobium japonicum by immunodiffusion. | immunodiffusion reactions were studied with seven strains of rhizobium japonicum and three strains of the cowpea miscellany by using antisera against eight of the strains. most strains yielded only weak precipitin bands when untreated cell suspensions were used as antigens in the diffusions. ultrasonic disruption or heat treatment of the cells led to stronger bands, and immersion in boiling water for 20 min was used as the standard procedure for preparing these bacteria for immunodiffusion analy ... | 1971 | 4998353 |
| the biosynthesis of aspartic acid, glutamic acid, and alanine in rhizobium japonicum. | 1971 | 5087891 | |
| the phospholipid composition of rhizobium japonicum. | 1971 | 4323346 | |
| electron paramagnetic resonance and temperature dependent spin state studies of ferric cytochrome p-450 from rhizobium japonicum. | 1972 | 4339738 | |
| anaerobic-nitrate, symbiotic and aerobic growth of rhizobium japonicum: effects on cytochrome p 450 , other haemoproteins, nitrate and nitrite reductases. | 1972 | 4341774 | |
| deoxyribonucleate binding and transformation in rhizobium jpaonicum. | rhizobium japonicum, capable of binding high-molecular-weight donor (32)p-labeled deoxyribonucleic acid (dna) during late log phase in a competence medium, was transformed for streptomycin resistance with a frequency of transformation ranging between 0.02 and 0.08%. eight to 10% of the homologous native (32)p-labeled input dna was bound irreversibly in a temperature-dependent manner. homologous denatured (32)p-labeled dna was incapable of binding to the recipient under similar conditions. cscl d ... | 1972 | 4538250 |
| compatibility of different fungicides with rhizobium japonicum. | 1972 | 4564552 | |
| interactions between an unusual aspartate aminotransferase from rhizobium japonicum and pyridoxal-5'-phosphate studied by affinity chromatography. | 1972 | 5085272 | |
| studies on cytokinin production by rhizobium. | cytokinin was released into the medium by cultures of both rhizobium japonicum and r. leguminosarum. calculations show that the amount of cytokinin released during the logarithmic phase of growth by r. japonicum would be sufficient to initiate the cortical cell divisions necessary to form a root nodule. the substance released by r. japonicum was identified as a zeatin-like compound on the basis of paper chromatography in four solvent systems. two solvents clearly separated the rhizobial product ... | 1972 | 16657888 |
| isolation from rhizobium for factors which transfer electrons to soybean nitrogenase. | rhizobium japonicum grown anaerobically with nitrate produced several factors capable of transferring electrons from spinach chloroplasts lacking photosystem ii to soybean root nodule nitrogenase. the same organism grown aerobically produced no factors with detectable activity. it is concluded that the bacterium is potentially capable of producing these proteins in the low oxygen conditions of the leguminous root nodule. | 1973 | 16658274 |
| transmissible resistance to penicillin g, neomycin, and chloramphenicol in rhizobium japonicum. | the genetic basis for resistance to a number of antibiotics was examined in rhizobium japonicum. resistance to penicillin g, neomycin, and chloramphenicol appears to be mediated by an extrachromosomal element similar to that found in the enterobacteriaceae. resistance to these antibiotics was eliminated from cells by treatment with acridine orange, and resistance to all three antibiotics could be transferred en bloc to agrobacterium tumefaciens under conditions excluding transformation or transd ... | 1973 | 4491197 |
| competition between inoculum strains of rhizobium japonicum in the process of soybean nodulation during three planting periods. | 1973 | 4201820 | |
| relationship between soybean cultivars and rhizobium japonicum serotypes with single- and multi-strain inoculants. i. greenhouse pot experiments. | 1973 | 4205244 | |
| a simple medium for quick growth of rhizobium japonicum. | 1973 | 4580100 | |
| analysis of the intracellular amino acid pool and proteins from whole cells of rhizobium japonicum. | 1973 | 4583873 | |
| survival of rhizobium japonicum in various carriers. | 1973 | 4801277 | |
| relationship between soybean cultivars and rhizobium japonicum serotypes with single- and multi-strain inoculants. ii. field experiments. | 1973 | 4801803 | |
| distribution of the isopropylmalate pathway to leucine among diverse bacteria. | alpha-isopropylmalate synthase and beta-isopropylmalate dehydrogenase activities were detected in extracts of the following organisms: chromatium d, rhodopseudomonas spheroides, hydrogenomonas h16, pseudomonas aeruginosa, pseudomonas fluorescens, vibrio extorquens, rhizobium japonicum, alcaligenes viscolactis, escherichia coli b, proteus vulgaris, aerobacter aerogenes, salmonella typhimurium, micrococcus sp., micrococcus lysodeikticus, bacillus polymyxa, bacillus subtilis, and nocardia opaca. th ... | 1974 | 4829932 |
| l-arabinose metabolism in rhizobium japonicum. | l-arabinose was metabolized through an oxidative pathway by extracts of a strain of rhizobium japonicum. the findings showed that l-arabinose is converted into 2-keto-3-deoxy-l-arabonate, which is cleaved into glycoaldehyde and pyruvate. | 1974 | 4407017 |
| streptomycin resistance in rhizobium japonicum. | 1974 | 4441216 | |
| transformation of effectiveness in rhizobium japonicum. | 1974 | 4477931 | |
| lectins: a possible basis for specificity in the rhizobium--legume root nodule symbiosis. | soybean lectin labeled with fluorescein isothiocyanate combined specifically with all but 3 of 25 strains of the soybean-nodulating bacterium rhizobium japonicum. the lectin did not bind to any of 23 other strains representative of rhizobia that do not nodulate soybeans. the evidence suggests that an interaction between legume lectins and rhizobium cells may account for the specificity expressed between rhizobia and host plant in the initiation of the nitrogen-fixing symbiosis. | 1974 | 17812054 |
| pristionchus lheritieri as a carrier of rhizobium japonicum. | 1974 | 19308114 | |
| comparison of dna polymerase of rhizobium meliloti and alfalfa bacteroids. | dna dependent-dna polymerase activity was established and partially purified from extracts of cultured rhizobium meliloti, f-28, and nodule bacteroids (r. meliloti, f-28) of alfalfa plants (medicago sativa). polymerase activity in the partially purified fractions showed characteristic dependence on mg(2+), dna, and a full complement of deoxyribonucleoside triphosphates. dnase activity, preference of "activated" double strand dna, and inhibition by p-chloromercuribenzoate and mncl(2) were respons ... | 1975 | 16659337 |
| fructose 1,6-bisphosphate aldolase activity of rhizobium species. | fdp aldolase was found to be present in the cell-free extracts of rhizobium leguminosarum, rhizobium phaseoli, rhizobium trifolii, rhizobium meliloti, rhizobium lupini, rhizobium japonicum and rhizobium species from arachis hypogaea and sesbania cannabina. the enzyme in 3 representative species has optimal activity at ph 8.4 in 0.2m veronal buffer. the enzyme activity was completely lost by treatment at 60 degrees c for 15 min. the km values were in the range from 2.38 to 4.55 x 10(-6)m fdp. met ... | 1975 | 283 |
| [proceedings: plasmids in rhizobium japonicum]. | 1975 | 50812 | |
| nitrogen fixing activity in rhizobium japonicum separated from plant cell cultures. | induced by soy bean tissue cultures in socalled "tissue chambers", rhizobium japonicum str. 61-a-96 developed nitrogenase activity separated from the plant cells. the activity proceded for 48 h with a rate of 1 x 10(-8) nmol c2h4h-1 cell-1, which is about 6% of the activity measured for bacteroids from rhizobium japonicum in nodules of glycine max. | 1975 | 130001 |
| physical evidence of a plasmid in rhizobium japonicum. | 1975 | 1140246 | |
| acetylene reduction by transfilter suspension cultures of rhizobium japonicum. | 1975 | 1169944 | |
| involvement of oxyleghaemoglobin and cytochrome p-450 in an efficient oxidative phosphorylation pathway which supports nitrogen fixation in rhizobium. | cellular atp level, atp/adp ratio and nitrogenase activity rise when oxyleghaemoglobin is added to respiring suspensions of rhizobium japonicum bacteroids from soybean root nodules. increased gaseous o2 tension is much less efficient than oxyleghaemoglobin in stimulation of bacteroid atp production. studies with the inhibitor carbonyl cyanide m-chlorophenylhydrazone show this atp to be generated as a consequence of oxidative phosphorylation. n-phenylimidazole, a specific cytochrome p-450 inhibit ... | 1975 | 1169973 |
| nitrate reductase from bacteroides of rhizobium japonicum: enzyme characteristics and possible interaction with nitrogen fixation. | the soluble nitrate reductase of rhizobium japonicum bacteroids has been purified and its properties compared to those of aerobically grown cells. the enzymes from both sources are similar with molecular weights of about 70 000 suggesting no close relationship with the molybdo-protein component of nitrogenase. nitrite, the product of nitrate reductase, strongly inhibited the nitrogenase activity from bacteroids, at concentrations less than 100 mum. thus, an interference in the rate of nitrogen f ... | 1975 | 1170894 |
| role of pectic and cellulolytic enzymes in the invasion of the soybean by rhizobium japonicum. | past workers have suggested pectic enzyme involvement in the invasion of legumes by rhizobium. however, no role for pectic acid, pectin, or methyl cellulose depolymerase enzymes in the invasion of r. japonicum was suggested by the current study. seedling inoculation with infective bacteria did not result in increased enzyme activity. rhizobium japonicum cell-free culture extracts and 3-indoleacetic acid did not affect the activation, induction, or binding of these enzymes. | 1975 | 1172457 |
| effect on microorganisms of volatile compounds released from germinating seeds. | volatile compounds evolved from germinating seeds of slash pine, bean, cabbage, corn, cucumber, and pea were evaluated for their ability to support growth of microorganisms in liquid mineral salts media lacking a carbon source. growth of eight bacteria was measured turbidimetrically and of six fungi as dry weight of mycelium. volatiles caused increased growth of pseudomonas fluorescens, bacillus cereus, erwinia carotovora, agrobacterium tumefaciens, a. radiobacter, rhizobium japonicum, mucor muc ... | 1975 | 1201509 |
| leghaemoglobin and the supply of o2 to nitrogen-fixing root nodule bacteroids: presence of two oxidase systems and atp production at low free o2 concentration. | studies of rates of consumption of dissolved o2 by suspensions of bacteroids (rhizobium japonicum, strain cb1809) from soybean root nodules showed the presence of two different terminal oxidase systems. a high-affinity system, sensitive to inhibition by n-phenylimidazole and by carbon monoxide, was most active when the dissolved o2 was between 0-01 and 0-1 mum. at 1 mum-o2 or higher, this oxidase system had little activity and o2 was consumed largely by a low-affinity system insensitive to these ... | 1975 | 1239489 |
| immunological evidence for the capability of free-living rhizobium japonicum to synthesize a portion of a nitrogenase component. | immunodiffusion tests conducted under aerobic conditions demonstrated that cross-reactive material to antiserum prepared against the mo-fe protein component of nitrogenase from soybean nodule bacteroids was detectable in extracts of free-living rhizobium japonicum cells cultured in a standard medium under: aerobic conditions; aerobic conditions with nitrate; aerobic conditions with ammonia; anaerobic conditions with nitrate; and anaerobic conditions with nitrate and ammonia. the most intense pre ... | 1975 | 803382 |
| adsorption and selection of rhizobia with ion-exchange papers. | ion exchange papers were used to study the adsorption of 32p-labelled rhizobia on defined surfaces. two strains of rhizobium japonicum and one each of r. leguminosarum and r. lupini were compared with escherichia coli and bacillus subtilis. the ratio of adsorption to strong and to weak acid papers/strong and weak basic papers was consistantly higher for all rhizobial strains compared to the other bacteria. the process of desorption by increasing the ion-concentration causes about 35% desorption ... | 1975 | 242293 |
| oxygen requirement for acetylene reduction by pure cultures of rhizobia. | the oxygen and nutritional requirements for acetylene reduction by rhizobium japonicum and rhizobium sp. in liquid culture are described. the optimal oxygen concentration was about 0.1% in the gas phase, which is lower than that of any other known aerobic nitrogen-fixing microorganism. these organisms are also unique in that nitrogenase synthesis is not repressed in the presence of ammonium chloride under certain cultural conditions, in contrast to other wild-type bacteria. | 1976 | 931945 |
| ineffective and non-nodulating mutant strains of rhizobium japonicum. | mutant strains of rhizobium japonicum that were unable to allow the corsoy cultivar of soybean to reduce acetylene or fix n2 were isolated. these strains grow as well as the wild type in a variety of media. mutant strains sm1 and sm2 did not form nodules on the host plant; however, they reduced acetylene in the nonsymbiotic assay. strains sm3 and sm4 produced nodules that did not have the characteristic pink pigment caused by leghemoglobin. the nodules formed by these strains also were small. on ... | 1976 | 986388 |
| rhizobium japonicum derivatives differing in nitrogen-fixing efficiency and carbohydrate utilization. | four derivatives of rhizobium japonicum 110 were isolated on the basis of morphologically different colonies formed on yeast extract-mannitol-hm salts medium. all are able to nodulate lee soybeans. the bacteria-plant associations formed by each clone have measurable acetylene-reducing activity, but those formed by two of these clones (designated l1-110 and l2-110) are 5- to 10-fold less efficient than those formed by the others (designated i-110 and s-110). these derivatives were not detectable ... | 1976 | 988784 |
| nitrate reductase from anaerobically grown rhizobium japonicum. | the activity of nitrate reductase in rhizobium japonicum is controlled by oxygen tension, and not by nitrate. the enzyme from r. japonicum grown anaerobically in the presence of nitrate resembles that from bacteroids in having a molecular weight of about 69000 daltons; the enzyme from aerobically grown cells ahs a molecular weight of about 170000 daltons. both types of enzyme have similar km values, but differ in their sensitivity to kcn. | 1976 | 993777 |
| discrimination of rhizobium japonicum, rhizobium lupini, rhizobium trifolii, rhizobium leguminosarum and of bacteroids by uptake of 2-ketoglutaric acid, glutamic acid and phosphate. | rhizobium strains (one each of rh. japonicum, rh. lupini, rh. leguminosarum) take up 2-ketoglutaric acid in general much faster and from lower concentrations in the medium than strains of escherichia coli, bacillus subtilis and chromobacterium violaceum. a strain of enterobacter aerogenes, however, is more similar to some rhizobium strains. the same strains of rhizobium take up also phosphate much faster and from lower concentrations than the other bacteria tested. 4 strains of rh. lupini proved ... | 1976 | 818969 |
| glycerol metabolism in rhizobium. | four strains of rhizobium japonicum and one strain of r. trifolii were grown on glycerol and found to contain a soluble atp-glycerol kinase and a particulate glycerolphosphate dehydrogenase. both enzymes are induced by glycerol. the presence of nad+-or nadp+-glycerol dehydrogenase was not detected in any of the strains. no significant differences were found in the glycerol metabolic pathway between fast-and slow-growing rhizobia. | 1976 | 1260522 |
| genetic mapping of leucine and isoleucine-valine loci in rhizobium japonicum. | leucine and isoleucine-valine loci have been mapped in rhizobium japonicum. transformation analysis suggests a common pathway for isoleucine-valine biosynthesis. three-point reciprocal crosses indicated that all the leucine loci are not genetically linked. | 1976 | 1262320 |
| p-450 hemeproteins of rhizobium japonicum. purification by affinity chromatography and relationship to p-450cam and p-450lm-2. | 1976 | 1267795 | |
| immunofluorescent polar tips of rhizobium japonicum: possible site of attachment or lectin binding. | rhizobium japonicum usda 31 demonstrated marked polarity by binding homologous fluorescent antibody (fa) heavily on one end of the cell. fa prepared against r. japonicum strains 110 and 138, and against r. trifolii ta1 cross-reacted with strain 31 only in the polar tip region. no polar immunofluorescing tips could be seen with fa against two other strains of r. japonicum or with those against several unrelated microorganisms. common antigens localized only in a polar region were seen in many rhi ... | 1976 | 56334 |
| effect of lindane on radio-carbon (14c) incorporation by rhizobium japonicum. | experiments conducted in vitro with three levels (1, 2, and 5 ppm active ingredient) of the insecticide lindane (gamma-bhc) showed no effect on the growth of rhizobium japonicum, but altered the incorporation of radio-carbon (14c-glucose) into the different constituents of the growing cells. while all the three levels of the insecticide significantly depressed the incorporation of radiocarbon in the alcohol-extractable fraction, with no effect on the alcohol-ether soluble fraction of the cells, ... | 1976 | 65064 |
| the purification and properties of the glutamine synthetase from the cytosol of soya-bean root nodules. | the major portion of glutamine synthetase activity in root nodules of soya-bean plants is associated with the cytosol rather than with rhizobium japonicum bacteroids. glutamine synthetase accounts for about 2% of the total soluble protein in nodule cytosol. glutamine synthetase from nodule cytosol has been purified by a procedure involving fractionation with protamine sulphate, ammonium sulphate and polypropylene glycol, chromatography on deae-bio-gel a and bio-gel a-5m and affinity chromatograp ... | 1976 | 8035 |
| regulation of nitrogen fixation by rhizobia. export of fixed n2 as nh+4. | the metabolic fate of gaseous nitrogen (15n2) fixed by free-living cultures of rhizobia (root nodule bacteria) induced for their n2-fixation system was followed. a majority of the fixed 15n2 was found to be exported into the cell supernatant. for example, as much as 94% of the 15n2 fixed by rhizobium japonicum (soybean symbiont) was recovered as 15nh+4 from the cell supernatant following alkaline diffusion. several species of root nodule bacteria also exported large quantities of nh+4 from l-his ... | 1976 | 8100 |
| the molybdenum--iron protein of klebsiella pneumoniae nitrogenase. evidence for non-identical subunits from peptide 'mapping'. | the molybdenum- and iron-containing protein components of nitrogenase purified from klebsiella pneumoniae, azotobacter vinelandii, azotobacter chroococcum and rhizobium japonicum bacteroids all gave either one or two protein-staining bands after sodium dodecyl sulphate/polyacrylamide-gel electrophoresis, depending on the commercial brand of sodium dodecyl sulphate used. the single band obtained with k. pneumoniae mo-fe protein when some commercial brands of sodium dodecyl sulphate were used in t ... | 1976 | 779772 |
| relation between glutamine synthetase and nitrogenase activities in the symbiotic association between rhizobium japonicum and glycine max. | the activity and extent of adenylylation of glutamine synthetase was examined in both free-living and bacteroid forms of rhizobium japonicum in the presence of excess ammonia. ammonia caused an apparent repression of glutamine synthetase in free-living r. japonicum and adenylylation of the enzyme was also increased. in contrast, neither the activity nor the extent of adenylylation of the bacteroid enzyme was consistently affected by ammonium treatment of bacteroid suspensions. similar results we ... | 1976 | 16659522 |
| synergetic cultures of glycine max root cells and rhizobia separated by membrane filters. | when suspension cultures of actively growing soybean (glycine max l.) root cells were separated by two or three membrane filters from suspension cultures of the bacteria, a synergetic (cooperative) activation of nitrogenase was observed in the rhizobium japonicum used in the bacterial side. either plant cells or plant cell-conditioned medium was needed for this activation to take place. both acetylene reduction and hydrogen evolution by the activated r. japonicum persisted for several days after ... | 1976 | 16659545 |
| effect of protein additives on acetylene reduction (nitrogen fixation) by rhizobium in the presence and absence of soybean cells. | the effect of protein additives on acetylene reduction (n(2) fixation) by rhizobium associated with soybean cells (glycine max [l.] merr.) in vitro was studied. acetylene reduction was promoted on the basal medium supplemented with 1.4 mg of n/ml supplied as aqueous extracts of hexane-extracted soybean, red kidney beans (phaseolus vulgaris l.), or peas (pisum sativum l.). commercial samples of alpha-casein, or bovine serum albumin also promoted acetylene reduction at a concentration of 1.4 mg of ... | 1976 | 16659592 |
| root hair cell enhancement in tissue cultures from soybean roots: a useful model system: in vitro rhizobium symbiosis. | a technique for obtaining large numbers of root hair cells in cell cultures from soybeans is described. the cells were grown on agar containing the prairie regional laboratory b5 (prl-b5) medium for periods longer than 60 days. mixed populations of cultured root hair cells and cortical cells were used to study the in vitro association between soybean cells and rhizobium japonicum. the advantages of these types of root cell cultures in studies of symbiosis are discussed. | 1977 | 16659797 |
| heme synthesis in soybean root nodules: i. on the role of bacteroid delta-aminolevulinic acid synthase and delta-aminolevulinic acid dehydrase in the synthesis of the heme of leghemoglobin. | during nodulation of soybean (glycine max) by rhizobium japonicum, variations in the activities of two enzymes of heme biosynthesis, delta-aminolevulinic acid synthase (alas) and delta-aminolevulinic acid dehydrase (alad) are described. delta-aminolevulinic acid synthase activity is found in the bacteroid fraction of nodules, but is not detected in the plant fraction. bacteroid alas activity parallels heme accumulation during nodule development. delta-aminolevulinic acid dehydrase activity is fo ... | 1977 | 16660108 |
| role of lectins in plant-microorganism interactions: i. binding of soybean lectin to rhizobia. | highly purified soybean lectin (sbl) was labeled with fluorescein isothiocyanate (fitc-sbl) or tritium ((3)h-sbl) and repurified by affinity chromatography. fitc-sbl was found to bind to living cells of 15 of the 22 rhizobium japonicum strains tested. the lectin did not bind to cells of the other seven r. japonicum strains, or to cells of any of the nine rhizobium strains tested which do not nodulate soybean. the binding of the lectin to the sbl-positive strains of r. japonicum was shown to be s ... | 1977 | 16660121 |
| 6-phospho-d-gluconate:nad+ 2-oxidoreductase (decarboxylating) from slow-growing rhizobia. | 6-phospho-d-gluconate:nad+ 2-oxidoreductase (decarboxylating) (nad+-6pgd) was detected in several slow-growing strains of rhizobia, and no activity involving nadp+ was found in the same extracts. by contrast, fast-growing strains of rhizobia had nadp+-6pgd activity; most of them also had nad+-6pgd activity. nad+-6pgd was partially purified from the slow-growing strain rhizobium japonicum 5006. the reaction was shown to be an oxidative decarboxylation. | 1977 | 16867 |
| effects of pesticide seed treatments on rhizobium japonicum and its symbiotic relationship with soybean. | seventeen rhizobium japonicum cultures isolated from soybean nodules induced formation of nodules on taproots of soybean plants. all isolates reduced acetylene to ethylene to different extents in vitro. paper disc assay indicated that two insecticides, lindane (gamma-1,2,3,4,5,6-hexachlorocyclohexane), chlorpyrifos (o,o-diethyl o-3,5,6-trichloro-2-pyridyl phosphorothioate), and a fungicide, thiram (tetramethylthiuram disulphide) individually or in combination caused significant inhibition of the ... | 1977 | 70252 |
| flow-microfluorometric analysis of escherichia coli, rhizobium meliloti, and rhizobium japonicum at different stages of the growth cycle. | the applicability of flow-microfluorometry (fmf) to the study of bacterial samples was investigated on cultures of rhizobium meliloti, rhizobium japonicum, and escherichia coli using fluorescent and light-scattering signals. this technique which analyzes individual bacterial cells in a population was used to monitor the relative change in nucleic acid content and cell size during the growth cycle of the three microorganisms which were known to have different growth rates. early log-phase e. coli ... | 1977 | 332295 |
| introduction of potential sex factors into rhizobium japonicum. | 1977 | 411474 | |
| antibiotic resistance in rhizobium japonicum: implications for safety and interpretation of nif transfer from rhizobium. | 1977 | 411475 | |
| ultrastructure of soybean nodules. i: release of rhizobia from the infection thread. | root nodules on soybeans (var. clark 63) were examined by transmission electron microscopy 10-12 days after seed inoculation and planting. the cell infection process appeared identical in both effective nodules, induced by rhizobium japonicum strain 138 (usda) and in ineffective nodules, induced by strain 8-0 (iowa). electron micrographs are presented which suggest that rhizobia are freed from the infection thread by disintegration of the thread wall and compartmentalization of the distintegrat ... | 1977 | 559533 |
| glucose catabolism in two derivatives of a rhizobium japonicum strain differing in nitrogen-fixing efficiency. | radiorespirometric and enzymatic analyses reveal that glucose-grown cells of rhizobium japonicum isolates i-110 and l1-110, both derivatives of r. japonicum strain 3i1b110, possess an active tricarboxylic acid cycle and metabolize glucose by simultaneous operation of the embden-meyerhof-parnas and entner-doudoroff pathways. the hexose cycle may play a minor role in the dissimilation of glucose. failure to detect the nicotinamide adenine dinucleotide phosphate-dependent decarboxylating 6-phosphog ... | 1977 | 559670 |
| comparison of colony morphology, salt tolerance, and effectiveness in rhizobium japonicum. | four strains of rhizobium japonicum, two of which produce slimy and non-slimy colony types and two others which produce large and small colony types, were isolated and cloned. all were infective and nodulated lee soybean host plants. each colony type was characterized as to its salt sensitivity to na+ and k+ ions, relative level of symbiotic nitrogen fixation, and relative level of free-living nitrogen fixation. growth studies performed in the presence of salts demonstrated that the non-slimy or ... | 1977 | 561643 |
| grouping of rhizobial strains--a method based on symbiotic characteristics. | twenty strains of rhizobium japonicum and non-inoculated control were used to study seven symbiotic characteristics with two varieties of soybean (glycine max). the strains were then grouped on the basis of these symbiotic characteristics, using mahalanobis' d2 statistical method. eight groups were formed in which two strains stood distinctly aloof, indicating thereby the exceptional nature of these strains over others in their symbiotic behaviour. this method is suggested for selecting exceptio ... | 1977 | 565116 |
| stepwise selection of efficient rhizobial cultures through cultural characteristics. | nodulation and shoot nitrogen of two varieties of soybean (glycine max) were studied with twenty strains of rhizobium japonicum. a number of cultural characteristics of the strains in isolation to the symbiotic system were also studied. a stepwise selection method was employed for detecting efficient cultures through the cultural characteristics which showed association with the steps in the symbiotic system. nodulation of one variety was found to be associated with the dehydrogenase activity an ... | 1977 | 565117 |
| some features of mannitol metabolism in rhizobium japonicum. | 1977 | 833572 | |
| polarity in the exponential-phase rhizobium japonicum cell. | highly distinctive aspects of the exponentail-phase rhizobium japonicum cell were disclosed by means of thin sections, freeze etching, fluorescent antibodies, and ruthenium red staining. polarity was expressed in the form of reserve polymer distribution near one end of the cell and as cytoplasmic localization near the opposite end. in addition, exocellular polysaccharide (eps) accumulated preferentially around the cytoplasmic end, and the feature described previously as an "immunofluorescent pol ... | 1977 | 907920 |
| purification and characterisation of d-amino acid aminotransferase from rhizobium japonicum. | rhizobium japonicum has d-amino acid aminotransferase and alanine racemase activities. the d-amino-acid aminotransferase has been partially purified and characterized. this enzyme has a broad specificity and is very active with d-alpha-aminobutyrate and d-aspartate as well as d-alanine and d-glutamate. the stereospecificity of the enzyme for d-amino acids was apparently absolute with respect to product inhibition, pyridoxamine formation as well as catalytic activity. the apparent molecular weigh ... | 1978 | 620041 |