Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| human cytomegalovirus. assay by counting infected cells. | 1963 | 13949074 | |
| opportunistic infections. human cytomegalovirus infection. | 1965 | 14283887 | |
| spectrum of human cytomegalovirus complement-fixing antigens. | 1967 | 4293994 | |
| isolation of human cytomegalovirus from the circulating leucocytes of a leukaemic patient. | 1968 | 4295969 | |
| [study on cytomegalovirus infection. 2. study on human cytomegalovirus infection in cultured cells]. | 1968 | 4304724 | |
| [injurious effect of 6-azauridine on the multiplication of human cytomegalovirus]. | 1969 | 4320268 | |
| prevalence and duration of postnatally acquired human cytomegalovirus infection. | 1970 | 4314404 | |
| preparation of immune rabbit sera with neutralizing activity against human cytomegalovirus and varicella-zoster virus. | 1970 | 4316778 | |
| indirect haemagglutination of human cytomegalovirus. | 1971 | 4331013 | |
| human cytomegalovirus infection of cultured fibroblasts. ii. viral replicative sequence of a wild and an adapted strain. | 1972 | 4341547 | |
| the development of immunofluorescent antibodies as compared with complement-fixing and virus-neutralizing antibodies in human cytomegalovirus infection. | 1972 | 4341813 | |
| three-day assay for human cytomegalovirus applicable to serum neutralization tests. | a fluorescing cell assay (fca) technique utilizing the indirect fluorescent-antibody method to measure human cytomegalovirus (cmv)-infected cells has been applied to the rapid determination of cmv-neutralizing antibody. human sera with complement fixation titers to cmv of 1/32 or greater and fluorescein-conjugated rabbit anti-human globulin are the primary and secondary reagents in the fluorescent-antibody test. fca measured in 3 days the same number of infectious units measured by plaque assay ... | 1973 | 4346976 |
| cytomegalovirus replication in cells pretreated with 5-iodo-2'-deoxyuridine. | the replication of human cytomegalovirus (cmv) in cells pretreated with 5-iodo-2'-deoxyuridine (iudr) was studied. pretreatment of cells with iudr enhanced several parameters of virus replication. virus grown in drug-treated cells exhibited a shorter eclipse period and the cells produced more infectious virus sooner than did untreated cells. there was an approximate fivefold increase in virus yield per cell in the drug-treated samples when compared to control cultures. the time required for plaq ... | 1973 | 4351463 |
| "plexiform vermicellar arrays" in human cytomegalovirus-infected wi-38 human embryonic fibroblast cells. | 1974 | 4372865 | |
| multiplicity reactivation of human cytomegalovirus inactivated by ultra-violet light. | 1974 | 4374440 | |
| human cytomegalovirus stimulates host cell rna synthesis. | human cytomegalovirus infection of human fibroblast cells (wi-38) induced cellular rna synthesis. the rna synthesis in infected cultures preceded the synthesis of viral dna and progeny virus by approximately 24 h. rna species synthesized in infected cells included ribosomal 28s and 18s; and 4s transfer rna; all were markedly increased in comparison to uninfected cells. this induction of host cell rna synthesis was dependent upon a protein(s) that was synthesized during the early stages of infect ... | 1975 | 163357 |
| partial characterization of a soluble antigen preparation from cells infected with human cytomegalovirus: properties of antisera prepared to the antigen. | soluble antigen (sa) preparations were obtained from cell cultures infected with either the davis or ad169 strains of cytomegalovirus (cmv). fractionation of sa preparations through sephadex g-200 resulted in a molecular weight value ranging from 67,000 to 85,000. rate-zonal centrifugation produced an approximate value of 5.5s for the cmv antigenic material. antisera to sa prepared from either ad169- or davis-infected cells lacked neutralizing activity but produced specific fluorescence confined ... | 1975 | 164507 |
| demonstration of the oncogenic potential of herpes simplex viruses and human cytomegalovirus. | 1975 | 169029 | |
| [late stages in the replicative cycle of human cytomegaloviruses in diploid cells. ultrastructural study]. | an ultrastructural study of late stages of the replicative cycle of human cytomegalovirus (cmv) in human embryo fibroblasts cell cultures has been performed. nucleocapsids are observed only inside the intranuclear inclusion. they mature apparently by taking the envelope from the structural components of microvescicular systems reaching from the nuclear membrane the periphery of the nuclear inclusion. however, some capsids reach "naked" the cytoplasm and then take the envelope from cytoplasmic me ... | 1975 | 180913 |
| cytomegalovirus infection of human lung epithelial cells in vitro. | human lung epithelial cells were productively infected with human cytomegalovirus in vitro. infectious virus was released up to 8 weeks postinfection. the cells retained their morphological characteristics throughout the period of observation, while simultaneously bearing all the features typical of cytomegalovirus infection. | 1975 | 181345 |
| [various ways of acquiring the pericapsule at the nuclear membrane level by herpes simplex virus and human cytomegalovirus]. | 1975 | 182189 | |
| plaque reduction neutralization test for human cytomegalovirus based upon enhanced uptake of neutral red by virus-infected cells. | foci of cells infected with human cytomegalovirus were noted to stain more intensely than uninfected cells with neutral red, and this provided the basis for development of a plaque assay and plaque reduction neutralization test for cytomegalovirus. plaques demonstrable by neutral red staining could be counted at 8 days after infection; thus, results could be obtained earlier than for plaque assay systems based upon the viral cytopathic effect, a fewer manipulations were required for staining cel ... | 1976 | 182716 |
| structural proteins of human cytomegalovirus. | 1976 | 183384 | |
| comparative activity of immunofluorescent antibody and complement-fixing antibody in cytomegalovirus infection. | three different tests for detection of antibodies to human cytomegalovirus (cmv), complement fixing with antigen prepared by freeze-thaw disruption (cf-ft) or with antigen prepared by extraction with alkaline glycine buffer (cf-ge) and immunofluorescent staining (fa), were compared in renal transplant recipients and their healthy donors, fa and cf-ge tests yielded positive results at an identical and significantly higher frequency than cf-ft in both donors and recipients. cf-ge and fa performed ... | 1976 | 184108 |
| induction of murine p30 by superinfecting herpesviruses. | the interaction of endogenous type c viruses with superinfecting herpes simplex virus type 2 (hsv-2) was investigated in two murine cell lines. replication of hsv-2 was suboptimal in random-bred swiss/3t3a cells and, in initial experiments, infection with a low virus-to-cell ratio resulted in carrier cultures with enhanced murine leukemia virus (mulv) p30 expression. immunofluorescence tests with swiss/3t3a cells productively infected with hsv-2 also showed hsv-associated cytoplasmic antigens an ... | 1976 | 184296 |
| antisera to human cytomegalovirus produced in hamsters: reactivity in radioimmunoassay and other antibody assay systems. | hamsters immunized with human cytomegalovirus (cmv) concentrated and purified by polyethylene glycol precipitation and density gradient centrifugation produced antisera with high titers of specific viral antibody, and which showed no significant reactivity with human host cell components. the antisera had high titers of cmv antibody in complement fixation, indirect fluorescent-antibody (fa), and neutralization tests, but titers obtained by indirect radioimmunoassay (ria) were markedly higher. th ... | 1976 | 185151 |
| [cytomegalic virus infection in normal and disturbed immunity. clinical and animal experiment studies]. | virological and serological studies of cytomegalovirus infections revealed following results: 1. the distribution of cytomegalovirus infections in the area of munich can be compared with other european areas. 57% of adults have antibodies against the cytomegalovirus. 2. cytomegalovirus is an important agent initiating infectious liver diseases in childhood. 3. the virus is also an important factor for severe cerebral retardation. 4. studies in adult mice showed that a non-pathogenic strain of mo ... | 1976 | 186373 |
| latent infection of mouse cells with human cytomegalovirus. | infection of secondary mouse fibroblast cultures with human cytomegalovirus (hcmv) led to the production of viral antigens in the absence of detectable virus replication. antigen production was not dependent on viral dna synthesis since it was not inhibited by cytosine arabinoside. beginning at 15 days post-infection, viral-specific antigens were no longer observable in infected cultures, but could be induced by iododeoxyuridine (iudr) treatment. such cultures carry hcmv genetic information in a ... | 1976 | 187270 |
| thymidine-kinase in cytomegalovirus infected cells. | in human diploid fibroblast lep cells infected with ad169 strain of human cytomegalovirus (cmv) a sharp increase of cytosol thymidine kinase activity was observed. the properties of the cytosol enzymes from infected and non-infected cells were compared. no significant differences between the enzymes from infected and control cells were observed in substrate specificity, ph dependence, thermostability and relative electrophoretic mobility. human sera containing high titres of cmv complement-fixin ... | 1976 | 13768 |
| evidence for early nuclear antigens in cytomegalovirus-infected cells. | human cytomegalovirus (cmv) induces nuclear antigens resembling the epstein-barr nuclear antigen (ebna) as early as 3 h after infection. these early antigens can be detected only with the anti-complement immunofluorescence staining (acif) technique. synthesis of these new antigens is not influenced by cytosine arabinoside (ara-c). | 1976 | 190342 |
| double arc structures in nuclei infected with human cytomegalovirus. | 1976 | 193488 | |
| human cytomegalovirus: a review of developments between 1970 and 1976. part ii. experimental developments. | over the last 6 years, our knowledge of the basic characteristics of human cytomegalovirus (hcmv) has been greatly enhanced, especially as concerns the microbiology of this virus. dna relatedness studies show that there is 80% homology between different strains, regardless of their origin. studies on the relationships between hcmv and host-cell metabolism reveal that it induces both a virus specific and a cellular dna polymerase, stimulates ribosomal rna, induces early protein synthesis and res ... | 1977 | 194635 |
| human cytomegalovirions and dense bodies: glycopeptide analysis and mechanism of cell rounding and polykaryocytosis. | human cytomegalovirions and dense bodies labeled with [14c]glucosamine were analyzed by means of sodium dodecyl sulfate-urea polyacrylamide gel electrophoresis and autoradiography. the same glycopeptide composition was found in both cytomegalovirions and dense bodies. these consisted of at least 11 glycopeptides which ranged in molecular weight from 46,500 to over 170,000 daltons. addition of purified human cytomegalovirus (cmv) at high multiplicity to confluent monolayers of human fibroblasts p ... | 1977 | 200587 |
| human cells transformed in vitro by human cytomegalovirus: tumorigenicity in athymic nude mice. | athymic nude mice were inoculated with human embryo lung cells transformed in vitro by human cytomegalovirus (cmv). of the inoculated animals, 62% developed tumors after an average latent period of 19 days. the tumors were composed of small, polygonal cells with large nulei and scanty cytoplasm embedded in an abundant collagenous matrix. the cells were poorly differentiated but may have been of epithelial origin. adjacent structures were rarely invaded. cmv-related intracellular and membrane ant ... | 1977 | 191621 |
| stimulation of cellular thymidine kinases by human cytomegalovirus. | thymidine kinase (tk) activity in wi-38 and mrc-5 human fibroblasts was analyzed by discontinuous polyacrylamide gel electrophoresis (disc-page) and discontinuous glycerol gradient electrophoresis (disc-gep) after subculture or human cytomegalovirus (hcmv) infection. two peaks of tk activity with different relative fraction-of-migration (r(f)) values were resolved by disc-page or disc-gep in extracts from log-phase and infected cells. growing wi-38 cells expressed a slowly migrating (r(f) = 0.14 ... | 1977 | 20515 |
| complement-fixing antigen of human cytomegaloviruses. | the titer of complement-fixing (cf) antigen of human cytomegalovirus (cmv) strain c87 was not reduced by 10 cycles of freezing and thawing or by storage at 4 c for two months, although the virus was labile at 37 c and 100 c. the rapid increase in titers of cf antigen and plaque-forming units was seen from days 3 through 6 after infection; however, cf antigen was detected 24 hr after infection. the molecular weight of cf antigen was estimated by gel filtration to be greater than 1.5 x 10(7) dalto ... | 1977 | 190330 |
| blastogenic response of human lymphocytes to human cytomegalovirus. | a method was developed for measuring the blastogenic response of human lymphocytes to human cytomegalovirus (cmv). viral and control antigens were prepared by extracting disrupted infected and uninfected cell cultures with an alkaline buffer. lymphocytes from ten donors with complement-fixing (cf) antibody exhibited a blastogenic response, whereas cells from ten seronegative donors did not. a relationship between the stimulation index (si) and the results of neutralization (nt), indirect haemagg ... | 1977 | 203423 |
| antigenic relatedness of 17 strains of human cytomegalovirus. | a 10 minute kinetic neutralization test was used to assess antigenic relatedness among 17 strains of human cytomegalovirus. hyperimmune guinea pig sera exhibited extensive cross-reactivity. however, the data did suggest that there may be four antigenic groups represented in this collection of cmv strains. available epidemiologic data for these strains do not provide any discernible basis for this grouping. | 1978 | 206134 |
| intracellular forms of the parental human cytomegalovirus genome at early stages of the infective process. | 1978 | 208251 | |
| replication of herpesviruses in human cells transformed by cytomegalovirus. | the replication of human cytomegalovirus (cmv) and herpes simplex virus (hsv) was studied in three human embryo cell lines (cmv-mj-hel-i, cmv-mj-hel-2, and cmv-mj-hel-2,t-i) transformed in vitro by human cmv. growth studies revealed that these cells were completely resistant to infection by cmv strains adi69 and mj and partially resistant to hsv types i and 2. neither virus dna nor virus proteins were synthesized in the transformed cells infected with cmv ad169. the hsv production in cmv-transfo ... | 1978 | 211187 |
| human cytomegalovirus-induced chromatin factors responsible for changes in template activity and structure of infected cell chromatin. | 1978 | 214942 | |
| factors affecting the interferon sensitivity of human cytomegalovirus. | several factors affected the interferon sensitivity of human cytomegalovirus in human foreskin fibroblast cultures. an inoculum of infected cells was up to 300-fold less sensitive than a cell-free inoculum of equivalent input multiplicity. a 10-fold increase in the dose of infectious units of either type of inoculum was associated with a 10-fold or greater decrease in interferon sensitivity. several aspects of the virus-cell interaction were examined and parameters indicative of cell infection w ... | 1978 | 202571 |
| inhibition of a complete replication cycle of human cytomegalovirus in actinomycin pre-treated cells. | the study of human cytomegalovirus (hcmv) in cultures of human embryo lung fibroblasts, pre-treated with actinomycin d, has shown that under these conditions the virus infection does not proceed beyond the 'early' events of the virus replication cycle. in the same experimental conditions the growth of poliovirus type i, vaccinia virus and herpes simplex type i virus, was completely unaffected. these results suggest that the complete hcmv replication cycle requires some cellular function(s) betwe ... | 1979 | 217966 |
| chronically persistent infection with human cytomegalovirus in human lymphoblasts. | cells from a line of human lymphocytes originating from a leukemic patient were persistently infected with human cytomegalovirus. assays of infectious centers and fluorescent antigen staining indicated that 1%--10% of the cells were infected. it appears that persistent infection is due to an equilibrium between the release of virus by infected host cells and the growth of uninfected cells rather than to defective virus or temperature-sensitive mutants. | 1979 | 220333 |
| induction of host dna synthesis and dna polymerase by dna-negative temperature-sensitive mutants of human cytomegalovirus. | 1979 | 220788 | |
| characterization of the human cytomegalovirus-induced chromatin factor responsible for activation of host cell chromatin template. | 1979 | 224579 | |
| size of infectious dna from human and murine cytomegaloviruses. | viral dna was isolated from human and murine cytomegalovirus by equilibrium centrifugation in cesium chloride gradients. the size of the dna was measured relative to t4 dna by velocity sedimentation in neutral glycerol gradients, and fractions were assayed for infectious dna. infectious murine cytomegalovirus dna sedimented as a single peak with an estimated molecular weight of 136 x 10(6). infectious human cytomegalovirus dna was detected in two peaks with molecular weights of 130 x 10(6) and 1 ... | 1979 | 225527 |
| production of plasminogen activator by human and hamster cells infected with human cytomegalovirus. | plasminogen activator was produced by both human embryo fibroblasts (a permissive system) and hamster embryo fibroblasts (a nonpermissive system) after exposure to human cytomegalovirus. the level of this activator was measured by using plates coated with [125i]fibrin. the production of plasminogen activator was enhanced when the human cells were exposed to human cytomegalovirus previously irradiated with uv light (5,520 to 55,200 ergs/mm2). | 1979 | 225562 |
| inhibition of cytomegalovirus-stimulated human cell ornithine decarboxylase by alpha-difluoromethylornithine. | 1. the relationship between synthesis of putrescine, human cytomegalovirus dna synthesis, cell dna synthesis, and human cytomegalovirus replication has been studied. 2. stimulation of ornithine decarboxylase activity by shifting low serum-arrested whole human embryo cells to high serum medium is inhibited more than 99% by 2.5 mm dl-alpha-difluoromethylornithine. the addition of dl-alpha-difluoromethylornithine to human cells arrested in low serum and subsequently stimulated by the addition of fr ... | 1979 | 227464 |
| a vesicular stomatitis virus (cytomegalovirus) pseudotype and its use in neutralization tests. | infection with vesicular stomatitis virus (vsv) of human diploid cells preinfected with the ad-169 strain of human cytomegalovirus (cmv) resulted in the formation of a vsv (cmv) pseudotype. its formation was favored by increasing the bicarbonate content in doubly-infected cultures. the pseudotype was capable of infecting not only human but also rabbit cells. pseudotype particles formed after infection with the tl 17 mutant of vsv, which carries a thermolabile lesion in its neutralization antigen ... | 1979 | 227814 |
| dna of human cytomegalovirus: size heterogeneity and defectiveness resulting from serial undiluted passage. | the majority of dna molecules associated with plaque-purified, low-multiplicity-passaged human cytomegalovirus (towne strain) had a molecular weight of approximately 150 x 10(6) and a molecular complexity of approximately 140 x 10(6). serial high-multiplicity passage resulted in the production of defective cytomegalovirions. an accumulation of smaller dna molecules packaged into virions was directly correlated with a decrease in infectivity and an increase in the particle-to-pfu ratio. the major ... | 1979 | 228055 |
| inactivation of human cytomegalovirus by the chemical carcinogen 4-nitroquinoline 1-oxide. | the infectivity of cytomegalovirus (cmv), strain davis, was inactivated by 4-nitroquinoline 1-oxide (nqo). a series of survival curves indicates that the rate of inactivation was directly dependent on the concentration of nqo over a range of 5 to 200 microgram/ml. at concentrations of i microgram/ml or less, inactivation of virus stock was not observed and at concentrations in excess of 200 microgram/ml, the cellular toxicity of residual nqo prevented quantification of the relatively low survivi ... | 1979 | 230304 |
| age-related neurocytotropism of mouse cytomegalovirus in explanted trigeminal ganglions. | human cytomegalovirus (cmv) causes severe congenital neurologic disease; adult neural infection is associated with transient cranial nerve palsies. in our experimental model, mouse cmv (mcmv) infects neurons and schwann and satellite cells of cranial nerve ganglions. to study the fate of mcmv in nerve tissue, we explanted trigeminal ganglions from newborn, suckling, and weanling mice, 3 to 36 days after intracranial inoculation. negative explants were co-cultivated with mouse embryo tissue cultu ... | 1979 | 230739 |
| enzyme-linked protein a: an enzyme-linked immunosorbent assay reagent for detection of human immunoglobulin g and virus-specific antibody. | a general-purpose reagent capable of reacting with immunoglobulin g in a modified enzyme-linked immunosorbent assay technique was prepared by using protein a coupled with horseradish peroxidase. the reagent detected low levels (0.003 to 1.0 microgram/ml) of human immunoglobulin g and was also applied in an enzyme-linked immunosorbent assay for titration of antibody to human cytomegalovirus. the antibody titers to human cytomegalovirus determined by enzyme-linked immunosorbent assay and by comple ... | 1979 | 231048 |
| comparative study of herpes group virus-induced dna polymerases. | a comparative biochemical study of virus-induced dna polymerases was made among the herpes group viruses: namely, herpes simplex virus (hsv) type 1 and type 2, human cytomegalovirus (hcmv) and varicella-zoster virus (vzv). although these virus-induced enzymes shared some biochemical properties, they differed in several important aspects. all these virus-induced dna polymerases could efficiently use poly(dc) . oligo(dg)12--18 and poly(da) . oligo(dt)12--18 as template-primers. however, in phospho ... | 1979 | 231586 |
| progressive neurologic disease caused by human cytomegalovirus. | 1979 | 233327 | |
| physical properties of cytomegalorvirus immune complexes prepared with igg neutralizing antibody, anti-igg, and complement. | human cytomegalovirus (cmv) strain ad 169 was reacted with igg antibody (ab) from a cmv-infected renal transplant patient. a portion of he virus was neutralized, but infectious cmv-ab complexes that could be neutralized by adding rabbit anti-human igg (a-igg) or complement (c) were also generated. the immune complexes were examined, and the following observations were made: 1) cmv ab sufficient to cause 94% neutralization did not induce measurable changes in virion size or density. 2) the cmv-ab ... | 1980 | 6243148 |
| effect of uv-irradiated human cytomegalovirus infection on exogenous thymidine metabolism in human embryonic lung cells. variation depending cells. | 1980 | 6247625 | |
| ultrastructural development and persistence of guinea pig cytomegalovirus in duet cells of guinea pig submaxillary gland. | salivary glands from hartley guinea pigs were experimentally infected with guinea pig cytomegalovirus (gpcmv) and examined by light and electron microscopy at different time intervals. characteristic intranuclear and intracytoplasmic viral inclusions were observed in duct cells of infected animals. viral inclusion counts and infectivity titers in the salivary gland reached maximum levels by 3 to 4 weeks after infection; infectivity persisted, though at reduced levels, for at least 30 weeks. elec ... | 1980 | 6247997 |
| lysosomal acridine orange uptake in fibroblasts transformed by sv40 or human cytomegalovirus. | lysosomes of living human fibroblasts, sv40-transformed rat fibroblasts and human cmv-transformed hamster fibroblasts were examined by fluorescence microscopy after pretreatment with acridine orange at a supravital concentrations (5 x 10(6) m). dye uptake by human primary fibroblast lysosomes was considerable and independent of the age of the cultures. in the transformed cultures, cytoplasmic granular red fluorescence indicating lysosomal acridine orange uptake could not be observed in part of t ... | 1980 | 6251701 |
| virus excretion and neutralizing antibody response in saliva in human cytomegalovirus infection. | the local secretory immune mechanism in infants with cytomegalovirus infection was studied by a measurement of neutralizing antibody in saliva. neutralizing antibodies were determined by the microculture plaque assay in 65 saliva specimens including 54 samples from cytomegalovirus-infected subjects and 11 from seronegative controls. in addition, cytomegalovirus isolation from saliva or urine or both and antibody determination in serum and saliva were simultaneously performed on seven infants wit ... | 1980 | 6253399 |
| immunofluorescence technique for detection of human cytomegalovirus (hcmv) antibodies against hcmv-induced late antigens with elimination of immunoglobulin g-receptor staining. | an immunofluorescence technique is described which permits the elimination of nonspecific cytoplasmic staining from late antigen preparations by using in situ a buffer containing nonidet p-40. | 1980 | 6253519 |
| seroconversion to virus-specific pre-early nuclear antigens in infants with primary cytomegalovirus infection. | antibodies to human cytomegalovirus (cmv)-specific antigens were determined in sera serially collected from 10 infants with primary cmv infection. antibodies to pre-early nuclear antigens (pena), which are detectable in human embryonic lung cells within 3 h of cmv infection by anticomplement immunofluorescence staining, developed in all the patients. however, in contrast to the early response of anti-early antigens (ea), anti-late antigens (la), and immunoglobulin m antimembrane antigens (ma), s ... | 1980 | 6254876 |
| [human cytomegalovirus and congestive cardiomyopathy (author's transl)]. | congestive cardiomyopathy in 3 cases, in which occurrence of human cytomegalovirus (cmv) infection was demonstrated, is described. in all cases significant rise in total cmv antibodies and in early antigen-antibodies was found: cmv was also isolated from urine and saliva in one, a 13 years old boy, and only from urine in the others two patients, 20 and 26 old men. immunoglobulin m was raised only in the first case, in which cmv infection was likely in act: instead in the others two cmv infection ... | 1980 | 6257578 |
| replication of human cytomegalovirus at supra-optimal temperatures is dependent on the virus strain, multiplicity of infection and phase of virus replication. | the kinetics of replication of five strains of human cytomegalovirus (cmv) were studied to determine the influence of (i) temperature, (ii) virus strain, (iii) m.o.i. and (iv) cell type. relative to growth at 37 degrees c (m.o.i. = 3 to 9) eclipse periods were extended from 24 to 48 h at 33 degrees c and to 72 h at 40.5 degrees c. yields were reduced at 33 degrees c and almost eliminated at 40.5 degrees c. no replication occurred in most instances at 40.5 degrees c and with 0.05 p.f.u./cell. tem ... | 1980 | 6257838 |
| [establishment of persistent cytomegalovirus infection in primary cultured trophoblastic cells (author's transl)]. | the primary cultured cells (ch) derived from chorionic villi were infected with human cytomegalovirus (cmv). the main results obtained are as follows. 1) the primary cultured trophoblastic cell (ch) infected with cmv have been maintained in the state of cmv persistent infection for over 6 months. these cells (ch/cmv) were the cmv-carrier cells in a balance between the cytolysis by cmv-specific cytopathic effect (cpe) and the growth of uninfected cells, showing the cmv persistent infection at the ... | 1980 | 6259876 |
| acute cytomegalovirus infections in leukemic mice. | mice infected with 2 x 10(3) plaque-forming units of mouse cytomegalovirus (mcmv) 3 days after receiving 300 to 400 spleen focus-forming units of friend leukemia virus developed a more severe mcmv infection than did normal animals. increased severity was demonstrated by the increased amounts of mcmv recoverable from the salivary glands of leukemic mice 1 to 5 weeks postinfection. in addition, the difference in the number of virus isolations from the kidneys, spleens, livers, and lungs of animals ... | 1980 | 6260649 |
| human cytomegalovirus-induced inhibition of exogenous thymidine uptake into cell dna in hel cells stimulated to proliferate with serum. | in contrast with the previous findings by us and others, human cytomegalovirus (hcmv) infection caused inhibition of exogenous 3h-dthd uptake into cell dna in human embryonic lung (hel) cells which were stimulated to proliferate with serum at the time of infection. the response of hel cells to hcmv infection varied depending on the hel cell population, and the inhibition of 3h-dthd incorporation was observed in a particular hel cell population prepared in our laboratory. that the inhibition was ... | 1980 | 6261422 |
| detection of cytomegalovirus genomes in human skin fibroblasts by dna hybridization. | a previous isolation of a human cytomegalovirus (cmv) from fibroblasts derived from intact skin of a charcot-marie-tooth disease patient has prompted examination of six blind-coded cultured human skin lines by cmv dna hybridization. the detection of cmv genome equivalents in three of the lines suggests that, in some cases, intact human skin may be a site of cmv latency. | 1980 | 6262444 |
| determination of iga antibodies to human cytomegalovirus by enzyme-linked immunosorbent assay (elisa). | a sensitive enzyme-linked immunosorbent assay (elisa) is described for detection of iga antibodies cytomegalovirus (cmv). the antigen consisted of a sonicated extract of cmv infected human embryo cells. the tested sera were absorbed with staphylococcus aureus (strain cowan 1) before analysis. rabbit antihuman iga peroxidase conjugate was used to detect human iga bound to viral antigen. in parallel, igm and igg antibodies to cmv were studied by elisa and by the immunoperoxidase antibody to membra ... | 1980 | 6262458 |
| estimation of antibodies to human cytomegalovirus by immunofluorescence and radioimmunoassay. | the 125j labeled igg fraction against rabbit igg of goat origin was employed for the detection of cmv igg and igm antibodies in the double indirect radioimmunoassay. the results were compared with those obtained in complement fixation, indirect immunofluorescence and anti-complement immunofluorescence tests. the application of labeled anti-fc antisera, instead of antisera against whole igg in the tests for detection of specific cmv igg antibody resulted in increased sensitivity of radioimmunoass ... | 1980 | 19852114 |
| igg-fc-binding receptors in cells abortively infected, or transformed, by human cytomegalovirus. | abortive human cytomegalovirus (hcmv) infection of normal hamster cells is accompanied by formation of cytoplasmic and surface receptors for the fc portion of nonimmune igg molecules. the process requires rna and protein synthesis but no dna synthesis. the surface receptors are detectable only transiently, in a small proportion of the cells. the receptors are also present in cells transformed by hcmv in vitro (87-trh-5 and cx-90-3b cell lines) as well as in cells of lines tsc-1 and tsc-2 from tu ... | 1981 | 6266208 |
| latency in vitro using irradiated herpes simplex virus. | human embryonic fibroblasts infected with u.v.-irradiated herpes simplex virus type 2 (hsv-2, strain 186) and maintained at 40.5 degrees c did not yield detectable virus. virus synthesis was induced by temperature shift-down to 36.5 degrees c. the induced virus grew very poorly and was inactivated very rapidly at 40.5 degrees c. non-irradiated virus failed to establish latency at 40.5 degrees c in infected cells. enhanced reactivation of hsv-2 was observed when latently infected cultures were su ... | 1981 | 6267167 |
| human cytomegalovirus dna: restriction enzyme cleavage maps and map locations for immediate-early, early, and late rnas. | 1981 | 6269285 | |
| cloning of the human cytomegalovirus genome as endonuclease xbai fragments. | restriction enzyme xbai dna fragments that represent 99% of the sequences from the long and short unique as well as the repeat sequences of the human cytomegalovirus (cmv) genome have been cloned into bacterial plasmid pacyc184. the viral dna sequences associated with the recombinant plasmids were analyzed by restriction mapping and by hybridization to fragments of authentic viral dna. the relationship of the cloned viral dna fragments to the xbai physical map of the viral genome is demonstrated ... | 1981 | 6282691 |
| effects of canavanine treatment on herpesvirus morphogenesis in cultured cells. | l-canavanine, a naturally occurring analog of arginine, effectively inhibited the morphogenesis of herpes simplex virus (hsv) and human cytomegalovirus (hcmv) when added at the time of infection, but allowed the expression of several cytopathic changes. exposure to canavanine at progressively later times ultimately led to qualitatively normal virion maturation. under no conditions were morphologically aberrant viral particles observed. however, hsv-infected cells treated at 3 h postinfection or ... | 1981 | 6282774 |
| detection of specific iga antibodies in serum of patients with varicella and zoster infections. | a sensitive solid-phase, enzyme-linked immunosorbent assay (elisa) was developed for detection of serum iga antibodies to varicella-zoster virus (vzv). the antigen consisted of a sonically disrupted extract of vzv-infected human embryo cells. rabbit antihuman iga peroxidase conjugate was used to detect human iga bound to viral antigens. in parallel, igm and igg antibodies to vzv were studied by elisa and by an immunoperoxidase antibody to membrane antigen technique, respectively. vzv iga antibod ... | 1981 | 7298297 |
| humoral and cellular immune responses of rabbits to sub-viral fractions of human cytomegalovirus. | we have raised specific sera against human cmv (strain ad 169) and have tested the immunogenicity of viral envelopes, removed using the non-ionic surfactant triton x-100. glycine-buffered extracts of infected mrc-5 cells elicited virus-neutralizing antibody to a titre of 1/1600. incubation of this material also elicited a considerable reaction against host cell antigens. inoculation of viral envelope material in freund's complete adjuvant also elicited virus-neutralizing antibodies. specific in ... | 1982 | 6299845 |
| [electron microscopic studies of human cytomegalovirus.--characterization of intracellular forms of parental viral dna molecules before and after onset of the viral dna synthesis]. | 1982 | 6296244 | |
| prevalence of antibodies to human cytomegalovirus in urban, kibbutz, and bedouin children in southern israel. | prevalence of antibody to human cytomegalovirus (cmv) was determined in a sample of 860 healthy children aged 1-13 years in beer sheva, in the negev region of israel. three groups of children were tested: (1) urban jewish children of low, middle, and high socioeconomic levels; (2) 8 rural communes (kibbutz) in which children live in close contact with each other from the 6th week of life under good hygienic conditions and a high standard of living; (3) bedouin, seminomadic arabs living in relati ... | 1982 | 6296312 |
| analysis of human cytomegalovirus nucleoprotein complexes. | when chromatin was isolated from cells infected with human cytomegalovirus, the virus dna remained with the chromatin fraction. if deproteinized virus dna was added to either isolated nuclei or chromatin, the dna was lost during the chromatin isolation. when isolated chromatin from cytomegalovirus-infected cells was banded in isopycnic metrizamide gradients, a single peak with a density of 1.18 g/cm3 was present. analysis of this peak in isopycnic neutral cscl gradients indicated that it contain ... | 1982 | 6283123 |
| inhibitors of polyamine biosynthesis block human cytomegalovirus replication. | 1982 | 6283366 | |
| a modified immunofluorescent test for detection of antibody against human cytomegalovirus. | 1982 | 6285116 | |
| effect of 9-(2-hydroxyethoxymethyl)guanine on viral-specific polypeptide synthesis in human cytomegalovirus-infected cells. | plaque-forming assay resulted in a 50 percent inhibitory dose by 9-(2-hydroxyethoxymethyl)guanine (acyclovir) against towne strain human cytomegalovirus (hcmv) of approximately 98 mumol. at the drug concentration of 200 mumol, we did not detect any significant inhibition of viral dna synthesis by crna-dna hybridization. however, at this drug concentration, the synthesis of at least two viral-specific late polypeptides (150k and 67k) was significantly retarded up to 48 hours after infection, but ... | 1982 | 6285739 |
| [human cytomegalovirus and disease (author's transl)]. | 1982 | 6286060 | |
| transformation of nih 3t3 cells with cloned fragments of human cytomegalovirus strain ad169. | nih 3t3 cells were transfected with restriction endonuclease and cloned human cytomegalovirus dna fragments to identify the transforming region(s). cleavage of human cytomegalovirus strain ad169 dna with xbai and hindiii left a transforming region intact whereas ecori inactivated this function. transfection of cells with cosmids containing human cytomegalovirus dna spanning the entire genome resulted in transformation by one cosmid, pcm1058, with the ad169 hindiii dna fragments e, r, t, and a'. ... | 1982 | 6287019 |
| human cytomegalovirus cycle in synchronized cells: electron microscope study. | human cytomegalovirus (hcmv) was inoculated in synchronized human fibroblasts at different phases of the cell cycle. the virus replication appeared strongly dependent on the host metabolic state, being faster when the infection was carried out in g2 and s phase. | 1982 | 6287176 |
| lack of detectable dna damage in cells productively infected with human cytomegalovirus. | 1982 | 6289055 | |
| human cytomegalovirus dna: bamhi, ecori and psti restriction endonuclease cleavage maps. | the cloned hindiii fragments of human cytomegalovirus (hcmv) strain ad169 dna were mapped with respect to the bamhi, ecori and psti restriction endonuclease cleavage sites. composite restriction endonuclease cleavage maps for the entire virus genome were constructed using the previously established linkages between the hindiii fragments. | 1982 | 6290339 |
| neutralization and sensitization of cytomegalovirus by igg antibody, anti-igg antibody, and complement. | human cytomegalovirus (cmv) strain ad 169 was reacted with igg antibody obtained from infected renal transplant patients, and the degree of neutralization was determined. the mixture of antibody and virus was then incubated with anti-human igg (a-igg) or complement (c) to measure additional neutralization by these agents and thus to estimate the concentration of infectious virus-antibody complexes which had been formed. neutralization and formation of infectious complexes susceptible to neutrali ... | 1982 | 6292359 |
| nuclear and cytoplasmic inclusions in disseminated human cytomegalovirus infection. | a case of disseminated human cytomegalovirus infection is described in a full-term female who expired 3 1/2 h after birth. cytomegalic inclusions, both intranuclear and intracytoplasmic, were observed mainly in the kidneys, liver, lungs, and anterior pituitary but were not seen in the bone marrow and spleen. whereas the nuclear inclusions consisted of an amorphous filamentous meshwork with a variable number of pleomorphic capsids, the cytoplasmic inclusions were composed of membrane-bound aggreg ... | 1982 | 6293134 |
| ultraviolet irradiation of murine cytomegalovirus. | ultraviolet irradiation of murine cytomegalovirus (mcmv) caused a rapid dose-related decline in virus infectivity, manifested by virus antigen induction, and in virus production as measured by plaque formation and infectious centre assay. the virus survival curve was multi-component, suggesting host cell-assisted reactivation. multiplicity reactivation and photoreactivation of mcmv were not observed in these experiments. productive infection was more sensitive to u.v. irradiation than was virus ... | 1982 | 6294231 |
| cell-mediated immunity in human cytomegalovirus infection. | the direct leukocyte migration inhibition test, in response to cytomegalovirus stimulation, was used to study cell-mediated immunity in a group of children with cytomegalovirus infection. the test was impaired in children with chronic disease associated with cytomegaloviruria. in those cases with no viruria at the moment of the test, leukocyte migration inhibition was normal. our data suggest that the acquired chronic cytomegalovirus infection may be sustained by a state of specific cellular des ... | 1982 | 6279516 |
| use of recombinant plasmids to investigate the structure of the human cytomegalovirus genome. | human cytomegalovirus (hcmv) dna was digested with restriction endonucleases and the fragments characterized with respect to molecular weight and relative mole proportions. the terminal fragments were identified by digesting hcmv dna with exonucleases before restriction endonuclease treatment and subsequent gel analysis. the hindiii fragments of hcmv dna were cloned in escherichia coli and recombinant plasmids were characterized by digestion with restriction endonucleases and by molecular hybrid ... | 1982 | 6279770 |
| detection of human cytomegalovirus- specific iga antibodies in colostrum by enzyme-linked immunosorbent assay (elisa). | fifty women were examined after delivery for the prevalence of antibodies to human cytomegalovirus in colostrum and sera. eighty percent of them had specific cmv igg antibodies in the sera, as determined by the immunoperoxidase antibody to membrane antigen (ipama) technique. of the cmv-seropositive women, 60% had specific cmv iga antibodies in high titer in the colostrum as determined by enzyme-linked immunosorbent assay (elisa). in only two of the seropositive women were specific cmv iga antibo ... | 1982 | 6279779 |
| comparison of the occurrence of antibodies to human cytomegalovirus as demonstrated by complement fixation, enzyme-linked-immunosorbent assay and indirect immunofluorescence. | 180 serum specimens from pregnant women and renal transplant recipients, were studied for the presence of antibodies to human cytomegalovirus (hcmv) by complement fixation (cf), enzyme-linked-immunosorbent assay (eia) for igg and igm, and indirect immunofluorescence (iif). iif was carried out separately against immediate-early, early and late hcmv-induced antigens. a positive cf test was always associated with the presence of antibodies against late virus antigens. eia procedures were able to de ... | 1982 | 6100235 |
| effects of acyclovir combined with other antiviral agents on human cytomegalovirus. | at present, there is no effective therapy for human cytomegalovirus (cmv) infections. although acyclovir inhibits in vitro clinical isolates of cmv, preliminary human trials suggest that acyclovir may not be successful as a single antiviral agent in treating cmv infections. the anticmv activity of acyclovir in combination with human fibroblast interferon (ifn-beta), phosphonoformic acid (pfa), or trifluorothymidine (tft) was therefore evaluated. when acyclovir (20 microm) was combined with ifn-b ... | 1982 | 6179416 |
| high efficiency latency and activation of herpes simplex virus in human cells. | herpes simplex virus (hsv) exists in humans in a latent form that can be activated. to characterize the molecular basis of the cell-virus interactions and to analyze the state of the latent hsv genome, an in vitro model system was established. in this system a large fraction of the latently infected cells contain an hsv genome that can be activated. cell survival was reduced minimally after repression of high multiplicity hsv type 1 (hsv-1) infection of human fibroblast cells with (e)-5-(2-bromo ... | 1982 | 6180477 |
| cytomegalovirus antigen within human arterial smooth muscle cells. | arterial tissues from carotid artery plaques or from punch-biopsy samples of uninvolved areas of the aorta were removed from 132 patients with atherosclerosis during blood-vessel surgery. cells morphologically identical to smooth muscle cells were cultured from 26 to 126 plaque samples and from 6 of 6 punch-biopsy samples. immunofluorescence tests of these cells showed that more than 25% of the cell cultures from both types of sample contained antigens of human cytomegalovirus (cmv) but not of h ... | 1983 | 6136795 |
| search for dna sequences of human cytomegalovirus in kaposi's sarcoma tissues with cloned probes. preliminary report. | 1983 | 6087726 |