Publications

[the effect of human papilloma virus extracts on rabbit endothelial explants].		1963	14064685
a study of human papilloma virus dna.		1965	4286353
primary tissue culture of human wart-derived epidermal cells (keratinocytes).	the formation of human skin warts may depend on transformation by human papilloma virus or result from a productive virus infection. to explore this problem, methods were developed for the primary tissue culture of epidermal cells (keratinocytes) derived from wart tissues and normal skin. wart-derived keratinocytes continued to proliferate for periods exceeding 3 months, but the patterns of cell growth were identical to normal keratinocytes. electron microscopic and immunohistologic examination ...	1975	164558
human papillomavirus dna: physical map.	human papillomavirus (hpv) dna form i (supercoiled) was prepared from plantar warts. hpv dna was cleaved with restriction enzymes obtained from the following sources: escherichia coli (ecori), hemophilus influenzae strain rd (both unfractionated hind and aeparated hindii and hindiii enzymes) and hemophilus parainfluenzae (hpai). the cleavage products were analyzed by polyacrylamide gradient slab gel electrophoresis and electron microscopy. hpv dna was cleaved into two fragments by ecori (87% and ...	1975	174077
[human papilloma virus (author's transl)].		1976	189105
chromatin-like structures obtained after alkaline disruption of bovine and human papillomaviruses.	four low-molecular-weight polypeptides migrating like h2a, h2b, h3, and h4 calf liver histones were detected by sodium dodecyl sulfate-acrylamide gel electrophoresis of highly purified preparations of bovine papillomavirus (bpv) and human papillomavirus (hpv). complexes of these polypeptides and viral dna were isolated by agarose-gel filtration of the alkaline disruption products of both viruses. when observed under the electron microscope, these complexes appeared as circular structures compose ...	1977	191643
chromatin-like structures obtained after alkaline disruption of bovine and human papillomaviruses.	four low-molecular-weight polypeptides migrating like h2a, h2b, h3, and h4 calf liver histones were detected by sodium dodecyl sulfate-acrylamide gel electrophoresis of highly purified preparations of bovine papillomavirus (bpv) and human papillomavirus (hpv). complexes of these polypeptides and viral dna were isolated by agarose-gel filtration of the alkaline disruption products of both viruses. when observed under the electron microscope, these complexes appeared as circular structures compose ...	1977	191643
human papillomavirus dna: physical mapping of the cleavage sites of bacillus amyloliquefaciens (bami) and haemophilus parainfluenzae (hpaii) endonucleases and evidence for partial heterogeneity.	the dna of human papillomavirus (hpv) obtained from a pool of plantar warts is cleaved by bacillus amyloliquefaciens (bami) and haemophilus parainfluenzae (hpaii) restriction endonucleases at one and four specific sites, respectively. these sites were localized on the previously established cleavage map of hpv dna, using the hind, hindiii, hpai, and ecori endonuclease restriction sites as reference. the four hpaii sites were mapped, clockwise, at 1.4, 41.1, 44.3, and 52.8% of the genome length f ...	1977	191644
identification and characterization of a papillomavirus from birds (fringillidae).	from skin papillomas of the chaffinch (fringilla coelebs), a virus has been purified and studied by physicochemical techniques and electron microscopy. the virions measure 52 nm in diameter and are composed of 72 morphological units arranged in a skew t = 7d surface lattice. a sedimentation coefficient of about 300s and a buoyant density of 1.34 g/ml in cscl were determined for the particle. its protein composition resembles that of human papillomavirus, and the circular double-stranded genome m ...	1977	197046
inverted repetitive sequences in human papilloma virus 1 (hpv-1) dna.		1977	201085
identification of an immunologically distinct papillomavirus from lesions of epidermodysplasia verruciformis.	virions isolated from warts of 2 siblings with epidermodysplasia verruciformis (ev), a rare disease characterized by the lifelong growth of warty skin tumors containing papova-like virions, were compared to isolates of human papillomavirus (hpv) from 3 pools of plantar and common hand warts. the length of relaxed, circular (form ii) molecules of ev virion dna approximated the length of hpv dna but was 3.3% longer. antisera prepared in rabbits against the 3 hpv pools coated and aggregated hpv in ...	1977	71354
characterization of human papilloma virus (h.p.v.) present in genital warts.		1978	205177
so-called multicentric pigmented bowen's disease. report of a case and a possible etiologic role of human papilloma virus.	multicentric pigmented bowen's disease (mpbd) is a bowenoid atypia in the genitocrural region with peculiar clinical appearances. a 36-year-old japanese female patient showed a variety of lesions. clinically the lesions on the external genitalia consisted of brown-black papillomatous eruptions, black discrete or confluent papules, and whitish macerated papules. histologically only black papules showed bowenoid atypia, but whitish papules also showed transient bowenoid atypia. electron microscopi ...	1978	211056
seroepidemiologic studies of bovine papillomavirus infections.	bovine and human sera were analyzed for the presence of antibodies against bovine papillomavirus types 1 and 2 (bpv 1 and 2) and human papillomavirus type 1 (hpv 1) in a solid-phase radioimmunoassay. human sera did not react with bpv antigens, and bovine sera showed no evidence of antibodies against hpv 1. in contrast, 19% of all bovine sera tested reacted with bpv 1 and 2 antigens, and 35% of human sera revealed antibodies against hpv 1. no serologic evidence was obtained for heterologous infec ...	1979	220453
seroepidemiologic studies of bovine papillomavirus infections.	bovine and human sera were analyzed for the presence of antibodies against bovine papillomavirus types 1 and 2 (bpv 1 and 2) and human papillomavirus type 1 (hpv 1) in a solid-phase radioimmunoassay. human sera did not react with bpv antigens, and bovine sera showed no evidence of antibodies against hpv 1. in contrast, 19% of all bovine sera tested reacted with bpv 1 and 2 antigens, and 35% of human sera revealed antibodies against hpv 1. no serologic evidence was obtained for heterologous infec ...	1979	220453
localization of viral dna-replication in sections of human warts by nucleic acid hybridization with complementary rna of human papilloma virus type 1.	using complementary rna of human papilloma virus type 1 (hpv1) and in situ hybridization techniques the localization of viral dna replication was studied in sections of 38 human virus acanthomata from 31 different patients. in five cases significant labeling was detected by autoradiography. labeling started always in the first or the second suprabasal cell layer and was strongly limited to the nuclei. a remarkable early beginning of the vacuolated process seemed to be correlated with the visible ...	1979	220921
connection between capsomeres in human papilloma virus.		1979	223316
characterization of human papillomavirus 3 in warts of a renal allograft patient.	multiple flat wart-like lesions of a renal allograft recipient were shown to contain hpv 3 or a serologically crossreacting virus by indirect immunofluorescence with monospecific animal antisera against hpv [1--5]. the patient's serum revealed virus specific antibodies (immunofluorescence titer 1/80). papillomaviruses were isolated and after in vitro iodination 3 major proteins (mw 70.000, 56.000 and 43.000) were detected by sds polyacryalmide gel electrophoresis. dna was extracted from the wart ...	1979	227966
immunology of human warts.	rapid advances have occurred in the characterization of human papilloma virus (hpv) types applying the new advanced techniques of restriction endonuclease analysis and molecular hybridization to human wart virus. human papilloma virus can no longer be viewed as a single, homogeneous virus producing all varieties of clinical warts. at least three antigenically heterogeneous hpv types have been associated with common and plantar warts. two additional hpv types have been found in patients with epid ...	1979	229134
immunoperoxidase localization of human papillomavirus in laryngeal papillomas.	five antigenically distinct serotypes of human papillomavirus (hpv) cause various cutaneous verrucae with distinct clinical features. laryngeal and other mucosal papillomas, also suspected to have a viral etiology, were examined for the presence of a genus-specific (common) antigen of hpv using a peroxidase-antiperoxidase (pap) technique. the primary antiserum used was reactive against laryngeal papillomas from 26 of 35 randomly selected patients (74%); overall, 49 of the 102 squamous papilloma ...	1980	6263818
human papilloma virus infection of the uterine cervix.		1980	6243835
oral retinoid treatment of human papillomavirus type 5-induced epidermodysplasia verruciformis.		1980	6245361
molecular cloning, refined physical map and heterogeneity of methylation sites of papilloma virus type 1a dna.	the entire genome of human papilloma virus type 1a was cloned in escherichia coli using the plasmid pbr322 as vector. the integrity and the homogeneity of the viral dna thus obtained was confirmed by restriction endonucleases analysis. viral dna isolated from a single wart was partially methylated at only one out of the four hpaii sites, d(c-c-g-g). recognition sites for bg/i, bg/ii, psti and pvuii restriction endonucleases were located on the cloned genome.	1980	6250842
a potentially oncogenic human papillomavirus (hpv-5) found in two renal allograft recipients.	we have observed 2 immunosuppressed renal allograft recipients with skin lesions induced by human papillomavirus type 5 (hpv-5). one recipient had multiple pityriasis versicolor-like (pv-like) skin lesions on his arms and trunk, and multiple bowenoid in-situ skin cancers. the other had 2 warty lesions on the back of her fingers. structural antigens of human papillomavirus type 5 (hpv-5) were identified in benign lesions from both patients by immunofluorescence. the histologic and ultrastructural ...	1980	6253573
cloning of human papilloma virus genomic dnas and analysis of homologous polynucleotide sequences.	the complete dna genomes of four distinct human papilloma viruses (human papilloma virus subtype 1a [hpv-1a], hpv-1b, hpv-2a, and hpv-4) were molecularly cloned in escherichia coli, using the certified plasmid vector pbr322. the restriction endonuclease patterns of the cloned hpv-1a and hpv-1b dnas were similar to those already published for uncloned dnas. physical maps were constructed for hpv-2a dna and hpv-4 dna, since these viral dnas had not been previously mapped. by using the cloned dnas, ...	1980	6253665
relationship between condylomata and laryngeal papillomata. clinical and molecular virological evidence.	in a survey of 49 papilloma patients accurate maternal condyloma history was obtained in 31 instances and of these, 21 were positive for the presence of condyloma during pregnancy or parturition. molecular virological studies indicate that positive hybridization could be demonstrated to human papilloma virus 2 in both laryngeal papilloma and condyloma by the southern blot technique. immunoperoxidase staining illustrated the presence of virus-related particles only near the surface of the mucous ...	1980	6254426
anogenital warts contain several distinct species of human papillomavirus.	anogenital warts from 26 patients were examined for the presence of human papillomavirus (hpv). although no whole, intact virus could be identified, varying amounts of nonintegrated hpv dna were detected in 18 tissue specimens (70%) by employing both an agarose gel-ethidium bromide staining method and the southern blot hybridization procedure. when hybridization analysis was performed under stringent conditions, six anogenital warts were observed to contain hpv genomic sequences related to eithe ...	1980	6255208
presence of human papilloma viral antigens in juvenile multiple laryngeal papilloma.	juvenile laryngeal papillomas, solitary laryngeal papillomas of the adult, and cylindric cell papillomas of the nose and sinuses were examined for the presence of papillomavirus antigens by means of immunocytochemistry. by using an antiserum capable of recognizing a common group antigen that reacts with papillomavirus antigens of different species, it was found that half of the juvenile laryngeal papillomas studied contained cells staining for papillomavirus antigens. no positive cells were foun ...	1981	6258422
histological evidence for the presence of condylomatous epithelial lesions in association with laryngeal squamous cell carcinoma.	the laryngeal specimens obtained from 116 patients with established invasive squamous cell carcinoma of the larynx were histologically assessed with special reference to the detection of morphological manifestations of human papilloma virus (hpv), as newly described in the uterine cervix, i.e. the three different types of condylomas (the papillomatous, the flat and the inverted ones). epithelial changes fulfilling the criteria of the papillomatous condyloma were found in 15 cases, those of the i ...	1981	6265845
human papilloma virus type i purified from human genital warts.	human wart virus (hpv) was isolated from a pool of genital warts. the electrophoretic mobility of virion proteins was studied by sds polyacrylamide gel electrophoresis and showed the same pattern as that obtained with hpv-1. the analysis of dna after restriction enzyme digestion with the endonucleases hind iii and hae iii, and nucleic acid hybridization did not show any difference with hpv-1. the viral particles were agglutinated by anti-hpv-1 serum, as shown by electron microscopic particle agg ...	1981	6266889
genital warts.	human papillomaviruses have not been propagated in tissue culture, so they cannot be studied by the procedures of classical virology. recently, dna hydridization techniques and restriction enzyme analysis have revealed that multiple strains of human papillomaviruses exist. it is now accepted that genital warts are a sexually transmitted disease; other routes of infection are possible, but unusual. an association between maternal warts during pregnancy and the development of vulval warts and lary ...	1981	6277022
vulvar intraepithelial neoplasia: correlation of nuclear dna content and the presence of a human papilloma virus (hpv) structural antigen.	immunoperoxidase localization of a human papilloma virus structural antigen (hpv) was attempted in 68 intraepithelial lesions of the vulva, 39 of which were analyzed for nuclear dna content by microspectrophotometry. overall, 5.9% (4/68) stained positive for hpv. ninety percent (35/39) of the cases tested were aneuploid, and, of these, 2.8% (1/35) stained positive for hpv. in contrast, 50% (2/4) of the polyploid lesions were positive. hence dna microspectrophotometry and immunoperoxidase localiz ...	1982	6277449
analysis of human genital warts (condylomata acuminata) and other genital tumors for human papillomavirus type 6 dna.	32p-labelled cloned hpv 6 dna was used as probe to analyze human genital tumors for dna sequences homologous to hpv 6 dna. ninety three percent of all condylomata acuminata (41 out of 44) were found to harbor hpv 6 dna. of the remaining three, one contained hpv 1 dna. no papillomavirus dna was identified in the two other tumors. all three invasively growing giant condylomata acuminata (buschke-löwenstein tumors) investigated also contained hpv 6 dna. two out of six atypical condylomata of the ce ...	1982	6277807
human papillomavirus dna in cutaneous primary and metastasized squamous cell carcinomas from patients with epidermodysplasia verruciformis.	dna extracted from squamous cell carcinomas from patients with the chronic wart disease syndrome, epidermodysplasia verruciformis, was analyzed for the presence of human papillomavirus (hpv)-specific dna sequences by southern blot hybridization analysis. employing an hpv probe obtained by molecular cloning of viral dna purified from benign warts from these patients, we have unequivocally identified hpv-specific nucleotide sequences in squamous cell carcinomas from these patients. restriction end ...	1982	6280194
new human papilloma virus isolated from epidermodysplasia verruciformis lesions.	human papilloma virus (hpv) was isolated from red plaques of a patient (n. f.) with epidermodysplasia verruciformis. electron microscopic examination showed characteristic particles of papilloma virus as icosahedrons about 45 nm in diameter. dna was extracted from these particles, and closed-circular dna (form i) was purified by centrifugation in cscl containing ethidium bromide. the molecular weight of the dna was about 5.0 x 10(6). a physical map of the hpv dna was constructed using several re ...	1982	6280858
infections by human papillomavirus (warts).		1982	6282150
genital warts and cervical cancer. i. evidence of an association between subclinical papillomavirus infection and cervical malignancy.	a blind comparative survey was undertaken to study the prevalence of subclinical papillomavirus infection (spi) in a representative sample of women treated surgically for invasive or preinvasive cervical neoplasia. according to a semiobjective rating system, 73 of 80 women (91%) with cervical neoplasia and ten of 80 matched controls (12.5%) showed histologic evidence of human papillomavirus (hpv) infection. sixty of the controls (75%), but none of the study group, had normal cervicovaginal epith ...	1982	6282442
a comparative study on ultrastructure and immunological properties of human papilloma virus before and after fixation.		1982	6286744
demonstration of human papilloma virus antigen in the condylomatous lesions of the uterine cervix by immunoperoxidase technique.	routine histological sections of characteristic condylomas (showing dysplasia of different degrees) collected from the uterine cervix of 10 women were subjected to staining with indirect immunoperoxidase technique using antihuman papillomavirus (anti-hpv) serum to demonstrate the hpv antigens in these lesions. the anti-hpv immune serum was raised in guinea pigs immunized with highly purified viruses from a pool of skin wart lesions collected from various anatomical sites. 8 out of the 10 condylo ...	1982	6288519
human papilloma virus infection (condyloma) of the uterine cervix in japanese women.		1982	6290429
viral etiology of juvenile- and adult-onset squamous papilloma of the larynx.	juvenile- and adult-onset laryngeal papillomas were examined for the presence of a human papillomavirus (hpv) genome and capsid antigens. dna was isolated from a portion of tissue removed for therapeutic purposes, and the presence of a papillomavirus genome was detected by southern transfer analysis. the viral dna found in the 12 juvenile-onset and the 8 adult-onset laryngeal papillomas examined was identified as hpv-6 on the basis of size, restriction endonuclease digestion patterns, and homolo ...	1982	6291043
[clinical and histological characteristics of the changes induced by human papilloma virus (hpv 7)].		1982	6312492
carcinoma cuniculatum (verrucous carcinoma of the skin): a clinicopathologic study of 46 cases with ultrastructural observations.	forty-six examples of carcinoma cuniculatum, a rare variant of squamous cell carcinoma of the skin, were studied by means of light and electron microscopy and immunoperoxidase techniques. the tumors usually presented as ulcerated, fungating, and polypoid masses with openings of sinus tracts onto the skin surface that exuded foul-smelling greasy material when pressure was applied. on microscopic examination the tumors were seen to be composed mainly of mature squamous keratinocytes with foci of c ...	1982	7042070
histological changes identical to those of condylomatous lesions found in esophageal squamous cell carcinomas.	esophageal specimens derived from sixty patients with an established invasive squamous cell carcinoma of the esophagus were histologically surveyed with special reference to the morphological manifestations of human papilloma virus (hpv), known to cause flat, inverted and papillomatous lesions (condylomas) in the uterine cervix. epithelial changes fulfilling the criteria of the papillomatous condyloma were present in one case, those of the inverted lesions in three cases, and those of the flat c ...	1982	7138240
identification of human papillomavirus dna in cervical and vaginal intraepithelial neoplasia with molecularly cloned virus-specific dna probes.	the presence of human papillomavirus (hpv) dna was identified in the tissues of cervical and vaginal intraepithelial neoplasia by southern blot dna hybridization under conditions of low stringency. the specific types of hpv present in the tissues were identified by using molecularly cloned types 1 through 6 (hpv-1 through hpv-6) hpv dna probes under high-stringency conditions. all tissues of cervical and vaginal intraepithelial neoplasia analyzed contained hpv genomes. fifteen of 19 samples (79% ...	1983	6313534
the story of a cell. the george n. papanicolaou award lecture.	the history of one cell type observed on gynecologic cytologic samples and later identified as pathognomonic of human papillomavirus (hpv) infection is presented. mention is also made of the role of hpv in the development of premalignant and malignant squamous lesions of the uterine cervix.	1983	6316699
human papillomavirus: detection of viral dna sequences and evidence for molecular heterogeneity in metaplasias and dysplasias of the uterine cervix.	colposcopic-directed biopsies of the uterine cervix from 22 patients were analyzed for the presence of human papillomavirus (hpv) dna and structural antigens. 11 of the biopsies were classified microscopically as mild dysplasia, 3 as moderate dysplasia, 1 as severe dysplasia, and 7 as squamous metaplasia. nonstringent hybridization with a bovine papillomavirus type 1 dna probe and immunocytochemical analysis with an antiserum against papillomavirus genus-specific structural antigens were perform ...	1983	6317605
human papillomavirus (hpv) venereal infections and gynecologic cancer.		1983	6326025
[focal epithelial hyperplasia in lepromatous leprosy].	focal epithelial hyperplasia heck (feh) is most likely caused by human papilloma virus. it mainly occurs in children and young people showing no associated diseases. for the first time, we describe a case of feh in a patient with lepromatous leprosy who due to persistent erythema nodosum leprosum has been treated with a lang-term glucocorticoid therapy. the question of the competence of lepromatous patients in resisting certain viral infections arises.	1983	6649746
human papilloma virus infection (condyloma) of the cervix and cervical intraepithelial neoplasia: a histopathologic and statistical analysis.	two hundred fifty consecutive cases previously diagnosed as advanced intraepithelial neoplasia (cin 2 and cin iii) were reviewed and examined for the presence of coexisting condyloma. when the mean ages and age distributions were analyzed, there was a consistent shift of the age distribution to a younger age among women with cin and condyloma as opposed to cin alone. furthermore, when the relationship between age and case accumulation was normalized from a curvilinear to a linear relationship, t ...	1983	6822369
effect of oral aromatic retinoid (ro 10-9359) on human papilloma virus-2-induced common warts.	persisting human papilloma virus (hpv) 2 induced common warts of a chronic lymphatic leukemia patient were orally treated with aromatic retinoid ro 10-9359 (tigason). clinically, the lesions improved rapidly. virus-specific cytopathogenic effects (cpe), virus particles and viral dna were no longer detectable. therapy was discontinued because of the development of a liposarcoma, which led to a complete relapse of the cutaneous lesions. hpv-2 specific parameters, cpe and viral dna, were restored. ...	1983	6840376
[false forms of bowen disease of the genital mucosa].	this review deals with the lesions of the genitalia histologically mimicking bowen's disease, yet behaving like a benign process. the report is based on data from the literature and on 14 personal cases, four of which are described here as examples. some of the cases, mostly young patients, showed spontaneous regression; in others condylomata acuminata developed on the site of the lesions; in rare instances a "transformation" in lichen planus occurred. for these not really precancerous condition ...	1983	6874339
cervical papillomavirus infection in diethylstilbestrol-exposed progeny.	histologic sections from cervical and vaginal biopsies showing dysplasia in 37 women exposed to diethylstilbestrol (des) in utero were stained for human papillomavirus structural antigens using an immunoperoxidase technique. forty-three percent of the lesions had detectable papillomavirus antigens. these findings indicate that a significant proportion of cervical dysplasia observed in des-exposed progeny is associated with papillomavirus infection.	1983	6296741
human papilloma virus 5-dna in a carcinoma of an epidermodysplasia verruciformis patient infected with various human papillomavirus types.	an epidermodysplasia verruciformis patient suffering from generalized warts and a carcinoma at the forehead was found to be infected by at least six types and subtypes of human papillomaviruses. the central part of the carcinoma, however, harbored only human papillomavirus 5 dna. the dna persisted extrachromosomally in high genome copy number. in contrast to wart dna preparations, a significant part of the viral sequences in the carcinoma was present as oligomers, at least part of them being con ...	1983	6297727
abnormalities of the uterine cervix in women with vulval warts. a preliminary communication.	fifty women attending a clinic for sexually transmitted diseases with vulval condylomata acuminata were examined by cervical cytology and colposcopy for cervical infection by human papillomavirus (hpv) or epithelial abnormality indicating cervical intraepithelial neoplasia (cin) or both. collated results showed a high prevalence of both conditions in these 50 women; 25 (50%) had evidence of cervical infection by hpv and 18 (36%) epithelial abnormalities consistent with cin 1 or 2.	1983	6299450
human papillomavirus types 6 and 11 dna sequences in genital and laryngeal papillomas and in some cervical cancers.	human genital tumors as well as recurrent laryngeal papillomas were analyzed for the presence of human papillomavirus (hpv) 6 and hpv 11 sequences. hpv 11 dna was found in 7 of 14 laryngeal papillomas; in the 7 other tumors no hpv dna was demonstrated. hpv 11 dna was also found in all five atypical condylomata of the cervix included in this study. condylomata acuminata mainly contained hpv 6 dna. from 63 biopsy specimens, 41 clearly harbored hpv 6 dna and 13 harbored hpv 11 dna. in three tumors ...	1983	6300854
human papilloma virus linked to cervical (and other) cancers.		1983	6304359
a papillomavirus dna from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions.	dna from one biopsy sample of invasive cancer of the cervix contained sequences hybridizing with human papillomavirus (hpv) type 11 dna only under nonstringent conditions. this dna was molecularly cloned in lambda phage. under stringent conditions of hybridization it cross-hybridized to a minor extent (less than 0.1%) with hpv types 10, 14, and 15 and showed no homology with dna of other human hpv types. we therefore propose to designate it tentatively as hpv 16. hpv 16 dna was used as a probe t ...	1983	6304740
human papillomavirus (hpv) involvement in squamous cell lesions of the oral cavity.		1983	6306646
the prevalence of human papillomavirus antigen in patients with cervical intraepithelial neoplasia.		1983	6307333
human papilloma virus in multifocal, invasive female genital tract malignancy.	two young patients with multifocal, invasive, verrucoid carcinomas of the lower genital tract are described. viral particles consistent with human papilloma virus were demonstrated by transmission electron microscopy in areas of koilocytotic malignant and nonmalignant epithelium, and both patients had evidence of impaired immune function. the role of human papilloma virus in genital neoplasia is discussed.	1983	6308532
oncological consequences of impaired immune surveillance against ubiquitous viruses.	the immune system is constantly challenged by ubiquitous viruses. multiple immune defenses have evolved to meet these challenges, and thus immunocompetent individuals successfully respond to infection without sequela. x-linked lymphoproliferative syndrome patients, renal allograft recipients, and acquired immunodeficiency syndrome patients share impaired immune surveillance as a common feature. such individuals are variously susceptible to numerous untoward complications following infection with ...	1983	6309892
natural history of cervical epithelial abnormalities in patients with vulval warts. a colposcopic study.	the natural history of intraepithelial abnormalities of the cervix associated with human papillomavirus infection was investigated in a prospective study of 50 women with vulval warts, of whom 28 had colposcopic evidence of a cervical epithelial abnormality and 22 a normal cervix. of the 28 with a cervical abnormality, 26 were re-examined by colposcopy after three months; the epithelial abnormality had persisted in 23 women. nineteen women who had initially shown abnormality by colposcopy were r ...	1983	6311324
characterization of human papillomavirus type 13 from focal epithelial hyperplasia heck lesions.	focal epithelial hyperplasia heck lesions of a turkish patient were shown to contain papillomavirus-specific dna, which was molecularly cloned into bacteriophage lambda. it proved to be related to human papillomavirus (hpv) type 6 dna and hpv type 11 dna. reassociation kinetics revealed a cross-hybridization of 4 and 3%, respectively. there was no cross-reactivity with hpv type 1, 2, 3, 4, 5, 8, or 10. this papillomavirus type will be referred to as hpv type 13. the dna was characterized by clea ...	1983	6312071
immunology of human papillomavirus: warts.	great progress has been made over the last five years in our understanding of papillomavirus (pv) biology. new technology has enabled investigators to understand the relationship between the pv and its host. the pv cannot be cultured in vitro, and this has led to limitations for those wishing to study the biology of this virus. however, utilizing recombinant dna technology, investigators now have abundant quantities of human papillomavirus (hpv) dna for study. such hpv genomes may be labeled wit ...	1983	6095237
morphological and immunohistochemical evidence of human papilloma virus (hpv) involvement in the dysplastic lesions of the uterine cervix.	a series of 110 biopsies of the uterine cervix containing dysplastic and/or in situ changes were assessed morphologiccally with reference to presence or absence of the coexistent condylomatous lesions, and using the immunoperoxidase-pap technique to disclose the hpv (human papilloma virus) antigens in the cells. morphologically, 79 biopsies contained condylomatous (co) lesions (63 flat, 12 inverted and 4 papillomatous ones), and 31 were non-condylomatous (nco) dysplasias/in situ carcinomas. the ...	1983	6141079
langerhans cells in human papillomavirus (hpv) lesions of the uterine cervix identified by the monoclonal antibody okt-6.	the local inflammatory cell infiltrates in 263 cervical punch biopsies of the women followed-up since 1981 (16 +/- 14 months, mean +/- s.d.) for an established human papillomavirus (hpv) lesion with or without concomitant cervical intraepithelial neoplasia (cin) were analysed for occurrence of langerhans cells, defined by the monoclonal antibody okt-6 using the avidin-biotin peroxidase complex (abc) technique. okt-6+ cells remained at the constant low level (1.5-1.9% of the inflammatory cells) i ...	1984	6151923
human papillomavirus dna in genital tumours.	human papillomaviruses (hpv) types 16 and 18 have been identified in two different human cervical carcinomas. the viral dnas were molecularly cloned and used as probes to screen a large number of genital tumours by southern blot analysis. hpv 16 or hpv 18 sequences were found in a high percentage of cervical carcinomas but only in a small number of condylomata acuminata or flat condylomas. the majority of the latter lesions, however, contained hpv 6 or hpv 11 sequences, respectively, which, in c ...	1984	6100278
monoclonal antibodies to the major capsid protein of human papillomavirus type 1.	two stable monoclonal hybridoma cell lines secreting type-specific antibodies against the human papillomavirus type 1 (hpv-1) were isolated. the monoclonal antibodies detected hpv-1 antigens in frozen sections of hpv-1-induced warts, using immunofluorescence or immunoperoxidase techniques, and they reacted with hpv-1 particles in an immunodiffusion test. the two monoclonal antibodies recognized the major structural viral polypeptide, with a molecular weight of 54 000, and a minor polypeptide, wi ...	1984	6086818
the koilocyte and cervical intraepithelial neoplasia: time-trend analysis of a recent decade.	to determine whether the role of the human papillomavirus in the development of cervical intraepithelial neoplasia has changed recently, cervical biopsy specimens from the years 1972 and 1982 were reviewed to compare the frequency of diagnosis of human papillomavirus-associated koilocytosis in patients with suspected cervical intraepithelial neoplasia. no significant difference was found in the frequency of koilocytosis in biopsy specimens with cervical intraepithelial neoplasia between the year ...	1984	6091461
[cytologic diagnosis of cervico-vaginal infections with herpesvirus hominis type ii and human papilloma virus].		1984	6091487
molecular cloning and characterization of the genomes of nine newly recognized human papillomavirus types associated with epidermodysplasia verruciformis.	the genomes of 11 human papillomaviruses (hpvs) found in benign lesions of eight patients suffering from epidermodysplasia verruciformis were cloned in escherichia coli after insertion into plasmid pbr322. the study of the sensitivity of the cloned hpv dnas to 14 restriction endonucleases permitted the construction of physical maps. dna-dna hybridization experiments, performed under stringent conditions, showed that these viruses represent nine new types, hpvs 14 (with subtypes a and b), 15, 17 ...	1984	6092701
[focal epithelial hyperplasia (heck disease). contribution on its viral origin].	demonstration of human papilloma virus dna (hpv-dna) in the lesions of focal epithelial hyperplasia in an 11-year-old turkish girl provides further proof of its viral cause. in this case characterization of hpv-dna showed a reaction with that of type 13 and 18. this previously undescribed variant is probably the expression of a new hpv type.	1984	6094135
cloning and characterization of papillomavirus type 2c dna.	human papillomavirus (hpv) dna was isolated from a clinically diagnosed flat wart and proved to be related to hpv2. the isolate showed 55% cross-hybridization with hpv2a. a physical map of restriction enzyme cleavage sites differed completely from those of hpv2a and hpv2b. the new hpv2 subtype, which will be classified as hpv2c, was found to be very prevalent in common warts.	1984	6094390
presence of human papillomavirus in verrucous carcinoma (ackerman) of the vagina. immunocytochemical, ultrastructural, and dna hybridization studies.	human papillomavirus (hpv) genomes were identified in two cases of verrucous carcinoma of the vagina, using southern blot dna hybridization under low-stringency conditions. type (group) 6 hpv dna (hpv-6) was identified, using molecularly cloned hpv-1 through hpv-6 dna probes under high-stringency conditions in both cases. in addition, dna extract in one case hybridized with hpv-1, hpv-3, and hpv-4 dna probes. no hpv structural proteins were demonstrated in either case by immunocytochemical tests ...	1984	6329128
papillomavirus infection of the cervix. iii: relationship of the presence of viral structural proteins to the expression of involucrin.	forty-two cervical biopsies with cervical intraepithelial neoplasia were compared with respect to the expression of human papillomavirus (hpv) structural proteins and the expression of the cellular structural protein involucrin, a marker of suprabasal squamous differentiation. hpv structural protein and involucrin expression displayed an inverse correlation with the severity of dysplasia. both of these proteins were detected in 11 of 28 cases (39%) of mild and moderate dysplasia, but in only two ...	1984	6329974
papillomavirus genomes: sequences and consequences.	the complete nucleotide sequences of four papillomavirus genomes recently determined (human papillomavirus types 1a and 6b, bovine papillomavirus type 1, and the shope cottontail rabbit papillomavirus) shed light on the molecular genetics of these epitheliotropic viruses. the organization of the four genomes is very similar, and their comparative analysis suggests new hypotheses about the nature of the viral proteins, their involvement in virus structure, and their interaction with the host cell ...	1984	6330226
hsv, cmv, and hpv in human neoplasia.	we are studying the role of sexually transmitted viruses in the development of human tumors. the persistence of herpes simplex virus, cytomegalovirus, and human papillomavirus nucleic acid sequences has been examined using cloned viral dna sequences as probes. the relationship of the viruses to various stages in the progression of neoplasia is examined, with particular reference to the role of viral and/or cellular genes in the initiation, promotion, and maintenance of the neoplastic phenotype. ...	1984	6330227
the pathology of superficially invasive, thin vulvar squamous cell carcinoma.	thirty-six cases of vulvar squamous cell carcinoma 5 mm or less in thickness were studied, and potential predictors of lymph node metastases were evaluated. tumor thickness and depth of stromal invasion were measured. inguinal lymph node metastases were present in six (17%) cases, all of which had primary neoplasms more than 3 mm thick. the most superficial lesion to have lymph node metastasis was 3.2 mm thick and had 1.6 mm of stromal invasion. nonetheless, depth of stromal invasion of less tha ...	1984	6511160
association of human papillomavirus subtype and clinical course in respiratory papillomatosis.	the relationship of human papillomavirus type 6 (hpv-6) subtypes to the clinical manifestations of respiratory papillomatosis was investigated. dna was isolated from biopsy specimens of 21 patients and the viral genome analyzed by molecular hybridization. four subtypes, designated hpv-6c through hpv-6f, were distinguishable by restriction endonuclease cleavage patterns of the viral genome. patient records were reviewed to identify associations between viral subtype and sex, race, age at onset of ...	1984	6318005
human papillomavirus infection and cervical intraepithelial neoplasia: histopathology and dna content.	to evaluate the reliability of diagnostic criteria for separating intraepithelial squamous lesions into low- and high-risk categories, 25 lesions of the cervix were diagnosed as flat condyloma, atypical immature metaplasia, or cervical intra-epithelial neoplasia with koilocytosis based on well-defined histologic criteria. the presumption was that flat condyloma and atypical immature metaplasia would be diploid/polyploid as compared to low-grade cervical intraepithelial neoplasia, which should be ...	1984	6318171
human papillomavirus infection of the uterine cervix: histological appearances in 28 cases identified by immunohistochemical techniques.	twenty eight biopsy specimens of the cervix showed positive immunohistochemical staining when treated with an antiserum raised against an internal capsid antigen of human papillomavirus (hpv). histological examination of adjoining sections from the same blocks showed a much wider range of abnormalities than those already described in association with hpv infection. the picture was usually diagnostic. it rested chiefly on identifying the koilocyte--the cell with the perinuclear halo that carries ...	1984	6319459
bowenoid papulosis. demonstration of human papillomavirus (hpv) with anti-hpv immune serum.	two cases of bowenoid papulosis (bp) occurred. the presence of human papillomavirus was demonstrated in one patient. in the other patient, condyloma acuminatum developed after initial spontaneous improvement in his lesions. the discovery of a possible oncogenic virus in bp and the epidemiologic behavior of carcinoma in situ and multicentric carcinomas suggest the need for a very careful follow-up evaluation of such lesions. bowenoid papulosis may be a chronic dysplasia with a variable long-term ...	1984	6322703
screening for wart virus infection in normal and abnormal cervices by dna hybridisation of cervical scrapes.	cervical scrapings from 78 women attending a sexually transmitted diseases (std) clinic, family planning clinic, and colposcopy clinic were assayed for human papillomavirus type 6 (hpv-6) by dna hybridisation. results of hybridisation were compared with clinical, colposcopic, and cytological findings. scrapings from 2 of the 4 women with genital warts gave positive results with the hpv probe. no hpv-6 dna was detected in scrapings from 18 women attending an fpc whereas scrapings from 2 of 19 std ...	1985	2857023
increased risk of cervical neoplasia in consorts of men with penile condylomata acuminata.	19 (76%) of 25 women who had been the sole sexual consorts, for at least 1 year, of men with pre-existing penile condylomata acuminata had similar lesions of the lower genital tract. 9 (36%) of the women had an abnormal cervix as judged by cytology, colposcopy, and histology, and 7 (77%) of these 9 had associated human papillomavirus (hpv) infection detected by dna/dna hybridisation. none of 20 women in an age-matched control group had cytological or colposcopic evidence of cervical epithelial a ...	1985	2859410
colposcopy in women with papillomavirus lesions of the uterine cervix.	colposcopic examinations for human papillomavirus lesions were performed in 271 women, some of whom had developed concomitant cervical intraepithelial neoplasia since 1981. the colposcopic appearance was classified into one of the following categories: normal, punctate, mosaic, warty, leukoplakial, or combination, and was related to findings in papanicolaou smears and punch biopsy specimens. there was a good correlation between the colposcopic appearance and the findings in the papanicolaou smea ...	1985	2983274
human papillomavirus type 16 related dna in an anaplastic carcinoma of the lung.	twenty-four biopsy specimens from various histologic types of human carcinomas in the lung were analyzed for the presence of human papillomavirus (hpv) dna. dna from the individual specimens was tested for the presence of homologous sequences to hpv genotypes 1, 2, 4, 8, 9, 10, 11, 13, 16 and 18. one anaplastic carcinoma in the lung contained multiple copies of dna hybridizing under stringent conditions to hpv 16 dna. the latter dna has been found to be frequently associated with human genital c ...	1985	2983861
[immunoperoxidase demonstration of papillomavirus antigen in dysplasia of the uterine cervix].	recently, it has been widely recognized that human papillomavirus (hpv) infection plays an important role in the etiology of carcinoma of the cervix. paraffin sections of cervical malignancies and premalignancies were screened for the presence of papillomavirus antigen by means of peroxidase-antiperoxidase (pap) method. no case of invasive carcinoma, carcinoma in situ or normal cervix revealed positive hpv staining. in 81 cases (18%) of 461 cervical dysplasias, positive reaction for hpv was dete ...	1985	2984298
[electron microscopic detection of viruses in cervix papilloma].	the authors describe the electron microscope findings in a case of papilloma of the uterine cervix. in the nuclei of single koilocytes spherical inclusions measuring 47-56 nm were found. the ultrastructural pattern of the particles is entirely identical with one of the human papilloma virus particles.	1985	2984862
papillomavirus infection of the lower genital tract: detection of viral dna in gynecological swabs.	a total of 311 smears from the lower genital tract were examined by the filter in situ hybridization method to identify human papillomavirus (hpv) dna. of these 311 smears, 229 came from clinically and cytologically negative patients and served as a control group. in this group hpv-dna was detected in 5 cases (2.2%). of 82 cytologically positive cases (25 confirmed by histology) 56 (68%) contained hpv-dna. a high prevalence of hpv 6/11 and absence of hpv 16/18 was found in cases with cytological ...	1985	2985509
analysis of oral papillomas, leukoplakias, and invasive carcinomas for human papillomavirus type related dna.	five papillomas, five leukoplakias, and six carcinomas were investigated for the presence of papillomavirus group-specific antigens and viral dna. viral proteins were identified with genus-specific papillomavirus antibodies. cloned human papillomavirus (hpv) 11 and 16 dna were used as probes in southern blot hybridization at conditions of different stringency in order to determine viral dna. four of five papillomas, four of five leukoplakias, and three of six carcinomas reacted with hpv dna prob ...	1985	2987361
introduction of cloned human papillomavirus 1a dna into rat fibroblasts: integration, de novo methylation and absence of cellular morphological transformation.	the morphologically normal rat fibroblast cell line rat-2 was used as a target cell type to test the transforming ability of a human papillomavirus (hpv-1a). to this end, molecularly cloned hpv-1a genomes were introduced into cultured rat-2 cells in cotransfection experiments using a cloned herpes simplex virus thymidine kinase gene as a selectable phenotypic maker. in each of 13 hpv-1a-positive cell clones examined the papillomavirus dna sequences were associated with the high molecular weight ...	1985	2987396
[detection of various papilloma virus types in cytologic smears of precancerous conditions and cancers of the uterine cervix].	dna-in situ hybridisation on epithelial cells taken from cervical swabs of 101 different patients cytologically diagnosed as iii d to v (munich classification 1975) were performed using 32p-labelled dna of the human papilloma virus (hpv) types 6, 11, 16 and 18 as smears. the following correlations between the cytological classification and dna hybridisation were obtained: 55 out of 58 women (94.8%) having a normal pap smear (pap ii) were negative by hybridisation as well, three cases contained h ...	1985	2989076
bowenoid papulosis. presence of human papillomavirus (hpv) structural antigens and of hpv 16-related dna sequences.	this study reviews 39 cases of anogenital bowenoid papulosis lesions in 22 individuals of both sexes that were analyzed clinically, histologically, immunocytochemically, and virologically. macroscopically, three different types of lesions were demonstrated: erythematous macules; papules (lichenoid and/or pigmented papules); and leukoplakialike lesions. microscopically, bowenoid papulosis fulfills the criteria of a squamous cell carcinoma in situ. much like oral precancers, three distinct growth ...	1985	2990353
papillomavirus genomes in human cervical tumors: analysis of their transcriptional activity.	four of six human cervical carcinoma biopsies were shown to contain the dna of the human papillomavirus type 16 (hpv-16) covalently linked with the tumor cell dna. hpv-16-specific mrna species were observed in only one of the four tumors. no such sequences were found in the three other specimens in conditions that permitted the detection of less than one mrna molecule per cell. it is concluded that maintenance of the malignant nature of these cervical tumors does not depend on the continuous tra ...	1985	2991933
comparison of prevalence of human papillomavirus antigen in biopsies from women with cervical intraepithelial neoplasia.	human papillomavirus antigen was found in 39 (16%) of 253 colposcopic biopsies from a group of women at high risk for cervical cancer who had been examined in the early 1970s. immunohistochemical evidence of papillomavirus infection was found in 20 (30%) of 67 cervical intraepithelial neoplasia lesions infected with wart virus from these patients. when these results were compared with results of a similar study carried out in the early 1980s there was no significant difference in the prevalence ...	1985	2993370
human papillomavirus dna in female condylomata.	we have studied the presence and state of human papillomavirus (hpv) genomes in condylomata acuminata of the vulva, vagina, and cervix and in flat condylomas of the cervix from japanese females by dna hybridization with hpv 6 and 11 dnas. unintegrated hpv 6-related dna (hpv 6 or 11 dna) was detected in 55 out of 60 condylomata acuminata and in 1 out of 4 flat condylomas under stringent hybridization conditions. the hpv dnas were identified as hpv 6 dna in 29 condylomata acuminata (20 vulvar, 3 v ...	1985	2993453
detection and localization of human papillomavirus dna in human genital condylomas by in situ hybridization with biotinylated probes.	we have examined the distribution of human papillomavirus (hpv) dna in paraffin sections of humans warts by in situ hybridization with biotin-labeled dna probes. recombinant plasmid dnas (hpv-1, -6, -11, -16) were labeled by nick translation with biotinylated deoxyuridine triphosphate. paraffin sections were hybridized with the probes for 18 h in stringent or non-stringent conditions, and dna-dna hybrids were detected by immunocytochemistry. paraffin sections of warts were also examined for the ...	1985	2993496
occurrence of human papillomavirus types 16 and 18 dna in cervical carcinomas from japan: age of patients and histological type of carcinomas.	we have detected by dna hybridization human papillomavirus (hpv) types 16 and 18 dna sequences in 19 and 3, respectively, out of 56 cervical carcinomas from japan. eighteen out of 19 hpv 16-positive specimens were from squamous cell carcinomas, whereas the three hpv 18-positive specimens were from a squamous cell carcinoma (1/50), an adenosquamous carcinoma (1/3), and an adenocarcinoma (1/3). the occurrence of hpv 16 dna decreased in patients over 60 years old (less than or equal to 60 years, 15 ...	1985	2995290
reproducibility of the cytologic diagnosis of human papillomavirus infection.	as part of a larger epidemiologic investigation of the association between human papillomavirus (hpv) infection and cervical intraepithelial neoplasia, the reliability of the cytologic diagnosis of hpv infection was examined. a random sample of cervicovaginal specimens with cytologic changes characteristic of hpv infection were matched with a second set of slides, with regard to the date and severity of the smear and the age of the woman from whom the smear was obtained. the kappa statistic for ...	1985	2996269