Publications

prrs syndrome in quebec: isolation of a virus serologically related to lelystad virus.		1992	1314445
detection of porcine reproductive and respiratory syndrome virus and efficient differentiation between canadian and european strains by reverse transcription and pcr amplification.	two sets of oligonucleotide primers (1008ps-1009pr and 1010pls-1011plr) were designed according to the sequence of the nucleocapsid protein (n) gene of quebec reference strain iaf-exp91 of porcine reproductive and respiratory syndrome virus (prrsv). the primers were used in reverse transcription and pcr (rt-pcr) experiments for detection of viral genomic rna either from infected porcine alveolar macrophages (pam) or tissues from experimentally infected specific-pathogen-free pigs. considering th ...	1994	7814546
antigenic comparison of canadian and us isolates of porcine reproductive and respiratory syndrome virus using monoclonal antibodies to the nucleocapsid protein.	fifteen canadian field isolates of porcine reproductive and respiratory syndrome (prrs) virus from quebec and ontario were compared with 5 us prrs virus (prrsv) isolates and with the european lelystad isolate using monoclonal antibodies (mabs) sdow17, ep147, and vo17 directed to the 15-kda nucleocapsid protein of prrsv. all canadian and us isolates tested by indirect immunofluorescence were recognized by the 3 mabs, and individual titers of mabs towards canadian and us prrsv isolates were simila ...	1995	8521358
structural gene analysis of a quebec reference strain or porcine reproductive and respiratory syndrome virus (prrsv).	the 3' end genomic region of a québec prrsv reference strain (iaf-exp91), propagated in porcine alveolar macrophages (pam), was sequenced and compared to the prototype european strain, the lelystad virus (lv). the sequence, which represents the 3'-terminal 2834 nucleotides, encompassed 5 orfs corresponding to orfs 3 to 7 of lv. extensive genomic variations resulting from an important rate of nucleotide additions, substitutions, and deletions were demonstrated between the two viruses. indeed, the ...	1995	8830492
molecular analysis of the orfs 3 to 7 of porcine reproductive and respiratory syndrome virus, québec reference strain.	the cdna sequence of the 3'-terminal genomic region of the québec iaf-exp91 strain of porcine reproductive and respiratory syndrome virus (prrsv) was determined and compared to those of other reference strains from europe (lelystad virus) and us (atcc vr2385, mn-1b). the sequence (2834 nucleotides) which encompassed orfs 3 to 7 revealed extensive genomic variations between the québec strain and lelystad virus (lv), resulting from high number of base substitutions, additions and deletions. the or ...	1995	7661693