Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| bordetella pertussis filamentous hemagglutinin itself does not trigger anti-inflammatory interleukin-10 production by human dendritic cells. | filamentous hemagglutinin (fha) is an important adhesin of the whooping cough agent bordetella pertussis and is contained in most acellular pertussis vaccines. recently, fha was proposed to exert an immunomodulatory activity through induction of tolerogenic il-10 secretion from dendritic cells. we have re-evaluated the cytokine-inducing activity of fha, placing specific emphasis on the role of the residual endotoxin contamination of fha preparations. we show that endotoxin depletion did not affe ... | 2016 | 26699834 |
| the relationship between bordetella pertussis genotype and clinical severity in australian children with pertussis. | changes in circulating bordetella pertussis genotypes, including a novel pertussis toxin promoter ptxp3 allele and absence of pertactin (prn) antigen, have been reported from several countries but limited data on relative severity are available. we compared markers of disease severity in children with b. pertussis infection due to strains of differing genotype. | 2016 | 26675318 |
| adenylate cyclase toxin-mediated delivery of the s1 subunit of pertussis toxin into mammalian cells. | the adenylate cyclase toxin (act) of bordetella pertussis internalizes its catalytic domain into target cells. act can function as a tool for delivering foreign protein antigen moieties into immune effector cells to induce a cytotoxic t lymphocyte response. in this study, we replaced the catalytic domain of act with an enzymatically active protein moiety, the s1 (adp-ribosyltransferase) subunit of pertussis toxin (pt). the s1 moiety was successfully internalized independent of endocytosis into s ... | 2016 | 26607401 |
| emerging bordetella pertussis strains induce enhanced signaling of human pattern recognition receptors tlr2, nod2 and secretion of il-10 by dendritic cells. | vaccines against pertussis have been available for more than 60 years. nonetheless, this highly contagious disease is reemerging even in countries with high vaccination coverage. genetic changes of bordetella pertussis over time have been suggested to contribute to the resurgence of pertussis, as these changes may favor escape from vaccine-induced immunity. nonetheless, studies on the effects of these bacterial changes on the immune response are limited. here, we characterize innate immune recog ... | 2017 | 28076445 |
| predicting the presence of bacterial pathogens in the airways of primary care patients with acute cough. | bacterial testing of all patients who present with acute cough is not feasible in primary care. furthermore, the extent to which easily obtainable clinical information predicts bacterial infection is unknown. we evaluated the diagnostic value of clinical examination and testing for c-reactive protein and procalcitonin for bacterial lower respiratory tract infection. | 2017 | 27777252 |
| [multiple-locus variable number tandem repeat analysis of bordetella pertussis strains collected in the czech republic in 1967-2015: spread of a variant adapted to the population with a high vaccination coverage]. | to perform multiple-locus variable number tandem repeat analysis (mlva) of b. pertussis strains from the collection of the national reference laboratory for diphtheria and pertussis (nrl/dipe), national institute of public health (niph), prague. the study strains were isolated from clinical specimens collected mostly in the czech republic over a nearly 50-year period from 1967 to 2015 (june). the isolates from three periods characterized by different vaccination strategies and trends in pertussi ... | 2017 | 27467327 |
| [novel approaches to control the rise in pertussis cases]. | pertussis is a respiratory disease caused by the gram-negative encapsulated bacterium bordetella pertussis. despite the high vaccination coverage rate and addition of new booster doses to the immunisation scheme (in response to the epidemiological situation), pertussis is on the rise not only in the czech republic but also in many other countries. the age groups at highest risk are infants and, to a lower extent, newborns who can get infected before receiving the first dose of vaccine and develo ... | 2017 | 27467322 |
| clinical manifestations and molecular characterization of pertactin-deficient and pertactin-producing bordetella pertussis in children, philadelphia 2007-2014. | bordetella pertussis strains lacking expression of pertactin, a bacterial adhesin and vaccine target, are emerging. there are limited data on disease manifestations of mutant strains in children. we sought to compare clinical manifestations of pertactin-deficient and pertactin-producing b. pertussis infection in infants and describe corresponding molecular characteristics. | 2017 | 27624959 |
| prior exposure to bordetella species as an exclusion criterion in the baboon model of pertussis. | the baboon model of bordetella pertussis infection is the newest and most clinically accurate model of the human disease to date. however, among the 15 experimentally infected baboons in this study, a subset of baboons did not exhibit the expected high bacterial colonization levels or increase in white blood cell count. moreover, cultures of nasopharyngeal wash samples from several baboons suggested b. bronchiseptica coinfection. analysis of serum antibodies recognizing filamentous hemagglutinin ... | 2017 | 27666464 |
| th1 versus th2 t cell polarization by whole-cell and acellular childhood pertussis vaccines persists upon re-immunization in adolescence and adulthood. | the recent increase in cases of whooping cough among teenagers in the us suggests that the acellular bordetella pertussis vaccine (ap) that became standard in the mid 1990s might be relatively less effective than the whole-bacteria formulation (wp) previously used since the 1950s. to understand this effect, we compared antibody and t cell responses to a booster immunization in subjects who received either the wp or ap vaccine as their initial priming dose in childhood. antibody responses in wp- ... | 2017 | 27212461 |
| the pertussis hypothesis: bordetella pertussis colonization in the pathogenesis of alzheimer's disease. | while a number of endogenous risk factors including age and genetics are established for alzheimer's disease (ad), identification of acquired, potentially preventable or treatable causes, remains limited. in this paper, we review three epidemiologic case studies and present extensive biologic, immunologic and anatomic evidence to support a novel hypothesis that bordetella pertussis (bp), the bacterium better known to cause whooping cough, is an important potential cause of ad. cross-cultural doc ... | 2017 | 27692981 |
| randomized clinical trial of the safety and immunogenicity of the tdap vaccine in pregnant mexican women. | immunization with the tetanus, diphtheria, and pertussis (tdap) vaccine raises controversies on immunogenicity and possible antibody interference. we performed an experimental, double-blind, parallel group controlled clinical trial to evaluate the safety and immunogenicity of the tdap vaccine in 204 pregnant women and their children and to determine its interference in antibody production. pregnant women 18 to 38 y of age with 12 to 24 weeks gestation, a low obstetric risk, and without serious d ... | 2017 | 27686182 |
| use of a toxin neutralization assay to characterize the serologic response to adenylate cyclase toxin after infection with bordetella pertussis. | adenylate cyclase toxin (act) is an essential virulence factor of bordetella pertussis, and antibodies to act protect against b. pertussis infection in mice. the toxin is therefore a strong candidate antigen for addition to future acellular pertussis vaccines. in order to characterize the functionality of the immunologic response to act after infection, we developed an assay for testing the ability of serum samples from subjects infected with b. pertussis to neutralize act-induced cytotoxicity i ... | 2017 | 27760780 |
| identification of active constraints in dynamic flux balance analysis. | this study deals with the calibration of dynamic metabolic flux models that are formulated as the maximization of an objective subject to constraints. two approaches were applied for identifying the constraints from data. in the first approach a minimal active number of limiting constraints is found based on data that are assumed to be bounded within sets whereas, in the second approach, the limiting constraints are found based on parametric sensitivity analysis. the ability of these approaches ... | 2017 | 27790866 |
| modulation of pertussis and adenylate cyclase toxins by sigma factor rpoe in bordetella pertussis. | bordetella pertussis is a human pathogen that can infect the respiratory tract and cause the disease known as whooping cough. b. pertussis uses pertussis toxin (pt) and adenylate cyclase toxin (act) to kill and modulate host cells to allow the pathogen to survive and persist. b. pertussis encodes many uncharacterized transcription factors, and very little is known about their functions. rpoe is a sigma factor which, in other bacteria, responds to oxidative, heat, and other environmental stresses ... | 2017 | 27849178 |
| reduction of pertussis inflammatory pathology by therapeutic treatment with sphingosine-1-phosphate receptor ligands by a pertussis toxin-insensitive mechanism. | recent data have demonstrated the potential of sphingosine 1-phosphate (s1p) receptor (s1pr) agonism in the treatment of infectious diseases. a previous study used a murine model of bordetella pertussis infection to demonstrate that treatment with the s1pr agonist aal-r reduces pulmonary inflammation during infection. in the current study, we showed that this effect is mediated via the s1pr1 on lysm+ (myeloid) cells. signaling via this receptor results in reduced lung inflammation and cellular r ... | 2017 | 27815382 |
| identifying children at risk of malignant bordetella pertussis infection. | to identify factors associated with malignant pertussis. | 2017 | 27811532 |
| bordetella adenylate cyclase toxin interacts with filamentous haemagglutinin to inhibit biofilm formation in vitro. | bordetella pertussis, the causative agent of whooping cough, secretes and releases adenylate cyclase toxin (act), which is a protein bacterial toxin that targets host cells and disarms immune defenses. act binds filamentous haemagglutinin (fha), a surface-displayed adhesin, and until now, the consequences of this interaction were unknown. a b. bronchiseptica mutant lacking act produced more biofilm than the parental strain; leading irie et al. to propose the act-fha interaction could be responsi ... | 2017 | 27731909 |
| il-17-producing innate and pathogen-specific tissue resident memory γδ t cells expand in the lungs of bordetella pertussis-infected mice. | γδ t cells play a role in protective immunity to infection at mucosal surface, but also mediate pathology in certain autoimmune diseases through innate il-17 production. recent reports have suggested that γδ t cells can have memory analogous to conventional αβ t cells. in this study we have examined the role of γδ t cells in immunity to the respiratory pathogen bordetella pertussis γδ t cells, predominantly vγ4(-)γ1(-) cells, produced il-17 in the lungs as early as 2 h after infection. the bacte ... | 2017 | 27864475 |
| does breastfeeding protect young infants from pertussis? case-control study and immunologic evaluation. | pertussis infection can be severe in unvaccinated infants. a case-control study was conducted to investigate the potential role of breastfeeding in protecting young, unvaccinated infants from pertussis. | 2017 | 27870812 |
| adverse events following primary and secondary immunisation with whole-cell pertussis: a systematic review protocol. | pertussis is a contagious respiratory illness caused by the bacterium bordetella pertussis. two types of vaccines are currently available against the disease: whole-cell pertussis (wp) and acellular pertussis (ap). with the shift of high-income countries from wp to ap as a result of adverse events following immunisation (aefi), an upsurge in reported cases of pertussis has been noticed. owing to this, it is proposed to use wp as a prime and ap for boost vaccination strategy. however, a compariso ... | 2017 | 28122832 |
| bordetella pertussis infection in children with severe pneumonia, philippines, 2012-2015. | a case-comparison study was conducted based on an observational study of severe pneumonia among hospitalized children in the philippines. the children, from 8days to 13years old and hospitalized with clinical diagnosis of severe or very severe pneumonia from august 2012 to february 2015, were recruited. nasopharyngeal swabs were collected from 1152 cases and b. pertussis were detected from 34 cases by pcr. pertussis-positive cases were more likely to have no fever, more than one week of coughing ... | 2017 | 28109708 |
| a new vaccine delivery vehicle and adjuvant candidate: bordetella pertussis inactivated whole cells entrapped in alginate microspheres. | there is no doubt about whole cell pertussis vaccine efficacy, but it is necessary to improve the vaccine quality specially to decreasing its toxicity by obtaining good immunogenicity with low bacteria number content. in this work, under optimum condition inactivated b. pertussis bacteria cells entrapped alginate microparticles were fabricated and in vivo immunogenicity and potency of new microparticle based vaccine evaluated in mice. microspheres loaded inactive b. pertussis bacterium cells wer ... | 2017 | 28079006 |
| population-based pertussis incidence and risk factors in infants less than 6 months in nepal. | pertussis is estimated to cause 2 percent of childhood deaths globally and is a growing public health problem in developed countries despite high vaccination coverage. infants are at greatest risk of morbidity and mortality. maternal vaccination during pregnancy may be effective to prevent pertussis in young infants, but population-based estimates of disease burden in infants are lacking, particularly in low-income countries. the objective of this study was to estimate the incidence of pertussis ... | 2017 | 28073985 |
| risk factors for pertussis in adults and teenagers in england. | pertussis is a vaccine-preventable respiratory infection caused by bordetella pertussis which can be fatal in infants. although high vaccine coverage led to prolonged disease control in england, a national outbreak of pertussis in 2011 led to the largest increase in over two decades, including a marked increase in cases aged ⩾15 years. a case-control study in four regions of england was undertaken to investigate risk factors for pertussis in adolescents and adults, specifically employment type a ... | 2017 | 28065204 |
| characteristics of bordetella pertussis infection among infantsand children admitted to paediatric intensive care units in greece: a multicentre, 11-year study. | to describe children with pertussis who required intensive care. | 2017 | 28058755 |
| whooping cough in adults: a series of severe cases. | bordetella pertussis is a gram-negative aerobic coccobacillus causing contagious respiratory tract disease called whooping cough. the virulence factors consist of pertussis toxin, filamentous hemagglutinin, fimbriae, lipooligosaccharide, and adenylate cyclase toxin. the disease causes a worldwide threat to public health despite a high vaccination coverage. the course of whooping cough in adults is frequently atypical, causing difficulty in diagnosis. in this report we present five patients hospi ... | 2017 | 28039663 |
| camp signaling of adenylate cyclase toxin blocks the oxidative burst of neutrophils through epac-mediated inhibition of phospholipase c activity. | the adenylate cyclase toxin-hemolysin (cyaa) plays a key role in immune evasion and virulence of the whooping cough agent bordetella pertussis. cyaa penetrates the complement receptor 3-expressing phagocytes and ablates their bactericidal capacities by catalyzing unregulated conversion of cytosolic atp to the key second messenger molecule camp. we show that signaling of cyaa-generated camp blocks the oxidative burst capacity of neutrophils by two converging mechanisms. one involves camp/protein ... | 2017 | 28039302 |
| acylation of the bordetella pertussis cyaa-hemolysin: functional implications for efficient membrane insertion and pore formation. | previously, the ~130-kda cyaa-hemolysin domain (cyaa-hly) from bordetella pertussis co-expressed with cyac-acyltransferase in escherichia coli was demonstrated to be palmitoylated at lys(983) and thus activated its hemolytic activity against target erythrocytes. here, we report the functional importance of lys(983)-palmitoylation for membrane insertion and pore formation of cyaa-hly. intrinsic fluorescence emissions of both non-acylated cyaa-hly (na/cyaa-hly) and cyaa-hly were indistinguishable, ... | 2017 | 27993565 |
| proteome analysis is a valuable tool to monitor antigen expression during upstream processing of whole-cell pertussis vaccines. | physicochemical and immunochemical assays were applied to substantiate the relation between upstream processing and the quality of whole-cell pertussis vaccines. bordetella pertussis bacteria were cultured on a chemically defined medium using a continuous cultivation process in stirred tank reactors to obtain uniform protein expression. continuous culture favors the consistent production of proteins known as virulence factors. magnesium sulfate was added during the steady state of the culture in ... | 2017 | 27977922 |
| evaluation of a commercial loop-mediated isothermal amplification assay for diagnosis of bordetella pertussis infection. | we evaluated a commercial loop-mediated isothermal amplification (lamp) assay kit for bordetella pertussis detection. the lamp primers were designed to target the ptxp1 allele of the pertussis toxin promoter, but the assay could detect b. pertussis ptxp3 and ptxp8 strains in addition to ptxp1 strains, with high analytical sensitivity. | 2017 | 27974227 |
| antimicrobial susceptibility and molecular detection of pertactin-producing and pertactin-deficient bordetella pertussis. | resurgence of bordetella pertussis in recent years in the united states has coincided with a dramatic rise in pertactin-deficient strains. limited data exist on detectability by nucleic acid amplification testing and antimicrobial susceptibility of pertactin-deficient b. pertussis. this study compares 15 pertactin-producing and 15 pertactin-deficient b. pertussis isolates. pertactin-producing and pertactin-deficient strains were equally detected by nucleic acid amplification testing and were sus ... | 2017 | 27956730 |
| drivers of airborne human-to-human pathogen transmission. | airborne pathogens - either transmitted via aerosol or droplets - include a wide variety of highly infectious and dangerous microbes such as variola virus, measles virus, influenza a viruses, mycobacterium tuberculosis, streptococcus pneumoniae, and bordetella pertussis. emerging zoonotic pathogens, for example, mers coronavirus, avian influenza viruses, coxiella, and francisella, would have pandemic potential were they to acquire efficient human-to-human transmissibility. here, we synthesize in ... | 2017 | 27918958 |
| whole-genome sequencing and comparative genomic analysis of bordetella pertussis isolates from the 2007-2008 epidemic in israel. | 2017 | 27914992 | |
| significant decrease in pertactin-deficient bordetella pertussis isolates, japan. | prevalence of pertactin-lacking bordetella pertussis isolates has been observed worldwide. in japan, however, we found that the frequency of pertactin-deficient isolates in 2014-2016 (8%) was significantly lower than the frequency in 2005-2007 (41%), 2008-2010 (35%), and 2011-2013 (25%). this reduction was closely associated with changes in genotypes. | 2017 | 28322702 |
| gtl001, a bivalent therapeutic vaccine against human papillomavirus 16 and 18, induces antigen-specific cd8+ t cell responses leading to tumor regression. | prophylactic vaccines are available for women and girls not yet infected with hpv, but women already infected with hpv need a treatment to prevent progression to high-grade cervical lesions and cancer. gtl001 is a bivalent therapeutic vaccine for eradicating hpv-infected cells that contains hpv16 e7 and hpv18 e7 both fused to detoxified adenylate cyclase from bordetella pertussis, which binds specifically to cd11b+ antigen-presenting cells. this study examined the ability of therapeutic vaccinat ... | 2017 | 28301611 |
| what is wrong with pertussis vaccine immunity? the problem of waning effectiveness of pertussis vaccines. | pertussis is resurgent in some countries, particularly those in which children receive acellular pertussis (ap) vaccines in early infancy and boosters later in life. immunologic studies show that, whereas whole-cell pertussis (wp) vaccines orient the immune system toward th1/th17 responses, acellular pertussis vaccines orient toward th1/th2 responses. although ap vaccines do provide protection during the first years of life, the change in t-cell priming results in waning effectiveness of ap as e ... | 2017 | 28289064 |
| what is wrong with pertussis vaccine immunity? inducing and recalling vaccine-specific immunity. | the high incidence of pertussis in vaccinated adolescents suggests the failing of immune memory. we argue that acellular pertussis vaccines generate memory cells that are effectively reactivated by boosters better than by bordetella pertussis exposure. we propose that there are two main causes. one is the induction of vaccine-specific immunity rather than pathogen-specific immunity. the second is that strictly mucosal infections such as b. pertussis poorly reactivate memory b and t cells residin ... | 2017 | 28289058 |
| [investigation of seropositivity of bordetella pertussis in adults in a university hospital]. | whooping cough is a vaccine-preventable infectious diseases caused by bordetella pertussis/parapertussis. despite of routine immunization programs in the world, pertussis still remains endemic. recently unvaccinated or partially immunized infants have infected with this pathogen and also increase of incidence was observed in adolescents and adults. the source of pertussis in newborns are attributed to household, especially due to the family members. theaim of this study was to determine b.pertus ... | 2017 | 28283011 |
| recurrent apnea in an infant with pertussis due to household transmission. | bordetella pertussis causes life-threatening apnea in infants. lymphocytosis is an important clue for diagnosis and for determining the severity of pertussis. antibiotics do not shorten or ameliorate the disease and only decrease the risk of transmission. antepartum maternal immunization is important for preventing pertussis in infants. | 2017 | 28265381 |
| letter to the editor: verification of the rida(®)gene bordetella real-time pcr kit for the detection of bordetella pertussis and other bordetella species from nasopharyngeal swabs collected in regan-lowe transport medium. | 2017 | 28249928 | |
| safety evaluation of a vaccine: effect in maternal reproductive outcome and fetal anomaly frequency in rats using a leishmanial vaccine as a model. | while the immunogenic potential of the vaccination against infectious diseases was extensively shown, data on the safety assessment of recombinant proteins in vaccine formulations administered during pregnancy are still scarce. in the current study, the antigenicity of a vaccine against leishmaniasis (based on leishmania braziliensis recombinant protein peroxidoxin) during pregnancy and possible maternal reproductive outcomes and fetal anomalies after immunization with a leishmanial vaccine or a ... | 2017 | 28249007 |
| meta-analysis of pulmonary transcriptomes from differently primed mice identifies molecular signatures to differentiate immune responses following bordetella pertussis challenge. | respiratory infection with bordetella pertussis leads to severe effects in the lungs. the resulting immunity and also immunization with pertussis vaccines protect against disease, but the induced type of immunity and longevity of the response are distinct. in this study the effects of priming, by either vaccination or infection, on a subsequent pathogen encounter were studied. to that end, three postchallenge transcriptome datasets of previously primed mice were combined and compared to the resp ... | 2017 | 28243609 |
| characterisation of the bordetella pertussis secretome under different media. | our understanding of the bordetella pertussis secretome remains limited including the role of different growth conditions in the secretome. in this study the secretome of l1423, a clinical isolate from the 2008-2012 australian epidemic, cultured on stainer-scholte (ss) and thalen-ijssel (thijs) media for 12h was characterised using liquid chromatography-mass spectrometry (lc-ms/ms). in the supernatant, lc-ms/ms identified 260 proteins with 143 bioinformatically predicted to be secreted. eighty p ... | 2017 | 28242451 |
| zn(2+)-dependent autocatalytic activity of the bordetella pertussis cyaa-hemolysin. | proteolytic degradation of the ∼100-kda isolated rtx (repeat-in-toxin) subdomain (cyaa-rtx) of the bordetella pertussis cyaa-hemolysin (cyaa-hly) was evidently detected upon solely-prolonged incubation. here, a truncated cyaa-hly fragment (cyaa-hp/bi) containing hydrophobic and acylation regions connected with the first rtx block (bi1015-1088) was constructed as a putative precursor for investigating its potential autocatalysis. the 70-kda his-tagged cyaa-hp/bi fragment which was over-expressed ... | 2017 | 28238785 |
| short-read whole genome sequencing for laboratory-based surveillance of bordetella pertussis. | bordetella pertussis (bp) is a gram-negative bacterium that causes respiratory infections in humans. ongoing molecular surveillance of bp acellular vaccine (ap) antigens is critical for understanding the interaction between evolutionary pressures, disease pathogenesis, and vaccine effectiveness. methods currently used to characterize ap components are relatively labour intensive and low throughput. to address this challenge, we sought to derive ap antigen genotypes from minimally processed short ... | 2017 | 28228490 |
| the characterization of bordetella pertussis strains isolated in the central-western region of brazil suggests the selection of a specific genetic profile during 2012-2014 outbreaks. | pertussis is a worldwide acute respiratory disease caused by the bacterium bordetella pertussis. despite high vaccine coverage, the bacterium continues to circulate in populations and is still one of the most common vaccine-preventable diseases. in brazil, pertussis incidence has presented a significant decrease since 1990 but since 2011 a sudden increase in incidence has been observed. thus, the aim of this study was to perform a molecular epidemiological characterization of b. pertussis strain ... | 2017 | 28219454 |
| gtl001 and bivalent cyaa-based therapeutic vaccine strategies against human papillomavirus and other tumor-associated antigens induce effector and memory t-cell responses that inhibit tumor growth. | gtl001 is a bivalent therapeutic vaccine containing human papillomavirus (hpv) 16 and hpv18 e7 proteins inserted in the bordetella pertussis adenylate cyclase (cyaa) vector intended to prevent cervical cancer in hpv-infected women with normal cervical cytology or mild abnormalities. to be effective, therapeutic cervical cancer vaccines should induce both a t cell-mediated effector response against hpv-infected cells and a robust cd8(+) t-cell memory response to prevent potential later infection. ... | 2017 | 28196735 |
| stability, structural and functional properties of a monomeric, calcium-loaded adenylate cyclase toxin, cyaa, from bordetella pertussis. | bordetella pertussis, the causative agent of whooping cough, secretes an adenylate cyclase toxin, cyaa, which invades eukaryotic cells and alters their physiology by camp overproduction. calcium is an essential cofactor of cyaa, as it is the case for most members of the repeat-in-toxins (rtx) family. we show that the calcium-bound, monomeric form of cyaa, hcyaam, conserves its permeabilization and haemolytic activities, even in a fully calcium-free environment. in contrast, hcyaam requires sub-m ... | 2017 | 28186111 |
| fine epitope mapping of two antibodies neutralizing the bordetella adenylate cyclase toxin. | adenylate cyclase toxin (act) is an important bordetella pertussis virulence factor that is not included in current acellular pertussis vaccines. we previously demonstrated that immunization with the repeat-in-toxin (rtx) domain of act elicits neutralizing antibodies in mice and discovered the first two antibodies to neutralize act activities by occluding the receptor-binding site. here, we fully characterize these antibodies and their epitopes. both antibodies bind act with low nanomolar affini ... | 2017 | 28177609 |
| protective role of passively transferred maternal cytokines against bordetella pertussis infection in newborn piglets. | maternal vaccination represents a potential strategy to protect both the mother and the offspring against life-threatening infections. this protective role has mainly been associated with antibodies, but the role of cell-mediated immunity, in particular passively transferred cytokines, is not well understood. here, using a pertussis model, we have demonstrated that immunization of pregnant sows with heat-inactivated bacteria leads to induction of a wide range of cytokines (e.g., tumor necrosis f ... | 2017 | 28167667 |
| the history of bordetella pertussis genome evolution includes structural rearrangement. | despite high pertussis vaccine coverage, reported cases of whooping cough (pertussis) have increased over the last decade in the united states and other developed countries. although bordetella pertussis is well known for its limited gene sequence variation, recent advances in long-read sequencing technology have begun to reveal genomic structural heterogeneity among otherwise indistinguishable isolates, even within geographically or temporally defined epidemics. we have compared rearrangements ... | 2017 | 28167525 |
| immune response and protective profile elicited by a multi-epitope chimeric protein derived from leptospira interrogans. | pathogenic leptospira is the causative agent of leptospirosis, a widely disseminated disease of human and veterinary concern. the development of vaccines that elicit cross-protective immunity through multiple leptospiral serovars has long been pursued. the aim of this study was to develop a novel chimeric multi-epitope fusion antigen, containing sequences of previously studied outer membrane proteins (omps) of leptospira. | 2017 | 28161462 |
| the proline residue at position 319 of bvgs is essential for bvgas activation in bordetella pertussis. | 2017 | 28158456 | |
| dot immunoassay for the simultaneous determination of postvaccination immunity against pertussis, diphtheria, and tetanus. | a dot immunoassay for simultaneous semiquantitative detection of igg against tetanus toxoid (ttx) and diphtheria toxoid (dtx) and qualitative detection of anti-bordetella pertussis iggs in human blood serum using carbon nanoparticles functionalized with streptococcal protein g was developed. inactivated b. pertussis cells in suspension form were used as an antigen in the immunoassay. pertussis, tetanus, and diphtheria antigens were separately spotted onto nitrocellulose strips, and then the immu ... | 2017 | 28374129 |
| analysis of membrane protein interactions with a bacterial adenylate cyclase-based two-hybrid (bacth) technique. | the bacterial two-hybrid (bacth, for "bacterial adenylate cyclase-based two-hybrid") technique is a simple and fast genetic approach to analyze protein-protein interactions in vivo. in this system, the proteins of interest are genetically fused to two complementary fragments from the catalytic domain of bordetella pertussis adenylate cyclase and co-expressed in strains of escherichia coli deficient in adenylate cyclase. association of the hybrid proteins restores synthesis of cyclic amp (camp), ... | 2017 | 28369675 |
| bordetella pertussis infections in travelers: data from the geosentinel global network. | pertussis is a highly contagious, vaccine-preventable respiratory infection that is endemic worldwide. there are limited data regarding the occurrence of pertussis in travelers. the objective of this study is to identify travel-related pertussis cases reported to the geosentinel surveillance network. | 2017 | 28355615 |
| assessment of safety and efficacy against bordetella pertussis of a new tetanus-reduced dose diphtheria-acellular pertussis vaccine in a murine model. | tetanus-reduced dose diphtheria-acellular pertussis (tdap) vaccination during adolescence was introduced in response to the resurgence of pertussis in various countries. a new tdap vaccine was manufactured in korea as a countermeasure against a predicted tdap vaccine shortage. this study was performed to evaluate the immunogenicity, safety, and protection efficacy against bordetella pertussis of the new tdap vaccine in a murine model. | 2017 | 28376777 |
| seroprevalence of bordetella pertussis antibodies in adults in hungary: results of an epidemiological cross-sectional study. | pertussis (whooping cough) is well known to be underreported, particularly among adults, who can act as an infectious reservoir, potentially putting susceptible newborns at risk of serious illness. the purpose of this study was to estimate the seroprevalence of pertussis in adults in hungary. | 2017 | 28376739 |
| the extent of the temperature-induced membrane remodeling in two closely related bordetella species reflects their adaptation to diverse environmental niches. | changes in environmental temperature represent one of the major stresses faced by microorganisms as it affects the function of the cytoplasmic membrane. in this study we have analyzed the thermal adaptation in two closely related respiratory pathogens bordetella pertussis and bordetella bronchiseptica. while b. pertussis represents a pathogen strictly adapted to the human body temperature, b. bronchiseptica causes infection in a broad range of animals and survives also outside of the host. we ap ... | 2017 | 28348085 |
| bordetella plrsr regulatory system controls bvgas activity and virulence in the lower respiratory tract. | bacterial pathogens coordinate virulence using two-component regulatory systems (tcs). the bordetella virulence gene (bvgas) phosphorelay-type tcs controls expression of all known protein virulence factor-encoding genes and is considered the "master virulence regulator" in bordetella pertussis, the causal agent of pertussis, and related organisms, including the broad host range pathogen bordetella bronchiseptica we recently discovered an additional sensor kinase, plrs [for persistence in the low ... | 2017 | 28167784 |
| essential role of bordetella nadc in a quinolinate salvage pathway for nad biosynthesis. | nicotinamide adenine dinucleotide (nad) is produced via de novo biosynthesis pathways and by salvage or recycling routes. the classical bordetella bacterial species are known to be auxotrophic for nicotinamide or nicotinic acid. this study confirmed that bordetella bronchiseptica, bordetella pertussis and bordetella parapertussis have the recycling/salvage pathway genes pnca and pncb, for use of nicotinamide or nicotinic acid, respectively, for nad synthesis. although these bordetellae lack the ... | 2017 | 27783449 |
| interaction of bacteroides fragilis toxin with outer membrane vesicles reveals new mechanism of its secretion and delivery. | the only recognized virulence factor of enterotoxigenic bacteroides fragilis (etbf) that accompanies bloodstream infections is the zinc-dependent non-lethal metalloprotease b. fragilis toxin (bft). the isolated toxin stimulates intestinal secretion, resulting in epithelial damage and necrosis. numerous publications have focused on the interrelation of bft with intestinal inflammation and colorectal neoplasia, but nothing is known about the mechanism of its secretion and delivery to host cells. h ... | 2017 | 28144586 |
| a unilateral hyperlucent lung - swyer-james syndrome: a case report and literature review. | swyer-james-macleod syndrome (sjms) is a rare etiology of a unilateral hyperlucent hemithorax but an important one, which should be considered in any individual with such findings. presentation usually occurs in adulthood with an asymptomatic history in many cases or with a history of childhood infections. clinically, symptomatic patients may present with productive cough, dyspnea on exertion, hemoptysis, decreased exercise tolerance and recurrent pulmonary infections. many individuals are asymp ... | 2017 | 28138424 |
| pseudomonas aeruginosa pore-forming exolysin and type iv pili cooperate to induce host cell lysis. | clinical strains of pseudomonas aeruginosa lacking the type iii secretion system genes employ a toxin, exolysin (exla), for host cell membrane disruption. here, we demonstrated that exla export requires a predicted outer membrane protein, exlb, showing that exla and exlb define a new active two-partner secretion (tps) system of p. aeruginosa in addition to the tps signals, exla harbors several distinct domains, which include one hemagglutinin domain, five arginine-glycine-aspartic acid (rgd) mot ... | 2017 | 28119472 |
| impact of age and vaccination history on long-term serological responses after symptomatic b. pertussis infection, a high dimensional data analysis. | capturing the complexity and waning patterns of co-occurring immunoglobulin (ig) responses after clinical b. pertussis infection may help understand how the human host gradually loses protection against whooping cough. we applied bi-exponential modelling to characterise and compare b. pertussis specific serological dynamics in a comprehensive database of igg, igg subclass and iga responses to ptx, fha, prn, fim2/3 and omv antigens of (ex-) symptomatic pertussis cases across all age groups. the d ... | 2017 | 28091579 |
| single- and multiple viral respiratory infections in children: disease and management cannot be related to a specific pathogen. | the number of viral pathogens associated with pediatric acute respiratory tract infection (ari) has grown since the introduction of reverse transcription real-time polymerase chain reaction (rt-pcr) assays. multiple viruses are detected during a single ari episode in approximately a quarter of all cases. the clinical relevance of these multiple detections is unclear, as is the role of the individual virus. we therefore investigated the correlation between clinical data and rt-pcr results in chil ... | 2017 | 28077074 |
| broad-range detection of microorganisms directly from bronchoalveolar lavage specimens by pcr/electrospray ionization-mass spectrometry. | the clinical demand on rapid microbiological diagnostic is constantly increasing. pcr coupled to electrospray ionization-mass spectrometry, pcr/esi-ms, offers detection and identification of over 750 bacteria and candida species directly from clinical specimens within 6 hours. in this study, we investigated the clinical performance of the iridica bac lrt assay for detection of bacterial pathogens in 121 bronchoalveolar lavage (bal) samples that were received consecutively at our bacterial labora ... | 2017 | 28085931 |
| modeling diversity in structures of bacterial outer membrane lipids. | lipopolysaccharides (lpss) are vital components of the outer membrane of gram-negative bacteria, and they act as extremely strong stimulators of innate immunity in diverse eukaryotic species. the primary immunostimulatory center of the lps molecule is lipid a, a disaccharide-bound lipophilic domain. considering the broad diversity in bacterial species, there are variations in the lipid a structures and their immunogenic potencies. in this work, we model the lipid a structures of eight commensal ... | 2017 | 28080049 |
| evasion of neutrophil extracellular traps by respiratory pathogens. | the release of neutrophil extracellular traps (nets) is a major immune mechanism intended to capture pathogens. these histone- and protease-coated dna structures are released by neutrophils in response to a variety of stimuli, including respiratory pathogens, and have been identified in the airways of patients with respiratory infection, cystic fibrosis, acute lung injury, primary graft dysfunction, and chronic obstructive pulmonary disease. net production has been demonstrated in the lungs of m ... | 2017 | 27854516 |
| functional contributions of positive charges in the pore-lining helix 3 of the bordetella pertussis cyaa-hemolysin to hemolytic activity and ion-channel opening. | the bordetella pertussis cyaa-hemolysin (cyaa-hly) domain was previously demonstrated to be an important determinant for hemolysis against target erythrocytes and ion-channel formation in planar lipid bilayers (plbs). here, net-charge variations in the pore-lining helix of thirteen related rtx cytolysins including cyaa-hly were revealed by amino acid sequence alignments, reflecting their different degrees of hemolytic activity. to analyze possible functional effects of net-charge alterations on ... | 2017 | 28300777 |