Publications

acid-base titration across the plasma membrane of micrococcus denitrificans: factors affecting the effective proton conductance and the respiratory rate.		1970	5005951
fluorescent probe environment and the structural and charge changes in energy coupling of mitochondrial membranes.	the use of fluorescent probes to give continuous readouts of the structural states of mitochondrial membranes during energy coupling seems a logical extension of their use in the study of protein structural changes. a clear correlation of the probes' fluorescence characteristics with the acquisition of energy coupling can be demonstrated in fragmented and natural membrane using 1-anilinonaphthalene-8-sulfonate (ans) and ethidium bromide respectively. the present contribution attempts to bring to ...	1970	5289010
respiration-driven proton translocation in micrococcus denitrificans.		1971	5135306
release of respiratory control in particles from micrococcus denitrificans by ion-translocating antibiotics.		1971	5139216
[the influence of x-irradiation on growth and respiration of micrococcus denitrificans beijerinck].		1971	5172514
branched electron-transport systems in bacteria.		1971	5005815
phospholipid composition and metabolism of micrococcus denitrificans.	the phospholipid composition of micrococcus denitrificans was unusual in that phosphatidyl choline (pc) was a major phospholipid (30.9%). other phospholipids were phosphatidyl glycerol (pg, 52.4%), phosphatidyl ethanolamine (pe, 5.8%), an unknown phospholipid (5.3%), cardiolipin (cl, 3.2%), phosphatidyl dimethylethanolamine (pdme, 0.9%), phosphatidyl monomethylethanolamine (pmme, 0.6%), phosphatidyl serine (ps, 0.5%), and phosphatidic acid (0.4%). kinetics of (32)p incorporation suggested that p ...	1972	4640503
the bacterial nitrate reductases: epr studies on nitrate reductase a from micrococcus denitrificans.		1972	4335844
preliminary x-ray studies of a cytochrome c from micrococcus dentrificans.		1972	4346098
the influence of oxygen and nitrate on the formation of the cytochrome pigments of the aerobic and anaerobic respiratory chain of micrococcus denitrificans.		1972	5042842
5-aminolaevulinate synthetase of micrococcus denitrificans.		1972	5085583
recurrence of the cytochrome fold in a nitrate-respiring bacterium.		1973	4355597
interaction of cytochrome oxidases aa3 and d with nitrite.		1973	4357109
structure of micrococcus denitrificans and m. halodenitrificans revealed by freeze-etching.		1973	4717955
aminolaevulinate synthetase of micrococcus denitrificans. purification and properties of the enzyme, and the effect of growth conditions on the enzyme activity in cells.	1. 5-aminolaevulinate synthetase was detected in extracts of the non-photosynthetic bacterium micrococcus denitrificans. 2. activity is high in cells grown anaerobically in a defined nitrate medium, but is low in cells grown in an iron-deficient medium, and in cells grown aerobically. 3. aminolaevulinate synthetase was purified extensively; it has a molecular weight of about 68000; apparent k(m) values for glycine, succinyl-coa and pyridoxal phosphate are 12mm, 10mum and 11mum respectively; 2mum ...	1973	4722442
control of aminolaevulinate synthetase in micrococcus denitrificans.		1973	4791044
mechanisms of active transport in isolated bacterial membrane vesicles. 8. valinomycin-induced rubidium transport.		1973	4573982
[bacterial lipids].		1974	4156687
selective and reversible inhibition of the atpase of micrococcus denitrificans by 7-chloro-4-nitrobenzo-2-oxa-1,3 diazole.		1974	4278263
reduction and separation of nitrate and nitrite by liquid membrane-encapsulated enzymes.		1974	4854059
relationship between amino acid transport and electron transport by membrane vesicles of micrococcus denitrificans.		1974	4441098
effect of the systemic fungicide carboxin on electron transport function in membranes of micrococcus denitrificans.	the systemic fungicide carboxin (5,6-dihydro-2-methyl-1,4-oxathiin-3-carboxanilide) inhibited oxidation of succinate by membranes prepared from micrococcus denitrificans, the k(i) being 16 mum. oxycarboxin (5,6-dihydro-2-methyl-1,4-oxathiin-3-carboxanilide-4,4-dioxide), f831 (5,6-dihydro-2-methyl-1,4-oxathiin-3-carboxanilide-4-oxide), and another succinate oxidase inhibitor, 4,4,4-trifluoro-1-(2-thienyl)-1,3-butanedione (ttb) were less effective inhibitors of succinate oxidation by membranes of ...	1974	15825307
nitrate and nitrite reduction by liquid membrane-encapsulated whole cells.		1975	21712
paracoccus denitrificans: a present-day bacterium resembling the hypothetical free-living ancestor of the mitochondrion.		1975	183302
the reversibility of active sulphate transport in membrane vesicles of paracoccus denitrificans.	an uncoupler-sensitive active transport of sulphate into membrane vesicles prepared from the plasma membrane of paracoccus denitrificans (previously micrococcus denitrificans) can be driven by respiration or by a trans-membrane ph gradient (alkaline inside) generated by the addition either of kcl ( in the presence of nigericin) or of nh4cl. valinomycin does not substitute for nigericin. respiration-driven transport is observed in right-side-out vesicles but not in inside-out vesicles, whereas tr ...	1975	1212205
the autotrophic growth of micrococcus denitrificans on methanol.	ribulose bisphosphate carboxylase is present at a high specific activity in extracts of methanol-grown microccus denitrificans. enzymic and physiological evidence indicates that, during growth on methanol, the ribulose bisphosphate cycle is the route of carbon assimilation.	1975	1212208
phosphate transport in membrane vesicles of paracoccus denitrificans.		1975	1225582
paracoccus denitrificans and the evolutionary origin of the mitochondrion.		1975	235742
the identification and biosynthesis of siderochromes formed by micrococcus denitrificans.	1. micrococcus denitrificans excretes three catechol-containing compounds, which can bind iron, when grown aerobically and anaerobically in media deficient in iron, and anaerobically in medium with a high concentration of ca2+. 2. one of these compounds was identified as 2,3-dihydroxybenzoic acid (compound i), and the other two were tentatively identified as n1n8-bis-(2,3-dihydroxybenzoyl)spermidine (compound ii) and 2-hydroxybenzoyl-n-l-threonyl-n4[n1n8-bis-(2,3-dihydroxybenzoyl)]spermidine (co ...	1975	238503
electron transport in aerobically grown paracoccus denitrificans: kinetic characterization of the membrane-bound cytochromes and the stoichiometry of respiration-driven proton translocation.		1976	1278392
the transport of d-lactate by membrane vesicles from paracoccus denitrificans.		1976	1278402
the cytochrome fold and the evolution of bacterial energy metabolism.		1976	176369
amino acid sequence of paracoccus denitrificans cytochrome c550.	the amino acid sequence of paracoccus (formerly micrococcus) denitrificans cytochrome c550 has been established by a combination of standard chemical techniques and interpretation of a 2.5 a resolution x-ray electron density map. peptides derived from a trypsin digest were chemically sequenced, and then ordered by fitting them to the density map. the amino acid compositions of chymotryptic peptides confirmed the x-ray map ordering the tryptic peptides. the amino acid sequence of this respiratory ...	1976	177408
oxidative phosphorylation in micrococcus denitrificans: calculation of the p/o ratio in growing cells.	p/o ratios were measured in membrane particles obtained from cells of micrococcus denitrificans, while growing on different carbon sources. the membrane particles obtained from cells growing actively on glucose, succinate, ethanol and propanol as the carbon and energy sources catalyzed oxidative phosphorylation and yielded respective p/o ratios of 1.4, 1.2, 0.8, and 0.5 with nadh, and 0.8, 0.6, 0.6, and 0.5 with succinate as the electron donors. not such a difference in p/o ratio is observed in ...	1976	179486
the structure of paracoccus denitrificans cytochrome c550.	the crystal structure of paracoccus (formerly micrococcus) denitrificans cytochrome c550 has been solved by x-ray diffraction to a resolution of 2.45 a. in both amino acid sequence and molecular structure it is evolutionarily homologous with mitochondrial cytochrome c from eukaryotes and photosynthetic cytochrome c2 from purple non-sulfur bacteria. all of these cytochromes c have the same basic folding pattern, with surface insertions of extra amino acids in c550. various strains of c2 have all, ...	1976	180013
pathway of thiamine pyrophosphate synthesis in micrococcus denitrificans.	the pathway of thiamine pyrophosphate (tpp) biosynthesis, which is formed either from exogeneously added thiamine or from the pyrimidine and thiazole moieties of thiamine, in micrococcus denitrificans was investigated. the following indirect evidence shows that thiamine pyrophosphokinase (ec 2.7.6.2) catalyzes the synthesis of tpp from thiamine: (i) [35s]thiamine incubated with cells of this microorganism was detected in the form of [35s]thiamine; (ii) thiamine gave a much faster rate of tpp syn ...	1976	181359
distribution and effect of the weak acids 5,5-dimethyl-2,4-oxazolidinedione (dmo) and 2,4-dinitrophenol (dnp) in membrane vesicles of micrococcus denitrificans.		1976	9905
the mitochondrial ribosomes of neurospora crassa. ii. comparison of the proteins from neurospora crassa mitochondrial ribosomes with ribosomal proteins from neurospora cytoplasm, from rat liver mitochondria and from bacteria.	1. it has been shown by datema et al. (datema, r., agsteribbe, e. and kroon, a.m. (1974) biochim. biophys. acta 335, 386--395) that neurospora mitochondria isolated in a mg2+-containing medium (or after homogenization of the mycelium in this medium and subsequent washing of the mitochondria in edta-containing medium) possess 80-s ribosomes; mitochondria homogenized and isolated in edta medium yield 73-s ribosomes. the ribosomal proteins of the subunits of 80-s and 73-s ribosomes were compared by ...	1976	133723
possible cross-reactivity of human anti-mitochondrial antibodies with membrane vesicles of paracoccus denitrificans.		1976	137140
[no stimulation of the growth of microorganisms by repair-substances (author's transl)].	by means of manometric technique the influence of x-ray-repairsubstances on the growth and respiration of micrococcus denitrificans, micrococcus radiodurans and saccharomyces cerevisiae was investigated. though these substances are stimulating the development of higher plants, there is no effect on growth and respiration of these microorganisms.	1976	798454
acetylene inhibition of nitrous oxide reduction by denitrifying bacteria.		1976	817722
[hydrogen and carboxide bacteria belonging to the microflora of degradation].	the following bacteria oxidizing hydrogen and/or carbon monoxide were shown by cultivating in the concentration gradient of acetate, lactate, and glucose, to belong to the microflora of dispersal: hydrogenomonas eutropha z-1, h. pantotropha z-11, pseudomonas carboxydoflava z-1107, achromobacter carboxydus z-1171, paracoccus denitrificans, and alcaligenes paradoxus.	1976	820943
metal accumulation by bacteria with particular reference to dissimilatory sulphate-reducing bacteria.	dissimilatory sulphate-reducing bacteria, genera desulfovibrio and desulfotomaculum, exhibit a superior ability, over assimilatory organisms, to extract three amounts of metals from culture media. this property does not appear to be solely a function of the presence of h2s. in media containing elevated amounts of fe, electron dense particles, provisionally identified as fes, are deposited within the cells of dissimilatory bacteria.	1976	824869
a new pathway for the biosynthesis of spermidine.		1976	1087258
aerobic and anaerobic bacterial respiration monitored by electrodes.	a technique is described by which both oxygen and nitrate (or nitrate or chlorate) levels were continuously monitored during bacterial respiration. paracoccus (micrococcus) denitrificans and escherichia coli oxidizing succinate rapidly ceased to reduce nitrate when oxygen was available, and equally rapidly commenced nitrate reduction when all the oxygen had been consumed. by contrast, membrane vesicles isolated from p. denitrificans reduced oxygen and nitrate simultaneously. the respiratory nitr ...	1977	319200
energy conservation during nitrate respiration in paracoccus denitrificans.	p/2e ratios were calculated from anaerobic chemostat cultures of paracoccus denitrificans with nitrogenous oxides as electron acceptor. p/2e ratios were calculated, using the ymaxatp values determined for aerobic cultures. when succinate was the carbon and energy source the average p/2e values of the sulphate- and succinate-limited cultures with nitrate as electron acceptor were 0.5 and 0.7, respectively, and of the nitrite-limited culture 0.9. with gluconate as carbon and energy source and aver ...	1977	843167
energy conservation during aerobic growth in paracoccus denitrificans.	paracoccus denitrificans was aerobically grown in chemostat culture with succinate or gluconate as carbon source. due to the presence of two phosphorylation sites in the respiratory chain and the absence of branching, theoretical p/o ratios of 1.71 and 1.82 were calculated for cells growing respectively with succinate and gluconate as carbon source. using these data, 95% confidence intervals for the p/o ratio were determined, via a mathematical model, at 0.91-1.15 and 1.00-1.37 for sulphate-limi ...	1977	843168
numerically dominant denitrifying bacteria from world soils.	nineteen soils, three freshwater lake sediments, and oxidized poultry manure were examined to determine the dominant denitrifier populations. the samples, most shown or expected to support active denitrification, were from eight countries and included rice paddy, temperate agricultural, rain forest, organic, and waste-treated soils. over 1,500 organisms that could grow anaerobically on nitrate agar were isolated. after purification, 146 denitrifiers were obtained, as verified by production of n( ...	1977	869539
localization and stability of hydrogenases from aerobic hydrogen bacteria.	alcaligenes eutrophus strains h 16, b 19, g 27 and n9a contained two different hydrogenases. one enzyme catalyzed the reduction of nad by hydrogen and was strictly localized in the soluble cell fraction. while the second enzyme was found to be particulate and unable to react with nad. all other tested strains, alcaligenes paradoxus sa 29, pseudomonas facilis, p. palleronii rh 2, pseudomonas sp. strain ga 3, paracoccus denitrificans, aquaspirillum autotrophicum sa 32, and corynebacterium autotrop ...	1977	871226
chemical transformation of 4-chloroaniline to a triazene in a bacterial culture medium.		1977	881504
primary structure of a high potential iron sulfur protein from a moderately halophilic denitrifying coccus.	the occurrence of a specific high potential iron-sulfur protein in a halophilic, denitrifying coccus provisionally assigned to the genus paracoccus has been confirmed through primary structure determination. this protein, known as "hipip", has been found previously only in photosynthetic bacteria. the sequence of this 71-residue protein is as similar to the hipeps from photosynthetic bacteria as they, in turn, are to one another (average number of identically matching residues is 38%). paracoccu ...	1977	914842
[a physiological and taxonomic study of paracoccus denitrificans (author's transl)].		1977	931260
metabolic regulation of pyruvate kinase isolated from autotrophically and heterotrophically grown paracoccus denitrificans.	pyruvate kinase (atp: pyruvate phosphotransferase (ec 2.7.1.40) was partially purified from both autotrophically and heterotrophically grown paracoccus denitrificans. the organism grown under heterotrophic conditions contains four times more pyruvate kinase than under autotrophic conditions. the enzyme isolated from both sources exhibited sigmoidal kinetics for both phosphoenolpyruvate (pep) and adp. the apparent km for adp and pep in the "autotrophic" enzyme were 0.63 mm adp and 0.25 mm pep. th ...	1977	931510
micromorphology of gram-negative hydrogen bacteria. i. cell morphology and flagellation.	the cell morphology, the arrangement and fine structure of flagella and the piliation of the following gram-negative aerobic hydrogen bacteria have been studied: alcaligenes eutrophus, alcaligenes paradoxus, alcaligenes ruhlandii, pseudomonas flava, pseudomonas pseudoflava, pseudomonas palleronii, pseudomonas facilis, aquaspirillum autotrophicum, paracoccus denitrificans, corynebacterium autotrophicum, and strains ma 2 and sa 35. the identity of the bacteria was examined by their substrate spect ...	1977	410385
tightly bound nucleotides of the energy-transducing atpase, and their role in oxidative phosphorylation. i. the paracoccus denitrificans system.	1. the coupling atpase of paracoccus denitrificans can be removed from the membrane by washing coupled membrane fragments at low salt concentrations. 2. this atpase resembles coupling atpases of mitochondria, chloroplasts and other bacteria. it is a negatively charged protein of molecular weight about 300,000. an inhibitor protein in bound tightly to the atpase in vivo, and can be destroyed by trypsin treatment. 3. atp and adp are found tightly bound to the coupling atpase of p. denitrificans, b ...	1977	139162
bacterial respiration.		1977	140652
some contemporary problems in electron-transport-linked adenosine triphosphate synthesis and related processes.		1977	143382
multiple low spin forms of the cytochrome c ferrihemochrome. epr spectra of various eukaryotic and prokaryotic cytochromes c.	1. despite the same methionine-sulfur:heme-iron:imidazole-nitrogen hemochrome structure observed by x-ray crystallography in four of the seven c-type eukaryotic and prokaryotic cytochromes examined, and the occurrence of the characteristic 695 nm absorption band correlated with the presence of a methionine-sulfur:heme-iron axial ligand in all seven proteins, they fall into two distinct classes on the basis of their epr and optical spectra. the horse, tuna, and bakers' yeast iso-1 cytochromes c ...	1977	13072
metabolic regulation of malic enzyme activity from paracoccus denitrificans by glyoxylate and acetylcoa.		1977	14633
sulphur metabolism in paracoccus denitrificans. purification, properties and regulation of serine transacetylase, o-acetylserine sulphydrylase and beta-cystathionase.	1. serine transacetylase, o-acetylserine sulphydrylase and beta-cystathionase were purified from paracoccus denitrificans strain 8944. 2. serin transacetylase was purified 150-fold. the enzyme has a ph optimum between 7.5 and 8.0, is specific for l-serine and is inhibited by sulphydryl-group reagents. the apparent km values for serine and acetyl-coa are 4.0 - 10(-4) and 1.0 - 10(-4) m, respectively. serine transacetylase is strongly inhibited by cysteine. 3. o-acetylserine sulphydrylase was puri ...	1977	14692
sulphur metabolism in paracoccus denitrificans. purification, properties and regulation of cysteinyl-and methionyl-trna synthetase.	cysteinyl- and methionyl-trna synthetases (ec 6.11.-) were purified 1200- and 1000-fold, respectively, from sonic extracts of paracoccus denitrificans strain 8944, and kinetics, substrate specificity and regulatory properties were determined using the atp-ppi exchange reaction. both enzymes had ph optima of approx. 8 and were inhibited by sulphydryl-group reagents. cysteinyl-trna synthetase catalysed l-selenocysteine- and alpha-aminobutyric acid-dependent atp-ppi exchange and methionyl-trna synt ...	1977	14693
metabolic regulation of the glucose-6-phosphate dehydrogenase from paracoccus denitrifcans grown on glucose/nitrate.	glucose-6-phosphate dehydrogenase (d-glucose-6-phosphate : nadp+ l-oxidoreductase ec 1.1.1.49) isolated from paracoccus denitrificans grown on glucose/nitrate exhibits both nad+-and nadp+- linked activities. both activities have a ph optimum of ph 9.6 (glycine/naoh buffer) and neither demonstrates a mg2+ requirement. kinetics for both nad(p)+ and glucose-6-phosphate were investigated. phosphoenolpyruvate inhibits both activities in a competitive manner with respect to glucose-6-phosphate. atp in ...	1977	15531
the bioenergetics of paracoccus denitrificans.		1977	20140
internally generated reduced nicotinamide adenine dinucleotide as a substrate for glycine transport by membrane vesicles of paracoccus denitrificans.	internally generated reduced nicotinamide adenine dinucleotide was the most efficient substrate for glycine transport by membrane vesicles of paracoccus denitrificans.	1977	187573
reduction of iron and synthesis of protoheme by spirillum itersonii and other organisms.	membranes from spirillum itersonii reduce ferric iron to ferrous iron with reduced nicotinamide adenine dinucleotide or succinate as a source of reductant. iron reduction was measured spectrophotometrically at 562 nm using ferrozine, which chelates ferrous iron specifically. reduced nicotinamide adenine dinucleotide or succinate was also effective as a source of iron. the effects of respiratory inhibitors suggested that reduction of iron occurs at one or more sites on the respiratory chain befor ...	1977	190208
specific thiamine monophosphate phosphohydrolase in micrococcus denitrificans.	a phosphohydrolase specific for thiamine monophosphate was isolated from micrococcus denitrificans, partially purified approximately 100-fold, and separated from acid phosphatase.	1977	192716
purification and properties of an acid phosphatase of micrococcus denitrificans distinct from thiamine phosphate phosphatase.	to determine whether the acid phosphatase in micrococcus denitrificans participates in hydrolysis of thiamine phosphate in the synthesis of thiamine pyrophosphate, acid phosphatase was purified 280-fold by conventional procedures, which removed thiamine phosphate phosphatase completely. studies showed that this acid phosphatase is a different protein from thiamine phosphate phosphatase and that it has no binding site for thiamine phosphate on its active site.	1977	197075
structure and function of cytochromes c.		1977	197877
micromorphology of gram-negative hydrogen bacteria. ii. cell envelope, membranes, and cytoplasmic inclusions.	the fine structure of the cell envelope, of membrane systems and of cytoplasmic inclusions of gram-negative aerobic hydrogen bacteria has been studied. the results have been tabulated, and three main groups could be recognized: group 1: alcaligenes eutrophus, a.paradoxus, a.ruhlandii, pseudomonas facilis, p.flava, p.pseudoflava, p.palleronii, and aquaspirillum autotrophicum; group 2: "corynebacterium" autotrophicum and strains ma 2 and sa 35; group 3: paracoccus denitrificans. special structur ...	1977	199126
the role of iron-sulfur center 2 in electron transport and energy conservation in the nadh-ubiquinone segment of the respiratory chain in paracoccus denitrificans.	1. the electron paramagnetic resonance spectra at 15 k of reduced membrane particles of paracoccus denitrificans exhibit resonance signals with g values, line shapes and temperature profile which are similar to the signals of the iron-sulfur centers observed in the nadh-ubiquinone segment of mitochondrial respiratory chains. these iron-sulfur centers are reducible with nadh, nadph as well as chemically with dithionite. 2. sulphate-limited growth of paracoccus denitrificans results in the loss of ...	1977	202453
the inhibitor-sensitivity of the plasma-membrane adenosine triphosphatase of paracoccus denitrificans: comparison with the mitochondrial adenosine triphosphatase [proceedings].		1977	72696
irradiation of micro-organisms with mono-energetic x-rays; biological consequences of the auger effect.	the radiation resonance effect reported previously for isolated biomolecules has now for the first time been observed in a cellular system. dried bacteria, micrococcus denitrificans, in which tdr in dna was partially substituted by budr, were subjected to mono-energetic x-rays of energies below or above the k-edge for br. subsequently, the colony-forming ability was assayed. for photon energy slightly above the k-edge, the lethality/rad was greater than that below the k-edge. this is interpreted ...	1978	309443
regulation of energy metabolism: evidence against a primary role of adenine mucleotide translocase.		1978	204515
growth yields and the efficiency of oxidative phosphorylation during autotrophic growth of paracoccus denitrificans on methanol and formate.		1978	211973
the protonmotive force in phosphorylating membrane vesicles from paracoccus denitrificans. magnitude, sites of generation and comparison with the phosphorylation potential.	1. the magnitude of the protonmotive force in phosphorylating membrane vesicles from paracoccus denitrificans was estimated. the membrane potential component was determined from the uptake of s(14)cn(-), and the transmembrane ph gradient component from the uptake of [(14)c]methylamine. in each case a flow-dialysis technique was used to monitor uptake. 2. with nadh as substrate, the membrane potential was about 145mv and the ph gradient was below 0.5 ph unit. the membrane potential was decreased ...	1978	212022
the variable cytochrome content of paracoccus denitrificans grown aerobically under different conditions.		1978	213309
purification of bacterial cytochrome c by isoelectric focusing.		1978	213681
on the current-voltage relationships of energy-transducing membranes: phosphorylating membrane vesicles from paracoccus denitrificans [proceedings].		1978	217778
transmembrane electrical and ph gradients of paracoccus denitrificans and their relationship to oxidative phosphorylation.		1978	24551
electron-transport chain and coupled oxidative phosphorylation in methanol-grown paracoccus denitrificans.		1978	29587
studies of relationship among terrestrial pseudomonas, alcaligenes, and enterobacteria by an immunological comparison of glutamine synthetase.	antibody to purified glutamine synthetase from escherichia coli was prepared and used for an immunological comparison of glutamine synthetases from species of salmonella, citrobacter, enterobacter, serratia, proteus, erwinia, aeromonas, pseudomonas, acinetobacter, xanthomonas, alcaligenes, and paracoccus. the results of ouchterlony double diffusion experiments and quantitative microcomplement fixation studies indicated that the amino acid sequence of this enzyme was highly conserved in different ...	1978	31146
respiration-dependent proton translocation and the transport of nitrate and nitrite in paracoccus denitrificans and other denitrifying bacteria.		1978	31172
the interactions of coupling atpases with nucleotides.		1978	147104
energy transduction in the mitochondrionlike bacterium paracoccus denitrificans during carbon- or sulphate-limited aerobic growth in continuous culture.	paracoccus denitrificans was grown in carbon-limited aerobic continuous culture (critical dilution rate (dc) = 0.48 h-1). the molar growth yield for carbon (succinate or malate) was constant at about 60 over a broad dilution range (growth rate) from 0.10 to 0.48 h-1. measurements of the stoichiometry of proton translocation associated with the oxidation of endogenous substrates yielded a ratio of protons ejected from the cell per atom of oxygen consumed(leads to h+:o) of 8.55 which decreased to ...	1978	36970
rapid oxygen-induced reduction of b-type cytochromes in paracoccus denitrificans.		1978	620789
ribulose bisphosphate carboxylase from methanol-grown paracoccus denitrificans.	paracoccus denitrificans grows on methanol as the sole source of energy and carbon, which it assimilates aerobically via the reductive pentose phosphate cycle. this gram-negative bacterium grew rapidly on 50 mm methanol (generation time, 7 h, 30 degrees c) in excellent yield (3 g of wet-packed cells per liter of culture). electron microscopic studies indicated that the late-log-phase cells were coccoid, having a thick envelope surrounding a layer of more diffuse electron-dense material and a rel ...	1978	659365
hydrogenase activity in paracoccus denitrificans. partial purification and interaction with the electron transport chain.		1978	667184
the preparation of tightly coupled membrane vesicles from paracoccus denitrificans.		1978	677462
dissimilatory nitrate reduction by liquid membrane encapsulated cell-free extracts and whole cells of micrococcus denitrificans.	the combination of sephadex g-15 and ion-exchange resin columns allows one-step desalting and separation of cellodextrins using water as the sole eluent. the column apparatus described in this paper has the potential of producing up to 3 g cellodextrins in one day. in addition, the columns described are stable and do not require repacking or regeneration after each run. hence the potential exists for scaling up this system for even greater production of cellodextrins if need be.	1978	708860
purification of (4fe-4s)1--2--ferredoxins (high-potential iron--sulfur proteins) from bacteria.		1978	713842
purification of electron-transfer components of nitrifying and denitrifying bacteria and assay of intermediates.		1978	713858
aerobic and anaerobic growth of paracoccus denitrificans on methanol.	1. the dye-linked methanol dehydrogenase from paracoccus denitrificans grown aerobically on methanol has been purified and its properties compared with similar enzymes from other bacteria. it was shown to be specific and to have high affinity for primary alcohols and formaldehyde as substrate, ammonia was the best activator and the enzyme could be linked to reduction of phenazine methosulphate. 2. paracoccus denitrificans could be grown anaerobically on methanol, using nitrate or nitrite as elec ...	1978	718372
effects induced by rotenone during aerobic growth of paracoccus denitrificans in continuous culture. changes in energy conservation and electron transport associated with nadh dehydrogenase.		1978	727852
induction by cyanide of cytochrome d in the plasma membrane of paracoccus denitrificans.		1979	437107
evidence from 31p nuclear magnetic resonance that polyphosphate synthesis is a slip reaction in paracoccus denitrificans [proceedings].		1979	437269
[relationship of hydrogen bacteria to carbon monoxide].	the purpose of this work was to study the ability of various cultures of hydrogen bacteria to grow in a gaseous mixture containing, apart from h2, o2 and co2, carbon monoxide at a concentration of 10% (v/v) and higher. in contrast to co-oxidizing bacteria, these organisms could not use co as a sole source of energy. among 17 studied strains of hydrogen bacteria, only those belonging to the group alcaligenes eutrophus could withstand 10% of co (v/v) in a gaseous mixture.	1979	440170
respiration-driven accumulation of c4 dicarboxylic acids by isolated membrane vesicles of paracoccus denitrificans.		1979	455122
hydrodynamic parameters of the detergent-solubilised hydrogenase from paracoccus denitrificans.	the hydrogenase from paracoccus denitrificans, which is an intrinsic membrane protein, has been solubilised from membranes by triton x-100. the partial specific volume of the solubilised protein has been determined using sucrose density gradient centrifugation in h2o and 2h2o. the values of the specific volumes of hydrogenase, measured in the presence or absence of triton x-100, are 0.73 and 0.74 ml . g-1, respectively, indicating that hydrogenase binds much less than one micelle of triton x-100 ...	1979	477660
transfer of kanamycin resistance mediated by plasmid r68.45 in paracoccus denitrificans.	plasmid r68.45 mediates the transfer of kanamycin resistance from pseudomonas aeruginosa to paracoccus denitrificans. kanamycin resistance could be transferred from one strain of p. denitrificans to another, thus opening up the possibility of using r68.45 as a sex factor in p. denitrificans.	1979	479105
[elimination of nitrous oxide by a combined bacterial culture].	the ability of bacteria to eliminate nitrous oxide (n2o) from a gaseous phase containing 20% o2 was studied. representatives of the following physiological groups were found to be incapable of removing n2o: denitrifying bacteria (paracoccus denitrificans), nitrifying bacteria (nitrosospira briensis), carboxydobacteria (pseudomonas carboxydoflava), methane oxidizing bacteria (methylosinus trichosporium), and nitrogen fixing bacteria (bacillus polymyxa). five corynebacteria-like strains were isola ...	1979	481272