Publications

biosynthesis of brefeldin a. introduction of oxygen at the c-7 position.	the biosynthetic introduction of oxygen in position 7 of brefeldin a, a structurally unique macrolide with an alicyclic ring, was studied. [4-2h]brefeldin c was prepared efficiently from brefeldin a. a high incorporation ratio of the labeled brefeldin c into brefeldin a by eupenicillium brefeldianum clearly indicates that the oxygen in position 7 of brefeldin a does not contribute to the cyclopentane ring formation but is introduced during the last step of brefeldin a biosynthesis.	1983	6678912
immunosuppressive effect of bredinin on cell-mediated and humoral immune reactions in experimental animals.	bredinin (br), an imidazole nucleoside isolated from eupenicillium brefeldianum was previously reported to prolong kidney allograft survival in dogs. the immunosuppressive effect of br was studied in experimental animals. in beagles, in vitro responses of lymphocytes stimulated by mitogens or allogeneic cells were suppressed by in vitro br treatment. br, given in vivo, also showed an inhibitory action against development of delayed hypersensitivity reaction to tubercle bacilli in guinea pigs or ...	1983	6829074
bredinin treatment in clinical kidney allografting.	bredinin, a new nucleoside antibiotic isolated from eupenicillium brefeldianum, and steroids were used as immunosuppressive agents in 31 renal transplant patients; 17 of them received grafts from living-related donors and 14 from cadavers. the one-year graft survival rates were 100% in renal recipients from living donors and 69.2% in cadaver kidney recipients. there was no serious complication from the use of these immunosuppressive agents.	1984	6380037
purification and properties of a beta-1,6-glucanase from penicillium brefeldianum.	an inducible endo-beta-1,6-glucanase was purified from penicillium brefeldianum by deae-cellulose, bio-gel p-150 and high-pressure liquid chromatography. the final preparation was essentially free from beta-1,3-glucanase and beta-glucosidase activities. sodium dodecyl sulphate/polyacrylamide-gel electrophoresis revealed one protein band with an mr of 44000. the vmax. and km values were calculated to be 624 units (mumol/min)/mg and 2.78 mg/ml respectively. the glucanase had lytic activity against ...	1984	6508737
bacterial and fungal cometabolism of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (ddt) and its breakdown products.	resting cells of bacteria grown in the presence of diphenylmethane oxidized substituted analogs such as 4-hydroxydiphenylmethane, bis(4-hydroxyphenyl)methane, bis(4-chlorophenyl)methane (ddm), benzhydrol, and 4,4'-dichlorobenzhydrol. resting cells of bacteria grown with benzhydrol as the sole carbon source oxidized substituted benzhydrols such as 4-chlorobenzhydrol, 4,4'-dichlorobenzhydrol, and other metabolites of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (ddt), such as ddm and bis(4-chloro ...	1985	3994362
analysis of brefeldin a in plasma by gas chromatography with electron capture detection.	the national cancer institute (nci) is pursuing preclinical development of brefeldin a (bfa), a macrolide isolated from penicillium brefeldianum, as an antitumor agent. bfa exhibits a unique spectrum of activity against the human tumor cell line panel that composes the nci's recently established in vitro antitumor screen. a specific method to assay the compound in biological fluids has been developed in which bfa and 1-eicosanol, added as the internal standard, are extracted from plasma specimen ...	1993	8323029
eupenifeldin, a novel cytotoxic bistropolone from eupenicillium brefeldianum.	eupenifeldin was isolated from cultures of eupenicillium brefeldianum atcc 74184 by extraction and crystallization. the compound was identified as a pentacyclic bistropolone on the basis of spectral data and its complete structure was established by single-crystal x-ray analysis. the compound is cytotoxic against the hct-116 cell line and has in vivo antitumor activity in the p388 leukemia model.	1993	8360103
heat-resistant fungi of importance to the food and beverage industry.	spoilage of pasteurized and canned fruit and fruit products caused by heat-resistant molds have been reported repeatedly in recent years. species most commonly implicated in fruit and fruit product disintegration are byssochlamys fulva, byssochlamys nivea, neosartorya fischeri, talaromyces flavus, and eupenicillium brefeldianum. these organisms are saprophytic rather than parasitic and usually contaminate fruits on or near the ground. they can survive heat treatments used for fruit processing an ...	1994	7857517
secondary metabolites by chemical screening, 26.1 7-o-beta-d-galactosyl-brefeldin a via transglycosylation with penicillium brefeldianum.	a lactose-containing fermentation medium initiated the formation of 7-o-beta-d-galactosyl-brefeldin a [1] in the brefeldin a-producing organism, penicillium brefeldianum. the detection, isolation, and physicochemical properties of 1, which has been produced by biological derivatization via transglycosylation, are described. compound 1 is the first glycosylated brefeldin a derivative to have been prepared.	1994	8021656
production of brefeldin-a.	fermentation conditions are described for the production of the antitumor antibiotic 7-(s)-brefeldin-a (brefeldin-a) in liquid culture by eupenicillium brefeldianum, (b.dodge) stolk and scott, atcc 58665. an analytical hplc method was developed which allowed rapid quantitation of the compound during fermentation. a kilogram of brefeldin-a was isolated from a fermentation at the 6800-liter scale.	1995	7662298
mizoribine and mycophenolate mofetil.	both mizoribine (mzr) and mycophenolate mofetil (mmf) are immunosuppressive agents that inhibit the proliferation of lymphocytes selectively, via inhibition of impdh. mzr is a nucleoside of the imidazole class, isolated from the culture medium of the mold eupenicillium brefeldianum m-2166. although this compound has been found to have weak antimicrobial activity against candida albicans, it has proved ineffective against experimental candidiasis. unlike azathioprine, this compound is not taken u ...	1999	10390602
cloning and characterization of saponin hydrolases from aspergillus oryzae and eupenicillium brefeldianum.	we purified saponin hydrolases from aspergillus oryzae pf1224 and eupenicillium brefeldianum pf1226. it was confirmed that the enzymes from a. oryzae pf1224 (sda1) and e. brefeldianum pf1226 (sde1) are glycoproteins with molecular masses of 82 and 90 kda respectively. the deduced amino acid sequences of each enzyme from the cloned genes (sda1 or sde1) showed approximately 50% homology with that of the saponin hydrolase sdn1 from neocosmospora vasinfecta var. vasinfecta pf1225 (ddbj accession no. ...	2005	16306700
penicillium dravuni, a new marine-derived species from an alga in fiji.	penicillium dravuni is a new monoverticillate, sclerotium-forming species that was isolated from the alga dictyosphaeria versluyii collected in dravuni, fiji. this species morphologically is similar to p. turbatum in the p. turbatum subseries of the p. thomii series of the monoverticillata. the nuclear ribosomal internal transcribed spacer region exhibited 97% sequence similarity to known penicillium spp. in the genbank database. phylogenetic analyses revealed that p. dravuni is related most clo ...	2005	16396352
prenylated indolediketopiperazine peroxides and related homologues from the marine sediment-derived fungus penicillium brefeldianum sd-273.	three new indolediketopiperazine peroxides, namely, 24-hydroxyverruculogen (1), 26-hydroxyverruculogen (2), and 13-o-prenyl-26-hydroxyverruculogen (3), along with four known homologues (4-7), were isolated and identified from the culture extract of the marine sediment-derived fungus penicillium brefeldianum sd-273. their structures were determined based on the extensive spectroscopic analysis and compound 1 was confirmed by x-ray crystallographic analysis. the absolute configuration of compounds ...	2014	24473173