Publications

an agent from e. coli causing hemorrhage and regression of an experimental mouse tumor. v. behavior of the agent in the presence of various reagents.		1954	13233855
the therapeutic activity of gamma globulin; chloramphenicol combinations in mouse infections due to salmonella, pneumococci, escherichia coli, or pasteurella.		1957	13521858
in vitro studies on the interaction between mouse peritoneal macrophages and strains of salmonella and escherichia coli.	virulent strains of salmonella opsonized with normal mouse plasma are never phagocytosed as well as avirulent strains. the virulent strains of salmonella phagocytosed after opsonization with normal mouse plasma are able to multiply within normal mouse peritoneal macrophages, whereas under similar experimental conditions the avirulent strains are killed. when virulent strains of salmonella are opsonized with specific antiserum or plasma from bcg-infected mice, they are treated by normal mouse mac ...	1960	13789820
[studies on experimental shigella infections. 3. on the transferability of drug resistance from a resistant strain of shigella to a sensitive strain of escherichia coli in the mouse].		1962	13957111
[drug-resistance transmission of enteric bacteria in the intestines of the germ-free mouse and the normal mouse].		1962	13958319
experimental pyelonephritis in the mouse produced by escherichia coli, pseudomonas aeruginosa and proteus mirabilis.		1964	14106358
inhibition of the growth of implanted mouse carcinoma by an irradiated bacterial culture.		1964	14152838
specificity of mouse uridine phosphorylase. chromatography, purification, and properties.		1964	14154460
increased susceptibility of mice infected with mouse adenovirus to escherichia coli-induced pyelonephritis.	mouse adenovirus causes a persistent infection in the mouse kidney that produces extensive mononuclear cell infiltrates in cortex and medulla. tubular necrosis, dilatation, and occasional collapse occur but no glomerular changes or periglomerular fibrosis have been observed. acute and chronic adenovirus infection of the kidney predispose the kidney to develop acute pyelonephritis when the mouse is challenged by the intravenous or retrograde route with escherichia coli.	1964	14238929
cofactor-dependent aldose dehydrogenase of rhodopseudomonas spheroides.	niederpruem, donald j. (university of california, berkeley), and michael doudoroff. cofactor-dependent aldose dehydrogenase of rhodopseudomonas spheroides. j. bacteriol. 89:697-705. 1965.-particulate enzyme preparations of cell extracts of rhodopseudomonas spheroides possess constitutive dehydrogenase and oxidase activities for aldose sugars, reduced nicotinamide adenine dinucleotide (nadh(2)), and succinate. the dehydrogenation of aldoses requires an unidentified cofactor which is not required ...	1965	14273648
attempts to detect an integrated polyoma genome by nucleic acid hybridization ii. complementarity between polyoma virus dna and normal mouse synthetic rna.		1965	4285375
mouse c-reactive protein and endotoxin-induced resistance.	patterson, l. t. (university of texas, galveston), and r. d. higginbotham. mouse c-reactive protein and endotoxin-induced resistance. j. bacteriol. 90:1520-1524. 1965.-the relationship between the level of c-reactive protein (crp) in the sera of mice and resistance to staphylococcus aureus infection after the injection of escherichia coli endotoxin was studied. the crp level was essentially unchanged at 6 hr after endotoxin, and resistance was slightly decreased. at 24 hr after endotoxin, both c ...	1965	5322718
delivery and multiplication of bacteria in mouse brain: effect of carbon dioxide.		1966	5333258
regression of mouse ascites tumors by the treatment with bacterial extracts.		1966	5338893
possible extracellular and intracellular bactericidal actions of mouse complement.		1967	5342410
the specificity of inhibition of l1210 growth by mouse thymus deoxyribonucleic acid.		1967	5335874
techniques used in the establishment and maintenance of a barrier mouse breeding colony.		1968	4247420
behavior of the no-beta-d-glucosiduronic acid of n-acetyl-n-phenylhydroxylamine as a substrate for beta-glucuronidase.	1. biosynthetic sodium (n-acetyl-n-phenylhydroxylamine no-beta-d-glucosid)-uronate is hydrolysed completely by purified mouse urinary beta-glucuronidase into the products n-acetyl-n-phenylhydroxylamine and glucuronic acid. the hydrolysis is inhibited by saccharo-(1-->4)-lactone. these results not only confirm the identity and purity of the substrate but also establish it as a substrate for beta-glucuronidase. 2. mammalian and bacterial beta-glucuronidase preparations hydrolysed the substrate at ...	1968	4866429
the effect of exogenous ribonucleic acid on alkaline phosphatase activity in the ovarectomized mouse uterus.		1968	4866501
asparagine synthetase in asparaginase resistant and susceptible mouse lymphomas.		1968	4869945
preliminary report on the production of experimental mastitis in the mouse.		1969	4895981
small intestinal mucosal cell proliferation and bacterial flora in the conventionalization of the germfree mouse.	the relationship between intestinal colonization and the small bowel mucosal cellular proliferation rate during conventionalization of the germfree mouse was examined. 16 mice were maintained under standard germfree conditions, and 54 others were conventionalized. migration of the small bowel epithelial cells was followed by radioautography with administration of tritiated thymidine. colonization was followed by qualitative and quantitative bacteriological fecal analyses. the percentages of the ...	1969	4896909
[study of the penetration of a heterologous deoxyribonucleic acid in mouse fibroblasts in vitro].		1969	4979276
experimental approaches to homogenous antibody populations. mouse iga myeloma proteins that bind polysaccharide antigens of enterobacterial origin.		1970	4983816
critical influence of an enzyme-elevating virus upon long-term remissions of mouse leukemia following asparaginase therapy.		1970	4949171
light-induced cross-linking of dna in the presence of a furocoumarin (psoralen). studies with phage lambda, escherichia coli, and mouse leukemia cells.		1970	4927248
[study of mouse or rat livers perfused "in vitro" with blood containing bacterial dna].		1970	4098015
studies on the influence of host defence mechanisms on the antimicrobial effect of chemotherapeutic agents. effect of antibiotics on phagocytosis of mouse-peritoneal-macrophages in vitro.		1971	4326907
lesions in escherichia coli cell walls caused by the action of mouse complement.		1971	4928548
[effect of single structural components of bacterial lipopolysaccharides on radiosensitivity of the mouse].		1971	4933960
the reversible inhibition of catalase activity by nucleotides and its possible relationship to mouse liver catalase depression induced by biological substances.		1971	4399192
uridinediphosphogalactose-4-epimerase deficiency in salmonella typhimurium and its correction by plasmoid-borne galactose genes of escherichia coli k-12: effects on mouse virulence, phagocytosis, and serum sensitivity.	the synthesis of smooth lipopolysaccharide (lps) in relation to mouse virulence and resistance to serum bactericidal activity in vitro and to rapid intravenous clearance in vivo was studied in salmonella typhimurium by using a virulent [median lethal dose (ld(50)) = 10(2)], smooth, and genetically marked strain, a uridinediphosphogalactose epimerase-deficient mutant of it which was, therefore, rough, and a derivative of the mutant made smooth again by acquisition of the galactose-positive genes ...	1971	4949486
enteropathogenicity of escherichia coli. i. evaluation of mouse intestinal loops.	a ligated mouse intestinal loop assay has been introduced to evaluate the enteropathogenicity of strains of escherichia coli. when inoculated as whole cultures, 19 (34%) of 55 strains isolated from diarrheic infants, each of 4 strains associated with diarrhea in human adults, and 5 of 7 strains associated with porcine coli bacillosis were found capable of causing fluid accumulation in this model. in contrast, only 1 of 28 strains isolated from normal individuals was positive in this test. steril ...	1971	4949503
localization and biological and physicochemical properties of the cell wall lipopolysaccharide of rhodopseudomonas capsulata.	electron micrographs of phenol-water-extracted lipopolysaccharide (lps) of rhodopseudomonas capsulata show filamentous and netlike aggregates. treatment of the lps with sodium deoxycholate resulted in a reversible splitting into subunits. the lps represents a cell wall constituent with o-antigenic specificity. in passive hemagglutination tests, high titers were obtained when erythrocytes sensitized with untreated or heat-treated lps were incubated with antisera obtained by immunization of rabbit ...	1972	5018024
function of various intestinal bacteria in converting germfree mice to the normal state.	earlier work had shown that a collection of anaerobic bacteria, in conjunction with facultative anaerobes, may be implanted into germfree mice, thereby rendering the animals "normal" with respect to a variety of parameters tested. the present experiments indicate that a different collection of anaerobic bacteria, isolated from the cecum of normal mice, was necessary to convert germfree mice to the "normal" state when the animals were fed a crude diet, rather than the refined food which had been ...	1972	4631910
failure of poly (i-c) to enhance killing of escherichia coli by mouse peritoneal macrophages.		1972	4561487
characterization of a mouse ascites cell-free system.		1972	4565029
characteristics and products of a cell-free polypeptide synthesizing system from neonatal and adult mouse brain.		1972	4569109
the distribution and binding of tritiated polymyxin b in the mouse.		1972	4343416
antigenic relationships in mammalian dna polymerase.	rabbit antibody was prepared against a high-molecular-weight dna polymerase purified from the soluble fraction of calf thymus gland. this antibody does not inhibit terminal deoxynucleotidyl transferase isolated from that source, but does inhibit both low-molecular-weight and high-molecular-weight dna polymerases isolated from cytoplasmic and nuclear fractions of a number of mammalian tissues (mouse l cells, calf thymus, phytohemagglutinin-stimulated human lymphocytes, rat liver, and rabbit bone ...	1972	4110484
evidence for single copies of globin genes in the mouse genome.		1972	4117010
the production of vesicular stomatitis virus by antigen- or mitogen-stimulated lymphocytes and continuous lymphoblastoid lines.	a variety of lymphoid cell populations were examined in terms of their ability to replicate vesicular stomatitis virus (vsv), a lytic, rna-containing virus maturing at the cell surface. the number of cells capable of producing vsv was estimated in terms of infectious centers by the virus plaque assay (vpa), and morphologically by electron microscopy (em). the lymphoid cells examined in this study included: (a) lymph node cells from delayed hypersensitive guinea pigs stimulated by specific antige ...	1973	4348276
comparison of the acceptance activity of the ribosome-bound and the total cellular transfer ribonucleic acids from sv40-transformed mouse fibroblasts.		1973	4360078
[persistance of morphologic differences between thymodependent and thymo-independant lymphocytes in the mouse after selective antigenic sollicitation of one or the other population].		1973	4214368
transfer ribonucleic acids from mouse plasmacytoma tumors producing kappa and immunoglobulin chains.		1973	4569775
age-dependent changes in sensitivity to antigen in the mouse.		1973	4576505
common enterobacterial antigen. ii. effect of immunization on challenge with heterologous bacilli.	studies were carried out evaluating the protective activity of immunization with common enterobacterial antigen (ca) against challenge with heterologous gram-negative bacilli. active immunization of mice with escherichia coli 0:14 elicited titers of antibody to ca of 1:640 or greater but completely failed to enhance resistance to challenge by mouse virulent strains of klebsiella pneumoniae or e. coli. similarly, two lots, 324 and 422, of rabbit antisera to ca failed to afford passive protection ...	1973	4576679
regulation of immunoglobulin synthesis by dextran.	dextran, of the variety commonly used as plasma expander, markedly altered antibody synthesis to an unrelated antigenic stimulus, srbc, in two animal species, guinea pig and mouse. the time at which dextran was administered relative to antigen was found to be most critical for increasing or decreasing the number of igm and igg pfc. furthermore, these times differed for the two species studied. typically, when given to guinea pigs 6 h before srbc, dextran caused a 20-fold rise in igm-producing ce ...	1973	4577618
protein synthesis initiation in eukaryotes. characterization of ribosomal factors from mouse fibroblasts.		1973	4581104
susceptibility of the mouse intestine to heat-stable enterotoxin produced by enteropathogenic escherichia coli of porcine origin.	ligated intestinal loops of mice were found suitable for the assay of heat-stable enterotoxin produced by enteropathogenic escherichia coli strains of porcine origin; loops inoculated with heat-labile enterotoxin failed to react.	1973	4584587
effect of 5-bromodeoxyuridine on chromatin transcription in confluent fibroblasts.	3t6 mouse fibroblasts were grown in 5-bromodeoxyuridine (brdu) so that approximately 20% of the thymine residues in dna were replaced by brdu. brdu replacement caused an alteration in the relative incorporation of labeled nucleotide precursors into rna. the rna synthesized by cells grown in brdu has a lower proportion of adenine and a higher guanine complement. this was shown for (a) nascent rna made in vivo by confluent monolayers of cells in culture; (b) rna synthesized in vitro on a chromatin ...	1974	4592691
biochemical properties of escherichia coli low-molecular-weight, heat-stable enterotoxin.	the low-molecular-weight, heat-stable type of escherichia coli enterotoxin (st) was obtained from sterile syncase broth filtrates of human and animal enteropathogenic e. coli strains. st was assayed in infant mice, and it was found that this assay was specific for st in that the high-molecular-weight, heat-labile type of e. coli exterotoxin would not cause fluid accumulation in these animals. st was first detected in the broth of a culture of human strain 334a(015:h11) 5 h after inoculation, imm ...	1974	4593342
influence of a metazoan infection in the mouse on enteric colonization and immune response to escherichia coli.		1974	4609298
a peroxidase inhibitor in leukemic akr mouse spleen cells.		1974	4609605
effect of normal mouse serum on mouse lymphocyte transformation in vitro.		1974	4277456
skin reaction produced by endotoxins and lactobacillus in mouse.		1974	4215912
selective activation of mouse t and b lymphocytes by periodate, galactose oxidase and soybean agglutinin.		1974	4372060
hybridization characteristics of enzymatically synthesised dna complementary to mouse immunoglobulin messenger rna.		1974	4136787
2'-o-methylated oligonucleotides in ribosomal 18s and 28s rna of a mouse hepatoma, mh 134.	simple two-dimensional thin-layer chromatography was found to be useful for the separation of sugar methylated dinucleotides in rna. of the 16 possible sequences of the type nm-np, 15 were separated and all the sequences were determined. in a mouse hepatoma, mh 134, the levels of the sugar methylation in the 18s and 28s rna molecules were 17-18 and 11-12 per 1000 nucleotides, respectively. thus, 18s rna contained approximately 35 2'-o-methylated dinucleotides and 28s rna approximately 60 2'-o-me ...	1975	164900
mapping of the sv40 specific sequences transcribed in vitro from chromatin of sv40 transformed cells.	chromatin was isolated from sv40 transformed mouse cells (sv3t3) and transcribed with escherichia coli rna polymerase. the sv40 specific transcripts were analyzed by annealing the rna to the minus strands of purified fragments of sv40 dna produced by cleavage of the dna with a restriction enzyme isolated from hemophilus aegyptius. quantitation of the frequency of transcription from the region (fragments a and d) was transcribed five to ten times more frequently than the remaining regions. these ...	1975	167804
temperature dependence of strand separation of the dna molecules containing integrated sv40 dna in transformed cells.	it has previously been demonstrated that the dna molecules containing a genetic marker from one region of a bacterial genome undergo complete strand separation at temperatures usually different from the molecules containing a genetic marker from another region of the genome. the experiments also showed that if a group of molecules undergo complete strand separation over a narrow temperature range, of the order of one to three degrees, it is highly likely that they all come from one region of the ...	1975	170596
orientation of the complementary strands of polyoma virus dna with respect to the dna physical map.	the chemical polarities of the two strands of polyoma virus dna with respect to the dna physical map have been determined by hybridization of restriction endonuclease fragments specifically labeled with [125i]dcmp at their 3' termini to asymmetric polyoma complementary rna (the product of in vitro transcription of viral dna by escherichia coli rna polymerase). the orientations of the polyoma-specific stable rna transcripts present in the cytoplasm of productively linfected mouse cells have been ...	1975	173885
urinary-tract infection: localisation and virulence of escherichia coli.	virulence of 15 strains of escherichia coli from the human upper urinary tract was compared with that of 16 strains from the lower urinary tract, using an ascending infection in the mouse. no significant difference was found. there was no significant difference in frequency of k antigen and ability to ferment dulcitol between 32 lower strains and 31 upper strains. however, 22 strains containing k antigen, regardless of anatomical site of localisation, were more significantly likely to cause infe ...	1975	46051
assay, characterization, and localization of an enterotoxin produced by salmonella.	an enterotoxic factor isolated from cultures of salmonella yielded reproducible results in the suckling mouse model in contrast to other animal models. the enterotoxin appears to possess properties similar to both the heat-stable and heat-labile enterotoxins of escherichia coli. preliminary results indicate that the toxin is a protein, is located in the cell wall or outer-membrane fraction, and is difficult to separate from other cell wall constituents.	1975	234910
excision-repair of 4-nitroquinolin-1-oxide damage responsible for killing, mutation, and cancer.	excision-repair of dna base damage produced by 4-nitroquinoline-1-oxide (4nqo) was compared in escherichia coli, human cells, and mouse cells. paper chromotography of acid hydrolysates of dna extracted from cells treated with 3h-labeled 4nqo revealed four peaks; two kinds of 4nqo-guanine adduct, one kind of 4nqo-adenine adduct, and free 4-aminoquinoline-1-oxide (4aqo). about 80% of the initially formed 4nqo-purine adducts were excised from dna in e. coli uvra+ cells during 60 min postincubation, ...	1975	811213
immune responses of specific pathogen-free and gnotobiotic mice to antigens of indigenous and nonindigenous microorganisms.	strains of indigenous escherichia coli, bacteroides, and lactobacillus were isolated from the gastrointestinal tracts of specific pathogen-free (spf) mice. nonvaccinated spf mice exhibited in their spleens low numbers of plaque-forming cells (pfc) and rosette-forming cells reacting with antigens of these andigenous bacteria. pfc reacting with these bacterial antigens were not detected in infant spf mice until 7 days after birth. compared with nonvaccinated controls, spf mice vaccinated parentera ...	1975	1089600
receptor sites for antigen-antibody complexes on cells derived from solid tumors: detection by means of antibody sensitized sheep erythrocytes labeled with technetium-99m.	surface receptor sites for the fc portion of antigen-antibody complexes were demonstrated on cells derived from three methylcholanthrene-induced fibrosarcomas, one of strain c3h and two of strain balb/c origin, two spontaneously occurring malignant melanomas (b16 in strain c57bl/6 and harding-passey in strain balb/c mice), a moloney sarcoma virus-induced tumor of strain balb/c origin and the walker 256 carcinosarcoma of holtzman rats. primary cell cultures derived from these tumors adsorbed tech ...	1975	1089728
effect of exposure to hyperoxic, hypobaric, and hyperbaric environments on concentrations of selected and aerobic and anaerobic fecal flora of mice.	alterations in selected aerobic and anaerobic fecal microflora of the mouse were determined during exposure to hyperoxic and normoxic hypo- and hyperbaric environments. examination of fecal cultures obtained during exposure for 6 weeks to either 60 or 77% oxygen concentration 1 atmosphere absolute revealed little alteration in the aerobic or anaerobic flora. there appeared to be only a retardation in the reduction of the klebsiella-enterobacter flora which normally occurs after weaning. during e ...	1975	1090255
comparative mitogenic responses of t-cells and b-cells in spleens of mice of varying age.	mouse spleen cell mitogenic responses to phytohemagglutinin (pha), conconavalin a (con a), staphylococcal enterotoxin b (seb) and lipopolysaccharide (lps) were studied as a function of age. in cd-1 mice, spleen cell dna synthesis in response to stimulation by pha, con a and seb varied from 18% - 33% of adult values for the first 3 weeks of life. by 6 weeks, the pha response was 63% of the adult response and achieved adult values by 8 weeks of life. in contrast to these t-cell responses, dna sple ...	1975	1090516
effects of concanavalin a on the in vitro responses of mouse spleen cells to t-dependent and t-independent antigens.	?the stimulatory and inhibitory effects of concanavalin a (con a) on the in vitro primary immune responses to a t-dependent antigen, sheep erythrocytes (srbc) and a t-independent antigen, tnp-lipopolysaccharide (tnp-lps) have been studied. inhibition of the response to both antigens was optimal when 2 mug con a were added at the initiation of the culture period. the response to srbc was considerably enhanced by the addition of con a 24 hr later. in contrast, this late addition did not stimulate ...	1975	1090654
effect of acinetobacter glutaminase-asparaginase treatment on free amino acids in mouse tissues.	acinetobacter glutaminase-asparaginase (aga) and escherichia coli asparaginase were compared for their effects on plasma and tissue levels of amino acids, ammonia, and glutamyl transferase activity in the mouse. free asparagine was depleted similarly in plasma and tissues by both enzymes. aga treatment produced partial depletion of glutamine concentrations in muscle, spleen, small intestine, and liver. brain and kidney glutamine concentrations actually rose with treatment. despite over 100-fold ...	1975	1091350
the primary structure of the major cytoplasmic valine trna of mouse myeloma cells.	this paper describes the derivation of the primary structure of the major valine trna in the cytoplasm of mouse myeloma cells. approximately 75% of the nucleotide sequence of this trna is also shared by the trna1-val of yeast, this homology serving as a further indication of the extreme conservation of the structures of the trnas of different eukaryotic organisms. a novel feature of mouse myeloma trna1-val is its loop iv sequence: -u-psi-c-g-m1a-a-a-. this particular loop iv sequence has not pre ...	1975	1091487
functional heterogeneity among the t-derived lymphocytes of the mouse. iii. helper and suppressor t-cells activated by concanavalin a.		1975	1095214
inhibition of thymidylate synthetase and dihydrofolate reductase by naturally occurring oligoglutamate derivatives of folic acid.	naturally occurring oligoglutamate derivatives of folic acid in extracts of escherichia coli have been isolated on the basis of their inhibitory actions toward thymidylate synthetase and dihydrofolate reductase. the inhibitor of thymidylate synthetase has been identified as n-5-formyl-h4pteroyloligoglutamate (approximately 5 amino acid residues). it is 150-fold more inhibitory than the monoglutamate. synthetic n-5-formyl derivatives containing 3 to 6 glutamyl residues were prepared and found to ...	1975	1095583
isolation and partial characterisation of the relaxation protein from nuclei of cultured mouse and human cells.	a protein, called relaxation protein because of its ability to remove superhelical turns in closed-circular dna, has been isolated and partially characterized from the nuclei of la9 mouse and hela cells. the purification was facilitated by an assay method, with pm2 dna, which used the fluorescence enhancement of the intercalating dye ethidium bromide upon binding to the closed-circular dna. the amount of dye bound depends upon the degree of the superhelix density of the dna. the relaxation produ ...	1975	1100383
studies of mouse mitochondrial dna in escherichia coli: structure and function of the eucaryotic-procaryotic chimeric plasmids.	the mouse mitochondrial dna genome has been cloned in escherichia coli by linking it to the psc101 plasmid replicon at cohesive-ended cleavage sites generated by eco rl restriction endonuclease. the four possible configurations of chimeric molecules that contain the nucleotide sequences of mitochondrial dna in their native relationship were distinguished by hind iii restriction endonuclease digestion and electron microscopic heteroduplex analysis. chimeric molecules utilize the psc101 replicatio ...	1975	1102112
inflammatory mediators in culture filtrates of escherichia coli.	escherichia coli, when cultured on a simple medium containing only glucose and inorganic compounds, release soluble factors which have a variety of biologic effects on cells in vitro. these low molecular weight (less than 12,000) substances are capable of: a) reversibly inhibiting the migration of macrophages, b) causing chemotactic attraction of neutrophils, c) inducing blast transformation of lymphocytes, and d) producing cytotoxic effects on mouse fibroblasts in culture. although these activi ...	1975	1103632
experimental amyloidosis: the inducer is a polyclonal b-cell activator to which susceptibility is under genetic control.	experimental amyloidosis in mice can be induced by repeated injections of casein. it has now been demonstrated that casein induces strong polyclonal antibody synthesis in mouse b spleen lymphocytes. this effect is much more pronounced in spleen cells from anyloid-susceptible mice (cba/j) than amyloid-resistant mice (a/j). it is suggested that amyloidosis can be due in some instances to a constant exposure for molecules which induce polyclonal b-cell activation.	1975	1104749
blastogenesis of human lymphocytes by endotoxin.	lipopolysaccharides of s. typhimurium, s. enteritidis and e. coli at wide range of concentrations were used to induce blastogenesis in human and mouse (nude) lymphocytes. human lymphocytes from peripheral blood showed positive response to at least one source of lps (stimulation index of 2-9). the optimum concentration resulting in maximum stimulation varied with different individual, sources and concentrations of lps used. lymphocytes from cord blood failed to respond to lps, but had positive re ...	1975	1107213
investigation of the effect of k antigen in escherichia coli urinary tract infections by use of a mouse model.	urinary infection could be produced in mice by the inoculation of escherichia coli into the bladder, provided that sutures had first been inserted into the bladder wall to act as foreign bodies. while the bladder was almost always infected, thekidneys were involved less often. the frequency of kidney infection was directly proportional to the k antigen of the infecting strain of e. coli. the significance of k antigens in relation to host defence mechanisms and tissue invasion is discussed.	1975	769819
salmonellosis in mice: immunization experiments with salmonella-escherichia coli hybrids.	mice vaccinated with escherichia coli hybrids experesing salmonella o antigens were protected against lethal challenge doses of salmonella strains. counts of challenge bacteria in mouse organs revealed that a live hybrid vaccine was slightly more efficient than an acetone-killed vaccine.	1976	773838
mouse spleen lymphocyte bactericidal and peroxidase activities: enhancement by whole body x-irradiation.	a 20,000 g pellet obtained from the homogenate of cd1 mouse spleen cells has measurable peroxidase and bactericidal activities. whole body x-irradiation stimulates the in vitro peroxidative and bactericidal activities of the spleen cells. these cells do not phagocytize. however, addition of polystyrene latex particles to spleen cells from non- or x-irradiated mice, increases glucose oxidation. the extent of this stimulation (three- to fourfold) is less than that of pmn (six- to tenfold). interac ...	1976	778856
influence of certain indigenous gastrointestinal microorganisms on duodenal alkaline phosphatase in mice.	alkaline phosphatase activity was assayed in duodenal homogenates or extracts from adult specific pathogen-free (spf) and germfree mice and gnotobiotic mice monoassociated with a lactobacillus sp., a bacteroides sp., or a coliform strain indigenous to spf mice. activity levels of the enzyme were much higher in the preparations from germfree mice than in those from the spf controls. in the gnotobiotes monoassociated either with a freshly isolated lactobacillus sp. or a bacteroides sp., the levels ...	1976	779647
activity of stringent protein in ribosomes of escherichia coli during the growth cycle.	to investigate whether the stringent protein in escherichia coli was lost from ribosomes at certain phase of growth as in other organisms (bacillus subtilis, mouse embryo), cells growing at various phases of the growth cycle were harvested and ribosomes tested for activity. the results showed that in e. coli, stringent protein was associated with the ribosomes throughout the growth cycle. the peak activity for the synthesis of guanosine tetra- and pentaphosphates appeared around midlogarithmic p ...	1976	779841
bacterial endotoxin and impaired fetal development.	small doses of e. coli endotoxin given to pregnant mice on the 13th day of pregnancy caused only a mild maternal illness but induced resorption of approximately half the number of fetuses in each mouse. the remaining live fetuses developed normally and showed no evidence of retarded growth or malformations. the weights of their placentas and maternal spleens increased significantly. endotoxin given on the 6th day of pregnancy caused a small reduction in fetal weights.	1976	780127
the structures and fidelity of replication of mouse mitochondrial dna-psc 101 ecori recombinant plasmids grown in e. coli k12.	recombinant dnas containing the e. coli plasmid psc101 and mouse cell (la9) mitochondrial dna (mtdna) were formed in vitro via ligation of dna fragments from limit ecori endonuclease digests and were used to transform e. coli k12. four structurally different recombinant plasmid dnas from transformed clones were characterized. two of these were analyzed extensively and the mtdna portions compared with mtdna from la9 cells. no differences were detected in the physical or chemical properties examin ...	1976	782720
antigenicity testing of inactivated oral enteric bacterial vaccines for human use.	this study deals with the laboratory testing of the antigenicity of inactivated oral enteric bacterial vaccines for human use, viz. typhoid, dysentery, escherichia coli and cholera vaccines. for the assays the following methods are applied : 1) various types of active mouse protection tests 2) serum and coproantibody determinations in immunized persons and experimental animals, 3) protective ability of the immune sera in mice and embryonated eggs, 4) hemolytic and bacteriolytic plaque assays. it ...	1976	782974
biological effects of escherichia coli lipopolysaccharide (lps) in vivo. ii. selection in the mouse thymus of pha- and con a-responsive cells.	the effect of lps injection on mouse thymocytes was examined by in vitro stimulation with pha and con a. thymocytes from lps treated mice were found more responsive than normal thymocytes to pha and con a. the increase of responsiveness was different for pha and con a, and dependent on the dose of lps injected. this dose dependency suggests that lps in vivo modifies the thymocyte population by progressively eliminating t1 cells and selecting t2 cells. these effects seem to be mediated by cortico ...	1976	783042
an in vitro immuno-enzymatic assay of tumor antigens in the mouse with beta-galactosidase.	a method for detection of the primary binding of soluble tumor-associated antigens by antibodies has been developed by using an enzyme immunoassay (eia). a heteroantiserum was produced by injecting tumor cells from a chemically induced murine sarcoma into rabbits, and antibodies reacting with most normal tissue components were removed by exhaustive in vivo absorption. a soluble preparation of tumor cells, obtained by 3 m kcl extraction, was conjugated to beta-galactosidase from escherichia coli. ...	1976	784874
etiologic diagnosis of diarrheal disease of calves: frequency and methods for detecting enterotoxin and k99 antigen production by escherichia cola.	escherichia coli isolated from calves in minnesota and montana were tested for enterotoxigenicity via bio-assay of cell-free broth culture fluid and for k99 antigen via a serum agglutination test. infant mice were used to assay for heat-stable enterotoxin (st), and adrenal cells in culture were used to assay for heat-labile enterotoxin (lt). forty-six of the 345 e coli isolates produced st enterotoxin, but none produced lt enterotoxin. thirty-five of the 46 enterotoxigenic isolates had k99 antig ...	1976	786085
separation of transfer ribonucleic acids on polystyrene anion exchangers.	the transfer rna separation by chromatography on strong-base polystyrene exchange materials is examined and compared with the widely used reversed-phase chromatography. results indicate important differences in some transfer rna (trna) elution patterns by the anion-exchange chromatography, as compared with the reversed-phase chromatography. transfer rnas containing hydrophobic groups are adsorbed more strongly. the anion exchanger has twice the number of theoretical plates. single peaks of trna2 ...	1976	791362
dual pathway of b lymphocyte differentiation in vitro.	direct visualization of the events resulting from lps stimulation of mouse spleen cells in vitro was achieved by characterizing the cells during four days of culture for morphology, ig and theta surface markers and autoradiography after [3h] thymidine uptake. the changes observed were related to biochemical parameters such as incorporation of [3h] thymidine into dna, ig biosynthesis and secretion. two pathways of b lymphocyte differentiation were observed: a) the generation of a large number of ...	1976	791655
genetic control of b cell activation by bacterial lipopolysaccharide is mediated by multiple distinct genes or alleles.	eleven closely related c3h mouse strains were examined for differences in their dna synthetic response to a highly discriminatory endotoxin, lps escherichia coli k-235. strains of the c3h line were found to be either high, intermediate, or low responders to endotoxin. three stains, c3h/st, c3h/bi, and c3h/cr were high responders. six strains, c3h/he, c3hfb/he, c3h/hen, c3heb/fej, c3h/disn, and c3h/avy were intermediate responders, suggesting that a mutation producing decreased lps responsiveness ...	1976	792337
characterization of the rna transcribed in vitro from native mammalian dna by escherichia coli rna polymerase.	high-molecular-weight native mouse dna was transcribed with escherichia coli rna polymerase under low salt conditions, and the nature of the dna sequences transcribed determined by molecular hybridization. the results indicated that e. coli rna polymerase does not transcribe the sequences in native mouse dna randomly under these conditions. first, hybridization with a large excess of mouse dna showed that no more than 5% of the rna synthesized had been transcribed from repeated sequences in the ...	1976	793629
modification of resistance of mice to naegleria fowleri infections.	naegleria fowleri, which produces a fatal meningoencephalitis in humans, is also able to produce a progressive and fatal disease in mice. the course of the disease in dub/icr mice is dependent upon the infecting dose of organisms, whether administered intraperitoneally (i.p.) or intravenously (i.v.). all of the mice receiving 10(7) trophozoites/mouse i.v. or 4.85 x 10(7) trophozoites/mouse i.p. were killed within 10 days. escherichia coli o26:b6 lipopolysaccharide, administered at a dose of 1 mg ...	1976	1270145
mitogen-stimulated glutaraldehyde-fixed spleen cells: ability to stimulate in the mixed lymphocyte reaction and generate effector cells in cell-mediated lympholysis.	mouse spleen cells treated with glutaralde lose their stimulating ability in the mlr. if the spleen cells are first converted to a blastogenic state by lipopolysaccharide and subsequently fixed with glutaraldehyde, their stimulating capacity is maintained.	1976	135809
biological effects of escherichia coli lipopolysaccharide (lps) in vivo. i. selection in the mouse thymus of killer and helper cells.	in the present study we have investigated the biological effects on thymus lymphocytes resulting from escherichia coli lipopolysaccharide (lps) treatment in young adult mice. it has been established that lps induces the following effects: (a) a dose-dependent reduction of thymus weight contemporaneous with a rise in the anti-lps antibody response; (b) an increase of killer activity of thymus cells; (c) an enhancement of thymocytes helper activity; (d) a reduction of theta-positive cells in the t ...	1976	8378
formation of mutagenic n-nitroso compounds from the pesticides prometryne, dodine and carbaryl in the presence of nitrite at ph 1.	environmental chemicals including pesticides carrying secondary and tertiary amino groups are suggested to be a health hazard to man since potentially carcinogenic nitroso compounds may be formed in the presence of nitrite at low ph values resembling conditions in the human stomach. nitrosation of the isopropylamino-triazine prometryne, the n-dodecyl guanidine dodine and the n-methylcarbamate carbaryl was investigated in the presence of hcl and acetic acid at ph 1 and excess sodium nitrite for 4 ...	1976	12468
enterotoxigenic escherichia coli and reovirus-like agent in rural bangladesh.	48 patients admitted to a rural bangladesh hospital with dehydration secondary to diarrhea were examined for infection caused by (r.l.a.) reovirus-like agent or (e.t.e.c.) enterotoxigenic escherichia coli. the diagnosis of r.l.a. infection was established by electron microscopy of stool filtrates and by a 4-fold or greater rise in serum complement-fixing antibodies to the nebraska calf diarrhea virus. evidence of infection by heat-labile-toxin producing e.t.e.c. was sought by stool culture and ...	1976	73638
interferon-producing capacity of germfree mice.	the general capacity of germfree mouse spleen cells to produce interferon in vitro in response to various stimuli was investigated. the interferon response of germfree mouse spleen cells in vitro, when compared with that of the conventionals, appears to be lower to some inducers. interferon production in vitro stimulated by hemagglutinating virus of japan (hvj) or bhk-hvj cells (bhk cells persistently infected with hvj) was apparently suppressed in germfree mouse spleen cells as compared with th ...	1976	177363