Publications

a novel insect-specific flavivirus replicates only in aedes-derived cells and persists at high prevalence in wild aedes vigilax populations in sydney, australia.	to date, insect-specific flaviviruses (isfs) have only been isolated from mosquitoes and increasing evidence suggests that isfs may affect the transmission of pathogenic flaviviruses. to investigate the diversity and prevalence of isfs in australian mosquitoes, samples from various regions were screened for flaviviruses by elisa and rt-pcr. thirty-eight pools of aedes vigilax from sydney in 2007 yielded isolates of a novel flavivirus, named parramatta river virus (parv). sequencing of the viral ...	2015	26519596
complete genome sequence of xishuangbanna flavivirus, a novel mosquito-specific flavivirus from china.	a new flavivirus, xishuangbanna flavivirus (xfv), infecting aedes albopictus mosquitoes in yunnan province, china, was isolated and sequenced. the single-stranded rna genome of 10,884 nt contained two open reading frames (orfs) encoding the polyprotein and fifo. the genome had a maximum nucleotide sequence identity of 65 % to parramatta river virus with coverage of only 27 %. phylogenetic analysis suggested that this virus is most closely related to recognized classical insect-specific flaviviru ...	2016	27001304
infectious dnas derived from insect-specific flavivirus genomes enable identification of pre- and post-entry host restrictions in vertebrate cells.	flaviviruses such as west nile virus (wnv), dengue virus and zika virus are mosquito-borne pathogens that cause significant human diseases. a novel group of insect-specific flaviviruses (isfs), which only replicate in mosquitoes, have also been identified. however, little is known about the mechanisms of isf host restriction. we report the generation of infectious cdna from two australian isfs, parramatta river virus (parv) and palm creek virus (pcv). using circular polymerase extension cloning ...	2017	28592864