Publications

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caulobacter versus bacillus spec. div. 195114882305
[the knowledge on hyphomicrobium vulgare stutzer et hartleb]. 195313159234
studies on a strain of caulobacter from water. i. isolation and identification as caulobacter vibrioides henrici and johnson with emended description. 195413183928
studies on a strain of caulobacter from water. ii. nutrition, with implications for cytology. 195413183929
[the presence of caulobacter species in distilled water]. 195413229323
[caulobacter as an unusual contamination of preparations for electron microscopy]. 195414378210
intracellular structures in caulobacter vibrioides. 195413201555
caulobacter; its morphogenesis, taxonomy and parasitism. 195514350601
[nutrition and physiology of caulobacter]. 195613355427
[budding bacteria]. 196114009937
[on w ater myxobacteria]. 196213900960
[the effect of antibiotics on the lysis of chlorella pyrenoidosa pringh cultures]. 196214003319
motile marine bacteria. i. techniques, ecology, and general characteristics.leifson, einar (loyola university, chicago, ill.), b. j. cosenza, r. murchelano, and r. c. cleverdon. motile marine bacteria. i. techniques, ecology, and general characteristics. j. bacteriol. 87:652-666. 1964.-aerobic, heterotrophic bacteria were isolated from the waters of the long island sound, narragansett bay, atlantic ocean, and from the intestine of a variety of marine animals found along the shore of the long island sound. a total of about 600 cultures of motile bacteria were studied mor ...196414129669
[bacteria of the caulobacter genus accompanying algae in culture]. 196414206065
biological properties and classification of the caulobacter group. 196414220656
the fine structure of stalked bacteria belonging to the family caulobacteraceae.the fine structure of a series of stalked bacteria belonging to the genera caulobacter and asticcacaulis has been examined in thin sections. the cell wall has the multilayered structure typical of many gram-negative bacteria, and continues without interruption throughout the length of the stalk. the core of the stalk, continuous with the cytoplasmic region of the cell, is enclosed in an extension of the cell membrane, and contains a system of internal membranes: it is devoid of ribosomes and nuc ...196414245437
the internal membranes of caulobacter crescentus. 19664161779
observations on the adsorption of caulobacter bacteriophages containing ribonucleic acid. 19665969754
the development of cellular stalks in bacteria.extensive stalk elongation in caulobacter and asticcacaulis can be obtained in a defined medium by limiting the concentration of phosphate. caulobacter cells which were initiating stalk formation were labeled with tritiated glucose. after removal of exogenous tritiated material, the cells were subjected to phosphate limitation while stalk elongation occurred. the location of tritiated material in the elongated stalks as detected by radioautographic techniques allowed identification of the site o ...19665960805
intracellular development of a large dna bacteriophage lytic for caulobacter crescentus. 19675602462
[on the distribution of caulobacter in some soils]. 19675618856
[occurrence of caulobacter group bacteria in reservoirs of the volgo-don channel]. 19674238215
[methods of quantitative identification and isolation of caulobacter]. 19684899379
caulobacter crescentus mutants with short stalks.limitation of inorganic phosphate in the culture medium allows stalk elongation in wild-type caulobacter crescentus. mutants unable to form long stalks were isolated.19695784227
[some problems in caulobacter ecology]. 19695371815
[the morphology and development of caulobacter]. 19705493683
properties of caulobacter ribonucleic acid bacteriophage phi cb5.the ribonucleic acid (rna) bacteriophage phicb5, which specifically infects only one form of the dimorphic stalked bacterium caulobacter crescentus, has been obtained in high yield. since the phage is extremely salt-sensitive, a purification procedure was devised which avoided contact with solutions of high ionic strength. phage phicb5 was studied with respect to the physical and chemical properties of both the phage and its rna. in an electron microscope, the phage particles appear as small pol ...19705495512
stalked bacteria: properties of deoxriybonucleic acid bacteriophage phicbk.the caulobacter crescentus bacteriophage phicbk was studied with respect to the physical and chemical properties of both the phage and its deoxyribonucleic acid (dna). electron micrographs reveal the phage to be among the largest dna bacteriophages reported, with head dimensions of 64 by 195 nm and a flexible tail 275 nm in length. the phage is composed of 57% dna. this dna is double-stranded as indicated by (i) the sharp increase in extinction coefficient over a narrow range of temperature incr ...19704193836
specific assay for differentiation in the stalked bacterium caulobacter crescentus.the bacterium caulobacter crescentus carries out a distinct differentiation process during its life cycle. cultures of the bacterium have been synchronized and an assay has been developed for monitoring the course of morphogenesis by the selective adsorption of radioactive rna bacteriophage.197016591854
bacterial differentiation.the foregoing studies are intended to define a differentiation process and to permit genetic access to the mechanisms that control this process. in order to elucidate the basic mechanisms whereby a cell dictates its own defined morphogenic changes, we have found it helpful to study an organism that can be manipulated both biochemically and genetically. we have attempted to develop the studies initiated by poindexter,stove and stanier, and schmidt and stanier (16, 17, 20) with the caulobacter gen ...19715572165
incidence of prosthecate bacteria in a polluted stream.water samples were collected aseptically several times throughout the year at nine stations on the red cedar river, a stream flowing through farmland and receiving effluent from several municipalities in central michigan. total prosthecate bacteria were enumerated by both direct and viable counting techniques. by direct techniques, these bacteria accounted for 0.62 to 1.1% of the total microflora during the study. the predominant type of appendaged bacteria was the caulobacters (caulobacter, ast ...19714943266
[development of caulobacter in mixed cultures]. 19715002168
[physiological properties of bacteria of the genus caulobacter]. 19714256892
large scale isolation of the caulobacter bacteriophage øcb5 and its rna genome. 197111946189
role of transcription in the temporal control of development in caulobacter crescentus (stalk-rifampin-rna synthesis-dna synthesis-motility).the requirement for transcription during development of the stalked bacterium, caulobacter crescentus, was studied with synchronous cultures of swarmer cells. the developmental pattern in these bacteria was first established by determination of the times at which specific changes in cell structure and function occurred. these changes could be divided into those characteristic of (a) development of the swarmer cell into the stalked cell: loss of motility and synthesis of the stalk, and (b) develo ...19724501124
effects of cyclic nucleotides and nucleoside triphosphates on stalk formation in caulobacter crescentus.extensive stalk elongation in skl mutants of caulobacter crescentus occurs when they are grown in complete medium. this stalk elongation is less pronounced in synthetic medium with glucose as the sole carbon source than in complex peptone yeast extract medium. addition of exogenous nucleoside triphosphates (adenosine triphosphate [atp], guanosine triphosphate [gtp], cytidine triphosphate, and uridine triphosphate) inhibits stalk elongation of the skl mutants, whereas cyclic guanosine 3',5'-monop ...19724116756
deoxyribonucleic acid base sequence homologies of some budding and prosthecate bacteria.the genetic relatedness of a number of budding and prosthecate bacteria was determined by deoxyribonucleic acid (dna) homology experiments of the direct binding type. strains of hyphomicrobium sp. isolated from aquatic habitats were found to have relatedness values ranging from 9 to 70% with strain "ea-617," a subculture of the hyphomicrobium isolated by mevius from river water. strains obtained from soil enrichments had lower values with ea-617, ranging from 3 to 5%. very little or no homology ...19725018022
chromosome replication during development in caulobacter crescentus. 19725022192
differential phage sensitivity of cell types in caulobacter. 19725079089
structural studies on the capsid of caulobacter crescentus bacteriophage phicbk. 19724635986
characterization of a protein acyl kinase from caulobacter crescentus. 19724674284
dependence of cell division on the completion of chromosome replication in caulobacter.the relationship between chromosome replication and cell division in the stalked bacterium caulobacter crescentus has been investigated. two compounds, hydroxyurea and mitomycin c, were found to inhibit completely deoxyribonucleic acid (dna) synthesis while allowing continued cell growth and elongation. when these inhibitors were added to exponentially growing cultures, cell division stopped after 38 min when hydroxyurea was used and after 33 min when mitomycin c was used. the period of continue ...19725030621
effect of dibutyryladenosine 3':5'-cyclic monophosphate on growth and differentiation in caulobacter crescentus.caulobacter crescentus goes through a series of morphological changes during its life cycle, including the coincident expression of synthesis of flagella, pili, and receptor sites for dna bacteriophage. upon transfer of a mixed population of cells to medium containing lactose as the sole carbon source, these changes were blocked for about 20 hr until beta-galactosidase activity became apparent. the addition of dibutyryl cyclic amp to the blocked cultures brought about the resumption of cell diff ...19724338585
ultrastructural study of crossbands occurring in the stalks of caulobacter crescentus.an ultrastructural study of crossbands isolated from caulobacter crescentus sk1 418 stalks permitted a view of these structures in a plane other than the perpendicular view normally presented in the electron microscope. these crossbands were composed of concentrically arranged, alternating light and dark bands when stained with phosphotungstic acid. evidence supports the hypothesis that crossbands appear to compose a structural barrier extending the width of the stalk and may function to support ...19734126821
synthesis and structure of caulobacter crescentus flagella.during the normal cell cycle of caulobacter crescentus, flagella are released into the culture fluid as swarmer cells differentiate into stalked cells. the released flagellum is composed of a filament, hook, and rod. the molecular weight of purified flagellin (subunit of flagella filament) is 25,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the formation of a flagellum opposite the stalk has been observed by microscope during the differentiation of a stalked cel ...19734688664
[systematics and classification of the genus caulobacter]. 19734722130
deoxyribonucleic acid-dependent ribonucleic acid polymerase of caulobacter crescentus.deoxyribonucleic acid-dependent ribonucleic acid (rna) polymerase (ec 2.7.7.6) was purified from the dimorphic bacterium caulobacter crescentus at three stages in development. enzyme from pure populations of stalked cells, as well as populations enriched in swarmer and predivisional cells, appeared identical in subunit structure and template requirements. the molecular weights of the enzyme subunits were 165,000, 155,000, 101,000, and 44,000, respectively. by analogy with rna polymerase from oth ...19734734061
caulobacter crescentus bacteriophage phicbk: structure and in vitro self-assembly of the tail. 19734767460
the growth and partition of cell membranes during synchronized division cycle of caulobacter crescentus. 19734778922
the mechanism of a host-dependent abortive bacteriophage infection in caulobacter. 19734349467
messenger selection by bacterial ribosomes.the counterpart of escherichia coli initiation factor 3(if-3) was isolated from caulobacter crescentus, purified to homogeneity, and used in comparative studies on in vitro translation of rna from the c. crescentus rna phage cb5 and of coliphage ms2 rna. the two phage rnas are similar in physical properties and analogous in genetic content. the factor, c-if-3, substitutes for e. coli if-3 and promotes correct translation of ms2 rna by e. coli ribosomes. conversely, e. coli if-3 substitutes for c ...19734525171
crossbands of caulobacter crescentus stalks serve as indicators of cell age. 19734586107
deoxyribonucleic acid methylation and development in caulobacter bacteroides.the stalked bacterium caulobacter bacteroides, which displays a series of developmental changes during its life cycle, was shown to contain the methylated deoxyribonucleic acid (dna) nucleotide bases n(6)-methyladenine and 5-methylcytosine as well as the enzymes required for the synthesis of these bases. a difference in the specific activity of these enzymes was observed between swarmer cells, which are low in activity, and stalked cells, which are high in activity. this difference was not refle ...19734745424
purification and properties of initiation factor if-3 from caulobacter crescentus. 19744594127
polypeptide chain initiation in caulobacter crescentus without initiation factor if-1. 19744594128
a pleiotropic mutation affecting expression of polar development events in caulobacter crescentus.a developmental mutant of c. crescentus with altered polar surface structures has been isolated. the mutant lacks a flagellum and pili, and may have an abnormal dna phage receptor site. a revertant regains the normal structures simultaneously. this point mutation allows normal flagellin synthesis, stalk formation, equatorial cell division, and rate of growth. the mutant phenotype indicates that the assembly of the polar surface structures is coordinately regulated and independent of mechanisms r ...19744212892
chromosomes segregration and development in caulobacter crescentus. 19744217842
bacteriophage structure: determination of head-tail symmetry mismatch for caulobacter crescentus phage phicbk.electron micrographs of negatively stained bacteriophage [unknown]cbk have been analyzed by fourier methods. computer-calculated fourier transforms that contain phase as well as magnitude information have established fivefold rotational symmetry for the head and threefold rotational symmetry for the tail. these results indicate that a symmetry match is not necessarily required between separate structural components of a bacteriophage.19744132371
glucose-6-phosphate dehydrogenase from caulobacter crescentus. 19744152892
purification and characterization of guanylate cyclase from caulobacter crescentus. 19744155295
a flagella specific bacteriophage for caulobacter. 19744820673
structure and protein distribution for the capsid of caulobacter crescentus bacteriophage phicbk. 19744854434
interaction of escherichia coli 30s ribosomal subunits with ms2 phage rna in the absence of initiation factors.ms2 rna binds at 0 degrees to 30s subunits from e. coli and, to a smaller extent, to those of a pseudomonas species, as judged by filtration on nitrocellulose membranes; this mrna does not bind to 30s subunits from bacillus brevis or caulobacter crescentus. binding does not depend on the presence of initiation factors; it is sensitive to aurintricarboxylic acid but insensitive to edeine and is competitive with such synthetic polynucleotides as poly(u) and poly(aug). complex formation can also be ...19744610580
stalk formation and its inhibition in caulobacter crescentus.estimates of average rates of stalk formation over several generations of growth in caulobacter crescentus showed that long-stalked sk1 mutant and phosphate-starved wild-type cultures produce stalk material at about twice the rate of wild-type c. crescentus grown with adequate nutrients. thus, the long stalks of sk1 or phosphate-starved caulobacters are not merely a function of their longer doubling times. inhibition of cell division of sk1 418 with mitomycin c (mc) caused production of cellular ...19744436259
pattern of unequal cell division and development in caulobacter crescentus. 1975805732
gene transfer in caulobacter crescentus: polarized inheritance of genetic markers.recombination frequencies were determined for 15 independently isolated auxotrophs of c. crescentus crossed pairwise in all possible combinations. the results indicate that the mutants may be grouped into at least two types: "fertile" strains, which recombine with all other mutants at frequencies ranging from less than 10-6 to 3 times 10-2, and "nonfertile" strains which recombine with fertile strains at high frequencies and with other nonfertile strains at low or negligible frequencies. several ...19751137965
purification of cell wall peptidoglycan of the dimorphic bacterium caulobacter crescentus. 19751164030
a specific cyclic guanosine 3':5'-monophosphate-binding protein in caulobacter crescentus.a binding protein specific for cyclic guanosine 3':5'-monophosphate (cyclic gmp) has been partially purified from extracts of the eubacterium caulobacter crescentus and resolved from cyclic adenosine 3':5'-monophosphate (cyclic amp)-binding activity. binding of cyclic gmp is not affected by the addition of cyclic amp or 5'-gmp, but is inhibited about 50 percent by a 50-fold molar excess of dibutyryl cyclic gmp or cyclic hypoxanthine 3':5'-monophosphate. the apparent dissociation constant for the ...1975168209
regulation of the caulobacter cell cycle. 1975164329
terminal-sequence analysis of bacterial ribosomal rna. correlation between the 3'-terminal-polypyrimidine sequence of 16-s rna and translational specificity of the ribosome.the 3'-terminal sequences of 16-s ribosomal rna from a number of bacteria have been determined by a stepwise degradation and 3'-terminal labelling procedure. the sequences obtained were: bacillus stearothermophilus, -g(z)approximately 5 y-u-c-c-u-u-u-c-u (a); b. subtilis, -g(z)approximately 7 y-c-u-u-u-c-u; caulobacter crescentus, -g(z)3 y-u-c-c-u-u-u-c-u; pseudomonas aerugionosa, -g-z-z-y-c-u-c-u-c-c-u-u(a), where z is any nucleotide other than g. thus, as previously found in escherichia coli, ...1975809282
ribosomal protein s1 and polypeptide chain initiation in bacteria.among several subspecies of 30s subunits of escherichia coli observed by polyacrylamide-agarose gel electrophoresis, only the slow-moving, protein s1-containing subspecies participates in the formation of the 30s initiation complex with coliphage ms2 rna as mrna; the other subspecies retain activity with aug as mrna; they are also active in the poly(u)-directed binding of phe-trna. protein s1 from caulobacter crescentus substitutes for e. coli s1 despite the fact that c. crescentus ribosomes do ...19751094462
poly(adenylic acid) sequences in the rna of caulobacter crescenus.poly(adenylic acid) sequences have been isolated from the gram-negative bacterium caulobacter crescentus. most of these a-rich tracts are associated with large rna molecules that constitute an important fraction of the unstable rna in these bacteria, and, as estimated by poly(u) filter binding, they are not present in the 16s or 23s ribosomal rna. preliminary estimates of size from polyacrylamide gel electrophoresis suggest that the majority of the a-rich tracts ranges from 15 to approximately 5 ...19751094464
polarity of gene transfer in caulobacter. 19751202158
synchronous cell differentiation in caulobacter crescentus.the growth of a stalked bacterium, caulobacter crescentus, has been synchronized easily and reproducibly by a new method. when this bacterium is grown to a late log phase in nutrient broth at 30 c with aeration, swarmer cells are accumulated in the culture to 80% of the whole cell population. when this culture is inoculated into fresh pre-warmed broth at twentyfold dilution, it immediately initiates synchronous cell growth. simultaneously, synchronous cell differentiation is monitored by the sus ...19751230510
effect of cis-platinum(ii)diamminodichloride on cell division of hyphomicrobium and caulobacter.low concentrations of the radiomimetic agent cis-platinum(ii)diamminodichloride (pdd) inhibited cell division in caulobacter crescentus (0.1 mug/ml) and hyphomicrobium sp. strain b-522 (1.0 mug/ml) without altering the length of prosthecae. after exposure, cells of c. crescentus appeared as long filaments, whereas only the bud portion of hyphomicrobium underwent elongation. pdd-treated cells of both species were multinucleated. after the removal of pdd by washing, filaments of c. crescentus frag ...19761245459
isolation and characterization of caulobacter crecentus bacteriophage phi cd1.a dna-containing bacteriophage, phicd1, was isolated from sewage and shown to infect both stalked and swarmer cells of caulobacter crescentus strain cb13b1a. phicd1 is a small, icosohedral bacteriophage, 60 nm in diameter, which possesses a short, noncontractile tail, 10 to 12 nm in length. the bacteriophage particle is composed of at least eight structural proteins. phicd1 nucleic acid exists as a linear duplex of dna as judged by: (i) thermal denaturation (tm), (ii) cscl density gradient centr ...19761255848
prosthecobacter fusiformis nov. gen. et sp., the fusiform caulobacter.four strains of heterotrophic, fusiform caulobacters have been isolated from freshwater sources. a single prostheca extends from one pole of mature cells, and cells attach to various substrata by means of a holdfast located at the distal tip of the appendage. thus, superficially these bacteria bear a strong resemblance to bacteria in the genus caulobacter. however, unlike caulobacter these bacteria do not exhibit a dimorphic life cycle of motile, non-stalked daughter cells and immotile, stalked ...19761086646
structure of caulobacter deoxyribonucleic acid.the deoxyribonucleic acid of the dimorphic bacterium caulobacter crescentus contains a component that renatures with rapid, unimolecular kinetics. this component was present in both swarmer and stalked cells and exhibited the sensitivity to endonuclease s1 expected for hairpin loops. double-stranded side branches between 100 and 600 nucleotide pairs in length were visible in electron micrographs of rapidly reassociating deoxyribonucleic acid isolated by hydroxyapatite chromatography. no extrachr ...1976947891
fatty acid composition of selected prosthecate bacteria.the cellular fatty acid composition of 14 strains of caulobacter speices and types, two species of prosthecomicrobium, and two species of asticcacaulis was determined by gas-liquid chromatography. in most of these bacteria, the major fatty acids were octadecenoic acid (c18:1), hexadecenoic acid (c16:1) and hexadecanoic acid (c16:0). some cyclopropane and branched chain fatty acids were detected in addition to the straight chained acids. hydroxytetradecanoic acid was an important component of p.e ...19761015941
amino acid composition of peptidoglycan in caulobacter crescentus.peptidoglycan of a gram-negative stalked bacterium, caulobacter crescentus cb13, contained alanine, diaminopimelic acid, and glutamic acid, in molar ratios of 2 : 1 : 1. the amino acid compositions of peptidoglycans isolated from cultures enriched in swarmer and stalked cells, and from a stalk-less mutant were similar. this finding conflicts with a previous observation that swarmer peptidoglycan does not contain diaminopimelic acid (goodwin and shedlarski (1975) arch. biochem. biophys. 170, 23-3 ...19761018023
physical characterization of caulobacter crescentus flagella.preparations of intact flagella isolated from caulobacter crescentus cb13b1a were found to contain two protein species of apparent molecular weights 28,000 and 25,000. both proteins cross-reacted completely with each other and with purified flagella in ouchterlony double-immunodiffusion assays. the amino acid compositions of the isolated proteins were similar to one another but precluded any precursor-product relationship. absence of both the 25,000- and 28,000-molecular-weight proteins from a n ...1976824276
pathway of glucose catabolism in caulobacter crescentus.glucose when present as a sole organic carbon source in a mineral salts medium is dissimilated by caulobacter crescentus atcc 15252 (strain cb-2) by the entner-doudoroff pathway throughout the culture cycle (exponential, transition, and stationary phase). most of the available glucose that is present at the onset of exponential growth is assimilated by the cells during the transition phase or the period associated with stalk cell development. swarmer cell development is minimized during this pha ...197618652
differentiation in the caulobacter cell cycle. 1976185940
effect of 3':5'-cyclic gmp derivatives on the formation of caulobacter surface structures.exogenous derivatives of 3':5'-cyclic gmp, 8-bromo- and n2,o2'-dibutyryl cyclic gmp, coordinately repress surface structure differentiation in caulobacter crescentus. growth in the presence of cyclic gmp derivatives resulted in the loss of flagella and pili formation and concomitant resistance to both dna phage phicbk and rna phage phicb5 infection without affecting growth rate, stalk formation, and equatorial cell division. the effect of cyclic gmp derivatives was shown to be the repression of ...1976184471
a flagellotropic bacteriophage and flagella formation in caulobacter. 1976936475
isolation and characterization of rna phages for caulobacter crescentus. 1976960574
requirement for cellular associations in the development of caulobacter crescentus. 1976962922
requirement of a cell division step for stalk formation in caulobacter crescentus.penicillin g at low concentrations blocked cell division in caulobacter crescentus without inhibiting cell growth. the long filamentous cells formed after two to three generations under these conditions had a stalk at one pole and usually one or more flagella at the opposite pole. the failure of the filaments to form a second stalk at the flagellated pole indicates that stalk formation was dependent upon completion of a step that was also required for cell division. two observations support this ...1976977541
conditional surface structure mutants of caulobacter crescentus temperature-sensitive flagella formation due to an altered flagellin monomer. 19761003462
regulation of polar surface structures in caulobacter crescentus: pleiotropic mutations affect the coordinate morphogenesis of flagella, pili and phage receptors.a large number of caulobacter mutants resistant to dna or rna phages were isolated. these phage-resistant mutants exhibited phenotypic variations with respect to cell motility and sensitivity to other phages. the majority of the mutants was resistant to both dna and rna phages tested. in addition, these mutants were either motile or non-motile. the analysis of spontaneous revertants from these mutants indicated that a single mutation is involved in these phenotypic variations. other mutants were ...19761012268
adsorption properties of stage-specific caulobacter phage phicbk. 1977841867
isolation of spontaneously derived mutants of caulobacter crescentus.caulobacter crescentus has a penicillinase which precludes the use of penicillin for mutant enrichment. however, two other antibiotics, fosfomycin and d-cycloserine, can be enrich for c. crescentus mutants. in enrichment procedures for c. crescentus auxotrophs, spontaneously derived mutants occur at a frequency of 5-10% among the survivors of an enrichment procedure. consequently, large numbers of mutants are readily obtained without any need for mutagenesis. these mutants are heterogeneous both ...1977407126
caulobacter flagellins.flagellins from 17 caulobacter strains were partially purified. their molecular weights and immunological properties indicated that a two-component flagellin system is common among caulobacters.1977914783
transfer and expression of pseudomonas plasmid rp1 in caulobacter.this study demonstrates that the host range of pseudomonas plasmid rp1 includes the genus caulobacter. caulobacter was shown to acquire three antibiotic resistance markers located in rp1. a fourth plasmid marker, susceptibility to an rna bacteriophage, was not expressed, but could be transferred from caulobacter to escherichia coli. the lack of phenotypic expression of the phage marker was manifested by the inability of the phage to adsorb or to produce plaques on caulobacter transcipients. mati ...1977406354
patterns of protein synthesis during development in caulobacter crescentus. 1977849807
[species composition of heterotrophic bacteria in the water of the rybinek reservoir].twenty four cultures of heterotrophic bacteria were isolated from the water of the rybinsk reservoir on a medium containing organic matter. bacteria belonging to the families pseudomonadaceae and achromobacteriaceae were most widely distributed; bacteria of the mycobacteriaceae family were encountered less often. predominating species which belonged to the genera pseudomonas, caulobacter, flavobacterium, mycobacterium, and corynebacterium were isolated from the central part of the reservoir. the ...1977142898
effect of carbon source and the role of cyclic adenosine 3',5'-monophosphate on the caulobacter cell cycle.the expression of cell cycle events in caulobacter crescentus cb13 has been shown to be associated with regulation of carbohydrate utilization. growth on lactose and galactose depends on induction of specific enzymes. prior growth on glucose results in a delay in enzyme expression and cell cycle arrest at the nonmotile, predivisional stage. dibutyryl cyclic adenosine 3',5'-monophosphate (amp) was shown to stimulate expression of the inducible enzymes and, thus, the initiation of the cell cycle. ...1977197060
host factor for coliphage q beta rna replication: presence in procaryotes and association with the 30s ribosomal subunit in escherichia coli.the host factor required for in vitro coliphage q beta rna replication, a heat-stable rna binding protein present in uninfected escherichia coli, has been detected by both immunological and functional tests in acinetobacter calcoaceticus, klebsiella pneumoniae, pseudomonas aeruginosa and pseudomonas putida. it was not detectable by these criteria in bacillus stearothermophilus, bacillus subtilis, caulobacter crescentus, micrococcus lysodeikticus, rhodopseudomonas capsulata or saccharomyces cerev ...1977329101
effect of macromolecular synthesis on the coordinate morphogenesis of polar surface structures in caulobacter crescentus.the caulobacter polar surface structures (flagella, pili, and the deoxyribonucleic acid phage phicbk receptors), which are expressed at proximal sites of swarmer cells in a coordinate manner (shapiro, annu. rev. microbiol., 30:377-407, 1976) could be blocked by a single mutation. the mutant c. crescentus cb13 ple-801 did not form these surface structures when grown at 35 degrees c. upon shift down to 25 degrees c, the mutant cells initiated the formation of the surface structures. when mitomycin ...1977405372
reconstitution and purification of flagellar filaments from caulobacter crescentus.filaments from isolated flagella of caulobacter crescentus have been purified by successive dissociation and reconstitution. after the second and third reconstitutions from subunits in 0.8 m sodium citrate, filament preparations contained only two proteins, flagellin a (26,000 daltons) and flagellin b (28,000 daltons). there was some enrichment for flagellin a during reconstitution by this procedure, since isolated flagella contained flagellin a and flagellin b in a ratio of approximately 3.8:1 ...1977336599
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