Publications

[isolation of pure methanotrophic cultures and their properties].	pure cultures of obligate methanotrophic bacteria were isolated from natural habitats and cultivated on media containing silica gel which provided more elective conditions than media with agar. according to their morphology, fine structure, cultural, physiological and biochemical properties, the bacteria were identified as methylosinus trichosporium, methylosinus trichosporium var. methanolicum, methylocystis parvus, methylocytis parvus var. fuscus, methylomonas methanica.	1975	1107760
[isolation of bacteriophages of methane oxidizing bacteria and study of their properties].	five strains of bacteriophages were isolated for the first time in the ussr from the water of ponds, the paste of methane oxidizing bacteria and the cultural broth of the experimental plant. the strains are specific of the following species: methylostinus sporium, methylosinus trichosporium, and flavobacterium gasotypicum. bacteriophages lysing methylocystis impression. methylomonas agile and methylococcus capsulatus were not isolated so far. the fine structure of the phages, the shape of negati ...	1976	827665
[isolation and properties of new strains of obligate methanotrophs].	new strains of obligate methanotrophic bacteria which assimilate only methane or methanol as the source of carbon and energy have been isolated. according to their morphology, ultrastructure, cultural and physiologo-biochemical characteristics, the bacteria were classed as methylobacter vinelandii, methylobacter bovis, methylobacter chroococcum and mehylosinus sporium. a new species methylocystis echinoides sp. nov. is described; it differs from other methanotrophs in certain morphological and p ...	1977	600092
[formation of the taxa of methane-oxidizing bacteria by numerical analysis methods].	obligate methane oxidizing bacteria were classified within groups using numerical analysis and computer techniques. employment of properties coinciding for all the species of the bacterial group under study was found to result in an insignificant (5--10%) change in the degree of similarity rather than in the redistribution of interrelationship; it had no effect on the formation of phenons. the smirnov taxonomical analysis according to which the weight of a property is inversely proportional to i ...	1978	101743
[nomenclature of obligate methylotrophs].	the nomenclature of obligate methylotrophs, i. e. bacteria using only reduced monocarbon compounds (methane, methanol, methylamines) as a carbon source, is dicussed. the chronology of naming taxons of methane oxidizing bacteria is presented and the rightfulness of their names is analyzed according to the rules of the international codex of bacterial nomenclature. such names as methylomonas and others which are employed while describing various physiological groups of bacteria are used in the nom ...	1978	106220
copper ions cause cell elongation of methylocystis strains.		1979	121179
growth and polysaccharide production by methylocystis parvus obbp on methanol.	methylocystis parvus obbp, an obligate methylotroph originally isolated as a methane-utilizing bacterium, was cultivated on methanol as a sole source of carbon. after adaptation to high methanol levels, this organism grew on methanol with a maximum specific growth rate of 0.65 h. the ph optimum for growth was between 7 and 9, and the temperature optimum was between 30 and 37 degrees c. methanol concentrations higher than 5% (by weight) were toxic. formaldehyde, at a concentration greater than 1 ...	1979	16345377
[numerical analysis of the protein electrophoregrams of obligate methanotrophic bacteria].	the protein spectra for 70 strains of obligate methanotrophic bacteria were studied using electrophoresis in polyacrylamide gel with sodium dodecyl sulphate. the protein spectra of methylomonas methanica 12 and methylosinus trichosporium 44 did not change in the course of the culture growth. basing on the data obtained, the similarity coefficients were calculated for these strains. the numerical analysis of the similarity coefficients was done, and a dendrogram presenting the phylogenetic relati ...	1981	6799757
bacteriophages of methanotrophs isolated from fish.	bacteriophages of methanotrophic bacteria were isolated from 67 fish. only two phages isolated from two fish species specifically lysed methylocystis sp. and flavobacterium gasotypicum. the phages lysing these species were designated 63-f and cmf-1-f, respectively. the isolated phages differed greatly in the fine structure of the virion, plaque morphology, spectrum of lytic action, serological properties, and uv sensitivity. at the same time, they had identical one-step growth characteristics: t ...	1983	6416169
characteristics of a nitrogen-fixing methanotroph, methylocystis t-1.	a methane-oxidizing bacterium capable of nitrogen fixation was isolated from soil taken from an area which leaked methane gas. strain t-1 was a catalase and oxidase-positive, gram-negative straight rod-shaped strictly aerobic bacterium which formed lipid cysts and type ii intracytoplasmic membranes. the organism was a microaerophilic nitrogen-fixing methanotroph. strain t-1 is considered to be classified into methylocystis. the organism evolved hydrogen gas when grown in the nitrogen-free medium ...	1988	3148292
16s ribosomal rna sequence analysis for determination of phylogenetic relationship among methylotrophs.	16s ribosomal rnas (rrna) of 12 methylotrophic bacteria have been almost completely sequenced to establish their phylogenetic relationships. methylotrophs that are physiologically related are phylogenetically diverse and are scattered among the purple eubacteria (class proteobacteria). group i methylotrophs can be classified in the beta- and the gamma-subdivisions and group ii methylotrophs in the alpha-subdivision of the purple eubacteria, respectively. pink-pigmented facultative and non-pigmen ...	1990	1693657
screening for restriction endonucleases in methane-oxidizing bacteria.	51 methane-oxidizing bacteria strains such as methylomonas methanica, m. rubra, methylococcus capsulatus, m. thermophilus, m. luteus, m. ucrainicus, m. whittenburyi, methylosinus trichosporium, m. sporium, methylocystis parvus isolated from various ecological niches and geographical regions of the ukraine and also the strains received from r. whittenbury and y. heyer were screened for restriction endonucleases. type ii restriction endonucleases were detected in imv b-3112 (= 12 b), imv b-3027 (= ...	1992	1338116
role of heterotrophic bacteria in complete mineralization of trichloroethylene by methylocystis sp. strain m.	biodegradation experiments with radioactively labeled trichloroethylene showed that 32% of the radioactive carbon was converted to glyoxylic acid, dichloroacetic acid and trichloroacetic acid and that the same percentage was converted to co2 and co after 140 h of incubation by a pure culture of a type ii methane-utilizing bacterium, methylocystis sp. strain m, isolated from a mixed culture, mu-81, in our laboratory. in contrast, these water-soluble (14c)trichloroethylene degradation products wer ...	1992	1444420
characterization of the methanotrophic bacterial community present in a trichloroethylene-contaminated subsurface groundwater site.	groundwater, contaminated with trichloroethylene (tce) and tetrachloroethylene (pce), was collected from 13 monitoring wells at area m on the u.s. department of energy savannah river site near aiken, s.c. filtered groundwater samples were enriched with methane, leading to the isolation of 25 methanotrophic isolates. the phospholipid fatty acid profiles of all the isolates were dominated by 18:1 omega 8c (60 to 80%), a signature lipid for group ii methanotrophs. subsequent phenotypic testing show ...	1993	8368829
methanotrophic bacteria and facilitated transport of pollutants in aquifer material.	in situ stimulation of methanotrophic bacteria has been considered as a methodology for aquifer remediation. chlorinated aliphatic hydrocarbons such as trichloroethylene are fortuitously oxidized by the methane monooxygenase produced by methanotrophic bacteria. experimental results are presented that indicate that both colloidal suspensions containing methanotrophic cells and the soluble extracellular polymers produced by methanotrophic cells have the potential to enhance the transport and remov ...	1994	16349401
the soluble methane monooxygenase gene cluster of the trichloroethylene-degrading methanotroph methylocystis sp. strain m.	in methanotrophic bacteria, methane is oxidized to methanol by the enzyme methane monooxygenase (mmo). the soluble mmo enzyme complex from methylocystis sp. strain m also oxidizes a wide range of aliphatic and aromatic compounds, including trichloroethylene. in this study, heterologous dna probes from the type ii methanotroph methylosinus trichosporium ob3b were used to isolate souble mmo (smmo) genes from the type ii methanotroph methylocystis sp. strain m. smmo genes from strain m are clustere ...	1997	9143121
a secretory protein involved in the antagonistic interactions between methanotrophic bacteria.	antagonistic interactions in mixed culture of methanotrophic bacteria methylomonas methanica 12 and methylocystis minimus 33 were investigated. the inhibitory action of mcs. minimus exometabolites against mm. methanica grown in liquid medium was found to be specific. ultrafiltration established that the molecular weight of the substance having inhibitory activity lies within the range 2-10 kd. the activity is protease sensitive and relatively stable to heating. electrophoretic analysis showed th ...	1997	9275277
purification and properties of methanol dehydrogenase from methylocystis sp. gb 25.	methanol dehydrogenase (mdh) from methylocystis sp. gb 25, which belongs to the group ii of methanotrophic bacteria, is able to catalyse the oxidation of methanol to formate directly. the enzyme was purified 20-fold by a 5 step procedure to electrophoretic homogeneity. after cell disruption by french press, about 95% of mdh-activity was found in the soluble fraction. the relative molecular mass of the native enzyme has been estimated to be 122 kda by gel filtration and 115 kda by the method of h ...	1997	9323867
acidophilic methanotrophic communities from sphagnum peat bogs.	highly enriched methanotrophic communities (> 25 serial transfers) were obtained from acidic ombrotrophic peat bogs from four boreal forest sites. the enrichment strategy involved using media conditions that were associated with the highest rates of methane uptake by the original peat samples, namely, the use of diluted mineral medium of low buffering capacity, moderate incubation temperature (20 degrees c), and ph values of 3 to 6. enriched communities contained a mixture of rod-shaped bacteria ...	1998	9501432
purification and properties of methanol dehydrogenase from methylosinus sp. wi 14.	similarly to the recently described methanol dehydrogenase (mdh) from methylocystis sp. gb 25 (grosse et al. 1997) mdh from methylosinus sp. wi 14 is able to catalyse the oxidation of methanol to formate directly. the enzyme was purified about 9-fold to electrophoretic homogeneity and is localised in the soluble fraction. the relative molecular mass of the native enzyme has been determined to be 140 kda. it is composed of two identical subunits of relative molecular mass 70 kda. the amino termin ...	1998	9726124
methanotrophs and methanogens in masonry	methanotrophs were present in 48 of 225 stone samples which were removed from 19 historical buildings in germany and italy. the average cell number of methanotrophs was 20 cfu per g of stone, and their activities ranged between 11 and 42 pmol of ch4 g of stone-1 day-1. twelve strains of methane-oxidizing bacteria were isolated. they belonged to the type ii methanotrophs of the genera methylocystis, methylosinus, and methylobacterium. in masonry, growth substrates like methane or methanol are ava ...	1998	9797318
cytochrome p460 genes from the methanotroph methylococcus capsulatus bath.	p460 cytochromes catalyze the oxidation of hydroxylamine to nitrite. they have been isolated from the ammonia-oxidizing bacterium nitrosomonas europaea (r. h. erickson and a. b. hooper, biochim. biophys. acta 275:231-244, 1972) and the methane-oxidizing bacterium methylococcus capsulatus bath (j. a. zahn et al., j. bacteriol. 176:5879-5887, 1994). a degenerate oligonucleotide probe was synthesized based on the n-terminal amino acid sequence of cytochrome p460 and used to identify a dna fragment ...	1998	9851984
characterization of root-associated methanotrophs from three freshwater macrophytes: pontederia cordata, sparganium eurycarpum, and sagittaria latifolia.	root-associated methanotrophic bacteria were enriched from three common aquatic macrophytes: pontederia cordata, sparganium eurycarpum, and sagittaria latifolia. at least seven distinct taxa belonging to groups i and ii were identified and presumptively assigned to the genera methylosinus, methylocystis, methylomonas, and methylococcus. four of these strains appeared to be novel on the basis of partial 16s ribosomal dna sequence analysis. the root-methanotroph association did not appear to be hi ...	1998	16349515
effect of temperature and pressure on growth and methane utilization by several methanotrophic cultures.	several methanotrophic microorganisms, i.e., methylococcus capsulatus (bath), methylomonas albus (bg-8), methylosinus trichosporium ob3b, and methylocystis parvus (obbp), were evaluated for growth and methane utilization. the effect of temperature was examined in the range of 25 to 45 degrees c for growth and methane utilization. the temperature variations (25-35 degrees c) had minimal effect on growth of m. albus and m. parvus. methane consumption varied at different temperatures with a maximum ...	1998	18576037
high-affinity methane oxidation by a soil enrichment culture containing a type ii methanotroph.	methanotrophic bacteria in an organic soil were enriched on gaseous mixing ratios of <275 parts per million of volume (ppmv) of methane (ch4). after 4 years of growth and periodic dilution (>10(20) times the initial soil inoculum), a mixed culture was obtained which displayed an apparent half-saturation constant [km(app)] for ch4 of 56 to 186 nm (40 to 132 ppmv). this value was the same as that measured in the soil itself and about 1 order of magnitude lower than reported values for pure culture ...	1999	10049856
induction and enhancement of stress proteins in a trichloroethylene-degrading methanotrophic bacterium, methylocystis sp. m.	the responses of the trichloroethylene-degrading bacterium methylocystis sp. m to six different water-pollutants, carbon starvation, and temperature-shock (heat and cold) were examined using 2-dimensional gel electrophoresis. twenty-eight polypeptides were induced, and these stress-induced proteins were classified into three groups. some of the chemically induced proteins were the same as those induced by carbon starvation and temperature-shock. two of the polypeptides were induced by trichloroe ...	1999	10094607
molecular analyses of the methane-oxidizing microbial community in rice field soil by targeting the genes of the 16s rrna, particulate methane monooxygenase, and methanol dehydrogenase	rice field soil with a nonsaturated water content induced ch4 consumption activity when it was supplemented with 5% ch4. after a lag phase of 3 days, ch4 was consumed rapidly until the concentration was less than 1.8 parts per million by volume (ppmv). however, the soil was not able to maintain the oxidation activity at near-atmospheric ch4 mixing ratios (i.e., 5 ppmv). the soil microbial community was monitored by performing denaturing gradient gel electrophoresis (dgge) during the oxidation pr ...	1999	10223989
heterologous expression of soluble methane monooxygenase genes in methanotrophs containing only particulate methane monooxygenase.	the methanotrophs methylococcus capsulatus (bath) and methylosinus trichosporium ob3b contain particulate methane monooxygenase (pmmo) and soluble methane monooxygenase (smmo) genes. other methanotrophs such as methylomicrobium album bg8 and methylocystis parvus obbp contain only pmmo genes. although molecular genetic techniques are poorly developed in methanotrophs, smmo genes were expressed in methanotrophs normally containing only pmmo genes. this was achieved by conjugation using broad-host- ...	1999	10369892
purification and characterization of the soluble methane monooxygenase of the type ii methanotrophic bacterium methylocystis sp. strain wi 14.	methane monooxygenase (mmo) catalyzes the oxidation of methane to methanol as the first step of methane degradation. a soluble nad(p)h-dependent methane monooxygenase (smmo) from the type ii methanotrophic bacterium wi 14 was purified to homogeneity. sequencing of the 16s rdna and comparison with that of other known methanotrophic bacteria confirmed that strain wi 14 is very close to the genus methylocystis. the smmo is expressed only during growth under copper limitation (<0.1 microm) and with ...	1999	10473397
methanotroph diversity in landfill soil: isolation of novel type i and type ii methanotrophs whose presence was suggested by culture-independent 16s ribosomal dna analysis.	the diversity of the methanotrophic community in mildly acidic landfill cover soil was assessed by three methods: two culture-independent molecular approaches and a traditional culture-based approach. for the first of the molecular studies, two primer pairs specific for the 16s rrna gene of validly published type i (including the former type x) and type ii methanotrophs were identified and tested. these primers were used to amplify directly extracted soil dna, and the products were used to const ...	1999	10543800
molecular characterization of functional and phylogenetic genes from natural populations of methanotrophs in lake sediments.	the 16s rrna and pmoa genes from natural populations of methane-oxidizing bacteria (methanotrophs) were pcr amplified from total community dna extracted from lake washington sediments obtained from the area where peak methane oxidation occurred. clone libraries were constructed for each of the genes, and approximately 200 clones from each library were analyzed by using restriction fragment length polymorphism (rflp) and the tetrameric restriction enzymes mspi, haeiii, and hhai. the pcr products ...	1999	10543824
soluble methane monooxygenase gene clusters from trichloroethylene-degrading methylomonas sp. strains and detection of methanotrophs during in situ bioremediation.	the soluble mmo (smmo) gene clusters from group i methanotrophs were characterized. an 8.1-kb kpni fragment from methylomonas sp. strain kswiii and a 7.5-kb sali fragment from methylomonas sp. strain kspiii which contained the smmo gene clusters were cloned and sequenced. the sequences of these two fragments were almost identical. the smmo gene clusters in the fragment consisted of six open reading frames which were 52 to 79% similar to the corresponding genes of previously described smmo gene c ...	1999	10583965
molecular analysis of the pmo (particulate methane monooxygenase) operons from two type ii methanotrophs.	the particulate methane monooxygenase gene clusters, pmocab, from two representative type ii methanotrophs of the alpha-proteobacteria, methylosinus trichosporium ob3b and methylocystis sp. strain m, have been cloned and sequenced. primer extension experiments revealed that the pmo cluster is probably transcribed from a single transcriptional start site located 300 bp upstream of the start of the first gene, pmoc, for methylocystis sp. strain m. immediately upstream of the putative start site, c ...	2000	10698759
methylocella palustris gen. nov., sp. nov., a new methane-oxidizing acidophilic bacterium from peat bogs, representing a novel subtype of serine-pathway methanotrophs.	a new genus, methylocella, and a new species, methylocella palustris, are proposed for three strains of methane-oxidizing bacteria isolated from acidic sphagnum peat bogs. these bacteria are aerobic, gram-negative, colourless, non-motile, straight and curved rods that utilize the serine pathway for carbon assimilation, multiply by normal cell division and contain intracellular poly-beta-hydroxybutyrate granules (one at each pole). these strains use methane and methanol as sole sources of carbon ...	2000	10843033
[characteristics of hemagglutination reaction in methanotrophic bacteria].	the reaction of hemagglutination with trypsin-treated rabbit erythrocytes was used to reveal lectins on the cell surface of methanotrophic bacteria and in their culture liquids. by this method, no lectins were detected on the cell surface of methylococcus capsulatus imv b-3001 and methylomonas rubra imv b-3075 or in the culture liquid of any of the species studied. with intact cells of methylocystis parvus imv b-3491, the positive hemagglutination reaction observed was nonspecific and most proba ...	2000	10920812
starvation alters the apparent half-saturation constant for methane in the type ii methanotroph methylocystis strain lr1.	when cells of a type ii methanotrophic bacterium (methylocystis strain lr1) were starved of methane, both the k(m(app)) and the v(max(app)) for methane decreased. the specific affinity (a(o)(s)) remained nearly constant. therefore, the decreased k(m(app)) in starved cells was probably not an adjustment to better utilize low-methane concentrations.	2000	10966442
polyclonal antibodies recognizing the amob protein of ammonia oxidizers of the beta-subclass of the class proteobacteria.	a 41-kda protein of nitrosomonas eutropha was purified, and the n-terminal amino acid sequence was found to be nearly identical with the sequence of amob, a subunit of ammonia monooxygenase. this protein was used to develop polyclonal antibodies, which were highly specific for the detection of the four genera of ammonia oxidizers of the beta-subclass of proteobacteria (nitrosomonas, including nitrosococcus mobilis, which belongs phylogenetically to nitrosomonas; nitrosospira; nitrosolobus; and n ...	2001	11133435
producing poly-3-hydroxybutyrate with a high molecular mass from methane.	poly-3-hydroxybutyrate (phb) and other polyesters can be produced by various species of bacteria. of the possible carbon sources, methane could provide a suitable substrate for the production of phb. methane is cheap and plentiful - not only as natural gas, but also as biogas. the methanotrophic strain methylocystis sp. gb 25 dsmz 7674 is able to accumulate phb in a brief non-sterile process. the studies were carried out using a 7-l and a 70-l pressure bioreactor. cultivation was performed in tw ...	2001	11245901
improved method for detection of methanotrophic bacteria in forest soils by pcr.	a primer set was designed for the specific detection of methanotrophic bacteria in forest soils by pcr. the primer sequences were derived from highly conservative regions of the pmoa gene, encoding the alpha-subunit of the particulate methane monooxygenase present in all methanotrophs. in control experiments with genomic dna from a collection of different type i, ii, and x methanotrophs, it could be demonstrated that the new primers were specific for members of the genera methylosinus, methylocy ...	2001	11400051
methane oxidation and the competition for oxygen in the rice rhizosphere.	a mechanistic approach is presented to describe oxidation of the greenhouse gas methane in the rice rhizosphere of flooded paddies by obligate methanotrophic bacteria. in flooded rice paddies these methanotrophs compete for available o(2) with other types of bacteria. soil incubation studies and most-probable-number (mpn) counts of oxygen consumers show that microbial oxygen consumption rates were dominated by heterotrophic and methanotrophic respiration. mpn counts of methanotrophs showed large ...	2001	11472935
detection of methanotroph diversity on roots of submerged rice plants by molecular retrieval of pmoa, mmox, mxaf, and 16s rrna and ribosomal dna, including pmoa-based terminal restriction fragment length polymorphism profiling.	the diversity of methanotrophic bacteria associated with roots of submerged rice plants was assessed using cultivation-independent techniques. the research focused mainly on the retrieval of pmoa, which encodes the alpha subunit of the particulate methane monooxygenase. a novel methanotroph-specific community-profiling method was established using the terminal restriction fragment length polymorphism (t-rflp) technique. the t-rflp profiles clearly revealed a more complex root-associated methanot ...	2001	11526021
[growth of mesophilic methanotrophs at low temperatures].	the optimal growth of mesophilic methanotrophic bacteria (collection strains of the genera methylocystis, methylomonas, methylosinus, and methylobacter) occurred within temperature ranges of 31-34 degrees c and 23-25 degrees c. none of the strains studied were able to grow at 1.5 or 4 degrees c. representatives of six methanotrophic species (strains mcs. echinoides 2, mm. methanica 12, mb. bovis 89, mcs. pyriformis 14, mb. chroococcum 90, and mb. vinelandii 87) could grow at 10 degrees c (with a ...	2001	11558268
family- and genus-level 16s rrna-targeted oligonucleotide probes for ecological studies of methanotrophic bacteria.	methanotrophic bacteria play a major role in the global carbon cycle, degrade xenobiotic pollutants, and have the potential for a variety of biotechnological applications. to facilitate ecological studies of these important organisms, we developed a suite of oligonucleotide probes for quantitative analysis of methanotroph-specific 16s rrna from environmental samples. two probes target methanotrophs in the family methylocystaceae (type ii methanotrophs) as a group. no oligonucleotide signatures t ...	2001	11571178
detection and enumeration of methanotrophs in acidic sphagnum peat by 16s rrna fluorescence in situ hybridization, including the use of newly developed oligonucleotide probes for methylocella palustris.	two 16s rrna-targeted oligonucleotide probes, mcell-1026 and mcell-181, were developed for specific detection of the acidophilic methanotroph methylocella palustris using fluorescence in situ hybridization (fish). the fluorescence signal of probe mcell-181 was enhanced by its combined application with the oligonucleotide helper probe h158. mcell-1026 and mcell-181, as well as 16s rrna oligonucleotide probes with reported group specificity for either type i methanotrophs (probes m-84 and m-705) o ...	2001	11571193
[search for methanotrophic producers of exopolysaccharides].	bacteria that produce exopolysaccharides (eps) and use methane as the only source of carbon were selected by studying a collection of methanotroph strains: methylococcus capsulatus e 494, 874, and 3009; m. thermophilus 111p, 112p, and 119p; methylobacter ucrainicus 159 and 161; m. luteus 57v and 12b; methylobacter sp. 100; methylomonas rubra 15 sh and sk-32; methylosinus trichosporium ov3b, ov5b and 4e; m. sporium 5, 12, a20d, and 90v; and methylocystis parvus ovvp. mesophilic methanotroph strai ...	2001	11771325
quantitative and specific detection of a trichloroethylene-degrading methanotroph, methylocystis sp. strain m, by a most probable number-polymerase chain reaction method.	we developed a rapid and specific enumeration method for a trichloroethylene-degrading methanotroph, methylocystis sp. strain m, based on a most probable number-polymerase chain reaction method for monitoring the bacterium at bioremediation sites. the primers designed for the mmoc gene of the soluble methane monooxygenase gene cluster were specific to strain m. recovery of the cells with a membrane filter enabled us to detect strain m in trichloroethylene-contaminated groundwater. we used the en ...	2001	11826963
the ribulose-1,5-bisphosphate carboxylase/oxygenase gene cluster of methylococcus capsulatus (bath).	the genes encoding the ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) from methylococcus capsulatus (bath) were localised to an 8.3-kb ecori fragment of the genome. genes encoding the large subunit ( cbbl), small subunit ( cbbs) and putative regulatory gene ( cbbq) were shown to be located on one cluster. surprisingly, cbbo, a second putative regulatory gene, was not located in the remaining 1.2-kb downstream (3') of cbbq. however, probing of the m. capsulatus (bath) genome with cbbo ...	2002	11889481
detection of methanotrophs with highly divergent pmoa genes from arctic soils.	tundra soil samples from the canadian arctic community, kuujjuaq, were analyzed for the presence of the soluble (smmo) and particulate (pmmo) methane monooxygenase genes. total genomic dna extracted from these soils was used as template for pcr using smmo- and pmmo-specific primers, mmox1-mmox2 and a189-a682, respectively. pmmo and smmo genes were detected in the kuujjuaq soil samples. isolation of smmo-possessing methanotrophic microorganisms from the three soils, as determined by the colony na ...	2002	12007824
[methanotrophic communities in the soils of russian northern taiga and subarctic tundra].	the pcr analysis of dna extracted from soil samples taken in russian northern taiga and subarctic tundra showed that the dna extracts contain genes specific to methanotrophic bacteria, i.e., the mmox gene encoding the conserved alpha-subunit of the hydroxylase component of soluble methane monooxygenase, the pmoa gene encoding the alpha-subunit of particulate methane monooxygenase, and the mxaf gene encoding the alpha-subunit of methanol dehydrogenase. pcr analysis with group-specific primers als ...	2002	12024830
identification of active methylotroph populations in an acidic forest soil by stable-isotope probing.	stable-isotope probing (sip) is a culture-independent technique that enables the isolation of dna from micro-organisms that are actively involved in a specific metabolic process. in this study, sip was used to characterize the active methylotroph populations in forest soil (ph 3.5) microcosms that were exposed to (13)ch(3)oh or (13)ch(4). distinct (13)c-labelled dna ((13)c-dna) fractions were resolved from total community dna by cscl density-gradient centrifugation. analysis of 16s rdna sequence ...	2002	12177327
[effect of gas mixture component composition on the process of hard materials colonization by methanotrophic bacteria].	a device has been proposed which allows decreasing a possibility to contaminate the inoculum of methane-using bacteria (methanotrophs) under its growing. the gas mixture composition has been investigated for the intensity of grass and polysterene colonization by two species of methanotrophs: methylococcus capsulatus ucm b-3030, possessing hydrophilic surface and hydrophobic methylocystis parvus ukm b-3490t. it has been shown that immobilized methanotrophic bacteria can colonize the above materia ...	2002	12190038
molecular phylogeny of type ii methane-oxidizing bacteria isolated from various environments.	type ii methane-oxidizing bacteria (mob) were isolated from diverse environments, including rice paddies, pristine and polluted freshwaters and sediments, mangrove roots, upland soils, brackish water ecosystems, moors, oil wells, water purification systems and livestock manure. isolates were identified based on morphological traits as either methylocystis spp., methylosinus sporium or methylosinus trichosporium. molecular phylogenies were constructed based on nearly complete 16s rrna gene sequen ...	2002	12213929
quantitative and rapid detection of the trichloroethylene-degrading bacterium methylocystis sp. m in groundwater by real-time pcr.	we developed a method based on real-time pcr for the specific and rapid enumeration of a trichloroethylene-degrading methanotroph, methylocystis sp. m, with the aim of monitoring the strain in groundwater. a primer set designed from the nucleotide sequence of the mmoc gene of a soluble methane monooxygenase (smmo) gene cluster from methylocystis sp. m was specific to amplify the dna region from the strain and no pcr products were amplified with the smmo gene clusters from six other methanotroph ...	2002	12226733
[study of nucleotide sequences of nifh genes in methanotrophic bacteria].	using a previously developed primer system, nifh gene fragments 450 nucleotides long were amplified, cloned, and sequenced for representatives of nitrogen-fixing methanotrophic bacteria of the genera methylococcus, methylocystis and methylosinus. fragments of nifh genes were also detected and sequenced in representatives of the genera methylomonas and methylobacter, which were not considered diazotrophs until recently. phylogenetic analysis revealed remoteness of nifh genes sequences of methanot ...	2002	12244720
diversity of the particulate methane monooxygenase gene in methanotrophic samples from different rice field soils in china and the philippines.	methanotrophic bacteria play a crucial role in regulating the emission of ch4 from rice fields into the atmosphere. we investigated the ch4 oxidation activity together with the diversity of methanotrophic bacteria in ten rice field soils from different geographic locations. upon incubation of aerated soil slurries under 7% ch4, rates of ch4 oxidation increased after a lag phase of 1-4 days and reached values of 3-10 micromol d(-1) g-dw(-1) soil. the methanotrophic community was assayed by retrie ...	2002	12353882
[utilization of methane and carbon dioxide by symbiotrophic bacteria in gills of mytilidae (bathymodiolus) from the rainbow and logachev hydrothermal fields on the mid-atlantic ridge].	bivalve mollusks bathymodiolus asoricus and bathymodiolus puteoserpentis collected from the rainbow and logachev hydrothermal fields during dives of mir 1 and mir 2 deep-sea manned submersibles were studied. rates of methane oxidation and carbon dioxide assimilation in mussel gill tissue were determined by radiolabel analysis. during oxidation of 14ch4, radiocarbon was detected in significant quantities not only in carbon dioxide but also in dissolved organic matter, most notably 14c-formate and ...	2002	12449636
activity and community structure of methane-oxidising bacteria in a wet meadow soil.	the structure and activity of the methane-oxidising microbial community in a wet meadow soil in germany were investigated using biogeochemical, cultivation, and molecular fingerprinting techniques. both methane from the atmosphere and methane produced in anaerobic subsurface soil were oxidised. the specific affinity (first-order rate constant) for methane consumption was highest in the top 20 cm of soil and the apparent half-saturation constant was 137-300 nm ch(4), a value intermediate to measu ...	2002	19709259
isolation of a methylocystis strain containing a novel pmoa-like gene.	abstract a type ii methanotrophic bacterium (methylocystis strain sc2) was isolated from a polluted aquifer and identified based on morphology and on 16s rrna gene phylogeny. primers targeting the particulate methane monooxygenase subunit a gene (pmoa) were used to obtain a pcr product from dna extract of strain sc2. denaturing gradient gel electrophoresis of this pcr product demonstrated that strain sc2 contained two very different pmoa-like genes. one gene (pmoa1) had very high sequence homolo ...	2002	19709235
[methanotrophic bacteria of acid sphagnum bogs].	acid sphagnum bogs cover a considerable part of the territory of russia and are an important natural source of biogenic methane, which is formed in their anaerobic layers. a considerable portion of this methane is consumed in the aerobic part of the bog profile by acidophilic methanotrophic bacteria, which comprise the methane filter of sphagnum bogs and decrease ch4 emission to the atmosphere. for a long time, these bacteria escaped isolation, which became possible only after the elucidation of ...	2002	12526194
characterization of methanogenic and methanotrophic assemblages in landfill samples.	a greater understanding of the tightly linked trophic groups of anaerobic and aerobic bacteria residing in municipal solid waste landfills will increase our ability to control methane emissions and pollutant fate in these environments. to this end, we characterized the composition of methanogenic and methanotrophic bacteria in samples taken from two regions of a municipal solid waste landfill that varied in age. a method combining polymerase chain reaction amplification, restriction fragment len ...	2003	14667383
differential detection of type ii methanotrophic bacteria in acidic peatlands using newly developed 16s rrna-targeted fluorescent oligonucleotide probes.	abstract based on an extensive 16s rrna sequence database for type ii methanotrophic bacteria, a set of 16s rrna-targeted oligonucleotide probes was developed for differential detection of specific phylogenetic groups of these bacteria by fluorescence in situ hybridisation (fish). this set of oligonucleotides included a genus-specific probe for methylocystis (mcyst-1432) and three species-specific probes for methylosinus sporium (msins-647), methylosinus trichosporium (msint-1268) and the recent ...	2003	19719661
screening for soluble methane monooxygenase in methanotrophic bacteria using combined molecular and biochemical methods for hydroxylase detection.	three well known methanotrophic bacteria (methylosinus trichosporium ob3b, methylocystis sp. wi 14, and methylocystis sp. gb 25) and three newly isolated methanotrophic bacteria (methylocystis sp. wi 11, methylocystis sp. x, and fi-9) were screened for smmo considering the existence of hydroxylase (component a) genes as well as its gene expression. for these purposes monoclonal antibodies that specifically recognize each subunit of the hydroxylase of methylocystis sp. wi 14 (alpha-subunit [9e5/f ...	2003	12596237
cometabolic degradation of chlorinated aromatic compounds.	the degradation of chlorobenzene was investigated with the specially chosen strain methylocystis sp. gb 14 dsm 12955, using 23 ml headspace vials and in a soil column filled with quaternary aquifer material from a depth of 20 m. a long-term experiment was carried out in this column, situated in a mobile test unit at a contaminated location in bitterfeld (germany). groundwater polluted by chlorobenzene was continuously fed through the column, through which a mixture comprising 4% ch(4) and 96% ai ...	2003	12668318
amplification of marine methanotrophic enrichment dna with 16s rdna pcr primers for type ii alpha proteobacteria methanotrophs.	type ii alpha proteobacteria methanotrophs are capable of a wide range of cometabolic transformations of chlorinated solvents and polycyclic aromatic hydrocarbons (pahs), and this activity has been exploited in many terrestrial bioremediation systems. however, at present, all known obligately marine methanotrophic isolates are type i gamma proteobacteria which do not have this activity to the extent of type ii methanotrophs. in previous work in our laboratory, determining the presence of type ii ...	2003	12940489
wide distribution of a novel pmoa-like gene copy among type ii methanotrophs, and its expression in methylocystis strain sc2.	experiments were conducted to determine if a novel pmoa-like gene (pmoa2) recently discovered in the methane-oxidizing bacterium methylocystis strain sc2 (p. f. dunfield, m. tchawa yimga, s. d. dedysh, u. berger, w. liesack, and j. heyer, fems microbiol. ecol. 41:17-26, 2002) is present in other methane-oxidizing bacteria (mob), and if it is expressed. a newly developed primer combination (pmoa206f-pmoa703b) allowed a differential detection of pmoa1 and pmoa2. by using this primer combination, w ...	2003	12957949
diversity and activity of methanotrophic bacteria in different upland soils.	samples from diverse upland soils that oxidize atmospheric methane were characterized with regard to methane oxidation activity and the community composition of methanotrophic bacteria (mb). mb were identified on the basis of the detection and comparative sequence analysis of the pmoa gene, which encodes a subunit of particulate methane monooxygenase. mb commonly detected in soils were closely related to methylocaldum spp., methylosinus spp., methylocystis spp., or the "forest sequence cluster" ...	2003	14602631
optimization of diagnostic microarray for application in analysing landfill methanotroph communities under different plant covers.	landfill sites are responsible for 6-12% of global methane emission. methanotrophs play a very important role in decreasing landfill site methane emissions. we investigated the methane oxidation capacity and methanotroph diversity in lysimeters simulating landfill sites with different plant vegetations. methane oxidation rates were 35 g methane m-2 day-1 or higher for planted lysimeters and 18 g methane m-2 day-1 or less for bare soil controls. best methane oxidation, as displayed by gas depth p ...	2004	15008813
comparative analysis of the conventional and novel pmo (particulate methane monooxygenase) operons from methylocystis strain sc2.	in addition to the conventional pmoa gene (pmoa1) encoding the active site polypeptide of particulate methane monooxygenase, a novel pmoa gene copy (pmoa2) is widely distributed among type ii methanotrophs (methane-oxidizing bacteria [mob]) (m. tchawa yimga, p. f. dunfield, p. ricke, j. heyer, and w. liesack, appl. environ. microbiol. 69:5593-5602, 2003). here we report that the pmoa1 and pmoa2 gene copies in the type ii mob methylocystis strain sc2 are each part of a complete pmocab gene cluste ...	2004	15128567
[features of solid materials' colonization by pure and mixed cultures of methanotrophs].	the process of colonization of hydrophilic (glass) and hydrophobic (polysterene) carriers by pure cultures of methanotrophs methylocystis parvus ucm b-3490t, methylococcus capsulatus ucm b-3030, as well as by their cultures mixed with bacillus megaterium ucm b 5723t and pseudomonas putida vkpm b-4188 under the conditions efficient for methanotrophic bacteria. m. parvus demonstrated the highest intensity of this process on the above carriers owing to high hydrophobic cell surface. both methanotro ...	2004	15456220
[methanotrophs and methylobacteria are found in woody plant tissues within a winter period].	samples of tree seeds, buds, and needles collected within a winter period at ambient temperatures from -11 to -17 degrees c were analyzed for the presence of methylotrophic microflora. thin sections of blue spruce needles were found to contain bacteria morphologically close to pink-pigmented methylobacteria. the methylobacteria that were isolated in pure cultures from samples of linden seeds and buds, pine and blue spruce needles, as well as of lilac, maple, and apple buds, were classified into ...	2004	15688941
molecular phylogeny of methylotrophs in a deep-sea sediment from a tropical west pacific warm pool.	the presence and phylogeny of methylotrophs, including methanotrophs, in a deep-sea sediment of a tropical west pacific warm pool site wp was investigated by molecular marker-based analysis of mxaf, pmoa and archaeal 16s rrna genes. mxaf amino acid sequence analysis revealed that microbes belonging to the alpha-proteobacteria and most related to hyphomicrobium and methylobacterium were the dominant aerobic methylotrophs in this deep-sea sediment; also, a small percentage of type ii methanotrophs ...	2004	19712348
new dgge strategies for the analyses of methanotrophic microbial communities using different combinations of existing 16s rrna-based primers.	methane-oxidising microbial communities are studied intensively because of their importance for global methane cycling. a suite of molecular microbial techniques has been applied to the study of these communities. denaturing gradient gel electrophoresis (dgge) is a diversity screening tool combining high sample throughput with phylogenetic information of high resolution. the existing 16s rrna-based dgge assays available for methane-oxidising bacteria suffer from low-specificity, low phylogentic ...	2004	16329903
preferential cultivation of type ii methanotrophic bacteria from littoral sediments (lake constance).	most widely used medium for cultivation of methanotrophic bacteria from various environments is that proposed in 1970 by whittenbury. in order to adapt and optimize medium for culturing of methanotrophs from freshwater sediment, media with varying concentrations of substrates, phosphate, nitrate, and other mineral salts were used to enumerate methanotrophs by the most probable number method. high concentrations (>1 mm) of magnesium and sulfate, and high concentrations of nitrate (>500 microm) si ...	2004	19712333
utility of environmental primers targeting ancient enzymes: methylotroph detection in lake washington.	methods have been explored for detection of methylotrophs in natural samples, using environmental primers based on genes involved in the tetrahydromethanopterin (h4mpt)-linked c1 transfer pathway. the underlying hypotheses were that the h4mpt-linked pathway is an ancient methylotrophy pathway, based on gene divergence, and that primers targeting more divergent genes will detect a broader variety of methylotrophs compared to the variety uncovered using probes and primers targeting highly conserve ...	2004	15696380
analysis of methanotrophic bacteria in movile cave by stable isotope probing.	movile cave is an unusual groundwater ecosystem that is supported by in situ chemoautotrophic production. the cave atmosphere contains 1-2% methane (ch4), although much higher concentrations are found in gas bubbles that keep microbial mats afloat on the water surface. as previous analyses of stable carbon isotope ratios have suggested that methane oxidation occurs in this environment, we hypothesized that aerobic methane-oxidizing bacteria (methanotrophs) are active in movile cave. to identify ...	2004	14756876
nifh and nifd phylogenies: an evolutionary basis for understanding nitrogen fixation capabilities of methanotrophic bacteria.	the ability to utilize dinitrogen as a nitrogen source is an important phenotypic trait in most currently known methanotrophic bacteria (mb). this trait is especially important for acidophilic mb, which inhabit acidic oligotrophic environments, highly depleted in available nitrogen compounds. phylogenetically, acidophilic mb are most closely related to heterotrophic dinitrogen-fixing bacteria of the genus beijerinckia: to further explore the phylogenetic linkage between these metabolically diffe ...	2004	15133093
diversity of methanotroph communities in a basalt aquifer.	methanotrophic bacteria play an important role in global cycling of carbon and co-metabolism of contaminants. methanotrophs from pristine regions of the snake river plain aquifer (srpa; idaho, usa) were studied in order to gain insight into the native groundwater communities' genetic potential to carry out tce co-metabolism. wells were selected that were proximal to a tce plume believed to be undergoing natural attenuation. methane concentrations ranged from 1 to >1000 nm. carbon isotope ratios ...	2004	19712303
degradation of organic pollutants by methane grown microbial consortia.	microbial consortia were enriched from various environmental samples with methane as the sole carbon and energy source. selected consortia that showed a capacity for co-oxidation of naphthalene were screened for their ability to degrade methyl-tert-butyl-ether (mtbe), phthalic acid esters (pae), benzene, xylene and toluene (btx). mtbe was not removed within 24 h by any of the consortia examined. one consortium enriched from activated sludge ("aae-a2"), degraded pae, including (butyl-benzyl)phtha ...	2005	15865157
possibilities for controlling a phb accumulation process using various analytical methods.	poly-beta-hydroxybutyrate (phb) and other polyesters can be produced by various species of bacteria. of the possible carbon sources, methane could prove to be one of the most suitable substrates for the manufacture of phb. the methanotrophic strain methylocystis sp. gb 25 dsm 7674 was applied in order to accumulate phb in a rapid, non-sterile process. cultivation was performed in two stages: a continuous growth phase (dilution rate 0.17 h(-1)) and a phb accumulation phase under deficiency condit ...	2005	15831253
diversity of oxygenase genes from methane- and ammonia-oxidizing bacteria in the eastern snake river plain aquifer.	pcr amplification, restriction fragment length polymorphism, and phylogenetic analysis of oxygenase genes were used for the characterization of in situ methane- and ammonia-oxidizing bacteria from free-living and attached communities in the eastern snake river plain aquifer. the following three methane monooxygenase (mmo) pcr primer sets were used: a189-a682, which amplifies an internal region of both the pmoa gene of the mmo particulate form and the amoa gene of ammonia monooxygenase; a189-mb66 ...	2005	15812034
response and adaptation of different methanotrophic bacteria to low methane mixing ratios.	described genera of methanotrophic bacteria are present in most upland soils, but it is not known whether these are sufficiently oligotrophic to oxidize methane at its trace atmospheric mixing ratio of 1.75 ppmv. members of the genera methylocystis, methylosinus, methylocaldum and methylobacter were isolated from different upland soils and compared with type strains for growth and activity under low methane mixing ratios. the specific affinity (a0s) varied by about one order of magnitude among d ...	2005	16104854
diversity of methanotrophic bacteria in tropical upland soils under different land uses.	three upland soils from thailand, a natural forest, a 16-year-old reforested site, and an agricultural field, were studied with regard to methane uptake and the community composition of methanotrophic bacteria (mb). the methane uptake rates were similar to rates described previously for forest and farmland soils of the temperate zone. the rates were lower at the agricultural site than at the native forest and reforested sites. the sites also differed in the mb community composition, which was ch ...	2005	16000794
[physicochemical and biological factors affecting atmospheric methane oxidation in gray forest soils].	the decline of methane oxidizing activities in gray forest soil upon its conversion into arable land was shown to be caused by major changes in biotic and physicochemical properties of soil. using the method of immune serums, methane-oxidizing bacteria were detected in both forest and agricultural soils, but their populations differed significantly in both abundance and composition. in the forest soil, the number of methanotrophs was an order of magnitude higher than in arable soil, amounting to ...	2005	15938403
analysis of methane monooxygenase genes in mono lake suggests that increased methane oxidation activity may correlate with a change in methanotroph community structure.	mono lake is an alkaline hypersaline lake that supports high methane oxidation rates. retrieved pmoa sequences showed a broad diversity of aerobic methane oxidizers including the type i methanotrophs methylobacter (the dominant genus), methylomicrobium, and methylothermus, and the type ii methanotroph methylocystis. stratification of mono lake resulted in variation of aerobic methane oxidation rates with depth. methanotroph diversity as determined by analysis of pmoa using new denaturing gradien ...	2005	16204580
[aerobic methanotrophic communities in the bottom sediments of lake baikal].	the results of the first methodical investigation into the aerobic methanotrophic communities inhabiting the bottom sediments of lake baikal are reported. use of the radioisotopic method revealed methane consumption in 12 10- to 50-cm-long sediment cores. the maximum methane consumption rates (495-737 microl/(dm3 day) were recorded in sediments in the regions of hydrothermal vents and oil and gas occurrence. methane consumption was most active in the surface layers of the sediments (0-4 cm); it ...	2005	16211862
impacts of co-solvent flushing on microbial populations capable of degrading trichloroethylene.	with increased application of co-solvent flushing technologies for removal of nonaqueous phase liquids from groundwater aquifers, concern over the effects of the solvent on native microorganisms and their ability to degrade residual contaminant has also arisen. this study assessed the impact of ethanol flushing on the numbers and activity potentials of trichloroethylene (tce)-degrading microbial populations present in aquifer soils taken immediately after and 2 years after ethanol flushing of a ...	2005	15626648
the active methanotrophic community in hydromorphic soils changes in response to changing methane concentration.	methanotrophic communities were studied in several periodically water-saturated gleyic soils. when sampled, each soil had an oxic upper layer and consumed methane from the atmosphere (at 1.75 ppmv). in most gleyic soils the k(m(app)) values for methane were between 70 and 800 ppmv. these are higher than most values observed in dry upland soils, but lower than those measured in wetlands. based on cultivation-independent retrieval of the pmoa-gene and quantification of partial pmoa gene sequences, ...	2006	16423018
pseudolabrys taiwanensis gen. nov., sp. nov., an alphaproteobacterium isolated from soil.	a gram-negative, short rod-shaped micro-organism (cc-bb4(t)) was isolated on nutrient agar from soil from sinshe in taichung county, taiwan. analysis of the 16s rrna gene sequence demonstrated that this isolate was novel, as it showed <92 % similarity to the sequences of species of the genera labrys, beijerinckia and methylocystis. the micro-organism did not utilize methylamine or methanol as a substrate, but was able to use several organic acids. the fatty acid profile was different from those ...	2006	17012581
[comparative characterization of cultured methane-oxidizing bacteria by serological and molecular methods].	three stable methane-oxidizing enrichment cultures, sb26, sb31, and sb31a were analyzed by transmission electron microscopy and by serological and molecular techniques. electron microscopy revealed the presence of both type i and type ii methanotrophs in sb31 and sb31a enrichments; only type ii methanotrophs were found in sb26 enrichment. methylosinus trichosporium was detected in all three enrichments by the application of species-specific antibodies. additionally, methylocystis echinoides was ...	2006	16871808
methylocystis rosea sp. nov., a novel methanotrophic bacterium from arctic wetland soil, svalbard, norway (78 degrees n).	a gram-negative, rod-shaped, non-motile, non-spore-forming, pink-pigmented bacterium, sv97t, was isolated from a wetland soil near ny-alesund, svalbard islands, norway (78 degrees n). on the basis of 16s rrna gene sequence similarity, strain sv97t was shown to belong to the alphaproteobacteria and was highly related to a number of non-characterized methylocystis strains with genbank accession nos aj458507 and aj458502 (100 %) and af177299, aj458510, aj458467, aj458471, aj431384, aj458475, aj4584 ...	2006	16514024
diversity of soluble methane monooxygenase-containing methanotrophs isolated from polluted environments.	methanotrophs were enriched and isolated from polluted environments in canada and germany. enrichments in low copper media were designed to specifically encourage growth of soluble methane monooxygenase (smmo) containing organisms. the 10 isolates were characterized physiologically and genetically with one type i and nine type ii methanotrophs being identified. three key genes: 16s rrna; pmoa and mmox, encoding for the particulate and soluble methane monooxygenases respectively, were cloned from ...	2006	16448499
differential effects of nitrogenous fertilizers on methane-consuming microbes in rice field and forest soils.	the impact of environmental perturbation (e.g., nitrogenous fertilizers) on the dynamics of methane fluxes from soils and wetland systems is poorly understood. results of fertilizer studies are often contradictory, even within similar ecosystems. in the present study the hypothesis of whether these contradictory results may be explained by the composition of the methane-consuming microbial community and hence whether methanotrophic diversity affects methane fluxes was investigated. to this end, ...	2006	16461686
identification of a complete methane monooxygenase operon from soil by combining stable isotope probing and metagenomic analysis.	stable isotope probing (sip) allows the isolation of nucleic acids from targeted metabolically active organisms in environmental samples. in previous studies, dna-sip has been performed with the one-carbon growth substrates methane and methanol to study methylotrophic organisms. the methylotrophs that incorporated the labelled substrate were identified with polymerase chain reaction and sequencing of 16s rrna and 'functional genes' for methanotrophs (mxaf, pmoa, mmox). in this study, a sip exper ...	2006	16817932
characterizing a stable methane-utilizing mixed culture used in the synthesis of a high-quality biopolymer in an open system.	to characterize a methane-utilizing poly-beta-hydroxybutyrate (phb)-producing microbial community.	2006	16882146
methylocystis heyeri sp. nov., a novel type ii methanotrophic bacterium possessing 'signature' fatty acids of type i methanotrophs.	a novel species is proposed for two strains of methanotrophic bacteria (h2(t) and sakb1) isolated from an acidic (ph 4.3) sphagnum peat bog lake (teufelssee, germany) and an acidic (ph 4.2) tropical forest soil (thailand), respectively. cells of strains h2(t) and sakb1 were aerobic, gram-negative, non-motile, straight or curved rods that were covered by large polysaccharide capsules and contained an intracytoplasmic membrane system typical of type ii methanotrophs. they possessed both a particul ...	2007	17329771
identity of active methanotrophs in landfill cover soil as revealed by dna-stable isotope probing.	a considerable amount of methane produced during decomposition of landfill waste can be oxidized in landfill cover soil by methane-oxidizing bacteria (methanotrophs) thus reducing greenhouse gas emissions to the atmosphere. the identity of active methanotrophs in roscommon landfill cover soil, a slightly acidic peat soil, was assessed by dna-stable isotope probing (sip). landfill cover soil slurries were incubated with (13)c-labelled methane and under either nutrient-rich nitrate mineral salt me ...	2007	17714486
identification of active methanotrophs in a landfill cover soil through detection of expression of 16s rrna and functional genes.	active methanotrophs in a landfill soil were revealed by detecting the 16s rrna of methanotrophs and the mrna transcripts of key genes involved in methane oxidation. new 16s rrna primers targeting type i and type ii methanotrophs were designed and optimized for analysis by denaturing gradient gel electrophoresis. direct extraction of rna from soil enabled the analysis of the expression of the functional genes: mmox, pmoa and mxaf, which encode subunits of soluble methane monooxygenase, particula ...	2007	17922768
methylocystis hirsuta sp. nov., a novel methanotroph isolated from a groundwater aquifer.	strain csc1(t), a gram-negative, aerobic, methane-oxidizing bacterium, was isolated from an uncontaminated aquifer nearly 20 years ago. based on 16s rrna gene sequence similarity, this strain was identified as a member of the alphaproteobacteria, most closely related to an uncultured member of the methylocystaceae as well as two cultured organisms, methylocystis sp. l32 and methylocystis sp. sc2. this strain differed from extant species in cell shape, size, expression of soluble methane monooxyg ...	2007	17684277
effect of temperature on composition of the methanotrophic community in rice field and forest soil.	temperature change affects methane consumption in soil. however, there is no information on possible temperature control of methanotrophic bacterial populations. therefore, we studied ch(4) consumption and populations of methanotrophs in an upland forest soil and a rice field soil incubated at different temperatures between 5 and 45 degrees c for up to 40 days. potential methane consumption was measured at 4% ch(4). the temporal progress of ch(4) consumption indicated growth of methanotrophs. bo ...	2007	17725622
diversity of the active methanotrophic community in acidic peatlands as assessed by mrna and sip-plfa analyses.	the active methanotroph community was investigated for the first time in heather (calluna)-covered moorlands and sphagnum/eriophorum-covered uk peatlands. direct extraction of mrna from these soils facilitated detection of expression of methane monooxygenase genes, which revealed that particulate methane monooxygenase and not soluble methane monooxygenase was probably responsible for ch(4) oxidation in situ, because only pmoa transcripts (encoding a subunit of particulate methane monooxygenase) ...	2008	18093158