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antigenic and genetic diversities of babesia ovata in persistently infected cattle.exploring the antigenic and genetic diversities of babesia ovata, we obtained several field isolates from grazing cattle in the okushiri island, japan. parasite isolation was greatly facilitated by using bovine red blood cell-substituted scid mice (bo-rbc-scid mice), into which the blood samples of the cattle were inoculated. isolates from different individuals within a herd of cattle were compared in immunoblot analysis with an anti-b. ovata serum and also in southern blot analysis with a probe ...19989879533
pcr detection of babesia ovata from questing ticks in japan.babesia ovata is a tick-transmitted hemoprotozoan parasite of cattle. in the present study, we analyzed tick dna samples (n=1459) prepared from questing ticks collected from various cattle pastures in hokkaido (shibecha, taiki, otofuke, memuro, and shin-hidaka districts) and okinawa (yonaguni island) prefectures of japan for b. ovata. when all the tick dna samples were screened by a previously described b. ovata-specific apical membrane antigen-1 (ama-1) gene-based polymerase chain reaction (pcr ...201424572609
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