Publications

dugbe virus: a new tick-borne arbovirus from nigeria.		1973	4785446
propagation and plaquing of dugbe virus (an ungrouped nigerian arbovirus) in various mammalian and arthropod cell lines.		1974	4212275
dugbe virus: a tick-borne arbovirus from nigeria.		1974	4365798
letter: isolation of dugbe virus from amblyoma variegatum ticks in cameroun.		1974	4458154
effect of route of inoculation on histopathological changes induced in mice by dugbe virus (a nigerian tick-borne virus).		1974	4479820
cytological, cytochemical and immuno-fluorescence studies with dugbe virus - a new nigerian tick-borne virus.	dugbe virus, a new tick-borne arbovirus from nigeria, was propagated in continuous porcine kidney (ps) cells, and the cytopathology studied by various staining techniques such as may-grunwald-giemsa, methyl-green pyronine, feulgen, and immuno-fluorescence. the gross cytopathology was slight and infected cells continued to undergo normal mitotic divisions. the outstanding cytopathologic feature was the presence of large basophilic intracytoplasmic inclusion bodies, which were pyroninophilic and f ...	1975	46127
viruses other than arenaviruses from west african wild mammals. factors affecting transmission to man and domestic animals.	at least thirty-seven different viruses have been isolated from wild mammals in west africa since 1962. some of these, including lassa virus, are already known to cause serious human morbidity and mortality. crimean haemorrhagic fever-congo virus, dugbe virus, mokola virus, and a smallpox-like agent from a gerbil in dahomey are briefly discussed. an account of social and ecologic factors affecting man, domestic animals, and their interaction with wild mammals is given.	1975	1085217
a serological survey of dugbe virus antibodies in nigerians.		1975	1179469
antibodies to dugbe virus in mammalian sera from egypt.		1976	1025257
[surgey of tick-borne arboviruses in the central african republic (1973-1974). isolation of dugbe, chf/congo, jos and bhanja viruses].	as a complement of the study of the epidemiology of the arboviruses in central africa a survey of tick borne viruses has been done, in the central african republic, from december 1972 to june 1974; 15.103 ticks were collected, mainly from cattle at the slaughterhouses. they include: 10.085 ambylomma variegatum, 4.113 boophilus, 542 rhipicephalus and 363 hyalomma. from 892 monospecific pools inoculated into baby mice, 98 isolates of virus were obtained. among the 57 isolates which have been fully ...	1976	1036474
crimean-congo hemorrhagic fever, thogoto, dugbe, and jos viruses isolated from ixodid ticks in ethiopia.	from 1974 through 1976, u.s. naval medical research unit no. 5 isolated 25 strain of tick-borne virus in infant mice from 410 pools containing over 6,000 ticks, and one strain from a bird and one strain from a rodent collected in central and southern ethiopia. of these, 17 were identified as known viruses previously found in west central and east africa. there were 8 strains of jos virus from amblyomma ticks; 7 strains of dugbe virus from a bird, a rodent and from ticks; 1 strains of crimean-con ...	1978	98066
[transovarian passage of dugbe virus in the tick amblyomma variegatum (fabricius)].		1978	719844
the multiplication of dugbe virus in the ixodid tick, hyalomma rufipes koch, after experimental infection.	nymphs and adults of h. rufipes were inoculated intracoelomically with a dugbe virus concentration containing 1.5 ld50/0.02 ml. the virus multiplied to a peak titre of 9.3 log10 +/- 0.03 in nymphs and 4.4 log10 +/- 0.02 in the unfed adults. virus infection persisted during metamorphosis and was passed on to the adults. a blood meal significantly increased the titre of virus in the adults at a probability less than 0.001. also the tire in the engorged females was significantly higher than that in ...	1979	538818
polypeptide synthesis of dugbe virus, a member of the nairovirus genus of the bunyaviridae.	the replication of dugbe (dug) virus, a member of the nairovirus genus of the bunyaviridae, has been investigated. during the infection of bs-c-1 cells a virus-specific c.p.e. was initially observed followed by recovery of the cell monolayer but with continued production of infectious virus. six dug virus-induced polypeptides were identified with apparent molecular weights, determined by gel electrophoresis, of 92000 (p92), 82000 (p82), 77000 (p77), 52000 (p52), 48000 (p48) and 34000 (p34). the ...	1985	3968534
[ecology of tick-borne arboviruses in the central african republic].	ticks have been collected on cattle skins for seven consecutive years in bangui, berberati, bangassou and bouar. 150 strains of arboviruses have been isolated from the four following species: amblyomma variegatum: dugbe, jos, thogoto, crimean-congo hemorragic fever (cchf), yellow fever, bhanja and pétévo; boophilus annulatus: dugbe, jos and thogoto; b. decoloratus: dugbe, jos, thogoto and cchf; hyalomma nitidum: cchf. dugbe (112 isolated strains), jos (19 isolates) and thogoto (11 isolates) viru ...	1985	4028317
persistent infection of aedes albopictus c6/36 cells by bunyamwera virus.	two cell lines persistently infected with bunyamwera virus have been established from the c6/36 clone of aedes albopictus cells. the cells express bunyamwera virus antigens as detected by immunofluorescence and are resistant to superinfection with bunyamwera virus and other bunyaviruses, but not dugbe virus (nairovirus) nor vesicular stomatitis virus. the virus released from the persistently infected cells developed an altered cloudy or "bull's-eye" plaque morphology with increasing passage leve ...	1986	3952984
experimental infection of six species of ixodid ticks with dugbe virus (family bunyaviridae, genus nairovirus).	the vector potential of each of 6 species of colonized north american and african ixodid ticks was assessed by intracoelomic inoculation with dugbe virus (ibar 1792, 14th passage in suckling mouse brain) and viral titers were monitored after selected incubation periods. persistence of dugbe virus for greater than or equal to 53 days in 5 species (dermacentor andersoni, d. variabilis, amblyomma americanum, rhipicephalus appendiculatus, and r. sanguineus) indicates that infection occurred. viral t ...	1989	2496609
difference in vector competence of two species of sympatric ticks, amblyomma variegatum and rhipicephalus appendiculatus, for dugbe virus (nairovirus, bunyaviridae).	amblyomma variegatum was shown to be a competent vector of dugbe (dug) virus whereas rhipicephalus appendiculatus was not. when dug virus was taken up orally by a. variegatum nymphs, during capillary feeding, the virus replicated and persisted through moulting to the following adult stage. in contrast, although dug virus replicated in capillary fed r. appendiculatus nymphs, the virus did not persist trans-stadially into the adult stage. if the oral route of infection was by-passed by direct inoc ...	1989	2510418
dugbe virus replication in nymph and adult amblyomma variegatum.		1989	2772415
studies on the pathogenicity of a nairovirus, dugbe virus, in normal and immunosuppressed mice.	susceptibility to lethal infection with the kt281/75 strain of the tick-borne nairovirus, dugbe virus, was similar in an outbred strain and several inbred strains of mice. for the outbred strain, both neural and extraneural routes of virus inoculation resulted in lethal infection, but susceptibility decreased with age and only intracerebral inoculation produced a lethal infection in adults. in newborn mice, subcutaneous (s.c.) inoculation of virus (analogous to a tick-bite) produced a disseminat ...	1990	2106572
coding strategy of the s rna segment of dugbe virus (nairovirus; bunyaviridae).	the s rna segment of dugbe (dug) virus (nairovirus; bunyaviridae) was sequenced from three overlapping cdna clones and by primer extension. the s rna is 1712 nucleotides in length and contains one large open reading frame (orf) of 1326 nucleotides coding for a 49.4-kda protein on viral complementary (vc) rna. this protein in size corresponds to the dug nucleocapsid (n) protein (p. cash, 1985, j. gen. virol. 66, 141-148). the 49.4-kda product was expressed as a fusion protein with beta-galactosid ...	1990	2327076
structure and morphogenesis of dugbe virus (bunyaviridae, nairovirus) studied by immunogold electron microscopy of ultrathin cryosections.	we have studied the structure and morphogenesis of dugbe (dug) virus (bunyaviridae, nairovirus) in cultured porcine kidney (ps) cells and a tick cell line (ra 243) using immunogold electron microscopy. dug virus is a tickborne arbovirus, considered to be a low health hazard, that is antigenically and genetically related to crimean congo haemorrhagic fever (cchf) virus (marriott et al., 1990). we have investigated the maturation and intracellular transport of dug virus particles as a model for ot ...	1991	1767584
dissemination, replication, and trans-stadial persistence of dugbe virus (nairovirus, bunyaviridae) in the tick vector amblyomma variegatum.	the dissemination and replication of dugbe (dug) virus and its tissue tropisms in the tick vector amblyomma variegatum were examined by immunohistochemical analysis using specific antibody, in situ hybridization with a viral-complementary riboprobe, and infectivity assays of dissected tissues. dugbe virus was localized in both unfed and feeding adults inoculated as nymphs or orally infected by capillary feeding, and in nymphs infected by capillary feeding. in non-feeding ticks, the main sites of ...	1991	1867347
dugbe nairovirus m rna: nucleotide sequence and coding strategy.	the coding assignments of the medium-sized (m) rna segment of the dugbe (dug) virus (nairovirus, bunyaviridae) were investigated. the complete nucleotide sequence of 4888 nucleotides (nt) contained one long open reading frame in the viral complementary rna, extending from an aug start codon at nt 48-50 to a stop codon at nt 4701-4703 (numbered from the 5' terminus of vcrna). comparison of the terminal sequences with the ends of the dug s segment revealed sequence identity between the first nine ...	1992	1387749
expression of the nucleocapsid protein of dugbe virus and antigenic cross-reactions with other nairoviruses.	the small (s) rna segment of dugbe (dug) virus (nairovirus, bunyaviridae) encodes a single protein, the nucleocapsid (n) protein, of m(r) 49.4 kda. cdna derived from the complete coding region for the n protein was cloned into autographa californica nuclear polyhedrosis virus (acnpv) under control of the polyhedrin promoter and used to infect spodoptera frugiperda insect cells. western blotting analysis using monoclonal antibodies demonstrated the production of dug n protein in the infected cell ...	1992	1529645
mechanisms of neutralization of a nairovirus (dugbe virus) by polyclonal igg and igm.	dugbe virus is a member of the nairovirus genus of the bunyaviridae. purified polyclonal anti-dugbe virus igg, which neutralized greater than 99.5% of virus, reduced attachment of virus to bsc-1 cell monolayers by only 36%. a 100-fold lower concentration neutralized virus by 88%, and had no effect upon attachment. neutralizing igg did not affect the ability of dugbe virus to be internalized by or to fuse with bsc-1 cells. this suggests that igg neutralization occurs largely at a stage subsequent ...	1992	1645139
non-viral sequences at the 5' ends of dugbe nairovirus s mrnas.	analyses of the mrna transcription processes of viruses in four genera (bunyavirus, hantavirus, phlebovirus and tospovirus) of the family bunyaviridae have revealed a common mechanism of initiation using host-derived primers, known as cap-snatching. to provide similar information on the fifth genus in the family, the 5' ends of dugbe nairovirus s mrna species were specifically cloned and sequenced. this revealed the presence of non-viral heterogeneous sequences, five to 16 nucleotides in length ...	1993	8409954
investigation of tick-borne viruses as pathogens of humans in south africa and evidence of dugbe virus infection in a patient with prolonged thrombocytopenia.	in the course of investigating suspected cases of viral haemorrhagic fever in south africa patients were encountered who had been bitten by ticks, but who lacked evidence of infection with crimean-congo haemorrhagic fever (cchf) virus or non-viral tick-borne agents. cattle sera were tested by enzyme-linked immunoassay to determine whether tick-borne viruses other than cchf occur in the country. the prevalence of antibody in cattle sera was 905/2116 (42.8%) for cchf virus, 70/1358 (5.2%) for dugb ...	1996	8666081
large rna segment of dugbe nairovirus encodes the putative rna polymerase.	the nucleotide sequence of the large (l) rna segment of dugbe (dug) virus (nairovirus, bunyaviridae) was determined, completing the first entire genome sequence of a nairovirus. the l segment comprised 12255 nucleotides, making a total genome size of 18855 nucleotides, and the ends showed identity with the ends of the medium (m) and small (s) genomic segments. a single open reading frame (orf) was present in the viral complementary strand, sufficient to encode a protein of 459 kda. the predicted ...	1996	8760425
[arbovirus circulation in the republic of guinea].	in 1978-1991 the ussr-guinea virological and microbiological laboratory functioned in kindia, the republic of guinea. arbovirus activity in this country was studied by a number of virologists and other specialists. their personal contribution and achievements in this collaboration are reflected in the present paper. about 74,000 mosquitoes, 100,000 ixodidae ticks, 1,500 wild birds, 2,700 bats, 106 monkeys, and 308 other mammals, 927 blood samples collected from febrile patients were examined in ...	1996	8926934
[sequencing and comparative analysis of the complete glycoprotein gene of three crimean-congo hemorrhagic fever virus chinese isolates].	to determine the complete sequences of glycoprotein (m) gene of crimean-congo hemorrhagic fever virus (cchfv) chinese isolates (xinjiang hemorrhagic fever virus, xhfv) ba66019, ba8402 ba88166 and to compare and analyze the relationships among the viruses.	2001	11436635
dugbe nairovirus s segment: correction of published sequence and comparison of five isolates.	the sequence of the s (small) rna segment of the ard 44313 isolate of dugbe nairovirus (dug) has been redetermined, and a number of apparent errors in the previously reported sequence (v. k. ward, a. c. marriott, a. a. el-ghorr, and p. a. nuttall, 1990, virology 175, 518-524) were revealed. our results indicate that the s rna is 1716 nucleotides (nt) in length and contains one large open reading frame spanning 1449 nt. this can encode a 483 amino acid polypeptide, m(r) 53.9 kda, corresponding to ...	2002	12009878
nairobi sheep disease virus, an important tick-borne pathogen of sheep and goats in africa, is also present in asia.	nairobi sheep disease (nsd) virus is the prototype of the tick-borne nsd serogroup, genus nairovirus, family bunyaviridae. it is highly pathogenic for sheep and goats, causes disease in humans, and is widespread throughout east africa. ganjam virus has caused disease in goats and humans in india. due to their occurrence on different continents and association with different ticks, these viruses were considered distinct despite serologic cross-reactivity. their s rna genome segments and encoded n ...	2002	12482666
inhibition of dugbe nairovirus replication by human mxa protein.	sensitivity to the interferon-induced protein, mxa, has previously been demonstrated for viruses belonging to the orthobunyavirus, hantavirus and phlebovirus genera of the bunyaviridae family. we have extended these findings to a member of the fourth and remaining genus containing viruses that infect man and other animals, the nairovirus dugbe virus (dugv). indirect immunofluorescence experiments using va9 cells (vero cells permanently transfected with mxa cdna) revealed strongly reduced dugv an ...	2004	14687945
crimean-congo hemorrhagic fever virus genome l rna segment and encoded protein.	sequence analysis of the l rna genome segment and predicted encoded l polymerase protein of crimean-congo hemorrhagic fever (cchf) virus (genus nairovirus, family bunyaviridae) demonstrates that they are approximately twice the size of those found in viruses of other bunyavirus genera. the cchf virus l segment and encoded protein (12,164 nucleotides and 3944 amino acids, respectively) are similar in size and sequence to those of the nairovirus dugbe virus (12,255/62% and 4036/62% nucleotide and ...	2004	15033562
[the mammals of guinea as reservoirs and carriers of arboviruses].	a great body of data on the fauna and ecology of wild mammals and their participation in the circulation of arboviruses were collected when the ecology of the latter of the republic of guinea was studied in 1978 to 1989. a hundred and eighteen species belonging to 10 orders were identified. over 2,000 biological specimens were virologically and serologically examined. six arboviruses were isolated. these included dugbe virus (from the hussar monkey cercopithecus (erythrocebus patas) and 5 viruse ...	2006	16562748
pathogenesis of dugbe virus infection in wild-type and interferon-deficient mice.	in 129 mice, infection with the nairovirus dugbe virus (dugv) was lethal following intracerebral but not intraperitoneal inoculation. following both routes of inoculation, immunostaining of tissue sections demonstrated virus-positive cells in the brain, indicating that dugv is neuroinvasive in mice. many brain areas were affected and neurones were the main cell type infected. infected cells showed punctate accumulations of viral nucleoprotein in the cytoplasm, indicative of virus replication sit ...	2006	16760403
tickborne arbovirus surveillance in market livestock, nairobi, kenya.	to identify tickborne viruses circulating in kenya and the surrounding region, we conducted surveillance at abattoirs in nairobi, kenya. species of ticks collected included rhipicephalus pulchellus (56%), amblyomma gemma (14%), r. appendiculatus (8%), a. variegatum (6%), and others. a total of 56 virus isolates were obtained, 26 from a. gemma, 17 from r. pulchellus, 6 from a. variegatum, and 7 from other species. virus isolates included dugbe virus (dugv), an unknown virus related to dugv, thogo ...	2006	16836823
kupe virus, a new virus in the family bunyaviridae, genus nairovirus, kenya.	we have previously described isolation and preliminary identification of a virus related to dugbe virus (dugv), family bunyaviridae, genus nairovirus. six isolates of the virus were obtained from pools of amblyomma gemma and rhipicephalus pulchellus ticks collected from hides of cattle in nairobi, kenya, in october 1999. we report results of further characterization of this virus, including growth kinetics in cell culture and full-length genome sequencing and genetic characterization, which show ...	2009	19193256
differential activation profiles of crimean-congo hemorrhagic fever virus- and dugbe virus-infected antigen-presenting cells.	crimean-congo hemorrhagic fever virus (cchfv) is a highly pathogenic, tick-borne member of the family bunyaviridae and the genus nairovirus. to better elucidate the pathogenesis of cchfv, we analysed the host innate immune response induced in antigen-presenting cells (apcs) infected in vitro by cchfv. monocyte-derived dendritic cells (dcs) and macrophages (mps) were both shown to be permissive for cchfv and to replicate the virus, as monitored by genomic and antigenomic strand quantification. vi ...	2010	19812268
development of a one step real time rt-pcr assay to detect and quantify dugbe virus.	a one-step real time quantitative rt-pcr (qrt-pcr) assay was developed to detect all published dugbe virus (dugv) genomes of the nairovirus genus. primers and probes were designed to detect specific sequences on the most conserved regions of the s segment. the limit of detection of the assay was 10 copies per reaction which is an improvement of 3 log(10)ffu/ml over the sensitivity of conventional rt-pcr. the specificity of the primers and probe was confirmed with the closely related nairoviruses ...	2011	21703306
inhibition of interferon induction and action by the nairovirus nairobi sheep disease virus/ganjam virus.	the nairoviruses are an important group of tick-borne viruses that includes pathogens of man (crimean congo hemorrhagic fever virus) and livestock animals (dugbe virus, nairobi sheep disease virus (nsdv)). nsdv is found in large parts of east africa and the indian subcontinent (where it is known as ganjam virus). we have investigated the ability of nsdv to antagonise the induction and actions of interferon. both pathogenic and apathogenic isolates could actively inhibit the induction of type 1 i ...	2011	22163042
crimean-congo hemorrhagic fever virus-infected hepatocytes induce er-stress and apoptosis crosstalk.	crimean-congo hemorrhagic fever virus (cchfv) is a widely distributed tick-borne member of the nairovirus genus (bunyaviridae) with a high mortality rate in humans. cchfv induces a severe disease in infected patients that includes, among other symptoms, massive liver necrosis and failure. the interaction between liver cells and cchfv is therefore important for understanding the pathogenesis of this disease. here, we described the in vitro cchfv-infection and -replication in the hepatocyte cell l ...	2012	22238639
genetic characterization of erve virus, a european nairovirus distantly related to crimean-congo hemorrhagic fever virus.	erve virus (ervev) is a european nairovirus that is suspected to cause severe headache (thunderclap headache) and intracerebral hemorrhage. the mode of transmission to humans (ticks or mosquitoes) is still unknown. currently, no standardized testing method for ervev exists and only a small partial sequence of the polymerase gene is available. here, we present the first complete genome sequence of ervev s, m, and l segments. phylogenetic comparison of the amino acid sequence of the l-protein (rna ...	2012	22864548
dugbe virus ovarian tumour domain interferes with ubiquitin/isg15-regulated innate immune cell signalling.	the ovarian tumour (otu) domain of the nairovirus l protein has been shown to remove ubiquitin and interferon-stimulated gene 15 protein (isg15) from host cell proteins, which is expected to have multiple effects on cell signalling pathways. we have confirmed that the otu domain from the l protein of the apathogenic nairovirus dugbe virus has deubiquitinating and deisgylating activity and shown that, when expressed in cells, it is highly effective at blocking the tnf-α/nf-κb and interferon/jak/s ...	2012	23136361
diversity of ubiquitin and isg15 specificity among nairoviruses' viral ovarian tumor domain proteases.	nairoviruses are responsible for numerous diseases that affect both humans and animal. recent work has implicated the viral ovarian tumor domain (votu) as a possible nairovirus virulence factor due to its ability to edit ubiquitin (ub) bound to cellular proteins and, at least in the case of crimean-congo hemorrhagic fever virus (cchfv), to cleave the ub-like protein interferon-stimulated gene 15 (isg15), a protein involved in the regulation of host immunity. the prospective roles of votus in imm ...	2013	23345508
ticks and tick-borne viruses from livestock hosts in arid and semiarid regions of the eastern and northeastern parts of kenya.	biodiversity and relative abundance of ticks and associated arboviruses in garissa (northeastern) and isiolo (eastern) provinces of kenya were evaluated. ticks were collected from livestock, identified to species, pooled, and processed for virus isolation. in garissa, rhipicephalus pulchellus gerstacker (57.8%) and hyalomma truncatum koch (27.8%) were the most abundant species sampled, whereas r. pulchellus (80.4%) and amblyomma gemma donitz (9.6%) were the most abundant in isiolo. forty-four vi ...	2014	24605478
[genetic characterization of the sakhalin virus (sakv), paramushir virus (pmrv) (sakhalin group, nairovirus, bunyaviridae), and rukutama virus (rukv) (uukuniemi group, phlebovirus, bunyaviridae) isolated from the obligate parasites of the colonial sea-birds ticks ixodes (ceratixodes) uriae, white 1852 and i. signatus birulya, 1895 in the water area of sea of the okhotsk and bering sea].	full-length genomes of the sakhalin virus (sakh) and paramushir virus (prmv) (sakhalin group, nairovirus, bunyaviridae) isolated from the ticks ixodes uriae white 1852 were sequenced using the next-generation sequencing (genbank id: kf801659, kf801656). sakv and prmv have 81% identity for the part of rna-dependent rna-polymerase (rdrp) on the nucleotide level and 98.5% on the amino acid level. full-length genome comparison shows that sakv have, in average, from 25% (n-protein, s-segment) to 50% ...	2016	25335413
[taxonomy of previously unclassified tamdy virus (tamv) (bunyaviridae, nairovirus) isolated from the hyalomma asiaticum asiaticum schülce et schlottke, 1929 (ixodidae, hyalomminae) in the middle east and transcaucasia].	complete genome sequencing of three tamdy (tamv) virus strains was carried out. the prototype strain tamv/leiv-1308uz was isolated for the very first time from the hyalomma asiaticum asiaticum schülce et schlottke, 1929 (ixodidae, hyalomminae) collected in the august 1971 from sheep in the arid area near namdybulak town (41 degrees 36' n, 64 degrees 39' e) in the tamdinsky district of the bukhara region (uzbekistan). tamv was revealed to be a prototype member of the new phylogenetic group within ...	2016	25069280
detection of a novel putative phlebovirus and first isolation of dugbe virus from ticks in accra, ghana.	ticks are ectoparasites that transmit various types of human and animal pathogens. in particular, emerging and re-emerging diseases caused by tick-borne viruses are public health concerns around the world. however, in many countries of the sub-saharan african region, epidemiological information on tick-borne viral infections is limited, and their prevalence and distribution remain largely unknown. in this study, we conducted surveillance on ticks to detect medically important tick-borne bunyavir ...	2017	28479064