Publications

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dynamics of the elimination of dicrocoelium dendriticum (trematoda, digenea) eggs in the faeces of lambs and ewes in the porma basin (león, nw spain).the elimination of dicrocoelium dendriticum eggs in sheep faeces from 4 localities in the upper and middle porma river basin was recorded at monthly intervals between march 1986 and march 1987. the faecal material was taken directly from the rectum of the animals (10% of the flock in each village). the sedimentation technique and mcmaster egg counting chambers were used to analyze the faecal samples. of the 995 samples examined, 63.6% contained d. dendriticum eggs and the number of eggs per gram ...19911952698
coprological study of the dicrocoelium dendriticum (digenea) egg elimination by cattle in highland areas in león province, northwest spain.dicrocoelium dendriticum egg output in cattle from five locations of the porma river basin (león province, northwest spain) was studied at monthly intervals between march 1986 and march 1987. we found d. dendriticum eggs in 37.64% of the 1251 samples examined, and the number of eggs per gram (epg) ranged from 10 to 1000 (average, 41.65 +/- 2.73). the main egg-elimination period, for prevalence, was autumn-winter. the average epg values were quite similar during all months except march, when the ...19938415564
seroprevalence of fasciola hepatica infection in sheep in northwestern spain.to estimate the prevalence of fasciola hepatica infection in sheep in the león province (northwestern spain), we conducted a survey between october 1992 and may 1993. a total of 767 samples of feces and serum were collected from sheep over 1 year of age belonging to 152 flocks randomly selected from the 4 natural regions of león province. samples were analyzed by a standard coprological sedimentation method and an indirect enzyme-linked immunosorbent assay (elisa) using excretory-secretory produ ...19957731921
characterization of adult dicrocoelium dendriticum by isoelectric focusing.water soluble extracts of 3131 adult specimens of dicrocoelium dendriticum from cattle, sheep and goats, mainly from león province, were analysed by isoelectric focusing in thin-layer polyacrylamide gels. activity of the following enzymes was studied: lactate dehydrogenase (ldh, ec 1.1.1.27), glucose phosphate isomerase (gpi, ec 5.3.1.9), phosphoglucomutase (pgm, ec 2.7.5.1), acid phosphatase (acp, ec 3.1.3.2), alpha-glycerophosphate dehydrogenase (alpha-gpdh, ec 1.1.1.8), hydroxybutyrate dehydr ...19989687590
contributions to and review of dicrocoeliosis, with special reference to the intermediate hosts of dicrocoelium dendriticum.an epidemiological study on dicrocoeliosis caused by dicrocoelium dendriticum was carried out on sheep, molluscs and ants in the mountains of león province (nw spain) between 1987-1991. the results concerning the intermediate hosts and a review of some aspects of dicrocoeliosis are summarized. mollusc collection for the helminthological study was random throughout the study area at fortnightly intervals. twenty-nine gastropoda species were identified. d. dendriticum infection was only detected i ...200111769295
field and experimental studies on dicrocoelium dendriticum and dicrocoeliasis in northern spain.the transmission, control and the relationship between dicrocoelium dendriticum and its definitive (sheep and cattle) and intermediate (molluscs and ants) hosts under natural and experimental conditions are described. eleven species of molluscs and four of ants were found infected with larval d. dendriticum in león province, north-west spain. infected ants were observed between april and november and in tetania at 7.5-26.9 degrees c. the highest shedding of eggs by sheep and cattle was detected ...200516336711
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