Publications

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detection of rna from a novel west nile-like virus and high prevalence of an insect-specific flavivirus in mosquitoes in the yucatan peninsula of mexico.as part of our ongoing surveillance efforts for west nile virus (wnv) in the yucatan peninsula of mexico, 96,687 mosquitoes collected from january through december 2007 were assayed by virus isolation in mammalian cells. three mosquito pools caused cytopathic effect. two isolates were orthobunyaviruses (cache valley virus and kairi virus) and the identity of the third infectious agent was not determined. a subset of mosquitoes was also tested by reverse transcription-polymerase chain reaction (r ...200919141845
detection of flaviviruses and orthobunyaviruses in mosquitoes in the yucatan peninsula of mexico in 2008.a total of 191,244 mosquitoes from 24 species were collected in the yucatan peninsula of mexico from january to december 2008, and tested for the presence of cytopathic virus by virus isolation in vero cells. eighteen virus isolates were obtained, all of which were orthobunyaviruses. these were identified by reverse transcription-polymerase chain reaction (rt-pcr) and nucleotide sequencing as cache valley virus (n=17) and south river virus (n=1). a subset (n=20,124) of culex quinquefasciatus col ...201020370430
orthobunyaviruses, a common cause of infection of livestock in the yucatan peninsula of mexico.to determine the seroprevalence of selected orthobunyaviruses in livestock in the yucatan peninsula of mexico, a serologic investigation was performed using serum samples from 256 domestic animals (182 horses, 31 sheep, 1 dog, 37 chickens, and 5 turkeys). all serum samples were examined by plaque reduction neutralization test using cache valley virus (cvv), cholul virus (chlv), south river virus (sourv), kairi virus, maguari virus, and wyeomyia virus. of the 182 horses, 60 (33.0%) were seroposit ...201223045250
sequence and phylogenetic data indicate that an orthobunyavirus recently detected in the yucatan peninsula of mexico is a novel reassortant of potosi and cache valley viruses.we determined the complete nucleotide sequences of the small (s) and medium (m) rna segments of an orthobunyavirus isolated from mosquitoes in the yucatan peninsula of mexico. a 528-nt region of the large (l) rna segment was also sequenced. the s rna segment has greatest nucleotide identity to the homologous region of cache valley virus (cvv; 98%) followed by potosi virus (potv; 89%) and northway virus (86%). the m rna segment has 96% nucleotide identity to the homologous region of potv, and les ...201222407405
nucleotide sequencing and serologic analysis of cache valley virus isolates from the yucatan peninsula of mexico.nucleotide sequencing was performed on part of the medium and large genome segments of 17 cache valley virus (cvv) isolates from the yucatan peninsula of mexico. alignment of these sequences to all other sequences in the genbank database revealed that they have greatest nucleotide identity (97-98 %) with the equivalent regions of tlacotalpan virus (tlav), which is considered to be a variety of cvv. next, cross-plaque reduction neutralization tests (prnts) were performed using sera from mice that ...201222467180
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