Publications

molecular detection and characterization of dichelobacter nodosus in ovine footrot in india.	dichelobacter nodosus was detected in three clinical cases of ovine footrot in kashmir, india. the detection was done by pcr in three clinical specimens directly, without isolating the organism, using species-specific 16s rdna primers. positive results were indicated by amplification of a 783 bp product. all the three samples were subjected to serogrouping by multiplex pcr using group (a-i) specific primers. all the three samples revealed the presence of serogroup b of d. nodosus by yielding a s ...	2004	15294315
isolation and characterization of dichelobacter nodosus from ovine and caprine footrot in kashmir, india.	footrot is a highly contagious and economically important disease of sheep and goats, caused by dichelobacter nodosus, a slow growing anaerobic gram-negative rod. the current australian antigenic classification system, based on variation in the fimbriae, classifies d. nodosus into at least 10 serogroups (a-i and m) and 18 serotypes. this investigation was intended to determine the serological diversity of d. nodosus in this region of kashmir, india. exudates of footrot lesions were collected fro ...	2007	17250860
footrot on a sheep breeding farm in the himalayan state of jammu and kashmir.	in the present study ovine footrot was detected clinically on a sheep farm in the himalayan state of jammu and kashmir. dichelobacter nodosus was confirmed by culture and polymerase chain reaction (pcr) using species-specific 16s ribosomal rna primers. when cultured, the organism appeared as flat colourless colonies having a fine granulated structure with irregular margins, and showing characteristic gram-negative rods with swollen ends. detection by pcr from cultured bacteria resulted in amplif ...	2010	21309465
determination of prevalence and economic impact of ovine footrot in central kashmir india with isolation and molecular characterization of dichelobacter nodosus.	the present study determines the prevalence, economic impact of virulent footrot in central kashmir, india, along with isolation and molecular characterization of dichelobacter nodosus (d. nodosus) where so far no such work has been carried out. over all 12.54% prevalence of footrot was recorded in central kashmir with highest (15.84%) in district srinagar, and least (10.89%) in district budgam, while it was 13.28% in district ganderbal. overall economic impact of footrot was estimated to the tu ...	2011	21397709
non-specificity of primers used for pcr based serogrouping of dichelobacter nodosus and identification of a novel d. nodosus strain.	the present study records the first case of non-specificity of typing primers developed by dhungyel et al. a strain of dichelobacter nodosus (jks-20g) isolated from ovine footrot in kashmir, india, showed specificity for serogroup c and g primers. the fima sequence of the strain turned out to be closer to serogroup g than c. the nucleotide sequence showed maximum homology of 92% with that of serotype g1 strain 238 and 95% with partial sequence available for serotype g2 strain vcs 1004. however, ...	2013	23542117
infectious lameness among migratory sheep and goats in north-west india, with particular focus on anaerobes.	various bacterial species, particularly dichelobacter nodosus, fusobacterium necrophorum, trueperella pyogenes (previously arcanobacterium pyogenes) and treponema spp., have been implicated in infectious conditions manifesting in lameness in sheep and goat populations. the current study reports the causes of infectious lameness in the north-western himalayan region, particularly himachal pradesh (hp), where no such study has been conducted in the past. among a total surveyed population of 27,586 ...	2015	27044157
the detection of dichelobacter nodosus and fusobacterium necrophorum from ovine footrot in kashmir, india.	in a study conducted, a total of 450 swab samples from footrot lesions of naturally infected sheep were collected in all the ten districts of the kashmir valley and were examined for the presence of dichelobacter nodosus (d. nodosus) and fusobacterium necrophorum (f. necrophorum), in order to determine if f. necrophorum was associated with ovine footrot. the detection of f. necrophorum and d. nodosus was carried out by polymerase chain reaction targeting the leukotoxin (lkta) and 16s rrna genes, ...	2015	26159406