Publications

treponema saccharophilum sp. nov., a large pectinolytic spirochete from the bovine rumen.	a large, obligately anaerobic spirochete (strain pb) was isolated from bovine rumen fluid by a procedure involving rifampin as a selective agent. the helical cells measured 0.6 to 0.7 micron by 12 to 20 micron and possessed approximately 16 periplasmic flagella inserted near each end of the protoplasmic cylinder. the periplasmic flagella were arranged in a bundle wound around the cell body. strain pb utilized as fermentable substrates various plant polysaccharides (e.g., pectin, arabinogalactan, ...	1985	4051480
phylogenetic analysis of the spirochetes.	the 16s rrna sequences were determined for species of spirochaeta, treponema, borrelia, leptospira, leptonema, and serpula, using a modified sanger method of direct rna sequencing. analysis of aligned 16s rrna sequences indicated that the spirochetes form a coherent taxon composed of six major clusters or groups. the first group, termed the treponemes, was divided into two subgroups. the first treponeme subgroup consisted of treponema pallidum, treponema phagedenis, treponema denticola, a thermo ...	1991	1917844
assessment of the fructanolytic activities in the rumen bacterium treponema saccharophilum strain s.	to characterize the fructose polymer degrading enzymes of rumen bacterium treponema saccharophilum strain s.	2002	11849338
detection and quantification of oral treponemes in subgingival plaque by real-time pcr.	oral treponemes have been associated with periodontal diseases. we developed a highly sensitive and specific method to detect and quantify cultivable oral treponemes (treponema denticola, treponema vincentii, and treponema medium) in 50 subgingival plaque samples from 13 healthy subjects as well as 37 patients with periodontal diseases using real-time pcr assays with specific primers and a taqman probe for each 16s rrna sequence. the specificity for each assay was examined by using dna specimens ...	2002	12202575
spirochaeta coccoides sp. nov., a novel coccoid spirochete from the hindgut of the termite neotermes castaneus.	a novel spirochete strain, spn1, was isolated from the hindgut contents of the termite neotermes castaneus. the highest similarities (about 90%) of the strain spn1 16s rrna gene sequence are with spirochetes belonging to the genus spirochaeta, and thus, the isolate could not be assigned to the so-called termite clusters of the treponemes or to a known species of the genus spirochaeta. therefore, it represents a novel species, which was named spirochaeta coccoides. in contrast to all other known ...	2006	16391069
the macaque gut microbiome in health, lentiviral infection, and chronic enterocolitis.	the vertebrate gut harbors a vast community of bacterial mutualists, the composition of which is modulated by the host immune system. many gastrointestinal (gi) diseases are expected to be associated with disruptions of host-bacterial interactions, but relatively few comprehensive studies have been reported. we have used the rhesus macaque model to investigate forces shaping gi bacterial communities. we used dna bar coding and pyrosequencing to characterize 141,000 sequences of 16s rrna genes ob ...	2008	18248093
variability of treponemes in the rumen of ruminants.	complete 16s rrna sequences were determined of recently proposed new species of treponemes designated strain s and t. sequence comparison indicated that both species belong to the treponema saccharophilum cluster, having thus at least 5 cultivable representatives. phylogenetic analysis of available genbank 16s rrna sequences revealed two phylogenetically distant treponema clusters (t. saccharophilum cluster and t. bryantii cluster). surprisingly, while among cultivated treponemes dominate t. sac ...	2010	20680575
characterization of novel bovine gastrointestinal tract treponema isolates and comparison with bovine digital dermatitis treponemes.	this study aimed to isolate and characterize treponemes present in the bovine gastrointestinal (gi) tract and compare them with bovine digital dermatitis (bdd) treponemes. seven spirochete isolates were obtained from the bovine gi tract, which, on the basis of 16s rrna gene comparisons, clustered within the genus treponema as four novel phylotypes. one phylotype was isolated from several different gi tract regions, including the omasum, colon, rumen, and rectum. these four phylotypes could be di ...	2010	21057019
characterization of novel bovine gastrointestinal tract treponema isolates and comparison with bovine digital dermatitis treponemes.	this study aimed to isolate and characterize treponemes present in the bovine gastrointestinal (gi) tract and compare them with bovine digital dermatitis (bdd) treponemes. seven spirochete isolates were obtained from the bovine gi tract, which, on the basis of 16s rrna gene comparisons, clustered within the genus treponema as four novel phylotypes. one phylotype was isolated from several different gi tract regions, including the omasum, colon, rumen, and rectum. these four phylotypes could be di ...	2010	21057019
a phylogenomic and molecular signature based approach for characterization of the phylum spirochaetes and its major clades: proposal for a taxonomic revision of the phylum.	the spirochaetes species cause many important diseases including syphilis and lyme disease. except for their containing a distinctive endoflagella, no other molecular or biochemical characteristics are presently known that are specific for either all spirochaetes or its different families. we report detailed comparative and phylogenomic analyses of protein sequences from spirochaetes genomes to understand their evolutionary relationships and to identify molecular signatures for this group. these ...	2013	23908650
phylogenetic analysis of pbp genes in treponemes.	β-lactamases are the main cause of bacterial resistance to penicillin, cephalosporins, and related β-lactam compounds. the presence of the novel penicillin-binding protein (pbp) tp47 in treponema pallidum has been reported to be a well-known mechanism for turnover of b-lactam antibiotics. although, t. pallidum remains sensitive to penicillin, clinically significant resistance to macrolides has emerged in many developing countries. the genome sequence of t. pallidum has shown the presence of gene ...	2013	23330059
discovery of bovine digital dermatitis-associated treponema spp. in the dairy herd environment by a targeted deep-sequencing approach.	the bacteria associated with the infectious claw disease bovine digital dermatitis (dd) are spirochetes of the genus treponema; however, their environmental reservoir remains unknown. to our knowledge, the current study is the first report of the discovery and phylogenetic characterization of rrna gene sequences from dd-associated treponemes in the dairy herd environment. although the spread of dd appears to be facilitated by wet floors covered with slurry, no dd-associated treponemes have been ...	2014	24814794
monitoring the rumen pectinolytic bacteria treponema saccharophilum using real-time pcr.	treponema saccharophilum is a pectinolytic bacterium isolated from the bovine rumen. the abundance of this bacterium has not been well determined, reflecting the lack of a reliable and accurate detection method. to develop a rapid method for monitoring t. saccharophilum, we performed pyrosequencing of genomic dna isolated from rumen microbiota to explore the 16s rrna gene sequences of t. saccharophilum candidates. species-specific primers were designed based on fifteen sequences of partial 16s r ...	2014	24289046
first insights into the microbial diversity in the omasum and reticulum of bovine using illumina sequencing.	the digestive systems of mammals harbor a complex gut microbiome, comprising bacteria and other microorganisms that confer metabolic and immunological benefits to the host. ruminants that digest plant-based foods have a four-compartment stomach consisting of the rumen, reticulum, omasum, and abomasum. the microorganisms in the stomach are essential for providing the host with critical nutrients. however, the majority of these microorganisms are unknown species. the microbiome of the stomach is d ...	2015	25604266