Publications

molecular epidemiology of sars-associated coronavirus, beijing.	single nucleotide variations (snvs) at 5 loci (17564, 21721, 22222, 23823, and 27827) were used to define the molecular epidemiologic characteristics of severe acute respiratory syndrome-associated coronavirus (sars-cov) from beijing patients. five fragments targeted at the snv loci were amplified directly from clinical samples by using reverse transcription-polymerase chain reaction (rt-pcr), before sequencing the amplified products. analyses of 45 sequences obtained from 29 patients showed tha ...	2005	16229772
serotype 1-specific monoclonal antibody-based antigen capture immunoassay for detection of circulating nonstructural protein ns1: implications for early diagnosis and serotyping of dengue virus infections.	rapid diagnosis and serotyping of dengue virus (dv) infections are important for timely clinical management and epidemiological control in areas where multiple flaviviruses are endemic. however, the speed and accuracy of diagnosis must be balanced against test cost and availability, especially in developing countries. we developed a specific antigen capture enzyme-linked immunosorbent assay (elisa) for early detection and serotyping of dv serotype 1 (dv1) by using well-characterized monoclonal a ...	2006	16891505
real-time pcr for detecting circulating dengue virus in the guangdong province of china in 2006.	dengue virus (denv) causes a wide range of diseases in humans, from the acute febrile illness dengue fever (df) to life-threatening dengue haemorrhagic fever/dengue shock syndrome. we developed four real-time quantitative pcr assays for each serotype of denv based on computational analysis. these assays had high sensitivity and specificity without cross-reactivity for the four serotypes. to evaluate the performance of these assays in detecting and typing the virus in clinical samples, we analyse ...	2008	19018028