Publications

sodium ion-proton antiport in a marine bacterium.	alteromonas haloplanktis ejected protons in response to a brief respiratory pulse; the rate of decay of the resulting ph change was accelerated when na+ was present in the suspension medium. the addition of an anaerobic nacl solution to an essentially na+-free anaerobic bacterial suspension induced the acidification of the suspension medium. these results and others discussed provide substantial evidence for the existence of an na+-h+ antiporter in this organism.	1978	26666
composition of the fractions separated by polyacrylamide gel electrophoresis of the lipopolysaccharide of a marine bacterium.	the sugar composition of lipopolysaccharide (lps) isolated from whole cells of alteromonas haloplanktis 214 (previously referred to as marine pseudomonas b-16, atcc 19855), variant 3, of the lipid a, core, and side-chain fractions derived from it, and of the lps fractions (lps i, ii, and iii) obtained by subjecting it to preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis has been determined. conditions optimum for the release of constituent monosaccharides by hydrolysis were e ...	1978	101510
osmotic effects of membrane permeability in a marine bacterium.	when cells of alteromonas haloplanktis 214 (atcc 19855) were preloaded with alpha-[(14)c]aminoisobutyric acid or the k(+) in the cells was labeled with (42)k by incubation in a buffered salt solution containing 0.05 m mgso(4), 0.01 m kcl, and 0.3 m nacl, the cells retained their radioactivity when resuspended in the same salt solution. when nacl was omitted from the solution, 80 to 90% of the radioactivity was lost from the cells. cells suspended at intermediate concentrations of nacl also lost ...	1978	641005
heterogeneity and distribution of lipopolysaccharide in the cell wall of a gram-negative marine bacterium.	lipopolysaccharide (lps) extracted from alteromonas haloplanktis 214, variants 1 and 3, separated into three fractions when subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the fractions appeared in the gels as bands which stained for carbohydrate with the periodate-schiff reagent. variant 1, a smooth variant of the organism, and variant 3, a rough colonial variant, produced identical banding patterns. under similar conditions, lps from neisseria meningitidis sdic, escheri ...	1978	711665
energy coupling to k+ transport in a marine bacterium.	cells of the marine bacterium alteromonas haloplanktis 214 atcc 19855 (previously referred to as marine pseudomonad b-16) were depleted of k+ by washing with 0.1 m mgso4. washing with 0.05 m mgso4 lowered the vmax for k+ transport compared with washing with 0.1 m with 0.05 but did not change the km, while washing with lower concentrations of mgso4 caused loss of ultraviolet-absorbing material from the cells. k+ uptake was a strictly aerobic process and was accompanied by proton release. when an ...	1980	6450627
sodium ion-substrate symport in a marine bacterium.	anaerobic suspensions of alteromonas haloplanktis accumulated alpha-aminoisobutyric acid, by a sodium-dependent process, in response to an artificially imposed membrane potential in the presence or absence of a transmembrane chemical gradient of sodium. these results suggest that the transport of alpha-aminoisobutyrate by this organism occurs via na+-substrate symport.	1980	7380801
lipid composition and chemotaxonomy of pseudomonas putrefaciens (alteromonas putrefaciens).	the major polar lipids in cells of pseudomonas putrefaciens ncib 10472 grown on nutrient agar were phosphatidylethanolamine, phoisphatidylglycerol, a glucosyldiacylglycerol, a glucuronosyldiacylglycerol and an ornithine amide lipid. an additional phospholipid, tentatively identified as acyl phosphatidylglycerol or bis-phosphatidic acid, was a trace component of the wall lipids from broth cultures, which lacked the glycolipids and the ornithine amide lipid. the wall lipids from broth cultures of ...	1980	7441198
growth characteristics af low na+ concentration and the stability of the na+ requirement of a marine bacterium.	studies of the marine bacterium alteromonas haloplanktis 214 (formerly referred to as marine pseudomonad b-16) showed that as the na+ concentration in the growth medium decreased from 230 to 34 mm, the lowest concentration permitting growth, the length of the lag period preceding exponential growth increased. once growth had begun, except for a slight reduction in rate of growth at 34 mm na+, the generation time and extent of growth remained essentially constant over the range of na+ concentrati ...	1981	7237281
effect of growth at low na+ concentrations on the capacity of a marine bacterium to establish ion gradients and transport alpha-aminoisobutyric acid.	cells of a histidine-auxotrophic, streptomycin-resistant mutant of marine bacterium alteromonas haloplanktis 214 were grown at or near the lowest concentration of na+ permitting growth (30-33 mm na+). when suspended in solutions containing 10 mmkcl and either 30, 100, or 300 mm nacl, the intracellular to extracellular k+ ratios were similar to those obtained with cells of the parent organism grown at more nearly optimum na+ concentrations, whereas the na+ ratios were somewhat larger. cells of th ...	1981	7237282
relationship between ion requirements for respiration and membrane transport in a marine bacterium.	intact cells of the marine bacterium alteromonas haloplanktis 214 oxidized nadh, added to the suspending medium, by a process which was stimulated by na+ or li+ but not k+. toluene-treated cells oxidized nadh at three times the rate of untreated cells by a mechanism activated by na+ but not by li+ or k+. in the latter reaction, k+ spared the requirement for na+. intact cells of a. haloplanktis oxidized ethanol by a mechanism stimulated by either na+ or li+. the uptake of alpha-aminoisobutyric ac ...	1984	6690427
expression of genes from the marine bacterium alteromonas haloplanktis 214 in escherichia coli k-12.	dna from the marine bacterium alteromonas haloplanktis 214 was partially digested with sau 3a and inserted into the bam hi site of the cloning vector pbr322. the ligation mixture was used to transform escherichia coli hb101. the gene bank plasmid preparation obtained was used to transform escherichia coli k-12 strain eo2717, an organism auxotrophic for histidine, arginine, adenine, uracil and thiamin. prototrophic transformants for each of the five metabolites were isolated using appropriate min ...	1985	3899044
cloning in escherichia coli k-12 of a na+-dependent transport system from a marine bacterium.	the transport of d-alanine by escherichia coli k-12 neither requires nor is stimulated by na+. the transport of d-alanine by the marine bacterium alteromonas haloplanktis 214 requires na+ specifically. mutants of e. coli which were unable to transport d-alanine were isolated by enrichment for d-cycloserine resistance. one of the mutants was transformed with a gene bank of a. haloplanktis chromosomal dna. two transformants, e. coli rm1(ppm1) and e. coli rm1(ppm2) were able to transport d-alanine ...	1986	3512524
bisucaberin, a new siderophore, sensitizing tumor cells to macrophage-mediated cytolysis. i. taxonomy of the producing organism, isolation and biological properties.	alteromonas haloplanktis strain sb-1123 isolated from deep-sea mud produced a new siderophore, bisucaberin. bisucaberin rendered tumor cells susceptible to cytolysis mediated by murine peritoneal macrophages which were elicited by proteose peptone and not yet activated by lymphokine. bisucaberin exerted its sensitizing activity by both the preincubation with tumor cells and the addition to co-culture of macrophages and tumor cells. the activity of bisucaberin was specifically inhibited by ferric ...	1987	2962972
variation in quantitative requirements for na for transport of metabolizable compounds by the marine bacteria alteromonas haloplanktis 214 and vibrio fischeri.	the rates of uptake by alteromonas haloplanktis of 19 metabolizable compounds and by v. fischeri of 16 of 17 metabolizable compounds were negligible in the absence of added alkali-metal cations but rapid in the presence of na. only d-glucose uptake by v. fischeri occurred at a reasonable rate in the absence of alkali-metal cations, although the rate was further increased by added na, k, or li. quantitative requirements for na for the uptake of 11 metabolites by a. haloplanktis and of 6 metabolit ...	1987	16347378
sensitivity of some marine bacteria, a moderate halophile, and escherichia coli to uncouplers at alkaline ph.	the inhibitory effects of uncouplers on amino acid transport into three marine bacteria, vibrio alginolyticus 118, vibrio parahaemolyticus 113, and alteromonas haloplanktis 214, into a moderate halophile, vibrio costicola nrc 37001, and into escherichia coli k-12 were found to vary depending upon the uncoupler tested, its concentration, and the ph. higher concentrations of all of the uncouplers were required to inhibit transport at ph 8.5 than at ph 7.0. the protonophore carbonyl cyanide m-chlor ...	1988	3045092
effect of na concentration and nutritional factors on the lag phase and exponential growth rates of the marine bacterium deleya aesta and of other marine species.	growth of the marine bacterium deleya aesta in a succinate minimal medium showed increasingly long lag phases as na was decreased below the optimum (200 to 500 mm). the minimum na concentration permitting growth consistently was 15 mm. supplementation of the medium with khco(3) (as a source of co(2)) or yeast extract, especially in combination, reduced the lag phase, increased the rate of exponential growth, and allowed growth at 8 mm na. khco(3) did not reduce the lag period but did increase th ...	1989	16347969
[detection of aquatic microorganisms from the black sea--producers of restriction endonucleases].	300 clones of microorganisms isolated at different stations and from different depths in the black sea were screened for restriction endonucleases production. the production of restriction endonucleases was found in 17 clones screened. three of them were identified to be alteromonas haloplanktis b1. restriction endonuclease ahab1 is an isoshizomer of sau961. an identified alteromonas haloplanktis clone b8 produces ahab8i restriction endonuclease the prototype to which is kpni. of the clones isol ...	1990	1964718
identification and sequence of a na(+)-linked gene from the marine bacterium alteromonas haloplanktis which functionally complements the daga gene of escherichia coli.	a 4.0 kb fragment from a plasmid genomic dna library of the marine bacterium alteromonas haloplanktis atcc 19855 was found in the presence of na+ to complement the daga gene of escherichia coli. we have completely sequenced this fragment and the position of the na(+)-linked d-alanine glycine permease gene (daga) on the fragment has been determined by complementation. the predicted carrier protein consists of 542 amino acid residues (m(r) 58,955). its hydropathy profile suggests it is composed of ...	1992	1447975
[structure of the repeating link of the acid polysaccharide of alteromonas haloplanktis kmm 156].	an acidic capsular and an o-specific polysaccharide were isolated from the marine microorganism alteromonas haloplanktis kmm 156. both polysaccharides have the identical structure and are built up of tetrasaccharide repeating units, containing two residues of l-rhamnose as well as a 2-acetamido-2-deoxy-d-glucose and a 3-o-[(r)-1-carboxyethyl]-d-glucose (glc3lac) residue. on the basis of methylation studies, 1h- and 13c-nmr-spectroscopy including nuclear overhauser effect and two-dimensional hete ...	1993	8489533
flow cytometric analysis of the cellular dna content of salmonella typhimurium and alteromonas haloplanktis during starvation and recovery in seawater.	flow cytometry was used to investigate the heterogeneity of the dna content of salmonella typhimurium and alteromonas haloplanktis cells that were starved and allowed to recover in seawater. hoechst 33342 (bisbenzimide) was used as a dna-specific dye to discriminate between dna subpopulations. the dna contents of both strains were heterogeneous during starvation. s. typhimurium cells contained one or two genomes, and a. haloplanktis cells contained up to six genomes. s. typhimurium genomes were ...	1994	7811075
in situ biodegradation of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in natural waters.	the biodegradation of samples of poly(3-hydroxybutyrate)(p(3hb)), poly(3-hydroxybutyrate-co-10%-3-hydroxyvalerate)(p(3hb-co-10 %-3hv)), and poly 3-hydroxybutyrate-co-20%-3-hydroxyvalerate)(p(3hb-co-20%- 3hv)) was investigated in situ in natural waters. the degradation was studied by decrease in mass, molecular weight, and tensile strength. in two freshwater ponds the polymers were slowly degraded. after half a year of submersion the mass loss was less than 7%. after 358 days in a freshwater cana ...	1995	7606659
crystallization and preliminary x-ray diffraction studies of alpha-amylase from the antarctic psychrophile alteromonas haloplanctis a23.	a cold-active alpha-amylase was purified from culture supernatants of the antarctic psychrophile alteromonas haloplanctis a23 grown at 4 degrees c. in order to contribute to the understanding of the molecular basis of cold adaptations, crystallographic studies of this cold-adapted enzyme have been initiated because a three-dimensional structure of a mesophilic counterpart, pig pancreatic alpha-amylase, already exists. alpha-amylase from a. haloplanctis, which shares 53% sequence identity with pi ...	1996	8897615
the marine bacterium pseudoalteromonas haloplanktis has a complex genome structure composed of two separate genetic units.	the genome size of pseudoalteromonas haloplanktis, a ubiquitous and easily cultured marine bacterium, was measured as a step toward estimating the genome complexity of marine bacterioplankton. to determine total genome size, we digested p. haloplanktis dna with the restriction endonucleases notl and sfil, separated the fragments using pulsed-field gel electrophoresis (pfge), and summed the sizes of the fragments. the p. haloplanktis genome was 3512 +/- 112 kb by notl digestion and 3468 +/- 54.1 ...	1996	8973910
nucleotide sequence of a gene encoding an organophosphorus nerve agent degrading enzyme from alteromonas haloplanktis.	organophosphorus acid anhydrolases (opaa) catalyzing the hydrolysis of a variety of toxic organophosphorus cholinesterase inhibitors offer potential for decontamination of g-type nerve agents and pesticides. the gene (opa) encoding an opaa was cloned from the chromosomal dna of alteromonas haloplanktis atcc 23821. the nucleotide sequence of the 1.7 -kb dna fragment contained the opa gene (1.3 kb) and its flanking region. we report structural and functional similarity of opaas from a. haloplankti ...	1997	9079288
pseudoalteromonas antarctica sp. nov., isolated from an antarctic coastal environment.	the taxonomic characteristics of five bacterial strains which were isolated from antarctic coastal marine environments were studied. these bacteria were psychrotrophic, aerobic, and gram negative with polar flagella. the g + c contents of the dnas of these strains were 41 to 42 mol%. the antarctic strains were phenotypically distinct from the previously described pseudoalteromonas type species. dna-dna hybridization experiments revealed that the new strains were closely related to each other but ...	1997	9103620
cloning and nucleotide sequence of the gyrb gene of vibrio parahaemolyticus and its application in detection of this pathogen in shrimp.	because biochemical testing and 16s rrna sequence analysis have proven inadequate for the differentiation of vibrio parahaemolyticus from closely related species, we employed the gyrase b gene (gyrb) as a molecular diagnostic probe. the gyrb genes of v. parahaemolyticus and closely related vibrio alginolyticus were cloned and sequenced. oligonucleotide pcr primers were designed for the amplification of a 285-bp fragment from within gyrb specific for v. parahaemolyticus. these primers recognized ...	1998	9464408
expression of psychrophilic genes in mesophilic hosts: assessment of the folding state of a recombinant alpha-amylase.	alpha-amylase from the antarctic psychrophile altermonas haloplanktis is synthesized at 0 +/- 2 degrees c by the wild strain. this heat-labile alpha-amylase folds correctly when overexpressed in escherichia coli, providing the culture temperature is sufficiently low to avoid irreversible denaturation. in the described expression system, a compromise between enzyme stability and e. coli growth rate is reached at 18 degrees c.	1998	9501457
crystal structures of the psychrophilic alpha-amylase from alteromonas haloplanctis in its native form and complexed with an inhibitor.	alteromonas haloplanctis is a bacterium that flourishes in antarctic sea-water and it is considered as an extreme psychrophile. we have determined the crystal structures of the alpha-amylase (aha) secreted by this bacterium, in its native state to 2.0 angstroms resolution as well as in complex with tris to 1.85 angstroms resolution. the structure of aha, which is the first experimentally determined three-dimensional structure of a psychrophilic enzyme, resembles those of other known alpha-amylas ...	1998	9541387
phne and glpt genes enhance utilization of organophosphates in escherichia coli k-12.	wild-type escherichia coli k-12 strain ja221 grows poorly on low concentrations (< or = 1 mm) of diisopropyl fluorophosphate and its hydrolysis product, diisopropyl phosphate (dipp), as sole phosphorus sources. spontaneous organophosphate utilization (opu) mutants were isolated that efficiently utilized these alternate sources of phosphate. a genomic library was constructed from one such opu mutant, and two genes were isolated that conferred the opu phenotype to strain ja221 upon transformation. ...	1998	9647836
effectiveness of sytox green stain for bacterial viability assessment.	the effectiveness of sytox green nucleic acid stain for measuring bacterial viability was tested on starved populations of escherichia coli and salmonella typhimurium. this stain underestimates the fraction of dead cells within starved populations containing cells with damaged nucleic acids or membranes. its application to natural samples should be considered with caution.	1998	9647851
structure of a highly acidic o-specific polysaccharide of lipopolysaccharide of pseudoalteromonas haloplanktis kmm 223 (44-1) containing l-iduronic acid and d-quinhb4nhb.	an acidic o-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide isolated by phenol-water extraction of pseudoalteromonas haloplanktis strain kmm 223 (44-1). l-iduronic acid (idoa) was found to be a component of the polysaccharide and identified by nmr spectroscopy and after carboxyl-reduction followed by acid hydrolysis and acetylation, by glc-ms as 2,3,4-tri-o-acetyl-1,6-anhydroidose. on the basis of 1h and 13c nmr spectroscopic studies, including 1d noe, 2d ...	1998	9675369
algicidal effects of a novel marine pseudoalteromonas isolate (class proteobacteria, gamma subdivision) on harmful algal bloom species of the genera chattonella, gymnodinium, and heterosigma.	during a bacterial survey of the huon estuary in southern tasmania, australia, we isolated a yellow-pigmented pseudoalteromonas strain (class proteobacteria, gamma subdivision), designated strain y, that had potent algicidal effects on harmful algal bloom species. this organism was identified by 16s rrna sequencing as a strain with close affinities to pseudoalteromonas peptidysin. this bacterium caused rapid cell lysis and death (within 3 h) of gymnodinoids (including gymnodinium catenatum) and ...	1998	9687434
genetic diversity of the biofilm covering montacuta ferruginosa (mollusca, bivalvia) as evaluated by denaturing gradient gel electrophoresis analysis and cloning of pcr-amplified gene fragments coding for 16s rrna.	the shell of the bivalve montacuta ferruginosa, a symbiont living in the burrow of an echinoid, is covered with a rust-colored biofilm. this biofilm includes different morphotypes of bacteria that are encrusted with a mineral rich in ferric ion and phosphate. the aim of this research was to determine the genetic diversity and phylogenetic affiliation of the biofilm bacteria. also, the possible roles of the microorganisms in the processes of mineral deposition within the biofilm, as well as their ...	1998	9726898
kinetic bias in estimates of coastal picoplankton community structure obtained by measurements of small-subunit rrna gene pcr amplicon length heterogeneity	marine bacterioplankton diversity was examined by quantifying natural length variation in the 5' domain of small-subunit (ssu) rrna genes (rdna) amplified by pcr from a dna sample from the oregon coast. this new technique, length heterogeneity analysis by pcr (lh-pcr), determines the relative proportions of amplicons originating from different organisms by measuring the fluorescence emission of a labeled primer used in the amplification reaction. relationships between the sizes of amplicons and ...	1998	9797317
diversity of free-living and attached bacteria in offshore western mediterranean waters as depicted by analysis of genes encoding 16s rrna.	in a previous study (s. g. acinas, f. rodríguez-valera, and c. pedrós-alió, fems microbiol. ecol. 24:27-40, 1997), community fingerprinting by 16s rdna restriction analysis applied to mediterranean offshore waters showed that the free-living pelagic bacterial community was very different from the bacterial cells aggregated or attached to particles of more than about 8 micrometer. here we have studied both assemblages at three depths (5, 50, and 400 m) by cloning and sequencing the 16s rdna obtai ...	1999	9925576
cold-active serine alkaline protease from the psychrotrophic bacterium shewanella strain ac10: gene cloning and enzyme purification and characterization.	the gene encoding serine alkaline protease (sapsh) of the psychrotrophic bacterium shewanella strain ac10 was cloned in escherichia coli. the amino acid sequence deduced from the 2,442-bp nucleotide sequence revealed that the protein was 814 amino acids long and had an estimated molecular weight of 85,113. sapsh exhibited sequence similarities with members of the subtilisin family of proteases, and there was a high level of conservation in the regions around a putative catalytic triad consisting ...	1999	9925590
a cold-active glucanase from the ruminal bacterium fibrobacter succinogenes s85.	we previously characterized two endoglucanases, celg and egd, from the mesophilic ruminal anaerobe fibrobacter succinogenes s85. further comparative experiments have shown that celg is a cold-active enzyme whose catalytic properties are superior to those of several other intensively studied cold-active enzymes. it has a lower temperature optimum, of 25 degrees c, and retains about 70% of its maximum activity at 0 degrees c, while egd has a temperature optimum of 35 degrees c and retains only abo ...	1999	10049853
determination of total protein content of bacterial cells by sypro staining and flow cytometry.	an assay has been developed for measuring protein biomass of marine planktonic bacteria by flow cytometry. the method was calibrated by using five species of bacteria (an arcobacter sp., a cytophaga sp., an oceanospirillum sp., a pseudoalteromonas sp., and a vibrio sp.) recently isolated from seawater samples and grown in culture at different temperatures. the intensity of sypro-protein fluorescence of these bacteria strongly correlated with their total protein content, measured by the bicinchon ...	1999	10388732
excision of is492 requires flanking target sequences and results in circle formation in pseudoalteromonas atlantica.	the gram-negative marine bacterium pseudoalteromonas atlantica produces extracellular polysaccharide (eps) that is important in biofilm formation by this bacterium. insertion and precise excision of is492 at a locus essential for extracellular polysaccharide production (eps) controls phase variation of eps production in p. atlantica. examination of is492 transposition in p. atlantica by using a pcr-based assay revealed a circular form of is492 that may be an intermediate in transposition or a te ...	1999	10438765
marine bacterial isolates display diverse responses to uv-b radiation.	the molecular and biological consequences of uv-b radiation were investigated by studying five species of marine bacteria and one enteric bacterium. laboratory cultures were exposed to an artificial uv-b source and subjected to various post-uv irradiation treatments. significant differences in survival subsequent to uv-b radiation were observed among the isolates, as measured by culturable counts. uv-b-induced dna photodamage was investigated by using a highly specific radioimmunoassay to measur ...	1999	10473381
cold-adapted alanine dehydrogenases from two antarctic bacterial strains: gene cloning, protein characterization, and comparison with mesophilic and thermophilic counterparts.	the genes encoding nad(+)-dependent alanine dehydrogenases (aladhs) (ec 1.4.1.1) from the antarctic bacterial organisms shewanella sp. strain ac10 (shealadh) and carnobacterium sp. strain st2 (caraladh) were cloned and expressed in escherichia coli. of all of the aladhs that have been sequenced, shealadh exhibited the highest level of sequence similarity to the aladh from the gram-negative bacterium vibrio proteolyticus (vpraladh). caraladh was most similar to aladhs from mesophilic and thermoph ...	1999	10473410
community size and metabolic rates of psychrophilic sulfate-reducing bacteria in arctic marine sediments.	the numbers of sulfate reducers in two arctic sediments with in situ temperatures of 2.6 and -1.7 degrees c were determined. most-probable-number counts were higher at 10 degrees c than at 20 degrees c, indicating the predominance of a psychrophilic community. mean specific sulfate reduction rates of 19 isolated psychrophiles were compared to corresponding rates of 9 marine, mesophilic sulfate-reducing bacteria. the results indicate that, as a physiological adaptation to the permanently cold arc ...	1999	10473441
probiotic bacteria as biological control agents in aquaculture.	there is an urgent need in aquaculture to develop microbial control strategies, since disease outbreaks are recognized as important constraints to aquaculture production and trade and since the development of antibiotic resistance has become a matter of growing concern. one of the alternatives to antimicrobials in disease control could be the use of probiotic bacteria as microbial control agents. this review describes the state of the art of probiotic research in the culture of fish, crustaceans ...	2000	11104813
a functional-phylogenetic classification system for transmembrane solute transporters.	a comprehensive classification system for transmembrane molecular transporters has been developed and recently approved by the transport panel of the nomenclature committee of the international union of biochemistry and molecular biology. this system is based on (i) transporter class and subclass (mode of transport and energy coupling mechanism), (ii) protein phylogenetic family and subfamily, and (iii) substrate specificity. almost all of the more than 250 identified families of transporters in ...	2000	10839820
beta-cyanoalanine production by marine bacteria on cyanide-free medium and its specific inhibitory activity toward cyanobacteria.	in screening the culture broth of marine bacteria collected at yap (micronesia), palau (belau), and okinawa (the southwest islands of japan) for antimicroalgal activity, 37 out of 2,594 bacterial isolates tested were found to produce anticyanobacterial substances against oscillatoria amphibia nies-361. one strain, c-979, identified as a vibrio sp., was selected and cultured in 2.4 liters of marine broth 2216 to identify the bioactive compound produced by the strain. the purified very hydrophilic ...	2000	10653741
the alpha-amylase gene amyh of the moderate halophile halomonas meridiana: cloning and molecular characterization.	two types of tn1732-induced mutants defective in extracellular amylase activity were isolated from the moderate halophile halomonas meridiana dsm 5425. type i mutants displayed amylase activity in the periplasm, and were unable to use any of the carbon sources tested, including starch and its hydrolysis product maltose. the type ii mutant was affected in the gene responsible for the synthesis of the extracellular alpha-amylase. this gene (amyh) was isolated by functional complementation of mutan ...	2000	10784044
aspartate aminotransferase from the antarctic bacterium pseudoalteromonas haloplanktis tac 125. cloning, expression, properties, and molecular modelling.	the gene encoding aspartate aminotransferase from the psychrophilic bacterium pseudoalteromonas haloplanktis tac 125 was cloned, sequenced and overexpressed in escherichia coli. the recombinant protein (phaspat) was characterized both at the structural and functional level in comparison with the e. coli enzyme (ecaspat), which is the most closely related (52% sequence identity) bacterial counterpart. phaspat is rapidly inactivated at 50 degrees c (half-life = 6.8 min), whereas at this temperatur ...	2000	10785402
a dna ligase from the psychrophile pseudoalteromonas haloplanktis gives insights into the adaptation of proteins to low temperatures.	the cloning, overexpression and characterization of a cold-adapted dna ligase from the antarctic sea water bacterium pseudoalteromonas haloplanktis are described. protein sequence analysis revealed that the cold-adapted ph dna ligase shows a significant level of sequence similarity to other nad+-dependent dna ligases and contains several previously described sequence motifs. also, a decreased level of arginine and proline residues in ph dna ligase could be involved in the cold-adaptation strateg ...	2000	10848966
culturability and in situ abundance of pelagic bacteria from the north sea.	the culturability of abundant members of the domain bacteria in north sea bacterioplankton was investigated by a combination of various cultivation strategies and cultivation-independent 16s rrna-based techniques. we retrieved 16s rrna gene (rdna) clones from environmental dnas and determined the in situ abundance of different groups and genera by fluorescence in situ hybridization (fish). a culture collection of 145 strains was established by plating on oligotrophic medium. isolates were screen ...	2000	10877804
diversity of thiosulfate-oxidizing bacteria from marine sediments and hydrothermal vents.	species diversity, phylogenetic affiliations, and environmental occurrence patterns of thiosulfate-oxidizing marine bacteria were investigated by using new isolates from serially diluted continental slope and deep-sea abyssal plain sediments collected off the coast of new england and strains cultured previously from galapagos hydrothermal vent samples. the most frequently obtained new isolates, mostly from 10(3)- and 10(4)-fold dilutions of the continental slope sediment, oxidized thiosulfate to ...	2000	10919760
bacterial community structure associated with a dimethylsulfoniopropionate-producing north atlantic algal bloom.	the bacteria associated with oceanic algal blooms are acknowledged to play important roles in carbon, nitrogen, and sulfur cycling, yet little information is available on their identities or phylogenetic affiliations. three culture-independent methods were used to characterize bacteria from a dimethylsulfoniopropionate (dmsp)-producing algal bloom in the north atlantic. group-specific 16s rrna-targeted oligonucleotides, 16s ribosomal dna (rdna) clone libraries, and terminal restriction fragment ...	2000	11010865
search and discovery strategies for biotechnology: the paradigm shift.	profound changes are occurring in the strategies that biotechnology-based industries are deploying in the search for exploitable biology and to discover new products and develop new or improved processes. the advances that have been made in the past decade in areas such as combinatorial chemistry, combinatorial biosynthesis, metabolic pathway engineering, gene shuffling, and directed evolution of proteins have caused some companies to consider withdrawing from natural product screening. in this ...	2000	10974127
cloning, sequences, and characterization of two chitinase genes from the antarctic arthrobacter sp. strain tad20: isolation and partial characterization of the enzymes.	arthrobacter sp. strain tad20, a chitinolytic gram-positive organism, was isolated from the sea bottom along the antarctic ice shell. arthrobacter sp. strain tad20 secretes two major chitinases, chia and chib (archia and archib), in response to chitin induction. a single chromosomal dna fragment containing the genes coding for both chitinases was cloned in escherichia coli. dna sequencing analysis of this fragment revealed two contiguous open reading frames coding for the precursors of archia (8 ...	2001	11160110
cold-adapted beta-galactosidase from the antarctic psychrophile pseudoalteromonas haloplanktis.	the beta-galactosidase from the antarctic gram-negative bacterium pseudoalteromonas haloplanktis tae 79 was purified to homogeneity. the nucleotide sequence and the nh(2)-terminal amino acid sequence of the purified enzyme indicate that the beta-galactosidase subunit is composed of 1,038 amino acids with a calculated m(r) of 118,068. this beta-galactosidase shares structural properties with escherichia coli beta-galactosidase (comparable subunit mass, 51% amino sequence identity, conservation of ...	2001	11282601
phylogenetic diversity of bacterial and archaeal communities in the anoxic zone of the cariaco basin.	microbial community samples were collected from the anoxic zone of the cariaco basin at depths of 320, 500, and 1,310 m on a november 1996 cruise and were used to construct 16s ribosomal dna libraries. of 60 nonchimeric sequences in the 320-m library, 56 belonged to the epsilon subdivision of the proteobacteria (epsilon-proteobacteria) and 53 were closely related to ectosymbionts of rimicaris exoculata and alvinella pompejana, which are referred to here as epsilon symbiont relatives (esr). the 5 ...	2001	11282619
cloning of two pectate lyase genes from the marine antarctic bacterium pseudoalteromonas haloplanktis strain ant/505 and characterization of the enzymes.	a marine antarctic psychrotolerant bacterium (strain ant/505), isolated from sea ice-covered surface water from the southern ocean, showed pectinolytic activity on citrus pectin agar. the sequencing of the 16s rrna of isolate ant/505 indicates a taxonomic affiliation to pseudoalteromonas haloplanktis. the supernatant of this strain showed three different pectinolytic activities after growth on citrus pectin. by activity screening of a genomic dna library of isolate ant/505 in escherichia coli, t ...	2001	11302501
bacteria mediate methylation of iodine in marine and terrestrial environments.	methyl iodide (ch(3)i) plays an important role in the natural iodine cycle and participates in atmospheric ozone destruction. however, the main source of this compound in nature is still unclear. here we report that a wide variety of bacteria including terrestrial and marine bacteria are capable of methylating the environmental level of iodide (0.1 microm). of the strains tested, rhizobium sp. strain mrcd 19 was chosen for further analysis, and it was found that the cell extract catalyzed the me ...	2001	11375186
a novel replication element from an antarctic plasmid as a tool for the expression of proteins at low temperature.	genetic manipulation of antarctic bacteria has been very limited so far. this article reports the isolation and molecular characterization of a novel plasmid, pmtbl, from the antarctic gram-negative bacterium pseudoalteromonas haloplanktis tac 125. this genetic element, 4,081 bp long, appeared to be a multicopy cryptic replicon with no detectable transcriptional activity. by an in vivo assay, the pmtbl autonomous replication sequence was functionally limited to an alui plasmid fragment of about ...	2001	11523895
growth patterns of two marine isolates: adaptations to substrate patchiness?	during bottle incubations of heterotrophic marine picoplankton, some bacterial groups are conspicuously favored. in an earlier investigation bacteria of the genus pseudoalteromonas rapidly multiplied in substrate-amended north sea water, whereas the densities of oceanospirillum changed little (h. eilers, j. pernthaler, and r. amann, appl. environ. microbiol. 66:4634-4640, 2000). we therefore studied the growth patterns of two isolates affiliating with pseudoalteromonas and oceanospirillum in bat ...	2001	11526008
sodium ion cycle in bacterial pathogens: evidence from cross-genome comparisons.	analysis of the bacterial genome sequences shows that many human and animal pathogens encode primary membrane na+ pumps, na+-transporting dicarboxylate decarboxylases or na+ translocating nadh:ubiquinone oxidoreductase, and a number of na+ -dependent permeases. this indicates that these bacteria can utilize na+ as a coupling ion instead of or in addition to the h+ cycle. this capability to use a na+ cycle might be an important virulence factor for such pathogens as vibrio cholerae, neisseria men ...	2001	11528000
structural investigation on the lipooligosaccharide fraction of psychrophilic pseudoalteromonas haloplanktis tac 125 bacterium.	the core structure of the cell-wall lipooligosaccharide (los) fraction of an antarctic gram-negative bacterium, pseudoalteromonas haloplanktis tac 125 strain, was determined to be deacetylated alditols. these were obtained from native los fraction by o-deacylation, dephosphorylation, reduction and finally n-deacylation. two novel structures were detected, the more highly represented molecule consisting of the following hexasaccharide chain: alpha-d-manpnh(2)-(1-->3)-beta-d-galp-(1-->4)-alpha-l-g ...	2001	11589700
the meroperon of a mercury-resistant pseudoalteromonas haloplanktis strain isolated from minamata bay, japan.	a mer operon of mercury-resistant pseudoalteromonas haloplanktis strain m1, isolated from sea water of minamata bay, was cloned and analyzed. the mer genes were located in the chromosome and organized as merr-mert-merp-merc-mera-merd, the same order as that in tn21. however, the orientation of the merr gene is the same as that of other mer genes (opposite direction to tn21), and merr was cotranscribed with other mer genes, a pattern that has not been previously seen with mer determinants from ot ...	2001	11601623
diversity and detection of nitrate assimilation genes in marine bacteria.	a pcr approach was used to construct a database of nasa genes (called narb genes in cyanobacteria) and to detect the genetic potential for heterotrophic bacterial nitrate utilization in marine environments. a nasa-specific pcr primer set that could be used to selectively amplify the nasa gene from heterotrophic bacteria was designed. using seawater dna extracts obtained from microbial communities in the south atlantic bight, the barents sea, and the north pacific gyre, we pcr amplified and seque ...	2001	11679368
cell-cell and cell-surface interactions in an illuminated biofilm: implications for marine sediment stabilization.	most wetted surfaces that are illuminated support a population of phototrophs. the marine sediment is no exception and there the major component of the microphytobenthic population is diatoms. these organisms are credited with stabilizing the sediment against physical disturbance by virtue of the extracellular carbohydrate polymers that they elaborate. however, diatoms synthesize and secrete several carbohydrate polymers and it is not certain which of them is involved in the stabilization proces ...	2001	16759422
structural basis of alpha-amylase activation by chloride.	to further investigate the mechanism and function of allosteric activation by chloride in some alpha-amylases, the structure of the bacterial alpha-amylase from the psychrophilic micro-organism pseudoalteromonas haloplanktis in complex with nitrate has been solved at 2.1 a degrees, as well as the structure of the mutants lys300gln (2.5 a degrees ) and lys300arg (2.25 a degrees ). nitrate binds strongly to alpha-amylase but is a weak activator. mutation of the critical chloride ligand lys300 into ...	2002	12021442
bisucaberin--a dihydroxamate siderophore isolated from vibrio salmonicida, an important pathogen of farmed atlantic salmon (salmo salar).	a siderophore of the bacterial fish pathogen, vibrio salmonicida, was isolated from low-iron culture supernatant and structurally characterized as bisucaberin by fticr- and fab-ms, nmr and gc-ms analysis of the hydrolysis products. although the cyclic dihydroxamate bisucaberin has previously been isolated from a marine bacterium, alteromonas haloplanktis, its involvement in cold-water vibriosis of atlantic salmon (salmon salar) is novel. bisucaberin production in iron-limited media was highest a ...	2002	12046923
a novel family 8 xylanase, functional and physicochemical characterization.	xylanases are generally classified into glycosyl hydrolase families 10 and 11 and are found to frequently have an inverse relationship between their pi and molecular mass values. however, we have isolated a psychrophilic xylanase that belongs to family 8 and which has both a high pi and high molecular mass. this novel xylanase, isolated from the antarctic bacterium pseudoalteromonas haloplanktis, is not homologous to family 10 or 11 enzymes but has 20-30% identity with family 8 members. nmr anal ...	2002	12089151
lipid a structure of pseudoalteromonas haloplanktis tac 125: use of electrospray ionization tandem mass spectrometry for the determination of fatty acid distribution.	the use of electrospray ionization (esi) tandem mass spectrometry (ms/ms) for the structural determination of the lipid a components of the psychrophilic bacterium pseudoalteromonas haloplanktis tac 125 is reported. the lipid a contains the classical bisphosphorylated beta-(1' --> 6)-linked d-glucosamine disaccharide with 3-hydroxydodecanoyl residues (12 : 0 (3-oh)) linked both as esters and amides to 2', 3' (distal glucosamine) and 2, 3 positions (proximal glucosamine) of the sugar backbone. th ...	2002	12112753
crystallization and preliminary x-ray analysis of a xylanase from the psychrophile pseudoalteromonas haloplanktis.	the 46 kda xylanase from the antarctic microorganism pseudoalteromonas haloplanktis is an enzyme that efficiently catalyzes reactions at low temperatures. here, the crystallization of both the native protein and the semet-substituted enzyme and data collection from both crystals using synchrotron radiation are described. the native data showed that the crystals diffract to 1.3 a resolution and belong to space group p2(1)2(1)2(1), with unit-cell parameters a = 50.87, b = 90.51, c = 97.23 a. sad d ...	2002	12198313
a monocarboxylate permease of rhizobium leguminosarum is the first member of a new subfamily of transporters.	amino acid transport by rhizobium leguminosarum is dominated by two abc transporters, the general amino acid permease (aap) and the branched-chain amino acid permease (bra). however, mutation of these transporters does not prevent this organism from utilizing alanine for growth. an r. leguminosarum permease (mctp) has been identified which is required for optimal growth on alanine as a sole carbon and nitrogen source. characterization of mctp confirmed that it transports alanine (k(m) = 0.56 mm) ...	2002	12218032
fish tale.	the draft sequence of the genome of the japanese pufferfish has just been announced and the temptation to humor is great.	2002	12225574
secretion of alpha-amylase from pseudoalteromonas haloplanktis tab23: two different pathways in different hosts.	secretion of cold-adapted alpha-amylase from pseudoalteromonas haloplanktis tab23 was studied in three antarctic bacteria. we demonstrated that the enzyme is specifically secreted in the psychrophilic hosts even in the absence of a protein domain that has been previously reported to be necessary for alpha-amylase secretion in escherichia coli. the occurrence of two different secretion pathways in different hosts is proposed.	2002	12270842
dual effects of an extra disulfide bond on the activity and stability of a cold-adapted alpha-amylase.	chloride-dependent alpha-amylases constitute a well conserved family of enzymes thereby allowing investigation of the characteristics of each member to understand, for example, relevant properties required for environmental adaptation. in this context, we have constructed a double mutant (q58c/a99c) of the cold-active and heat-labile alpha-amylase from the antarctic bacterium pseudoalteromonas haloplanktis, defined on the basis of its strong similarity with the mesophilic enzyme from pig pancrea ...	2002	12324460
identification of dna-synthesizing bacterial cells in coastal north sea plankton.	we describe a method for microscopic identification of dna-synthesizing cells in bacterioplankton samples. after incubation with the halogenated thymidine analogue bromodeoxyuridine (brdu), environmental bacteria were identified by fluorescence in situ hybridization (fish) with horseradish peroxidase (hrp)-linked oligonucleotide probes. tyramide signal amplification was used to preserve the fish staining during the subsequent immunocytochemical detection of brdu incorporation. dna-synthesizing c ...	2002	12406771
antifouling activities expressed by marine surface associated pseudoalteromonas species.	abstract members of the marine bacterial genus pseudoalteromonas have been found in association with living surfaces and are suggested to produce bioactive compounds against settlement of algal spores, invertebrate larvae, bacteria and fungi. to determine the extent by which these antifouling activities and the production of bioactive compounds are distributed amongst the members of the genus pseudoalteromonas, 10 different pseudoalteromonas species mostly derived from different host organisms w ...	2002	19709238
bacterial tracking of motile algae.	abstract we investigated whether bacterial motility and chemotaxis in the ocean enables bacteria to approach and follow microscopic, moving, point sources of nutrients. the turbulent nature of the ocean combined with the imprecision of run and tumble chemotaxis has led to the assumption that marine bacteria cannot cluster around microscopic point sources. recent work, however, shows that marine bacteria use a run and reverse strategy. we examine the ability of marine bacteria that use run and re ...	2003	19719653
marine bacterial organisation around point-like sources of amino acids.	abstract to better understand the trigger for and use of motility in marine bacterial chemotaxis, specific (amino acids) chemical stimuli were used to test the effect on bacterial speed and reorientation. an assay system was developed to analyse bacterial behavioural responses to point-like nutrient sources (beads 10-40 mum). the marine bacteria pseudoalteromonas haloplanktis, shewanella putrefaciens, deleya marina, the enteric bacterium escherichia coli and an enriched assemblage of marine bact ...	2003	19719700
the structure of a cold-adapted family 8 xylanase at 1.3 a resolution. structural adaptations to cold and investgation of the active site.	enzymes from psychrophilic organisms differ from their mesophilic counterparts in having a lower thermostability and a higher specific activity at low and moderate temperatures. the current consensus is that they have an increased flexibility, enhancing accommodation and transformation of the substrates at low energy costs. here we describe the structure of the xylanase from the antarctic bacterium pseudoalteromonas haloplanktis at 1.3 a resolution. xylanases are usually grouped into glycosyl hy ...	2003	12475991
groel from the psychrophilic bacterium pseudoalteromonas haloplanktis tac 125: molecular characterization and gene cloning.	the heat shock response of the psychrophilic bacterium pseudoalteromonas haloplanktis tac 125 (phtac 125) gives rise to the production of several inducible proteins. among these, the protein corresponding to a 55-kda band on sds-page was purified to homogeneity and identified as a groel-like protein. the gene coding for this protein (phgroel) was cloned and sequenced; the deduced amino acid sequence shows 82% sequence identity to groel from escherichia coli (ecgroel). the orf found in the 5' ups ...	2003	12579376
structure and membrane affinity of a suite of amphiphilic siderophores produced by a marine bacterium.	iron concentrations in the ocean are low enough to limit the growth of marine microorganisms, which raises questions about the molecular mechanisms these organisms use to acquire iron. marine bacteria have been shown to produce siderophores to facilitate iron(iii) uptake. we describe the structures of a suite of amphiphilic siderophores, named the amphibactins, which are produced by a nearshore isolate, gamma proteobacterium, vibrio sp. r-10. each amphibactin has the same tris-hydroxamate-contai ...	2003	12651947
the ars detoxification system is advantageous but not required for as(v) respiration by the genetically tractable shewanella species strain ana-3.	arsenate [as(v); haso(4)(2-)] respiration by bacteria is poorly understood at the molecular level largely due to a paucity of genetically tractable organisms with this metabolic capability. we report here the isolation of a new as(v)-respiring strain (ana-3) that is phylogenetically related to members of the genus shewanella and that also provides a useful model system with which to explore the molecular basis of as(v) respiration. this gram-negative strain stoichiometrically couples the oxidati ...	2003	12732551
carriage of potentially fish-pathogenic bacteria in sparus aurata cultured in mediterranean fish farms.	a bacteriological survey of gilthead sea bream sparus aurata from different fish farms and culture systems on the spanish mediterranean coast was conducted. three different studies were performed. study a included hatchery-reared larvae; study b, periodic examination of randomly sampled growing fish; and study c, growing fish sampled only during mortality/morbidity events. in studies b and c, sea cages, earth ponds and indoor tanks were surveyed, and in both cases diseased (showing clinical sign ...	2003	12747637
expression, purification, crystallization and preliminary x-ray crystallographic studies of a psychrophilic cellulase from pseudoalteromonas haloplanktis.	the antarctic psychrophile pseudoalteromonas haloplanktis produces a cold-active cellulase. to date, a three-dimensional structure of a psychrophilic cellulase has been lacking. crystallographic studies of this cold-adapted enzyme have therefore been initiated in order to contribute to the understanding of the molecular basis of the cold adaptation and the high catalytic efficiency of the enzyme at low and moderate temperatures. the catalytic core domain of the psychrophilic cellulase celg from ...	2003	12832777
the precursor of a psychrophilic alpha-amylase: structural characterization and insights into cold adaptation.	the alpha-amylase precursor from the bacterium pseudoalteromonas haloplanktis possesses a propeptide at the c-terminus possibly responsible for outer membrane translocation. unlike the predicted beta-barrel of autotransporters, this c-terminal propeptide displays a noticeable alpha-helix content. it is connected to the enzyme by a disordered linker and has no significant interaction with the catalytic domain. the microcalorimetric pattern of the precursor also demonstrates that the stability of ...	2003	12878029
biochemical characterization of a beta-galactosidase with a low temperature optimum obtained from an antarctic arthrobacter isolate.	a psychrophilic gram-positive isolate was obtained from antarctic dry valley soil. it utilized lactose, had a rod-coccus cycle, and contained lysine as the diamino acid in its cell wall. consistent with these physiological traits, the 16s ribosomal dna sequence showed that it was phylogenetically related to other arthrobacter species. a gene (bgas) encoding a family 2 beta-galactosidase was cloned from this organism into an escherichia coli host. preliminary results showed that the enzyme was co ...	2003	12949099
determination of phosphorylation sites in lipooligosaccharides from pseudoalteromonas haloplanktis tac 125 grown at 15 degrees c and 25 degrees c by nano-electrospray ionization quadrupole time-of-flight tandem mass spectrometry.	lipooligosaccharides (loss) are macromolecules present on the external cellular membrane of gram-negative bacteria, structurally made of two distinct regions, lipid a and core. by varying their growth temperature, bacteria such as psychrophiles change the phosphorylation distribution of the loss produced. the level of phosphorylation and the phosphate group positions in loss produced by the extremophile psychrophilic bacterium pseudoalteromonas haloplanktis tac 125, grown at 15 degrees c and 25 ...	2003	14515321
prospecting for novel biocatalysts in a soil metagenome.	the metagenomes of complex microbial communities are rich sources of novel biocatalysts. we exploited the metagenome of a mixed microbial population for isolation of more than 15 different genes encoding novel biocatalysts by using a combined cultivation and direct cloning strategy. a 16s rrna sequence analysis revealed the presence of hitherto uncultured microbes closely related to the genera pseudomonas, agrobacterium, xanthomonas, microbulbifer, and janthinobacterium. total genomic dna from t ...	2003	14532085
extremophiles 2002.		2003	12813059
unusual microbial xylanases from insect guts.	recombinant dna technologies enable the direct isolation and expression of novel genes from biotopes containing complex consortia of uncultured microorganisms. in this study, genomic libraries were constructed from microbial dna isolated from insect intestinal tracts from the orders isoptera (termites) and lepidoptera (moths). using a targeted functional assay, these environmental dna libraries were screened for genes that encode proteins with xylanase activity. several novel xylanase enzymes wi ...	2004	15184164
influence of growth temperature on lipid and phosphate contents of surface polysaccharides from the antarctic bacterium pseudoalteromonas haloplanktis tac 125.	the chemical structural variations induced by different growth temperatures in the lipooligosaccharide and exopolysaccharide components extracted from the antarctic bacterium pseudoalteromonas haloplanktis tac 125 are described. the increase in phosphorylation with the increase in growth temperature seems to be general, because it happens not only for the lipooligosaccharide but also for the exopolysaccharide. structural variations in the lipid components of lipid a also occur. in addition, free ...	2004	14679221
adaptation to extreme environments: macromolecular dynamics in bacteria compared in vivo by neutron scattering.	mean macromolecular dynamics was quantified in vivo by neutron scattering in psychrophile, mesophile, thermophile and hyperthermophile bacteria. root mean square atomic fluctuation amplitudes determining macromolecular flexibility were found to be similar for each organism at its physiological temperature ( approximately 1 a in the 0.1 ns timescale). effective force constants determining the mean macromolecular resilience were found to increase with physiological temperature from 0.2 n/m for the ...	2004	14710189
adaptation to extreme environments: macromolecular dynamics in bacteria compared in vivo by neutron scattering.	mean macromolecular dynamics was quantified in vivo by neutron scattering in psychrophile, mesophile, thermophile and hyperthermophile bacteria. root mean square atomic fluctuation amplitudes determining macromolecular flexibility were found to be similar for each organism at its physiological temperature ( approximately 1 a in the 0.1 ns timescale). effective force constants determining the mean macromolecular resilience were found to increase with physiological temperature from 0.2 n/m for the ...	2004	14710189
promoters from a cold-adapted bacterium: definition of a consensus motif and molecular characterization of up regulative elements.	although low-temperature tolerant micro-organisms were discovered long ago, limited information is still available on the transcription machinery in cold-adapted bacteria. this knowledge represents a necessary background for the investigation of the adaptation of gene-expression mechanisms at low temperatures. the recent development of a shuttle genetic system for the transformation of the cold-adapted gram-negative bacterium pseudoalteromonas haloplanktis strain tac125 has made possible the iso ...	2004	15064979
antibiotic production by a roseobacter clade-affiliated species from the german wadden sea and its antagonistic effects on indigenous isolates.	a strain affiliated with the roseobacter clade and producing a new antibiotic named tropodithietic acid (l. liang, ph.d. thesis, university of göttingen, göttingen, germany, 2003) was isolated from the german wadden sea. the compound showed strong inhibiting properties with respect to marine bacteria of various taxa and marine algae. antibiotic production was found to occur during the complete growth phase. strain mutants without antagonistic properties appeared several times spontaneously.	2004	15066861
recombinant protein production in antarctic gram-negative bacteria.	this review reports some results from our laboratory on the setting up of a psychrophilic expression system for the homologous/heterologous protein production in cold-adapted bacteria by using natural plasmids as cloning vectors. by screening some antarctic bacteria for the presence of extrachromosomal elements, we identified three new plasmids, pmtbl from pseudoalteromonas haloplanktis tac125, and ptaup and ptadw, from psychrobacter sp. ta144. the latter autoreplicating elements were isolated, ...	2004	15269427
kinetic and structural optimization to catalysis at low temperatures in a psychrophilic cellulase from the antarctic bacterium pseudoalteromonas haloplanktis.	the cold-adapted cellulase celg has been purified from the culture supernatant of the antarctic bacterium pseudoalteromonas haloplanktis and the gene coding for this enzyme has been cloned, sequenced and expressed in escherichia coli. this cellulase is composed of three structurally and functionally distinct regions: an n-terminal catalytic domain belonging to glycosidase family 5 and a c-terminal cellulose-binding domain belonging to carbohydrate-binding module family 5. the linker of 107 resid ...	2004	15287848
expression of a temperature-sensitive esterase in a novel chaperone-based escherichia coli strain.	a new principle for expression of heat-sensitive recombinant proteins in escherichia coli at temperatures close to 4 degrees c was experimentally evaluated. this principle was based on simultaneous expression of the target protein with chaperones (cpn60 and cpn10) from a psychrophilic bacterium, oleispira antarctica rb8(t), that allow e. coli to grow at high rates at 4 degrees c (maximum growth rate, 0.28 h(-1)). the expression of a temperature-sensitive esterase in this host at 4 to 10 degrees ...	2004	15294778
[elucidation of structure of lipid a from the marine gram-negative bacterium pseudoalteromonas haloplanktis atcc 14393t].	the chemical structure of lipid a from the marine gamma-proteobacterium pseudoalteromonas haloplanktis atcc 14393t, a main product of lipopolysaccharide hydrolysis (1% acoh), was determined using chemical methods and nmr spectroscopy. the lipid a was shown to be beta-1,6-glucosaminobiose 1,4'-diphosphate acylated with two (r)-3-hydroxyalkanoic acid residues at c3 and c3' and amidated with one (r)-3-hydroxydodecanoyl and one (r)-3-dodecanoyloxydodecanoyl residue at n2 and n2', respectively.	2004	15469016
elongation factor ts from the antarctic eubacterium pseudoalteromonas haloplanktis tac 125: biochemical characterization and cloning of the encoding gene.	the elongation factor ts was isolated from the psychrophilic antarctic eubacterium pseudoalteromonas haloplanktis tac 125 strain (phef-ts), and its functional properties were studied. at 0 degrees c phef-ts enhanced the [(3)h]gdp/gdp exchange rate on the preformed phef-tu.[(3)h]gdp complex by 2 orders of magnitude even at very low tu:ts ratio, by lowering the energy of activation of the exchange reaction. phef-ts is a monomeric protein, and in solution it forms a stable dimeric complex with phef ...	2004	15544346
[characterization and gene cloning of the endoglucanase from pseudoalteromonas sp. dy3 strain].	a bacteria strain dy3 with high endoglucanse activity was isolated from deep sea sediment sample es0109. the 16s rdna sequence of dy3 exhibits identity of 99% with those of the same genus bacteria pseudoalteromonas citrea and pseudoalteromonas elyakovii . the celx gene of dy3 obtained by pcr method is 1479bp in length and encodes a protein of 492 amino acids. the protein encoded by celx gene exhibits 95% sequence identity with endoglucanase celg from pseudoalteromonas haloplanktis. there are two ...	2004	15969114