Publications

vitrification of yunnan yellow cattle oocytes: work in progress.	the objective of this study was to provide a simple cryopreservation method for oocytes from yunnan yellow cattle and facilitate preservation efforts in this native chinese breed, which is threatened by agricultural modernization. cumulus-oocyte complexes (cocs) were collected from slaughterhouse ovaries and matured in vitro for 22-24 h, then selected for cryopreservation. vitrification in open pulled straws (ops) or in microdrops on a cooled metal surface (solid surface vitrification, ssv) was ...	2002	12387339
genetic diversity and relationship of yunnan native cattle breeds and introduced beef cattle breeds.	in this study, random amplified polymorphic dna (rapd) analysis was used to estimate genetic diversity and relationship in 134 samples belonging to two native cattle breeds from the yunnan province of china (dehong cattle and diqing cattle) and four introduced beef cattle breeds (brahman, simmental, murrygrey, and shorthorn). ten primers were used, and a total of 84 bands were scored, of which 63 bands (75.0%) were polymorphic. the genetic distance matrix was obtained by proportions of shared fr ...	2004	15068334
origin and phylogeographical structure of chinese cattle.	complete mitochondrial d-loop sequences of 231 samples were used to explore the origin and genetic diversity of chinese cattle. phylogenetical analysis of these sequences revealed both bos taurus and bos indicus mitochondrial types in chinese cattle. four of the previously identified mitochondrial dna lineages (t1-t4) were identified in the bos taurus type, including lineage t1, which was found for the first time in chinese cattle. two lineages (i1 and i2) were identified in the bos indicus type ...	2006	17121603
genetic variation of mitochondrial d-loop region and evolution analysis in some chinese cattle breeds.	the complete mitochondrial d-loop region from 123 individuals in 12 chinese cattle breeds and two individuals in germany yellow cattle breed was sequenced and analyzed. the results were shown as follows: 93 variations and 57 haplotypes were detected, and the average number of nucleotide difference was 22.708, nucleotide diversity (d) was 0.0251 +/- 0.00479, and haplotype diversity (hd) was 0.888 +/- 0.026, indicating very high genetic diversity in chinese cattle breeds. in the neighbor-joining t ...	2007	17601610
genetic diversity and origin of gayal and cattle in yunnan revealed by mtdna control region and sry gene sequence variation.	there are hump, humpless cattle and gayal distributed in yunnan province, south-west china, but their genetic background remains unclear. to determine the origin and genetic diversity of yunnan gayal and cattle (diqing, nujiang and wenshan cattle), we analysed mtdna control region sequences of 71 samples and sry gene sequences of 39 samples, together with the available sequences in genbank. the neighbour-joining phylogeny and the reduced median network analysis showed that yunnan gayal originate ...	2010	20433524
dominant bacterial communities in the rumen of gayals (bos frontalis), yaks (bos grunniens) and yunnan yellow cattle (bos taurs) revealed by denaturing gradient gel electrophoresis.	the dominant rumen bacteria in gayals, yaks and yunnan yellow cattle were investigated using pcr-dgge approach. the analysis of dgge profiles, identification of dominant bands and phylogenetic analysis 16s rdna sequences in dgge profiles were combined to reveal the dominant bacterial communities and compared the differences between those cattle species. dgge profiles revealed that gayals had the most abundant dominant bacteria and the lowest similarity of intraspecies between individuals than ot ...	2010	21140219
molecular diversity of bacteria in yunnan yellow cattle (bos taurs) from nujiang region, china.	the rumen content of four yunnan yellow cattle (bos taurs) were collected to determine the bacteria diversity by using 16s rrna gene sequence analysis. a total of 129 sequences were examined and the sequences were referred as 107 otu (operational taxonomy unit) according to the similarity level of 97% in gene sequence. similarity analysis revealed that yunnan yellow cattle had 12 sequences (10 otu) shared 97% or greater similarity with cultured rumen bacteria butyrivibrio fibrisolvens, succinicl ...	2011	21598111
[effects of planting rubber and crops on the keeping number of livestock and poultry in the naban river watershed national nature reserve].	a total of 207 householders from 13 natural villages in the naban river watershed national nature reserve were chosen for a systematic survey on the farming and livestock husbandry, aimed to evaluate the effects of planting rubber and crops on the keeping number of livestock and poultry in the study area. the livestock and poultry bred in the reserve were mainly buffalo, yunnan yellow cattle, small-eared pig, chahua chicken, and a few banna game fowls. exotic breeds were very limited. from 1991 ...	2011	22263476
genetic diversity analysis of bmy cattle based on microsatellite dna markers.	bmy cattle (1/2 brahman, 1/4 murray grey and 1/4 yunnan yellow cattle) has been inter se breeding since 1980s. genetic diversity of bmy cattle was extensively investigated using 16 microsatellite markers. a total of 130 microsatellite alleles and high allele size variance were detected. all loci displayed high genetic diversity with overall mean of n(a) = 8.13, pic = 0.7224 and h(e) = 0.7666, which were higher than those of many other beef breeds. the allele-sharing neighbour-joining tree clearl ...	2012	22730772