Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| porcine intestinal adenomatosis: a possible relationship with necrotic enteritis, regional ileitis and proliferative haemorrhagic enteropathy. | necrotic enteritis and regional ileitis are recognised causes of sporadic death in pigs. in both conditions, the intestinal mucosa harbours the bacterium, campylobacter sputorum subsp mucosalis, which was first associated with procine intestinal adenomatosis. | 1975 | 1080616 |
| isolation of campylobacter sputorum subsp mucosalis from oral cavity of pigs. | 1975 | 1189242 | |
| studies on campylobacter sputorum subspecies mucosalis. | catalase-negative vibrios can be isolated in large numbers from the affected intestinal mucosa of pigs suffering from a range of porcine enteropathies in which the mucosa has an adenomatous component. these vibrios cannot be distinguished from strains of campylobacter sputorum subsp. mucosalis. the resemblance between these bacteria strengthens the case already made on morphological evidence that these enteropathies have a common origin. catalase-negative vibrios have also been isolated from the ... | 1976 | 933148 |
| the surface antigens of campylobacter sputorum subspecies mucosalis. | campylobacter sputorum subsp mucosalis is the vibrio associated with a group of porcine enteropathies in which it is believed the primary lesion is that of adenomatosis. the surface antigens of mucosalis were investigated and individual factor sera were produced; these were used to examine a range of field strains from adenomatosis, necrotic enteritis, regional ileitis and proliferative haemorrhagic enteropathy. neither these not sequential isolants from the same farm showed any distinctive anti ... | 1977 | 605305 |
| proliferative haemorrhagic enteropathy. | this study indicates that viable campylobacter sputorum subsp mucosalis are not present or are present in small numbers in the mucosa of pigs dying of proliferative haemorrhagic enteropathy. the changes present in the mucosa are similar to those seen in pigs recovering from adenomatosis and the evidence obtained indicates that the intracellular organisms observed in this condition are indeed mucosalis. the presence of large amounts of iga in the altered tissue of both proliferative haemorrhagic ... | 1979 | 315603 |
| the experimental infection of neonatal pigs with campylobacter sputorum subspecies mucosalis. | pigs dosed orally with campylobacter sputorum subsp mucosalis in the neonatal period became infected and mucosalis was recovered from the intestinal mucosa for up to 40 days after infection. rapid spread to undosed litter mates occurred. gross adenomatous change did not develop. | 1980 | 7414059 |
| the experimental infection of pigs with campylobacter sputorum subspecies mucosalis. weaned pigs, with special reference to pharmacologically mediated hypomotility. | the weaned pig proved resistant to infection with campylobacter sputorum subsp mucosalis after oral dosing. pretreatment with benzetimide to reduce gastrointestinal peristalsis allowed mucosalis infection to be established. | 1980 | 7414060 |
| agglutinins to bacteria in crohn's disease. | sera from patients with crohn's disease were tested for antibodies against organisms which are thought to cause inflammatory bowel disease in animals, or have been implicated in human crohn's disease. control sera were collected from healthy individuals and patients with ulcerative colitis. sera from crohn's disease and controls failed to agglutinate clostridium colinum or campylobacter sputorum subsp. mucosalis and two strains of mycobacterium paratuberculosis (m26 and m27). most of the sera ag ... | 1980 | 7429299 |
| experimentally induced porcine proliferative enteritis in specific-pathogen-free pigs. | thirty-three, 10-week-old, specific-pathogen-free pigs were randomly allotted to 3 treatment groups: group 1--intragastrically given homogenized intestinal mucosa (crude inoculum) from pigs with naturally occurring proliferative enteritis; group 2--given cultures of campylobacter sputorum subsp mucosalis; and group 3--controls. one pig from each group was killed 4, 7, 10, 14, 18, 21, 24, 28, 31, 36, and 38 days after inoculation. the earliest intestinal lesion observed in groups 1 and 2 was leuk ... | 1982 | 6216836 |
| naturally occurring porcine proliferative enteritis: pathologic and bacteriologic findings. | the lesions of porcine proliferative enteritis were studied by light and electron microscopic techniques in feeder pigs, fattening hogs, bred gilts, sows, and boars. the characteristic microscopic feature common to all age groups was proliferation of immature crypt epithelial cells, primarily in the ileum and the distal part of the jejunum. similar changes were also observed in the midjejunum, cecum, and colon of a new swine. the earliest detectable microscopic lesion was focal proliferation of ... | 1982 | 7149407 |
| porcine proliferative enteritis: experimentally induced disease in cesarean-derived colostrum-deprived pigs. | the hypotheses that porcine proliferative enteritis is an infectious disease and that campylobacter sputorum subsp mucosalis (csm) is involved in the development of this disease were experimentally tested. three experiments were conducted with 10-week-old, cesarean-derived colostrum-deprived pigs. of 22 pigs given homogenized mucosal scrapings (crude inocula) intragastrically, 15 had gross and/or microscopic lesions of proliferative enteritis. of 10 pigs inoculated with cultures of both csm and ... | 1982 | 7149408 |
| immunofluorescent demonstration of campylobacter hyointestinalis and campylobacter sputorum subsp mucosalis in swine intestines with lesions of proliferative enteritis. | an indirect fluorescent antibody technique was developed to identify campylobacter spp in lesions of swine proliferative enteritis (spe). rabbit antisera to c hyointestinalis and c sputorum subsp mucosalis were produced. bacterial smears stained by fluorescent antibody test with homologous antisera differentiated c hyointestinalis from subsp mucosalis. ileal frozen sections from 29 pigs with histologic lesions of spe had specific fluorescent staining of c hyointestinalis in all 29 and subsp muco ... | 1984 | 6375483 |
| isoprenoid quinone content and cellular fatty acid composition of campylobacter species. | a total of 36 strains of campylobacter species were examined for isoprenoid quinones and cellular fatty acids. the isoprenoid quinone content was determined by reverse-phase high-pressure liquid chromatography, and the fatty acids were determined by capillary gas-liquid chromatography. all campylobacter species contained menaquinone-6 (2-methyl-3-farnesyl-farnesyl-1,4-naphthoquinone) and a methyl-substituted menaquinone-6 (2,[5 or 8]-dimethyl-3-farnesyl-farnesyl-1,4-napthoquinone) as the major i ... | 1984 | 6470096 |
| isolation of campylobacter sputorum subsp. mucosalis in norway. | 1984 | 6534167 | |
| in vitro activities of 47 antimicrobial agents against three campylobacter spp. from pigs. | the in vitro activities of 47 antimicrobial agents against 30 isolates of campylobacter species from pigs were determined by the agar dilution technique. the isolates were obtained from pigs with proliferative enteritis and included 10 strains each of campylobacter coli, campylobacter sputorum subsp. mucosalis, and "campylobacter hyointestinalis gebhart et al." (this name is not on the approved lists). carbadox, furazolidone, nitrofurantoin, gentamicin, and dimetridazole were the most active dru ... | 1985 | 3985597 |
| campylobacter sputorum subsp mucosalis and campylobacter hyointestinalis infections in the intestine of gnotobiotic pigs. | at 4 days of age, 7 gnotobiotic pigs were orally inoculated with broth cultures of both campylobacter sputorum subsp mucosalis (csm) and campylobacter hyointestinalis (ch). one pig was killed and evaluated each week for 7 weeks. forty-eight hours after inoculation, ch and csm were recovered from the feces of the pigs; thereafter, only ch was recovered. organisms morphologically typical of campylobacter sp were observed on the mucosal surface and on the crypt epithelial cells of the ileum, cecum, ... | 1985 | 4062022 |
| dna homology studies of the catalase-negative campylobacters and "campylobacter fecalis," an emended description of campylobacter sputorum, and proposal of the neotype strain of campylobacter sputorum. | twenty-three strains of catalase-negative campylobacters and five strains of "campylobacter fecalis," which is catalase-positive, were examined by dna hybridization experiments. these organisms formed four distinct dna homology groups corresponding to campylobacter sputorum, campylobacter mucosalis, campylobacter concisus, and a currently unnamed group referred to as the "catalase-negative or weak" (cnw) strains. the strains were further characterized to determine which phenotypic characteristic ... | 1985 | 4084862 |
| possible relationship of proliferative enteritis in pigs and hamsters. | three- to six-week-old hamsters were orally inoculated with broths containing one of the following cultures: campylobacter mucosalis; c. hyointestinalis; c. coli; c. jejuni, all of porcine proliferative enteritis origin, or else c. jejuni of hamster origin. hamsters given the last of those organisms were shown to have colonisation of their intestines by c. jejuni and 36 of 40 developed an acute enteritis. mild hyperplasia of enterocytes in ileal crypts was evident in one hamster 2 days after it ... | 1987 | 3326247 |
| the microaerophilic respiration of campylobacter mucosalis. | a model is proposed for the respiratory adaptation to falling oxygen concentration during growth of the microaerophilic bacterium campylobacter mucosalis. during the early stages of growth, the oxidation of formate is a two-stage branched process involving the production of h2o2 followed by its peroxidatic removal. in later stages of growth, at lower oxygen concentrations, the predominant electron flow is linear to a membrane-bound cytochrome-c oxidase which reduces o2 directly to h2o. several c ... | 1988 | 2831975 |
| serological diagnosis of the porcine proliferative enteropathies: implications for aetiology and epidemiology. | campylobacter mucosalis and c hyointestinalis have been associated with the proliferative enteropathies of pigs. an examination of the antibody response to these organisms and to the intracellular campylobacter-like organism was undertaken. antibody to the campylobacter-like organism was predominantly igm, short lived, and could be detected by an immunofluorescence test using bacteria released from lesions as antigen. the majority (75 per cent) of pigs with proliferative enteropathy at necropsy ... | 1988 | 3046108 |
| infection of established cell lines with campylobacter mucosalis. | campylobacter mucosalis (cm) and other campylobacter spp. mainly from cases of the porcine proliferative enteropathies were used to infect cell line cultures. cm attached to and persisted intracellularly in pk (15), mdbk, bhk, hela, int-407 and other cells; in pk (15) this infection resulted in a marked cytopathic effect and total destruction of the cell culture in 10 days. mdbk, int-407, bhk and hela cultures were more resistant to the effects of infection. cm did not adhere to vero, llcmk2 or ... | 1988 | 3140471 |
| the first isolations of campylobacter mucosalis from pigs in south africa. | the first isolations of campylobacter mucosalis in south africa are described. isolations were made from a 6-week-old weaner pig with necrotic enteritis and from 2 gingival swabs of suckling piglets from herds with histories of porcine intestinal adenomatosis. the isolates were serologically identified as being serotype a strains. | 1988 | 3194117 |
| new selective media for the isolation of campylobacter mucosalis and campylobacter hyointestinalis. | 1988 | 3199619 | |
| an extended serotyping scheme for campylobacter mucosalis isolated from proliferative enteritis in swine. | 1988 | 3199628 | |
| electrophoretic protein patterns in campylobacter species with special reference to campylobacter mucosalis and campylobacter hyointestinalis. | 1988 | 3210482 | |
| antigenic analysis of campylobacter species and an intracellular campylobacter-like organism associated with porcine proliferative enteropathies. | whole-cell and outer membrane preparations of campylobacter mucosalis, c. hyointestinalis, c. jejuni, and c. coli isolated from porcine intestines were compared with preparations of intracellular campylobacter-like organisms extracted directly from the lesions of pigs with proliferative enteropathy. by gradient polyacrylamide gel electrophoresis, outer membrane and total protein profiles of c. mucosalis, c. hyointestinalis, c. jejuni, and c. coli were significantly different from each other and ... | 1989 | 2917794 |
| immuno-histochemical and -cytochemical evidence suggesting the presence of campylobacter jejuni and campylobacter coli in cases of porcine intestinal adenomatosis. | antisera against a number of campylobacter species were used in immuno-histochemical and -cytochemical studies on cases of porcine intestinal adenomatosis. avidin-biotin-complex (abc) and streptavidin immunoperoxidase methods were used on formalin-fixed, paraffin-embedded and frozen sections. protein a gold method was used on formaldehyde fixed and frozen sections for immuno-cytochemistry. the antisera used were raised in rabbits by subcutaneous or intravenous injection of living or formalin tre ... | 1990 | 2099622 |
| effect of enteroviruses on adherence to and invasion of hep-2 cells by campylobacter isolates. | coinfection of hep-2 epithelial cells with coxsackievirus b3, echovirus 7, poliovirus (lsc type 1), porcine enterovirus, and campylobacter isolates was performed to determine if a synergistic effect could be obtained. the invasiveness of campylobacter jejuni atcc 33560 was significantly increased for hep-2 cells preinfected with echovirus 7, coxsackievirus b3, and uv-inactivated (noninfectious) coxsackievirus b3 particles. additionally, the invasiveness of c. jejuni m96, a clinical isolate, was ... | 1990 | 2156779 |
| southern blot analysis of strain variation in campylobacter mucosalis. | a panel of three dna probes were derived at random from a genomic dna library of campylobacter mucosalis strain e8384-4. each probe hybridized specifically to c. mucosalis dna from bacteria fixed to nylon membranes. the probes did not hybridize to dna from other campylobacter species or to other bacteria even at 100-fold higher amounts. each probe hybridized to all of 24 isolates of c. mucosalis which had been collected over time from different geographic locations. southern blot analysis of sel ... | 1991 | 1673809 |
| revision of campylobacter, helicobacter, and wolinella taxonomy: emendation of generic descriptions and proposal of arcobacter gen. nov. | hybridization experiments were carried out between dnas from more than 70 strains of campylobacter spp. and related taxa and either 3h-labeled 23s rrnas from reference strains belonging to campylobacter fetus, campylobacter concisus, campylobacter sputorum, campylobacter coli, and campylobacter nitrofigilis, an unnamed campylobacter sp. strain, and a wolinella succinogenes strain or 3h- or 14c-labeled 23s rrnas from 13 gram-negative reference strains. an immunotyping analysis of 130 antigens ver ... | 1991 | 1704793 |
| use of embryonating eggs for isolation of campylobacter species from intestines of swine with proliferative enteritis. | intestinal tissues from swine affected with proliferative enteritis were ground, filtered through a 0.65-micron pore membrane filter, diluted, and injected into 7-day-old embryonated hens' eggs via the yolk sac. at 2, 4, and 7 days later, yolk sac swab specimens taken from live embryos were cultured for campylobacter species. campylobacter hyointestinalis was recovered from eggs injected with tissues of swine with acute hemorrhagic proliferative enteritis at dilutions up to 10(-4). campylobacter ... | 1991 | 1883083 |
| clinical isolates of campylobacter mucosalis. | 1993 | 8254000 | |
| two cases of campylobacter mucosalis enteritis in children. | two cases of campylobacter mucosalis enteritis in children are reported. the patients recovered without antimicrobial therapy. strains were isolated only by the feces filtration technique. in one child, bactericidal antibodies to the homologous strain were detected in a convalescent-phase serum sample. c. mucosalis should be considered a primary intestinal pathogen. | 1993 | 8458973 |
| confirmation of human campylobacter concisus isolates misidentified as campylobacter mucosalis and suggestions for improved differentiation between the two species. | a strain from human diarrhea originally identified as campylobacter mucosalis (nctc 12408) was examined by using 64 phenotypic characters. the similarity of this strain to 297 isolates of campylobacter, helicobacter, arcobacter, and related taxa was then determined with a computer-supported data analysis program, mvsp. nctc 12408 showed closest similarity to 20 type, reference, and field isolates of campylobacter concisus. these strains were clearly separated from those of c. mucosalis in the nu ... | 1994 | 7529255 |
| additional data on clinical isolates of campylobacter mucosalis. | 1994 | 7814574 | |
| campylobacter hyoilei sp. nov., associated with porcine proliferative enteritis. | campylobacter hyoilei sp. nov. is the name proposed for an organism formerly described as strain rmit 32at (t = type strain) and a group of similar bacteria isolated from intestinal lesions of pigs with proliferative enteritis. the phenotypic characteristics of these organisms indicated that they are closely related to each other and are not strains of other campylobacter spp. commonly isolated from pigs. the results of probing of clai-, ecorv-, or bglii-cleaved genomic dnas from c. hyoilei stra ... | 1995 | 7857809 |
| detection of small numbers of campylobacter jejuni and campylobacter coli cells in environmental water, sewage, and food samples by a seminested pcr assay. | a rapid and sensitive assay was developed for detection of small numbers of campylobacter jejuni and campylobacter coli cells in environmental water, sewage, and food samples. water and sewage samples were filtered, and the filters were enriched overnight in a nonselective medium. the enrichment cultures were prepared for pcr by a rapid and simple procedure consisting of centrifugation, proteinase k treatment, and boiling. a seminested pcr based on specific amplification of the intergenic sequen ... | 1999 | 10103261 |
| specific pcr identification and differentiation of the thermophilic campylobacters, campylobacter jejuni, c. coli, c. lari, and c. upsaliensis. | a sensitive pcr assay that detects the thermophilic campylobacters c. jejuni, c. coli, c. lari, and c. upsaliensis is reported. furthermore, by digestion of the pcr products with two restriction enzymes, species differentiation was demonstrated. thus, the present method has the potential to be used for both detection and identification of thermophilic campylobacter species. | 1999 | 10488210 |
| rapid identification of campylobacter, arcobacter, and helicobacter isolates by pcr-restriction fragment length polymorphism analysis of the 16s rrna gene. | a rapid two-step identification scheme based on pcr-restriction fragment length polymorphism (pcr-rflp) analysis of the 16s rrna gene was developed in order to differentiate isolates belonging to the campylobacter, arcobacter, and helicobacter genera. for 158 isolates (26 reference cultures and 132 clinical isolates), specific rflp patterns were obtained and species were successfully identified by this assay. | 1999 | 10565952 |
| prevalence of campylobacter, arcobacter, helicobacter, and sutterella spp. in human fecal samples as estimated by a reevaluation of isolation methods for campylobacters. | the aims of this study were to investigate the prevalence of campylobacteria including campylobacter jejuni subsp. jejuni (c. jejuni) and campylobacter coli in human clinical samples and in samples from healthy individuals and to reevaluate the efficacies of conventional selective methods for isolation of campylobacter spp. two charcoal-based selective media, modified charcoal cefoperazone deoxycholate agar (mccda) and cefoperazone-amphotericin-teicoplanin (cat) agar, were compared with skirrow' ... | 2000 | 10618103 |
| identification of campylobacter jejuni, c. coli, c. lari, c. upsaliensis, arcobacter butzleri, and a. butzleri-like species based on the glya gene. | currently, the detection and identification of campylobacter and arcobacter species remains arduous, largely due to cross-species phenotypic similarities and a relatively narrow spectrum of biochemical reactivity. we have developed a pcr-hybridization strategy, wherein degenerate primers are used to amplify glya fragments from samples, which are then subjected to species-specific oligodeoxyribonucleotide probe hybridizations, to identify and distinguish between campylobacter jejuni, c. coli, c. ... | 2000 | 10747131 |
| campylobacter lanienae sp. nov., a new species isolated from workers in an abattoir. | campylobacter-like organisms were isolated from the faeces of healthy individuals during a hygiene survey of abattoir workers. the strains, which exhibited characteristics of campylobacter, being non-glucose-fermenting, oxidase- and catalase-positive, gram-negative, motile rods, were identified to the genus level by a pcr assay. nucleotide sequence analysis of the 16s rrna gene, dna homology experiments and determination of g + c content demonstrated that they constituted a previously undescribe ... | 2000 | 10758898 |
| colonic infection by bilophila wadsworthia in pigs. | bilophila wadsworthia is a common inhabitant of the human colon and has been associated with appendicitis and other local sites of inflammation in humans. challenge-exposure or prevalence studies in laboratory and other animals have not been reported. b. wadsworthia is closely related phylogenetically to desulfovibrio sp. and lawsonia intracellularis, which are considered colon pathogens. we developed a pcr specific for b. wadsworthia dna. samples of bacterial dna extracted from the feces of pig ... | 2001 | 11283090 |
| molecular identification of campylobacter concisus. | a 1.6-kb dna fragment isolated from a campylobacter concisus genomic library gave c. concisus-specific restriction fragment length patterns when it was used as a probe in hybridization studies. all of the strains tested, including type strains and clinical isolates, contained a 0.5-kb hindiii fragment that hybridized to the probe. dna sequencing of the 1.6-kb fragment identified three open reading frames (orfs). one of the orfs encodes the carboxy terminus of gyrb, and the translational products ... | 2001 | 11574591 |
| differentiation of campylobacter species by aflp fingerprinting. | the fluorescent amplified fragment length polymorphism (aflp) fingerprinting method was tested for its ability to identify and subtype the most important campylobacter species found in veterinary infections. sixty-nine reference strains and 19 clinical isolates of campylobacter jejuni subsp. jejuni, campylobacter jejuni subsp. doylei, campylobacter upsaliensis, campylobacter coli, campylobacter lari, campylobacter fetus subsp. fetus, c. fetus subsp. venerealis, campylobacter hyointestinalis subs ... | 2001 | 11577152 |
| evaluation of a novel heminested pcr assay based on the phosphoglucosamine mutase gene for detection of helicobacter pylori in saliva and dental plaque. | a novel heminested pcr protocol was developed for the specific detection of helicobacter pylori at low copy numbers. a set of primers specific for the phosphoglucosamine mutase gene (glmm) of h. pylori produced a 765-bp fragment that was used as template for the heminested primer pair delineating a 496-bp fragment. by using agarose gel electrophoresis for detection of the heminested pcr-amplified products, amplification of h. pylori genomic dna was achieved at concentrations as low as 0.1 pg, eq ... | 2002 | 11773117 |
| growth of campylobacter jejuni supported by respiration of fumarate, nitrate, nitrite, trimethylamine-n-oxide, or dimethyl sulfoxide requires oxygen. | the human gastrointestinal pathogen campylobacter jejuni is a microaerophilic bacterium with a respiratory metabolism. the genome sequence of c. jejuni strain 11168 reveals the presence of genes that encode terminal reductases that are predicted to allow the use of a wide range of alternative electron acceptors to oxygen, including fumarate, nitrate, nitrite, and n- or s-oxides. all of these reductase activities were present in cells of strain 11168, and the molybdoenzyme encoded by cj0264c was ... | 2002 | 12107136 |
| prevalence of campylobacter jejuni, campylobacter lari, and campylobacter coli in different ecological guilds and taxa of migrating birds. | a total of 1,794 migrating birds trapped at a coastal site in southern sweden were sampled for detection of campylobacter spp. all isolates phenotypically identified as campylobacter jejuni and a subset of those identified as non-c. jejuni were identified to the species level by pcr-based techniques. c. jejuni was found in 5.0% of the birds, campylobacter lari was found in 5.6%, and campylobacter coli was found in 0.9%. an additional 10.7% of the tested birds were infected with hippurate hydroly ... | 2002 | 12450810 |
| evaluation of 11 pcr assays for species-level identification of campylobacter jejuni and campylobacter coli. | we examined the sensitivity and specificity of 11 pcr assays described for the species identification of campylobacter jejuni and campylobacter coli by using 111 type, reference, and field strains of c. jejuni, c. coli, and campylobacter lari. for six assays, an additional 21 type strains representing related campylobacter, arcobacter, and helicobacter species were also included. pcr tests were initially established in the laboratory by optimizing conditions with respect to five type and referen ... | 2003 | 12517869 |
| a real-time pcr assay for the detection of campylobacter jejuni in foods after enrichment culture. | a real-time pcr assay was developed for the quantitative detection of campylobacter jejuni in foods after enrichment culture. the specificity of the assay for c. jejuni was demonstrated with a diverse range of campylobacter species, related organisms, and unrelated genera. the assay had a linear range of quantification over six orders of magnitude, and the limit of detection was approximately 12 genome equivalents. the assay was used to detect c. jejuni in both naturally and artificially contami ... | 2003 | 12620820 |
| use of pcr for direct detection of campylobacter species in bovine feces. | this study reports on the use of pcr to directly detect and distinguish campylobacter species in bovine feces without enrichment. inhibitors present in feces are a major obstacle to using pcr to detect microorganisms. the qiaamp dna stool minikit was found to be an efficacious extraction method, as determined by the positive amplification of internal control dna added to bovine feces before extraction. with nested or seminested multiplex pcr, campylobacter coli, c. fetus, c. hyointestinalis, and ... | 2003 | 12788747 |
| web-based phylogenetic assignment tool for analysis of terminal restriction fragment length polymorphism profiles of microbial communities. | culture-independent dna fingerprints are commonly used to assess the diversity of a microbial community. however, relating species composition to community profiles produced by community fingerprint methods is not straightforward. terminal restriction fragment length polymorphism (t-rflp) is a community fingerprint method in which phylogenetic assignments may be inferred from the terminal restriction fragment (t-rf) sizes through the use of web-based resources that predict t-rf sizes for known b ... | 2003 | 14602639 |
| anaerobic bacteria cultured from the tongue dorsum of subjects with oral malodor. | the bacteria on the dorsum of the tongue are the most frequent cause of oral malodor; however, the bacterial flora of the tongue has not been well defined. although recent studies have used dna probes to detect the presence of certain periodontal pathogens, cultural studies have been limited because of the complexity of the flora of the tongue dorsum. the purpose of this study was to grow and to identify maximum numbers of capnophylic gram-negative bacilli and anaerobic micro-organisms by cultur ... | 2003 | 16887710 |
| multiplex pcr assay for differentiation of helicobacter felis, h. bizzozeronii, and h. salomonis. | helicobacter felis, helicobacter bizzozeronii, and helicobacter salomonis are frequently found in the gastric mucous membrane of dogs and cats. these large spiral organisms are phylogenetically highly related to each other. their fastidious nature makes it difficult to cultivate them in vitro, hampering traditional identification methods. we describe here a multiplex pcr test based on the trna intergenic spacers and on the urease gene, combined with capillary electrophoresis, that allows discrim ... | 2004 | 15004062 |
| direct quantification of campylobacter jejuni and campylobacter lanienae in feces of cattle by real-time quantitative pcr. | campylobacter species are fastidious to culture, and the ability to directly quantify biomass in microbiologically complex substrates using real-time quantitative (rtq) pcr may enhance our understanding of their biology and facilitate the development of efficacious mitigation strategies. this study reports the use of nested rtq-pcr to directly quantify campylobacter jejuni and campylobacter lanienae in cattle feces. for c. jejuni, the single-copy mapa gene was selected. for c. lanienae, the thre ... | 2004 | 15066825 |
| detection of campylobacter spp. in chicken fecal samples by real-time pcr. | a real-time pcr assay for detecting thermophilic campylobacter spp. directly in chicken feces has been developed. dna was isolated from fecal material by using magnetic beads followed by pcr with a prealiquoted pcr mixture, which had been stored at -18 degrees c. campylobacter could be detected in less than 4 h, with a detection limit of 100 to 150 cfu/ml, in a fecal suspension. a bacterial internal control was added before dna extraction to control both dna isolation and the presence of pcr inh ... | 2004 | 15528705 |
| differentiation of campylobacter coli, campylobacter jejuni, campylobacter lari, and campylobacter upsaliensis by a multiplex pcr developed from the nucleotide sequence of the lipid a gene lpxa. | we describe a multiplex pcr assay to identify and discriminate between isolates of campylobacter coli, campylobacter jejuni, campylobacter lari, and campylobacter upsaliensis. the c. jejuni isolate f38011 lpxa gene, encoding a udp-n-acetylglucosamine acyltransferase, was identified by sequence analysis of an expression plasmid that restored wild-type lipopolysaccharide levels in escherichia coli strain sm105 [lpxa(ts)]. with oligonucleotide primers developed to the c. jejuni lpxa gene, nearly fu ... | 2004 | 15583280 |
| comparison of 2 serologic tests for the diagnosis of porcine proliferative enteropathy. | the purpose of this study was to compare results from 2 serological assays at the individual- and herd-level for porcine proliferative enteropathy diagnosis. cohen's kappa coefficient (k) was used to measure agreement. the tests tend to show better agreement when used at the herd level. | 2005 | 16018563 |
| identification of candidate periodontal pathogens and beneficial species by quantitative 16s clonal analysis. | most studies of the bacterial etiology of periodontitis have used either culture-based or targeted dna approaches, and so it is likely that pathogens remain undiscovered. the purpose of this study was to use culture-independent, quantitative analysis of biofilms associated with chronic periodontitis and periodontal health to identify pathogens and beneficial species. samples from subjects with periodontitis and controls were analyzed using ribosomal 16s cloning and sequencing. several genera, ma ... | 2005 | 16081935 |
| human inflammatory bowel disease does not associate with lawsonia intracellularis infection. | there is increasing evidence that bacterial infection of the intestinal mucosa may contribute to the pathogenesis of inflammatory bowel diseases (ibd). in pigs, an obligate intracellular bacterium, lawsonia intracellularis (li), was shown to cause proliferative enteropathy (pe) of which some forms display histological and clinical similarities to human ibd. since li-similar desulfovibrio spp. may infect human cells, we hypothesized that li might be associated with the development of human ibd. | 2006 | 16984651 |
| development of a real-time fluorescence resonance energy transfer pcr to detect arcobacter species. | a real-time pcr targeting the gyrase a subunit gene outside the quinolone resistance-determining region has been developed to detect arcobacter species. the species identification was done by probe hybridization and melting curve analysis, using fluorescence resonance energy transfer technology. discrimination between arcobacter species was straightforward, as the corresponding melting points showed significant differences with the characteristic melting temperatures of 63.5 degrees c, 58.4 degr ... | 2007 | 17652482 |
| characterization of two putative cytochrome c peroxidases of campylobacter jejuni involved in promoting commensal colonization of poultry. | campylobacter jejuni is a leading cause of bacterial gastroenteritis in humans throughout the world, but infection of animals, especially poultry, results in a commensal colonization of the intestines. we previously found that a mutant lacking doca, which encodes a putative cytochrome c peroxidase (ccp), demonstrates up to a 10(5)-fold reduction in colonization of the chick cecum compared to wild-type c. jejuni strain 81-176. predictions from genomic sequences identified cjj0382 as a second locu ... | 2008 | 18086814 |
| characterization of two putative cytochrome c peroxidases of campylobacter jejuni involved in promoting commensal colonization of poultry. | campylobacter jejuni is a leading cause of bacterial gastroenteritis in humans throughout the world, but infection of animals, especially poultry, results in a commensal colonization of the intestines. we previously found that a mutant lacking doca, which encodes a putative cytochrome c peroxidase (ccp), demonstrates up to a 10(5)-fold reduction in colonization of the chick cecum compared to wild-type c. jejuni strain 81-176. predictions from genomic sequences identified cjj0382 as a second locu ... | 2008 | 18086814 |
| development and validation of an enzyme-linked immunosorbent assay for the diagnosis of porcine proliferative enteropathy. | the objective of this study was to develop an indirect enzyme-linked immunosorbent assay (elisa) using a sonicated pure culture of lawsonia intracellularis as the antigen (so-elisa). a total of 332 serum samples, consisting of 232 experimentally infected animals and 100 animals naturally infected with l. intracellularis, were used to assess the diagnostic sensitivity. three hundred and fifty-five sera from uninfected animals were used to determine the diagnostic specificity. the receiver operati ... | 2008 | 18319429 |
| evaluation of 16s rrna gene-based pcr assays for genus-level identification of helicobacter species. | the inclusivity, exclusivity, and detection limit of six 16s rrna gene-based helicobacter genus-specific pcr assays were examined. five out of six assays were 100% inclusive, but the tests varied considerably in their exclusivity (9.1 to 95.5%). the clinical detection limit varied between 10(3) and 1 viable bacterial cell per reaction mixture. | 2008 | 18337392 |
| enteric campylobacteria and rna viruses associated with healthy and diarrheic humans in the chinook health region of southwestern alberta, canada. | the presence of campylobacter species and enteric rna viruses in stools from diarrheic (n = 442) and healthy (n = 58) humans living in southwestern alberta was examined (may to october 2005). a large number of diarrheic individuals who were culture negative for c. jejuni (n = 54) or c. coli (n = 19) were pcr positive for these taxa. overall detection rates for c. jejuni and c. coli in diarrheic stools were 29% and 5%, respectively. in contrast, 3% and 0% of stools from healthy humans were positi ... | 2010 | 21106791 |
| enteric campylobacteria and rna viruses associated with healthy and diarrheic humans in the chinook health region of southwestern alberta, canada. | the presence of campylobacter species and enteric rna viruses in stools from diarrheic (n = 442) and healthy (n = 58) humans living in southwestern alberta was examined (may to october 2005). a large number of diarrheic individuals who were culture negative for c. jejuni (n = 54) or c. coli (n = 19) were pcr positive for these taxa. overall detection rates for c. jejuni and c. coli in diarrheic stools were 29% and 5%, respectively. in contrast, 3% and 0% of stools from healthy humans were positi ... | 2010 | 21106791 |
| a persistent and diverse airway microbiota present during chronic obstructive pulmonary disease exacerbations. | acute exacerbations of chronic obstructive pulmonary disease (copd) are a major source of morbidity and contribute significantly to healthcare costs. although bacterial infections are implicated in nearly 50% of exacerbations, only a handful of pathogens have been consistently identified in copd airways, primarily by culture-based methods, and the bacterial microbiota in acute exacerbations remains largely uncharacterized. the aim of this study was to comprehensively profile airway bacterial com ... | 2010 | 20141328 |
| three cases of severe invasive infections caused by campylobacter rectus and first report of fatal c. rectus infection. | we report the first fatal case of campylobacter rectus infection due to a subdural empyema and ruptured mycotic intracranial aneurysm and two cases of limb-threatening c. rectus necrotizing soft tissue and bone infection and empyema thoracis that responded to amoxicillin-clavulanate and surgical debridement and drainage. all three strains were identified by 16s rrna sequencing. | 2011 | 21270212 |
| automated identification of medically important bacteria by 16s rrna gene sequencing using a novel comprehensive database, 16spathdb. | despite the increasing use of 16s rrna gene sequencing, interpretation of 16s rrna gene sequence results is one of the most difficult problems faced by clinical microbiologists and technicians. to overcome the problems we encountered in the existing databases during 16s rrna gene sequence interpretation, we built a comprehensive database, 16spathdb (http://147.8.74.24/16spathdb) based on the 16s rrna gene sequences of all medically important bacteria listed in the manual of clinical microbiology ... | 2011 | 21389154 |
| emerging thermotolerant campylobacter species in healthy ruminants and swine. | campylobacters other than campylobacter jejuni or c. coli were isolated in 35% of 343 farms recently analyzed in northern spain. this study was aimed at identifying at the species level the 120 isolates collected (21 ovine, 52 beef cattle, 44 dairy cattle, and 3 porcine) by species-specific polymerase chain reaction and 16s rrna gene sequencing analysis. thus, five species were identified: campylobacter hyointestinalis (90 isolates), campylobacter lanienae (12), campylobacter fetus subsp. fetus ... | 2011 | 21438765 |
| multilocus sequence typing methods for the emerging campylobacter species c. hyointestinalis, c. lanienae, c. sputorum, c. concisus, and c. curvus. | multilocus sequence typing (mlst) systems have been reported previously for multiple food- and food animal-associated campylobacter species (e.g., c. jejuni, c. coli, c. lari, and c. fetus) to both differentiate strains and identify clonal lineages. these mlst methods focused primarily on campylobacters of human clinical (e.g., c. jejuni) or veterinary (e.g., c. fetus) relevance. however, other, emerging, campylobacter species have been isolated increasingly from environmental, food animal, or h ... | 2012 | 22919636 |
| occurrence and characteristics of fastidious campylobacteraceae species in porcine samples. | this study investigated the prevalence and characteristics of campylobacteraceae including a range of fastidious species in porcine samples. over a thirteen month period caecal contents (n=402) and pork carcass swabs (n=401) were collected from three pork abattoirs and pork products (n=399) were purchased at point of sale in the republic of ireland. campylobacteraceae isolates were recovered by enrichment, membrane filtration and incubation in antibiotic free media under a modified atmosphere (3 ... | 2013 | 23474652 |
| bacterial protein n-glycosylation: new perspectives and applications. | protein glycosylation is widespread throughout all three domains of life. bacterial protein n-glycosylation and its application to engineering recombinant glycoproteins continue to be actively studied. here, we focus on advances made in the last 2 years, including the characterization of novel bacterial n-glycosylation pathways, examination of pathway enzymes and evolution, biological roles of protein modification in the native host, and exploitation of the n-glycosylation pathways to create nov ... | 2013 | 23329827 |
| biological roles of the o-methyl phosphoramidate capsule modification in campylobacter jejuni. | campylobacter jejuni is a major cause of bacterial gastroenteritis worldwide, and the capsular polysaccharide (cps) of this organism is required for persistence and disease. c. jejuni produces over 47 different capsular structures, including a unique o-methyl phosphoramidate (meopn) modification present on most c. jejuni isolates. although the meopn structure is rare in nature it has structural similarity to some synthetic pesticides. in this study, we have demonstrated, by whole genome comparis ... | 2014 | 24498018 |
| multiplex detection of nine food-borne pathogens by mpcr and capillary electrophoresis after using a universal pre-enrichment medium. | routine microbiological quality analyses in food samples require, in some cases, an initial incubation in pre-enrichment medium. this is necessary in order to ensure that small amounts of pathogenic strains are going to be detected. in this work, a universal pre-enrichment medium has been developed for the simultaneous growth of bacillus cereus, campylobacter jejuni, clostridium perfringens, cronobacter sakazakii, escherichia coli, enterobacteriaceae family (38 species, 27 genera), listeria mono ... | 2015 | 26579100 |
| global epidemiology of campylobacter infection. | campylobacter jejuni infection is one of the most widespread infectious diseases of the last century. the incidence and prevalence of campylobacteriosis have increased in both developed and developing countries over the last 10 years. the dramatic increase in north america, europe, and australia is alarming, and data from parts of africa, asia, and the middle east indicate that campylobacteriosis is endemic in these areas, especially in children. in addition to c. jejuni, there is increasing rec ... | 2015 | 26062576 |
| the metabolically active bacterial microbiome of tonsils and mandibular lymph nodes of slaughter pigs. | the exploration of microbiomes in lymphatic organs is relevant for basic and applied research into explaining microbial translocation processes and understanding cross-contamination during slaughter. this study aimed to investigate whether metabolically active bacteria (mab) could be detected within tonsils and mandibular lymph nodes (mlns) of pigs. the hypervariable v1-v2 region of the bacterial 16s rrna genes was amplified from cdna from tonsils and mlns of eight clinically healthy slaughter p ... | 2015 | 26696976 |
| the use of pcr/electrospray ionization-time-of-flight-mass spectrometry (pcr/esi-tof-ms) to detect bacterial and fungal colonization in healthy military service members. | the role of microbial colonization in disease is complex. novel molecular tools to detect colonization offer theoretical improvements over traditional methods. we evaluated pcr/electrospray ionization-time-of-flight-mass spectrometry (pcr/esi-tof-ms) as a screening tool to study colonization of healthy military service members. | 2016 | 27448413 |