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a method for the purification of leishmania promastigotes from infected phlebotomine sandflies.we describe a method for the purification of leishmania promastigotes, isolated from infected sandflies (lutzomyia longipalpis) using a discontinuous density centrifugation gradient (percoll/homem). the sandflies, infected seven days previously with leishmania donovani chagasi or leishmania mexicana mexicana from culture, were homogenized and centrifuged on a percoll discontinuous gradient. five interface bands were formed, and most of the promastigotes settled out at the interface between the ( ...19873675038
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