Publications

prokaryotic expression and potential application of the truncated pcv-2 capsid protein.	three pairs of specific primers were designed to amplify the f2-1, f2-2 and xf2-2 truncated sequences of orf2 which encodes the capsid protein of porcine circovirus type 2 (pcv-2). the f2-1 sequence had most of the nls region of orf2, but the f2-2 and xf2-2 genes had the nls region deleted. truncated genes were subcloned into pet-32a(+) vectors to construct recombinant fusion expression vectors. the vectors were then transformed into rosetta(de3) e. coli and expressed by induction of iptg. expre ...	2010	20960305
complete genome sequence of porcine circovirus 2b strain shandong.	shandong is a porcine circovirus 2b (pcv2b) strain that was isolated and purified from tissue samples from pigs with postweaning multisystemic wasting syndrome (pmws) in the shandong province of china. here, we report the complete genome sequence of strain shandong, which may aid in understanding the molecular characteristics of this strain.	2012	23166277
phylogenetic analysis of 32 porcine circovirus type 2 isolates from shandong, china.	porcine circovirus type 2 (pcv2), the essential causative agent of postweaning multisystemic wasting syndrome (pmws), can be divided into distinct genotypes. thirty-two pcv2 isolates obtained made from pigs in shandong province between 2005 and 2013. complete genome sequences were obtained in three replicates for each virus isolate, and the sequences were submitted to the ncbi database. the orf1-encoded amino acid sequences had 98.4 %-100 % identity among the 32 isolates, and there were no signi ...	2015	25488291
genetic analysis of porcine circovirus type 2 from dead minks.	circovirus infection is a growing problem in the field of veterinary and public health. it is associated with enteric diseases in both mammalian and avian hosts. in this study, we detected and isolated porcine circovirus strains in the tissue samples of minks that died from diarrhoea in shandong province, china. we sequenced the whole genome of two porcine strains of circovirus, designated as misd-1 and misd-2， which had a 97.34% similarity on nucleotide sequence and were closely related to porc ...	2016	27324162
the occurrence of porcine circovirus 3 without clinical infection signs in shandong province.	porcine circovirus type 3 (pcv3) was detected in shandong, china. one hundred and thirty-two of 222 (59.46%) samples were pcv3 positive, while 52 of 132 (39.39%) samples were co-infected with pcv2. there were no clinical signs of infection in either multiparous sows or live-born infants. two strains of pcv3 were indentified from natural stillborn foetuses. phylogenetic analysis showed the two strains of pcv3 are 96% identical to the known pcv3/pig/usa (kx778720.1, kx966193.1 and kx898030.1) and ...	2017	28653486