Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| enzymatic characterization of some oral and nonoral gram-negative bacteria with the api zym system. | the api zym system (analytab products, plainview, n.y.), containing 19 chromogenic substrates, was utilized semiquantitatively to detect extracellular acid and alkaline phosphatases, aminopeptidases, proteases, esterase-lipase, phosphoamidase, and glycosidases in 128 oral and nonoral isolates of black-pigmented bacteroides, actinobacillus, haemophilus aphrophilus, capnocytophaga, fusobacterium nucleatum, wolinella recta, and veillonella parvula. in the black-pigmented bacteroides group of organi ... | 1981 | 7026598 |
| similarity of wolinella recta strains isolated from periodontal pockets and root canals. | 1982 | 6955433 | |
| comparison of strains of gram-negative, anaerobic, agar-corroding rods isolated from soft tissue infections in cats and dogs with type strains of bacteroides gracilis, wolinella recta, wolinella succinogenes, and campylobacter concisus. | a total of 64 strains of gram-negative, asaccharolytic, anaerobic, agar-corroding, rod-shaped bacteria from soft-tissue infections of cats and dogs were compared with other agar-corroding, anaerobic organisms isolated from human periodontal pockets (wolinella recta atcc 33238t), bovine rumens (wolinella succinogenes atcc 29543t), and gingival crevices of humans (bacteroides gracilis atcc 33236t and bacteroides ureolyticus nctc 10941t). campylobacter concisus atcc 33237t (from human gingival crev ... | 1984 | 6490858 |
| use of isoelectric focusing of bacterial proteins for characterisation of strains of motile anaerobic "corroding" bacteria isolated from soft tissue infections in cats and dogs. | isoelectric focusing of bacterial proteins in 1 mm thick agarose gels and ultrastructural analysis of cell walls were used to facilitate characterisation of motile "corroding" bacteria from subcutaneous abscesses and pyothorax infections from cats and dogs. some of the motile "corroding" rods had ultrastructural cell wall features identical with wolinella recta described from human periodontal pockets. other strains had the more conventional cell wall ultrastructure of gram negative bacteria. is ... | 1984 | 6499406 |
| isolation of wolinella recta and actinomyces viscosus from an actinomycotic chest wall mass. | in this note we describe a patient with an actinomycotic chest wall mass from which large numbers of wolinella recta and actinomyces viscosus organisms were isolated. this is the first reported association of w. recta with extraoral infection and the tenth report of lung infection by a. viscosus. | 1984 | 6520225 |
| osteomyelitis in dogs and cats caused by anaerobic bacteria. | localised osteomyelitis was diagnosed in 2 dogs and 2 cats. the disease was caused by fight wounds in 3 cases. radiographic examination demonstrated a circumscribed zone of cortical bone lysis, sequestra and periosteal new bone. each case was treated surgically by sequestrectomy and debridement. infection was due mainly to anaerobic bacteria. the pathogenic bacteria isolated from the lesions of dogs were actinomyces viscosus, fusobacterium nucleatum and bacteroides spp, and from the lesions in c ... | 1984 | 6732670 |
| gram negative species associated with active destructive periodontal lesions. | apical subgingival plaque samples were taken from 19 subjects exhibiting active destructive periodontal disease. the predominant cultivable gram negative species from 50 active sites were compared to 69 inactive sites of comparable pocket depth and attachment level loss. active disease sites were chosen which showed a significant loss of attachment within a two-month interval. proportions of gram negative rods were higher in active periodontal disease sites than in inactive sites. species which ... | 1985 | 3863838 |
| the relationships between streptococcal species and periodontopathic bacteria in human dental plaque. | the existence of antagonistic and commensal relationships between microorganisms was investigated. the predominant cultivable flora in 172 plaque samples from active and non-active sites in 32 human subjects with destructive periodontitis was determined. the presence of putative periodontopathic organisms (bacteroides gingivalis, bacteroides intermedius, bacteroides forsythus, wolinella recta, actinobacillus actinomycetemcomitans, and eikenella corrodens) in a site was correlated with the absenc ... | 1985 | 3868968 |
| api zym and api an-ident reactions of fastidious oral gram-negative species. | api zym and api an-ident enzymatic substrate tests were done on six oral species which are difficult to characterize with conventional biochemical tests. "bacteroides forsythus, the "fusiform" bacteroides species (a. c. r. tanner, m. a. listgarten, m. n. strzempko, and j. l. ebersole, manuscript in preparation), is difficult to cultivate in broth media, yet it gave 15 positive tests in these series. the tests were able to separate this new species from species of capnocytophaga and fusobacterium ... | 1985 | 3930558 |
| chemotactic response to formate by campylobacter concisus and its potential role in gingival colonization. | cells of campylobacter concisus 288 were chemotactic toward formate, but not to any other compound tested. chemicals that were not chemoattractants included 20 sugars, inorganic salts, amino acids, and their derivatives, purines and pyrimidines, fatty acids, and natural mixtures such as saliva, serum, crevicular fluid, and mucin. chemotaxis was measured quantitatively by a modification of the capillary method. cells were suspended in 0.01 m tris buffer, ph 7.5, supplemented with 5 mm kcl, 0.1% n ... | 1986 | 3699887 |
| bacteroides buccae and related taxa in necrotic root canal infections. | fifty-seven adults with apical periodontitis were examined for the presence of nonpigmented bacteroides species in 62 infected root canals. nonpigmented bacteroides species were found in 35 canals. in four cases two nonpigmented bacteroides species and in one case three nonpigmented bacteroides species were found. species belonging to the b. fragilis group were not isolated. the most frequently isolated species were b. buccae (15 strains), b. oris (12 strains), and b. oralis (7 strains). alpha-f ... | 1986 | 3782459 |
| nutritional relationships between oral bacteria. | nutritional relationships were revealed during the coculturing of bacteroides gingivalis with wolinella recta and bacteroides melaninogenicus with w. recta. w. recta produced a substance that stimulated the growth of b. gingivalis and b. melaninogenicus. characterization by thin-layer chromatography and absorption spectrometry identified the compound as protoheme. production of large amounts of formate by b. melaninogenicus stimulated the growth of w. recta. these nutritional relationships could ... | 1986 | 2875029 |
| wolinella recta, campylobacter concisus, bacteroides gracilis, and eikenella corrodens from periodontal lesions. | 1987 | 2957486 | |
| growth studies of wolinella recta, a gram-negative periodontopathogen. | 1987 | 3507619 | |
| human serum antibody responses to oral microorganisms. iv. correlation with homologous infection. | recent microbiological studies of periodontal disease in humans have supported the concept of a specific bacterial etiology. while individual agents have not been unequivocally identified, numerous gram-negative members of the subgingival microflora have been implicated. in addition, elevations in systemic antibody responses have been consistent with certain oral microorganisms being involved in an infectious process associated with the disease. this report delineates the relationship between el ... | 1987 | 10870468 |
| lipopolysaccharide-stimulated pge2 release from human monocytes. comparison of lipopolysaccharides prepared from suspected periodontal pathogens. | lipopolysaccharides (lps) prepared from the suspected periodontal pathogens actinobacillus actinomycetemcomitans (a. a.), bacteroides gingivalis, b. intermedius and wolinella recta were compared to salmonella typhimurium lps for their capacity to stimulate prostaglandin e2 (pge2) release from human monocytes. counterflow isolated monocytes were cultured with control medium or media containing 10 micrograms/ml lps. media were then exchanged every 24 hours for a total of 72 hours. salmonella and w ... | 1988 | 3054046 |
| production and characterization of monoclonal antibodies against bacteroides forsythus and wolinella recta. | hybrid cell lines producing monoclonal antibodies against bacteroides forsythus or wolinella recta were generated by fusion of sp2/0 or fo myeloma cells with splenocytes from balb/c mice immunized with formalinized cells of b. forsythus fdc 331 or w. recta d13a-g, respectively. enzyme-linked immunosorbent assays and indirect immunofluorescence tests were used to analyze the distribution of the recognized antigens on a panel of 70 strains representing 35 taxa, most of which are members of the ora ... | 1988 | 3170894 |
| the comparative cytotoxicity of periodontal bacteria. | the direct cytotoxicity of sonic extracts (se) from nine periodontal bacteria for human gingival fibroblasts (hgf) was compared. equivalent dosages (in terms of protein concentration) of se were used to challenge hgf cultures. the cytotoxic potential of each se was assessed by its ability to (1) inhibit hgf proliferation, as measured by direct cell counts; (2) inhibit 3h-thymidine incorporation in hgf cultures; or (3) cause morphological alterations of the cells in challenged cultures. the highe ... | 1988 | 3204481 |
| chemical and biological characterization of the lipopolysaccharide of the oral pathogen wolinella recta atcc 33238. | to investigate the potential pathogenic mechanisms of the oral periodontopathogen wolinella recta atcc 33238, we have isolated its lipopolysaccharide (lps) and determined the chemical composition and selected in vitro biological activities of the molecule. sodium desoxycholate-polyacrylamide gel electrophoresis revealed the w. recta lps to be an atypical smooth lps with short o-antigenic side chains. chemically the lps consisted of 47.2% lipid a, 19.6% polysaccharide, 9.0% heptose, 8.5% hexosami ... | 1988 | 3260893 |
| microbiological and immunological studies of adult periodontitis in patients with noninsulin-dependent diabetes mellitus. | the subgingival microflora and serum antibody response was examined in periodontitis patients with noninsulin-dependent diabetes mellitus (niddm), impaired glucose tolerance (igt), and normal glucose tolerance (ngt). the predominant cultivable microflora was determined for subgingival plaque sampled from two deep periodontal pockets in each of eight adult periodontitis patients with niddm. indirect immunofluorescence for bacteroides intermedius, bacteroides gingivalis, and haemophilus actinomyce ... | 1988 | 3276868 |
| ultrastructural relationship of cell envelope layers in wolinella recta. | the cell envelope of wolinella recta was studied by electron microscopy. in thin sections the surface layer (s-layer) seemed to be in close contact to the outer membrane (om). a hexagonally arranged pattern of the s-layer was revealed by freeze-etching. unlike in other oral bacteria with an s-layer, the cleavage occurred along the s-layer by freeze-fracture, suggesting an exceptionally tight relationship between the two outermost layers. the convex surface of the s-layer was usually revealed by ... | 1989 | 2469124 |
| dna-dna hybridization incompatibility of campylobacter pylori with other campylobacter and wolinella species. | dna-dna hybridization in solution was used to characterize 23 human isolates of campylobacter pylori. the 23 isolates showed dna affinity with the type strain (nctc 11637). the relative binding ratios varied between 0.83 and 1. type strains of c. coli (nctc 11366), c. jejuni (nctc 11351), c. laridis (nctc 11352), c. sputorum subsp. sputorum (atcc 35980), wolinella recta (nctc 11489) and w. succinogenes (atcc 29543) showed relative binding ratios less than 0.01 compared to the c. pylori type stra ... | 1989 | 2499346 |
| chemical and biological properties of lipopolysaccharide, lipid a and degraded polysaccharide from wolinella recta atcc 33238. | lipopolysaccharide (lps) was isolated and purified from wolinella recta atcc 33238 by the phenol-water procedure and rnaase treatment. the sugar components of the lps were rhamnose, mannose, glucose, heptose, 2-keto-3-deoxyoctonate (kdo) (3-deoxy-d-manno-octulosonate) and glucosamine. the degraded polysaccharide prepared from lps by mild acid hydrolysis was fractionated by sephadex g-50 gel chromatography into three fractions: (1) a high-molecular-mass fraction, eluting just behind the void volu ... | 1989 | 2600585 |
| newly delineated periodontal pathogens with special reference to selenomonas species. | many new species have been isolated from subgingival periodontal pockets, for example wolinella recta and bacteroides forsythus, reflecting better basic microbiological techniques. other species were created from existing species as a result of better characterization methods, i.e. bacteroides buccae and bacteroides oris. we can recognize different types of periodontal disease and can find differences relating to the progressive compared to inactive lesions. data illustrated in this presentation ... | 1989 | 2661440 |
| wolinella recta and spirochetes in relation to alteration of probing depth in a population without regular dental care. | the purpose of this study was to determine the occurence of spirochetes and wolinella sp. in untreated, nonselected subjects and to relate the presence of these organisms to changes in probing depth over a period of 180 days. clinical parameters were recorded and subgingival microbial samples were taken mesiobuccally on the upper first molars of 120 subjects (mean age 37 +/- 14 years) with a history of no regular dental care. the number of motile rods and spirochetes were determined in the darkf ... | 1989 | 2678458 |
| [microbial flora of periodontitis and monitoring of the effect of initial preparation by immunofluorescence microscopy]. | the effect of initial preparation in adult periodontitis was evaluated by changes in clinical parameters and immunofluorescence microscopic counts of periodontal disease associated bacteria. subgingival plaque samples were taken with sterilized paper points from 10 sites of 5 periodontally healthy persons and 44 sites of 23 adult periodontitis patients. twenty-one diseased sites were periodically examined after plaque control and scaling, root planing. the direct immunofluorescence technique was ... | 1989 | 2700361 |
| subgingival microflora, dental and periodontal conditions in patients with hereditary fructose intolerance. | hereditary fructose intolerance (hfi) is a rare autosomally recessive disease which leads to severe hypoglycemia. the fructose-free diet of these patients apparently influences dental health. half of the patients are free of caries, but there are no reports on their periodontal condition nor on the composition of their subgingival microflora. therefore 18 patient with hfi were examined for the following parameters: radiographic bone loss, caries rate, gingival bleeding, occurrence of calculus, p ... | 1989 | 2704977 |
| campylobacter-wolinella group organisms are the only oral bacteria that form arylsulfatase-active colonies on a synthetic indicator medium. | most oral bacteria tested formed colonies on a chemically defined medium with a chromogenic arylsulfatase substrate. arylsulfatase activity was, however, restricted to campylobacter-wolinella group organisms, including wolinella recta, a possible periodontopathogen. w. recta was the only arylsulfatase-active species against which consistently high levels of antibody were detected in human sera. | 1989 | 2707850 |
| a comparison of bacteroides ureolyticus isolates from different clinical sources. | clinical isolates of corroding, gram-negative, anaerobic bacilli (provisionally identified as bacteroides ureolyticus) from superficial ulcers and soft tissue infections (15), non-gonococcal, non-chlamydial urethritis (12) and adult periodontal disease (14) were compared with reference strains of b. ureolyticus, b. gracilis and wolinella recta in a series of conventional tests of morphology, biochemical activity, tolerance of dyes and bile salts, and antibiotic sensitivity, gas-liquid chromatogr ... | 1989 | 2724326 |
| cell surface protein antigen from wolinella recta atcc 33238t. | a high-molecular-weight (approximately 150,000) protein was selectively isolated by acid extraction from the cell surface of wolinella recta and purified by negative adsorption on deae-cellulose and gel filtration. sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that this protein was found in w. recta but not in other wolinella species, such as w. curva and w. succinogenes. sera from patients with periodontitis reacted strongly with this protein antigen, whereas sera fr ... | 1989 | 2753998 |
| the microbiota of early periodontitis lesions in adults. | the predominant cultivable microbiota of shallow progressive (active) periodontal lesions in 4 adults (29-40 years) was compared to that of shallow inactive sites. periodontal attachment levels were monitored longitudinally, and sites showing 2-3 mm attachment loss in the previous 1-2 months were considered disease active. inactive sites showed no change in attachment level. the microbiota of shallow sites was dominated by gram negative rod species including wolinella recta, bacteroides forsythu ... | 1989 | 2768541 |
| the relationship of serum igg antibody titers to periodontal pathogens to indicators of the host response in crevicular fluid. | in this study; the relationship of indicators of the local host response in gingival crevicular fluid (gcf) to the serum antibody titer to periodontal pathogens was examined. 15 patients with chronic adult periodontitis were studied. gcf was collected and analyzed for the total amount of igg, igm, the lysosomal enzyme b-glucuronidase (bg) and alpha-2-macroglobulin (alpha 2m). at the same examination, serum from these patients was collected, and enzyme-linked immunosorbent assays used to determin ... | 1990 | 1696949 |
| three-dimensional structure of the surface layer of wolinella recta. | the three-dimensional structure of the crystalline surface layer (s-layer) of wolinella recta atcc 33238t, a gram-negative, anaerobic periodontopathogen, was determined to 3.8 nm resolution by electron microscopy and digital image processing. the s-layer protein is closely associated with the outer bacterial membrane, and shows p6 symmetry with lattice spacing and thickness of 21 nm and 15 nm, respectively. the funnel-shaped subunits consist of 6 heavy domains located round a common base at the ... | 1990 | 2080071 |
| effect of a triclosan/copolymer/fluoride dentifrice on the oral microflora. | eighty-one human subjects completed a double-blind study which examined the effects of a 0.3% triclosan/2% gantrez copolymer/0.243% sodium fluoride dentifrice on the microflora of supragingival dental plaque. subjects were divided into an experimental group which performed normal oral hygiene with the triclosan/copolymer/fluoride dentifrice and a control group which also performed normal oral hygiene with the same dentifrice minus the triclosan/copolymer. at baseline, 10 weeks, and 28 weeks, sup ... | 1990 | 2083042 |
| monoclonal antibodies for the detection of 'periodontopathic' bacteria. | the development of indirect immunofluorescence assays using mabs as specific probes has made it possible to detect a variety of suspected periodontal pathogens in subgingival plaque, with exquisite sensitivity. the studies demonstrate high reproducibility of the results if different mabs are used to assess the same bacterial species. bacteroides forsythus, bact. gingivalis, bact. intermedius and wolinella recta, but not actinobacillus actinomycetemcomitans were found in sites of adult periodonti ... | 1990 | 2088218 |
| hydrophobicities of human polymorphonuclear leukocytes and oral bacteroides and porphyromonas spp., wolinella recta, and eubacterium yurii with special reference to bacterial surface structures. | the hydrophobicities of human polymorphonuclear leukocytes (pmnls) and bacteroides buccae, b. oris, b. oralis, b. veroralis, b. buccalis, b. heparinolyticus, b. intermedius, b. denticola, b. loescheii, b. melaninogenicus, porphyromonas gingivalis, p. endodontalis, wolinella recta, and eubacterium yurii were studied by the hexadecane method. the majority of the strains were equally or less hydrophobic than the pmnls. only in the case of e. yurii and the only strain of b. buccalis were all strains ... | 1990 | 2091243 |
| effect of actinobacillus actinomycetemcomitans, wolinella recta and bacteroides gingivalis on the viability of retinoic acid-induced and dimethyl sulfoxide-induced hl-60 cells. | we studied the interactions between viable and heat-killed, opsonized and unopsonized periodontopathic bacteria with both uninduced and induced hl-60 promyelocytic leukemia cells. the cells were induced to differentiate into granulocyte-like cells by incubation with retinoic acid (ra) or dimethyl sulfoxide (dmso). when unopsonized, wolinella recta atcc 33228 significantly suppressed the net proliferation of uninduced hl-60 cells, actinobacillus actinomycetemcomitans strain y4 was markedly lethal ... | 1990 | 2098697 |
| interaction of gram-negative periodontal pathogens with retinoic acid-induced and dimethyl sulfoxide-induced hl-60 cells. | as a first step toward elucidating the reasons for differences among periodontal pathogens in their cytotoxic effects on hl-60 cells, we used transmission electron microscopy to examine morphological aspects of granulocyte-bacteria interactions. unopsonized actinobacillus actinomycetemcomitans strain y4 and bacteroides gingivalis atcc 33277 adhered to, and were phagocytosed by, retinoic acid-induced and dimethyl sulfoxide-induced hl-60 cells. in contrast, there was only minimal interaction betwe ... | 1990 | 2098698 |
| sialidase (neuraminidase) activity among gram-negative anaerobic and capnophilic bacteria. | a filter paper spot test with 2'-(4-methylumbelliferyl)-alpha-d-n-acetylneuraminic acid as a substrate was used to study the prevalence of sialidase activity among gram-negative anaerobic and capnophilic bacteria. a total of 567 isolates representing four genera of obligate anaerobes and four genera of capnophilic organisms was tested. sialidase activity was detected in 94% of 66 isolates from the bacteroides fragilis group, 98% of 66 b. bivius isolates, and all isolates of the following species ... | 1990 | 2108991 |
| studies of the subgingival microflora in patients with acquired immunodeficiency syndrome. | two unique forms of periodontal disease, hiv-gingivitis and hiv-periodontitis, have been described in patients with acquired immunodeficiency syndrome (aids). in order to determine the bacterial species associated with periodontitis in aids patients, the predominant cultivable microflora was examined in 21 subgingival plaque samples from 11 aids patients with periodontitis. the presence of putative periodontal pathogens including actinobacillus actinomycetemcomitans, bacteroides intermedius, por ... | 1990 | 2123926 |
| susceptibility of oral bacteria to phenoxyethanol and phenoxyethanol/chlorhexidine combinations. | a total of 57 bacterial strains (26 different species) which may be isolated from subgingival plaque were tested for their in vitro susceptibility to phenoxycthanol, a commonly-used antiseptic and preservative. ninety-five percent of the strains, including those associated with chronic inflammatory periodontal disease, were susceptible to concentrations of phenoxyethanol used topically (2% w/v). phenoxyethanol at a concentration of 1% (w/v) was also found that to have a rapid bactericidal effect ... | 1990 | 2202809 |
| plaque and chronic inflammatory periodontal disease. a question of ecology. | the nature of the relationship between dental plaque and chronic inflammatory periodontal disease (cipd) remains unclear, although there is no doubt that plaque is the direct cause. non-specific, specific and exogenous hypotheses have been proposed to explain plaque-host relationships. current evidence indicates that plaque is part of the natural human microflora, one of many such in nature, and that disruption of oral microbial ecology, due primarily to diet texture changes, leads to gingivitis ... | 1990 | 2212083 |
| associations between six dna probe-detected periodontal bacteria and alveolar bone loss and other clinical signs of periodontitis. | the purpose of the present study was to assess the associations between the presence and amounts of actinobacillus actinomycetemcomitans, bacteroides gingivalis, b. intermedius, eikenella corrodens, wolinella recta, and fusobacterium nucleatum in the periodontal pocket and the degree of alveolar bone loss and other clinical signs of periodonitis, such as probing pocket depth, attachment level, and presence of bleeding on probing at the same site. the study material comprised 16 subjects with or ... | 1990 | 2288212 |
| species-specific oligodeoxynucleotide probes for the identification of periodontal bacteria. | oligodeoxynucleotide probes were developed for identification of the periodontal bacteria actinobacillus actinomycetemcomitans, bacteroides gingivalis, b. intermedius types i and ii, b. forsythus, eikenella corrodens, fusobacterium nucleatum, haemophilus aphrophilus, streptococcus intermedius, and wolinella recta. probes were designed by sequencing the 16s rrna for each bacterium, identifying hypervariable regions, and chemically synthesizing species-specific probes. these probes were specific w ... | 1990 | 2312676 |
| surface characteristics of wolinella recta atcc 33238 and human clinical isolates: correlation of structure with function. | selected characteristics of the surface of wolinella recta atcc 33238 and three w. recta clinical isolates (ci) were studied as well as the adherence of these strains to human gingival fibroblasts (hgf). w. recta atcc 33238 and the ci were examined by electron microscopy, electrophoresis, isoelectric focusing, and adherence to hgf. electron microscopic examination of ci revealed the presence of a periodic paracrystalline layer external to and associated with the outer membrane. this surface laye ... | 1990 | 2387623 |
| antimicrobial effect of chlorhexidine in a controlled release delivery system. | chlorhexidine is widely used as a mouth rinse in the prevention and treatment of periodontal diseases and dental caries. the purpose of the present study was to evaluate the in vitro antimicrobial effect of chlorhexidine in a controlled release delivery system. the controlled release dispenser comprised a polymeric inner core matrix containing the medicament with an outer vinyl membrane controlling the drug release. the effect on the following bacteria was studied: actinobacillus actinomycetemco ... | 1990 | 2390965 |
| brain abscess due to wolinella recta and streptococcus intermedius. | the authors present a case of a 62-year-old female with a frontal lobe brain abscess caused by wolinella recta and two strains of streptococcus intermedius. one of the strains of s intermedius was resistant to penicillin. resolution of the abscess required 60 days of antibiotic therapy and aspiration on four occasions. the source of the brain abscess could not be determined. this is the first case in which w recta has been isolated from a brain abscess. | 1990 | 22553434 |
| revision of campylobacter, helicobacter, and wolinella taxonomy: emendation of generic descriptions and proposal of arcobacter gen. nov. | hybridization experiments were carried out between dnas from more than 70 strains of campylobacter spp. and related taxa and either 3h-labeled 23s rrnas from reference strains belonging to campylobacter fetus, campylobacter concisus, campylobacter sputorum, campylobacter coli, and campylobacter nitrofigilis, an unnamed campylobacter sp. strain, and a wolinella succinogenes strain or 3h- or 14c-labeled 23s rrnas from 13 gram-negative reference strains. an immunotyping analysis of 130 antigens ver ... | 1991 | 1704793 |
| atypical structure of the 23s ribosomal rna molecule in certain oral bacteria. | ribosomal rna (rrna) isolated from wolinella recta and seven related bacteria was examined by agarose gel electrophoresis. the 23s rrna molecule could not be detected in w. recta, wolinella curva, bacteroides gracilis, or bacteroides ureolyticus. in place of the 23s molecule, there were three smaller molecules of approximately 1700, 650, and 600 bases designated 23s alpha, 23s beta, and 23s delta, respectively. an intact 23s rrna molecule could be isolated from wolinella succinogenes, campylobac ... | 1991 | 1710629 |
| the microflora of periodontal sites showing active destructive progression. | 20 adult periodontitis (ap) subjects were examined every 2 to 4 months and microbiological samples were collected and cultured when 2 mm or more loss of attachment (active sites) was detected by 2 examiners. similar sites in which no progressive destruction was observed (control sites) also were sampled in the same subjects. by lambda-analysis, there was no statistically significant difference in floras of active (42 sites from 12 subjects) and control (36 sites from 12 subjects) sites or betwee ... | 1991 | 1752997 |
| antimicrobial activity of flurbiprofen and ibuprofen in vitro against six common periodontal pathogens. | nonsteroidal antiinflammatory drugs (nsaids) such as flurbiprofen and ibuprofen, have been shown to inhibit the inflammation and alveolar bone loss associated with chronic destructive periodontal disease. however, the direct effect of nsaids on the gingival crevice microflora has not been studied. the purpose of this investigation was to evaluate the antimicrobial activity of ibuprofen and flurbiprofen in vitro on six commonly isolated periodontal pathogens. the bacterial strains evaluated were ... | 1991 | 1812907 |
| multicenter evaluation of tetracycline fiber therapy. iii. microbiological response. | in a multicenter study of the effects of tetracycline (tc) fiber therapy, subgingival plaque samples were tested for 6 probable periodontal pathogens by dna probe analysis. levels of actinobacillus actinomycetemcomitans, eikenella corrodens, fusobacterium nucleatum, porphyromonas (bacteroides) gingivalis, prevotella intermedia (bacteroides intermedius), and wolinella recta were quantitatively determined in samples taken at baseline, and immediately after tc fiber removal, control fiber removal, ... | 1991 | 1832457 |
| tetracycline fiber therapy monitored by dna probe and cultural methods. | oligonucleotide dna probe and selective cultural methods were compared in their ability to monitor 6 putative periodontal pathogens in a study evaluating local tetracycline fiber therapy. subgingival plaque was sampled from 4 sites in each of 20 subjects. samples were taken before and after therapy from sites assigned to the following test groups: tetracycline (tc) fiber, scaling and root planing, control fiber, and untreated. each sample was analyzed by both dna probe and cultural methods. tota ... | 1991 | 1832458 |
| wolinella recta, wolinella curva, bacteroides ureolyticus, and bacteroides gracilis are microaerophiles, not anaerobes. | although the nonfermentative, asaccharolytic, putative anaerobes wolinella curva, wolinella recta, bacteroides ureolyticus, and bacteroides gracilis are phylogenetically related to the true campylobacters, the type strains of these species exhibited o2-dependent microaerophilic growth in brucella broth and on brucella agar. the optimum o2 levels for growth of these strains ranged from 4 to 14% in brucella broth and from 2 to 8% on brucella agar, when h2 was provided as the electron donor. no gro ... | 1991 | 1854636 |
| virulence of wolinella recta in a murine abscess model. | the virulence of wolinella recta isolates was studied in an experimental animal model by using monoinfection of balb/c mice. infection with clinical isolates of w. recta 576 and w. recta 234 induced dry, flat, depressed gangrenous necrotic skin lesions, whereas w. recta atcc 33238 failed to induce a similar lesion. histological examination of the skin lesion 72 h postinfection revealed coagulation necrosis of the epidermis, subcutis and cutaneous truncus muscle, with marked exudation of serum pr ... | 1991 | 1855996 |
| dental bacterial plaques. nature and role in periodontal disease. | antony van leeuwenhoek first described oral bacteria. however, not until almost 200 years later was the famous koch postulate introduced. since then, research has extensively been performed regarding the development and microbiology of dental plaques. in spite of the complexity of the developing flora of supragingival plaque, culture studies have shown a remarkably orderly succession of organisms. lately, the concept of microbial specificity in the etiology of periodontal diseases has been widel ... | 1991 | 1890226 |
| extraction, purification, and characterization of major outer membrane proteins from wolinella recta atcc 33238. | the outer membrane of wolinella recta atcc 33238 was isolated by french pressure cell disruption and differential centrifugation. outer membrane proteins (omps) were solubilized by zwittergent 3.14 extraction and separated by deae-sephacel ion-exchange chromatography. the major omps that were found in w. recta atcc 33238 and in several other wolinella spp. consisted of proteins with apparent molecular masses of 51, 45, and 43 kda. these three conserved proteins were purified to essential homogen ... | 1991 | 1894372 |
| microbial aspects of failing osseointegrated dental implants in humans. | the subgingival microflora of 18 failing implants were examined for pathogenic periodontal microorganisms. peptostreptococcus micros was recovered from 6 failing implants, wolinella recta from 6, fusobacterium species from 5, candida albicans from 5, and bacteroides intermedius from 4. enteric rods or pseudomonads constituted a significant part of the microflora in 5 failing implants. actinobacillus actinomycetemcomitans, nonpigmented bacteroides species, capnocytophage species, and staphylococc ... | 1991 | 1906537 |
| comparative study of lipopolysaccharides from wolinella recta, w. curva, w. succinogenes and campylobacter sputorum ssp. sputorum. | lipopolysaccharides (lps) were extracted from cells of wolinella recta atcc 33238, w. curva atcc 33224, w. succinogenes atcc 29543 and campylobacter sputorum ssp. sputorum a 3563 by a hot phenol-water method and purified by nuclease treatment and by repeated ultracentrifugation. chemical compositions of the purified lps including fatty acid and sugar composition were examined and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) was performed. all lps preparations contained a ... | 1991 | 1916228 |
| monoclonal antibody to a specific antigen from wolinella recta atcc 33238. | four monoclonal antibodies (m-abs) to a specific antigen (150-kda protein antigen) isolated from wolinella recta atcc 33238 by acid extraction were obtained. the four antibodies were all of the igg1 subclass and exhibited equally high specificity for the antigen. a battery of 14 strains or oral bacteria were screened for cross-reactivity with each mab by elisa and western blot analysis. weak cross-reactivity to some strains was observed which differed depending upon the mab. immuno-electromicros ... | 1991 | 1936935 |
| bacteroides ureolyticus (ntu) medium for the selective recovery of bacteroides gracilis. | bacteroides gracilis is a gram-negative anaerobic bacillus which requires formate and fumarate for growth; it has been implicated in periodontal disease and serious infections of the head and neck. in this study, bacteroides ureolyticus (ntu) medium was tested for its ability to allow the growth of b. gracilis and other formate-fumarate requiring gram-negative anaerobes and to enable the recovery of these organisms from clinical specimens. all reference strains grew on ntu medium with the except ... | 1991 | 1942000 |
| analysis of in vitro lymphoproliferative responses and antibody formation following subcutaneous injection of actinobacillus actinomycetemcomitans and wolinella recta in a murine model. | the potential of wolinella recta and actinobacillus actinomycetemcomitans to cause abscesses and induce an immune response was tested in balb/c mice. mice were injected subcutaneously with w. recta, a. actinomycetemcomitans or a mixture of these 2 microorganisms. mice injected with a. actinomycetemcomitans alone, or with both organisms, demonstrated abscesses at the injection site 2 days later, from which pure cultures of a. actinomycetemcomitans were isolated. mice injected with w. recta had sm ... | 1991 | 1945478 |
| dna probe detection of periodontal pathogens in hiv-associated periodontal lesions. | recent studies have shown that an atypical gingivitis and a rapidly progressive periodontal disease may be early-occurring opportunistic infections associated with human immunodeficiency virus (hiv) infection. this study examined the prevalence of selected periodontal pathogens associated with these hiv-related periodontal lesions. subgingival plaque samples were obtained from both hiv-seronegative and hiv-seropositive homosexual men and from presumably uninfected heterosexual men. dna probes we ... | 1991 | 1945481 |
| aspartate and asparagine as electron acceptors for wolinella recta. | since fumarate and nitrate are not usually available in the oral ecosystem, it was investigated whether aspartate and asparagine could be used as alternative electron acceptors by wolinella recta, which is strictly dependent on a respiratory metabolism with formate or h2 as electron donors. both aspartate and asparagine were indeed shown to support growth of w. recta with formate as electron donor. fermentative growth with aspartate alone was not possible. succinate was the major end-product and ... | 1991 | 1945491 |
| dna probe detection of eikenella corrodens, wolinella recta and fusobacterium nucleatum in subgingival plaque. | this cross-sectional study used species-specific dna probes to examine subgingival plaque specimens for the presence of eikenella corrodens, wolinella recta, and fusobacterium nucleatum in adults with untreated periodontitis or gingivitis and in healthy controls. w. recta and f. nucleatum were more prevalent in diseased sites from the periodontitis group when compared with the controls (81% vs 22% and 83% vs 20% respectively). e. corrodens was detected in 62% of the control sites and 81% of the ... | 1991 | 1945492 |
| multivariate analyses of cellular fatty acids in bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp. | the genera bacteroides, wolinella, and campylobacter contain several similar species that require taxonomic revision. fatty acid profiles of whole bacterial cells have proven useful for taxonomy. in this study, cellular fatty acids from bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp. were identified and quantitated by gas chromatography and gas chromatography-mass spectrometry, and the data were subjected to principal component analyses. bacteroides fragilis, the type s ... | 1991 | 1993755 |
| microbiological study of hiv-related periodontitis. | the subgingival microbiota in 14 persons with hiv-periodontitis was examined. subgingival plaque samples were collected with paper points, transported in vmga iii, and plated on anaerobic enriched brucella blood agar and various selective media. hiv-periodontitis sites revealed actinobacillus actinomycetemcomitans, wolinella recta, peptostreptococcus micros, and bacteroides intermedius, each averaging 7% to 16% of the cultivable subgingival flora in positive patients. high levels of spirochetes ... | 1991 | 2002434 |
| development and characterization of porphyromonas gingivalis-specific rat t-cell clones. | porphyromonas gingivalis has been implicated as a major pathogen in periodontitis. to determine the role of t cells in the regulation of this disease, a method was developed for the generation and characterization of rat t-cell clones with antigen specificity to p. gingivalis whole cells. the clones studied so far demonstrated a t-helper (th) phenotype w3/13+, w3/25+, ox8- and ox22-. these t-cell clones proliferated in vitro in response to p. gingivalis, but not to other bacteria (prevotella int ... | 1992 | 1281976 |
| analysis of cell surface antigens of campylobacter rectus. | campylobacter rectus is one of the predominant bacteria in the lesions of human periodontitis. the surface antigens of the bacterium contain several components which may play significant roles in colonization and pathogenesis. a high-molecular-weight protein was selectively isolated from the cell surface of c. rectus by acid extraction and purified by deae sepharose. sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that the extracted protein was 150 kda. the protein was ... | 1992 | 1303324 |
| microbiology of healthy and diseased periodontal sites in poorly controlled insulin dependent diabetics. | a group of poorly-controlled insulin dependent diabetes mellitus (iddm) patients were examined in a cross-sectional design for total microbial levels, microbial incidence, and the percent levels of selected periodontal microorganisms. these organisms were selected on the basis of prior reports that associated them with either periodontal disease or health. one periodontally-healthy and one periodontally-diseased site were examined in each iddm patient. increased levels of the periodontal pathoge ... | 1992 | 1315389 |
| humoral igg antibodies to oral microbiota in a population at risk for root-surface caries. | mutans streptococci have been strongly implicated in the initiation of dental caries on coronal surfaces. their role in development of root-surface caries is less clear. the etiologic agents of both types of dental caries are likely to elicit systemic immune responses. the objective of the present study, therefore, was to study the association of clinical variables of disease with humoral igg antibodies to nine oral micro-organisms in 314 adult subjects, aged 45-65 years, who were at risk for ro ... | 1992 | 1321176 |
| e-test: a new technique for antimicrobial susceptibility testing for periodontal microorganisms. | the purpose of the study was to validate the epsilometer test (e-test) method for antimicrobial susceptibility testing of selected periodontopathic microorganisms using the agar dilution method as a standard. the e-test has been developed to provide a direct quantification of antimicrobial susceptibility of microorganisms. the device consists of a predefined, continuous, and exponential gradient of antibiotic concentrations immobilized along a rectangular plastic test strip. after 48 hours incub ... | 1992 | 1324301 |
| synergistic effect of pyrophosphate and sodium dodecyl sulfate on periodontal pathogens. | our previous studies have shown that pyrophosphate (ppi), the anticalculus component of tartar-control dentifrices, inhibits the growth of organisms associated with coronal and root surface caries. the purposes of this investigation were to: 1) determine if periodontal pathogens are similarly susceptible to the growth-inhibitory properties of ppi; and 2) determine if combinations of pyrophosphate-sodium dodecyl sulfate (ppi-sds) inhibit growth synergistically. porphyromonas gingivalis, actinobac ... | 1992 | 1324304 |
| microbial etiology of periodontal diseases. where are we? where are we going? | many studies indicate a strong association of actinobacillus actinomycetemcomitans with localized juvenile periodontitis. species associated with adult periodontitis include bacteroides forsythus, porphyromonas gingivalis, prevotella intermedia, a. actinomycetemcomitans, and wolinella recta. capnocytophaga species may be important in pubertal gingivitis. an unnamed spirochete related to treponema pallidum has been identified in acute necrotizing ulcerative gingivitis lesions. species isolated fr ... | 1992 | 1325846 |
| cytochrome composition and oxygen-dependent respiration-driven proton translocation in wolinella curva, wolinella recta, bacteroides ureolyticus, and bacteroides gracilis. | the membrane fractions of the microaerobically grown type strains of wolinella curva, wolinella recta, bacteroides ureolyticus, and bacteroides gracilis contained membrane-bound cytochrome b, cytochrome c, and co-binding cytochrome c. soluble cytochrome c and co-binding cytochrome c were also present. although b. gracilis is oxidase negative, it possessed cytochrome c. with h2 or formate as the electron donor, proton efflux from anaerobic cells occurred upon addition of a pulse of oxygen. with f ... | 1992 | 1325865 |
| putative periodontopathogens in "diseased" and "non-diseased" persons exhibiting poor oral hygiene. | the aim of the study was to assess the occurrence of some putative periodonto-pathogens in "test" and "control" sites in "diseased" and "non-diseased" persons, respectively, from an adult rural kenyan population exhibiting poor oral hygiene and widespread loss of attachment (la). 14 persons (less than 35 years) were assigned to a "diseased" category on the basis of at least 4 sites with la greater than or equal to 4 mm; at least 5 mm la and a pocket greater than or equal to 4 mm interproximally ... | 1992 | 1346265 |
| pore-forming ability of major outer membrane proteins from wolinella recta atcc 33238. | three major outer membrane proteins with apparent molecular masses of 43, 45, and 51 kda were purified from wolinella recta atcc 33238, and their pore-forming abilities were determined by the black lipid bilayer method. the non-heat-modifiable 45-kda protein (omp 45) showed no pore-forming activity even at high kcl concentrations. the single-channel conductances in 1 m kcl of the heat-modifiable proteins with apparent molecular masses of 43 kda (omp 43) and 51 kda (omp 51) were 0.49 and 0.60 ns, ... | 1992 | 1370429 |
| specific detection of campylobacter jejuni and campylobacter coli by using polymerase chain reaction. | development of a routine detection assay for campylobacter jejuni and campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction (pcr). an oligonucleotide primer pair from a conserved 5' region of the flaa gene of c. coli vc167 was used to amplify a 450-bp region by pcr. the primer pair specifically detected 4 strains of c. coli and 47 strains of c. jejuni; but it did not detect strains of campylobacter fetus, campylobacter lari, campylobacter upsaliensis, cam ... | 1992 | 1400961 |
| susceptibility of anaerobic microorganisms to hypothiocyanite produced by lactoperoxidase. | the susceptibility of capnocytophaga ochracea, eikenella corrodens, eubacterium yurii, fusobacterium nucleatum, peptostreptococcus micros, prevotella intermedia, selenomonas sputigena, wolinella recta to hypothiocyanite (oscn-) produced by the lactoperoxidase system was tested. results showed a decrease of bacterial survival rate after oscn- exposure, with an intra- and inter-species variability from 0 to 95% for c. ochracea, 34-100% for e. corrodens, 0-83% for e. yurii, 1-15% for f. nucleatum, ... | 1992 | 1481764 |
| associations between microbial species in dental root canal infections. | the existence of commensal or antagonistic relationships between microorganisms in the root canals of teeth with apical periodontitis was investigated. samples were taken from 65 infected human root canals and were analysed according to species, frequency of occurrence and proportion of the total isolated flora. the most frequent species were fusobacterium nucleatum, prevotella intermedia, peptostreptococcus micros, peptostreptococcus anaerobius, eubacterium alactolyticum, eubacterium lentum and ... | 1992 | 1494447 |
| wolinella recta in adult gingivitis and periodontitis. | wolinella recta has been associated with adult periodontitis, but its role in the disease remains uncertain. this report clarifies the distribution of w. recta in periodontally healthy and diseased subjects, and treated patients with recurrent disease. a specific polyclonal rabbit antiserum against w. recta strain 372 was used for indirect immunofluorescence localization of w. recta in dental plaque from untreated and treated patients. supragingival plaque was collected from 15 periodontally hea ... | 1992 | 1531512 |
| production of an extracellular toxin by the oral pathogen campylobacter rectus. | the atcc type strain and six clinical isolates of campylobacter rectus were tested for toxicity against hl-60 cells and human polymorphonuclear neutrophils (pmns). after challenge with bacterial cell suspensions and media supernatants for up to 4 h, eukaryotic cell viability was assayed by trypan blue dye exclusion and lactate dehydrogenase release. cells of the c. rectus type strain were not toxic. however, ethanol and (nh4)2so4 extracts of culture media supernatants killed hl-60 cells in a tim ... | 1992 | 1560755 |
| campylobacter showae sp. nov., isolated from the human oral cavity. | nine campylobacter-like strains were isolated from human gingival crevices and characterized. these strains were gram-negative, straight rods that were motile by means of multiple unipolar flagella. they were asaccharolytic and preferred an anaerobic atmosphere rather than a microaerophilic atmosphere for growth, and their growth was stimulated by formate and fumarate. these strains were biochemically similar to campylobacter curvus and campylobacter rectus, but were clearly distinguishable from ... | 1993 | 7694633 |
| microbial associations in periodontitis sites before and after treatment. | duplicate samples from 110 periodontal sites of 6 mm or more pocket depth in 16 patients were analyzed for the presence of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, capnocytophaga spp., campylobacter rectus, eikenella corrodens and fusobacterium nucleatum. the sites were sampled before and after nonsurgical periodontal treatment. no statistically significant associations were found before treatment between any of the analyzed species. after treatment, ... | 1993 | 8247608 |
| campylobacter rectus in human periodontitis. | campylobacter rectus (formerly wolinella recta) in periodontitis lesions was studied relative to age and sex distribution, relationship to disease-active periodontitis, response to periodontal debridement and in vitro antimicrobial susceptibility. subgingival c. rectus was collected with paper points, transported in vmga iii and plated onto nonselective enriched brucella blood agar and hammond's selective medium for c. rectus, both incubated anaerobically. c. rectus was recovered from 80% of 165 ... | 1993 | 8247610 |
| role of suspected periodontopathogens in microbiological monitoring of periodontitis. | periodontal disease is the clinical result of a complex interaction between the host and plaque bacteria. although a specificity to some degree is found for actinobacillus actinomycetemcomitans in localized juvenile periodontitis (ljp), it has been difficult to obtain evidence for a specific etiological role of the bacteria associated with periodontal disease in adults. what we see is the net result of host-parasite interactions which in an unpredictable moment accumulate and exceed the threshol ... | 1993 | 8260004 |
| dna probe detection of periodontopathogens in advanced periodontitis. | species-specific dna probes were used to determine the presence of actinobacillus actinomycetemcomitans (a.a.), porphyromonas (bacteroides) gingivalis, prevotella intermedia, treponema denticola, eikenella corrodens, fusobacterium nucleatum, and wolinella recta in subgingival plaque from deep pockets/sites of patients with advanced periodontitis. the subjects were 20 patients with severe adult periodontitis, 13 men and 7 women (mean age 45.6 +/- 6.7 yr). for each subject, 9-10 subgingival sites ... | 1993 | 8290878 |
| isolation and partial characterization of the campylobacter rectus cytotoxin. | previously, we reported the antigranulocytic activity of campylobacter rectus media supernatants containing lipopolysaccharide (lps) and a 104 kda protein. here, we monitored the release of protein and lps through the growth cycle of c. rectus atcc 33238 and identified the 104 kda protein as the cytotoxin. lps in media supernatants was quantitated by a kdo assay; the 104 kda protein was detected on immunoblots with specific antibody (a104) and quantitated by amino acid analysis of membrane immob ... | 1993 | 8321122 |
| recent taxonomic changes for anaerobic gram-positive and selected gram-negative organisms. | the strains formerly classified as streptococcus anginosus or "streptococcus milleri" have now been recognized as three distinct species, streptococcus anginosus, streptococcus constellatus, and streptococcus intermedius. streptococcus morbillorum has been transferred into the genus gemella. four new species within the genus peptostreptococcus were recently named. a recent addition to the genus clostridium is clostridium argentinense, which includes clostridium botulinum type g. two new species ... | 1993 | 8324113 |
| oral and dental infections with anaerobic bacteria: clinical features, predominant pathogens, and treatment. | microbial populations colonizing the teeth are a major source of pathogens responsible for oral and dental infections, including periodontal diseases, gingivitis, pericoronitis, endodontitis, peri-implantitis, and postextraction infections. each entity has distinct clinical and microbial features. bacterial species associated with oral infections include actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, bacteroides forsythus, campylobacter rectus, eubacterium ... | 1993 | 8324136 |
| suppression of proliferation of a human b-cell leukaemic cell line derived from acute lymphoblastic leukaemia by soluble factor(s) from campylobacter rectus. | soluble sonic extracts of several strains were examined for their ability to alter proliferation of a cell line derived from acute lymphoblastic leukaemia (ball-1). extracts of all strains tested caused dose-dependent suppression of proliferation when assessed by dna (tritiated thymidine incorporation), rna (tritiated uridine incorporation) and protein (tritiated leucine incorporation) synthesis. there was no effect on the viability of ball-1 as measured by either trypan-blue exclusion or extrac ... | 1993 | 8343067 |
| helicobacter acinonyx sp. nov., isolated from cheetahs with gastritis. | four strains of a novel helicobacter species were isolated from the stomachs of cheetahs (acinonyx jubilatus) with gastritis. these isolates were phenotypically similar to helicobacter pylori. the isolates were gram-negative, spiral bacteria which grew under microaerophilic conditions at 37 degrees c, but not at 25 or 42 degrees c, and produced urease, catalase, oxidase, alkaline phosphatase, and gamma-glutamyl transpeptidase. the isolates did not ferment glucose, mannitol, inositol, sorbitol, r ... | 1993 | 8379970 |
| microbiological and clinical monitoring of non-localized juvenile periodontitis in young adults: a report of 11 cases. | it has been shown that patients with localized juvenile periodontitis (ljp) often harbor actinobacillus actinomycetemcomitans in the subgingival area. however, little is known of the oral microflora in non-ljp juvenile periodontitis patients with less extensive disease. the purpose of this study was to describe the microflora and clinical parameters of young adults with minor to moderate periodontitis during treatment for a period of 1 year. eleven patients 15 to 16 years of age were studied. al ... | 1993 | 8381179 |
| effect of therapy on periodontal infections. | periodontal disease progression requires the simultaneous presence of high numbers of pathogens, low numbers of compatible or beneficial species, a conductive local environment, and a susceptible host. effective therapy acts by altering one or more of these factors. data from an ongoing study were used to examine the biological basis of treatment success or failure. seventeen subjects showing disease progression were treated by widman flap surgery at deep sites, scaling at shallow sites, and 1 o ... | 1993 | 8410615 |
| microbial composition and pattern of antibiotic resistance in subgingival microbial samples from patients with refractory periodontitis. | a series of 993 subgingival microbial samples sent to a diagnostic microbiology laboratory included 196 samples that could be identified as compatible with a clinical diagnosis of refractory or recurrent periodontitis. in descending order of prevalence the associated microbiota included bacteroides forsythus (84%), spirochetes (83%), motile rods (76%), fusobacterium species (68%), porphyromonas gingivalis (63%), campylobacter rectus (47%), capnocytophaga species (38%), prevotella intermedia (23% ... | 1993 | 8463936 |
| an analysis of the subgingival microflora in randomly selected subjects. | the aim of this study was to describe the presence of some microbial species in the subgingival microflora of a randomly selected subject sample. a further objective was to analyze the association between some microbiological characteristics and the periodontal conditions of the subjects. a total of 171 individuals aged 30-65 years were included. a pooled subgingival plaque sample was obtained from the mesial aspect of the 6 ramfjord teeth by the use of the paper-point method. the samples were p ... | 1993 | 8510980 |
| aspartame as a source of essential phenylalanine for the growth of oral anaerobes. | phenylalanine and aspartic acid requirements were determined for 13 species of oral bacteria using the chemically defined medium omiz-w1. none of actinobacillus actinomycetemcomitans, bacteroides forsythus, eikenella corrodens, selenomonas sputigena, treponema pectinovorum, t. socranskii, or wolinella recta required either of these amino acid constituents of aspartame (l-aspartyl-l-phenylalanine methylester). phenylalanine was essential for the growth of capnocytophaga gingivalis, eubacterium ti ... | 1993 | 8514113 |
| antigenic properties of campylobacter rectus (wolinella recta) major s-layer proteins. | the antigenic properties of the surface layer (s-layer) proteins of various campylobacter rectus strains including 24 clinical isolates and the type strain atcc 33238 were examined. s-layer proteins were extracted from whole cells by acid treatment according to the method of mccoy et al. (infect. immun. 11, 517-525, 1975). the acid extracts from 23 of the isolates and atcc 33238 contained two major proteins with molecular masses of 130 kda and 150 kda, both of which were identified as subunits o ... | 1993 | 8514115 |
| effect of initial therapy on dynamics of immunogloblin g levels to some periodontopathic bacteria in serum and gingival crevicular fluid. | using enzyme-linked immunosorbent assay, the igg antibody levels to specific gram-negative periodontopathic bacteria in serum of 21 adult periodontitis patients and the gingival crevicular fluid (gcf) from 42 sites with destructive lesions and 21 healthy sites were evaluated before and after initial preparation. porphyromonas gingivalis, bacteroides forsythus, prevotella intermedia, prevotella nigrescens, campylobacter rectus, treponema denticola and actinobacillus actinomycetemcomitans in subgi ... | 1994 | 8689734 |
| bacteriological diagnosis of periodontal disease. | dental plaque bacteria cause virtually all the forms of inflammatory periodontal disease. periodontitis is caused by the specific periodontopathic bacteria, which induce destruction of connective tissue attachment and adjacent alveolar bone. examinations to identify the infections by porphyromonas gingivalis, actinobacillus actinomycetemcomitans, prevotella intermedia, campylobacter rectus, bacteroides forsythus, fusobacterium nucleatum, eikenella corrodens and treponema denticola have recently ... | 1994 | 8620588 |