Publications

multilocus enzyme electrophoresis and cytochrome b gene sequencing-based identification of leishmania isolates from different foci of cutaneous leishmaniasis in pakistan.	seventeen leishmania stocks isolated from cutaneous lesions of pakistani patients were studied by multilocus enzyme electrophoresis and by polymerase chain reaction amplification and sequencing of the cytochrome b (cyt b) gene. eleven stocks that expressed nine zymodemes were assigned to l. (leishmania) major. all of them were isolated from patients in the lowlands of larkana district and sibi city in sindh and balochistan provinces, respectively. the remaining six, distributed in two zymodemes ...	2006	16896129
polymorphisms of cytochrome b gene in leishmania parasites and their relation to types of cutaneous leishmaniasis lesions in pakistan.	the exact species and/or strains of leishmania parasites involved strongly influence the clinical and epidemiological features of leishmaniasis, and current knowledge of those influences and relationships is inadequate. we report that cytochrome b (cyt b) gene sequencing identified causal leishmania parasites of 69 cutaneous leishmaniasis cases in pakistan over a 3-year period. of 21 cases in highland areas (quetta city, balochistan province), 16 (76.2%) were identified as leishmania (l.) tropic ...	2008	18271802
cutaneous leishmaniasis caused by leishmania (l.) major infection in sindh province, pakistan.	leishmaniasis is endemic in pakistan and is wide-spread throughout the country. polymerase chain reaction (pcr) was performed to identify the leishmania species present in cutaneous leishmaniasis (cl) patients from new endemic areas of the central part of sindh province, pakistan. the pcr primers used were designed for the identification and differentiation of leishmania (leishmania) major and leishmania (leishmania) tropica species, and pcr bands at 620 and 830 bp of the parasite-specific kinet ...	2009	19467219
zoophilic feeding behaviour of phlebotomine sand flies in the endemic areas of cutaneous leishmaniasis of sindh province, pakistan.	leishmania (leishmania) major has been identified as the major causative agent of cutaneous leishmaniasis in sindh province of southern pakistan. to make a rational approach for understanding the pathogen transmission cycles, the sand fly species and their natural blood meals in the endemic areas were examined. total dna was individually extracted from sand flies collected in four villages in sindh province. pcr-rflp (restriction fragment length polymorphism) and sequence analysis of the 18s rib ...	2012	22246369
population genetics of leishmania (leishmania) major dna isolated from cutaneous leishmaniasis patients in pakistan based on multilocus microsatellite typing.	cutaneous leishmaniasis (cl) is a major and fast increasing public health problem, both among the local pakistani populations and the afghan refugees in camps. leishmania (leishmania) major is one of the etiological agents responsible for cl in pakistan. genetic variability and population structure have been investigated for 66 dna samples of l. (l.) major isolated from skin biopsy of cl patients.	2014	25030377