Publications

use of a sandwich-enzyme-linked immunosorbent assay (sea) for the diagnosis of natural fasciola hepatica infection in cattle from galicia (nw spain).	an indirect-enzyme immune-linked immunosorbent assay (iea) was compared to a sandwich-enzyme-linked immunosorbent assay (sea) to diagnose fasciolosis in naturally infected cattle. by means of a rabbit polyclonal igg antibody to f. hepatica antigens, captured circulating antigens were detected by an sea technique. a total of 85.1% of the samples examined was considered positive by using the iea-test, whereas this percentage was of 37.3% by the sea test. using the two enzymatic probes, only the 38 ...	2000	11027859
influence of age and breed on natural bovine fasciolosis in an endemic area (galicia, nw spain).	an analysis was undertaken of the effect of breed and age on bovine fasciolosis using antibody and antigen detection elisas. a total of 84.3% of the 1284 serum samples examined had positive antibody values and 20.4% exhibited antigenaemia. the seroprevalence of antibodies in crossbred cattle was higher than that in autochthonous rubia gallega, friesian or brown swiss cows. the highest percentage antigenaemia occurred in the brown swiss cattle, but the breed differences were not statistically sig ...	2002	12212726
prevalence of natural ovine fasciolosis shown by demonstrating the presence of serum circulating antigens.	the prevalence of fasciolosis in sheep (galicia, northwest spain) kept under field conditions was determined by using a sandwich-enzyme-linked immunosorbent assay (selisa). serum fasciola hepatica circulating antigens were captured by means of a rabbit polyclonal igg antibody to f. hepatica excretory/secretory products. results were compared to those obtained by faecal sedimentation and an indirect elisa (ielisa) and excretory/secretory antigens. prevalences were 39.1% by selisa, 30.4% by faecal ...	2003	14574565
evaluation of the flukicide treatment policy for dairy cattle in galicia (nw spain).	fasciola hepatica infection is an important cause of lost productivity in livestock worldwide. effective control of fasciolosis is difficult, especially in milking cows, which can only be treated during dry periods, a control strategy that has not been yet evaluated. in this cross-sectional study, we investigated the effect of the type of flukicide treatment on the prevalence and intensity of infection in dairy cattle from galicia, an area where fasciolosis is endemic and which is also the main ...	2008	18774648
relationships between eosinophilia, anti-fasciola igg, and igm rheumatoid factors, in urban and rural areas of north-western spain.	a study was carried out, among adult patients attending a hospital in lugo, in north-western spain, to investigate possible relationships between eosinophilia, igg antibodies against the parasitic helminth fasciola hepatica, and igm rheumatoid factors (igm-rf). blood samples were collected from 1264 individuals and divided into three groups according to eosinophil count: normal (<0.5 x 10(9) eosinophils/litre), eosinophilic (0.5 x 10(9)-3 x 10(9)/litre) or hyper-eosinophilic (>3 x 10(9)/litre). ...	2008	18782488
the wild boar (sus scrofa linnaeus, 1758) as secondary reservoir of fasciola hepatica in galicia (nw spain).	fasciolosis is an emerging or reemerging human and animal disease in numerous parts of the world. in galicia (nw, spain), the wild boar (sus scrofa) is the main wild ungulate in terms of abundance and distribution. its population has continuously increased over the past decades and this population growth has been accompanied by a reduction of habitats, so that the wild boar populations encroach more and more frequently onto agricultural lands. the increase of the interface area between livestock ...	2013	24103736
temporal genetic variation of fasciola hepatica from sheep in galicia (nw spain).	we found low genetic differentiation between two temporal samples of fasciola hepatica (2006 and 2008) collected from nine sheep of the same flock that shared the same pasture for at least 2 years. however, each sample, represented by four and five infrapopulations respectively, showed strong heterozygote deficits regarding hardy-weinberg expectations and a high degree of genetic structure at infrapopulation level. this is an unexpected result since genetic drift should increase temporal variati ...	2015	25825147
transmission of calicophoron daubneyi and fasciola hepatica in galicia (spain): temporal follow-up in the intermediate and definitive hosts.	paramphistomosis caused by calicophoron daubneyi and fasciolosis caused by fasciola hepatica are common parasitic diseases of livestock animals. transmission of the diseases depends on the presence of intermediate hosts, i.e. freshwater gastropods such as lymnaeids. we carried out a 2-year-long study of the dynamics of the snail population acting as the intermediate host for these parasites, considering the population structure in terms of size/age and infection status. in addition, we determine ...	2016	27894356