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studies on brucella ovis (n.sp.), a cause of genital disease of sheep in new zealand and australia. 195613367402
placental pathology. i. placental lesions of sheep experimentally infected with brucella ovis. 196314080519
comparative distribution of brucella abortus, brucella melitensis, and brucella ovis in experimentally infected pregnant sheep. 196414266901
an indirect haemagglutination test for the detection of brucella ovis antibodies. 2. comparison of the indirect haemagglutination test with other diagnostic methods. 19645277878
pathologic and immunologic responses of the fetal lamb to brucella ovis. 19665948361
antigenic relationship of brucella ovis and brucella melitensis.immune sera were prepared in rabbits by the injection of living and acetone-killed cells of brucella ovis and smooth and rough b. melitensis. the use of whole-cell antigens in agglutination and agglutinin-absorption tests revealed little relationship between b. ovis and smooth b. melitensis, although there was extensive cross-agglutination between b. ovis and rough b. melitensis. the use of water-soluble antigens prepared from ultrasonically treated cells of the three strains revealed extensive ...19676032505
detection of precipitins against brucella ovis. 19674963422
the persistence of antibodies against brucella ovis and brucella abortus in rams following vaccination: a field study. 196716030713
homologies of deoxyribonucleic acids from brucella ovis, canine abortion organisms, and other brucella species.the bacterium that causes canine abortion has polynucleotide sequences similar, in deoxyribonucleic acid (dna)-dna homology studies, to those of brucella suis and, by inference from previous data, those of b. abortus and b. melitensis as well as b. neotomae. therefore, the organism causing canine abortion appears to be a member of the genus brucella. dna preparations from serratia marcescens, alcaligenes faecalis, and bordetella bronchiseptica, 58, 62, and 66 mole% guanine plus cytosine, respect ...19684882024
brucella ovis infection. i. an evaluation of microbiological, serological and clinical methods of diagnosis in the ram. 19685688906
brucella ovis vaccination of rams. a comparison of two commercial vaccines and two methods of vaccination. 19685688907
brucella ovis infection. 2. the infection status in breeding flocks as measured by examination of rams and the perinatal lamb mortality. 19685751641
the relation of fetal age to the character of lesions in fetal lambs infected with brucella ovis. 19685752518
[infectious epididymitis of rams caused by brucella ovis]. 19685754411
the apparent failure of a vaccine to protect rams against naturally occurring brucella ovis infection. 19695812788
phenotypic comparison of brucella ovis to the dna-homologous brucella species. 19695824903
antigenic relationship of brucella ovis to brucella abortus and brucella melitensis using the complement fixation test. 19695005098
antibody responses to brucella ovis in maternal and fetal sheep. 19694181004
the bacteriology and serology of ewes inoculated with viable brucella ovis organisms. 19705266508
preliminary report on the development of a diffusion-in-gel method for the diagnosis of ram epididymitis.a simple gel-diffusion technique is described for the diagnosis of ram epididymitis caused by brucella ovis. the results are shown to be very similar to those obtained by the complement-fixation test, which is currently the standard method of diagnosis. the method is suitable for use in the field and is expected to facilitate the control of ram epididymitis in areas where laboratory facilities are not available.19704985433
selective media for the isolation of brucella ovis. 19714930870
the incidence of brucella ovis in some kenya flocks and its relationship to clinical lesions and semen quality. 19715168506
[characteristics of cultures of brucella ovis]. 19715170299
the characteristics of "brucella ovis" (new zealand). 19715005103
experimental brucella ovis infection in ewes. 1. breeding performance of infected ewes. 19725011948
experimental brucella ovis infection in ewes. 2. correlation of infection and complement fixation titres. 19725011949
experimental brucella ovis infection in rams. 19725075203
studies of antigens for complement fixation and gel diffusion tests in the diagnosis of infections caused by brucella ovis and other brucella.sonically treated and saline-extracted antigens of brucella ovis, b. canis, b. abortus, and b. melitensis were compared in gel diffusion, complement fixation, and serum absorption tests. all the sonically extracted antigens showed cross-reactions with sera from animals infected or immunized with these species, whereas the saline-extracted antigens were specific for the surface of the rough or smooth colonial phase of the species or strain. the saline-extracted antigens of b. ovis and b. melitens ...19724624210
the incidence of brucella ovis infection in south african rams: a serological survey. 19724217833
[characteristics of the so-called "brucella ovis" (new zealand)]. 19724402417
field evaluation of the gel diffusion test for the diagnosis of ram epididymitis caused by brucella ovis.a comparison was made with the microslide gel diffusion technique, the complement fixation test, cultural isolation, and clinical examination in detecting ram epididymitis caused by brucella ovis. the results of the gel diffusion method are shown to be similar to those obtained by the complement fixation test and isolation of b. ovis from cultures of semen. the technique offers a reliable diagnostic method adaptable to field use in controlling ovine brucellosis and is more practical.19734203332
[sero-epidemical study of the hazards of occupational infections in the course of contact with brucella ovis (epizootic foci, laboratories)]. 19734732970
studies on the transmission of brucella ovis infection in rams. 19734734475
pathogenesis of epididymitis caused by brucella ovis in laboratory animals. 19734755775
[dynamics of igm and igg in rams experimentally infected with brucella ovis]. 19734799562
brucella ovis as a cause of abortion in ewes. 19744208504
comparative sensitivity of gel-diffusion and tube agglutination tests for the detection of brucella canis antibodies in experimentally infected dogs.the microslide gel-diffusion and macro-tube agglutination techniques to detect brucella canis antibodies in dogs were compared. sera from dogs experimentally infected with b. canis and a random sample of dog sera with unknown histories of exposure to this organism were examined. the results of the gel-diffusion method employing specific rough brucella saline-extract antigens of b. canis and brucella ovis were comparable to those obtained by the tube agglutination test. the easily prepared, stabl ...19744210726
an automated complement fixation test for brucella ovis in sheep. 19744524883
the complement fixation test for the diagnosis of brucella ovis infection in sheep. 19744531600
letter: brucella ovis vaccination of rams. 19751060006
duration of brucella ovis infection in ewes.twenty romney marsh ewes, some 5 to 40 days pregnant, were inoculated with a strain of brucella ovis onto the scarified mucous membrane of the vagina. establish of infection was ascertained by recovery of the organisms from blood; supramammary and inguinal lymph nodes; the vagina, after initial disappearance of the organism from this organ; and the complement fixation tests. the bacteremia was transitory or intermittent, but organisms could not be recovered from blood, or any other tissue examin ...19751126169
studies on h2s production by brucella ovis strains. 19751181906
effectivity of rev 1 vaccine in rams against brucella ovis infection.vaccination with rev 1 at four months of age protected rams against experimental infection with brucella ovis. a double vaccination did not improve immunity whereas rams vaccinated later in life showed a decreased immunity to the infection. lesions and complement fixation reactions were found to be inadequate criteria for the diagnosis of the disease in the individual ram.19751223287
[comparative diagnostic studies of epididymitis in rams caused by brucella ovis].semen samples taken from 21 rams with pronounced pathologic changes were studied in a complex way to establish the etiology contributing to the deterioration of the semen quality. the complement-fixation test was applied with blood serum and semen plasma along with the microscopic study of the semen for the excretion of brucella ovis organisms and a clinical examination. the following results were obtained: 1. the semen plasma of 90.5 per cent of the rams was positive for brucella ovis. a total ...1976951934
endotoxic activity of rough organisms of brucella species.a rough-specific antigen extracted from the rough species brucella ovis and lipopolysaccharide extracted from smooth brucella abortus demonstrated equivalent levels of activity in tests for mouse lethality and limulus lysate clotting activity. acetone-extracted whole cells of b. ovis and of b. canis and of a rough mutant of b; abortus had the same toxicity for mice, but it was not possible to extract endotoxin from b. canis by the methods used.1976971943
characteristics of semen changes during brucella ovis infection in rams.the effect of brucellosis on semen quality in rams was studied by means of artificially infecting five rams with a strain of brucella ovis isolated in kenya. infection resulted in reduced semen quality including reduced total sperm output, poor motility and a high percentage of morphological abnormalities. variation in semen quality both between and within rams appeared to be related to progress of the disease in the epididymis as well as to the distribution and severity of lesions in the reprod ...1976982755
[sperm-autophagocytosis in rams affectes with brucella ovis].studied was micrographically the semen of rams affected with infectious epididymitis admixed with leukocytes and brucella ovis organisms. the animals were cft-positive for brucellosis. it was found that in addition to the abundantly phagocytized brucellae in the cytoplasm of the polymorphonuclear leukocytes there were fairly often surrounding sporadic spermatozoa or groups of them whose flagellae and heads have penetrated into the respective leukocyte. to elucidate this phenomenon ultrasections ...19761014362
evolution and taxonomy in the genus brucella: progesterone induction of filterable forms of brucella abortus type 2 with revertant characteristics essentially indistinguishable in vitro from those of brucella ovis.an organism essentially indistinguishable from brucella ovis, as determined by conventional and manometric techniques of identifying brucella organisms, was derived in vitro from a strain of brucella abortus type 2. the basic mechanism responsible for derivation of this organism was incomplete reversion of the l-forms to their original parental characteristics. the agent used to induce the l-forms was a physiologic concentration of progesterone incorporated in the growth medium.1976816233
[ultrastructural changes in the spermatozoa of sheep infected with brucella ovis].the semen was studied of four rams of the mutton merino, suffolk, "kavkazki merino* breeds and a local crossbred race, affected with infectious epididymitis and having semen deteriorated to a various extent, in order to establish the changes in the ultrastruct of spermatozoa through electron microscopy. the following conclusions were drawn on the basis of the results obtained: 1. the changes caused by brucella ovis infection in such cases are chiefly concentrated in the head of spermatozoa. deta ...1977929971
cholinesterase activity of sperm from rams infected with brucella ovis.studied were the indices of cholinesterase activity of semen taken from 23 normal rams and 23 rams infected with brucella ovis, the latter being positive by the complement-fixation test and showing a varying deterioration of the semen production. the results obtained were processed biometrically. established were dependable differences. the cholinesterase activity of semen of brucellosis-affected rams proved four times higher than that of normal rams' semen: 39.45 +/- 5.49 microleter to2 for 1 h ...197722157
a comparison of methods for the serological diagnosis of brucella ovis infection. 1977608087
[experimental infection of goats with brucella ovis. bacteriological serological and histopathological studies].the susceptibility of adult male goats to brucella ovis infection was studied. fifteen goats and fifteen rams both of ages ranging from 22 to 34 months were inoculated conjunctivally with 10(9) cells of b. ovis strain recently isolated from a case of ram epididymitis. five goats and five rams were killed 78 days after inoculation and similar groups were killed at two month intervals thereafter. b. ovis was recovered from semen of a male goat, 33 and 61 days after inoculation. the five goats sacr ...1977614651
[ultrastructural changes in the testis and epididymis of rams with infectious epididymitis].electron microscope studies were carried out on the ultrastructural changes in the testis and the epidiymis of rams affected with infectious epididymitis, manifesting a pronounced clinical picture. the following conclusions were drawn: 1. the changes in the seminiferous tubules are characterized by a strongly expressed sensitivity of the cells at all levels. noticeable are disturbances in the normal structure of the nuclear substance of both spermatogonia and spermatocytes, vacuolation of the nu ...1978741645
testicular pathology of merino rams.the scrotal contents of 2,281 merino rams of wide age range examined at 3 abattoirs in perth, western australia, showed a 40% prevalence of rams with 1 or more gross lesions. the percentage prevalence of gross lesions found on autopsy were: adhesions 21, testicular atrophy/hypoplasia 14, testicular calcification 13, congenital cyst of the epididymis 6, cryptorchidism 4, chronic epididymitis/scrotal abscesses 2, varicocoele 2, testicular aplasia less than 1, seminoma less than 1. the prevalence o ...1978743044
serological comparison between histophilus ovis, actinobacillus seminis and brucella ovis.the serological relationships between 4 strains of histophilus ovis, the neotype strain of actinobacillus seminis and brucella ovis were examined using a cross-absorption complement-fixation technique. it was found that the 4 strains of h. ovis were serologically homologous and that an incomplete relationship existed between these organisms and a. seminis. anteriserums prepared against one strain of h. ovis and the strain of a. seminis gave a weak, apparently non-specific cross-reaction with br. ...1978743054
[experiments with vaccination against infectious epididymitis in rams].two killed vaccines were produced, differing by the adjuvant used--a hydroxide one and an oil one--employing four strains of brucella ovis. they were applied twice at an interval of 20 days to rams under experimental conditions as well as to rams in filed conditions. on the 45th day following vaccination the experimental rams were challenged with a virulent brucella ovis strain. to determine the acquired resistance the rams were investigated after the complement-fixation test, hemagglutination r ...1978749327
observations on brucella ovis cft results. 1978286955
[biochemical study of serum from sheep infected with brucella ovis].blood serum was used of 49 rams and 20 ewew infected with brucell ovis. the study on the total protein and the protein fractions of rams revealed that there existed gamma-globulinemia, the percent of the gamma-globulin rise showing a positive correlation with the morphologic changes in the testes. the alfa-globulins were found to rise immediately following the experimental infecting of the sheep for about a month, after which they came back to normal. in experimental infection the activity of th ...197883049
[aspects related to the antigenic structure and serological specificity of brucellae phylogenetically related to s- and r-forms and dissociated into r-variants].the antigenic structures and serological properties of brucellae s- and r-forms, phylogenetically differentiated, and dissociated r-variants are studied by means of gel-precipitation, immunoelectrophoresis and fixation of the complement. the brucella suis 1330s, brucella suis 1330r, brucella ovis 02 and brucella abortus 99 are involved in the experiments. both specific and general antigenic structures are established in all strains studied, but only homologous antibodies from the antigen-antibod ...197888791
epiphysitis in rams following vaccination against brucella ovis infection. 197898740
epididymitis in rams: effect of vaccination and culling on the clinical incidence of the disease.ram epidiymitis caused by brucella ovis can be effective controlled by an annual program combining rigorous culling clinically affected animals and appropriate vaccination of the remaining animals. yearling rams purchased or saved as replacement breeders should be immunized at 4-5 months of age by two injections of br. ovis bacterin spaced 3-6 weeks apart. annually, approximately one month prior to the breeding season, all rams, replacements and older breeders as well, should be carefully examin ...1979110514
comparison of agar gel precipitation and complement fixation test for the detection of brucella ovis infection.blood samples from 710 sheep raised in 20 different farms were examined for brucella ovis antibodies. thirty samples were positive with complement fixation, 35 samples with precipitation, and 58 samples with both tests. it has been concluded that the gel precipitation technique is adequate for screening but for individual testing both methods should be used simultaneously.1979113989
serodiagnosis of ram epididymitis by counterimmunoelectrophoresis, using brucella ovis surface r antigen.the counterimmunoelectrophoresis (cie) technique was developed as a diagnostic procedure for ram epididymitis caused by brucella ovis. cie test results with sera from naturally and experimentally infected male sheep compared favorably with those obtained by gel diffusion and complement fixation employing the same b. ovis surface r antigen. the main advantage of cie over gel diffusion consists in a significant reduction of the time required to detect precipitin formation, whereas both methods obv ...1979118983
actinobacillus seminis infection in sheep in the republic of south africa. iii. growth and cultural characteristics of a. seminis.bacteriological tests were done on a large number of different strains of actinobacillus seminis and also, repeatedly, on the same culture or on different cultures taken periodically from the same donor animal. these tests were also applied to strains of a. seminis representing different serological types, which in turn were compared with strains of brucella ovis. the tests as applied proved that a. seminis strains have defined, morphological, staining, cultural and biochemical properties, altho ...197995214
bacterial isolates associated with epididymitis in rams from idaho and eastern oregon flocks.testicles from 47 rams with palpable lesions of epididymitis were subjected to bacterial culture. of the testicles examined by culture technique, 49% were positive for bacterial growth. the 2 most frequent isolates were actinobacillus actinomycetemcomitans (23.4% of the testicular pairs) and brucella ovis (14.9% of the testicular pairs). heretofore a actinomycetem-comitans has not been reported as a cause of epididymitis in rams.1979453669
brucella ovis in merino rams in western australia. 1979464944
actinobacillus seminis infection in sheep in the republic of south africa. i. identification of the problem.a clinical palpation and semen smear examination of 647 rams submitted to the regional veterinary laboratory during 1967 revealed that 42 (6,5%) of these animals had clinical epididymitis or orchitis, 6 (0,9%) showed other types of genital lesions and 98 (15,1%) suffered from subclinical genital infection. a. seminis and a. seminis-like organisms were isolated from semen specimens of 18 out of 35 rams with clinical epididymitis or orchitis, 25 out of 33 rams with subclinical infection and none o ...1979551360
[comparative investigations using the complement fixation test and the agar gel immunodiffusion test to reveal the presence of brucella-ovis-antibodies (author's transl)]. 19806772421
diagnosing ovine epididymitis by immunofluorescence.antisera against actinobacillus seminis, brucella ovis and corynebacterium pseudotuberculosis were prepared in adult female goats. specific immunofluorescence was observed in cultural smears of a seminis, b ovis and c pseudotuberculosis by the direct technique and in smears of a seminis also by the indirect technique. individual organisms could be recognised. specific fluorescence of a seminis was readily detected in semen. the results indicate that immunofluorescence may offer an effective meth ...19807020228
evaluation of procedures for the diagnosis of brucella ovis infection in rams.procedures for the diagnosis of br. ovis infection in rams were evaluated by examining 10 rams artificially infected by preputial inoculation. observations were undertaken at weekly intervals for 1 year to follow changes in clinical, bacteriological and serological findings. clinical lesions were detected in 1 ram 3 weeks after inoculation and in all rams by 8 weeks; lesions were undetectable in 3 rams at the completion of the trial. the presence of inflammatory cells in semen samples was the ea ...19807436917
[pathomorphological changes in brucella ovis-infected sheep].in spontaneously infected with br. ovis rams, as well as in experimentally infected with the same infection sheep and rams, besides the morphological changes in the epididymis with some part of the animals, we also observed granulomas in the lungs with a specific morphological structure. the presence of br. ovis was proved in a number of organs such as the epididymis, the seminal ampullae, the lungs, the kidneys, the liver and the myocardium. in spite of the presence of bacteria no morphological ...19807222458
the pathology of bacterial infection of the genitalia in rams.details are given of the macroscopic and histopathological changes brought about by infection of the genitalia of rams by bacteria other than brucella ovis. lesions of the seminal vesicles and ampullae are described which, in addition to the clinically evident lesions of the testes and epididymis, could be an important reasons for impaired fertility. the name "bacterial infection of the genitalia", abbreviated to big, is suggested as a more appropriate designation for this condition than "ram ep ...19807231922
protection of rams against brucella ovis infection by brucella melitensis rev. 1 vaccine. 19817324641
binding of bacteria from the genus brucella to human b lymphocytes.in previous studies, we have shown that various lymphocyte subpopulations bind different strains of bacteria of different genera and species. among these bacteria was a strain of brucella melitensis which bound to all human b lymphocytes. to determine whether the binding of b. melitensis to human b lymphocytes was strain, species, or genus characteristic, we tested the binding of b. melitensis, brucella abortus, brucella ovis, brucella suis, brucella canis and brucella neotomae to human normal a ...19816783546
the complement fixation test for brucella ovis. 19816942344
demonstration of brucella ovis infection in a bilateral cryptorchid ram. 19816119072
ovine contagious epididymitis: a review.ovine contagious epididymitis is predominantly associated with brucella ovis, actinobacillus seminis and a variety of organisms including histophilus ovis. transmission occurs venereally or by homosexual activity and, in the case of actinobacillus seminis, ewe to lamb transmission is probably important. a chronic infection, mainly in the cauda epididymis may result in formation of spermatic granulomata and a reduction in ram fertility. eradication of ovine brucellosis can be achieved by serologi ...19826762755
sensitivity and specificity of two microtitre complement fixation tests for the diagnosis of brucella ovis infection in rams.an investigation was made into microtitre complement fixation test (cft) procedures suitable for the serological diagnosis of naturally occurring brucella ovis infection in rams. a procedure similar to the australian standard procedure for bovine brucellosis was unsatisfactory when applied to sheep. modification of the procedure by use of an initial serum and anticomplementary control dilution of 1:8 and increasing complement fixation time to 60 minutes at 37 degrees c, greatly improved the effi ...19827082234
epidemiological studies on ovine brucellosis in selected ram flocks.the epidemiology of ovine brucellosis was investigated in 6 ram flocks in which anomalous reactions to the complement fixation test was recorded. it was shown that rams can become infected as young as 4 months of age. naturally infected animals have an epididymitis and excrete brucella ovis in their semen or they may only show a serological response for a short time then recover. the base line titres of chronically infected animals stay relatively constant. the importance of abortive infection a ...19827150131
efficacy of brucella melitensis rev. 1 vaccine against brucella ovis infection in rams.the efficacy of brucella melitensis strain rev. 1 vaccine against a brucella ovis experimental infection was judged on 30 rams, 15 of which had received 8.5 x 10(8) rev. 1 at the age of 4 months. all were challenged 9 months later by conjunctival route with 5 x 10(8) b. ovis. the 30 rams were killed three months after challenge and were autopsied. genital and other organs and lymph-nodes were searched for b. ovis. only a few organs and lymph-nodes of three vaccinated rams shed b. ovis, contrary ...19827168540
the complement fixation test for brucella ovis. 198216030829
further practitioner comment on brucella ovis. 198216030847
brucella ovis eradication. 198216030863
serology as an aid to diagnosis: uses and abuses.the importance of correct interpretation of serological test results, common sources of error and problems associated with tests are discussed. in bovine brucellosis, a disease which is ideally suited to serological diagnosis, foetal contact with infection may cause the calf to be a serologically negative carrier. the immune tolerant animal resulting from foetal contact with virus is a major problem in the serological detection of border disease. in johnes' disease and to a lesser extent in bruc ...198216030880
ram epididymitis: a clinical report.two hundred and five ram lambs originating from several sources were assembled for a ram performance trial. all rams were immunized with a commercial brucella ovis bacterin. four rams developed clinical evidence of epididymitis during the 150 day trial. actinobacillus seminis was identified as the causative agent.198216725695
the brucella ovis complement fixation test. 198316030984
the complement fixation test for the diagnosis of brucella ovis infection in rams--an animal health division report.complement fixation tests using three b. ovis antigen preparations in warm fixation tests (wcft) and cold fixation (ccft) tests were done on 541 ram sera. semen samples from the same rams were examined culturally to identify b. ovis excretors. the ccft, using an antigen prepared by heat extraction of b. ovis cells, had a sensitivity of 97% in 124 rams which were shedding b.ovis. the specificity was 99% in 144 rams from non-infected flocks. seventy-seven per cent of 156 rams which reacted to this ...198316030997
association of sexual experience with isolation of various bacteria in cases of ovine epididymitis.testicles from rams in flocks experiencing ram epididymitis in idaho and eastern oregon were cultured. twenty-six breeding rams from 6 flocks were cultured and only brucella ovis was isolated. virgin rams (65) harbored numerous species of small fastidious gram-negative rods, including actinobacillus actinomycetemcomitans, haemophilus spp, moraxella spp, and pasteurella spp. thus, there appeared to be 2 separate disease entities, dependent on sexual experience of the animal.19836833070
brucella ovis-induced abortion in ewes.aborted fetuses and placental tissue from ewes in 2 flocks were submitted for diagnostic examinations. brucella ovis was isolated from fetal lung or abomasal contents, or both. placentitis was a consistent gross lesion. microscopic lesions included suppurative pneumonia and placentitis.19836618987
interaction of brucella ovis with ovine tissue extracts. 19836636477
comparison of different antigenic preparations for the detection of ovine serum antibodies against brucella ovis by elisa.an enzyme-linked immunosorbent assay has been developed for the detection of antibodies against brucella ovis using serum from control rams (con-s), naturally infected rams (inf-s), rams inoculated intravenously with b. ovis (iv-s) and rams vaccinated intramuscularly (im-s). the serum was titrated by serial double dilutions from 1/25 to 1/25,600 against whole bacteria, b. ovis lipopolysaccharide and a detergent-extracted component of the outer membrane complex of b. ovis as antigens immobilised ...19836651682
evaluation of surface components of brucella ovis as antigens for the detection of precipitin antibody in serums from artificially exposed rams.surface components of brucella ovis obtained by gentle physical shearing were tested as a potentially useful source of reagent for selective serological diagnosis. these antigens were used in a radial immunodiffusion (rid) test against serum from rams which had been inoculated with infective semen containing b. ovis by one of 4 routes namely mating rams with ewes previously inoculated intravaginally with infective semen, or by direct inoculation in the prepuce, rectum or nasal passage. loosely a ...19836651683
comparison of the enzyme-linked immunosorbent assay and complement fixation test for detecting brucella ovis antibodies in sheep.a solid-phase, indirect, enzyme-linked immunosorbent assay (elisa) was compared with the microtitre complement fixation test for detecting brucella ovis antibodies in 220 ram sera. the elisa was more sensitive than the complement fixation test; it demonstrated antibodies in 11 sera from known infected or vaccinated rams that were complement fixation test negative. no false positives were recorded with the elisa and, in 36 sera positive to both tests, the elisa titres were consistently higher tha ...19836359661
enzyme-linked immunosorbent assay for brucella ovis specific antibody in ram sera.an enzyme-linked immunosorbent assay (elisa) for the detection of brucella ovis specific antibody in ram serum was compared with the currently employed complement fixation test (cft). rabbit anti-sheep igg coupled to horseradish peroxidase was used as the antibody-enzyme conjugate and 2,2'-azino-di[3-ethylbenzthiazolin sulphonate (6)] as the substrate. the ratio of the optical density at 414 nm for positive and negative control sera (p/n ratio) was used to optimise the parameters of the test. ra ...19846371959
characterization of brucella ovis lipopolysaccharide and its use for diagnosis of ram epididymitis by enzyme-linked immunosorbent assay.rough lipopolysaccharide, extracted by a mixture of phenol, chloroform, and petroleum ether from freeze-dried brucella ovis cells with a yield of 0.71%, contained relatively small amounts of protein and nucleic acid contaminants as compared with lipopolysaccharides from other brucellae. the crude lipopolysaccharide was suitable as a diagnostic antigen in an enzyme-linked immunosorbent assay for the sensitive and specific detection of ram epididymitis caused by b. ovis infection. in comparative s ...19846394618
immunochemical characterization of rough brucella lipopolysaccharides.lipopolysaccharides (lps) were extracted from rough strains of brucella abortus and brucella melitensis and from strains of the naturally occurring rough species brucella ovis and brucella canis. brucella rough lipopolysaccharides (r-lps) were readily distinguished from brucella smooth lipopolysaccharides (s-lps) and enterobacterial r-lps, by their chemical, physical, and serological characteristics. b. ovis r-lps was differentiated from b. abortus, b. melitensis, and b. canis r-lps by its react ...19846421737
differentiation of brucella ovis from brucella abortus by gas-liquid chromatographic analysis of cellular fatty acids.the cellular fatty acid composition of brucella ovis and brucella abortus strains was determined by gas-liquid chromatography. both species were characterized by the presence of fatty acids 16:0, 17:0, 17:0 cyclopropane, 18:0, 18:1, and 19:0 cyclopropane; b. ovis also contained some 15:0. there were differences in the relative proportions of the fatty acids present, and it was possible to differentiate b. ovis from b. abortus on the basis of the absence of 15:0, lower concentrations of 17:0 and ...19846432837
protection of rams against epididymitis by a brucella ovis-vitamin e adjuvant vaccine.rams vaccinated at 7 and 8 months of age with a b. ovis-vitamin e adjuvant vaccine had increased antibody titers compared with freund's incomplete adjuvant or commercial bacterin vaccinated rams. the percent overall infectivity in an experimental infection of b. ovis-vitamin e adjuvant vaccinated rams was 22% compared to 44% for b. ovis-freund's incomplete adjuvant or bacterin vaccinated rams and 67% for control.19846506451
histopathology of experimental brucellosis in rams following vaccination with brucella ovis.lesions induced by inoculation of brucella ovis into the epididymis were compared in rams previously vaccinated with b. ovis bacterin and unvaccinated rams. inoculation of killed b. ovis did not produce significant lesions in either group whereas prior vaccination exacerbated epididymal lesions following inoculation of live b. ovis. increased numbers of neutrophils, macrophages and lymphocytes were present in the interstitium and neutrophilic infiltration of the epididymal duct epithelium and in ...19846529395
epididymitis of rams in the central and southern districts of the orange free state.scrotal palpation, microscopical examination of semen smears and the application of bacteriological techniques, revealed the incidence of actinobacillus seminis and brucella ovis infection of rams in the central and southern districts of the orange free state to be 2,9% and 4,3%, respectively. more dorper than merino rams were affected. although clinically detectable epididymitis was found in 5,9% of rams examined, infection with a. seminis and b. ovis, as measured by the presence of neutrophils ...19846533305
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