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sybr(®) green-based real-time quantitative reverse-transcription pcr for detection and discrimination of grapevine viruses.a sybr(®) green-based real-time quantitative reverse transcription pcr (qrt-pcr) assay in combination with melt-curve analysis (mca) was optimized for the detection of nine grapevine viruses. the detection limits for simplex qrt-pcr for all nine grapevine viruses were estimated to be in the range of 214-1112 copies of the virus genome. amplicons with melting temperatures (tm) separated by at least 2°c in the mca could differentiate two viruses in the same reaction. therefore, eight of the nine v ...201627246908
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