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biochemical characteristics of chitosanase from the indonesian bacillus licheniformis mb-2.bacillus licheniformis mb-2, isolated from a hot spring water in manado, indonesia, secreted a unique chitosanase. media consisted of 0.24% chitosan, 0.25% casiton, 1% mgso4, 1.4% k2hpo4, 0.02% cacl2 x 2h2o, 0.002% feso4 x 7h2o (w/v) was used for enzyme production. purification of the enzyme through the hydrophobic interaction chromatography system (butyl sepharose 4 ff) resulted in two major active fractions; the f2 fraction was shown as a single band at both sodium dodecyl sulfate-polyacrylami ...200616757796
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